Cd70 cell surface. Medical search. Frequent questions

Several antigens have been used as targets for CAR-T cell therapy against MM, including B cell maturation antigen (BCMA), CD19, CD138, signaling lymphocytic activation molecule 7 (SLAM7), and immunoglobulin light chains. (biomedcentral.com)

We now understand that adaptive immunity is the purview of a second set of leukocytes, T and B lymphocytes, which are responsible not only for antibody production but also for the generation of T cells that can directly identify and kill host cells infected by pathogens (e.g., viruses). (aacrjournals.org)
These cells are involved in antibody response during an infection or vaccination. (wikipedia.org)
Recent functional studies indicate a further subdivision of labor assigning B1a cells as the producers of natural serum antibody (7). (wikipedia.org)
In contrast, B1b cells appear to be the primary source of dynamic T cell independent (TI) antibody production and long-term protection after bacterial infection such as Borrelia hermsii and Streptococcus pneumoniae. (wikipedia.org)
B1b cells are the most common B cells involved in antibody response during an infection or vaccination. (wikipedia.org)
Customarily prepared antibody-drug conjugate (ADC) products refer to the conjugation of therapeutic antibodies against cancer surface antigens with different drug-linker complexes that serve the course of ADC development and evaluation. (adcreview.com)
Cell Proliferation Induced by CD27 Ligand/TNFSF7 and Neutralization by Mouse CD27 Ligand/TNFSF7 Antibody. (bio-techne.com)
In the presence of sub-optimal amounts of Mouse CD3e Monoclonal Antibody (Catalog # MAB484 ), Recom-binant Mouse CD27 Ligand/ TNFSF7 (Catalog # 783-CL ) stimulates proliferation in mouse splenic T cells in a dose-dependent manner (orange line). (bio-techne.com)
Surprisingly, we previously found that mice deficient in iNKT cells developed spontaneous CD8 + T cells responses partially effective at inhibiting metastases in mice bearing the 4T1 mammary carcinoma, and showed a markedly improved response to treatment with local radiotherapy and anti-CTLA-4 antibody compared to wild type (WT) mice. (biomedcentral.com)
The effects of antibody-mediated blockade of CD1d on DC number and phenotype, priming of anti-tumor T cells, and tumor response to treatment with local radiotherapy and anti-CTLA-4 antibody were evaluated. (biomedcentral.com)
To determine if the improved response to treatment in the absence of iNKT cells was independent from the immunotherapy employed, 4T1-tumor bearing WT and iNKT −/− mice were treated with local radiotherapy in combination with antibody-mediated CD137 co-stimulation. (biomedcentral.com)
This COVID-19 GeoMx-formatted Antibody is run with the 20-plex GeoMx Immune Cell Profiling Core (plus controls) with readout on the nCounter Analysis System. (bioinnovations.in)
2018). In another experiment, treatment with an antibody directed against the costimulatory molecule GITR in CD34+ HSC-engrafted NSG TM mice co-engrafted with the human melanoma cell line SK-MEL-5 suppressed tumor growth and reduced intratumoral T regulatory cell number concurrent with increased activation of CD8+ T cells, mirroring what had been observed in syngeneic model systems (Mahne et al. (jax.org)
Mouse Anti-Canine Distemper Virus Surface Envelope Antibody. (eccscotland.com)
Assay cell lines are used in a variety of applications, including gene functional studies, binding/blocking screening assay, and cross-species binding screening assay during antibody development. (genscriptprobio.com)
GenScript ProBio has successfully constructed more than 500 assay cell lines, of which more than 100 can be used for antibody development, such as PD-1, PD-L1, Tim3, CTLA4, Lag3, Siglec15 as well as GPCR cell lines. (genscriptprobio.com)
Anchoring secreted proteins on a cellular surface is a common way to enable antibody screening. (genscriptprobio.com)

CD70 is expressed on activated T, B and NK cells, activated plasmacytoid dendritic cells (pDCs), and chronic B cell lymphocytic leukemia and large B cell lymphomas. (biolegend.com)
Additional reported applications for the relevant formats include: blocking of plasmacytoid dendritic cell induced B cell proliferation and Ig secretion 1 . (biolegend.com)
Dendritic cells (DC) are responsible for initiating all antigen-specific immune responses. (aacrjournals.org)
Upon completion of this activity, the participant should understand the critical roles of dendritic cells in guiding host immune responses, and the details of how they mature, process, and present antigens. (aacrjournals.org)
Understanding the paradoxical importance of dendritic cells in immunity and tolerance will inform the development of successful cancer immunotherapies. (aacrjournals.org)
It has long been appreciated that the innate and adaptive arms of the immune system must be coordinated, but it was not until the discovery of dendritic cells (DC) by Ralph Steinman at The Rockefeller University in the early 1980s that we truly understood conceptually how the immune system functions as a coherent unit. (aacrjournals.org)
This results in extramedullary hematopoiesis and activating dendritic cell. (wikipedia.org)
Targeting resistance to radiation-immunotherapy in cold HNSCCs by modulating the Treg-dendritic cell axis. (cuanschutz.edu)
PD-L1 Reverse Signaling in Dermal Dendritic Cells Promotes Dendritic Cell Migration Required for Skin Immunity. (cuanschutz.edu)
Milk fat globule - epidermal growth factor 8 (MFG-E8) is a glycoprotein secreted by activated macrophages and immature dendritic cells. (soton.ac.uk)
receptors and conferred enhanced phagocytosis by both macrophages and immature dendritic cells (DN-MFG-E8-Fc) in a dose-dependent manner. (soton.ac.uk)
Cross-presentation of cell-associated antigen by bone marrow-derived dendritic cells (BMDCs) was determined by CD8+ T cell proliferation assays in vitro and in vivo. (soton.ac.uk)
Although CD27L expression is not detected on human dendritic cells (DC), membrane expression on mature mouse DCs has been reported. (bio-techne.com)
NK cells contribute to cancer immune surveillance not only by their direct natural cytotoxicity which is triggered rapidly upon stimulation through germline-encoded cell surface receptors, but also by modulating T-cell mediated antitumor immune responses through maintaining the quality of dendritic cells and enhancing the presentation of tumor antigens. (frontiersin.org)
To understand the mechanisms of the immunosuppressive function of iNKT cells, dendritic cells (DCs) were analyzed by immunohistochemistry and flow cytometry in WT and iNKT-deficient (iNKT −/− ) mice. (biomedcentral.com)
Both antibodies recognized human Langerhans cells, previously shown to express RANK, while dermal dendritic cells were poorly labeled. (cnrs.fr)
It showed remarkable cell promiscuity, being active in human skin dendritic cells, hepatoma cell lines and Vero cells, and was active against all four DENV serotypes, with an IC50 in the low micromolar range. (cnrs.fr)

