Catalyzes protein sequences protein residues. Medical search. Frequent questions

The horseshoe-shaped Hint domain contains a catalytic center for protein splicing, which involves both N and C-terminal residues. (rcsb.org)
Some of them encode proteins, some of them specify abundant RNAs like tRNAs and ribosomal RNAs, some of them are responsible for making a variety of small catalytic RNAs, and some unknown fraction may specify regulatory RNAs (e.g. lncRNAs). (blogspot.com)
Mutation of the catalytic cysteinyl residue in the active phosphatase domain reduced the in vitro activity of the mutant protein to less than 0.5% of wild type activity, while mutation of the corresponding cysteinyl residue of the inactive phosphatase-like domain had no effect on in vitro activity. (embl.de)
The prnD gene product of P. fluorescens RajNB11 that catalyzes the final step of production of pyrrolnitrin was characterized in the present study with regards to its stability and catalytic activity. (tubitak.gov.tr)
A complex, normally consisting of a catalytic and a regulatory subunit, which catalyzes the removal of a phosphate group from a serine or threonine residue of a protein. (ntu.edu.sg)
The catalytic subunit is an integral membrane protein with 6 transmembrane segments and it is postulated that the protein is anchored in the membrane at the N-terminal end. (unl.edu)
One of the methods that have been proposed is a multiobjective optimization, in which protein stability and catalytic activity are simultaneously optimized [ 4 , 5 ]. (hindawi.com)
Several methods have been proposed to design novel stable proteins, such as multi-objective optimization, in which protein stability and catalytic activity are simultaneously optimized. (hindawi.com)
The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. (unl.edu)
Our sequence analyses and structural modelling confirmed that these proteins possess mutarotase-like active sites with conserved catalytic residues. (cardiff.ac.uk)
Furthermore, based on sequence analysis it is proposed that Hjc_15-6 has a three-part catalytic motif corresponding to E-SD-EVK, and this motif may be common among other Hj-resolving enzymes originating from thermophilic bacteriophages. (iucr.org)
In this study a sixth xylanase gene, xyn11B, was isolated which encodes a 357-residue modular enzyme, designated Xyn11B, comprising a glycoside hydrolase family 11 catalytic domain appended to a C-terminal X-14 module, a homologue of which binds to xylan. (omicsdi.org)
Though similar to ClpP proteins, this does not contains the highly conserved catalytic triad of Ser-type proteases (Ser-His-Asp). (or.jp)

This control is propagated through the alpha-subunit of prolyl 4-hydroxylase where protein levels were previously shown to rise fivefold with increasing cell density. (embl.de)
A single protein subunit is highlighted. (transectscience.org)
The amino-terminal sequence of β-galactosidase, the α-peptide involved in α-complementation, participates in a subunit interface. (wikidoc.org)
In humans there exist five isoforms of the B56 type regulatory subunit and they bind to their interacting proteins through a conserved LxxIxE motif. (eu.org)
Protein dephosphorylation by the PP2A phosphatase is mainly achieved through the interaction of its regulatory subunit with the associated proteins. (eu.org)

Copper ions bind to biomolecules (e.g., peptides and proteins) playing an essential role in many biological and physiological pathways in the human body. (mdpi.com)
If you look at the coding sequence of a gene you can predict all of the peptides that will be produced by digesting with various proteases. (blogspot.com)
The PPS1 protein was expressed in Escherichia coli, and the protein was shown to catalyze the hydrolysis of p-nitrophenyl phosphate, dephosphorylate phosphotyrosyl, and phosphothreonyl residues in synthetic diphosphorylated peptides and to inactivate the human ERK1 protein. (embl.de)
4 ] used a multi-objective optimization to build new stable peptides based on the villin headpiece (VH) sequence, which is known to be stable in vitro . (hindawi.com)
Short polypeptides, containing less than 20-30 residues, are rarely considered to be proteins and are commonly called peptides, or sometimes oligopeptides. (transectscience.org)

