Recombinant Cynomolgus NKG2A&CD94 Protein is expressed from HEK293 with His tag and Flag tag at the C-terminal.It contains Pro94-Leu233(NKG2A)&Lys32-Ile179(CD94). (watson-bio.cn)
Immobilized Cynomolgus NKG2A&CD94, His Tag at 1ug/ml (100ul/well) on the plate. (watson-bio.cn)
On healthy cells the non-classical HLA class Ib molecule HLA-E displays the cognate ligand for the NK cell receptor NKG2A/CD94 when bound to HLA class I signal peptide sequences. (mpg.de)
In the present study we have evaluated the molecular basis for the impaired NK cell inhibition by these peptides and determined the cell surface stability of individual p:HLA-E complexes and their binding efficiency to soluble NKG2A/CD94 or NKG2C/CD94 receptors. (mpg.de)
Receptor4
However, the contributions to immune evasion of HLA-E, a ligand for the inhibitory CD94/NKG2A receptor, when expressed on tumors, compared with adjacent tissue and peripheral blood mononuclear cells, remains unclear. (ox.ac.uk)
We also found that the presence of CD94/NKG2A on human tumor-specific T cells impairs IL2 receptor-dependent proliferation, which affects IFNγ-mediated responses and antitumor cytotoxicity. (ox.ac.uk)
Binding of the CD94/NKG2A receptor by its ligand (Qa-1(b)) did not significantly inhibit CD8(+) T cell effector functions. (duke.edu)
In a pathogenic situation when HLA class I is absent, HLA-E is bound to a diverse set of peptides and enables the stimulatory NKG2C/CD94 receptor to bind. (mpg.de)
Peptides2
Activated synovial NK cells produced interferon-gamma and tumour necrosis factor, and the production was further up-regulated by antibody masking of CD94/NKG2A, and down-regulated by target cells expressing human leucocyte antigen-E in complex with peptides known to engage CD94/NKG2A. (ox.ac.uk)
We show that non-canonical peptides provide stable cell surface expression of HLA-E, and these p:HLA-E complexes still bind to NKG2/CD94 receptors in a peptide-restricted fashion. (mpg.de)
Inhibitory2
Activating and inhibitory receptors on synovial fluid natural killer cells of arthritis patients: role of CD94/NKG2A in control of cytokine secretion. (ox.ac.uk)
Our results suggest that enriched HLA-E:CD94/NKG2A inhibitory interaction can impair survival of PD-1high TILs in the tumor microenvironment. (ox.ac.uk)
Cells3
We conclude that synovial NK cells have an activated phenotype and that CD94/NKG2A is a key regulator of synovial NK-cell cytokine synthesis. (ox.ac.uk)
The expression of CD94/NKG2A was rapidly initiated in naive CD8(+) T cells responding to peptide Ags in vitro and on many of the naive T cells that proliferate when transferred into lymphopenic (Rag-1(-/-)) hosts. (duke.edu)
These subtle effects suggest that CD94/NKG2A-mediated inhibition of T cells may be limited to particular circumstances or may synergize with other receptors that are similarly up-regulated. (duke.edu)
Cell2
Thus, CD94/NKG2A expression is a common consequence of CD8(+) T cell activation. (duke.edu)
However, expression of CD94 and NKG2A transgenes partially inhibited early events of T cell activation. (duke.edu)
Expression1
CD94/NKG2A is exclusively expressed on PD-1high TILs while lacking intratumoral CD103 expression. (ox.ac.uk)
NKG2A19
CD94/NKG2A complexes are also found on a subset of CD8+ T cells. (rndsystems.com)
Most human NK cells express inhibitory receptors specific for HLA class I molecules including the clonally distributed killer immunoglobulin-like receptors (KIRs), that are able to distinguish among different HLA-A, -B and -C allotypes, [ 2 ] and the CD94/NKG2A heterodimer that is specific for HLA-E ( Table 1 ). (medscape.com)
Interestingly, HCMV encodes a glycoprotein UL40, which increases the expression of HLA-E on the surface of virus-infected cells and thus their resistance to killing by CD94/NKG2A + NK cells. (medscape.com)
DNA sequence encoding Human NKG2A&CD94 including a N-His-Avi tag was expressed in HEK293 Cells. (reprokine.com)
Recombinant Human NKG2A&CD94 is a protein consisting of amino acid residues [Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)] ,The protein has a predicted MW of 17.9 kDa (CD94)&18.3 kDa (NKG2A). (reprokine.com)
Biotinylated Human NKG2A&CD94 on Tris-Bis PAGE under reduced condition. (reprokine.com)
The purity of Biotinylated Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC. (reprokine.com)
Dose response curve for Biotinylated Human NKG2A&CD94, His Tag with the EC50 of 0.11μg/ml determined by ELISA. (reprokine.com)
CD94/NKG2A and killer immunoglobulin-like receptors (KIR)) that recognize MHC class I molecules. (hindawi.com)
Target cell pre-activation significantly enhanced CD8+ T granule-mediated cell killing of CD4+ targets, and was restricted by HLA-E. During exacerbations, killer-inhibitory receptor CD94/NKG2A expression was significantly higher in CD8+ TCCs, limiting their cytotoxic activity. (nih.gov)
Moreover, IL-15 and IFN-gamma significantly increased CD94 and NKG2A expression. (nih.gov)
These data provide evidence that CD94/NKG2A receptors play an important role in regulating T cell activity during the course of MS. (nih.gov)
The result of 0.2 ng/ml IL12 in the expression from the CD94, NKG2C and NKG2A receptor stores was analyzed by stream cytometry in gated Compact disc56+ cells. (sciencepop.org)
In contrast CD56bright NK cells mostly lack the expression of these receptors but reveal to a high percentage expression of the inhibitory receptor CD94/NKG2A. (sciencepop.org)
Mouse Cd94 and Nkg2a mRNA were detectable in splenocytes of wild-type (+/+) mice. (biocytogen.jp)
Human CD94 and NKG2A mRNA were detectable only in the homozygous B-hCD94/hNKG2A, but not in +/+ mice. (biocytogen.jp)
Splenocytes were collected from WT and homozygous B-hCD94/hNKG2A (H/H) mice, and analyzed by flow cytometry with species-specific CD94 or NKG2A antibody. (biocytogen.jp)
Mouse CD94 and NKG2A were detectable in WT mice. (biocytogen.jp)
Human CD94 and NKG2A were exclusively detectable in homozygous B-hCD94/hNKG2A but not WT mice. (biocytogen.jp)
NKG22
The type-II transmembrane glycoprotein CD94 covalently associates with C-type lectins of the NKG2 family to yield heterodimers important in NK cell recognition of class I MHC molecules. (rndsystems.com)
KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. (nih.gov)
Amino acids1
There are two alternatively spliced variants of CD94 that differ by 31 amino acids encoded by exon 2. (rndsystems.com)
Human2
5. Expansion of CD94/NKG2C+ NK cells in response to human cytomegalovirus-infected fibroblasts. (nih.gov)
Human CD94 were exclusively detectable in activated CD8+ T cells of homozygous B-hCD94/hNKG2A mice after treated with mCD3e 24h. (biocytogen.jp)