• Cationic liposomes are spherical structures that contain positively charged lipids. (wikipedia.org)
  • In 1987, Philip Felgner published the first approach to using cationic lipids to transfect DNA into cells, based on his research into cationic lipids at Syntex from 1982 to 1988. (wikipedia.org)
  • The use of cationic lipids helps to enhance the overall stability and efficiency of liposomes. (wikipedia.org)
  • Although cationic lipids themselves are able to encapsulate nucleic acids into liposomes, the transfection efficiency is low due to a process known as endosomal escape. (wikipedia.org)
  • The addition of helper lipids to the cationic lipids demonstrates a much higher transfection efficiency. (wikipedia.org)
  • Cationic liposomes with a higher transfection efficiency are composed of a cationic phospholipid, and a few neutral helper lipids. (wikipedia.org)
  • Compared with conventional CL-DNA preparation techniques involving hand-shaking or vortexing, the PMRI system demonstrates a greater ability to constantly and uniformly mix cationic lipids and DNA simultaneously. (rsc.org)
  • There are three types of lipids, anionic (negatively charged), neutral, or cationic (positively charged). (genetherapynet.com)
  • The cationic lipids also protect against degradation of the DNA by the cell. (genetherapynet.com)
  • Reported here is the correlation between the transfection efficiency of cationic liposome/DNA complexes (lipoplexes) and the structural evolution that they undergo when interacting with anionic membrane lipids. (unicam.it)
  • Most importantly, structural changes induced by anionic lipids strictly depended on the lipid composition of lipoplexes. (unicam.it)
  • Here we advance the concept that structural stability, upon interaction with cellular anionic lipids, is a key factor governing the transfection efficiency of lipoplexes. (unicam.it)
  • These nanoparticles in combination with cationic lipids and polymers form complexes, lipoplexes and polyplexes, with nucleic acids to be inserted (siRNA, plasmid DNA, or proteins). (sirnatransfection.org)
  • Nonviral gene therapy utilizing plasmid DNA (pDNA) complexed with cationic lipids (lipoplexes) or cationic polymers (polyplexes) has demonstrated considerable potential for the treatment of a variety of diseases. (ox.ac.uk)
  • Preparation of lipoplexes for in vivo therapy The cationic liposome DOTAP and cholesterol were purchased from Avanti Polar Lipids (Alabaster, AL, USA) and Sigma (St. Louis, MO, USA), respectively. (hormonessignaling.com)
  • Design of the cationic ionizable lipids, which are a key component of the LNP formulations, with an acid dissociation constant (pKa) close to the early endosomal pH, would not only ensure effective encapsulation of mRNA into the stabilized lipoplexes within the LNPs, but also its subsequent endosomal release into the cytoplasm after endocytosis. (awljournal.org)
  • The thesis entitled "The Role of Liposomal Hybrids and Gold Nanoparticles in the Efficacious Transport of Nucleic Acids and Small Molecular Drugs for Cancer Nanomedicine" elucidates the preparation of various liposomal formulations of cationic monomeric and gemini lipids where hydrophobic domains were consisted of tocopherol, cholesterol and pseudoglyceryl backbone for the cellular transport of nucleic acids. (iisc.ac.in)
  • Mechanistic Insights into Lipoplex Internalization and Therapeutic Induction of Apoptotic Activity In this chapter, we present cationic dimeric (gemini) lipids for significant plasmid DNA (pDNA) delivery to different cell lines without any marked toxicity in the presence of serum. (iisc.ac.in)
  • Chapter 1B: Efficacious Gene Silencing in Serum and Significant Apoptotic Activity Induction by Survivin Downregulation Mediated by Cationic Gemini Tocopheryl Lipids Non-viral gene delivery offers cationic liposomes as promising instruments for the delivery of double-stranded RNA (ds RNA) molecules for successful sequence-specific gene silencing (RNA interference). (iisc.ac.in)
  • We discuss in this chapter about six tocopherol based cationic gemini lipids, which induce substantial gene knockdown without any obvious cytotoxicity. (iisc.ac.in)
  • These cationic lipids may be incorporated into a lipid particle for delivering an active agent, such as a nucleic acid. (justia.com)