First, peptide deformylase deformylates it, converting the residue back to a normal methionine. (wikipedia.org)
The prototypical fMet-containing oligopeptide is N -formylmethionine-leucyl-phenylalanine (FMLP) which activates leukocytes and other cell types by binding with these cells' formyl peptide receptor 1 (FPR1) and formyl peptide receptor 2 (FPR2) G protein coupled receptors (see also formyl peptide receptor 3 ). (wikipedia.org)
The derived amino acid sequence of Ov-phy-1 encoded a peptide that was most similar to the two Caenorhabditis elegans prolyl 4-hydroxylase homologues and to the isoform II enzymes of vertebrates. (embl.de)
it catalyzes cleavage of the peptide bond on the carbonyl side of lysine (K) and arginine (R) residues producing peptide fragments that all end with lysine and arginine. (blogspot.com)
For example, one of the predicted fragments of the human serum albumin protein is FKDLGEENFK (ends in lysine=K). This has an exact molecular mass of 1226.59 so if you find a peptide of that size in your mixture then it probably means that human serum albumin was present. (blogspot.com)
In practice you want to identify several unique peptide masses for each protein just to be sure. (blogspot.com)
The IgAs shown specific binding of the artificial 416C433 Aripiprazole (D8) peptide mimetics reliant on recognition from the Compact disc4 binding residues situated in this area. (siamtech.net)
Crystallography and mutagenesis research indicate which the 421C433 peptide area provides essential proteins forming the Compact disc4BS [17C20]. (siamtech.net)
The individual amino acid residues are bonded together by peptide bonds and adjacent amino acid residues. (transectscience.org)
Sometimes proteins have non-peptide groups attached, which can be called prosthetic groups or cofactors. (transectscience.org)
We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)
The method's focus is to globally detect mass differences, not to assign peptide sequences or modifications to individual spectra. (lu.se)
The goal is to assign acquired spectra to known peptide sequences and potential co- and post-translational modifica- tions. (lu.se)
The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
This pD dependence is due to labile protein deuterons in acidic side-chains and surface peptide groups, which exchange rapidly with water deuterons. (lu.se)

To design, researchers used molecular structure and sequence homology to guide selection of amino acid residues for creating new-to-nature enzymes that catalyze the desired chemistry. (energy.gov)
However, analogous proteins may have structural homology although this is not a prerequisite. (hindawi.com)
Newly-identified distinct sequences with homology to the hedgehog/intein superfamily, called bacterial intein-like (BIL) domains, often do not have Cys, Ser, or Thr as the obligatory nucleophilic residue found in inteins. (aalto.fi)
Firefly luciferase shows extensive sequence homology with a number of enzymes that utilize ATP in adenylation reactions. (rcsb.org)

Availability of genome sequences facilitated the identification of pneumococcal surface proteins bearing characteristic motifs such as choline-binding proteins (Cbp) and peptidoglycan binding (LPXTG) proteins. (biomedcentral.com)
High-throughput sequence analysis of total RNA prepared from cultures with large dsRNAs identified two novel RNA viruses with genome sequences of approximately 9.3 kbp, which were named Fusarium virguliforme dsRNA mycovirus 1 and Fusarium virguliforme dsRNA mycovirus 2. (illinois.edu)

EC 2.4.2.26) catalyzes the transfer of UDP-xylose to serine residues within XT recognition sequences of target proteins. (wikipedia.org)
To block the very formation of these side chains xylosyltransferase (XYLT1) which attaches xylose to a serine of the protein core as initiation for glycosaminoglycan chain extension, was targeted by a class of designed DNA molecules. (wikipedia.org)
They remove the phosphate group from both phospho-tyrosine and phospho-serine/threonine residues. (embl.de)
The protein of P. fluorescens was found to have a better folded structure, more serine residues in the active site, and better active site properties and isoelectric point value in comparison to PrnD proteins of Burkholderia and Serratia. (tubitak.gov.tr)

The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. (unl.edu)
The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. (unl.edu)
Database The resonance assignments and structure coordinates have been deposited in BMRB (18653) and RCSB (2LWY) Bacterial intein-like (BIL) domains belong to Hint superfamily but lack an essential nucleophilic residue (Cys/Ser/Thr) for protein splicing, which is found in all inteins. (aalto.fi)
Protein phosphatase 2A (PP2A) belongs to the superfamily of phosphoprotein phosphatases (PPPs) and catalyzes protein dephosphorylation by hydrolyzing Ser/Thr-linked phosphate ester bonds ( Heroes,2013 ). (eu.org)

