• The two enzymes were identified to be a part of two separate catechol dioxygenase families: 1,2-CTD was classified as an intradiol dioxygenase while 2,3-CTD was classified as an extradiol dioxygenase. (wikipedia.org)
  • It exhibited a half life of 30 min at 80 C and 81 min at 75 C, making it the most thermostable extradiol dioxygenase studied. (muni.cz)
  • A novel thermostable Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase (BphC_JF8) catalyzing the meta-cleavage of the hydroxylated biphenyl ring was purified from the thermophilic biphenyl and naphthalene degrader, Bacillus sp. (muni.cz)
  • The enzyme protocatechuate 3,4-dioxygenase (3,4-PCD) catalyzes the ring cleavage of protocatechuate (PCA) in Acinetobacter baylyi. (uni-ulm.de)
  • Comment: In MetaCyc pathway catechol degradation to HPD I (meta-cleavage, link ), dioxygenase xylE converts catechol to (2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate (also known as 2-hydroxymuconate 6-semialdehyde). (lbl.gov)
  • In MetaCyc pathway catechol degradation III (ortho-cleavage, link ), the 1,2-dioxygenase catA forms cis,cis-muconate, a cycloisomerase forms (+)-muconolactone, an isomerase converts this to (4,5-dihydro-5-oxofuran-2-yl)-acetate (also known as 3-oxoadipate enol lactone), and a hydrolase cleaves this to 3-oxoadipate. (lbl.gov)
  • This series of steps is part of protocatechuate para-cleavage, link , or catechol degradation II, link . (lbl.gov)
  • It is important to emphasize that the isolation of soil microorganisms on a mineral medium with benzoate made it possible to quickly assess the presence of one of two biodegradative pathways - ortho - or meta -cleavage of catechol. (sciforum.net)
  • The data obtained did not allow us to detect strains decomposing benzoate via the pathway of meta -cleavage, since there was no yellow coloration of the medium, characteristic of 2-hydroxymuconic semialdehyde formed as a result of meta -cleavage of catechol, a product of benzoate biodegradation. (sciforum.net)
  • An aromatic compound ring cleavage enzyme of catechol 1,2-dioxygenase was detected but catechol 2,3-dioxygenase was not detected in 2C6-43 T . A fatty acid profile with anteiso-C 15: 0 , iso-C 15: 0 and C 16: 0 as the major components supported the affiliation of strain 2C6-43 T to the genus Georgenia. (elsevierpure.com)
  • 2021. Separate upper pathway ring cleavage dioxygenases are required for growth of Sphingomonas wittichii strain RW1 on dibenzofuran and dibenzo-p-dioxin. (nih.gov)
  • Dehydrogenation of phenanthrene-cis-9,10-dihydrodiol to produce the corresponding diol, followed by ortho-cleavage of the oxygenated ring, produced 2,2'-diphenic acid. (afpm.org.my)
  • Dioxygenase NbaC cleaves the aromatic ring, yielding 2-amino-3-carboxymuconate 6-semialdehyde, a decarboxylase forms (2Z,4E)-2-aminomuconate semialdehyde, a dehydrogenase forms (2Z,4E)-2-aminomuconate, a deaminase forms (3E)-2-oxo-3-hexenedioate (also known as 2-oxalocrotonate), and a decarboxylase forms (2Z)-2-hydroxypenta-2,4-dienoate (HPD). (lbl.gov)
  • Comment: Dehydrogenase praB forms 2-hydroxymuconate, tautomerase praC forms (3E)-2-oxohex-3-enedioate (2-oxalocrotonate), and decarboxylase praD yields 2-hydroxypenta-2,4-dienoate (HPD). (lbl.gov)
  • p-Xylene-grown cells contained an inducible NAD+-dependent dehydrogenase which formed catechols from cis-p-toluate diol and the analogous acid diols formed from the other hydrocarbon substrates listed above. (rhea-db.org)
  • aldehyde dehydrogenase 1 family memb. (gsea-msigdb.org)
  • Catechol 2,3-dioxygenase (C23O) gene was found andamplified with the designed primers from the total DNA of C-14-1. (researchgate.net)
  • The result of Southern blot indicated that there isonly one C23O gene in the genome of C-14-1. (researchgate.net)
  • Complementation of the E. coli phr-1 mutation was observed, strongly suggesting that the yeast PHR1 gene encodes a. (shengsci.com)
