Cd38 progenitor cells or bone marrow. Medical search. Frequent questions

We examined the CD45 dim CD34 + CD38 - CD133 + cells on bone marrow samples of hematologic malignancies and healthy controls using four-color flow cytometry experiments. (researchsquare.com)
Interestingly, the CD45 dim CD34 + CD38 - CD133 + cells were highly expressed in bone marrow of patients with AML compared to that of healthy controls (HC). (researchsquare.com)
Additionally, the high levels of the CD45 dim CD34 + CD38 - CD133 + cells in AML patients were an independently significant poor risk factor for overall survival and event free survivals. (researchsquare.com)
Therefore, our results suggest that CD45 dim CD34 + CD38 - CD133 + cells in AML might have the potential of leukemia stem cells. (researchsquare.com)
For examples, Rhenen et al showed that a high percentage of CD34 + CD38 - stem cells at diagnosis significantly correlated with a high minimal residual disease frequency and subsequently to relapse especially after the third course of chemotherapy in AML patients. (researchsquare.com)
Moreover, the CD34 + CD38 - progenitor cells expressed variable amounts of the target receptor CD33, CD133 and c-kit (CD117) [ 20 ]. (researchsquare.com)
Furthermore, it was developed using the basic platform of the CD34 + CD38 - markers, but applying more advanced antigens such as CD45 dim and CD133. (researchsquare.com)
Mouse anti-human CD45-FITC (Clone 2D1, Cat No. 347463), Mouse Anti-human CD34-PE [Clone 8G12 (also known as HPCA2), Cat No. 348057], Mouse anti-human CD38-PE-Cy™5 (Clone HIT2, Cat No. 555461) and appropriated isotype control antibodies were purchased from BD Biosciences (San Diego, CA, USA). (researchsquare.com)
CD34 derives its name from the cluster of differentiation protocol that identifies cell surface antigens. (wikipedia.org)
CD34 was first described on hematopoietic stem cells independently by Civin et al. (wikipedia.org)
The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early haematopoietic and vascular-associated progenitor cells. (wikipedia.org)
CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. (wikipedia.org)
Conversely, under other circumstances CD34 has been shown to act as molecular "Teflon" and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumina. (wikipedia.org)
Finally, recent data suggest CD34 may also play a more selective role in chemokine-dependent migration of eosinophils and dendritic cell precursors. (wikipedia.org)
Regardless of its mode of action, under all circumstances CD34, and its relatives podocalyxin and endoglycan, facilitates cell migration. (wikipedia.org)
The presence of CD34 on non-hematopoietic cells in various tissues has been linked to progenitor and adult stem cell phenotypes. (wikipedia.org)
Later studies have reported that low rhodamine retention identifies LT-HSCs within the Lin−CD34+CD38− population. (wikipedia.org)
CD34 is expressed in roughly 20% of murine haematopoietic stem cells, and can be stimulated and reversed. (wikipedia.org)
In the present study, we demonstrate that functional CD34 + progenitor cells can be efficiently produced from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) by combined modulation of 2 signaling pathways. (ashpublications.org)
A higher proportion of CD34 + cells (∼ 20%) could be derived from hPSCs by inhibition of mitogen-activated protein kinase (MAPK) extracellular signal-regulated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling and activation of bone morphogenic protein-4 (BMP4) signaling. (ashpublications.org)
hPSC-derived CD34 + progenitor cells further developed to endothelial and smooth muscle cells with functionality. (ashpublications.org)
Our results suggest that combined modulation of signaling pathways may be an efficient means of differentiating hPSCs into functional CD34 + progenitor cells. (ashpublications.org)
To identify regulators of primitive chronic myeloid leukemia (CML) cells, we performed a high-content cytokine screen using primary CD34 + CD38 low chronic phase CML cells. (haematologica.org)
Distinct requirements for optimal growth and In vitro expansion of human CD34(+)CD38(-) bone marrow long-term culture-initiating cells (LTC-IC), extended LTC-IC, and murine in vivo long-term reconstituting stem cells. (ox.ac.uk)
Such extended long-term culture-initiating cells (ELTC-IC) are of the CD34(+)CD38(-) phenotype, are quiescent, and are difficult to recruit into proliferation, implicating ELTC-IC as the most primitive human progenitor cells detectable in vitro. (ox.ac.uk)
Here, CD34(+)CD38(-) BM ELTC-IC (12-week) were efficiently recruited into proliferation and expanded in vitro in response to early acting cytokines, but conditions for expansion of ELTC-IC activity were distinct from those of traditional (5-week) LTC-IC and murine long-term repopulating cells. (ox.ac.uk)
Expansion of LTC-IC was most optimal after 7 days of culture, whereas optimal expansion of ELTC-IC activity required 12 days, most likely reflecting the delayed recruitment of quiescent CD34(+)CD38(-) progenitors. (ox.ac.uk)
We observed that Lin-CD34+CD38+ progenitor cells in 8 out of the 10 MDS patients had higher SALL4 expression levels, while the progenitor cells of normal BM cells did not express SALL4. (confex.com)
Similar results were observed in Lin-CD34+CD38- hematopoietic stem cells, CD11b+ mature myeloid cells, CD19+ B cells, CD235+ erythroid cells. (confex.com)
Using the non-obese diabetic (NOD)/severe combined immunodeficiency disease (SCID) mouse model, a CD34 + CD38 - cell fraction representing 0.1-1% of the leukaemic cell population was shown to contain the leukaemia-initiating cell or leukaemic stem cell (LSC). (touchoncology.com)
Leukaemia stem cells (LSC) are identified by high CD34 and negative CD38 expression. (ghrnet.org)
The CD34 protein is definitely regularly used as a marker for positive selection of human being hematopoietic come and progenitor cells. (woofahs.com)
However, Goodell et al[24, 25] characterized murine Hoechst dye-excluding part human population cells that lacked CD34 appearance and experienced reconstitution capacity. (woofahs.com)
26] shown a low level of engraftment activity in human being CD34- cells. (woofahs.com)
We showed that highly purified human being CD34+ cells generated CD34? (woofahs.com)
cells in immune-deficient mice after 1?year and that the reisolated human CD34? (woofahs.com)
We determined that CD34 expression at the cell surface and messenger RNA (mRNA) level can be turned on and off, relating to the quiescence of the human hematopoietic stem cell population in vivo [27]. (woofahs.com)
When reisolated from the marrow of beige/nude/xid mice, the human cells were lacking of CD34 expression absolutely. (woofahs.com)
Although CD38-CAR T cells lysed CD38 + monocytes, NK cells, CD34 + cells and to a lesser extent CD38 + T and B cells, they did not hamper the outgrowth progenitor cells into various myeloid lineages. (biomedcentral.com)
These progenitors termed multi-lymphoid progenitors (MLP 13 or lymphoid-primed multipotent progenitors (LMPP 15 by analogy with the mouse system 16 are identified as CD34+CD38?Thy1?CD45RA+ 13 15 or by high expression of L-selectin on CD34+ cells 17. (biopaqc.com)
About 35% to 40% of CD34(+) and CD34(+)CD38(-) stem and progenitor cells adhered to laminin-10/11, and 45% to 50% adhered to fibronectin, whereas they adhered less to laminin-8 and laminin-1. (ox.ac.uk)
Adhesion of CD34(+)CD38(-) cells to laminin-10/11 was maximal without integrin activation, whereas adhesion to other proteins was dependent on protein kinase C activation by 12-tetradecanoyl phorbol-13-acetate (TPA). (ox.ac.uk)
Fluorescence-activated cell-sorting (FACS) analysis showed expression of integrin alpha6 chain on most CD34(+) and CD34(+)CD38(-) cells. (ox.ac.uk)
In functional assays, both laminin-8 and laminin-10/11 facilitated stromal-derived factor-1alpha (SDF-1alpha)-stimulated transmigration of CD34(+) cells, by an integrin alpha6 receptor-mediated mechanism. (ox.ac.uk)
Moreover, in human studies, JAK2V617F has been identified in the stem cell-enriched CD34 + CD38 − CD90 + Lin − compartment, common myeloid progenitors, granulocyte-monocyte precursors, and megakaryocyte-erythroid precursors as well as natural killer cells, B cells, and T cells in some patients, confirming its origin in an early hematopoietic progenitor. (oncohemakey.com)
CSCs were first identified in hematological cancer such as acute myeloid leukemia in the late '90s when scientists isolated a subpopulation of leukemia cells that expressed the surface marker CD34 but not CD38. (atlasantibodies.com)
The patients were evaluated based on PBSC mobilization and collection parameters, including overall collection results, CD34 + cell levels in peripheral blood, leukapheresis (LP) delays, overall number of LP sessions, and the rate of rescue mobilization with plerixafor. (biomedcentral.com)
Graded numbers of CD34+CD38-Lin- HSC from bone marrow and cord blood had been co-cultured with OP9-GFP stromal cells and had been phenotypically assayed just after 2-3 weeks for the presence in the following populations: undifferentiated CD34+ HSC, CD14+HLA-DR+ monocytes and CD15+ granulocytes. (idhinhibitor.com)
Graded numbers of CD34+CD38-Lin- HSC from bone marrow and cord blood were co-cultured with OP9-DL1 stromal cells, and assayed phenotypically IRAK4 medchemexpress immediately after 4-5 weeks for the presence ofFaster and more substantial T-cell differentiation by cord blood hematopoietic stem cellsGiven this reduction in T-lineage prospective in adult bone marrow HSC. (idhinhibitor.com)
Whereas normal CD34 + /CD38 − bone marrow stem cells displayed only low amounts of CD25 or lack CD25, CD34 + /CD38 − leukemic stem cells strongly expressed CD25 in over 90% of all patients with untreated chronic myeloid leukemia. (stemcellsciencenews.com)