  • Cationic liposomes can vary in size between 40 nm and 500 nm, and they can either have one lipid bilayer (monolamellar) or multiple lipid bilayers (multilamellar). (wikipedia.org)
  • The positive charge of the phospholipids allows cationic liposomes to form complexes with negatively charged nucleic acids (DNA, mRNA, and siRNA) through ionic interactions. (wikipedia.org)
  • Upon interacting with nucleic acids, cationic liposomes form clusters of aggregated vesicles. (wikipedia.org)
  • These interactions allow cationic liposomes to condense and encapsulate various therapeutic and diagnostic agents in their aqueous compartment or in their lipid bilayer. (wikipedia.org)
  • Due to the overall positive charge of cationic liposomes, they interact with negatively charged cell membranes more readily than classic liposomes. (wikipedia.org)
  • These issues can be reduced by optimizing the physical and chemical properties of cationic liposomes through their lipid composition. (wikipedia.org)
  • Cationic liposomes are increasingly being researched for use as delivery vectors in gene therapy due to their capability to efficiently transfect cells. (wikipedia.org)
  • A common application for cationic liposomes is cancer drug delivery. (wikipedia.org)
  • These components are all biocompatible and biodegradable in the human body, making cationic liposomes a useful gene delivery vector. (wikipedia.org)
  • The presence of PEG on the surface of the liposome drastically increases the blood circulation time of cationic liposomes. (wikipedia.org)
  • Cationic liposomes are manufactured similarly to liposomes. (wikipedia.org)
  • There are multiple processes that can be used to form cationic liposomes, such as sonication, extrusion, and vortexing. (wikipedia.org)
  • Microfluidics is a field that is currently being looked into for the purpose of forming cationic liposomes without the shear forces and damage associated with current methods. (wikipedia.org)
  • Cationic liposomes can deliver nucleic acids into the cell through an endocytotic pathway or through cell membrane fusion. (wikipedia.org)
  • Fusogenic cationic liposomes almost exclusively deliver nucleic acids through cell membrane fusion. (wikipedia.org)
  • The fusion between positively charged cationic liposomes and negatively charged cell surfaces efficiently delivers the DNA directly across the plasma membrane. (wikipedia.org)
  • Cationic liposomes in the lamellar phase deliver nucleic acids through endocytosis, specifically clathrin-mediated endocytosis (CME), caveolae-mediated endocytosis (CavME), and macropinocytosis. (wikipedia.org)
  • Secondly, non-viral vectors as well as cationic liposomes (LCs) will be modified and adapted to the micro-carriers environment. (fapesp.br)
  • The aim of the report is to study the formation and the physicochemical properties of liposomes and lipoplexes, in particular by fluorescence measurements, in order to determine the key factors for biological activity. (theses.fr)
  • Ce mémoire a pour objet d'étudier la formation et les propriétés physicochimiques des (liposomes puis des lipoplexes, en particulier par des mesures de fluorescence, afin de déterminer les paramètres clés pour l'activité biologique. (theses.fr)
  • Les études de fluorescence ont mis en évidence des corrélations entre la structure et les propriétés physicochimiques des liposomes. (theses.fr)
  • Ainsi la présence d'au moins une insaturation permet de fluidifier fortement les bicouches lipidiques, en outre la longueur des chaînes grasses joue sur la fusogénicité des liposomes. (theses.fr)
  • Synchrotron small-angle X-ray diffraction (SAXD) experiments showed that anionic liposomes made of dioleoylphosphatidylglycerol (DOPG) disintegrated the lamellar phase of lipoplexes. (unicam.it)
  • Whilst there is a large body of evidence looking at the design of cationic liposomes as transfection agents, correlates of formulation to function remain elusive. (aston.ac.uk)
  • Lipoplexes (LDs) have been prepared from DNA and positively charged vesicles composed of the helper lipid, dioleoyl L-α-phosphatidylethanolamine (DOPE) and either a dichain (DC) oxyethylated cationic lipid or their corresponding novel trichain (TC) counterpart. (brighton.ac.uk)
  • These cationic liposome-nucleic acid complexes are also referred to as lipoplexes. (wikipedia.org)
