Activation and tumor targeting of iNKT cells via recombinant α-galactosylceramide (αGC)-loaded CD1d-anti-HER2 fusion protein (CD1d-antitumor) is combined or not with OVA peptide/CpG vaccine. (biomedcentral.com)
In addition to TLR ligands, CD1d-restricted invariant NKT (iNKT) cells have been shown to efficiently promote the transactivation of DCs through the CD40L-CD40 interaction upon recognition of the CD1d-glycolipid antigen complex by their semi-invariant TCR. (biomedcentral.com)
The ability to generate CD1d tetramers has provided us with the opportunity of comparing a broad panel of CD1d binding compounds for their ability to stimulate iNKT cells. (dc-research.eu)
CD1d-restricted invariant natural killer T (iNKT) cells actively patrol the liver and possess valuable antitumor potential. (bvsalud.org)
iNKT cells have been traditionally defined by the expression of an invariant TCR α-chain (Vα14-Jα18 in mice or Vα24-Jα18 in humans) and their capacity to recognise the glycolipid antigen α-galactosylceramide (αGalCer) presented on CD1d. (elifesciences.org)
Antigen3
CD1D is the human gene that encodes the protein CD1d, a member of the CD1 (cluster of differentiation 1) family of glycoproteins expressed on the surface of various human antigen-presenting cells. (wikipedia.org)
Fas antigen is a receptor protein that has been linked to many diseases such as cancer1 and. (mblintl.com)
While MHC tetramer staining of antigen specific T cells for flow cytometry is considered highly. (mblintl.com)
Reactivity1
In our hands the vast majority of these TCR β+ NK1.1+ cells are i NKT cells as evidenced by MRS1477 their reactivity with glycolipid-loaded CD1d tetramer (Fig. 5a). (crispr-reagents.com)
Antigens3
They are non-classical MHC proteins, related to the class I MHC proteins, and are involved in the presentation of lipid antigens to T cells. (wikipedia.org)
CD1d-presented lipid antigens activate a special class of T cells, known as natural killer T (NKT) cells, through the interaction with the T-cell receptor present on NKT membranes. (wikipedia.org)
However, except for virus-induced cancers, tumor antigens are mostly self or near-self protein epitopes that are often poorly immunogenic and submitted to central and peripheral tolerance. (biomedcentral.com)
Ligands3
CD1d is also known as R3G1 Some of the known ligands for CD1d are: α-galactosylceramide (α-GalCer), a compound originally derived from the marine sponge Agelas mauritanius with no physiological role but great research utility. (wikipedia.org)
Alternatively, the adjuvant activity of CD1d-restricted invariant NKT cells (iNKT) on the innate and adaptive immunity is well demonstrated, and several CD1d glycolipid ligands are under pre-clinical and clinical evaluation. (biomedcentral.com)
Importantly, additive or even synergistic effects have been shown upon combined CD1d/NKT agonists and TLR ligands. (biomedcentral.com)
Reagents1
Cultures containing i NKT cells that were sorted based on their binding to CD1d tetramer contained high background cytokine levels (data not shown) which is consistent with their partial activation by CD1d tetramer reagents leading to spontaneous cytokine secretion.38 Therefore we stained and isolated hepatic NKT cells based on their concomitant expression of TCR β and NK1.1 and according to standard protocols. (crispr-reagents.com)
Molecules7
CD1d is the only member of the group 2 CD1 molecules. (wikipedia.org)
CD1d tetramers are protein constructs composed of four CD1d molecules joined together and usually fluorescently labelled, used to identify NKT cells or other CD1d-reactive cells. (wikipedia.org)
CD1 molecules that are subsequently to be refolded in vitro in order to generate CD1 tetramers. (dc-research.eu)
The CD1d tetramers were generated from in vitro refolded CD1 molecules. (dc-research.eu)
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). (thermofisher.com)
The basic structure of immunoglobulin (Ig) molecules is a tetramer of two light chains and two heavy chains linked by disulphide bonds. (embl.de)