In surface Ig- and CD40-stimulated B cells, this cytokine along with CD70 has been shown to provide CD28-independent costimulatory signals to T cells. (elabscience.com)
Activated γδ T cells exhibit strong cytotoxic activity and cytokine secretion functions and are effective antitumor lymphocytes with simple and direct recognition modes and rapid responses. (explorationpub.com)
First, γδ T cells exposed to complicated cytokine networks are potentially affected by multiple inhibitory mechanisms. (explorationpub.com)
In addition, we showed that pretreatment of iPS-fibro with proinflammatory cytokine IFNγ restored the ligand imbalance, thereby reducing the degranulation and cytotoxicity of NK-cells. (biomedcentral.com)
Peak ferritin levels correlated with UCART22 cell expansion and cytokine release syndrome (CRS). (ricentral.com)
The name TGF-β derives from the transforming activity of the cytokine, which induces anchorage-independent growth when administered to cells together with EGF or without the need to add EGF, depending on the cell system. (antibodies-online.cn)

What differentiates B1 cells from other B cells is the variable existence of CD5, CD86, IgM and IgD. (wikipedia.org)
Apoptotic cells treated with DN-MFG-E8-Fc protein resulted in upregulation of costimulatory molecules, CD86 and CD70, when BMDCs were deficient for the inhibitory Fc?R, Fc?RIIb. (soton.ac.uk)
Oncoselective transduction of CD80 and CD86 in tumor cell lines using an autonomous recombinant parvovirus. (ac.be)
Based on their oncotropic properties, we used autonomous recombinant parvoviruses to transduce the genes coding for the constimulatory molecules CD80 (B7-1) or CD86 (B7-2) specifically into tumor cells without transducing normal cells. (ac.be)
MATERIALS AND METHODS: After infection of tumor cells by these viruses, surface expression of CD80 and CD86 molecules was assessed by FACS and enhancement of immunostimulatory properties was assessed in alloreactions with G-10 purified T cells. (ac.be)

CB-010 is designed as an off-the-shelf allogeneic anti-CD19 CAR-T cell therapy, and data released in May revealed a 100% overall response rate (ORR, 5 of 5 patients) and an 80% complete response rate (CR, 4 of 5 patients), following a single dose at the initial dose level in patients with aggressive r/r B-NHL. (crisprmedicinenews.com)
Specfically, a CD19-specific CAR is inserted into the TRAC gene, which encodes the T cell receptor alpha constant. (crisprmedicinenews.com)
Chimeric antigen receptor T (CART) cell therapy targeting the B cell specific differentiation antigen CD19 has shown clinical efficacy in a subset of relapsed/refractory (r/r) diffuse large B cell lymphoma (DLBCL) patients. (frontiersin.org)
In vitro , CD3 + CD8 + CD27 - CD28 - compared to CD3 + CD8 + CD27 + CD28 + CART cells displayed similar CD19 + target cell-specific cytotoxicity, but were hypoproliferative and produced less cytotoxic cytokines (IFN-γ and TNF-α). (frontiersin.org)
CD3 + CD8 + T cells outperformed CD3 + CD4 + T cells 3- to 6-fold in terms of their ability to kill CD19 + target cells. (frontiersin.org)
Fig. 1: CAR19-mediated trogocytosis in NK cells cocultured with CD19 + tumor targets. (nature.com)
B1 cells express high levels of CD19, IgM and CD11b, and can be further divided into CD5 + B1a and CD5 - B1b subsets. (oncotarget.com)
Our clinical data presented for UCART22 at the European Hematology Association (EHA) were positive for patients with r/r B-ALL who have failed multiple lines of treatment including multi-agent chemoimmunotherapy, CD19 directed CAR T-cell therapy, and allogeneic stem cell transplant. (ricentral.com)

