• Differentially expressed genes of CD11b+Gr-1+ immature myeloid cells (IMCs) in the bone marrow and colonic tumor setting of histidine decarboxylase (HDC)-KO mice were examined by microarray (Affymetrix Mouse 430.2 array). (gsea-msigdb.org)
  • Several studies have shown that adipocytes highly express CD1d, which presents lipid antigens to invariant natural killer T (iNKT) cells and stimulates the activation of iNKT cells ( 5 - 7 ). (frontiersin.org)
  • Rat anti Mouse CD1d antibody, clone 1B1 ( MCA4700 ) used for immunofluorescence staining of mouse tissues. (bio-rad-antibodies.com)
  • Early therapeutic antibody was usually produced in murine host, leading to serious human anti-mouse antibody expression called HAMA reaction, which in turn interfered with the therapeutic effects. (creativebiolabs.net)
  • In 1975, Kohler and Milstein introduced an in vitro cultured hybridoma secreting anti-sheep red blood cell monoclonal antibody by fusing mouse myeloma cell and mouse spleen cells. (creativebiolabs.net)
  • In 1984, Morrison firstly introduced chimeric antibody using recombinant DNA technology, joining mouse antibody variable region gene to human antibody constant region gene. (creativebiolabs.net)
  • Combining the mouse variable region with human constant region improved the monoclonal antibody humanization proportion to 70%, which reduced HAMA. (creativebiolabs.net)
  • In the brain of the amyloid precursor protein transgenic mice, deletion of ABCA7 resulted in accumulation of Aβ40 and Aβ42 in the early stage apparently by accelerating Aβ production. (wikipedia.org)
  • In vitro and in vivo experiments showed that iNKT cells promote differentiation of monocytes to M2 macrophages in an IL-4 and CD1d-dependent process. (ox.ac.uk)
  • In today's study, we looked into the immunological function of Kupffer cells/macrophages in CCl4-induced hepatitis in mice. (enmd-2076.com)
  • The populace of Compact disc11b+ Kupffer cells/macrophages significantly elevated 24 hour (h) after CCl4 administration, in c-lipo-pretreated mice especially. (enmd-2076.com)
  • Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.cn)
  • We demonstrate here that the absence of invariant NKT (iNKT) cells in mice during IAV infection resulted in the expansion of myeloid-derived suppressor cells (MDSCs), which suppressed IAV-specific immune responses through the expression of both arginase and NOS, resulting in high IAV titer and increased mortality. (ox.ac.uk)
  • The crosstalk between iNKT and MDSCs was CD1d- and CD40-dependent. (ox.ac.uk)
  • In contrast, iNKT-deficient mice showed higher numbers of Ly6C(hi) monocytes, reduced M2, and much more severe disease. (ox.ac.uk)
  • Adoptive transfer of M2-enriched cells to iNKT-deficient mice markedly improved neurologic impairment. (ox.ac.uk)
  • All four adenosine receptors are expressed on the surface of mouse invariant NKT (iNKT) cells. (guidetomalariapharmacology.org)
  • More rapid endocytosis of amyloid precursor protein was observed in primary microglia from ABCA7 deficient mice. (wikipedia.org)
  • RESULTS: PDC-deficient mice showed exacerbation of inflammatory and arthritis symptoms after arthritogenic serum transfer. (cnrs.fr)
  • Control, NK cell-deficient, and NKT cell-deficient mice underwent CLP. (aai.org)
  • Results show that mice treated with anti-asialoGM1 (NK cell deficient) or anti-NK1.1 (NK/NKT cell deficient) show less systemic inflammation and have improved survival compared with IgG-treated controls. (aai.org)
  • CD1 knockout mice (NKT cell deficient) did not demonstrate decreased cytokine production or improved survival compared with wild type mice. (aai.org)
  • Invariant NKT cells reduce the immunosuppressive activity of influenza A virus-induced myeloid-derived suppressor cells in mice and humans. (ox.ac.uk)
  • In addition to mechanisms of microbe-host interaction, the role of cell stress related to unfolded protein responses (UPR) of the endoplasmic reticulum (ER) and mitochondria is characterized in the context of inflammatory and tumorigenic processes using novel tissue-specific mouse models. (intestinal-microbiota.de)
  • Yersinia enterocolitica targets cells of the innate and adaptive immune system by injection of Yops in a mouse infection model. (intestinal-microbiota.de)
  • While AND-34 is widely expressed in wild type mice, histologic analysis of multiple organs in AND-34 −/− mice is unremarkable and analyses of lymphocyte development show no overt changes. (molvis.org)
  • CXCR1 expression to improve anti-cancer efficacy of intravenously injected CAR-NK cells in mice w. (cytomed.sg)
  • The constructed chimeric vector was transfected into mouse myeloma cells and the cells expressed chimeric antibodies. (creativebiolabs.net)
  • A. Representative spleen sections from 3 mo old B6.TC (left) and B6 (right) mice stained with Moma-1-FITC, CD1d-PE and B220-PB. (bio-rad-antibodies.com)
