Cd44 and cd24. Medical search. Frequent questions

After analyzing different tumor stem cell markers (SOX2, OCT4, CD133, CD44 and CD24) in pancreatic cancer cells treated with different chemotherapeutic protocols by means of RT-qPCR, Oxaliplatin and Gemcitabine in monotherapy were the chemotherapies that selected the most cancer stem cells while the FOLFIRI protocol decreased them. (isciii.es)
Tras analizar mediante RT-qPCR diferentes marcadores de células madre tumorales (SOX2, OCT4, CD133, CD44 y CD24) en células de cáncer de páncreas tratadas con diferentes protocolos quimioterapéuticos, el Oxaliplatino y la Gemcitabina en monoterapia fueron los quimioterápicos que seleccionaron en mayor medida las células madre cancerosas mientras que el protocolo FOLFIRI las disminuyó. (isciii.es)
CAE induced a dose-dependent increase in cell migration and invasion and also in the expression of cancer stem cells markers (CD133, CD44, CD90). (springer.com)
A quantity of cell surface markers such as CD44, CD24, and CD133 are often used to identify and enrich CSCs. (medgadget.com)
For lung cancers, CSC markers include CD44 and CD133 (PROM1), but also CD117 (KIT), CD90 (or THY1), CD166, EpCAM for non-small-cell lung carcinoma (NSCLC), and PODXL-1, PTCH, and CD87 for small-cell lung carcinoma (SCLC) 25 . (atlasantibodies.com)
8. Wright MH, Calcagno AM, Salcido CD, Carlson MD, Ambudkar SV, Varticovski L. Brca1 breast tumors contain distinct CD44 + /CD24 - and CD133 + cells with cancer stem cell characteristics. (southernbiotech.com)
Expression profiles of non-CD133 CRCSC (CD29, CD44, ALDH1A1, ALDH1B1, EpCam, and CD166) were detected by immunohistochemistry and the association with clinicopathological data and patient outcomes was determined using standard statistical methodology. (jcancer.org)
These findings link iNOS to Notch1 signaling in CD24(+)CD133(+) LCSCs through the activation of TACE/ADAM17. (cusabio.com)

Aims: To determine the validation of the CSC model for cell surface markers such as CD44 and CD24 and their clinical significance. (nbuv.gov.ua)
Polyak previously found that triple-negative tumors typically contain a large number of "stem-like" breast cancer cells, labeled CD44+CD24- cells, referring to identifying markers on their surfaces. (harvard.edu)
On the other hand, in brain tumors, such as glioma and glioblastoma, CSCs have instead been identified using cell surface markers, including SSEA-1 20 , EGFR 21 , and CD44 22 . (atlasantibodies.com)
Tanshinone IIA attenuated the stemness of breast cancer cells, evident by downregulating the expression of stemness markers, hindering the capacity of spheroid formation, decreasing the CD24 − /CD44 + sub-population in a concentration-dependent manner and reducing the tumor-initiating ability of breast cancer cells. (biomedcentral.com)

Signal transducer CD24 also known as cluster of differentiation 24 or heat stable antigen CD24 (HSA) is a protein that in humans is encoded by the CD24 gene. (wikipedia.org)

An analysis of 12 human breast cancer tumors found an even higher correlation of 95.5 percent between GD2+ cells and CD44 high/CD24 low status. (weeksmd.com)
As a result, Polyak and colleagues believe that new treatments aimed at specifically knocking out these CD44+CD24- cells with activated Jak2/Stat3 signaling could be useful in combating triple-negative cancers and potentially other tumors that contain these cells. (harvard.edu)
The investigators surveyed genes present in CD44+CD24- cells and found 1,576 genes that differed from those in other, more differentiated epithelial cancer cells within tumors. (harvard.edu)
Studies have shown that CD44, also known as HCAM, plays an important role in the cascade of metastasis and progression of human malignant tumors (2). (biocare.net)
Suppression of human breast tumors in NOD/SCID mice by CD44 shRNA gene therapy combined with doxorubicin treatment. (vinastemcelllab.com)

Open in a separate window Number 4 The addition of hyperthermia to radiation significantly reduced cell proliferation and viability in breast CSCs and pancreatic CSCs(A) The surviving fraction of CD44+CD24C CSCs based on cell number counting at 24, 48, 72 hours after indicated treatments. (monossabios.com)
B) The surviving fraction of CD44+CD24+ESA+ CSCs based on cell number counting at 72 hours after indicated treatments. (monossabios.com)
C) The surviving fraction of CD44+CD24C CSCs based Abiraterone kinase activity assay on cell viability was recognized by CCK-8 at 48 hours post indicated treatments. (monossabios.com)