Adoptive transfer of expanded natural killer cells inhibited the growth of established OPM2 and high-risk primary myeloma tumors grown in the murine model. (haematologica.org)
Next, the T cells are engineered to express CARs, which recognise certain cell-surface proteins that are expressed in hematologic or solid tumors (i.e CD70 molecule). (crisprmedicinenews.com)
While GB tumors are frequently infiltrated by natural killer (NK) cells, these are actively suppressed by the GB cells and the GB tumor microenvironment. (frontiersin.org)
Here we describe a novel mechanism of tumor immune escape mediated by iNKT cells that limit priming of anti-tumor T cells by controlling DC in tumors and draining lymph nodes. (biomedcentral.com)
Similarly mice engrafted with a melanoma cell line and treated with the vemurafenib analogue PLX4720 also exhibited increased T cell infiltration in tumors and responsiveness to antigens (18 22 The anti-tumor effects of PLX4720 in this engraftment model was particularly dependent on CD8 T cells and could be enhanced by CD137 agonistic mAb treatment suggesting that BrafV600E inhibitors can sensitize tumors to certain immunotherapies (22). (cancer-pictures.org)
In contrast another study concluded that PLX4720 decreased T cell infiltration in the tumors and were unable to enhance anti-tumor responses in conjunction with CTLA4 blockade (23). (cancer-pictures.org)
For example, co-engrafting human tumors with human immune cells into the NSG TM (NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ, JAX Stock# 005557 ) and its variant strains creates a powerful system to interrogate the immunomodulatory effects of human-specific experimental treatments in a preclinical setting. (jax.org)

CD70 costimulates T cell proliferation and differentiation. (biolegend.com)
It plays a role in the pDC-induced B cell differentiation. (biolegend.com)
Chronic Liver Disease in Humans Causes Expansion and Differentiation of Liver Lymphatic Endothelial Cells. (cuanschutz.edu)
Moreover, BCMA plays an essential role in regulating B cell maturation and differentiation into plasma cells. (biomedcentral.com)
Explore our series of recombinant neural factors to support the culture and differentiation of nerve cells. (acrobiosystems.com)
CD27 binds to CD70, and plays a role in costimulation of T cell activation and regulation of B cell differentiation and proliferation. (biolegend.com)
1989. Leucocyte Typing IV: White Cell Differentiation Antigens. (biolegend.com)
1995. Leucocyte Typing V: White Cell Differentiation Antigens. (biolegend.com)
In addition, cytokines are part of stem cell differentiation, vaccine efficacy and allograft rejection. (antibodies-online.cn)

Because of its ligands, cd70 or cd76 (b5.1 or b7.1). (themauimiracle.org)
Transcriptome analysis revealed a significant imbalance of ligands for activating and inhibitory NK-cell receptors in iPS-fibro. (biomedcentral.com)
Thus, the balance of ligands for NK-cell receptors should be considered prior to iPSC-based cell therapies. (biomedcentral.com)

Neural factors are a class of protein molecules with neurotrophic activity that can promote the survival and regeneration of nerve cells. (acrobiosystems.com)
RESULTS: Infection of normal and transformed cells with recombinant MVM- B7-1 or B7-2 viruses leads to expression of costimulatory molecules only by tumor cells and confers on them the capacity to sensitize naive T cells in vitro. (ac.be)
CONCLUSION: This approach should ultimately lead to selective expression of costimulatory molecules in tumor tissues in vivo without affecting normal cells. (ac.be)
iPSC-derivatives with B2M knockout did not cause an additional increase in NK-cell activation, although they were devoid of HLA-I, the major inhibitory molecules for NK-cells. (biomedcentral.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
The navigators target specific tumor cells and the small molecules enter tumor cells to kill them. (genemedi.com)

Measured by its ability to neutralize CD27 Ligand/TNFSF7-induced proliferation in mouse splenic T cells. (bio-techne.com)
CD27 ligand (CD27L), also named CD70, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7 (1, 2). (bio-techne.com)

Taking advantage of the diverse γδ TCR repertoire or other ligand-receptor interactions, γδ T cells can recognize a broad spectrum of tumor-associated antigens (TAAs) in a major histocompatibility complex (MHC)-independent manner, thereby activating downstream pleiotropic effects. (explorationpub.com)

However, this finding has triggered further interest in utilising tumour-specific MHC-II to harness sustained anti-tumour immunity through the activation of CD4 + T helper cells. (portlandpress.com)
Immunotherapy utilizing T cell immunity has become a new treatment to eliminate cancer cells. (biomedcentral.com)
OX-40L is an important costimulatory molecule that plays a crucial role in the regulation of T-cell-mediated immunity. (elabscience.com)
and interleukin-10 (IL-10) that dampen the initiation of cytotoxic T cell (CTL) immunity and render T cells tolerant. (soton.ac.uk)
Invariant natural killer T (iNKT) cells are CD1d-restricted T cells, which respond rapidly to antigen recognition and promote development of anti-tumor immunity in many tumor models. (biomedcentral.com)
Natural killer T (NKT) cells comprise a subset of lymphocytes originating from a distinct developmental lineage [ 1 ] which bridge innate and adaptive immunity and modulate immune responses in autoimmunity, malignancies and infections [ 2 ]. (biomedcentral.com)

Three selected candidate biomarkers (CD59, CD70, and CD74) mediate the evasion of the immune response. (genengnews.com)
CD5-CD72 is thought to mediate B cell-B cell interaction. (wikipedia.org)
This protein and its receptor are reported to directly mediate adhesion of activated T cells to vascular endothelial cells. (elabscience.com)
γδ T cells recruited into the tumor microenvironment can act as effector cells to mediate cancer immune surveillance. (explorationpub.com)
5) Lysosomes can also fuse with the plasma membrane to mediate membrane repair or discharge contents outside the cell, such as cathepsins or immune factors. (biomedcentral.com)