CD34+ is often used clinically to quantify the number of haemopoietic stem cells for use in haemopoietic stem cell transplantation. (wikipedia.org)
Antibodies are used to quantify and purify hematopoietic progenitor stem cells for research and for clinical bone marrow transplantation. (wikipedia.org)
Recent medical advances in the field of regenerative medicine and tissue transplantation have highlighted the importance of umbilical cord blood (UCB) as a valuable alternative source of haematopoietic stem cells, which are potentially life-saving in a vast array of clinical applications. (sajbl.org.za)
Hematopoietic stem cells are widely used in medical treatments, particularly in bone marrow transplantation for individuals with hematological disorders like leukemia or aplastic anemia. (thenoveldifference.com)
Induction therapy followed by CD34+ cell mobilisation and autologous transplantation represents standard of care for multiple myeloma (MM). However, the anti-CD38 monoclonal antibodies daratumumab and isatuximab have been associated with mobilisation impairment, yet the mechanism remains unclear. (bvsalud.org)
In other fractions, only clonogenic leukaemia progenitors were found, and these were unable to regenerate the tumour after serial transplantation in mice. (touchoncology.com)
cells retained the capacity to regenerate CD34+ cells upon secondary transplantation. (woofahs.com)
These early studies demonstrated that expression of JAK2V617F in retroviral bone marrow transplantation models caused erythrocytosis, confirming its relevance to human MPNs. (oncohemakey.com)
We also demonstrate that C3AR in combination with GPR56 distinguishes the leukemic stem cells (LSCs) in NPM1-mutated AML from the normal hematopoietic stem cells, defining the LSC population, as shown by transplantation into immunodeficient mice. (lu.se)
Therapies for these disorders (eg, intravenous immunoglobulin [IVIG], bone marrow transplantation, gene therapy) are very costly and require highly advanced facilities. (medscape.com)
Autologous hematopoietic stem cell transplantation and reconstitution: Different species and including primates and humans for studies are being used for development, localization, and therapeutic strategies. (hoajonline.com)
Pre-emptive treatment with rituximab of molecular relapse after autologous stem cell transplantation in mantle cell lymphoma. (smw.ch)
Multiple research groups have developed artificial mitochondrial transfer/transplantation (AMT/T) methods that transfer healthy mitochondria into damaged cells and recover cellular function. (biomedcentral.com)
Allogeneic hematopoietic stem cell transplantation (HSCT) offers a curative treatment for acute myeloid leukemia patients. (oncotarget.com)
Through in vitro cell culture and transplantation tumor model experiments, the researchers found that acute myeloid leukemia cells specifically destroyed the microenvironment of fat cells in the bone marrow, resulting in an unbalanced regulation of hematopoietic stem cells and progenitor cells and curbing the production of normal blood cells in the bone marrow. (cancerfax.com)
The choice of chemotherapy depends on several factors, including the patient's performance status, age, renal function, desire for inpatient or outpatient therapy, and likelihood of receiving future autologous stem cell transplantation. (medscape.com)

Differentiation of human pluripotent stem cells (hPSCs) into functional cell types is a crucial step in cell therapy. (ashpublications.org)
UCB's advantages over other sources of stem cells such as adult bone marrow and embryonic stem cells include easy procurement, minimal risk to the donor, excellent proliferation and differentiation, immediate availability, and autologous use. (sajbl.org.za)
Thrombopoietin (Tpo), the ligand for c-mpl and a principal regulator of megakaryocytopoiesis and platelet production, has been demonstrated to stimulate the growth and differentiation of megakaryocyte as well as multipotent hemopoietic progenitor cells. (ox.ac.uk)
Progenitor cells, also known as precursor cells, are a type of cell that falls somewhere between stem cells and fully differentiated, specialized cells in terms of their differentiation potential. (thenoveldifference.com)
They have already committed to a particular differentiation pathway, meaning they are destined to become a specific type of cell, such as a red blood cell, white blood cell, or platelet in the case of hematopoietic progenitor cells. (thenoveldifference.com)
Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp. (research.com)
microRNAs have emerged as master regulators of stem cell lineage differentiation and angiogenesis [ 14 ]. (biomedcentral.com)
Understanding how differentiation programs originate from within the gene expression scenery of hematopoietic stem cells (HSC) is vital to develop new clinical therapies. (biopaqc.com)
In the molecular level targeted practical studies of solitary or combined transcription factors possess identified DZNep a relatively DZNep small number of key transcription factors that travel differentiation of progenitor cells by directing the sequential establishment of transcriptional programs essential for terminal differentiation 1. (biopaqc.com)
To understand how stem lymphoid and myeloid programs are coordinated during hematopoietic differentiation we systematically profiled the transcriptome of MLP in the context of nine additional human being hematopoietic stem and progenitor cell populations for which self-renewal and. (biopaqc.com)
Importantly, the frequency was two times greater in cord blood than in bone marrow HSC when the possible to differentiate into CD5+CD7+ early T cells was evaluated, and this enhanced to a 3-fold distinction when CD5+CD7+CD1+CD4+ committed T-lineage precursors had been scored at a later stage of differentiation. (idhinhibitor.com)
In parallel, limiting dilution assays have been performed to compare the myeloid differentiation capacity of bone marrow and cord blood HSC. (idhinhibitor.com)
This reduced differentiation of CD34+ hematopoietic progenitor stem cells could occur due to both infectious and non-communicable diseases and also due to their drug induced treatments. (hoajonline.com)
Retention of cellular phenotype is the hallmark of differentiation of pluripotent stem cells. (hoajonline.com)
Several investigations [ 5 - 7 ] have been carried out with isolated embryonic, fetal, and adult SCs in a well-defined culture microenvironment to define the sequential steps and intracellular pathways that are involved in their differentiation into the specific cell lineages. (biomedcentral.com)
CSCs take charge of a cancer hierarchy, harboring stem cell-like properties involving self-renewal and aberrant differentiation potential. (bmbreports.org)
The authors found that paired related homeobox 1, a homeodomain transcription factor that was previously reported to control skeletal development, is expressed in cortical neural progenitors and is required for their self-renewal and proper differentiation. (stemcellsciencenews.com)
Accordingly, scientists have developed protocols for the expansion, genetic modification and differentiation of stem cells to NK cells. (molcells.org)
Despite growing experience and knowledge concerning human MSC and their use in cell-based strategies, the molecular mechanisms that govern MSC self-renewal, expansion and multilineage differentiation are not well understood and remain an active area of investigation. (biomedcentral.com)
We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
Depending on the tissue of origin, they may have a higher preference for differentiation into one particular cell type and a lower preference for another one, but they all still retain the differentiation potential for the mesenchymal lineage. (promocell.com)
Which plates does PromoCell recommend as 96-well U bottom suspension plates for chondrogenic differentiation of Human Mesenchymal Stem Cells? (promocell.com)
For chrondrogenic differentiation it is important that the cells do not adhere to the wells. (promocell.com)
During differentiation, the cells form spheroids which float in the medium. (promocell.com)
This effect is not only due to the hematopoietic microenvironment of the bone marrow, that is, the niche is crowded, but also the role of adipocytes in the differentiation process. (cancerfax.com)