If the protein is expressed in many different species then the exon sequences will be conserved and it's easy for a computer program to identify the gene. (blogspot.com)
The tough part comes when the algorithm predicts a new protein-coding gene based on an open reading frame spanning several presumed exons. (blogspot.com)
The gene-finding algorithms tend to over-predict the number of protein-coding genes and subsequent annotation leads to a "finished" genome sequence that eliminates all the false positives. (blogspot.com)
The most obvious way of confirming a potential protein-coding gene is to find and confirm synthesis of a functional protein. (blogspot.com)
This technique only works if you have a reliable gene sequence and it only works on the genome level if you have a "finished" genome sequence, properly annotated so you can identify all potential reading frames. (blogspot.com)
The PPS1 gene of Saccharomyces cerevisiae codes for a dual specificity protein phosphatase with a role in the DNA synthesis phase of the cell cycle. (embl.de)
The prnD gene was amplified from RajNB11, sequenced, translated, and 3-D modeled to its protein structure. (tubitak.gov.tr)
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. (nih.gov)
A sequencing platform was used for genome assembly and gene expression. (bvsalud.org)
The unigene sequences were annotated and functionally classified by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. (bvsalud.org)
The sequence of amino acid residues in a protein is defined by the sequence of a gene which is encoded in the genetic code. (transectscience.org)
such methylation can be involved in modification of heavy metals , regulation of gene expression , regulation of protein function, and RNA processing . (explained.today)
Methylation can modify heavy metals, regulate gene expression, RNA processing and protein function. (explained.today)
Beta-ketoacyl-ACP synthase I of Escherichia coli: nucleotide sequence of the fabB gene and identification of the cerulenin binding residue. (genebiosystems.com)
Histone acetylation is certainly catalyzed by histone acetyltransferases (HATs) and histone deacetylation with a.Deacetylation is restrictive, however the results vary with this gene getting regulated. (mergullo.net)

These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. (unl.edu)
Many proteins are enzymes that catalyze biochemical reactions and are vital to metabolism. (transectscience.org)

A minority of preimmune antibodies created without contact with HIV bind the 421C433 epitope of gp120 [23C25] and check out catalyze the hydrolysis of gp120 [26,27]. (siamtech.net)
Chitinases are glycosyl hydrolases that catalyze the hydrolysis of the β-1,4 linkages in complex carbohydrates and those that contain GlcNAc. (researchgate.net)
β-galactosidase , also called lactase , beta-gal or β-gal , is a glycoside hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond . (wikidoc.org)
[10] The active site of β-galactosidase catalyzes the hydrolysis of its disaccharide substrate via "shallow" (nonproductive site) and "deep" (productive site) binding. (wikidoc.org)

Solvent- accessible surface representation colored by residue type (acidic residues red, basic residues blue, polar residues green, nonpolar residues white). (transectscience.org)
The pocket is essentially linear, with a basic patch interacting with the acidic residues in the C-terminal end of the motif and the hydrophobic patch interacting with the two key hydrophobic residues of the motif. (eu.org)
A higher degree of flexibility is observed in the intervening and surrounding positions and a stronger binding affinity is observed when the residues at the position P2 or P7-P9 were either phosphorylated or an acidic residue is already present, as the negative charge engages with the basic patch of B56 ( Wang,2016 ). (eu.org)

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (rndsystems.com)

Furthermore proteins could be acetylated at lysine residues as well as the related enzymes are for historic reasons referred to as histone acetyltransferases (HATs) and histone deacetylases (HDACs). (gasyblog.com)
The most prevalent protein methylations affect arginine and lysine residue of specific histones. (explained.today)

Likely via a radical-pair mechanism 12 , T 1 reacts within microseconds to the signaling state, characterized by a covalent thioadduct between a highly conserved cysteine residue in the LOV photosensor and the C4a atom of the flavin isoalloxazine ring system. (nature.com)
A highly conserved glutamine residue in strand Iβ is situated immediately adjacent to the flavin and has been identified as instrumental in reading out the flavin N5 position and eliciting the downstream transitions. (nature.com)
Aside from the obvious highly conserved genes for ubiquitous RNAs (rRNA, tRNAs etc.), protein-coding genes are the easiest to recognize from looking at a genome sequence. (blogspot.com)

abstract = "Protein splicing in trans by split inteins has become a useful tool for protein engineering in vivo and in vitro. (aalto.fi)