  • Representative spleen sections from 10 mo old B6.TC (left) and B6 (right) mice stained with CD4-FITC, CD1d-PE and B220-PB. (bio-rad-antibodies.com)
  • Enhancing immunostimulatory function of human embryonic stem cell-derived dendritic cells by CD1d. (cytomed.sg)
  • It really is today generally accepted which the livers of mice and human beings contain types of innate immune system cells [11]C[13]. (enmd-2076.com)
  • METHODS: Mice with K/BxN serum-induced arthritis, collagen-induced arthritis, and human tumor necrosis factor transgene insertion were studied. (cnrs.fr)
  • In contrast, enhancing PDC recruitment and activation to arthritic joints by topical application of the Toll-like receptor 7 (TLR-7) agonist imiquimod significantly ameliorated arthritis in various mouse models. (cnrs.fr)
  • Analyses of in vitro cell function, apoptosis, the cell cycle and cancer stemness were performed using GC cell lines with a stable knockout of a candidate gene. (nagoya-u.ac.jp)
  • Gnotobiotic mouse models and metagenomic analyses will provide new insights into the intricate interaction between Yersinia , the intestinal microbiota and the mucosal immune system, and might result in novel strategies for treatment of enteric infections. (intestinal-microbiota.de)
  • Mechanisms of microbe-host interactions are specified by the use of germ-free mouse models largely focusing on inflammatory bowel diseases (IBD). (intestinal-microbiota.de)
  • A small percentage of AND-34 −/− mice show distinctive small white eye lesions resulting from the migration of ruptured cortical lens tissue into the anterior chamber. (molvis.org)
  • Lack of ABCA7 gene exhibits phenotypes in mice, such as moderate decrease in plasma HDL and adipose tissues only in female, while release of cellular cholesterol and phospholipid is not impaired. (wikipedia.org)
  • [ 90 ] In nonobese diabetic (NOD) mice that are classically used for diabetes studies, iNKT cells exhibit remarkable defects in both their frequency and cytokine production. (medscape.com)
  • [ 90 ] Adoptive transfer of iNKT cells from wild-type mice or, alternatively, the restoration of their function by αGalCer treatment or TCR or CD1d overexpression can prevent the development of diabetes in NOD mice. (medscape.com)
  • Here, we show the impact of Eomes on iNKT cells in the thymus and peripheral tissue using conditional knockout (Eomes-cKO) mice. (bvsalud.org)
  • In the thymus, CD1d-tetramer+CD24+CD44-NK1.1-CD69+stage 0 iNKT cells express higher levels of Eomes than the other iNKT stages. (bvsalud.org)
  • In addition, vaccination of C57BL/6, but not Eomes-cKO mice with iNKT ligand-loaded dendritic cells generated KLRG1+iNKT cells in lung, characterized as effector memory phenotype by transcriptome profiling. (bvsalud.org)
  • Although autophagy modulates MHC antigen presentation, it remains unclear whether autophagy defects impact on CD1d lipid loading and presentation to invariant natural killer T (iNKT) cells and on iNKT cell differentiation in the thymus. (ox.ac.uk)
  • We report that, in mice with a conditional deletion of the essential autophagy gene Atg7 in the T-cell compartment (CD4 Cre-Atg7(-/-)), thymic iNKT cell development--unlike conventional T-cell development--is blocked at an early stage and mature iNKT cells are absent in peripheral lymphoid organs. (ox.ac.uk)
  • In vitro and in vivo experiments showed that iNKT cells promote differentiation of monocytes to M2 macrophages in an IL-4 and CD1d-dependent process. (ox.ac.uk)
  • First, NKT cells can impair the differentiation of anti-islet reactive T cells into Th1 effector cells in a cell-cell contact dependent manner, which did not require Th2 cytokine production or CD1d recognition (Figure 2A). (medscape.com)
  • Mice pretreated with TCDD developed exacerbated liver injury in response to administration of a mild dose (6 mg/kg) of Con A. In the present study, we tested the hypothesis that TCDD pretreatment exacerbates Con A-induced liver injury by enhancing the activation and recruitment of accessory cell types including neutrophils, macrophages, and natural killer (NK) cells. (nih.gov)
  • NKT cell-deficient (CD1d KO) mice were used to examine whether NKT cells were required for TCDD/Con A-induced liver injury. (nih.gov)
  • The use of human CD1d-alpha-galactosylceramide tetramers for ex vivo staining of peripheral blood lymphocytes and intrahepatic T cells from patients with viral liver cirrhosis allowed for the first time simultaneous analysis of frequency and specificity of natural killer T cells in human clinical samples. (ox.ac.uk)
  • Reduction of CD3 or CD1d may lead to dysfunction of T cells by deletion of ABCA7. (wikipedia.org)
  • Severely immunodeficient mice engrafted with functional human cells and tissues, known as 'humanized' mice, facilitate progress in studies of human haematopoiesis, immunity, gene therapy, infectious diseases, cancer and regenerative medicine. (nature.com)