Acute myeloid leukemia (AML) is generally regarded as a stem cell disease, and it originates from a hierarchy of leukemic stem cell classes that differ in self-renewal capacity [ 1 , 2 ]. (researchsquare.com)
5 Homing and stem cell expansion are being researched to overcome these obstacles militating against wider use of UCB. (sajbl.org.za)
CD34+/CD38+) or more rarely to the stem cell pool (i.e. (altmeyers.org)
Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm caused by an acquired 9;22-chromosomal translocation in a hematopoietic stem cell (HSC) resulting in the expression of the BCR-ABL1 fusion protein. (haematologica.org)
The distinct requirements for expansion of ELTC-IC activity when compared with traditional LTC-IC suggest that the ELTC-IC could prove more reliable as a predictor for true human stem cell activity after in vitro stem cell manipulation. (ox.ac.uk)
The ability of Tpo to promote adhesion to fibronectin was comparable to that of IL-3, but less than that of stem cell factor. (ox.ac.uk)
The key difference between hematopoietic stem cells and progenitor cells is, Think of the hematopoietic stem cell as the ultimate multitasker. (thenoveldifference.com)
Hematopoietic stem cells (HSCs) are a type of multipotent stem cell found primarily in the bone marrow and, to a lesser extent, in peripheral blood. (thenoveldifference.com)
SALL4 is a transcription factor that is important for development and embryonic stem cell properties. (confex.com)
His Cell biology research is multidisciplinary, relying on both Embryonic stem cell, Stromal cell, Pathology and Immune system. (research.com)
Thomas Rülicke spends much of his time researching Cancer research, Leukemia, Stem cell, CD38 and Progenitor cell. (research.com)
His Stem cell study incorporates themes from Multiple forms, Chronic myelomonocytic leukemia and Bone marrow. (research.com)
An emerging idea in MM research is the concept of the cancer stem cell, which has been identified in other cancer types in an attempt to explain incurability. (touchoncology.com)
The cancer stem cell is defined in the same manner as regular tissue stem cells. (touchoncology.com)
On behalf of the Myeloma Stem Cell Network (MSCNET). (touchoncology.com)
One approach for obtaining new knowledge of the pathogenesis of MM is the cancer stem cell (CSC) hypothesis. (touchoncology.com)
Early features such as stem cell properties has long been proven to be linked to early relapse in AML. (ghrnet.org)
Long-term hematopoietic stem cell damage in a murine model of the hematopoietic syndrome of the acute radiation syndrome. (iu.edu)
Immune-deficient mouse kinds of liver damage allow examination of human being stem cell migration to sites of damage and subsequent contribution to repair and survival. (woofahs.com)
In addition, it appears possible that alternative systems, 87976-03-2 supplier such as the induction of angiogenic procedures stem-cell-mediated and [20] improvement of mobile growth [10], are included in the cumulative benefit of stem-cell-mediated restoration of nonhematopoietic body organs. (woofahs.com)
Blood-derived endothelial progenitor cells (EPCs) represent the "promoters" of vascular repair providing the rationale for autologous stem cell therapy [ 2 ]. (biomedcentral.com)
One limitation of this study was the lack of highly purified immature progenitor and stem cell populations which precluded dissection of the very first transcriptional events linked to commitment. (biopaqc.com)
Mutations in TET2, other epigenetic regulators, and other regulators of cytokine signaling are not specific to the classic myeloproliferative neoplasms (MPNs) but may influence prognosis and play roles in hematopoietic stem cell (HSC) dysregulation and progression to accelerated or blast-phase disease. (oncohemakey.com)
While quadruplet induction therapies deepen responses in newly diagnosed multiple myeloma patients, their impact on peripheral blood stem cell (PBSC) collection remains incompletely understood. (biomedcentral.com)
This study demonstrates that stem cell collection is feasible after prolonged induction with isatuximab-RVd without collection failures and might be further explored as induction therapy. (biomedcentral.com)
However, during stem cell mobilization and -collection, no study-specific therapeutic intervention was performed. (biomedcentral.com)
Therefore, to treat cancer effectively, the cancer stem cell must be eliminated. (hoajonline.com)
Gene delivery through stem cells using specific viral vectors are also utilised to confer lineage specificity of transducedautologous or allogeneic stem cell gene expressionin the recipient while addressing graft versus host disease. (hoajonline.com)
The best hope for regeneration of these permanently damaged cells is optic stem cell therapy combined with continued drug treatments or other exogenous biomaterial implants depending upon the relevant ophthalmological condition. (hoajonline.com)
Stem cell (SC) therapy is not a new concept. (biomedcentral.com)
In the field of stem cells, cumulative evidence has shown that mitochondrial transfer contributes to stem cell-triggered repair of damaged cells [ 9 ]. (biomedcentral.com)
In this study we asked if MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if artificial cultivation conditions exert any influence on their stem cell maintenance. (biomedcentral.com)
The Hippo pathway also has a critical role in stem cell and tissue specific progenitor cell self-renewal and expansion. (hippoinhibitors.com)

