Cd27 antibodies. Medical search. Frequent questions

In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. (thermofisher.com)
RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. (thermofisher.com)
CD27 and its ligand CD70 are involved in multiple aspects of T cell biology, including the costimulation and expansion of CD4+ and CD8+ T cells and differentiation to memory CD8+ T cells. (stemcell.com)
CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. (biolegend.com)
CD27 ligand (CD27L), also named CD70, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7 (1, 2). (bio-techne.com)
When CD27 binds CD70, a signaling cascade leads to the differentiation and clonal expansion of T cells. (wikipedia.org)
CD27 binds to CD70 (also known as, CD27 ligand or CD27L) and may be involved in cellular interaction of T and B lymphocytes. (bdbiosciences.com)

High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. (thermofisher.com)
Results Combination treatment with HexaBody-CD27 and all tested anti-PD-(L)1 antibodies enhanced proliferation and proinflammatory cytokine secretion of antigen-specific CD8 + T cells in coculture with cognate antigen-expressing dendritic cells, compared to the respective single-agent treatments. (bmj.com)
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. (thermofisher.com)
Antibodies of the IgG sub-class are bi-functional molecules, possessing a F(ab) domain, variable in sequence and responsible for the binding of antigen, and an Fc domain, constant in sequence and responsible for mediating a range of antibody effector functions [ 1 ]. (bmj.com)
HPV16 IgG antibodies were significantly elevated in the microparticle group compared to antigen solutions administered by the transdermal route. (cdc.gov)

CD27L interacts with CD27, a member of the TNF receptor superfamily that is expressed on natural killer (NK) cells and subsets of T and B cells (2, 4, 5). (bio-techne.com)

This unique mechanism of action distinguishes HexaBody-CD27 from other CD27-targeting monoclonal antibodies. (bmj.com)
Here we review the biology of the FcγRs and IgG isotypes in both humans and mice, detail the potential roles that FcγR interactions can play in the activity of monoclonal antibodies in general, and of immunomodulatory antibodies in particular, and discuss how preclinical studies on these interactions might be best interpreted and translated to a human setting. (bmj.com)

Detects mouse CD27 Ligand/TNFSF7 in direct ELISAs. (bio-techne.com)
Cell Proliferation Induced by CD27 Ligand/TNFSF7 and Neutralization by Mouse CD27 Ligand/TNFSF7 Antibody. (bio-techne.com)
In the presence of sub-optimal amounts of Mouse CD3e Monoclonal Antibody (Catalog # MAB484 ), Recom-binant Mouse CD27 Ligand/ TNFSF7 (Catalog # 783-CL ) stimulates proliferation in mouse splenic T cells in a dose-dependent manner (orange line). (bio-techne.com)
Under these conditions, prolif-eration elicited by Recombinant Mouse CD27 Ligand/TNFSF7 (10 µg/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse CD27 Ligand/ TNFSF7 Monoclonal Antibody (Catalog # MAB783). (bio-techne.com)
Measured by its ability to neutralize CD27 Ligand/TNFSF7-induced proliferation in mouse splenic T cells. (bio-techne.com)
The Neutralization Dose (ND 50 ) is typically 0.5-2 µg/mL in the presence of 10 µg/mL Recombinant Mouse CD27 Ligand/TNFSF7 and sub-optimal amounts of Mouse CD3 epsilon Monoclonal Antibody. (bio-techne.com)

Varlilumab is an IgG1 antibody that binds to CD27 and is an experimental cancer treatment. (wikipedia.org)
This agonist antibody stimulates CD27 when it binds. (wikipedia.org)
The M-T271 monoclonal antibody specifically binds to CD27. (bdbiosciences.com)

dashed line histogram) or FITC Mouse Anti-Human CD27 (Cat. (bdbiosciences.com)

The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. (bdbiosciences.com)
Find CD200R1 Antibodies with a specific conjugate such as PE, FITC, APC. (antibodies-online.com)

Antibody-drug conjugate (ADC) is a new generation of therapeutic drugs, consisting of antibodies, small molecule drugs (payloads), and linkers connecting antibodies and payloads. (genemedi.com)