Like other biological macromolecules such as polysaccharides and nucleic acids, proteins are essential parts of organisms and participate in virtually every process within cells. (transectscience.org)
These superhelical structures present an extensive solvent-accessible surface that is well suited to binding large substrates such as proteins and nucleic acids. (eu.org)

The peptidyl-prolyl cis / trans isomerase (PPIase) class of proteins is present in all known eukaryotes, prokaryotes, and archaea, and it is comprised of three member families that share the ability to catalyze the cis / trans isomerisation of a prolyl bond. (biomedcentral.com)
The peptidyl-prolyl cis / trans isomerase (PPIase) class of proteins is traditionally comprised of three distinct protein families, the cyclophilins (cyclosporin A binding proteins), FKBPs (FK506 binding proteins) and parvulins, that are linked by their shared ability to catalyse the bond preceding a proline residue between its cis and trans forms. (biomedcentral.com)

It is specifically used for initiation of protein synthesis from bacterial and organellar genes, and may be removed post-translationally . (wikipedia.org)
fMet plays a crucial part in the protein synthesis of bacteria, mitochondria and chloroplasts . (wikipedia.org)
It is not used in cytosolic protein synthesis of eukaryotes , where eukaryotic nuclear genes are translated . (wikipedia.org)
fMet is a starting residue in the synthesis of proteins in bacteria, and, consequently, is located at the N -terminus of the growing polypeptide . (wikipedia.org)
The mitochondria of eukaryotic cells, including those of humans, and the chloroplasts of plant cells also initiate protein synthesis with fMet. (wikipedia.org)
Given that mitochondria and chloroplasts have this initial protein synthesis with fMet in common with bacteria, this has been cited as evidence for the endosymbiotic theory . (wikipedia.org)
Researchers addressed this challenge by developing a cell-free DNA assembly and protein synthesis platform, allowing researchers to rapidly screen 1000's of sequence defined enzyme mutants in iterative design-build-test-learn cycles. (energy.gov)
pps1Delta mutant strains did not differ from PPS1 strains under any of the conditions tested, but overexpression of the PPS1 protein in S. cerevisiae led to synchronous growth arrest and to aberrant DNA synthesis. (embl.de)
Shortly after or even during synthesis, the residues in a protein are often chemically modified by post-translational modification, which alters the physical and chemical properties, folding, stability, activity, and ultimately, the function of the proteins. (transectscience.org)
Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. (genebiosystems.com)

Although DOPAL synthase and DDC share high sequence similarity, they catalyze rather different chemical reactions. (biomedcentral.com)
The deduced amino acid sequence of PPS1p shows similarity with protein-tyrosine phosphatases (PTPases) and is most closely related to a subfamily of PTPases that are capable of dephosphorylating phosphoseryl and phosphothreonyl residues as well as phosphotyrosyl residues. (embl.de)
on the other hand, there are many cases of nearly identical structures known, sharing no sequence similarity at all [ 3 ]. (hindawi.com)
The sequence similarity among these different repeats or domains is low, however they exhibit considerable structural similarity. (eu.org)

It is about 140 residues long and it has been suggested that this domain will be involved in tRNA recognition. (embl-heidelberg.de)
The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. (nih.gov)
2005) reported the recognition of indole derivatives that focus on SR protein and thereby impact alternate splicing. (gasyblog.com)

The technique relies on mass-spectrometry and the existence of a well-annotated genome sequence [ Genomics, Proteomics and Mass Spectrometry ] [ Biochemistry and Mass Spectrometry ]. (blogspot.com)
To your knowledge none of the few small-molecule inhibitors of pre-mRNA splicing have already been utilized to isolate the stalled splicing complexes for even more evaluation like the dedication of proteins structure by mass spectrometry. (gasyblog.com)
Methods commonly used to study protein structure and function include immunohistochemistry, site-directed mutagenesis, X-ray crystallography, nuclear magnetic resonance and mass spectrometry. (transectscience.org)