  • The most common non-vectors are called lipoplexes and polyplexes, which are formed by complexation of cationic liposome and cationic polymers with genetic materials, respectively. (fapesp.br)
  • Bottom line Collectively, the ip delivery of DFP-10825, TS shRNA conjugated with cationic liposome, displays a good antitumor activity without systemic undesirable occasions via the steady localization of TS shRNA for an adequate time and focus within the peritoneal cavity from the peritoneally disseminated individual ovarian cancer-bearing mice. (healthyconnectionsinc.com)
  • The present research explains the pharmacology and pharmacokinetic/pharmacodynamic account of DFP-10825, the TS shRNA-cationic liposome (lipoplex), inside a peritoneal disseminated human being ovarian malignancy (SKOV3-luc) xenograft in mice. (healthyconnectionsinc.com)
  • Dioleoylphosphatidylcholine (DOPC) and dioleoylphosphatidylethanolamine (DOPE), and O,O-ditetradecanoyl-N-(-trimethyl ammonioacetyl) diethanolamine chloride (DC-6-14), for planning of cationic liposome (known as lipoplex) were from NOF Inc. (Tokyo, Japan) and Nippon Good Chemical substances Inc. (Hyogo, Japan), respectively. (healthyconnectionsinc.com)
  • The planning of DFP-10825 from TS shRNA, and shRNA entrapment and retention from the DFP-10825 following the planning of cationic liposome (lipoplex). (healthyconnectionsinc.com)
  • Planning of cationic liposome (lipoplex) Cationic liposome made up of DOPE:DOPC:DC-16-4 (3: 2: 5 molar PF299804 percentage) was made by the technique of Abu Lila et al as explained previously.18 This lipoplex had not been constructed like a PEG modification. (healthyconnectionsinc.com)
  • Planning of TS shRNA-lipoplex (DFP-10825) For the planning of TS shRNA/cationic liposome complicated (TS shRNA-lipoplex, DFP-10825, Physique 1), TS shRNA and cationic liposome had been PF299804 mixed in a molar percentage of 2,000/1 (lipid:shRNA =2,000:1), as well as the combination was vigorously. (healthyconnectionsinc.com)
  • To this end new molecules , lipoplexes and polyplexes, have been created that have the ability to protect the DNA from undesirable degradation during the transfection process. (genetherapynet.com)
  • Most polyplexes consist of cationic polymers and their production is regulated by ionic interactions. (genetherapynet.com)
  • One large difference between the methods of action of polyplexes and lipoplexes is that polyplexes cannot release their DNA load into the cytoplasm , so to this end, co-transfection with endosome-lytic agents (to lyse the endosome that is made during endocytosis, the process by which the polyplex enters the cell ) such as inactivated adenovirus must occur. (genetherapynet.com)
  • Non-viral vectors based on magnetoplexes, lipoplexes and polyplexes for VEGF gene delivery into central nervous system cells. (ehu.eus)
  • Nanoparticles and corresponding nanoplexes -magnetoplexes, lipoplexes and polyplexes- were characterized in terms of size, zeta potential, polydispersity index, morphology and ability to bind, release and protect DNA. (ehu.eus)
  • Magnetoplexes showed the highest transfection efficiencies in C6, followed by lipoplexes, and in primary neuronal culture cells, followed by polyplexes. (ehu.eus)
  • The objective of this work was to study the formation of DNA-cationic vesicle complexes (lipoplexes). (dissertations.se)
  • The discovery that a high transfection efficiency can be achieved by employing multicomponent complexes at a lower-than-ever-before membrane charge density of lipoplexes was of primary significance. (unicam.it)
  • Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes. (umd.edu)
  • Multicomponent lipoplexes, incorporating from three to six lipid species simultaneously, presented a much higher transfection efficiency than binary lipoplexes, which are more commonly used for gene-delivery purposes. (unicam.it)
  • Of the lipoplexes prepared, those formulated with DSTAP showed higher transfection efficacy, however, the effect of buffer on transfection efficiency was formulation dependent. (aston.ac.uk)
  • The size of the cationic lipid and DNA complex (CL-DNA), or the lipoplex, is one of the important variations for consistency of gene transfection. (rsc.org)
  • In addition, this paper demonstrates that the gene transfection efficiency of lipoplex created in the PMRI is more reproducible. (rsc.org)
