Leukocyte antigenCD45ROAntibodyCD45RLymphocytesAntibodiesDiffer in their extracellular domainsTyrosine phosphataseCommon antigensLymphocyteReceptorT200ExonHI30ProteinBindExtracellularLymphoidExonsTonsilActivationHematopoieticDifferentiationCellsCrucialApoptosisExpressionFunctionsIncludesSurfaceComplex
Leukocyte antigen1
- Antibodies to common leukocyte antigen p220 influence human T cell proliferation by modifying IL 2 receptor expression. (bdbiosciences.com)
CD45RO7
- The CD45RA antigen is a member of the CD45 antigen family that also includes the CD45, CD45RB, and CD45RO antigens. (bdbiosciences.com)
- However, active T cells express shorter isoforms of CD45 (CD45RO, CD45RA) that lack exon B. CLEC10A signalling induces IL-10 production in dendritic cells, in part through increasing intracellular Ca2+ concentration. (wikipedia.org)
- The UCHL1 antibody reacts with the human CD45 isoform known as CD45RO, a protein tyrosine phosphatase of ≥ 220 kDa. (cytekbio.com)
- Human peripheral blood lymphocytes were stained with CD45RA FITC and CD45RO (clone UCHL1) Brilliant Violet 605™ (top) or mouse IgG2a, κ Brilliant Violet 605™ isotype control (bottom). (biolegend.com)
- The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. (biolegend.com)
- The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). (abeomics.com)
- The CD45RO isoform contains none of the alternatively spliced exons and is present on most thymocytes, a subpopulation of resting (memory) T cells within both CD4 and CD8 subsets as well as on monocytes and granulocytes. (adipogen.com)
Antibody13
- The CD45RA antibody, clone L48, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with low-buoyant-density human lymphocytes. (bdbiosciences.com)
- The CD45RA antibody recognizes a 220-kilodalton (kDa) isoform of the leucocyte common antigen (LCA). (bdbiosciences.com)
- This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
- IHC testing of FFPE human tonsil with anti-CD45 antibody (clone SPM570). (nsjbio.com)
- This anti-CD45 antibody is available for research use only. (nsjbio.com)
- This antibody will bind to all CD45 isoforms. (nsjbio.com)
- Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. (nsjbio.com)
- Due to variation in protocol and secondary antibody used, the anti-CD45 antibody may need to be titered for optimal performance. (nsjbio.com)
- Neoplastic cells from a T cell lymphoma were used as the immunogen for the anti-CD45 antibody. (nsjbio.com)
- Anti-CD45 antibody (with azide) can be stored at 2-8oC. (nsjbio.com)
- The mouse monoclonal antibody HI30 recognizes an extracellular epitope on all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. (exbio.cz)
- The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. (biolegend.com)
- The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. (biolegend.com)
CD45R5
- The 2H4 (CD45R) antigen is selectively decreased in multiple sclerosis lesions. (bdbiosciences.com)
- Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA and CD45RB. (cytekbio.com)
- CD45, also referred to as CD45R and PTPRC (Protein tyrosine phosphatase receptor type C), has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. (nsjbio.com)
- CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. (abeomics.com)
- Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. (abeomics.com)
Lymphocytes5
- CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. (thermofisher.com)
- Ex vivo analysis of phenotype and TCR usage in relation to CD45 isoform expression on cytomegalovirus-specific CD8+ T lymphocytes. (ox.ac.uk)
- In some individuals, expansions of antigen-specific CD8+ T lymphocytes bearing specific TCR Vbeta chains were restricted to cells of particular CD45 isoforms. (ox.ac.uk)
- Immunity against CMV comprises a large population of CD8+ T lymphocytes with heterogeneous potential, a spectrum in which CD45 isoform expression may play a central role. (ox.ac.uk)
- CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. (biolegend.com)
Antibodies1
- SARS-CoV-2 antigen-specific humoral and cellular immune responses, including neutralizing antibodies to ancestral virus and variants of concern (VOC), were measured up to six months post vaccination and compared to immune responses in historical cohorts of naïve healthy volunteers (HV) vaccinated with COH04S1 and naïve healthcare workers (HCW) vaccinated with the FDA-approved mRNA vaccine Comirnaty® (Pfizer, New York, NY, USA). (bvsalud.org)
Differ in their extracellular domains2
- CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. (thermofisher.com)
- these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. (exbio.cz)
Tyrosine phosphatase2
- It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. (biolegend.com)
- CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. (adipogen.com)
Common antigens1
- Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. (bdbiosciences.com)
Lymphocyte2
- gp100(209-2M) peptide immunization of human lymphocyte antigen-A2+ stage I-III melanoma patients induces significant increase in antigen-specific effector and long-term memory CD8+ T cells. (bdbiosciences.com)
- CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. (thermofisher.com)
Receptor8
- CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. (thermofisher.com)
- The T cell receptor (TcR) consists of both constant and variable regions, the latter of which determines what antigen the T cell can respond to. (wikidoc.org)
- With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. (cytekbio.com)
- Along with other members of the PTP family, it regulates a number of cellular processes including cell differentiation, growth and mitotic cycle, and is an essential regulator of B- and T-cell antigen receptor-mediated activation. (nsjbio.com)
- Taking advantage of the diverse γδ TCR repertoire or other ligand-receptor interactions, γδ T cells can recognize a broad spectrum of tumor-associated antigens (TAAs) in a major histocompatibility complex (MHC)-independent manner, thereby activating downstream pleiotropic effects. (explorationpub.com)
- CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. (biolegend.com)
- Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. (neobiotechnologies.com)
- Hematopoietic cell transplantation (HCT) and chimeric antigen receptor (CAR)-T cell patients are immunocompromised, remain at high risk following SARS-CoV-2 infection, and are less likely than immunocompetent individuals to respond to vaccination. (bvsalud.org)
T2001
- CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. (biolegend.com)
Exon2
- CD45 contains a Tn antigen in exon B. CD45 has 3 important exons (4,5,6), that are designated A,B,C. Isoforms of CD45 are labeled depending on the presence of these exons. (wikipedia.org)
- CD45RA contains the A exon and is a naive T-cell marker which may help prevent autoimmune disease. (nsjbio.com)
HI301
- Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD45 (HI30) PerCP-Cy™5.5. (exbio.cz)
Protein1
- This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. (anticorps-enligne.fr)
Bind3
- Some APCs also bind native (or unprocessed) antigens to their surface, such as follicular dendritic cells , but unprocessed antigens do not interact with T cells and are not involved in their activation. (wikidoc.org)
- The antigens that bind to MHC proteins are always short peptides , 8-10 amino acids long for MHC Class I, and up to 25 or so for MHC Class II. (wikidoc.org)
- CD45 has also been reported to bind galectin-1 and CD22. (biolegend.com)
Extracellular1
- The variation in these isoforms is localized to the extracellular domain, with the intracellular domain being conserved. (nsjbio.com)
Lymphoid1
- CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. (thermofisher.com)
Exons2
- Multiple isoforms of CD45 are distributed throughout the immune system and arise due to alternative splicing of exons located in the N-terminus. (nsjbio.com)
- These isoforms arise because of alternative splicing of exons 4, 5, and 6. (abeomics.com)
Tonsil1
- Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Ab (2B11). (abeomics.com)
Activation1
- Activation and differentiation antigens on T cells of healthy, at-risk, and HIV-infected children. (bdbiosciences.com)
Hematopoietic3
- CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. (thermofisher.com)
- CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). (cytekbio.com)
- Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. (adipogen.com)
Differentiation2
- Leucocyte typing III : white cell differentiation antigens. (bdbiosciences.com)
- CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. (biolegend.com)
Cells11
- Expansion of CMV-specific CD8+CD45RA+CD27- T cells in B-cell chronic lymphocytic leukemia. (bdbiosciences.com)
- Decreased effector memory CD45RA+ CD62L- CD8+ T cells and increased central memory CD45RA- CD62L+ CD8+ T cells in peripheral blood of rheumatoid arthritis patients. (bdbiosciences.com)
- Antigen presentation stimulates T cells to become either "cytotoxic" CD8+ cells or "helper" CD4+ cells. (wikidoc.org)
- Function of T helper cells: Antigen presenting cells ( APCs ) present antigen on their Class II MHC molecules ( MHC2 ). (wikidoc.org)
- Following T cell development , matured, naïve (meaning they have never been exposed to the antigen to which they can respond) T cells leave the thymus and begin to spread throughout the body, including the lymph nodes . (wikidoc.org)
- Class II MHC proteins are generally only found on the surface of professional antigen-presenting cells (APCs). (wikidoc.org)
- Professional antigen presenting cells are primarily dendritic cells , macrophages and B cells , although dendritic cells are the only cell group that expresses MHC Class II constitutively (at all times). (wikidoc.org)
- During an immune response, professional antigen-presenting cells (APCs) endocytose (absorb) foreign material (typically bacteria or viruses ), which undergoes processing , then travel from the infection site to the lymph nodes . (wikidoc.org)
- Once at the lymph nodes, the APC begins to present antigen peptides that are bound to Class II MHC, allowing CD4 + T cells that express specific TcR's against the peptide/MHC complex to activate. (wikidoc.org)
- Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. (exbio.cz)
- Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. (exbio.cz)
Crucial1
- Combinations of CD45 isoforms are crucial for immune function and disease. (exbio.cz)
Apoptosis3
- CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions. (thermofisher.com)
- Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. (exbio.cz)
- Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. (exbio.cz)
Expression5
- CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. (thermofisher.com)
- Although the detailed composition of these pools is not completely understood, there is known to be heterogeneity, in particular of CD45 isoform expression. (ox.ac.uk)
- In particular, we examined expression of these phenotypic markers in relation to the expression of CD45 isoforms. (ox.ac.uk)
- We found that a spectrum of phenotypes exists stably, from CD45R0(high)/RA(low) through CD45RA(high)/R0(low), and that expression of other surface markers such as CD28 and CD62L, and also TCR usage, may vary in parallel with CD45 isoform expression. (ox.ac.uk)
- CD45 isoform expression can change in response to cytokines. (biolegend.com)
Functions1
- Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections 9 , inhibition of CD45 functions 4 , immunofluorescence 11 , Western blotting 3 , and spatial biology (IBEX) 16,17 . (biolegend.com)
Includes1
- This includes the Tn antigen (GalNAc O-bound to serine or threonine) which is prominently expressed on carcinomas, where it can also be sialylated. (wikipedia.org)
Surface1
- CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. (biolegend.com)
Complex1
- Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. (antibodies-online.com)