The transferred, expanded natural killer cells proliferated in vivo in an interleukin-2 dose-dependent fashion, persisted up to 4 weeks, were readily detectable in the human bone, inhibited myeloma growth and protected bone from myeloma-induced osteolysis. (haematologica.org)
receptors (Fc?Rs) reduced their ability to induce CD8+ T cell proliferation in vivo, suggesting the blockade of a mechanism which is intrinsic for DC maturation or migration. (soton.ac.uk)
Nevertheless, ex vivo activation with cytokines can restore cytolytic activity of NK cells against GB, indicating that NK cells have potential for adoptive immunotherapy of GB if potent cytotoxicity can be maintained in vivo . (frontiersin.org)
Here, we discuss effects of the GB tumor microenvironment on NK-cell functionality, summarize early treatment attempts with ex vivo activated NK cells, and describe relevant CAR target antigens validated with CAR-T cells. (frontiersin.org)
In vivo R12-31 agonist activity was demonstrated by its ability to induce the formation of intestinal villous microfold cells in mice. (cnrs.fr)
Antigens targeted to DCs via the C-type lectin Langerin/CD207 are cross-presented to CD8(+) T cells in vivo. (cnrs.fr)
Fig. 3: Impact of TROG-antigen acquisition on CAR-NK cell phenotype and function in vivo. (nature.com)
Fig. 6: AI-CAR-expressing NK cells showed superior in vivo antitumor activity. (nature.com)
These cells can be activated ex vivo to exhibit a potent antitumor effector function. (biomedcentral.com)
The study and/or manipulation of administered cells can potentially result in identification of the exact cell subpopulations and effector functions required for cancer regression in vivo. (biomedcentral.com)
In culture, RANKL-expressing B1a cells significantly promoted the expansion of osteoclasts derived from bone marrow cells, which were in accord with the in vivo findings of increased osteoclastogenesis in CIA mice transferred with B1a cells. (oncotarget.com)
While many methods have been developed to screen and evaluate the efficacy of immune cell‑mediated killing, there is a need for a more robust in vitro assay to accurately predict the in vivo behavior of therapies. (technologynetworks.com)
While the data provided by these assays help piece together an understanding of different facets of immune cell‑mediated killing, it is important to note that the parameters being reported often do not correlate with target cell killing efficacy in vivo. (technologynetworks.com)

There are two types of B1 cells subsets, B1a cells and B1b cells. (wikipedia.org)
It is of great significance to gain insight into the classification of γδ T cell subsets. (explorationpub.com)
CD27L interacts with CD27, a member of the TNF receptor superfamily that is expressed on natural killer (NK) cells and subsets of T and B cells (2, 4, 5). (bio-techne.com)

Multiple myeloma (MM) is a hematological malignancy characterized by the proliferation of transformed monoclonal plasma cells in the bone marrow (BM) [ 1 ]. (biomedcentral.com)
Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation (2). (bio-techne.com)
In addition, previous studies have shown that lysosomes may play important roles in cancer development and progression through the abovementioned biological processes and that the functional status and spatial distribution of lysosomes are closely related to cancer cell proliferation, energy metabolism, invasion and metastasis, immune escape and tumor-associated angiogenesis. (biomedcentral.com)
P is the proliferation score, which is the average gene expression profile of genes associated with cell-cycle progression and T is the tumor stage. (bioinnovations.in)
In this study, we found that peritoneal B1a cells underwent proliferation and migrated to the inflamed joint tissue with upregulated RANKL expression during collagen-induced arthritis (CIA) development in mice. (oncotarget.com)

The high diagnostic potential of peptides and proteins from cells, tissues, and biological fluids is unquestionable. (genengnews.com)
Analysis of immunophenotypically defined cell populations markedly increased specificity of liquid chromatography-tandem mass spectroscopy (LC-MS/MS). Over 375 differentially expressed proteins were analyzed by Ingenuity Pathway Analysis (Ingenuity Systems). (genengnews.com)
Here, two recombinant dominant-negative MFG-E8 proteins were generated: one which is shown to inhibit PS-dependent apoptotic cell uptake by macrophages by over 40% (DN-MFG-E8), and a second which re-directed apoptotic cells through Fc? (soton.ac.uk)
Users can add up to six 10-plex modules including the Immune Activation Status, Immune Cell Typing, and/or Cell Death modules to more deeply profile proteins involved in T cell activation and cell death. (bioinnovations.in)

Trogocytosis is an active process that transfers surface material from targeted to effector cells. (nature.com)
Endogenous biomolecules (such as lactate dehydrogenase) or previously added exogenous labels (such as the radioisotope 51Cr) that leak into the media are then measured as an indirect readout of the damage caused by effector cells. (technologynetworks.com)

Cancer immunotherapies can be divided into four major categories: - Cytokines/immunomodulation agents - Monoclonal antibodies - Cell‑based therapies - Oncolytic viruses Though monoclonal antibodies currently represent the largest class of commercialized cancer immunotherapies, cell‑based therapies are rapidly making headway. (technologynetworks.com)