  • These influence strongly the proporties of the lipoplexes: low RC have slow kinetic of complexation and their transfection efficiency in vitro is sharply lower. (theses.fr)
  • Ceux-ci influencent fortement les propriétés des lipoplexes: d'une part les faibles RC aboutissent à une moins bonne complexation de l'ADN, d'autre part leur efficacité de transfection in vitro est très nettement inférieure. (theses.fr)
  • Each of the formulations was prepared by hydration in dH2O or phosphate buffer saline (PBS) to investigate the effect of buffer salts on lipoplex physicochemical characteristics and in vitro transfection. (aston.ac.uk)
  • Using PBS, rather than dH2O, to prepare the lipoplexes increased the size of vesicles in most of formulations and resulted in variation in transfection efficacies. (aston.ac.uk)
  • 5] Y. Hattori, S. Kawakami, K. Nakamura, F. Yamashita, and M. Hashida, "Efficient gene transfer into macrophages and dendritic cells by in vivo gene delivery with mannosylated lipoplex via the intraperitoneal route," J. Pharmacol. (fujipress.jp)
  • The purpose of this work is to present the key steps of the SAXS data treatment for the case of self-assembly and complexation of cationic lipid particles (vesicles or cubosomes) with plasmid DNA in the course of a rapid mixing stopped-flow process. (biologic.net)
  • A cationic lipid -based carrier is the most attractive nonviral solution for delivering plasmid DNA, shRNA, or drugs for pharmaceutical research and RNAi applications. (rsc.org)
  • Because the particle balance for the restorative substances conjugation, the free of charge TS shRNA within the formulation (TS shRNA-lipoplexes, DFP-10825) of 2.0 mg/kg (as shRNA) was checked by agarose gel electrophoresis. (healthyconnectionsinc.com)
  • A commonly used cationic phospholipid is DOTMA, and a commonly used fusogenic helper lipid is dioleoyl phosphatidylethanolamine (DOPE). (wikipedia.org)
  • Adding DOPE in addition to DOTMA promotes the endosomal escape of nucleic acids into the cytosol when the cationic lipid is fused to an endosome membrane. (wikipedia.org)
  • Due to the spacers used to combine the two symmetrical monomers, the synthesized gemini surfactant is cationic. (degruyter.com)
  • Before tail vein injection, lipoplexes were prepared as follows: 5 μg DNA and 25 μg DOTAP:Chol were diluted respectively in 50 μl 5% GS. (hormonessignaling.com)
  • DNA lipoplexes composed of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) or 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) in combination with 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) or 1,2-stearoyl-3-trimethylammonium-propane (DSTAP) were prepared by the lipid hydration method. (aston.ac.uk)
  • The present invention relates to a cationic lipid having one or more biodegradable groups located in a lipidic moiety (e.g., a hydrophobic chain) of the cationic lipid. (justia.com)
  • Several factors determine the balance between sulfation and glucuronidation (Mulder, Incorporation of Poly(Ethylene Glycol) Lipid into Lipoplexes 279 Figure 3 Effect on the zeta potential of the incorporation of 5 PEG-DODA into cationic lipoplexes [lipopolyamine RPR209120DOPE 11, ratio (mol) lipid DNA1410 in 150mM NaCl]. (forextradingwiz.com)
  • We also had a look at the lipoplex internalization pathway to profile the uptake characteristics. (iisc.ac.in)
  • The invention also relates to lipid particles comprising a neutral lipid, a lipid capable of reducing aggregation, a cationic lipid of the present invention, and optionally, a sterol. (justia.com)
  • CL-DNA size, in turn, may be controlled by factors such as the cationic lipid and DNA mixing order, mixing rate, and mixture incubation time. (rsc.org)
  • Felgner introduced the first cationic lipid used for gene delivery, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA). (wikipedia.org)
  • Cationic nano emulsion (CNE) is also explored as an alternative and relatively thermostable mRNA vaccine delivery vehicle. (awljournal.org)
  • The most common use of lipoplexes has been in gene transfer into cancer cells, where the supplied genes have activated tumor suppressor control genes in the cell and decrease the activity of oncogenes. (genetherapynet.com)
  • The approach taken was to study first the cationic vesicles by themselves. (dissertations.se)
  • La dernière partie de cette thèse a montré l'existence de divers types de lipoplexes selon le rapport de charge. (theses.fr)