• IO-108 is a fully human IgG4 monoclonal antibody with high affinity and specificity towards LILRB2 (also known as ILT4). (financialcontent.com)
  • The specificity of this antibody has been confirmed at the First Human TcR Monoclonal Antibody Workshop in San Francisco in 1995. (beckman.com)
  • Specificity : The mouse monoclonal antibody JJ319 reacts with an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells. (abeomics.com)
  • Likewise, antigen/antibody complexes are bound by the FcR of APCs and processed in a unique fashion dependent on the epitope specificity of the bound antibody. (hindawi.com)
  • The epitope specificity of the antibodies in relapsing polychondritis differs from those in RA, suggesting different mechanisms for formation and pathophysiologic roles. (medscape.com)
  • Rat anti Mouse CD1d antibody, clone 1B1 ( MCA4700 ) used for immunofluorescence staining of mouse tissues. (bio-rad-antibodies.com)
  • Many hematopoietic cell types express CD1d and so are with the capacity of presenting glycolipid antigens to invariant natural killer T?cells (iNKT cells). (biotech-angels.com)
  • Graphical Abstract Introduction Natural killer T?cells with invariant T?cell receptor α chains (iNKT cells) are a conserved population that recognizes KM 11060 glycolipid antigens bound to CD1d a lipid antigen-presenting molecule with structural similarities to major histocompatibility complex (MHC) class I proteins (Brennan et?al. (biotech-angels.com)
  • ANA production can precede symptoms and indications of disease by 5 or more years, with more complete serological evaluation demonstrating increasing creation of antibodies to nuclear antigens such as for example DNA, Sm, RNP, Ro, and La.4 This stage of disease could be known as preautoimmunity because symptomatology isn't manifest. (columbiagypsy.net)
  • The evidence for an autoimmune etiology includes pathological findings of infiltrating T cells, the presence of antigen-antibody complexes in affected cartilage, cellular and humoral responses against collagen type II and other collagen antigens, and the observation that immunosuppressive regimens most often suppress the disease. (medscape.com)
  • No. 553988, dashed line histogram) or with the FITC Rat Anti-Mouse CD1d antibody (Cat. (bdbiosciences.com)
  • The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. (bdbiosciences.com)
  • C57BL/6 mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, Super Bright 600 (Product # 63-4031-82) (left) or 0.25 µg of CD3 Monoclonal Antibody, Super Bright 600 (right). (thermofisher.com)
  • A. Representative spleen sections from 3 mo old B6.TC (left) and B6 (right) mice stained with Moma-1-FITC, CD1d-PE and B220-PB. (bio-rad-antibodies.com)
  • Representative spleen sections from 10 mo old B6.TC (left) and B6 (right) mice stained with CD4-FITC, CD1d-PE and B220-PB. (bio-rad-antibodies.com)
  • Find CD200R1 Antibodies with a specific conjugate such as PE, FITC, APC. (antibodies-online.com)
  • Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. (thermofisher.com)
  • iNKT cells have been traditionally defined by the expression of an invariant TCR α-chain (Vα14-Jα18 in mice or Vα24-Jα18 in humans) and their capacity to recognise the glycolipid antigen α-galactosylceramide (αGalCer) presented on CD1d. (elifesciences.org)
  • By visualizing glycolipid antigen presentation directly with monoclonal antibodies specific for complexes of αGC bound to CD1d we showed that the CD8α+DEC-205+ subset of dendritic cells was the major APC in the spleen for a range of αGC analogs irrespective of their chemical structures and cytokine biasing activities. (biotech-angels.com)
  • Invariant natural killer T (iNKT) cells are CD1d-restricted T cells, which respond rapidly to antigen recognition and promote development of anti-tumor immunity in many tumor models. (biomedcentral.com)
  • α-Galactosylceramide (α-GalCer) can be presented by CD1d molecules of antigen-presenting cells, and is known to induce a potent NKT cell-dependent cytotoxic response against tumor cells. (biomedcentral.com)
  • Independent of antibody secretion B cells secrete proinflammatory cytokines, support the formation of ectopic GCs, and serve as antigen presenting cells. (hindawi.com)
  • c) Effect of antibodies and antigen-specific B cells on antigen uptake. (hindawi.com)
  • Left panel: antigen bound by antibody is taken up via FcR on APCs such as dendritic cells or macrophages. (hindawi.com)