Expanded natural killer cells killed both allogeneic and autologous primary myeloma cells avidly via a perforin-mediated mechanism in which the activating receptor NKG2D, natural cytotoxicity receptors, and DNAX-accessory molecule-1 played a central role. (haematologica.org)
B1 cells express IgM in greater quantities than IgG and its receptors show polyspecificity, meaning that they have low affinities for many different antigens. (wikipedia.org)
Furthermore, similar to T cells, specific recognition and elimination of cancer cells by NK cells can be markedly enhanced through expression of chimeric antigen receptors (CARs), which provides an opportunity to generate NK-cell therapeutics of defined specificity for cancer immunotherapy. (frontiersin.org)
Although initially identified by co-expression of conventional αβ T-cell receptors (TCR) and markers typically associated with natural killer (NK) cells [ 3 ], NKT are currently distinguished on the basis of CD1d restriction as well as specific usage of TCRα chains [ 4 ]. (biomedcentral.com)
CD70 CAR-T cells were modified to express receptors for IL-8 to enhance intratumoral T cell migration as expression of this chemokine is increased in tumor tissue and can be stimulated by radiation. (jax.org)

We tested whether natural killer cells expanded by co-culture with K562 cells transfected with 41BBL and membrane-bound interleukin-15 could kill myeloma cells with a high-risk gene expression profile in vitro and in a unique model which recapitulates human myeloma. (haematologica.org)

CD70, also known as CD27L, is a 50 kD type II transmembrane glycoprotein and member of the tumor necrosis factor superfamily. (biolegend.com)
BCMA, a member of the tumor necrosis factor (TNF) superfamily, is exclusively expressed in a subpopulation of B cells, normal plasma cells, and malignant plasma cells. (biomedcentral.com)
It has been reported that CD70, a B cell costimulatory molecule encoded by the gene TNFSF7 (tumor necrosis factor ligand superfamily member 7), is overexpressed in CD4 + T cells from patients with SLE due to the demethylation of its promoter. (biomedcentral.com)
CD70, a B cell costimulatory molecule encoded by the TNFSF7 (tumor necrosis factor ligand superfamily member 7) gene [ 21 ], is typically expressed by activated CD4 + T and CD8 + T cells and early B cell progenitors. (biomedcentral.com)

On May 12th 2022, Caribou Biosciences announced positive initial clinical trial data for its gene-edited CAR-T cell therapy candidate CB-010, which is being evaluated in the ANTLER Phase 1 trial for the treatment of relapsed/refractory B cell non-Hodgkin lymphoma (r/r B-NHL). (crisprmedicinenews.com)
The PD-1 gene is also deleted in these cells. (crisprmedicinenews.com)
This gene encodes the PD-1 protein that functions as a safety switch on T cells that cancer cells turn on to protect themselves from T cell-mediated immune responses. (crisprmedicinenews.com)
B1 cells generate diversity mainly via recombinatorial recombination (there is a preferential recombination between D-proximal VH gene segments). (wikipedia.org)
These cells are isolated in a manufacturing facility, engineered to express CARs to recognise and destroy cancer cells, and modified via gene editing to limit autoimmune response when given to a patient. (crisprmedicinenews.com)
A T cell receptor gene is knocked out to avoid GVHD. (crisprmedicinenews.com)
Our results indicated that downregulation of MBD4 contributed to overexpression and hypomethylation of the CD70 gene in SLE CD4 + T cells. (biomedcentral.com)
However, some reports have claimed recently that aberrant gene expression followed by proteome alterations and neoantigen formation can result in iPSCs recognition by autologous T-cells. (biomedcentral.com)
NEW YORK, Aug. 03, 2023 (GLOBE NEWSWIRE) -- Cellectis (the "Company") (Euronext Growth: ALCLS - NASDAQ: CLLS), a clinical-stage biotechnology company using its pioneering gene-editing platform to develop life-saving cell and gene therapies, today provided business updates and preliminary financial results for the six-month period ending June 30, 2023. (ricentral.com)
This quarter, Cellectis presented an encore of the clinical data on the AMELI-01 clinical trial at the American Society of Gene and Cell Therapy (ASGCT) 2023 annual meeting. (ricentral.com)
Cellectis' innovation team also presented preclinical data on a gene editing process to develop a bona fide HBB gene correction of sickle cell mutation, and a comprehensive analysis to better design efficient TALE Base Editors (TALE-BE) at the International Society for Cell and Gene Therapy (ISCT) 2023 annual meeting. (ricentral.com)
Despite an unprecedented challenging market environment for cell and gene therapy companies, Cellectis remains focused in its mission to develop innovative cancer therapy product candidates. (ricentral.com)
GenScript ProBio possesses a series of assay cell line construction technologies and is extensively experienced in mammalian cell culture and gene-editing, which enables the construction of a variety of assay cell lines and functional reporter assay cell line through CRISPR, Tet-On technology, lentivirus infection, electroporation, and lipo-transfection, thus shortening your project turnaround time. (genscriptprobio.com)
Service package including gene synthesis, vector construction, lentivirus packaging, and host cell characterization to ensure the delivery of cell lines with stable expression of genes inserted. (genscriptprobio.com)