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD27 Antibody, Clone LG.3A10, followed by a goat anti-hamster (Armenian) IgG antibody, Alexa Fluor® 488 (filled histogram), or an Armenian hamster IgG isotype control antibody, followed by a goat anti-hamster (Armenian) IgG antibody, Alexa Fluor® 488 (solid line histogram). (stemcell.com)
Human peripheral blood lymphocytes were stained with CD27 (clone M-T271) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram). (biolegend.com)
Find CD200R1 Antibodies with a specific Clone. (antibodies-online.com)

Presented preclinical data on KVA12123 and Kineta's anti-CD27 agonist antibody pre-clinical program at the Society for Immunotherapy of Cancer (SITC) 37th Annual Meeting. (tmcnet.com)
Presented preclinical data on Kineta's anti-CD27 agonist antibody pre-clinical program at the AACR Conference on Tumor Immunology and Immunotherapy. (tmcnet.com)

HexaBody ® -CD27 (GEN1053/BNT313) is a novel CD27 human monoclonal antibody with a functionally inert IgG1 Fc domain harboring a hexamerization-enhancing mutation to induce CD27 activation independently of Fc gamma receptor crosslinking, while also avoiding T-cell depletion. (bmj.com)
KVA12123 is a fully human engineered IgG1 monoclonal antibody that was designed to bind to VISTA through a unique epitope at both physiologic and acidic pH levels. (wspa.com)
Flow cytometry analysis of CD19 was performed by staining Raji CD19 Knock out cells with 0.25 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ (Product # 12-4714-82, yellow histogram) or 0.25 µg CD19 Monoclonal Antibody (HIB19), PE, eBioscience™ (Product # 12-0199-42, blue histogram). (thermofisher.com)

Full length human recombinant protein of human CD27 (NP_001233) produced in HEK293T cell. (thermofisher.com)

New model systems are being developed to test and optimize various immunotherapy strategies, including bispecific antibodies, checkpoint inhibitors, epigenetic modifiers and adoptive T cell transfer both in vitro and in vivo. (upenn.edu)
The company's immuno-oncology pipeline includes KVA12123, a novel VISTA blocking immunotherapy currently in a Phase 1/2 clinical trial in patients with advanced solid tumors, and a preclinical monoclonal antibody targeting CD27. (wspa.com)

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. (thermofisher.com)
The LG.3A10 antibody reacts with CD27, a 45 - 55 kDa member of the tumor necrosis factor receptor superfamily. (stemcell.com)
CD27 is a member of the tumor necrosis factor receptor superfamily. (wikipedia.org)

Univariate and multivariate regression analyses showed that low levels of differentiated CD3 + CD27 - CD28 - T cells (23.3 ± 19.3% versus 35.1 ± 18.0%) were independently associated with OR. (frontiersin.org)
In vitro , CD3 + CD8 + CD27 - CD28 - compared to CD3 + CD8 + CD27 + CD28 + CART cells displayed similar CD19 + target cell-specific cytotoxicity, but were hypoproliferative and produced less cytotoxic cytokines (IFN-γ and TNF-α). (frontiersin.org)
Low frequency of differentiated CD3 + CD27 - CD28 - T cells at leukapheresis represents a novel pre-infusion blood biomarker predicting a favorable response to CART cell treatment in r/r DLBCL patients. (frontiersin.org)

All participants will receive the checkpoint inhibitor atezolizumab (Tecentriq) and the experimental drug varlilumab, a novel, first-in-class agonist immunoglobulin G1 anti-CD27 antibody. (medscape.com)

CD27 interacts with TRAF family members, resulting in enhanced cellular proliferation and the induction of anti-apoptotic proteins. (stemcell.com)
Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation (2). (bio-techne.com)
In preclinical studies, HexaBody-CD27 enhanced proliferation and effector functions of activated T cells. (bmj.com)
Methods The effects of combining HexaBody-CD27 with anti-PD-(L)1 antibodies (pembrolizumab, nivolumab, and atezolizumab) on human CD8 + T-cell proliferation and effector functions were investigated in vitro . (bmj.com)

Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. (thermofisher.com)