Ig molecules are highly modular proteins, in which the variable and constant domains have clear, conserved sequence patterns. (embl.de)
Cells4
The combined CD1d/iNKT antitumor therapy and CpG/peptide-based immunization leads to optimized expansion of NK and OVA-specific CD8 T cells (CTLs), likely resulting from the maturation of highly pro-inflammatory DCs as seen by a synergistic increase in serum IL-12. (biomedcentral.com)
We isolated fecal content and small intestinal leukocytes from murine, Roux-en-Y and end-to-end anastomoses, to identify microbiome changes and subsequent alterations in the regulatory and pro-inflammatory immune cells 3 days post-operative. (biomedcentral.com)
Recent technical advances in our laboratory and by other groups allow the direct phenotypic analysis of tetramer-positive cells following ex vivo magnetic enrichment. (nartsignaling.com)
Integrin αvβ6 is expressed exclusively by epithelial cells and functions as a receptor for extracellular matrix proteins such as fibronectin. (mblintl.com)
Therapeutic1
Altogether, our study in mice demonstrates that αGC/CD1d-antitumor fusion protein greatly increases the efficacy of a therapeutic CpG-based cancer vaccine, first as an adjuvant during T cell priming and second, as a therapeutic agent to redirect immune responses to the tumor site. (biomedcentral.com)
Extracellular1
The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. (thermofisher.com)
Vivo1
Two compounds, Compound 1 (1,4-dihydrophenanthrolin-4-one-3-carboxylic acid) and Compound 5 [8-(N-butyl-N-ethylcarbamoyl)-1,4-dihydrophenathrolin-4-one-3-carboxylic acid], with comparable potencies in vivo, were chosen to investigate the effect of the inhibition of the hydroxylation of newly synthesized uterine collagen on the turnover of this protein in vivo. (embl.de)
Monoclonal1
An immunotoxin is a macromolecular drug which consists of a monoclonal antibody linked to a protein toxin.The toxin is usually a cytotoxic protein derived from a bacterial or plant protein, such as Diphtheria toxin (DT), Cholera toxin (CT), Trichosanthin (TCS), Dianthin etc. (creativebiolabs.net)
Epitopes2
Our results demonstrate a diversity of GAD65 responses: at least 12 DR0401-restricted epitopes that can be processed and presented from intact protein. (nartsignaling.com)
As summarized in Table 4, a limited panel of epitopes could detect responses to more than one GAD65 epitope in virtually every subject, allowing visualization and comparison of responses in healthy subjects selleck chemicals llc and in subjects with T1D using tetramers. (nartsignaling.com)
Consistent1
Nevertheless the expression ratios of all these cytokines were significantly greater in cultures receiving a combination of αGC and G7 compared with cultures receiving either treatment alone (Fig. 3b) which is consistent with what was observed at the secreted protein level (Fig. 3a). (crispr-reagents.com)
Intracellular1
Taken with the present results, this suggests that the potential use of inhibitors of prolyl 4-hydroxylase to control excessive collagen deposition in pathological fibrosis may be limited by the need to maintain continuous inhibition of collagen hydroxylation so as to facilitate intracellular degradation of the accumulated protein. (embl.de)
Sequence1
Immunoglobulin-like domains that are related in both sequence and structure can be found in several diverse protein families. (embl.de)
Increasing cell1
This control is propagated through the alpha-subunit of prolyl 4-hydroxylase where protein levels were previously shown to rise fivefold with increasing cell density. (embl.de)
Expression2
Data_Sheet_1.pdf (513K) GUID:?514B8128-1B6A-4BCF-ADFD-8945AF1CB772 Supplementary Table 1: CD1b, CD1d, and CD80 expression* on Mo-DCs from LSD patients. (clinical-research-informatics.com)
Co-expression of Ov-phy-1 with the O. volvulus homologue of protein disulfide isomerase in a baculovirus system resulted in the production of enzymatically active O. volvulus prolyl 4-hydroxylase. (embl.de)
Type1
The KLRB1 protein, NKR-P1A or CD161, is classified as a type II membrane protein because it has an external C terminus. (thermofisher.com)