Chimeric antigen receptor (CAR) T cell therapy has emerged as a novel immunotherapy which modifies T cells with CAR, an artificial fusion protein that incorporates an extracellular antigen recognition domain, a transmembrane domain, and an intracellular domain including costimulation and signaling components [ 4 , 5 ]. (biomedcentral.com)
We then outline preclinical approaches that employ CAR-NK cells for GB immunotherapy, and give an overview on the ongoing clinical development of ErbB2 (HER2)-specific CAR-NK cells currently applied in a phase I clinical trial in glioblastoma patients. (frontiersin.org)
Fig. 4: A lower level of CAR-mediated TROG-antigen expression was associated with improved clinical response to CAR-NK cell-based immunotherapy. (nature.com)
This has led to the need for new immunotherapy-based approaches, including adoptive cell transfer (ACT), therapeutic vaccines, and novel immune checkpoint inhibitors. (biomedcentral.com)
The use of cell therapies, drivers of immune response, and trends in immunotherapy were the focus of the Immunotherapy Bridge (November 30th-December 1st, 2022), organized by the Fondazione Melanoma Onlus, Naples, Italy, in collaboration with the Society for Immunotherapy of Cancer. (biomedcentral.com)
This has resulted in the development of additional immunotherapy-based approaches, including adoptive cell transfer (ACT), therapeutic vaccines, and inhibitors of other immune checkpoints. (biomedcentral.com)
The use of cell therapies, drivers of immune response, and trends in immunotherapy were the focus of the Immunotherapy Bridge (November 30th-December 1st, 2022). (biomedcentral.com)
4 xCELLigence real-time cell analysis 6 Diverse cancer immunotherapy applications 6 How does the xCELLigence real-time cell analysis assay work? (technologynetworks.com)

Chronic CD27-CD70 costimulation promotes type 1-specific polarization of effector Tregs. (ac.be)

This phenomenon could be offset by a dual-CAR system incorporating both an activating CAR against the cognate tumor antigen and an NK self-recognizing inhibitory CAR that transferred a 'don't kill me' signal to NK cells upon engagement with their TROG + siblings. (nature.com)
The lack of inhibitory signals might be due to insufficient maturity of cells differentiated from iPSCs. (biomedcentral.com)
Obviously, compared with the blank group, ADC had a significant inhibitory effect on the growth of 293 cells. (genemedi.com)

The cellular or innate immune system was first described by the pathologist Elie Metchnikoff who recognized the existence of cells in the blood and tissues whose role was to identify, capture, and destroy invading pathogens. (aacrjournals.org)
Our team was the first to conduct an in-depth molecular comparison of cancer and normal tissues for the presence of cancer stem cells," says David M. Lubman, Ph.D., professor of surgery at the University of Michigan, Ann Arbor. (genengnews.com)
In adult mice, B1 cells constitute a minor fraction of the spleen and secondary lymphoid tissues but are enriched in the pleural and peritoneal cavities. (wikipedia.org)
BCMA is not present in other hematological cells like hematopoietic stem cells or other tissues. (biomedcentral.com)

We aimed to investigate the expression patterns of MBD4 (methyl-CpG binding domain protein 4) in CD4 + T cells and its contribution to the pathogenesis of SLE by increasing CD70 expression through epigenetic regulation. (biomedcentral.com)
Using a cell apoptosis assay based on stimulation of a RANK:Fas fusion protein, and a cellular NF-kappaB signaling assay, we showed that R12-31 was agonist for both species. (cnrs.fr)
A common problem when developing assay cell lines is that the protein expression level is relatively low but mRNA level is high. (genscriptprobio.com)
By generating assay cells expressing different protein isoforms, antibodies' specificity can be validated using FACS binding assay. (genscriptprobio.com)

In this paper, we investigated the role of Immunoglobulin G (IgG) autoantibodies directed against epithelial cells, as potential initiators and propagators of pathological keratocyte activation and the ensuing SSc fibrotic cascade. (bvsalud.org)

Human myeloma cell line, U266, was stained with CD70 (clone 113-16) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram). (biolegend.com)
These mice are devoid of endogenous natural killer and T-cell activity and were used to determine whether adoptively transferred expanded natural killer cells could inhibit myeloma growth and myeloma-associated bone destruction. (haematologica.org)
Results Natural killer cells from healthy donors and myeloma patients expanded a median of 804- and 351-fold, respectively, without significant T-cell expansion. (haematologica.org)
Multiple myeloma (MM) is a malignant plasma cell disorder with debilitating symptoms related to anemia, immunosuppression, bone destruction, and renal failure. (haematologica.org)

B1 cells are a sub-class of B cell lymphocytes that are involved in the humoral immune response. (wikipedia.org)
Using the cells obtained from two patients, we analyzed the activation of autologous and allogeneic T-lymphocytes and NK-cells co-cultured with target cells. (biomedcentral.com)

There is an unmet need to develop novel therapies for refractory/relapsed MM. In the past few years, chimeric antigen receptor (CAR)-modified T cell therapy for MM has shown promising efficacy in preclinical and clinical studies. (biomedcentral.com)
This class of personalized therapies involves collecting immune cells from an individual, engineering them to recognize and kill cancer cells, before culturing, and reintroducing them into the same individual. (technologynetworks.com)

Humanized NSG TM have also proven to be a valuable platform for assessment of anti-tumor T cell activity using both chimeric antigen receptor T cells (CAR-T) and bi-specific T cell engagers (BiTE). (jax.org)

Our previous studies have shown that DNA hypomethylation contributes to the auto-reactivity of T cells by increasing the expression of some autoimmune genes, such as CD70, CD11a, and CD40L in CD4 + T cells of SLE patients. (biomedcentral.com)
associated with a relative increase in CD40L and IFNγ expression on intratumoral CD4+ TILs and a reduced accumulation of Tregs and CD11b+/Gr-1+ myeloid cells. (cancer-pictures.org)

