• MBS7228917 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Y+L amino acid transporter 1 (SLC7A7) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • Retroviral cDNA libraries have been used in a number of functional screens to identify unique mammalian genes that regulate cellular responses ( 1 - 6 ). (pnas.org)
  • Here we describe an alternative method called cyclical packaging rescue (CPR) that uses direct repackaging of retroviral RNAs into new infectious virions to identify genes regulating functional responses in mammalian cells (Fig. 1 A ). In CPR, stably integrated helper-free retroviral libraries are recovered rapidly from mammalian cells as infectious helper-free retroviral supernatants 24 h after infection with adenoviruses expressing retroviral gag-pol and env genes. (pnas.org)
  • The genes identified in the screen include both modifiers that only partially protected RelA −/− cells against TNFα and a dominant-negative mutant of TNF receptor 1 (TNFR1) that was generated during the process of CPR. (pnas.org)
  • The encoding gene is adjacent to a paralogue ( serP2 ) with broad specificity for D- and L-small semipolar amino acids and glycine (see TC# 2.A.3.1.20). (tcdb.org)
  • To ensure that positive cDNA clones are successfully isolated from complex libraries in a single round of selection, functional screens using mammalian cells have been restricted to cellular assays in which the background of false-positive cells meeting a selection criterion is low, typically 1 in 10 5 false-positive cells. (pnas.org)
  • Disturbances in amino acid metabolism, which have been observed in Huntington's disease (HD), may account for the profound inanition of HD patients. (pnas.org)
  • This signaling cascade may be relevant to other diseases involving redox imbalance and deficits in amino acid metabolism. (pnas.org)
  • Abnormalities in amino acid metabolism have been frequently reported in HD, which may explain the weight loss and inanition observed during disease progression ( 5 ⇓ - 7 ). (pnas.org)
  • miR-122 expression is specific to the liver, where it has been implicated as a regulator of fatty-acid metabolism in mouse studies. (mirbase.org)
  • The absence of endoperoxide-containing products derived from 10,10-difluoroarachidonic acid has been thought to indicate the importance of a C-10 carbocation in PGG2 synthesis. (wikipedia.org)
  • The Mitochondrial Metallochaperone SCO1 Is Required to Sustain Expression of the High-Affinity Copper Transporter CTR1 and Preserve Copper Homeostasis. (jax.org)
  • We show here that the master regulator of amino acid homeostasis, activating transcription factor 4 (ATF4), is dysfunctional in HD, reflecting oxidative stress generated by impaired cysteine biosynthesis and transport. (pnas.org)
  • We show here that the master regulator of amino acid homeostasis, activating transcription factor 4 (ATF4), is dysfunctional in HD because of oxidative stress contributed by aberrant cysteine biosynthesis and transport. (pnas.org)
  • We reported a substantial depletion of cystathionine γ-lyase (CSE), the primary biosynthetic enzyme for the amino acid cysteine, in HD ( 8 ). (pnas.org)
  • Cysteine is a semiessential amino acid with multifaceted cellular functions ( 14 , 15 ). (pnas.org)
  • VRA is found about 50 amino acids from the amino terminus of the SU domain and contains two conserved cysteine residues. (hubpages.com)
  • VRB is found about 40 amino acids downstream of VRA and contains one conserved cysteine residue. (hubpages.com)
  • These may be expelled from the cell by the cationic transporter to be secreted in the urine, which is the primary route for symmetrical dimethylarginine clearance. (ahajournals.org)
  • a 16 amino acid sequence at the C-terminal end that is cleaved off by the viral protease (PR) after the virion has been released from the cell. (hubpages.com)
  • Chronic kidney disease (CKD) is a major public health concern affecting nearly 20 million people in the United States alone [ 1 ]. (hindawi.com)
  • Guidelines from the Kidney Disease Outcomes Quality Initiative (K/DOQI) place patients with CKD into one of five stages based on glomerular filtration rate (GFR) and are presented in Table 1 [ 1 , 15 ]. (hindawi.com)
  • Pavan B, Dalpiaz A. Prodrugs and endogenous transporters: are they suitable tools for drug targeting into the central nervous system? (springer.com)
  • It is involved in the conversion of arachidonic acid to prostaglandin H2, an important precursor of prostacyclin, among others. (wikipedia.org)
  • The PPR domain is found downstream of VRB and is approximately 50 amino acids long. (hubpages.com)
  • the membrane-binding domain consists of a series of amphipathic α helices with several hydrophobic amino acids exposed to a membrane monolayer. (wikipedia.org)
  • l -Citrulline supplementation resulted in considerable increase of the concentrations of amino acids and creatinine in the serum, and in their urinary excretion rates. (springer.com)