  • 2012 However other studies yielded different conclusions identifying pharmacokinetic properties of the glycolipids or localization of CD1d molecules containing bound glycolipids to different membrane microdomains as major determinants of cytokine skewing in the response to iNKT cell activation (Im et?al. (biotech-angels.com)
  • Surprisingly, we previously found that mice deficient in iNKT cells developed spontaneous CD8 + T cells responses partially effective at inhibiting metastases in mice bearing the 4T1 mammary carcinoma, and showed a markedly improved response to treatment with local radiotherapy and anti-CTLA-4 antibody compared to wild type (WT) mice. (biomedcentral.com)
  • To determine if the improved response to treatment in the absence of iNKT cells was independent from the immunotherapy employed, 4T1-tumor bearing WT and iNKT −/− mice were treated with local radiotherapy in combination with antibody-mediated CD137 co-stimulation. (biomedcentral.com)
  • The mouse monoclonal antibody 6B11 recognizes an extracellular epitope within V alpha 24 J alpha 18 TCR, which is expressed by invariant NK T cells (iNKT). (exbio.cz)
  • Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. (thermofisher.com)
  • In addition, preclinical data for IO-312, a novel bispecific antibody targeting LILRB4 (ILT3) and CD3 (LILRB4 x CD3) will be presented as a late-breaking poster at AACR 2023. (financialcontent.com)
  • Preclinical studies have shown that this antibody targets IL12 to regions of tumor necrosis by binding histones on free DNA fragments in these areas, resulting in enhanced antitumor activity. (aacrjournals.org)
  • See more details about Hi-Affi™ recombinant antibody benefits . (creativebiolabs.net)
  • Download resources about recombinant antibody development and antibody engineering to boost your research. (creativebiolabs.net)
  • Furthermore, levels of antibodies to matrilin 1, an extracellular matrix protein predominantly expressed in tracheal cartilage, were significantly higher in patients with relapsing polychondritis, especially in those with respiratory symptoms, than in patients with Wegener granulomatosis , systemic lupus erythematosus , or RA and in healthy controls. (medscape.com)
  • An isotype control should be used at the same concentration as the antibody of interest. (bdbiosciences.com)
  • The effects of antibody-mediated blockade of CD1d on DC number and phenotype, priming of anti-tumor T cells, and tumor response to treatment with local radiotherapy and anti-CTLA-4 antibody were evaluated. (biomedcentral.com)
  • This product is a mouse monoclonal antibody that can be potentially used in the treatment of hematopoietic tumor. (creativebiolabs.net)
  • The Fc portion of antibodies in immune complexes can be bound by C1q of the classical complement pathway, which eventually leads to the release of C5a and C3a. (hindawi.com)
  • The following product was used in this experiment: NK1.1 Monoclonal Antibody (PK136), Functional Grade, eBioscience™ from Thermo Fisher Scientific, catalog # 16-5941-82, RRID AB_469159. (thermofisher.com)
  • The following product was used in this experiment: CD3 Monoclonal Antibody (17A2), Super Bright™ 600, eBioscience™ from Thermo Fisher Scientific, catalog # 63-0032-82, RRID AB_2802423. (thermofisher.com)
  • Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. (thermofisher.com)
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. (thermofisher.com)
  • The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
  • The antibody was purified by affinity chromatography. (biolegend.com)
  • Flow cytometric analysis of CD1d expression on mouse thymocytes. (bdbiosciences.com)
  • Applications Tested: The PK136 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes and can be used at less than or equal to 0.5 µg per test. (thermofisher.com)
  • Applications Reported: This 17A2 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
  • Applications Tested: This 17A2 antibody has been tested by flow cytometric analysis of mouse splenocytes. (thermofisher.com)
  • 2011 In contrast glycolipids that induce responses that are biased toward Th1 cell cytokines are more hydrophobic and require intracellular loading onto CD1d for presentation (Arora et?al. (biotech-angels.com)
  • CD1d blockade restored the number of DC in WT mice, improved T cell priming in draining lymph nodes and significantly enhanced response to treatment. (biomedcentral.com)
  • PD-L1 and PD-1 blockade using antibodies are in human clinical trials as an alternative cancer treatment modality. (studylib.net)
  • Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.cn)
  • 2009 Because most cells of hematopoietic origin express CD1d (Brossay et?al. (biotech-angels.com)
  • This unique lymphocyte population restricted by the CD1d molecule recognition has been identified as the natural killer T (NKT) cells. (beckman.com)
  • The 1B1 antibody competes with mAb 3C11 in binding to mouse splenocytes. (bdbiosciences.com)
  • MAb 1B1 detects CD1d at varying levels on most types of bone marrow and peripheral leukocytes and on epithelial, dendritic, and lymphoid cells in the thymus. (bdbiosciences.com)
  • However, CD1d expression was not detectable via flow cytometry on intestinal epithelial cells in studies using the anti-CD1d mAbs 3C11, 1B1, and 9C7. (bdbiosciences.com)
  • For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. (thermofisher.com)
  • it's not expressed in CD1d-restricted NKT cells. (biolegend.com)
  • In these studies, CD4 + T cell-depleting and blocking antibodies caused remission from disease in a number of CD and UC patients examined, suggesting a prominent role of CD4 + T cells in propagating disease ( 1 , 2 ). (frontiersin.org)
  • Although initially identified by co-expression of conventional αβ T-cell receptors (TCR) and markers typically associated with natural killer (NK) cells [ 3 ], NKT are currently distinguished on the basis of CD1d restriction as well as specific usage of TCRα chains [ 4 ]. (biomedcentral.com)
  • Through these mechanisms B cells are involved both in autoimmune diseases that are traditionally viewed as antibody mediated and also in autoimmune diseases that are commonly classified as T cell mediated. (hindawi.com)
  • B cells have antibody-dependent and antibody-independent pathogenic functions. (hindawi.com)
  • Binding of the Fc portion of the antibodies to FcR leads to activation of effector cells and further release of proinflammatory cytokines and proteolytic enzymes, mediators of antibody-dependent cell-mediated cytotoxicity (ADCC). (hindawi.com)
  • CD1d-dependent expansion of NKT follicular helper cells in vivo and in vitro is a product of cellular proliferation and differentiation. (ouhsc.edu)
  • MBLI is proud to introduce the Anti-Integrin αvβ6 ELISA Kit for measurement of anti-integrin αvβ6 antibody levels in human serum. (mblintl.com)
  • It blocks the interaction of LILRB2 with multiple ligands that are involved in cancer-associated immune suppression, including HLA-G, ANGPTLs, SEMA4A and CD1d. (financialcontent.com)
  • It is a U.S. Food and Drug Administration (FDA) approved human monoclonal antibody, and works by activating the immune system by targeting CTLA-4. (creativebiolabs.net)
  • Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR Valpha24-Jalpha18 (6B11) purified antibody (concentration in sample 0.56 μg/ml, GAM APC). (exbio.cz)
  • Separation of human CD3 positive TCR Valpha24-Jalpha18 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR Valpha24-Jalpha18 (6B11) purified antibody (concentration in sample 0.56 μg/ml, GAM APC). (exbio.cz)
  • Find CD200R1 Antibodies for a variety of species such as anti-Human CD200R1, anti-Mouse CD200R1, anti-Rat CD200R1. (antibodies-online.com)
  • D, representative immunoblots of antibody responses in 4 bevacizumab plus ipilimumab-treated patients to a total of zero, one, two, or three galectins. (creativebiolabs.net)
  • Researchers have found that antibodies to type II collagen are present during acute relapsing polychondritis episodes and that the levels correlate with the severity of the episode. (medscape.com)
  • They are more likely to be found in association with antibodies to type II collagen in patients with relapsing polychondritis. (medscape.com)
  • 2011 A unifying feature of αGC analogs that induce predominantly Th2 cell-associated cytokines is that they are more polar than KRN7000 and can load directly onto CD1d molecules on the cell surface (Im et?al. (biotech-angels.com)
  • When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. (thermofisher.com)
  • Most patients with relapsing polychondritis had high titers of antifetal cartilage antibodies during the early acute phase. (medscape.com)
  • Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins. (abcam.cn)
  • Various studies find circulating antibodies to cartilage-specific collagen types II, IX, and XI to be present in 30%-70% of patients with relapsing polychondritis. (medscape.com)
  • it has been proposed that antibodies are formed as a primary event in relapsing polychondritis. (medscape.com)