  • Moreover, a dioxygenase gene, nidA3B3, was detected in Mycobacterium vanbaalenii PYR-1 as an alternate degradation pathway which could catalyze both the initial dihydroxylation of pyrene [ 11 ] to be pyrene cis-4,5-dihydrodiol, and an alternate detoxification pyrene pathway to be pyrene cis-1,2-dihydrodiol [ 12 ]. (omicsonline.org)
  • For all significantly differentially expressed microRNAs inside of the 2 groups, we now have produced gene sets from their expressed target genes. (pdpksignaling.com)
  • 2006. Microbial dioxygenase gene population shifts during polycyclic aromatic hydrocarbon biodegradation. (nih.gov)
  • 6 identified a gene cluster, hbpCAD , encoding the upper metabolic pathway of OPP which involves the transformation of OPP to 2-hydroxypenta-2,4-dienoateand benzoic acid (BA). (nature.com)
  • Catechol dioxygenases in microorganisms cleave catechol into cis - cis -muconic acid or 2-hydroxymuconic semialdehyde via the ortho- or meta- pathway, respectively. (preprints.org)
  • Characterization and optimization of C12O activity can assist in understanding the 2,4-DCP metabolic pathway in Pc UFB2 and its possible application in bioremediation strategies. (preprints.org)
  • The pcaH was significant in the β-ketoadipate pathway [9] where the pathway as it conserves biochemically, and is a major class of non-heme-iron containing dioxygenase [ 10 ]. (omicsonline.org)
  • Comment: In pathway I, dioxygenase kynA opens the non-aromatic ring, to N-formyl-L-kynureine, a hydrolase yields L-kynurenine (and formate), and a hydrolase yields anthranilate and L-alanine. (lbl.gov)
  • Comment: In MetaCyc pathway anthranilate degradation I ( link ), a dioxygenase cleaves off carbon dioxide and ammonia, leaving catechol. (lbl.gov)
  • This is part of a MetaCyc pathway for catechol degradation, link . (lbl.gov)
  • 1,2-CTD uses Fe3+ as a cofactor to cleave the carbon-carbon bond between the phenolic hydroxyl groups of catechol, thus yielding muconic acid as its product. (wikipedia.org)
  • They synthesized catechol 1, 2 - dioxygense enzyme which is unique its properties to transform catechol to cis, cis - muconic acid. (researchbib.com)
  • An enzyme that catalyzes the oxidation of catechol to muconic acid with the use of Fe3+ as a cofactor. (nih.gov)
  • Comment: There are two forms of anthranilate dioxygenase, 3-subunit antABC or 4-subunit andAabcd. (lbl.gov)
  • Two alpha-ketoglutarate dependent non-heme iron dioxygenases are responsible for the regulation of HIF-1 through hydroxylation of residues on the HIF-1a subunit. (umass.edu)
  • The enzyme is resistant to denaturation by various chelators and inhibitors (EDTA, 1,10-phenanthroline, H2O2, 3-chlorocatechol) and did not exhibit substrate inhibition even at 3 mM 2,3-dihydroxybiphenyl. (muni.cz)
  • An aerobic bacterial strain C-14-1 was isolated from an acrylic fiber wastewater. (researchgate.net)
  • Procedures for the purification of catechol 1,2-dioxygenase from extracts of Acinetobacter calcoaceticus strain ADP-96 are described. (shengsci.com)
  • Treatment of P. putida BG1 with nitrosoguanidine led to the isolation of a mutant strain which, when grown with fructose, oxidized both p-xylene and p-toluate to (-)-cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylic acid (cis-p-toluate diol). (rhea-db.org)
  • TIM barrel domain, Glyoxalase/fosfomycin resistance/dioxygenase domain, Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily [InterProScan]. (ntu.edu.sg)
  • Pre-Steady-State Kinetic Analysis of 2-Hydroxy-6-keto-nona-2,4-diene-1,9-dioic Acid 5,6-Hydrolase: Kinetic Evidence for Enol/Keto Tautomerization. (warwick.ac.uk)
  • Purification, Characterization, and Stereochemical Analysis of a C-C Hydrolase: 2-Hydroxy-6-keto-nona-2,4-diene-1,9-Dioic Acid 5,6-Hydrolase. (warwick.ac.uk)
  • The metal ligands and active site residues of extradiol dioxygenases are conserved although several amino acid residues found exclusively in enzymes which preferentially cleave bicyclic substrates are missing in BphC_JF8. (muni.cz)