Origin is the pathological proliferation of clonal myeloid cells, mostly belonging to the highly proliferative progenitor pool (i.e. (altmeyers.org)
While progenitor cells can divide and replicate, their proliferation potential is more restricted compared to stem cells. (thenoveldifference.com)
Acute myeloid leukemia (AML) results from the over-proliferation of progenitor cells of the myeloid lineage in the bone marrow. (ghrnet.org)
PMF is characterized by proliferation predominantly of bone marrow megakaryocytes and granulocytes, resulting in deposition of fibrous connective tissue, and often associated with peripheral blood leukoerythroblastosis, cytopenias, and constitutional symptoms. (oncohemakey.com)
FLT3 (FMS-related tyrosine kinase 3) located on chromosome 13q12.2 encodes a receptor tyrosine kinase (RTK) that activates the Ras and PI3 kinase pathway leading to the increased proliferation and inhibition of apoptosis in hemopoietic progenitor cells [ 7 ]. (hindawi.com)
B-cell disorders are divided into defects of B-cell development/immunoglobulin production ( immunodeficiencies ) and excessive/uncontrolled proliferation ( lymphomas , leukemias ). (medscape.com)
Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. (thermofisher.com)
The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. (thermofisher.com)
These results indicate that proliferation and self-renewal ability of CRC SCs are reduced in HOXA4 and HOXA9 knockdown cells. (stemcellsciencenews.com)
UCHL1 depletion in SF188 and SJ-GBM2 glioma cells was associated with decreased cell proliferation and invasion, along with a reduced ability to grow in soft agar and to form spheres. (stemcellsciencenews.com)
Genetic silencing of TEAD1 suppressed multiple malignant phenotypes of OS cells including cell proliferation, apoptosis resistance, and invasive potential. (stemcellsciencenews.com)
ALL is a malignant clonal proliferation of lymphoid progenitor cells, most commonly of the B-cell lineage (B-ALL). (biomedcentral.com)

The generation of receptors specific for antigens is a unique and complex process that generates 10 12 specific receptors for each cell type of the adaptive immune system, including T and B cells. (medscape.com)
Various cell types can process and present these antigens to T cells, or antigens may be soluble and bound to B-cell receptors. (medscape.com)
The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. (thermofisher.com)
CAR-expression on T or NK cells allows them to specifically target cancer cells via recognition of tumor associated antigens. (nature.com)
The addition of separate adapter molecules (AMs) specific for tumor antigens and CAR-immune cells targeting these AMs allows a more precise and temporally limited therapy. (nature.com)
Furthermore, T cells and NK cells complement each other in that certain immune suppression mechanisms taken by tumor cells that are effective against T cells, such as the downregulation of human leukocyte antigens (HLA), have proven to be stimulatory for NK cells. (molcells.org)

It may also mediate the attachment of hematopoietic stem cells to bone marrow extracellular matrix or directly to stromal cells. (wikipedia.org)
Furthermore, we show expression of MSTN by CML mesenchymal stromal cells, and that myostatin propeptide has a direct and instant effect on CML cells, independent of myostatin, by demonstrating binding of myostatin propeptide to the cell surface and increased phosphorylation of STAT5 and SMAD2/3. (haematologica.org)
MyeloCult™ media have been developed for the long-term culture of hematopoietic progenitor cells in association with adherent stromal cells. (stemcell.com)
Abnormal DNA methylation modifies HOX genes expression in bone marrow mesenchymal stromal cells of myelodysplasias and de novo acute myeloid leukemias. (lnox-team.org)

They produce a defined number of specialized cells and do not have the same capacity for self-renewal as stem cells. (thenoveldifference.com)
They are thought to be infrequent among the tumour cells, quiescent and able to maintain their population by self-renewal. (touchoncology.com)
In the apex of the hierarchy are hematopoietic stem cells (HSC) which are capable of self-renewal have multi-lineage potential and are responsible for generating all the lineages of hematopoietic cells in the blood. (biopaqc.com)
HSC self-renewal capacity and multipotentiality are gradually lost as cells progress through numerous multi- oligo- and uni-lineage intermediates eventually DZNep acquiring either erythroid myeloid or lymphoid identity. (biopaqc.com)
Maturation of the terminally differentiated cell loses the phenotype of the self-renewal antigen (eg. (hoajonline.com)
All stem cells are undifferentiated cells that exhibit unlimited self-renewal and can generate multiple cell lineages or more restricted progenitor populations that can contribute to tissue homeostasis by replenishing the cells or to tissue regeneration after injury. (biomedcentral.com)

These progenitor cells have greater clonal expansion and proliferative capacity than normal bone marrow cells. (sajbl.org.za)
1 The BCR-ABL1 fusion protein is a constitutively active tyrosine kinase and triggers a cascade of aberrant downstream signaling pathways leading to clonal outgrowth of CML cells and subsequent disease manifestation. (haematologica.org)
The tumour cells are malignant plasma cells of the same clonal origin, and secrete high amounts of monoclonal antibody, known as the M-component. (touchoncology.com)
The chronic myeloproliferative neoplasms (MPNs) are clonal disorders characterized by overproduction of mature myeloid cells. (oncohemakey.com)
The MPNs comprise a set of clonal HSC disorders, characterized by the overproduction of 1 or more mature myeloid cell types. (oncohemakey.com)

VEGFR1-positive haematopoietic bone marrow progenitors initiate the pre-metastatic niche. (southernbiotech.com)

Focusing on novel positive regulators of primitive CML cells, the myostatin antagonist myostatin propeptide gave the largest increase in cell expansion and was chosen for further studies. (haematologica.org)
Herein, we demonstrate that myostatin propeptide expands primitive CML and normal BM cells, as shown by increased colony-forming capacity. (haematologica.org)
In summary, we identify myostatin propeptide as a novel positive regulator of primitive CML cells and corresponding normal hematopoietic cells. (haematologica.org)
2 1 There is growing evidence to suggest that primitive CML cells affect the bone marrow (BM) niche, contributing to deregulated cytokine levels. (haematologica.org)
Thrombopoietin promotes adhesion of primitive human hemopoietic cells to fibronectin and vascular cell adhesion molecule-1: role of activation of very late antigen (VLA)-4 and VLA-5. (ox.ac.uk)
Finally, Tpo stimulated adhesion of primitive (CD34+ CD38-) human bone marrow cells to fibronectin, predominantly through activation of VLA-5, whereas no such effect could be observed on CD34+ CD38+ bone marrow cells. (ox.ac.uk)
Comparing the cell surface expression on NPM1-mutated AML cells to primitive (CD34+ CD38-) normal bone marrow cells, we identified the complement receptor C3AR as specifically expressed in NPM1-mutated AML. (lu.se)

i.e., they are multipotent hematopoietic stem cells. (wikipedia.org)
Derived from the fetal allantois, it provides a rich source of multipotent stem cells, including CD34+ and CD38- haematopoietic progenitor cells. (sajbl.org.za)
These studies support the lineage priming hypothesis which argues that multipotent progenitors communicate before lineage restriction low levels of genes known to be important determinants of unique fates 11. (biopaqc.com)
In the cellular level earlier models of hematopoietic commitment described a unique binary break up between myeloid and lymphoid fates immediately downstream of DZNep a multipotent cell 12. (biopaqc.com)

Hematopoiesis is the process of blood cell formation. (thenoveldifference.com)
The cell-adhesive laminins affected migration of hematopoietic progenitors, suggesting a physiologic role for laminins during hematopoiesis. (ox.ac.uk)
Inhibition of hematopoiesis results in reduced populations of blood forming cells. (hoajonline.com)
In the after-math of the bombings of Hiroshima and Nagasaki in 1945, researchers discovered that bone marrow (BM) transplanted into irradiated mice produced hematopoiesis [ 1 ]. (biomedcentral.com)

Thus, Tpo might play an important role in early hemopoiesis, at least in part through its ability to promote adhesion through activation of adhesion molecules on hemopoietic progenitor cells. (ox.ac.uk)