There are about 20,000 potential protein-coding genes in the human genome. (blogspot.com)
Over the years, the number of protein-coding genes in the human genome has dropped from the initial reports of about 30,000 to about 20,000. (blogspot.com)

Removing intervening sequences from an initial RNA transcript is catalyzed from the spliceosome a big complex comprising five small nuclear (sn) RNAs and a lot more than 150 proteins. (gasyblog.com)

Addition of this xylose to the core protein is required for the biosynthesis of the glycosaminoglycan chains characteristic of proteoglycans. (wikipedia.org)
Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan. (unl.edu)
This process, catalyzed by enzymes such as caffeoyl-CoA O-methyltransferase , is a key reaction in the biosynthesis of lignols , percursors to lignin , a major structural component of plants. (explained.today)

Hence it is plausible that such actions might work on RNA and proteins conformations or on post-translational changes states of protein through the splicing routine. (gasyblog.com)
The method can be used for automated detection of chemical/post- translational modifications, quality control of experiments and labelling approaches, and to control the modification settings of protein identification tools. (lu.se)
More recently, the concept of lysosomal storage disease has been expanded to include deficiencies or defects in proteins necessary for the normal post-translational modification of lysosomal enzymes (which themselves are often glycoproteins), activator proteins, or proteins important for proper intracellular trafficking between the lysosome and other intracellular compartments. (medscape.com)

Structural and biochemical comparisons between DOPAL synthase and DDC in Drosophila identified that the key active site residue, Asn192, in insect DOPAL synthase can be used to distinguish all available insect DOPAL synthases from DDC sequences [ 15 ]. (biomedcentral.com)
Together with ubiquitination and phosphorylation , methylation is a major biochemical process for modifying protein function. (explained.today)
Protein structures are invaluable for biochemical studies and for mechanical or molecular mechanics calculations have become the understanding of how proteins work. (lu.se)
The most common strong alternative and complement to experimental methods to methods to obtain protein structures are X-ray crystallography obtain insight in biochemical processes. (lu.se)

UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. (unl.edu)
STS, which is localized in the endoplasmic reticulum, catalyzes desulfation of 3beta-hydroxysteroid sulfates. (medscape.com)

The addition of the formyl group to methionine is catalyzed by the enzyme methionyl-tRNA formyltransferase . (wikipedia.org)
However, transformylase will catalyze the addition of the formyl group to methionine only if methionine has been loaded onto tRNA fMet , not onto tRNA Met . (wikipedia.org)
There are 26085 Arg_tRNA_synt_N domains in 26083 proteins in SMART's nrdb database. (embl-heidelberg.de)
Taxonomic distribution of proteins containing Arg_tRNA_synt_N domain. (embl-heidelberg.de)
The complete taxonomic breakdown of all proteins with Arg_tRNA_synt_N domain is also avaliable . (embl-heidelberg.de)
Click on the protein counts, or double click on taxonomic names to display all proteins containing Arg_tRNA_synt_N domain in the selected taxonomic class. (embl-heidelberg.de)

The motif is conserved in essential proteins throughout the eukaryotic domain of life and also in human viruses, suggesting that the motifs are required for basic cellular function. (eu.org)

DOPAL synthase is a member of the insect DDC-like protein family. (biomedcentral.com)
Early studies reported that DOPAL synthase-like protein, alpha methyldopa resistant (AMD-r), in Drosophila is resistant to the toxic compound, AMD. (biomedcentral.com)
Cellulose synthase (CESA) catalyzes the polymerization reaction of cellulose using UDP-glucose as the substrate. (unl.edu)
Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. (unl.edu)
DPG_synthase is involved in protein N-linked glycosylation. (unl.edu)

Certain very abundant ribosomal proteins can be the sacrificial substrates targeted for degradation at the onset of the downshift. (go.jp)
When enzymes bind multiple substrates, such as dihydrofolate reductase (shown right), enzyme kinetics can also show the sequence in which these substrates bind and the sequence in which products are released. (wikipedia.org)

Tyrosine specific protein phosphatases (PTPases) contain two conserved cysteines, the second one has been shown to be absolutely required for activity. (embl.de)

Methylmalonyl-CoA mutase (EC 5.4.99.2) catalyzes a reversible isomerization between L-methylmalonyl-CoA and succinyl-CoA. (nih.gov)
Reversible protein phosphorylation is an essential regulatory mechanism in eukaryotes. (eu.org)