The effect of combining HexaBody-CD27 with pembrolizumab on IFN-γ secretion was evaluated in a mixed lymphocyte reaction (MLR) assay, with synergy analysis using the Highest Single Agent model. (bmj.com)
In MLR assays of human CD8 + T cells and allogeneic dendritic cells, the combination of HexaBody-CD27 and pembrolizumab synergistically enhanced IFN-γ secretion. (bmj.com)
HexaBody-CD27 is currently being evaluated in patients with advanced solid tumors ( NCT05435339 ). (bmj.com)

The following product was used in this experiment: CD19 Monoclonal Antibody (HIB19), PE, eBioscience™ from Thermo Fisher Scientific, catalog # 12-0199-42, RRID AB_1834376. (thermofisher.com)
Description: The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. (thermofisher.com)

The antibody MEM-57 reacts with gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes. (sigmaaldrich.com)

Fluorescence histograms depicting CD27 (or Ig isotype control) expression were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. (bdbiosciences.com)
An isotype control should be used at the same concentration as the antibody of interest. (bdbiosciences.com)

Other current efforts include the exploration of immunomodulation to potentiate endogenous antitumor T cell responses, use of bispecific antibodies, pharmacological sensitization of tumor cells to immune attack, tumor vasculature targeting, preclinical validations, clinical translation and trial support. (upenn.edu)

B.1.617.2 (Delta) was first isolated in India and also shows some evidence of immune escape, specifically from neutralizing antibodies, but to a lesser degree than B.1.351 7 . (nature.com)
Background Activation of the T-cell co-stimulatory receptor CD27 and blockade of the PD-1:PD-L1 axis can augment antitumor immune responses through distinct mechanisms. (bmj.com)

Abcam: antibodies, proteins, kits. (abcam.com)
We sought to determine if there was antibody deposition in SAH livers and whether antibodies extracted from SAH livers were cross-reactive against both bacterial antigens and human proteins. (elifesciences.org)
Employing human proteome arrays, we profiled the antibodies extracted from explanted SAH, alcoholic cirrhosis (AC), nonalcoholic steatohepatitis (NASH), primary biliary cholangitis (PBC), autoimmune hepatitis (AIH), hepatitis B virus (HBV), hepatitis C virus (HCV) and HD livers and found that antibodies of IgG and IgA isotypes were highly accumulated in SAH and recognized a unique set of human proteins as autoantigens. (elifesciences.org)
This important study tested the hypothesis that liver-derived but not serum-derived antibodies that are cross-reactive to E.coli and to host proteins can play a role in the hepatic damage found in severe alcoholic hepatitis (SAH). (elifesciences.org)
antibodies-online offers a wide range of antibodies, proteins, arrays and kits for cytokines. (antibodies-online.cn)

The following product was used in this experiment: CD27 Monoclonal Antibody (OTI6D6), TrueMAB™ from Thermo Fisher Scientific, catalog # TA812372. (thermofisher.com)

We report characterization of an expanded assay, M9ELISA, that detects antibodies to HPV virus-like particles (VLP) of nine types using direct IgG ELISA on the Meso Scale Discovery (MSD) electrochemiluminescence platform. (cdc.gov)
The method is based on the previously published M4ELISA which detects antibodies to HPV6,11,16, and 18. (cdc.gov)

Malondialdehyde-acetaldehyde adducts and antibody responses in rheumatoid arthritis-associated interstitial lung disease. (cdc.gov)
Gene expression signatures within memory B cells suggest qualitative differences in the antibody responses in aged patients with severe disease. (medrxiv.org)
Type-specific antibody responses to the 9vHPV types were analyzed in 174 first-void urine and 172 serum samples using a virus-like particle-based IgG multiplex enzyme-linked immunosorbent assay. (cdc.gov)

Under this collaboration, Kineta will evaluate the safety, tolerability, pharmacokinetics and anti-tumor activity of KVA12123, its novel anti-VISTA monoclonal antibody, alone and in combination with KEYTRUDA® (pembrolizumab), Merck's anti-PD-1 therapy, in patients with advanced solid tumors. (tmcnet.com)