Acute myeloid leukemia (AML) is a neoplasm of myelopoiesis with variable involvement of myeloid cell lineages. (altmeyers.org)
Hematopoietic stem cells give rise to progenitor cells, which, in turn, differentiate into specific blood cell lineages. (thenoveldifference.com)

The CD38 molecule, with its high and homogenous expression on Multiple Myeloma (MM) cells, appears a suitable target for antibody therapy. (biomedcentral.com)
Investigators showed that SL-401 also decreases the viability of interleukin-3R-expressing cancer stem-like cells in multiple myeloma. (stemcellsciencenews.com)
In this review, recent advances achieved in the characterization of cancer stem cells derived from hematological malignancies, with a particular focus on multiple myeloma, are discussed and major natural products that target cancer stem cells are presented. (stemcellsciencenews.com)
The Food and Drug Administration granted accelerated approval to talquetamab-tgvs (Talvey, Janssen Biotech, Inc.) for adults with relapsed or refractory multiple myeloma who have received at least four prior lines of therapy, including a proteasome inhibitor, an immunomodulatory agent, and an anti-CD38 monoclonal antibody. (cancerfax.com)
Monoclonal antibody that binds with high affinity to the CD38 molecule, which is highly expressed on the surface of multiple myeloma cells. (medscape.com)

Also in a xenotransplant model, i.v. injected CD38-CAR T cells were effective against MM tumors growing in a human bone marrow-like microenvironment, thus demonstrating their ability to properly migrate and infiltrate into the tumor niche to lyse malignant cells. (biomedcentral.com)
Cancer stem cells (CSCs) are generated by a combination of DNA mutations, epigenetic events, and tissue microenvironment factors from normal stem cells or precursor/progenitor cells, to which they are closely related and share many of the behaviors and features 1 . (atlasantibodies.com)
By stimulating the production of fat cells in the bone marrow and adjusting the bone marrow microenvironment, it can inhibit leukemia cells and stimulate normal blood cell production. (cancerfax.com)
By changing the bone marrow microenvironment, it provides vitality for healthy blood cell production and at the same time inhibits the formation of leukemia cells, which may provide a new way of indirect treatment for acute myeloid leukemia. (cancerfax.com)

Human embryonic stem cells (hESCs) derived from an early embryo can self-renew indefinitely and differentiate into a variety of cell types. (ashpublications.org)
Although less controversial than the use of embryonic stem cells obtained from fetal tissue, the practice of UCB biobanking presents several ethical and regulatory challenges surrounding its procurement and use, especially in developing countries like South Africa, where the majority of the population is vulnerable and prone to exploitation. (sajbl.org.za)
As a source of non-embryonic stem cells, UCB has found potential uses as a therapeutic modality in more than 80 clinical applications, both haematological and non-haematological. (sajbl.org.za)
They are commonly expressed in pluripotent embryonic stem cells, germ cells, certain committed progenitors, and cancer cells 18 . (atlasantibodies.com)
Embryonic stem cells which are characteristic of totipotency differentiate into generations of the multiple organs. (hoajonline.com)

In the present study we demonstrate that Tpo can stimulate the adhesion of the Mo7e progenitor cell line to fibronectin (Fn) as well as vascular cell adhesion molecule-1 through activation of very late antigen (VLA)-4 and VLA-5, adhesion molecules previously demonstrated to be involved in regulation of steady state hemopoiesis. (ox.ac.uk)
His Immunology study deals with Cytotoxic T cell intersecting with Antigen and T cell. (research.com)
Binding of complement to a foreign substance, or antigen, amplifies and augments the body's innate immune system by means of its role as an opsonin (a factor that enhances phagocytosis of unwanted particles) and as a chemoattractant (a factor that recruits cells to areas of inflammation). (medscape.com)
Adoptive transfer of T cells transduced with tumor-reactive Chimeric Antigen Receptors (CARs) is a promising strategy for cancer immunotherapy. (biomedcentral.com)
Both continue their development in the bone marrow through an antigen-independent process called primary lymphopoiesis (PL). Recognized stages of PL are pro-B cell, pre-B cell, immature B cell, and mature B cell. (medscape.com)
Secondary lymphopoiesis (SL) begins when mature B cells enter the extrafollicular area of lymphoid tissue and differentiate into short-lived plasma cells and memory cells after being stimulated by antigen-presenting cells. (medscape.com)
Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. (thermofisher.com)
Chimeric antigen receptor (CAR)-T cell therapies are on the verge of becoming powerful immunotherapeutic tools for combating hematological diseases confronted with pressing medical needs. (nature.com)
Besides modification based on a second-generation CAR, more advanced CAR-immune cell therapeutics are being tested, which utilize precise insertion of genes to circumvent graft-versus-host disease (GvHD) or employ a dual targeting approach and adapter CARs in order to avoid therapy resistance caused by antigen loss. (nature.com)
One way to achieve this is to genetically modify immune cells, mainly T cells and recently also natural killer (NK) cells, to express chimeric antigen receptors (CARs). (nature.com)
Binding of a tumor antigen via the scFv activates the T cell in a major histocompatibility-independent manner which leads to a cytotoxic response [ 3 ]. (nature.com)
Furthermore, the so-called adapter CARs have been developed by splitting antigen recognition and CAR-immune cell activation. (nature.com)

They differentiate into very specific cells. (thenoveldifference.com)
HSCs have the remarkable ability to differentiate into a wide range of specialized blood cells. (thenoveldifference.com)
Memory cells travel to the primary follicle, where, after exposure to dendritic cells, they differentiate into centroblasts (immunoglobulin class-switch). (medscape.com)
Centroblasts progress to centrocytes with high-affinity antibody production, then differentiate further to long-term memory cells and plasmablasts. (medscape.com)
The totipotentstem cells differentiate further into pluripotency thatpopulate the organs with different sub-divisional components of the various individual organs. (hoajonline.com)
mesenchymal stem cells - placenta) differentiate into multipotency as precursors to formation of the cellular components. (hoajonline.com)
Hematopoietic stem cells (HSCs) were first identified in 1961, and their ability to migrate and differentiate into multiple cell types was documented [ 2 ]. (biomedcentral.com)
A progenitor cell is a biological cell that, like an SC, has a tendency to differentiate into a specific type of cell but is already more specific than an SC and is pushed to differentiate into its 'target' cell. (biomedcentral.com)
There already exist several protocols to genetically modify and differentiate iPSCs into NK cells, and each has its own advantages with regards to immunotherapies. (molcells.org)
Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. (biomedcentral.com)