In convergent evolution, nonhomologous proteins evolve in separate biological contexts to catalyze the same or similar reactions. (hindawi.com)

Beta-galactosidase has many homologues based on similar sequences. (wikidoc.org)

This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. (unl.edu)
Hs SQM also catalyzed the mutarotation of various aldohexoses with an equatorial 2-hydroxy group, including D-galactose, D-glucose, D-glucose-6-phosphate, and D-glucuronic acid, but not D-mannose. (cardiff.ac.uk)
Three possible representations of the three-dimensional structure of the protein triose phosphate isomerize. (transectscience.org)

As recently proposed 25 , glutamine reorientation, and signal propagation may be aided by transient rearrangements of two conserved asparagine residues that coordinate the pteridin portion of the flavin. (nature.com)

The protein was recognized by rabbit antibodies raised against rat P-450 2A1, and a rabbit antiserum against the P-450 2A6 preparation was also prepared. (aspetjournals.org)
Purified P-450 2A6 catalyzed coumarin 7-hydroxylation and 7-ethoxycoumarin O-deethylation at rates similar to those measured in the human liver sample used to prepare P-450 2A6, and these two microsomal activities were strongly inhibited by the antibodies. (aspetjournals.org)
Equivalent amounts of protein were separated on SDS-PAGE, transferred onto PVDF membranes and then incubated with main antibodies (Table 1). (healthanddietblog.info)

[9] Instead, fMet-containing oligopeptides and proteins appear to be released by the mitochondria of damaged tissues as well as by damaged bacteria, and can thus qualify as an "alarm" signal, as discussed in the Danger model of immunity. (wikipedia.org)

Search proteins in UniProtKB for this molecule. (rhea-db.org)

Approximately 100 amino acids, corresponding to the middle region of class I/II HCPs, are absent, although N- and C-terminal regions and residues for metallocluster ligands are conserved among all HCP classes. (nature.com)

Mass-spectrometric evaluation of affinity-purified stalled spliceosomes indicated how the intermediates differ in proteins structure both from one another and from previously characterized indigenous A and B splicing complexes. (gasyblog.com)
Proteins can also work together to achieve a particular function, and they often associate to form stable protein complexes. (transectscience.org)

That still left thousand of putative genes with no evidence that they actually make a protein. (blogspot.com)
NdvC_like proteins in this family are putative bacterial beta-(1,6)-glucosyltransferase. (unl.edu)

Proteoglycans consist of one relatively small protein core and attached large glycosaminoglycan side chains. (wikipedia.org)
The most similar archaeal sequences are separate chains, such as AF2215 and AF2219 of Archaeoglobus fulgidus, that correspond roughly to the first 500 and last 130 residues, respectively of known methylmalonyl-CoA mutases. (nih.gov)
Inteins require Cys, Ser or Thr at the first residue of the C-terminal flanking sequence because a thiol or hydroxyl group in the side chains is a nucleophile indispensable for the trans-esterification step during protein splicing. (aalto.fi)
Proteins are large biomolecules, or macromolecules, consisting of one or more long chains of amino acid residues. (transectscience.org)

On the other hand, BmTsp.A inhibits the expression of BmPTEN and BmPkc through the phosphatidylinositol 3 kinase (PI3K)/protein kinaseB(AKT) signaling pathway, thus affecting the regulation of apoptosis. (bvsalud.org)
In addition, eHSP90 induced ER stress in fibroblasts the phosphoinositide-4,5-bisphosphate 3-kinase (PI3K)-protein kinase B (AKT) signaling pathway, which could become blocked from the PI3K/AKT inhibitor LY294002, and blockade of eHSP90 by 1G6-D7 markedly inhibited ER stress in the model, indicating preventive and restorative applications. (healthanddietblog.info)

Expressed sequence tag (EST) analysis and developmental polymerase chain reaction (PCR) studies demonstrated that Ov-phy-1 was expressed in L3 and adult parasites. (embl.de)
There exist two types of convergent evolution: (1) mechanistic analogs that uses the same mechanisms to perform related reactions and (2) transformational analogs catalyzing exactly the same reaction. (hindawi.com)
For metal- powerful methods to obtain and compare reaction and activation loproteins, a third method to obtain local information about the energies for suggested enzyme mechanisms and they can also provide atomic details about the protein dynamics. (lu.se)

