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  • hydroxyl groups
  • This activated oxygen species then proceeds to react with the substrate ultimately cleaving the carbon-carbon bond adjacent to the hydroxyl groups through the formation of an α-keto lactone intermediate. (wikipedia.org)
  • 1,2-CTD uses Fe3+ as a cofactor to cleave the carbon-carbon bond between the phenolic hydroxyl groups of catechol, thus yielding muconic acid as its product. (wikipedia.org)
  • In contrast, 2,3-CTD utilizes Fe2+ as a cofactor to cleave the carbon-carbon bond adjacent to the phenolic hydroxyl groups of catechol, thus yielding 2-hydroxymuconaldehye as its product. (wikipedia.org)
  • cleaves
  • strain Z1 directly cleaves the pyridine ring between N and position C-2 and further metabolizes the product via glutaric dialdehyde, and Bacillus sp. (biomedcentral.com)
  • reaction with dioxygen
  • 3-9 Although the reaction with dioxygen is thermodynamically favourable, the electronic configuration of the O 2 molecule creates a strong kinetic barrier that requires activation, which is useful to prevent any spontaneous reaction of O 2 with organic molecules. (rsc.org)
  • ferric iron
  • As a signature pattern for these enzymes we selected a region that includes a tyrosine residue which, in 3,4-PCD, has been shown [ 2 ], to be implicated in the binding of the ferric iron atom. (expasy.org)
  • reactivity
  • FeIII(L4)(dtbc)(H2O)] (dtbc = 3,5-di-tert-butylcatechol) was studied with respect to its dioxygen reactivity, and oxidative cleavage of the substrate was observed. (uu.nl)
  • genes
  • With respect to the initial step of biphenyl degradation, expression of all sets of ring hydroxylating dioxygenase genes (bphA, etbAl, etbA2) likewise is induced by biphenyl, and gene disruption experiments suggested that all these genes are involved in biphenyl degradation (Takeda et al. (alpfmedical.info)
  • carbon
  • The Sphingomonas macrogolitabida strain TFA is a gram-negative bacterium that is able to grow on tetralin (1,2,3,4-tetrahydronaphthalene) as the only carbon and energy source ( 14 ). (pubmedcentralcanada.ca)
  • A 4-aminopyridine-degrading enrichment culture utilized 4-aminopyridine as a carbon, nitrogen, and energy source, generating 4-amino-3-hydroxypyridine, 3,4-dihydroxypyridine, and formate as intermediates. (biomedcentral.com)
  • selective
  • Examples of recent gene duplication events in signaling as well as dioxygenase clusters are present, indicating selective gene family expansion as a relatively recent event in D. aromatica 's evolutionary history. (biomedcentral.com)
  • pathway
  • As shown in Fig. ​ Fig.1A, 1A , strain TFA metabolizes tetralin through a meta -cleavage pathway that involves an initial dioxygenation step (catalyzed by the enzymatic complex ThnA1A2A3A4), followed by a dehydrogenation step (catalyzed by ThnB) to produce 1,2-dihydroxytetralin ( 24 ). (pubmedcentralcanada.ca)
  • Two key metabolites of the meta-cleavage pathway, 5-formyl-2-hydroxy-6,6,8,8-tetramethylnona-2,4-dienoic acid and 6,6,8,8-tetramethyl-2-oxonon-4-enoic acid, were detected by LC-ESI-Q-TOF. (ncu.edu.tw)
  • utilize
  • Extradiol members utilize ferrous iron as the active redox state, and this center is commonly coordinated octahedrally through a 2-His-1-Glu motif with labile water ligands occupying empty positions. (wikipedia.org)
  • Among the culturable strains, strain 4AP-A could utilize 3,4-dihydroxypyridine as a growth substrate. (biomedcentral.com)
  • structural
  • At the center this barrel structure is a metal ion, most commonly ferrous iron, whose coordination environment is frequently provided by residues in two partially conserved structural motifs: G(X)5HXH(X)3-4E(X)6G and G(X)5-7PXG(X)2H(X)3N. (wikipedia.org)
  • This widespread structural motif is called the 2-His-1-carboxylate facial triad, and is found in many metalloproteins. (rsc.org)
  • metabolites
  • Octylphenol polyethoxylates (OPEOn) are surfactants and prone to be degraded into xenoestrogenic metabolites, such as octylphenol and OPEOn (n=1-2), are estrogenic-like compounds, and persistent to be degraded by bacteria in the environments. (ncu.edu.tw)
  • In the enrichment culture, 3,4-dihydroxypyridine and its metabolites including formate might be shared as growth substrates and maintain the enrichment culture, including these indispensable strains. (biomedcentral.com)
  • gene
  • Catechol 1,2-dioxygenase (EC 1.13.11.1 ) (gene catA or clcA). (expasy.org)
  • Chlorocatechol 1,2-dioxygenase (EC 1.13.11.1 ) (gene tfdC). (expasy.org)
  • suppressor gene can be implicated in the control of the cell routine, DNA MK-4827 synthesis, and DNA restoration aswell as apoptosis [2]. (cancer-colorectal.com)
  • Antibodies against p53 could be recognized in sera from tumor individuals, and a correlation between your expression of anti-p53 mutations and antibodies inside the gene continues to be founded [3]. (cancer-colorectal.com)
  • Anti-p53 antibodies have already been extensively studied since it has been proven that anti-p53 antibodies correlate towards the mutational position from the gene [3]. (cancer-colorectal.com)
  • Changes in the bacterial populations of 4-aminopyridine, 3,4-dihydroxypyridine, or formate/ammonium chloride enrichment cultures were monitored by denaturing gradient gel electrophoresis (DGGE) profiling of PCR-amplified 16S rRNA gene fragments. (biomedcentral.com)
  • atom
  • For example, in the α-ketoglutarate-dependent enzymes, one atom of dioxygen is incorporated into two substrates, with one always being α-ketoglutarate, and this reaction is brought about by a mononuclear iron center. (wikipedia.org)
  • sequence
  • However, especially in the case of homologous proteins showing relatively low sequence identities, such multiple 'isofunctional' enzymes may well have evolved distinct specificities for different sets of substrates (Gon^alves et al. (alpfmedical.info)
  • 2006). Even highly similar proteins may show subtle differences in their substrate preferences, as observed for EtbD1 (of pRHL1) and EtbD2 (of pRHL2), which share 97% sequence identity. (alpfmedical.info)
  • Biodegradability tests and partial sequence analysis of the enrichment culture indicated that 4-aminopyridine was mainly degraded via 3,4-dihydroxypyridine and that the metabolite is probably cleaved by 3-hydroxy-4-pyridone dioxygenase. (biomedcentral.com)