• Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis . (biolegend.com)
  • First, the cells are labeled by Rat PROCR monoclonal antibody conjugated to R-PE and anti-R-PE magnetic beads, the Rat PROCR + cells are positively selected on a magnetic column, are collected for further flow-cytometric analysis. (celltechgen.com)
  • The screening procedure is performed by directly magnetically labeling the cells with the monoclonal antibody against the specific antigen, conjugated to R-PE and Anti-R-PE magnetic beads, then the labeled cells are subsequently retained to a magnetic column, and the target cells are released and collected for further flow cytometric analysis. (celltechgen.com)
  • The R73 monoclonal antibody specifically recognizes the αβ T-cell Receptor (TCR) found on most peripheral T lymphocytes, intestinal intraepithelial lymphocytes, and thymocytes. (bdbiosciences.com)
  • Belimumab (Benlysta) is a monoclonal antibody developed by Human Genome Sciences and GlaxoSmithKline , with significant discovery input by Cambridge Antibody Technology , which specifically recognizes and inhibits the biological activity of B-Lymphocyte stimulator (BLyS) and is in clinical trials for treatment of Systemic lupus erythematosus and other autoimmune diseases. (wikidoc.org)
  • It exists in a complex with MHC class I and II, CD53, CD81, and CD82. (biolegend.com)
  • SDS-PAGE Analysis Purified CD63-Monospecific Mouse Recombinant Monoclonal Antibody (rMX-49.129.5). (neobiotechnologies.com)
  • Analysis of Protein Array containing more than 19,000 full-length human proteins using CD63-Monospecific Mouse Recombinant Monoclonal Antibody (rMX-49.129.5) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (neobiotechnologies.com)
  • Human CD53 is a tetraspan cell surface glycoprotein found on all hemopoetic cells except platelets and red blood cells. (adipogen.com)
  • The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions. (biolegend.com)
  • The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
  • Donor-specific antibodies bind with high affinity to the vascular endothelium of graft and activate complement . (wikidoc.org)
  • Antibody FMC7 ( F linder M edical C entre) appears to recognise a conformational variant of CD20 [10] [11] also known as the FMC7 antigen. (wikidoc.org)
  • CD20 is the target of the monoclonal antibodies rituximab , ocrelizumab , obinutuzumab , ofatumumab , ibritumomab tiuxetan , tositumomab , and ublituximab , which are all active agents in the treatment of all B cell lymphomas , leukemias , and B cell-mediated autoimmune diseases. (wikidoc.org)
  • Obese mice administered anti-B cell CD-20 antibodies, however, did not become less responsive to insulin and as a result did not develop diabetes mellitus or the metabolic syndrome, the posited mechanism being that anti-CD20 antibodies rendered the T cell antibodies dysfunctional and therefore powerless to cause insulin desensitivity by a B cell antibody-modulated autoimmune response. (wikidoc.org)
  • 5) Wash cells to remove unbound primary antibody by adding 1−2 mL of buffer per 10 7 cells and centrifuge at 300×g for 10 minutes. (celltechgen.com)
  • The microtiter plate provided in th is kit has been pre-coated with an antibody specific to VE, During the reaction,VE in the sample or standard competes with a fixed amount of biotin-labeled VE for sites on a pre-coated Monoclonal antibody specific to VE. (biobool.com)
  • An inflammation response that mobilizes both T and B cells results in the creation of antibodies against these cells, causing them to become less responsive to insulin by an as-yet unknown mechanism and promoting hypertension , hypertriglyceridemia , and arteriosclerosis , hallmarks of the metabolic syndrome . (wikidoc.org)
  • Bead release reagent and Stop Buffer can be used to release the cell with antibody-R-PE from magnetic beads. (celltechgen.com)
  • Thus, proper identification of peptides and the corresponding presenting HLA allele in disease phenotypes is important for the design and execution of therapeutic strategies using engineered T-cell receptors or antibodies. (bvsalud.org)
  • Excessive level of BAFF causes abnormally high antibody production, results in systemic lupus erythematosus, rheumatoid arthritis, and many other autoimmune diseases. (wikidoc.org)
  • Here we studied and optimized the spatial resolution for the method using an oncoprotein complex between the antibody drug, trastuzumab (Traz), and its target HER2. (bvsalud.org)
  • The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. (bdbiosciences.com)
  • Here we used phage and yeast display coupled with in cellulo evolution to generate variable heavy (VH) single-domain antibodies against ENPP1 and discovered a VH domain that allosterically inhibited the hydrolysis of cGAMP and adenosine triphosphate (ATP). (bvsalud.org)
  • Based on immunoelectrophoresis, the antibody reacts with the Fc portion of rat IgG heavy chain but not with the Fab portion of rat immunoglobulins. (srgroupchemical.com)
  • 1. Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells. (nih.gov)
  • 2. C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. (nih.gov)
  • 14. Monoclonal antibodies define a cellular antigen involved in HTLV-I infection. (nih.gov)
  • The screening procedure is performed by directly magnetically labeling the cells with the monoclonal antibody against the specific antigen, conjugated to R-PE and Anti-R-PE magnetic beads, then the labeled cells are subsequently retained to a magnetic column, and the target cells are released and collected for further flow cytometric analysis. (celltechgen.com)
  • Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. (bdbiosciences.com)
  • Mouse monoclonal to CD53.COC53 monoclonal reacts CD53, a 32-42 kDa molecule, which is expressed on thymocytes, T cells, B cells, NK cells, monocytes and granulocytes, but is not present on red blood cells, platelets and non-hematopoietic cells. (tech-strategy.org)
  • NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. (bdbiosciences.com)
  • Bead release reagent and Stop Buffer can be used to release the cell with antibody-R-PE from magnetic beads. (celltechgen.com)
  • 5) Wash cells to remove unbound primary antibody by adding 1−2 mL of buffer per 10 7 cells and centrifuge at 300×g for 10 minutes. (celltechgen.com)
  • Supplementary MaterialsS1 Fig: A comparison of histograms generated by K-TOPE when antibodies were added to serum or buffer. (bios-mep.info)
  • Mitnacht R, Tacke M, Hunig T. Expression of cell interaction molecules by immature rat thymocytes during passage through the CD4+8+ compartment: developmental regulation and induction by T cell receptor engagement of CD2, CD5, CD28, CD11a, CD44 and CD53. (bdbiosciences.com)
  • 4. Induction of strong homotypic adhesion in human T cell lines positive with human T-cell leukemia virus type 1 by monoclonal antibodies to MHC class I and beta 2-microglobulin. (nih.gov)
  • 7. Syncytium-inhibiting monoclonal antibodies produced against human T-cell lymphotropic virus type 1-infected cells recognize class II major histocompatibility complex molecules and block by protein crowding. (nih.gov)
  • 11. Characterization of monoclonal antibodies directed against the gp46 of human T-cell leukemia virus type I. (nih.gov)
  • 18. An antigenic structure of the trans-activator protein encoded by human T-cell leukemia virus type-I (HTLV-I), as defined by a panel of monoclonal antibodies. (nih.gov)
  • CD53 cross-linking promotes activation of human B cells and rat macrophages, as well as signal transduction. (tech-strategy.org)
  • Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis . (biolegend.com)