The N -terminal fMet is removed from majority of proteins, both host and recombinant, by a sequence of two enzymatic reactions. (wikipedia.org)
Inteins possess two different enzymatic activities, self-catalyzed protein splicing and site-specific DNA cleavage. (rcsb.org)

Encodes a ClpP-related sequence. (or.jp)

Hydrophobicity was one of the most important physicochemical properties, due to the fact that it is involved in protein interactions, for example, by forming hydrophobic cores. (hindawi.com)
L..[IVLWC].E). One of, but not both, the amino acids in position P1 or P4 of the motif can be a variable hydrophobic residue for B56 binding, while P6 has the invariant glutamic acid. (eu.org)

Taken with the present results, this suggests that the potential use of inhibitors of prolyl 4-hydroxylase to control excessive collagen deposition in pathological fibrosis may be limited by the need to maintain continuous inhibition of collagen hydroxylation so as to facilitate intracellular degradation of the accumulated protein. (embl.de)

Furthermore, structural analysis of Mm HCP C67Y and characterization of several Mm HCP variants with a Cys67 mutation reveal the significance of Cys67 in protein structure, metallocluster binding and hydroxylamine reductase activity. (nature.com)

These proteins are involved in the elongation of the glucan chain of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues. (unl.edu)

Proteins differ from one another primarily in their sequence of amino acids, which is dictated by the nucleotide sequence of their genes, and which usually results in protein folding into a specific three-dimensional structure that determines its activity. (transectscience.org)

Using a fluorescent sulfhydryl probe as well as a sulfhydryl inhibitor, one observes a strong cell density response, supporting the hypothesis that cellular redox potential could alter protein stability. (embl.de)
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
The protein from P. fluorescens was found to be basic, hydrophilic with higher thermal stability, and easily separated after expression and purification. (tubitak.gov.tr)
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. (genebiosystems.com)

BmTsp.A (Bombyx mori Tetraspanin A) is one of the four transmembrane proteins which are capable to regulate multiple aspects of the immune response and are involved in various stages of viral invasion of the hosts. (bvsalud.org)

The modeled protein of the test organism was compared to PrnD proteins of Burkholderia sp. (tubitak.gov.tr)

Although the genome of F. virguliforme was sequenced, there were limited studies focused on the PCWDEs of F. virguliforme. (omicsdi.org)
While citrinin, fusaric acid, and radicicol induced foliar chlorosis and wilting, Soybean mosaic virus (SMV)-mediated overexpression of F. virguliforme necrosis-inducing secreted protein 1 (FvNIS1) induced SDS foliar symptoms that mimicked the development of foliar symptoms in the field. (illinois.edu)

The amino-terminal amino acid sequence of the protein (first 13 residues) matched that of the reported cDNA exactly. (aspetjournals.org)

PP2A-mediated protein dephosphorylation is involved in a broad range of cellular processes including cell-cycle progression, cytoskeletal dynamics, and growth factor signalling. (eu.org)

Fig. 1: Photochemistry of light-oxygen-voltage receptors and sequences of proteins under study. (nature.com)

Proteins perform a vast array of functions within organisms, including catalyzing metabolic reactions, DNA replication, responding to stimuli providing structure to cells and organisms, and molecules from one location to another. (transectscience.org)
β-galactosidase can catalyze two different reactions in organisms. (wikidoc.org)

Because fMet is present in proteins made by bacteria but not in those made by eukaryotes (other than in bacterially derived organelles), the immune system might use it to help distinguish self from non-self. (wikipedia.org)

Human P-450 2A6 did not catalyze testosterone 7 alpha-hydroxylation, a characteristic activity of the related rat P-450 2A1 protein. (aspetjournals.org)

Co-expression of Ov-phy-1 with the O. volvulus homologue of protein disulfide isomerase in a baculovirus system resulted in the production of enzymatically active O. volvulus prolyl 4-hydroxylase. (embl.de)