  • Mice homozygous for the severe combined immunodeficiency ( scid ) gene mutation or for targeted mutations at the recombination-activating gene 1 ( Rag1 ) or Rag2 loci, that also have a targeted mutation at the interleukin-2 receptor γ-chain ( Il2rg ) locus, support high levels of engraftment and function of human haematopoietic stem cells (HSCs) and human immune systems. (nature.com)
  • The development of humanized mice with functional human immune systems (generated by the engraftment of human lymphoid tissues, HSCs or peripheral blood mononuclear cells) provides an opportunity to carry out translational research on human immunity and autoimmune diseases, and for the study of the biology of the human pathogens responsible for AIDS and several other human-specific infectious diseases. (nature.com)
  • The phenotypical and functional characterization of human tumour stem cells is also being advanced through the study of humanized mice. (nature.com)
  • Significant advances in our understanding of the in vivo functions of human cells and tissues and the human immune system have resulted from the development of 'humanized' mouse strains that are based on severely immunodeficient mice with mutations in the interleukin-2 receptor common γ-chain locus. (nature.com)
  • 7. CXCR6 deficiency ameliorates ischemia-reperfusion injury by reducing the recruitment and cytokine production of hepatic NKT cells in a mouse model of non-alcoholic fatty liver disease. (nih.gov)
  • 12. Critical role for the chemokine receptor CXCR6 in homeostasis and activation of CD1d-restricted NKT cells. (nih.gov)
  • Adoptive transfer of M2-enriched cells to iNKT-deficient mice markedly improved neurologic impairment. (ox.ac.uk)
  • However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen. (thermofisher.com)
  • Notably, Pim1 over-expression failed to promote development and survival of any T-lineage cells other than αβ T cells, as we observed complete lack of γδ, NKT, FoxP3(+) T regulatory cells and TCR-β(+) CD8αα IELs in Pim1(Tg) γc(KO) mice. (nih.gov)
  • Human and mouse gut-resident CD8 + T cells have reduced mitochondrial mass, but maintain a viable energy balance to sustain their function. (biomed.news)
  • All experiments performed on mouse blood gated on live single cells, in the presence of 10% mouse serum. (bio-rad-antibodies.com)
  • Rat anti Mouse CD45R antibody, clone RA3-6B2 ( MCA1258 ) used for the enumeration of B cells in the intestinal mucosa by immunofluorescence. (bio-rad-antibodies.com)
  • We show that autophagy increases during iNKT cell thymic differentiation and that it developmentally regulates mitochondrial content through mitophagy in the thymus of mice and humans. (ox.ac.uk)
  • Einige unserer Partnerfirmen validieren ihre Antikörper darüber hinaus besonders extensiv, mittels knockout/knockdown des Zielgens, durch verschiedene Techniken und mit unterschiedlichen Gewebetypen, um besonders hohen Qualitätsstandards zu entsprechen. (biozol.de)
  • 5. NADPH Oxidase 1 in Liver Macrophages Promotes Inflammation and Tumor Development in Mice. (nih.gov)
  • Genes down-regulated in IL10 [GeneID=3586] knockout macrophages: unstimulated versus LPS. (gsea-msigdb.org)
  • Bone marrow-derived macrophages were produced from mice lacking IL-10 alone (IL10-def) or mice lacking both IL-10 and the p50/p105 subunit of NF-kB (p50/IL10), and left unstimulated, stimulated with LPS (1 ng/ml) or stimulated with LPS and IL-10 (0.3 ng/ml). (gsea-msigdb.org)
  • Obese AT is characterized by increased numbers and (especially in mice) proportions of macrophages. (comprehensivephysiology.com)
  • More rapid endocytosis of amyloid precursor protein was observed in primary microglia from ABCA7 deficient mice. (wikipedia.org)
  • 11. Loss of immunity-supported senescence enhances susceptibility to hepatocellular carcinogenesis and progression in Toll-like receptor 2-deficient mice. (nih.gov)
  • In contrast, iNKT-deficient mice showed higher numbers of Ly6C(hi) monocytes, reduced M2, and much more severe disease. (ox.ac.uk)
  • Consequently, we asked whether Pim1 overexpression would suffice to restore lymphocyte development in γc-deficient mice. (nih.gov)
  • By analyzing Pim1-transgenic γc-deficient mice (Pim1(Tg) γc(KO) ), we show that Pim1 promoted T-cell development and survival in the absence of γc. (nih.gov)
  • Lack of ABCA7 gene exhibits phenotypes in mice, such as moderate decrease in plasma HDL and adipose tissues only in female, while release of cellular cholesterol and phospholipid is not impaired. (wikipedia.org)
  • We here report the construction of CD1d-glycolipid tetramers from fully denatured human CD1d molecules by using the technique of oxidative refolding chromatography. (ox.ac.uk)