In addition, this cell population might be a novel therapeutic target for AML. (researchsquare.com)
In view of the fact that UCB has become a valuable, non-invasive source of stem cells for regenerative therapy, establishment of a public cord blood bank (CBB) in South Africa would vastly improve the availability of haematopoietic stem cells for research and therapeutic uses, and increase the tissue genetic diversity that currently impedes the South African bone marrow registry. (sajbl.org.za)
Progenitor cells are being explored for various therapeutic applications, including tissue engineering and regenerative medicine. (thenoveldifference.com)
Thus, umbilical cord blood EPCs hold great therapeutic potential for cell therapy and vascular engineering. (biomedcentral.com)
These results signify the potential importance of CD38-CAR T cells as therapeutic tools for CD38 + malignancies, including MM, and warrant further safety and efficacy evaluation in appropriate models. (biomedcentral.com)
Finally, we show that antibodies directed against C3AR efficiently elicit NK cell-mediated killing of primary AML cells ex vivo, highlighting C3AR as a candidate therapeutic target in NPM1-mutated AML. (lu.se)
Therapeutic regeneration of molecularly engineered and transplanted autologous stem cells in neurological and cardiovascular diseases due to inability of self-regeneration or repair of endogenous stem cells at sites of inflammation or disease at various stages of progress for regeneration of the damaged tissue depending upon the sites and nature of degeneration. (hoajonline.com)
Cancer stem cellsand their susceptibility over their expected to be "downstream or progeny" cancer cells represent a target for therapeutic interventions to prevent latency and consequently relapse or metastasis of the malignant disease. (hoajonline.com)
According to recent findings, especially targeting cancer stem cells (CSCs) is the most promising therapeutic strategy. (bmbreports.org)
Lately, CAR-NK cell therapies have also come into focus as novel therapeutic options to address hurdles related to CAR-T cell therapies, such as therapy-induced side effects. (nature.com)
Prior to infusion of the CAR-modified immune cells, lymphodepletion is performed in most therapeutic settings to allow efficient cell engraftment [ 10 ]. (nature.com)
With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. (biomedcentral.com)
In recent years mesenchymal stem cells (MSC) have generated a great deal of interest as a potential source for cell-based therapeutic strategies. (biomedcentral.com)
The researchers pointed out that the focus of the existing standard treatment is to kill tumor cells, change the way of thinking, and adopt different strategies to change the survival environment of cancer cells to achieve therapeutic effects. (cancerfax.com)

Most CAR-T cell-based gene therapy products that are under clinical evaluation consist of autologous enriched T cells, whereas CAR-NK cell-based approaches can be generated from allogeneic donors. (nature.com)
An autologous CAR-T or NK cell therapy comprises several steps as shown in Fig. 1 . (nature.com)

Studies in murine models, as well as in human samples, have demonstrated that SALL4 is essential for leukemic cell survival and that SALL4 transgenic mice develop an MDS-like phenotype prior to transformation to AML, suggesting that SALL4 can be a driver of MDS/AML pathogenesis. (confex.com)
His work in Phenotype covers topics such as Regulation of gene expression which are related to areas like Cell biology. (research.com)
Dissociation between mature phenotype and impaired transmigration in dendritic cells from heparanase-deficient mice. (southernbiotech.com)

B and T cells, type 2 dendritic cells, and natural killer (NK) cells share a common ancestor: common lymphoid progenitor (CLP). (medscape.com)

as a cell surface glycoprotein and functions as a cell-cell adhesion factor. (wikipedia.org)
Unlike the ability of these cytokines to synergistically enhance growth of Mo7e as well as normal progenitor cells, no synergy was observed with regard to their ability to enhance adhesion. (ox.ac.uk)
RNA-seq of mobilised CD34+ cells from 21 patients showed that adhesion genes are overexpressed in CD34+ cells after dara-VCd/isa-KRd and JCAD, NRP2, MDK, ITGA3 and CLEC3B were identified as potential target genes. (bvsalud.org)
These findings suggest that upregulated adhesion-related interactions, rather than killing of CD34+ cells by effector mechanisms, could be leading causes of decreased mobilisation efficacy in MM patients treated with anti-CD38 therapy. (bvsalud.org)
Laminin isoform-specific promotion of adhesion and migration of human bone marrow progenitor cells. (ox.ac.uk)
We studied human bone marrow cell adhesion to laminin-10/11 (alpha5beta1gamma1/alpha5beta2gamma1), laminin-8 (alpha4beta1gamma1), laminin-1 (alpha1beta1gamma1), and fibronectin. (ox.ac.uk)
The MEC13.3 antibody specifically recognizes CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1). (bdbiosciences.com)
CD31 is a 130 kDa integral membrane protein, a member of the immunoglobulin superfamily, that mediates cell-to-cell adhesion. (bdbiosciences.com)
Benefits corroborated earlier types which may have shown within vitro, the highest performance with the Lol and SPCL scaffolds about supporting cells adhesion along with growth. (hippoinhibitors.com)

Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
Laminins containing alpha4 and alpha5 chains are expressed in bone marrow, but their interactions with hematopoietic progenitors are unknown. (ox.ac.uk)

Flow cytometry analysis of CD19 was performed by staining Raji CD19 Knock out cells with 0.25 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ (Product # 12-4714-82, yellow histogram) or 0.25 µg CD19 Monoclonal Antibody (HIB19), PE, eBioscience™ (Product # 12-0199-42, blue histogram). (thermofisher.com)
Classical " CARs consist of an extracellular binding domain mostly derived from a monoclonal antibody fragment (single-chain variable fragment-scFv), which is linked to intracellular binding domains of the T-cell receptor complex. (nature.com)

The complexity of the human bone marrow, with context to haematological malignancies, is more difficult to elucidate with the present strategies compared to the niche of solid tumors. (fortunejournals.com)
Thus homeostasis of T regulatory cells is an overbearing requirement for prevention of autoimmune disorders arising from uncontrolled self-tolerance which also permits malignancies to occur. (hoajonline.com)
In order to examine the role of HDAC9 in the lymphoid development and pathogenesis of lymphoid malignancies we used Ig heavy chain enhancer (Eμ), which drives gene expression from early stages of B-cell development, to ectopically express HDAC9 in transgenic mice. (sunderland.ac.uk)

Injection of CD34+ hematopoietic stem cells has been clinically applied to treat various diseases including spinal cord injury, liver cirrhosis and peripheral vascular disease. (wikipedia.org)
2 , 3 Normally, the bone marrow serves as the source for replenishing the cellular components of peripheral blood including red and white blood cells and platelets. (sajbl.org.za)
EPCs from human umbilical cord and adult peripheral blood activate different mechanisms upon high-dose x-ray radiation treatment: CB-EPCs undergo p53 stabilization, Bax-dependent apoptosis and p21-dependent G 1 and G 2 /M cell cycle checkpoints, while PB-EPCs undergo only radiation-induced senescence [ 13 ], indicating unique gene expression patterns in EPCs of different sources. (biomedcentral.com)
Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. (thermofisher.com)
The morphological examination of peripheral blood or bone marrow smears, although still an indispensable part of routine laboratory testing, is clearly insufficient for patient management, and clinicians should not ask themselves whether to look for MRD or not, but how and when. (smw.ch)
Use of peripheral blood instead of bone marrow to monitor residual disease in children with acute lymphoblastic leukemia. (smw.ch)

They are committed to a specific cell lineage but retain the ability to generate a limited range of related cell types, making them essential for tissue repair, regeneration, and potential medical interventions. (thenoveldifference.com)
We evaluated the expression of SALL4 using single-cell mass cytometry (CyTOF) utilizing 28 antibodies including surface lineage markers and intracellular proteins, such as p53, ki67, c-myc and pAKT to identify SALL4 expressed cells and related pathway in bone marrow (BM) for 10 MDS patients. (confex.com)
In mouse several studies have explained the manifestation of lineage-affiliated transcriptional programs within multilineage progenitors 8-10. (biopaqc.com)
The recognition of MLP provides a unique opportunity to investigate the molecular mechanisms underlying lymphoid vs myeloid lineage choice in main human being hematopoietic cells. (biopaqc.com)
Laminin-10/11 was highly adhesive to lineage-committed myelomonocytic and erythroid progenitor cells and most lymphoid and myeloid cell lines studied, whereas laminin-8 was less adhesive. (ox.ac.uk)
CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. (thermofisher.com)

