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  • novel
  • The here presented generation of an analog-sensitive CDKA;1 version is functional and represent a novel tool to modulate kinase activity in vivo and identify kinase substrates. (biomedcentral.com)
  • yeast
  • Boulton TG, Yancopoulos GD, Gregory JS, Slaughter C. Moomaw C, Hsu J, Cobb MH (1990) An insulin-stimulated protein kinase similar to yeast kinases involved in cell cycle control. (springer.com)
  • Here we have applied a chemical genetic approach and generated an analog-sensitive version of CDKA;1, the central cell-cycle regulator in Arabidopsis and homolog of the yeast Cdc2/CDC28 kinases. (biomedcentral.com)
  • Substantial work in yeast and animal model systems has shown that high kinase activity levels are in particular required to promote the transition from a gap phase (G1) into S phase where the nuclear DNA becomes replicated and from a second gap phase (G2) into M phase (mitosis) during which the chromosomes are distributed to the newly forming daughter cells. (biomedcentral.com)
  • Furthermore, human CDC2 is capable of rescuing fission yeast carrying a cdc2 mutation. (wikipedia.org)
  • substrates
  • Thus, identification of kinase substrates is pivotal for the understanding of many - if not all - molecular biological processes. (biomedcentral.com)
  • A pilot set of five proteins involved in a range of different processes could be confirmed in independent kinase assays to be phosphorylated by CDKA;1 approving the applicability of the here-developed method to identify substrates. (biomedcentral.com)
  • Proteins
  • SR protein-specific kinase 1 (SRPK1) and SRPK2 are regulated by the cell cycle and are specific for SR proteins ( 3 ). (aacrjournals.org)
  • The SRPK family of kinases, containing bipartite kinase domains separated by a unique spacer, is mainly localized in the cytoplasm, which is critical for nuclear import of SR proteins in a phosphorylation-dependent manner. (aacrjournals.org)
  • The two signaling node proteins cyclin-dependent kinase 2 and the actin regulator neuronal Wiskott-Aldrich syndrome protein have qualitatively similar signaling properties. (nih.gov)
  • Protein and lipid kinases regulate many different cell processes by aHrffpg phosphate groups to targets such as proteins or lipids. (allindianpatents.com)
  • CDKs are relatively small proteins, with molecular weights ranging from 34 to 40 kDa, and contain little more than the kinase domain. (wikipedia.org)
  • Importantly, these C-CDK complexes act as a kinase, phosphorylating and inactivating the protein of Rb and p-Rb related "pocket proteins" p107 and p130. (wikipedia.org)
  • Regulation
  • Proline-rich (PxxP) motifs in HIV-1 Nef bind to SH3 domains of a subset of Src kinases and are required for the enhanced growth of Nef+ viruses but not for down-regulation. (naver.com)
  • inhibits
  • Lactoferrin inhibits G1 cyclin-dependent kinases during growth arrest of human breast carcinoma cells. (biomedsearch.com)
  • Overexpression of ErbB2 blocks Taxol-induced apoptosis by upregulation of p21Cip1, which inhibits p34Cdc2 kinase. (nih.gov)
  • activates
  • Taxol activates p34Cdc2 kinase in MDA-MB-435 breast cancer cells, leading to cell cycle arrest at the G2/M phase and, subsequently, apoptosis. (nih.gov)
  • cleft
  • The active site, or ATP-binding site, of all kinases is a cleft between a small amino-terminal lobe and a larger carboxy-terminal lobe. (wikipedia.org)
  • cellular
  • The MAP kinase signaling cascade Ras/Raf/MEK/ERK has been involved in a large variety of cellular and physiological processes that are crucial for life. (frontiersin.org)
  • This kinase has been shown to play a role in cellular proliferation. (wikipedia.org)
  • subfamily
  • Protein kinases, with at least 400 identified, constitute the largest subfamily of structurally related phosphoryi transferases and are responsible for the control of a wide variety of signal transduction processes within the cell. (allindianpatents.com)
  • mitosis
  • It is a kinase determining the timepoint of entry into mitosis, thus influencing the size of the daughter cells. (wikipedia.org)