  • We demonstrate that chaperone- and foldase-assisted refolding of denatured CD1d molecules and beta(2)-microglobulin in the presence of synthetic lipids is a rapid method for the generation of functional and specific CD1d tetramers, which unlike previously published protocols ensures isolation of CD1d tetramers loaded with a single lipid species. (ox.ac.uk)
  • In addition, humanized mouse models have benefited greatly from the identification of human species-specific molecules that are crucial for the engraftment and function of human haematopoietic and immune systems and the expression of these molecules in the immunodeficient recipient. (nature.com)
  • Natural resistance to infection with mouse cytomegalovirus (MCMV) is controlled by a dominant locus, Cmv1 . (silverchair.com)
  • 4. Roles of CCR2 and CCR5 for Hepatic Macrophage Polarization in Mice With Liver Parenchymal Cell-Specific NEMO Deletion. (nih.gov)
  • TLRs, Rig-I, Dectin-1) within APCs, with the consequential induction of CD1d-mediated Ag presentation and release of proinflammatory cytokines. (nih.gov)
  • In this Review, we discuss recent advances in the development and utilization of humanized mice, the lessons learnt, the remaining challenges and the promise of using humanized mice for the in vivo study of human immunology. (nature.com)
  • Development and molecular characterization of new mice models for human diseases. (jax.org)
  • My research is focused in the development and characterization of new mice models for human diseases. (jax.org)
  • Thus, we are contributing to the research community with better mice models and setting new platforms for future development of more precise therapeutic strategies. (jax.org)
  • Additional genetic and technological modifications continue to accelerate progress towards the development of a robust functional human immune system in humanized mice. (nature.com)
  • These mouse strains support the engraftment of a functional human immune system and permit detailed analyses of human immune biology, development and functions. (nature.com)
  • 14. An Immune Gene Expression Signature Associated With Development of Human Hepatocellular Carcinoma Identifies Mice That Respond to Chemopreventive Agents. (nih.gov)
  • The RA3-6B2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low and high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. (thermofisher.com)
  • The RA3-6B2 antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 5 µg/mL. (thermofisher.com)
  • Human CD1d-glycolipid tetramers generated by in vitro oxidative refolding chromatography. (ox.ac.uk)
  • The set represents ~36,000 full length genes and EST clusters derived from sequence clusters in Build 74 of the Mouse Unigene Database. (nih.gov)
  • Together with the generation of transgenic mouse models expressing human gene variants, we are expanding the genetic tools available for the scientific community in areas like ALS, Charcot-Marie-Tooth, lysosomal storage diseases and several others rare disorders. (jax.org)
  • Figure B. FITC conjugated Rat anti Mouse CD19 ( MCA1439F ) and Amethyst Orange conjugated Rat anti Mouse CD45R ( MCA1258AMO ). (bio-rad-antibodies.com)
  • We are applying cutting-edge CRISPR/Cas9-based genome editing technology for a precise engineering of the mouse genome with the goal of reproduce human clinical alleles (knock in and knock out models). (jax.org)
  • Humanized mice are being used as hosts for primary human tumours for studies of tumour growth and metastasis and for experimental cancer therapy. (nature.com)
  • The potential for new advances in our understanding of human immunology and other areas of human biology that are supported by studies in humanized mice remains promising. (nature.com)
  • Figure 1: Approaches for the engraftment of human immune systems in immunodeficient mice. (nature.com)
  • Figure A. FITC conjugated Rat anti Mouse CD19 ( MCA1439F ) and Amethyst Orange conjugated Rat IgG2a isotype control ( MCA6005AMO ). (bio-rad-antibodies.com)
  • CD1d KO mice were completely protected from liver injury induced by treatment with Con A alone, whereas the injury from TCDD/Con A treatment was reduced but not eliminated. (nih.gov)
  • However, T-cell deficient (RAG1 KO) mice were protected from liver injury induced by Con A irrespective of pretreatment with TCDD. (nih.gov)
  • 6. Death receptor-independent FADD signalling triggers hepatitis and hepatocellular carcinoma in mice with liver parenchymal cell-specific NEMO knockout. (nih.gov)
  • Advances in humanized mice over the past few years have included approaches to decrease host innate immune responses. (nature.com)
  • By using the knock-out mice, ABCA7 was indicated involved in generation and processing of amyloid β (Aβ) peptides. (wikipedia.org)
  • Utilizing mice models as a platform for pre-clinical evaluation of new therapies. (jax.org)