Additionally, CD27 plays important roles in T cell - B cell interactions and the maturation of effector T cells. (stemcell.com)
Furthermore, CD27 is expressed on both naïve and activated effector T cells as well as NK cells and activated B cells. (wikipedia.org)
Most of these immunomodulatory antibodies are of IgG isotypes that have low, or no, binding to the Fc gamma receptors (FcγRs) that trigger cell-mediated cytotoxic effector functions such as antibody dependent cellular cytotoxicity (ADCC) and phagocytosis (ADCP). (bmj.com)

Opened a Phase 1/2 clinical study evaluating KVA12123, Kineta's novel anti-VISTA monoclonal antibody, in advanced solid tumors, anticipating 10 U.S. sites to participate in this clinical study. (tmcnet.com)

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase. (thermofisher.com)

The antibody was purified by affinity chromatography. (biolegend.com)
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions. (biolegend.com)
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis . (biolegend.com)
Flow cytometric analysis of CD27 on human peripheral blood lymphocytes. (bdbiosciences.com)
Applications Reported: The HIB19 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)

Ig extracted from SAH livers, but not patient serum exhibited hepatocyte killing efficacy in an antibody-dependent cell-mediated cytotoxicity (ADCC) assay. (elifesciences.org)

CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. (thermofisher.com)
Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. (thermofisher.com)
CD27 is found on peripheral T cells, memory B cells, and NK cells and in mice has been found on most thymocytes, except CD46-CD8- precursors. (stemcell.com)
The expansion of activated B cells and their differentiation to plasma cells is also reported to be enhanced by CD27. (stemcell.com)
CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. (biolegend.com)
During mouse embryonic development, specific (medium) expression levels of CD27 (in addition to high cKit, medium Gata2, and high CD31 expression levels) define the very first adult definitive hematopoietic stem cells generated in the aorta-gonad-mesonephros region. (wikipedia.org)
It is a type I transmembrane protein with cysteine-rich domains, but once T cells have become activated, a soluble form of CD27 can be shed. (wikipedia.org)
The CD27 molecule is a lymphocyte-specific member of the TNF/NGF-R family, and is expressed on a subset of human thymocytes and on the majority of mature T lymphocytes, activated B cells and NK cells. (bdbiosciences.com)
CD27 is highly induced on T cells after TCR stimulation. (bdbiosciences.com)
Accordingly, here we analyse antibodies and T cells of a recently vaccinated, UK cohort, alongside those recovering from natural infection in early 2020. (nature.com)
Mouse S1P 5 is a 400 amino acid (aa) 7-transmembrane glycoprotein that is expressed on brain endothelium, renal mesangial cells, Ly6C + (in human CD14 + CD16 - ) monocytes, pre- and mature oligodendrocytes, and CD27 - CD116 + (mature) NK cells. (rndsystems.com)
These isotypes were selected to minimise the risk of depleting the T cells upon which such antibodies depend for their mechanism of action. (bmj.com)

PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse S1P 5 /EDG-8 Monoclonal Antibody (Catalog # MAB9084) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008 ). (rndsystems.com)
HEK293 human embryonic kidney cell line transfected with mouse S1P 5 /EDG-8 and eGFP were stained with and either (A) Rabbit Anti-Mouse S1P 5 /EDG-8 Monoclonal Antibody (Catalog # MAB9084) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C ) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B ). (rndsystems.com)

Find CD200R1 Antibodies validated for a specific application such as WB, ELISA, FACS, IF (cc). (antibodies-online.com)

Background & Aims: During chronic HCV infection, T cell dependent virus-specific antibodies are produced. (eur.nl)

After adjustment for covariates, the highest quartiles of IgA anti-MAA antibody concentration (odds ratio 2.09 [95% confidence interval 1.11-3.90]) and IgM (odds ratio 2.23 [95% confidence interval 1.19-4.15]) were significantly associated with the presence of RA-ILD. (cdc.gov)

CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. (biolegend.com)

In this work, we isolate a dromedary camel V H H single-domain antibody (also known as nanobody) named D4 and a monoclonal antibody (mAb) named HM2 recognizing membrane-distal and membrane-proximal epitopes of GPC1, respectively. (nature.com)
Our data confirm that VOC, particularly those such as B.1.351 with substitutions at residues 484 and 417, escape neutralization by antibodies directed to the ACE2-binding Class 1 and the adjacent Class 2 epitopes but are susceptible to neutralization by the generally less potent antibodies directed to Class 3 and 4 epitopes on the flanks of the RBD. (nature.com)
The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes. (thermofisher.com)