Their expression in tumour cells is modulated by a complex interplay of genomic, transcriptomic and post translational factors involving multiple intracellular antigen processing pathways. (portlandpress.com)
Ongoing research investigates mechanisms invoked by cancer cells to abrogate MHC-I expression and attenuate anti-tumour CD8 + cytotoxic T cell response. (portlandpress.com)
This review provides a comprehensive overview of physiologically conserved or novel mechanisms utilised by tumour cells to reduce MHC-I or MHC-II expression. (portlandpress.com)
Dose escalation of CTX130 was performed in eligible adults who had at least 10% expression of CD70. (crisprmedicinenews.com)
Our results showed that the expression of MBD4 was significantly decreased in CD4 + T cells from SLE patients. (biomedcentral.com)
We verified that transfection of MBD4 siRNA into healthy CD4 + T cells upregulated expression of CD70 and decreased the methylation level of the CD70 promoter. (biomedcentral.com)
Overexpression of MBD4 inhibited CD70 expression and enhanced the DNA methylation level of CD70 in CD4 + T cells of SLE patients. (biomedcentral.com)
In this study, we compared the expression level of MBD4 in CD4 + T cells between SLE patients and healthy subjects and investigated whether MBD4 was involved in regulating CD70 expression and methylation status. (biomedcentral.com)
CD27L cell surface expression is induced by antigen receptor activation in B cells and at low levels in mouse T cells. (bio-techne.com)
Fig. 5: Expression of an iCAR by NK cells reduced fratricide and exhaustion induced by aCAR. (nature.com)
Multi-color flow cytometry: Current assays in place include T cell memory phenotyping, T-reg identification and phenotyping, CAR-T identification, quantification and phenotyping, T cell absolute counts, plasma cell PD-L1 and PD-L2 expression. (massgeneral.org)

This ADC is developed by Pfizer for the treatment of adults with relapsed or refractory B-cell precursor acute lymphoblastic leukemia. (adcreview.com)

They are not part of the adaptive immune system, as they have no memory, but otherwise, B1 cells perform many of the same roles as other B cells: making antibodies against antigens and acting as antigen-presenting cells. (wikipedia.org)
B1 cells self-renew and spontaneously secrete IgM and IgG3 serum antibodies. (wikipedia.org)
When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. (bdbiosciences.com)
Although B1 cells have been recognized for their protective functions against viruses, bacteria and parasites by producing natural IgM antibodies [ 3 ], increasing evidence indicates that B1 cells, especially the highly polyspecific B1a cells, play a role in autoimmune pathogenesis [ 4 ]. (oncotarget.com)

Dysregulated functions of B1 cells have been implicated in the disease progression of various autoimmune disorders, but it remains largely unclear whether B1 cells are involved in the pathogenesis of autoimmune arthritis. (oncotarget.com)
Together, these results have demonstrated a pathogenic role of B1a cells in the development of autoimmune arthritis through RANKL-mediated osteoclastogenesis. (oncotarget.com)
Recent studies have identified the important contribution of conventional B (B2) cells to the development of autoimmune disease, but our current understanding for the role of B1 cells in autoimmune pathogenesis is still limited [ 1 , 2 ]. (oncotarget.com)

At next step, we did cytotoxity assay to observe the toxic effect of ADC on 293 cells. (genemedi.com)
The most commonly used method for measuring immune cell‑mediated killing is the release assay, where effector cell‑mediated disruption of the target cell membrane results in leakage of its cytoplasmic contents into the culture medium. (technologynetworks.com)

Finally, it retrospectively analyze the activation strategies and clinical relevance of existing γδ T cell adoptive immunotherapies. (explorationpub.com)
Adoptive transfer of B1a cells exacerbated arthritic severity and joint damage while intraperitoneal depletion of B1 cells ameliorated both arthritic symptoms and joint pathology in CIA mice. (oncotarget.com)

This data makes CB-010 the first allogeneic CAR-T cell therapy to achieve 100% ORR. (crisprmedicinenews.com)
CB-010 is the first allogeneic CAR-T cell therapy with a CRISPR-mediated PD-1 deletion to be cleared for a clinical trial. (crisprmedicinenews.com)
UCART22 is an allogeneic CAR T-cell product candidate targeting CD22 and is being evaluated in patients with r/r B-ALL in the BALLI-01 Phase 1/2a clinical study. (ricentral.com)
UCART22 is currently the most advanced allogeneic CAR T-cell product in development for r/r B-ALL. (ricentral.com)

Assessing novel therapeutics that function to modulate the interaction between the human immune system and cancer cells requires advanced preclinical efficacy testing platforms. (jax.org)

Remarkably, depletion of B1a cells by osmotic pressure ameliorates the development of type I diabetes [ 5 ], and reduces renal injury after kidney ischemia or reperfusion [ 9 ], indicating the involvement of B1a cells in disease progression. (oncotarget.com)

These studies indicate preexisting subset differences in B-cell receptor (BCR) specificity and antigen-driven B cell fate that remain important unresolved features of the system. (wikipedia.org)

One is the ability to administer large numbers of highly selected cells with high avidity for tumor antigens that can recognize shared and patient-specific mutated (neo) antigens. (biomedcentral.com)

It was hypothesised that manipulation of this clearance pathway, which is normally employed to prevent autoimmunity, may alleviate immuno-suppression of apoptotic tumour cells and promote the expansion of a tumour antigen-specific T cell response. (soton.ac.uk)

B1 cells are present in low numbers in the lymph nodes and spleen and are instead found predominantly in the peritoneal and pleural cavities. (wikipedia.org)
In mice, B1 cells mainly reside in the peritoneal and pleural cavities, but aberrant B1a cell migration has been found in the pancreas of non-obese diabetic (NOD) mice [ 5 ], and in the kidney, thymus and lung of NZBW F1 mice [ 6 - 8 ]. (oncotarget.com)