Conclusions: As a result of our research, the analysis of the quantity of assumed stem cells of the BC, which were identified by immunohistochemistry as CD44 and CD24 cells, failed to detect a statistically significant relation between groups of patients with different prognosis, and the identification of their expression is not enough for the characteristics of CSC. (nbuv.gov.ua)

FITC Anti-Mouse CD44 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blot assays. (southernbiotech.com)
The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. (southernbiotech.com)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA254759(CD24 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (cusabio.com)
G7mAb was an anti-CD24 antibody. (cusabio.com)

Figures show, from left to right: immunofluorescence (human mammary adenocarcinoma cell), WB (Lane 1: no treated, Lane 2: non-targeting siRNA treated sample, Lane 3: CD44 siRNA treated sample #1, Lane 4: CD44 siRNA treated sample #2), IHC (breast invasive ductal carcinoma) and Flow Cytometry (human cancer cell line). (tebubio.com)
In vitro mammary spheroid formation, flow cytometry assay on CD24 − /CD44 + sub-population, ALDH activity detection, cell viability assay and western blot analysis, and in vivo tumor-initiating analysis were performed to examine the effects of Tanshinone IIA on the stemness of breast cancer cells. (biomedcentral.com)

For instance, Zhang et al ( 23 , 24 ) found that RUNX2 was required for the activity of CD44 + /CD24 −/low BCSCs during breast cancer development, while miR-205/RUNX2 axis was further identified with s negative regulatory effect upon the activity of CD44 + /CD24 − BCSCs. (spandidos-publications.com)
The miRNA profile between breast cancer stem cells(BCSCs, CD44+CD24-/low) and control MCF-7 breast cancer cells was obtained in a previous study. (researchsquare.com)
Previous studies have confirmed that miRNAs play an important role in gene regulation in CD44 + CD24 -/low breast cancer stem cells (BCSCs). (researchsquare.com)
In our previous research, we performed miRNA profiling between sorted CD44 + CD24 -/low BCSCs and the control MCF-7 breast cancer cells [8]. (researchsquare.com)

When the activity of several of those genes was blocked in CD44+/CD24- tumor cells, the level of Stat3 signaling was reduced and cell growth was suppressed, the researchers said. (harvard.edu)

A group of cells with high expression of CD44 high /CD24 low or ALDH + in breast cancer are considered to be breast cancer stem cells (BCSC) [ 6 ] . (ijbs.com)

CD44 and CD24 antigens expression was evaluated by the percentage of positive cells using different chromogens and the MultiVision detection system by immunohistochemical method. (nbuv.gov.ua)
We analysed the expression of CD44, CD24 and CD44⁺/CD24⁻ for different patients with dividing them into two groups. (nbuv.gov.ua)
The distribution of the expression of CD24 marker did not differ between the groups (p = 0.36) as well as the OS curves (p = 0.59). (nbuv.gov.ua)
Nevertheless, the comparison of the clinical outcomes did not reveal a statistically significant difference in the survival curves in the groups with existence and absence of CD44⁺/CD24⁻ expression (p = 0.08). (nbuv.gov.ua)
The obtained data demonstrating the worst clinical outcome in the cases of absence of CD44⁺/CD24⁻ expression apparently require further investigations and the validation of the immunohistochemical method with the determination of the cut-off line in defining of CD44 and CD24 status. (nbuv.gov.ua)
A known combination marker of cancer stem cells is high expression of CD44 and low expression of CD24 surface proteins. (weeksmd.com)
Comparing gene expression between GD2+ cells and CD44 high/CD24 low cells revealed 100 percent correlation in the expression of 231 genes. (weeksmd.com)
In breast cancer studies, evaluation of CD44 expression has been useful in the differentiation of benign and malignant papillary lesions (2). (biocare.net)
Expression of CD44s, the most common CD44 isoform, has been reported to be associated with increased survival of patients with nodenegative invasive breast carcinoma (3). (biocare.net)
Our results suggest that higher CD24 expression is significantly associated with lower OS rate, lower DFS rate and some clinicopathological factors such as lymph node invasion and TNM stage. (cusabio.com)

CD24 and CD44 are upregulated in human pancreatic cancer compared to chronic pancreatitis and may be related to the development of pancreatic cancer. (cusabio.com)

CD24 gene is found on chromosome 6 (6q21) An alignment of this gene's sequence finds genomic locations with similarity on chromosomes 1p36, 3p26, 15q21.3, 20q11.2 and Yq11.222. (wikipedia.org)