Class1
Click on the protein counts, or double click on taxonomic names to display all proteins containing IG domain in the selected taxonomic class. (embl.de)
Domain2
Taxonomic distribution of proteins containing IG domain. (embl.de)
The complete taxonomic breakdown of all proteins with IG domain is also avaliable . (embl.de)
Antigen-prese2
CD1D is the human gene that encodes the protein CD1d, a member of the CD1 (cluster of differentiation 1) family of glycoproteins expressed on the surface of various human antigen-presenting cells. (wikipedia.org)
Following encounters with CD1d/αGalCer complexes displayed by antigen-presenting cells (APCs), iNKT cells not only produce a large amount of interferon (IFN)-γ, but also up-regulate surface expression of CD40 ligand (CD40L). (iiarjournals.org)
Molecule3
Using such TCR receptors, iNKT cells recognize lipids and glycolipids, presented by a non-classical MHC molecule known as CD1d ( 10 ). (spandidos-publications.com)
3. The nonclassical class I molecule CD1d associates with the novel CD8 ligand gp180 on intestinal epithelial cells. (nih.gov)
The TCRs of iNKT cells are selected for the recognition of a variety of lipid antigens when bound to a major histocompatibility class I-like molecule, CD1d ( 1 , 2 ). (iiarjournals.org)
INKT4
Activation and tumor targeting of iNKT cells via recombinant α-galactosylceramide (αGC)-loaded CD1d-anti-HER2 fusion protein (CD1d-antitumor) is combined or not with OVA peptide/CpG vaccine. (biomedcentral.com)
The combined CD1d/iNKT antitumor therapy and CpG/peptide-based immunization leads to optimized expansion of NK and OVA-specific CD8 T cells (CTLs), likely resulting from the maturation of highly pro-inflammatory DCs as seen by a synergistic increase in serum IL-12. (biomedcentral.com)
No significant association between the ability of iNKT cells to produce IL‑4 or IFN‑γ and the expression of CD1d on leukemic B lymphocytes or monocytes was identified. (spandidos-publications.com)
In the thymus, CD1d-tetramer+CD24+CD44-NK1.1-CD69+stage 0 iNKT cells express higher levels of Eomes than the other iNKT stages. (bvsalud.org)
Mammalian1
LC3, a mammalian homologue of yeast ATG8, is a soluble protein that is distributed ubiquitously in. (mblintl.com)
Lipid2
We demonstrate that chaperone- and foldase-assisted refolding of denatured CD1d molecules and beta(2)-microglobulin in the presence of synthetic lipids is a rapid method for the generation of functional and specific CD1d tetramers, which unlike previously published protocols ensures isolation of CD1d tetramers loaded with a single lipid species. (ox.ac.uk)
In this study, we report the identification of a membrane-associated protein, Ig-like transcript 4 (ILT4), as a novel human CD1d receptor that inhibits CD1d-mediated immune responses. (ox.ac.uk)
The interaction between ILT4 and CD1d involves the two N-terminal domains of ILT4 and the Ag-binding groove of CD1d (alpha1/alpha2 domain). (ox.ac.uk)
The interaction between ILT4 and CD1d may provide new insights into the regulation of NKT-mediated immunity. (ox.ac.uk)
7. Characterizing CEACAM5 interaction with CD8α and CD1d in intestinal homeostasis. (nih.gov)
18. Binding of divalent H-2Kd/IgG2aFc fusion protein to murine macrophage via Fc-FcR interaction. (nih.gov)
Human2
Human CD1d-glycolipid tetramers generated by in vitro oxidative refolding chromatography. (ox.ac.uk)
We here report the construction of CD1d-glycolipid tetramers from fully denatured human CD1d molecules by using the technique of oxidative refolding chromatography. (ox.ac.uk)
Specific1
13. A11 Tetramer-assisted characterization of Rta-specific CD8+ T-cell responses in healthy virus carriers. (nih.gov)
Binding1
The agent most efficient in binding to CD1d is a synthetic compound that originated from marine sponges, known as α-galactosylceramide (α-GalCer). (spandidos-publications.com)
Leads1
This damage leads to extravasation of blood-borne proteins, formation of brain edema, and neuronal damage. (tgf-betasignal.com)