Given their central role in controlling the immune response in patients with cancer, DCs are emerging as a critical cell type that must be considered as we come to understand basic cancer immunobiology. (aacrjournals.org)
Type 1 IFN and PD-L1 Coordinate Lymphatic Endothelial Cell Expansion and Contraction during an Inflammatory Immune Response. (cuanschutz.edu)
This study focused on the comparison of autologous and allogeneic immune response to iPSC-derived cells and isogeneic parental somatic cells used for reprogramming. (biomedcentral.com)

Consistently, priming of T cells to a tumor-specific CD8 T cell epitope in mice treated with radiotherapy and anti-CTLA-4 or anti-CD137 was markedly enhanced in iNKT −/− compared to WT mice. (biomedcentral.com)
CD1d blockade restored the number of DC in WT mice, improved T cell priming in draining lymph nodes and significantly enhanced response to treatment. (biomedcentral.com)
They were able to suppress tumor growth in lung, sarcoma, bladder, breast cancer PDX, and a breast cancer cell line when co-engrafted in CD34+ hematopoietic stem cell (HSC) humanized NSG TM mice (Wang et al. (jax.org)
In another study, efficacy of a BiTE was assessed in NSG TM mice engrafted with hematological cancers known to express the intracellular tumor marker WT1, where an epitope of the tumor marker is expressed on the cell surface in the context of the MHC I molecule HLA-A2 (Dao et al. (jax.org)

The candidate is currently being assessed in two Phase 1 clinical trials: the COBALT™-LYM trial for T or B cell cancers and a trial for safety and efficacy of several dose levels in RCC . (crisprmedicinenews.com)
At EHA2022, the company presented positive clinical results from the ongoing COBALT™-LYM trial , which is evaluating the safety and efficacy of CTX130 in adults with relapsed or refractory T or B cell malignancies. (crisprmedicinenews.com)
However, the clinical application of tumor-infiltrating γδ T cells has certain limitations. (explorationpub.com)
Blood cell and serum markers, along with clinical data of DLBCL patients who were scheduled for CART cell therapy were evaluated to search for biomarkers predicting CART cell responsiveness. (frontiersin.org)
Clinical activity of TIL therapy has also been reported in other cancer types, including human papillomavirus-associated cancers, cholangiocarcinoma, non-small-cell lung cancer (NSCLC), and triple-negative breast cancer (TNBC). (biomedcentral.com)
Dozens of transplants generated from pluripotent stem cells are currently in clinical trials. (biomedcentral.com)

CRISPR Therapeutics is developing CTX130 for the treatment of relapsed or refractory T or B cell cancers, including certain lymphomas, as well as renal cell carcinoma (RCC). (crisprmedicinenews.com)

They are exceptionally efficient at antigen presentation and also adept at generating just the right type of T cells in response to a given pathogen. (aacrjournals.org)
A smaller NKT cell subset utilizes a more diverse set of TCR αβ chains and is referred to as type II or non-invariant NKT cells [ 7 ]. (biomedcentral.com)
The long time required to obtain a new iPSCs line, as well as to differentiate it into the desired cell type, is also worthy of emphasis [ 8 ]. (biomedcentral.com)

Renal cell cancer (also called kidney cancer or renal cell adenocarcinoma) is a disease in which malignant (cancer) cells are found in the lining of tubules (very small tubes) in the kidney. (crisprmedicinenews.com)

In addition to blocking anti-tumor immune responses these immunomodulatory cells can also promote tumor growth and metastasis through secretion of angiogenic factors (e.g. (cancer-pictures.org)

The updates include positive data from Caribou Biosciences' ongoing trial for CB-010 in B cell non-Hodgkin lymphoma, positive data for CRISPR Therapeutics' CTX130 in relapsed or refractory T cell malignancies, and long-time positive data for CRISPR Therapeutics' and Vertex Pharamceuticals' jointly-developed exa-cel (CTX001) for beta thalassemia and sickle cell disease. (crisprmedicinenews.com)

These cells, including macrophages and neutrophils, were found to provide a rapid response to virtually all pathogens and to initiate inflammation at sites of local infection. (aacrjournals.org)

Anatomy43

Organisms9

Diseases1

Chemicals and Drugs127

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes49

Disciplines and Occupations1

Information Science3

Signaling lymphocy1

Antibody17

Dendritic17

Cytokine6

CD865

CD198

Tumors7

Differentiation9

Ligands3

Molecules6

CD27 Ligand2

Ligand-receptor1

Immunity6

Mediate5

Vivo13

Subsets3

Proliferation5

Proteins4

Effector cells2

Monoclonal1

Receptors5

Vitro1

Superfamily4

Gene14

Immunotherapy8

Costimulation1

Inhibitory3

Tissues4

Protein4

Epithelial cells1

Myeloma4

Lymphocytes2

Therapies2

Chimeric1

CD40L2

Expression11

Relapsed or refractory B-cell1

Antibodies4

Autoimmune3

Assay2

Adoptive2

Allogeneic CAR-T cell4

Interaction1

Progression1

Specificity1

Tumor antigens1

Antigen-specific1

Peritoneal and pleural cavities2

Immune Response3

Mice4

Clinical6

Renal cell carc1

Type3

Malignant1

Anti-tumor1

Refractory1

Macrophages1