  • One of these enzymes, known as the factor inhibiting HIF-1 (FIH-1) is responsible for hydroxylating residue Asn 803 on HIF-1a, preventing the transcription of hypoxia related genes controlled by HIF-1. (umass.edu)
  • Chemical and biochemical properties of 2-hydroxypentadienoic acid, a homolog of enolpyruvic acid. (warwick.ac.uk)
  • cis,cis-tetrachloromuconic_acid = 2 reactions were found. (brenda-enzymes.org)
  • The identification of 2,2'-diphenic acid in culture extracts indicates that phenanthrene was initially attacked through dioxigenation at C9 and C10 to give cis-9,10-dihydrodiol. (afpm.org.my)
  • Exploring the catalytic mechanism of the extradiol catechol dioxygenases. (warwick.ac.uk)
  • New metabolic pathways of phenanthrene and a better understanding of the phenoloxidases and dioxygenase mechanism involved in degradation of phenanthrene were explored in this research. (afpm.org.my)
  • 1. Fischer, D., Ebenau-Jehle, C. and Grisebach, H. Phytoalexin synthesis in soybean: purification and characterization of NADPH:2'-hydroxydaidzein oxidoreductase from elicitor-challenged soybean cell cultures. (qmul.ac.uk)
  • These bacteria subsequently employ 1,2-CTD in the last step of the degradation of aromatic compounds to aliphatic products. (wikipedia.org)
  • PAHs could be degraded by bacteria under aerobic conditions through the initial oxidation of the aromatic ring, which is catalyzed by the dioxygenase enzyme. (omicsonline.org)
  • Identification of metabolites from phenanthrene oxidation by phenoloxidases and dioxygenases of Polyporus sp. (afpm.org.my)
  • A blue white screening was established to identify mutants with acquired ability of 3,4-PCD to use catechol as substrate. (uni-ulm.de)
  • Analysis of 3,4-PCD activity toward catechol and PCA revealed further substitutions with changed substrate specificity. (uni-ulm.de)
  • In contrast, 2,3-CTD utilizes Fe2+ as a cofactor to cleave the carbon-carbon bond adjacent to the phenolic hydroxyl groups of catechol, thus yielding 2-hydroxymuconaldehye as its product. (wikipedia.org)
  • The second catechol hydroxyl group on carbon 3 (C3) is coordinated to Fe3+ after its deprotonation by the Tyr200 ligand. (wikipedia.org)
  • Polycyclic aromatic hydrocarbons (PAHs) are one class of organic pollutants that are considered the most hazardous due to their toxic, mutagenic, and carcinogenic properties [ 1 ]. (omicsonline.org)
  • It was hypothesized that there would be a difference in inhibition of FIH-1 from the other HIF-1 regulating enzyme, the prolyl hydroxylase domain-2 (PHD2), when testing a series of ten small molecule inhibitors. (umass.edu)
  • The ten inhibitors chosen fell into three classes: pyrones, pyridines, and catechols. (umass.edu)
  • Of these inhibitors, it was found that catechols produced a significant inhibitory difference between PHD2 and FIH, and may provide useful in further inhibitor design and synthesis work. (umass.edu)
  • The primary structure of BphC_JF8 exhibits less than 25% sequence identity to other 2,3-dihydroxybiphenyl 1,2-dioxygenases. (muni.cz)
  • Additional pathways are not included: the fate of 2-amino-5-oxocyclohex-1-enecarboxyl-CoA is not known ( link ), and anthraniloyl-CoA reductase (the only anaerobic route known, link ) has not been linked to sequence. (lbl.gov)
  • The enzyme contained 2 g-atoms of iron per mol of protein. (shengsci.com)
  • B, solute carrier relatives six member 2, solute carrier relatives 18 member one, and transcription factors and homeobox genes involved in neural crest derived cell de velopment, paired like homeobox 2a and 2b, GATA binding protein two and 3, heart and neural crest derivatives expressed two. (pdpksignaling.com)
  • docking protein 2 [Source:HGNC Symbo. (gsea-msigdb.org)
  • Comment: (2Z)-2-hydroxypenta-2,4-dienoate (HPD) is a common intermediate in the aerobic degradation of many aromatic compounds. (lbl.gov)