Leukemia stem cells (LSCs) in acute myeloid leukemia (AML) played important roles in development of leukemia, chemotherapeutic drug resistance, and disease relapse and progression. (researchsquare.com)
PEGylated G-CSF (BBT-015), GM-CSF (BBT-007), and IL-11 (BBT-059) analogs enhance survival and hematopoietic cell recovery in a mouse model of the hematopoietic syndrome of the acute radiation syndrome. (iu.edu)
They also lysed primary malignant cells from acute myeloid leukemia, and multi-drug resistant MM patients. (biomedcentral.com)
Although these disorders share certain clinical features-including bone marrow hypercellularity, frequent splenomegaly, and risks of thrombosis, hemorrhage, and transformation to acute myeloid leukemia (AML)-they also show important phenotypic differences. (oncohemakey.com)
Acute (AML) and chronic (CML) myeloid leukemia are cancers of the myeloid line of blood cells, characterized by the rapid growth of abnormal cells that build up in the bone marrow and blood and interfere with normal blood cell production. (atlasantibodies.com)
Acute myeloid leukemia (AML) is characterized by the generation of heterogeneous leukemia cells. (cancerfax.com)

Progenitor cells are often located within specific tissues, close to where they will ultimately function. (thenoveldifference.com)
They offer the potential to replace damaged or dysfunctional cells in specific tissues or organs. (thenoveldifference.com)
Among all cancerous cells, a few act as stem cells that reproduce and sustain the tumor, much like stem cells normally renew and sustain our organs and tissues. (atlasantibodies.com)
Hematological cancers (blood cancers) arise in the blood-forming tissues, such as bone marrow, lymph nodes, and lymphatic system. (atlasantibodies.com)
Among the known HIF-1 target genes are those encoding erythropoietin(EPO) and vascular endothelial growth factor (VEGF), which promote oxygen delivery to tissues by stimulatingthe production of red blood cells and blood vessels, respectively. (grantome.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
How do mesenchymal stem cells from different tissues differ in terms of their biological function? (promocell.com)

Other cellular components of the innate immune system include mononuclear phagocytes, dendritic cells, and NK cells. (medscape.com)
There were no substantial variations involving bone marrow and cord blood HSC with regards to the frequency of generation of CD4+HLADR+ dendritic cells. (idhinhibitor.com)
Follicular dendritic cells also express CD19. (thermofisher.com)

Cytokines are essential for the function and maintenance of cells, and altered cytokine levels influence not only leukemic cells, but also the normal HSC within the BM. (haematologica.org)

We generated three retroviral CAR constructs based on huCD38 antibodies, CD3ζ and 4-1BB signaling domains and transduced them into T cells of healthy donors and MM patients to test the in vitro and in vivo efficacy. (biomedcentral.com)
In vitro functional clonogenic assays for IL-7 responsive BM-derived B-cell progenitors demonstrated an increase (∼50%) in colony numbers in the transgenic BM. (sunderland.ac.uk)
The present research was designed to look into the effect of mixing fresh dexamethasone-loaded carboxymethylchitosan/poly(amidoamine) dendrimer (Dex-loaded CMCht/PAMAM) nanoparticles and, both ' and also SPCL scaffolds (3 dimensional technique) for the spreading and also osteogenic difference associated with rat bone tissue marrow stromal cellular material (RBMSCs) inside vitro. (hippoinhibitors.com)
In vitro experiments, PPARγ inhibitors can induce the generation of bone marrow adipocytes. (cancerfax.com)
Kay et al report increased VEGF and bFGF in the supernatant of CLL cells grown in vitro and upregulation of mRNA encoding VEGF and its receptors and bFGF, suggesting that angiogenic factors are important in the biology of the malignant B-cell clone [ 10 ]. (biomedcentral.com)
elicits antiproliferative and proapoptotic activities in vitro in solid and hematologic tumor cells. (medscape.com)

Thus, because of their CD34+ expression, such undifferentiated cells can be sorted out. (wikipedia.org)
However, SALL4 expression status and related pathway in bone marrow (BM) cells and relationship with somatic gene mutations in MDS patients has not been explored. (confex.com)
In this study, we evaluated the expression of SALL4 and related factors using single-cell mass cytometry (CyTOF) and NanoString technology in MDS patients alongside with target sequence for MDS-related mutations. (confex.com)
Conversely only 3 MDS patient had weak SALL4 expression in CD3+ T cells. (confex.com)
Next, we also assessed an expression level of SALL4A and SALL4A&B in 14 MDS patient bone marrow (BM) samples using Nanostring nCounter. (confex.com)
His work carried out in the field of Molecular biology brings together such families of science as Cell culture and Gene expression. (research.com)
Aberrant expression of a third marker such as Thy-1/CD90 negativity, expression of CLL-1 and IL-3R (CD123), intermediate levels of aldehyde dehydrogenase and co-expression of common chromosomal translocation may be used to distinguish from normal hematopoietic stem cells. (ghrnet.org)
Irrespective of the donor, CD38-CAR T cells lost CD38 expression, expanded readily and lysed MM and other malignant cell lines in a cell dose-, and CD38-dependent manner. (biomedcentral.com)
Initial gene expression analysis on 38 human being hematopoietic cell subtypes recognized gene modules and transcription factors circuits active in stem and progenitor cell-enriched fractions and reused HIRS-1 in terminally differentiated cells 7. (biopaqc.com)
By flow cytometry and single cell RNA-sequencing, we further show that normal hematopoietic stem and progenitor cells lack detectable C3AR gene and protein expression, making it particularly suitable as a target for antibody therapy. (lu.se)
Two-color flow cytometric analysis of CD31 expression on BALC/c mouse bone marrow cells. (bdbiosciences.com)
Two-color flow cytometric dot plots show the correlated expression patterns of CD31 (or Ig Isotype control staining) versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. (bdbiosciences.com)
Identification of PARD3 signature on PARD3 deficient H157 cell line, reconstituting the expression of PARD3 gene, with a wt and a mutant form. (nih.gov)
Decreased histone acetylation is a hallmark of cancer cells and increased HDAC expression or their mistargetting to specific gene promoters has been associated with a variety of tumors. (sunderland.ac.uk)
Taken together these data suggest that HDAC9 plays a role in B-cell maturation and its ectopic expression in early B-cells leads to perturbation of normal B-cell development, possibly predisposing transgenic mice to tumorigenesis. (sunderland.ac.uk)

In the xenotransplantation versions with individual cells being injected into rodents, the contribution from the donor individual cells to the broken liver organ can end up being conveniently noticed by human-specific indicators or centromeric chromosome evaluation by Seafood that discriminate between murine and individual chromosomes (Fig.?1) [22, 23]. (woofahs.com)
cells engraft in beige/nude/xid immune-deficient mice and their progeny can be recovered from the murine bone marrow for as long as 18?months posttransplantation [28, 29]. (woofahs.com)
Indeed, Tie1-positive tumor cells derived from a murine model overexpressed Lgr5, a typical stemness marker for colorectal cancer. (stemcellsciencenews.com)

The use of CD133 (PROM1) to identify CSCs in glioma is still in question because tumorigenic cells are equally found in both CD133+ and CD133− cells in some gliomas, while some CD133+ brain tumor cells may not possess tumor-initiating capacity 23 . (atlasantibodies.com)
In marked contrast to the cancer stem cells, the tumor cells without "stemness" have either no capacity or a markedly diminished capacity, to form new tumors. (hoajonline.com)
Analysis using sphere-formation culture revealed that Tie receptor 1 (Tie1)-positive cells are enriched within the population of tumor cells with cancer stemness properties. (stemcellsciencenews.com)
Unlike primary NK cells, those prepared from iPSCs can be prepared with a homogeneous quality and are easily modified to exert a desired response to tumor cells. (molcells.org)