To build, researchers used site saturation mutagenesis to create a DNA library of enzyme variants containing mutations at the identified residues. (energy.gov)
First, the sequences carrying point mutations are modeled on a given template structure, and subsequently, molecular dynamics simulations are carried out for 10 ns. (hindawi.com)

They recognize long asymmetric nucleotide sequences and cleave both DNA strands in a monomer form. (rcsb.org)
An example of enzymes that bind a single substrate and release multiple products are proteases , which cleave one protein substrate into two polypeptide products. (wikipedia.org)
Amino-acid sequence and structure comparison suggested that the enzyme belongs to a group of enzymes classified as archaeal Holliday junction-resolving enzymes, which are typically divalent metal ion-binding dimers that are able to cleave X-shaped dsDNA-Holliday junctions (Hjs). (iucr.org)

The precise regulations of pre-RC protein levels and assembly are effective ways to prevent reassembly of de novo MCM2-7 onto the replicated origins to re-license and re-replicate the genomic DNA in the subsequent phases of the same cell cycle ( Figure 1) . (intechopen.com)
In epipelagic SAR324 ecotypes, we observed the presence of two types of proton-pumping rhodopsins, as well as genomic, transcriptomic, and ecological evidence for active photoheterotrophy, based on xanthorhodopsin-like light-harvesting proteins. (biomedcentral.com)

Since fMet is present in proteins made by mitochondria and chloroplasts, more recent theories do not see it as a molecule that the immune system can use to distinguish self from non-self. (wikipedia.org)
In Escherichia coli , amino acid starvation leads to the accumulation of an unusual molecule, polyphosphate (polyP), a linear polymer of many hundreds of orthophosphate residues. (go.jp)
Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. (unl.edu)

Digestion breaks the proteins down for use in the metabolism. (transectscience.org)
Briefly, calibrators, quality controls, a matrix blank, blind QCs, and NHANES samples were prepared by performing a protein digestion using pronase to release the chlorinated tyrosine biomarkers. (cdc.gov)
After digestion, any undigested proteins were removed by protein precipitation. (cdc.gov)

Cellulose is an aggregate of unbranched polymers of beta-1,4-linked glucose residues, which is an abundant polysaccharide produced by plants and in varying degrees by several other organisms including algae, bacteria, fungi, and even some animals. (unl.edu)
A). stress inhibitor tauroursodeoxycholate (TUDCA) or glucose-regulated protein 78?kDa (GRP78) depletion significantly abrogated the effect of eHSP90 on ER stress and fibroblast activation. (healthanddietblog.info)

Polymorphonuclear cells can bind proteins starting with fMet, and use them to initiate the attraction of circulating blood leukocytes and then stimulate microbicidal activities such as phagocytosis . (wikipedia.org)

Anatomy8

Organisms18

Chemicals and Drugs69

Analytical, Diagnostic and Therapeutic Techniques and Equipment47

Phenomena and Processes91

Disciplines and Occupations4

Technology, Industry, Agriculture1

Information Science31

Health Care9

Catalytic13

Subunit5

Peptides5

Peptide15

Homology4

Genome Sequences2

Serine4

Superfamily4

Gene15

Enzymes that catalyze2

Hydrolysis4

Acidic3

Recombinant proteins1

Lysine2

Highly conserved3

Abstract1

Nucleic acids2

Peptidyl-prolyl c2

Synthesis10

Similarity4

Recognition3

Mass Spectrometry3

Human genome2

Transcript1

Biosynthesis3

Post-translational3

Biochemical4

Endoplasmic reticulum2

TRNA6

Essential proteins1

Synthase5

Substrates2

Phosphatases1

Reversible2

Biological1

Similar sequences1

Phosphate3

Asparagine1

Antibodies3

Oligopeptides1

UniProtKB1

Ligands1

Complexes2

Putative2

Chains4

PI3K2

Reaction3

Enzymatic2

Encodes1

Hydrophobic2

Inhibitors1

Mutation1

Elongation1

Nucleotide sequence1

Stability4

Transmembrane1

Organism1

Virguliforme2

Amino-terminal1

Dephosphorylation1

Receptors1

Reactions2

Eukaryotes1

Characteristic1

Homologue1

Mutations2

Cleave3

Genomic2

Molecule3

Digestion3

Glucose2

Bind1