Are CD44⁺/CD24⁻ cells the assumed cancer stem cells in breast cancer? (nbuv.gov.ua)
The content of CD44 cells did not differ statistically between groups A and B (p = 0.18), but there was a tendency for increasing in OS with the existence of CD44+ cells (p = 0.056). (nbuv.gov.ua)
The researchers found 85 percent of GD2-positive breast cancer cells were CD44 high/CD24 low, while only 1 percent of GD2-negative cells shared that characteristic. (weeksmd.com)
Additional experiments assessing the viability of CD44+CD24- cells when each of these genes was inhibited individually narrowed the field to 15 genes that were required for their growth and thus looked like promising targets for selective drugs. (harvard.edu)
BALB/c mouse bone marrow cells were stained with Rat Anti-Mouse CD44-FITC (SB Cat. (southernbiotech.com)
CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. (southernbiotech.com)
Further studies have shown a subpopulation of CD44+/CD24- in breast cancer cells have stem/progenitor cell properties (4). (biocare.net)
Downregulation of CD44 reduces doxorubicin resistance of CD44+CD24- breast cancer cells. (vinastemcelllab.com)
Differentiation of breast cancer stem cells by knockdown of CD44: promising differentiation therapy. (vinastemcelllab.com)
Roles of CD44 in stem cells and cancer stem cells. (vinastemcelllab.com)
CD24, a cell surface receptor enriched in both juvenile chondrocytes and human induced pluripotent stem cells-derived chondrocytes, is a regulatory factor in both faster proliferation and resistance to proinflammatory cues in these chondrocyte populations. (cusabio.com)
For CD44+CD24+ESA+ cells, is definitely 0.148, is 0.045, mean surviving fraction at 2 Gy [SF2Gy] = 0.62. (monossabios.com)
The results showed that CD44+CD24+ESA+ cells were more radioresistant than L3.6pl cells. (monossabios.com)
Taken together, sorted CD133+ cells or CD44+CD24+ESA+ cells showed the enhanced tumorigenic potential, improved manifestation of the stemness related molecule Sox2 and highly resistant to radiation, showing their stem cell properties as earlier works reported. (monossabios.com)

Monoclonal antibodies to Pgp-1-CD44 block lympho-hemopoiesis in long-term bone marrow cultures. (southernbiotech.com)

Involvement of CD44 in mast cell proliferation during terminal differentiation. (southernbiotech.com)

CD24 is a cell adhesion molecule. (wikipedia.org)
CD44 primarily functions as an adhesion molecule. (southernbiotech.com)
We believe that CD24 is a key molecule of metastatic progression in the epithelial-mesenchymal-transition phenomenon and a promising therapeutic target for advanced ovarian cancer. (cusabio.com)

Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. (cusabio.com)

CD44 regulates hematopoietic progenitor distribution, granuloma formation, and tumorigenicity. (southernbiotech.com)

Notable downregulation of the enriched stemness of secondary mammosphere was detected by the significantly decreased percentage of CD44 + /CD24 -/low population after eugenol treatment along with their distorted morphology and smaller the number of spheres. (nih.gov)

Ethnicity and genetic predisposition are partly responsible for this heterogeneity, evidenced by the finding that CD44+/CD24- and PROCR+/EpCAM- multi-potent stem cells were elevated significantly in African American women compared with Caucasians. (nature.com)

The CD44(+)/CD24(-/low) subpopulation of MCF10DCIS cells showed elevated Notch1 signaling and increased cell proliferation compared to the CD44(+)/CD24(high) subpopulation. (nih.gov)

3. Create a child gate from P3 that includes CD44- and CD271- cells (P4) 4. (bdbiosciences.com)

Flow Cytometry: CD24 Antibody (ML5) [NB100-77903] - Analysis using the FITC conjugate of NB100-77903. (novusbio.com)
The expression of CD44 + CD24 - was measured by flow cytometry. (biomedcentral.com)
To clarify the mechanisms of CD44+CD24- cells resistance to hypoxia-induced apoptosis is achieved by inhibiting the activation of endoplasmic reticulum stress (ERS) pathway, silenced CHOP and PERK cell lines of EC9706 cells and overexpressed CHOP and PERK cell lines of CD44+CD24- cells were constructed, the effects of hypoxia on apoptosis, cell cycle, and mitochondrial membrane potential were detected by flow cytometry and JC-1 reagent. (bvsalud.org)

Some studies showed that estrogen treatment increases BCSC properties, including mammosphere forming efficiency and the proportion of CD44+/CD24− cells, in ER+ breast cancer cell lines and that this effect was reversed by endocrine therapy, suggesting that ER is necessary for stem cell expansion [ 13 , 14 ]. (biomedcentral.com)
In addition to these studies, it has also been suggested that the ERα-36 variant can promote BCSC self-renewal and increase the CD44+/CD24− cell population [ 17 , 18 ]. (biomedcentral.com)

Immunocytochemistry/ Immunofluorescence: CD24 Antibody (ML5) [NB100-77903] - MCF-7 breast cancer cells. (novusbio.com)
Based on the literature and availability of antibody, highly expressed genes (CD24, CD44, and CD74) encoding cell surface receptors and (FABP5 and heat shock protein Hsp27) encoding cytoplasmic proteins were evaluated at protein levels by IHC. (biomedcentral.com)