For lung cancers, CSC markers include CD44 and CD133 (PROM1), but also CD117 (KIT), CD90 (or THY1), CD166, EpCAM for non-small-cell lung carcinoma (NSCLC), and PODXL-1, PTCH, and CD87 for small-cell lung carcinoma (SCLC) 25 . (atlasantibodies.com)

However, counting CD34+ mononuclear cells may overestimate myeloid blasts in bone marrow smears due to hematogones (B lymphocyte precursors) and CD34+ megakaryocytes. (wikipedia.org)

In this review, we are going to take a closer look at the commercial CAR-T cell therapies, as well as on CAR-T and CAR-NK cell products, which are currently under evaluation in clinical trials, that are being conducted in Germany. (nature.com)
However, their low frequency in bone marrow necessitate ex vivo expansion for further clinical application. (biomedcentral.com)
Clinical suspicion for ALL arises with signs and symptoms reflective of bone marrow failure (pancytopenia) and/or extramedullary disease. (biomedcentral.com)

Following B-cell receptor activation, 2 waves of tyrosine kinase phosphorylation occur. (medscape.com)
X-linked agammaglobulinemia (XLA), also known as Bruton agammaglobulinemia, results from a mutation of the BTK gene, which encodes the pre-B-cell receptor (Pre-BCR) and BCR. (medscape.com)
Using a 3D fibrin gel model, researchers showed that IFN-γ induces tumor-repopulating cells to enter dormancy through an indolamine 2,3-dioxygenase 1 (IDO1)-kynurenine-aryl hydrocarbon receptor (AhR)-p27 dependent pathway. (stemcellsciencenews.com)

In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)

The study revealed for the first time this relationship between the generation of adipocytes in the bone marrow and normal bone marrow erythrocytes. (cancerfax.com)

Aberrantly expressed cytokines in the bone marrow (BM) niche are increasingly recognized as critical mediators of survival and expansion of leukemic stem cells. (haematologica.org)
Out of the 313 unique human cytokines evaluated, 11 were found to expand cell numbers ≥2-fold in a 7-day culture. (haematologica.org)
Natural-killer (NK) cells are specialized lymphocytes that have cytotoxic properties in addition to their ability to produce cytokines that assist in the orchestration of adaptive immunity. (medscape.com)

Progenitor cells play a crucial role in tissue repair and regeneration. (thenoveldifference.com)
When specific tissue or organ damage occurs, these cells can rapidly generate new cells of the desired type to facilitate the healing process. (thenoveldifference.com)
Like organ tissue stem cells, CSCs are thought to be rare and to enter the cell cycle infrequently. (touchoncology.com)
The existence of stem cells in hematological tissue prompted research in other tissue cancer types. (atlasantibodies.com)
The more aggressive forms of hematopoietic and lymphoid tissue diseases require treatment with chemotherapy, radiotherapy, immunotherapy and, in some cases, a bone marrow transplant. (atlasantibodies.com)
One great advantage of MSC is that these cells may be directly obtained from individual patients, thereby eliminating the complications associated with immune rejection of allogenic tissue and infectious diseases. (biomedcentral.com)
The human MSC derived from bone marrow, adipose tissue, and umbilical cord matrix are from different origins, but with comparable biological properties and function. (promocell.com)

We possess proven that, although rare relatively, cell blend will can be found in between transplanted individual control cells and receiver immune-deficient mouse hepatocytes vivo, ending in individual albumin-expressing cells [19]. (woofahs.com)

Here, they reside in specialized niches where they remain quiescent until needed to replenish and repair the blood cell population. (thenoveldifference.com)

Normally, potentially malignant cells are continuously eliminated by the immune system, but cancer cells can accumulate certain mutations, which allow them to escape these mechanisms [ 2 ]. (nature.com)
CAR-modified immune cells are expanded until sufficient cell numbers are attained and are adoptively transferred into the patient to fight malignant cells. (nature.com)
Minimal residual disease (MRD) refers to the small number of malignant cells that remain after therapy when the patient is in remission and shows no symptoms or overt signs of disease. (smw.ch)

As shown in Table 2, the frequency of bone marrow HSC and cord blood HSC differentiating into CD34+ HSC and CD14+ HLA-DR+ monocytes didn't differ substantially. (idhinhibitor.com)

There can, however, be few similarities between the two, as the bone marrow is a common site of metastasis of solid tumors too [6]. (fortunejournals.com)
Relevant information has now been established on solid tumors and their micro-environments, which include immune, endothelial and mesenchymal cells. (fortunejournals.com)
One crucial ability of the CSCs is to form tumors already at low cell numbers. (atlasantibodies.com)
The authors established that this CD34+/ CD38− subpopulation was capable of initiating tumors 3 . (atlasantibodies.com)
On the other hand, in brain tumors, such as glioma and glioblastoma, CSCs have instead been identified using cell surface markers, including SSEA-1 20 , EGFR 21 , and CD44 22 . (atlasantibodies.com)
Tumors contain a minority population of cancer stem cells that maintain the tumor. (hoajonline.com)
On the other hand, elimination or escape of Treg cells also causes the reactivation and induction of tumors and relapse. (hoajonline.com)

The earliest thymic progenitors (ETP) and granulocyte-monocyte progenitors (GMP) long thought to be unilineage retained residual myeloid or lymphoid potential respectively 10 13 14 Importantly recent studies in human wire blood and bone marrow shown the living of early lymphoid-biased progenitors that maintain myeloid but not erythroid potential. (biopaqc.com)
In conclusion, we demonstrate laminin isoform-specific adhesive interactions with human bone marrow stem, progenitor, and more differentiated cells. (ox.ac.uk)
Experimental and human studies with intravitreal bone marrow-derived stem cells have begun. (biomedcentral.com)
Scientists report that furin is unique among the proprotein convertases subtilisin/kexin in being highly expressed in human glioma-initiating cells. (stemcellsciencenews.com)
Hemizygous and homozygous mice expressing Flag epitope tagged human HDAC9 (fHDAC9) transgene display throughout their lifespan altered B-cell development. (sunderland.ac.uk)
The discovery of human pluripotent stem cells (PSCs) at the turn of the century opened the door to a new generation of regenerative medicine research. (molcells.org)
Human MSC are easy to isolate from small aspirate of bone marrow via their adherence ability. (biomedcentral.com)

Anatomy55

Organisms14

Diseases24

Chemicals and Drugs80

Analytical, Diagnostic and Therapeutic Techniques and Equipment42

Phenomena and Processes44

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science2

Named Groups1

Health Care1

CD3450

Transplantation16

Differentiation25

Stem cell33

Proliferation12

Antigens6

Stromal cells4

Self-renewal6

Clonal5

Haematopoietic bone marrow1

Primitive7

Multipotent4

Hematopoiesis4

Hemopoietic progenitor cells1

Lineages2

Multiple myeloma5

Microenvironment4

Embryonic5

Antigen12

Differentiate10

Therapeutic14

Autologous2

Phenotype3

Lymphoid progenitor1

Adhesion9

Hematopoietic progenitors2

Monoclonal2

Malignancies3

Peripheral7

Lineage6

Acute6

Tissues7

Dendritic cells3

Leukemic cells1

Vitro6

Expression15

Murine3

Tumor Cells4

Thy11

Megakaryocytes1

Clinical3

Receptor3

Clonogenic1

Normal bone1

Cytokines3

Tissue7

Vivo1

Quiescent1

Malignant cells3

Monocytes1

Tumors7

Human7