Objective: To compare serum anti-malondialdehyde-acetaldehyde (anti-MAA) antibody levels and MAA expression in lung tissue from patients with rheumatoid arthritis-associated interstitial lung disease (RA-ILD) to those found in controls. (cdc.gov)
Serum IgA and IgM anti-MAA antibody concentrations were higher in RA-ILD than in RA with COPD or RA alone (P = 0.005). (cdc.gov)
Conclusion: Serum IgA and IgM anti-MAA antibody is associated with ILD among RA patients. (cdc.gov)
At M43, 84%-100% of first-void urine and 98%-100% of serum samples had HPV-specific antibody concentrations. (cdc.gov)
Results show significant Spearman rank correlations between type-specific HPV-antibody concentrations for paired first-void urine and serum at all time points. (cdc.gov)

Moreover, miltuximab, a chimeric anti-GPC1 antibody, was shown to be safe and well tolerated when radiolabeled in a clinical trial 21 . (nature.com)

It has been modified to add detection of antibodies to HPV31,33,45,52 and 58, and to streamline assay and reduce background. (cdc.gov)

Join OmniAb for an exclusive event to celebrate the launch of OmnidAb, the world's first and only transgenic chicken producing human heavy chain only antibodies Event taking place at the Marriott Marquis Rancho Santa Fe Rooms at 7:00 pm. (informaconnect.com)
Find CD200R1 Antibodies for a variety of species such as anti-Human CD200R1, anti-Mouse CD200R1, anti-Rat CD200R1. (antibodies-online.com)

Diagnosis of systemic autoimmune rheumatic diseases (SARD) relies on the presence of hallmark anti-nuclear antibodies (ANA), many of which can be detected years before clinical manifestations. (biomedcentral.com)

We evaluated the duration of neutralizing antibodies and the status of 17DD vaccine-specific T- and B-cell memory following primary and revaccination regimens for yellow fever (YF) in Brazil. (cdc.gov)
Objective To evaluate the efficacy and safety of tabalumab, a monoclonal antibody that neutralises membrane-bound and soluble B-cell activating factor (BAFF), in patients with active rheumatoid arthritis (RA) who showed inadequate response to tumour necrosis factor (TNF) inhibitors. (bmj.com)
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
CD19+ B cell subpopulations were identified on the basis of CD27 and IgD expression. (eur.nl)
Antibodies targeting T-cell inhibitory pathways, such as CTLA-4 and PD-1/PD-L1, are emerging as an important class of cancer therapeutics, and a next generation of immunomodulatory therapies targeting alternative inhibitory (e.g. (bmj.com)
Results show significant expansion of CD4+, CD45R, CD27 and CD62L cell populations in the vaccinated mice group, indicating the high efficacy of the microparticulate vaccine when administered via transdermal route. (cdc.gov)

Anatomy26

Organisms9

Diseases4

Chemicals and Drugs140

Analytical, Diagnostic and Therapeutic Techniques and Equipment43

Phenomena and Processes39

Disciplines and Occupations2

Information Science3

Health Care4

CD707

Antigen5

Member of the TNF-receptor superfamily1

Monoclonal antibodies2

Ligand6

Binds3

Mouse Anti-Human CD271

FITC2

Conjugate1

Clone3

Agonist antibody2

IgG13

Recombinant1

Immunotherapy2

Superfamily3

CD283

Immunoglobulin1

Proliferation4

Soluble CD271

HexaBody-CD273

CD192

Reacts1

Isotype control2

Bispecific antibodies1

Immune2

Proteins5

Thermo Fisher Sci1

Detects2

Responses3

Tumor1

Effector3

KVA121231

Datasheet1

Affinity chromatography3

Cytometric analysis4

Cytotoxicity1

Cells12

Catalog2

ELISA1

Dependent1

Concentration1

S1521

Epitopes3

Serum5

Chimeric1

Assay1

Human2

Clinical1

Cell6