Cross-linking of CD24 on the surface of neutrophils induces apoptosis, and this appears to be defective in sepsis. (wikipedia.org)
The three apoptosis pathways were not affected by hypoxia in CD44+CD24- cells (P>0.05). (bvsalud.org)

We previously reported that a Gemini vitamin D analog, 1,25-dihydroxy-20R-21(3-hydroxy-3-deuteromethyl-4,4,4-trideuterobutyl)-23-yne-26,27-hexafluoro-cholecalciferol (BXL0124), reduced CD44(+)/CD24(-/low) cells in MCF10DCIS basal-like breast cancer cells. (nih.gov)

To identify CD44 + /CD24 - cells in TM40D cell line, we measured expression of CD44 + CD24 - at passages 2-16 by using a fluorescence-activated cell sorting (FACS)-Vantage SE instrument (Becton Dickinson, Franklin Lakes, NJ). (biomedcentral.com)
Methods: CD44+CD24- cells in EC9706 cells were screened by fluorescence-activated cell sorting (FACS). (bvsalud.org)

CD44, CD24 and ESA were also found to play a role in breast cancer stem cells. (sciencedaily.com)
A study published in January 2007 by U-M and Stanford University researchers narrowed the field for head and neck cancer stem cells, again finding that cells expressing CD44 were involved. (sciencedaily.com)
CD24 encodes a cell surface glycoprotein that has emerged as a marker for mammary stem cells. (micrornainhibitor.com)

CD24 and CD52 had been observed to get expressed at larger levels in mammary glands from E2 treated ACI rats, relative to BN rats. (micrornainhibitor.com)
In the mouse mammary gland, Cd24 is expressed during the luminal epithelium and also to a lesser extent during the basal epithelium. (micrornainhibitor.com)
Mice which can be homozygous for any Cd24 null allele exhibit accelerated ductal elongation and enhanced branching morphogenesis in the mammary gland. (micrornainhibitor.com)

Interestingly, CD44 hi CD24 lo cells purified from normal and malignant breast cancer tissue exhibit features of an EMT, and human cancer cells induced to undergo EMT exhibit stem cell-like properties and increased metastatic potential (84). (jci.org)
Interestingly, the interaction among CD44 and Mmp9 in PC3 prostate cancer cells has been demonstrated Inhibitors,Modulators,Libraries to be induced by Spp1. (micrornainhibitor.com)

The antibodies used were anti-CD24-phycoerythrin (PE), anti-CD44-PE-cyainn 5 (CY5), and their corresponding isotype controls (BioLegend, San Diego, CA). Cells were harvested and gently disaggregated to a single cell suspension. (biomedcentral.com)

It also enhanced the survival significantly in Balb/C mice injected with isolated CD44 + /CD24 − CSC population apart from increasing the bone mineral density and ameliorating lytic bone lesions. (medscape.com)
Finally, our results support the view that GDF3 has the ability to induce progression of CD24-inducible melanoma in mice. (biomedcentral.com)

Two polymorphisms of CD44 3'UTR weaken the binding of miRNAs and associate with naso-pharyngeal carcinoma in a Chinese population. (cdc.gov)
Association of CD44 polymorphisms and susceptibility to HBV-related hepatocellular carcinoma in the Chinese population. (cdc.gov)

CD24, CD44, and CD74 proteins were expressed on the cell surface, and FABP5 and HSP27 proteins were predominantly expressed in the cytoplasm of HNSCC. (biomedcentral.com)

Results: Of the 223 cancers in the study, 182 were positive for CD44, 83 for CD24 and 52 for ALDH1A1. (elsevierpure.com)

Ectopic expression of GDF3 was accompanied by an increased level of production of CD24/CD44. (biomedcentral.com)

CD24 crosslinking induces a Ca2+ flux in mature B cells. (novusbio.com)

Here it is shown that the SP and the CD44 + /CD24 -/low populations are overlapping. (elsevierpure.com)

The nanoparticles showed good inhibitory effects on CD44 + /CD24 − CSC population isolated from SP2/0 murine myeloma cells. (medscape.com)
Mammosphere assays have been performed with SUM149, as well as with MDA MB 231 cells, which hugely expresses CD44 in about 90% of its population, in ultra minimal attach ment 6 nicely culture plates in finish Mammocult media, as previously described. (plasignaling.com)

Since GDF3-driven CD24 acts as a receptor for endogenous innate immune ligands that modulate cell proliferation, CD24 is an effective determinant of tumorigenesis in malignant cell transformation. (biomedcentral.com)

CD24 may play a role in the regulation of B-cell proliferation and maturation. (novusbio.com)

CD52, which is paralagous to CD24, is expressed by lymphocytes and other styles of immune cells. (micrornainhibitor.com)

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