This enzyme catalyses the following chemical reaction: S-adenosyl-L-methionine + cytosine38 in tRNA ⇌ {\displaystyle \rightleftharpoons } S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA The eukaryotic enzyme catalyses methylation of cytosine38 in the anti-codon loop of tRNAAsp(GTC), tRNAVal(AAC) and tRNAGly(GCC). (wikipedia.org)
Cytosine C5 methylation is an important epigenetic control mechanism in a wide array of eukaryotic organisms and generally carried out by proteins of the C-5 DNA methyltransferase family (DNMTs). (mdpi.com)
Trm10 catalyzes the methylation of guanosine residues at position 9 of several tRNA species, a modification that is conserved throughout eukaryotic tRNAs (Jackman et al. (osu.edu)
MRPP2 also moonlights in a complex with the MRPP1 (also known as TRMT10C) protein for N1-methylation of purines at position 9 of mitochondrial tRNA, and in a complex with MRPP1 and MRPP3 (also known as PRORP) proteins for 5'-end processing of mitochondrial precursor tRNA. (ox.ac.uk)
Methylation is deposited by a multicomponent methyltransferase complex ("writers") consisting of a core writer complex and an interacting complex. (encyclopedia.pub)
Crystal Structure of the Human tRNA m(1)A58 Methyltransferase-tRNA(3)(Lys) Complex: Refolding of Substrate tRNA Allows Access to the Methylation Target. (ucsf.edu)
Enzyme10
TRNA (cytosine38-C5)-methyltransferase (EC 2.1.1.204, hDNMT2 (gene), DNMT2 (gene), TRDMT1 (gene)) is an enzyme with the systematic name S-adenosyl-L-methionine:tRNA (cytosine38-C5)-methyltransferase. (wikipedia.org)
While the DNMT2 protein contains all the catalytic motifs of a canonical DNA methyltransferase, we have shown that the enzyme is a multisubstrate tRNA methyltransferase that methylates certain tRNAs. (dkfz.de)
Here, we show that Nsp5 binds and cleaves human tRNA methyltransferase 1 (TRMT1), a host enzyme required for a prevalent post-transcriptional modification in tRNAs. (elifesciences.org)
This manuscript provides important insights into the degradation of a host tRNA modification enzyme TRMT1 by SARS-CoV-2 protease nsp5. (elifesciences.org)
2022. Pronounced sequence specificity of the TET enzyme catalytic domain guides its cellular function . (cardiff.ac.uk)
The formation of Me-CpG is catalyzed by the enzyme DNA methyltransferase . (explained.today)
As a post-doctoral fellow in the laboratory of Eric Phizicky at the University of Rochester School of Medicine, she participated in identification of several previously undiscovered tRNA modification enzymes, including several that exhibit novel enzyme chemistry. (osu.edu)
The Jackman laboratory utilizes the principles and techniques of mechanistic enzymology and enzyme kinetics to elucidate the molecular mechanisms of tRNA processing enzymes. (osu.edu)
These same principles of mechanistic enzymology, in combination with the powerful approaches of yeast genetics and molecular biology, also allow the lab to investigate the biological function of enzymes in living cells, including a tRNA splicing enzyme and a tRNA methyltransferase whose biological roles in the yeast Saccharomyces cerevisiae are well-defined, but whose possible role(s) in other organisms remain a mystery. (osu.edu)
2003). Trm10, like Thg1, is a member of a previously undescribed family of proteins with no identifiable homology to any other enzyme family, including other S-adenosyl-methionine (SAM)-dependent methyltransferases. (osu.edu)
tRNA synthetase class II core domain (G, Seryl-tRNA synthetase N-terminal domain [Interproscan]. (ntu.edu.sg)
Subunit1
GCD14 is a subunit of the tRNA methyltransferase complex and is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA [1]. (gzsys.org.cn)
Certain tRNAs1
The methyltransferase 1-WD repeat-containing protein 4 (METTL1-WDR4) complex is the methyltransferase that modifies G46 in the variable loop of certain tRNAs, and its dysregulation drives tumorigenesis in numerous cancer types8-14. (bvsalud.org)
MRNA3
SARS-CoV-2 nsp14 guanine-N7-methyltransferase plays an important role in the viral RNA translation process by catalyzing the transfer of a methyl group from S-adenosyl-methionine (SAM) to viral mRNA cap. (bvsalud.org)
Particularly, Lsm12 is possibly involved in mRNA degradationor tRNA splicing, and Lsm13-16 in the regulation of the mitotic G2/Mphase. (embl-heidelberg.de)
In addition, RNA modifications have been characterized not only in abundant non-coding RNA, such as transfer RNA (tRNA), ribosomal RNA (rRNA), and small nuclear RNA (snRNAs), but also in messenger RNA (mRNA) [ 2 ] . (encyclopedia.pub)
RRNA2
Furthermore, instead of 5S rRNA, structurally similar mtDNA-encoded tRNAs occupy an equivalent region in the central protuberance of the mtLSU. (nature.com)
Depending on the organism and availability, mt-tRNA Val or mt-tRNA Phe are incorporated, most likely due to their genomic proximity to mt-rRNA genes and consequent near stoichiometric presence of their transcripts 4 , 8 . (nature.com)
Protein9
Mg2+ is required for the catalytic activity of TrmD, a bacteria-specific methyltransferase that is made up of a protein topological knot-fold, to synthesize methylated m1G37-tRNA to support life. (jefferson.edu)
Human cells infected with SARS-CoV-2 exhibit a decrease in TRMT1 protein levels and TRMT1-catalyzed tRNA modifications, consistent with TRMT1 cleavage and inactivation by Nsp5. (elifesciences.org)
In a thermophilic bacteria, activated sulfur which bond to a sulfur-carrier protein TtuB [Fig. 2] is transferred to tRNA by a sulfur transferase. (go.jp)
42520) hypothetical protein CP001857 tRNA Arcpr_R0002 complement(42639. (go.jp)
Novel patient missense mutations in the HSD17B10 gene affect dehydrogenase and mitochondrial tRNA modification functions of the encoded protein. (ox.ac.uk)
MRPP2 (also known as HSD10/SDR5C1) is a multifunctional protein that harbours both catalytic and non-catalytic functions. (ox.ac.uk)
Both mutant proteins showed significant reduction in the dehydrogenase, methyltransferase and tRNA processing activities compared to wildtype, associated with reduced stability for protein with p.V12L, whereas the protein carrying p.V176M showed impaired kinetics and complex formation. (ox.ac.uk)
The interacting complex contains the RNA binding motif protein 15/15B (RBM15/15B), vir-like m6A methyltransferase associated (VIRMA), zinc finger CCCH-type containing 13 (ZC3H13), and HAKAI which support the functioning and positioning of a writer complex. (encyclopedia.pub)
The TRMT1 cleavage fragments exhibit altered RNA binding activity and are unable to rescue tRNA modification in TRMT1-deficient human cells. (elifesciences.org)
These findings provide evidence that Nsp5-dependent cleavage of TRMT1 and perturbation of tRNA modification patterns contribute to the cellular pathogenesis of SARS-CoV-2 infection. (elifesciences.org)
We report that RNA modification of the cap portion by host 2'-O-ribose methyltransferase 1 (MTr1) is essential for the initiation of influenza A and B virus replication, but not for other cap-snatching viruses. (bvsalud.org)
The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA. (gzsys.org.cn)
Initial projects in the laboratory will focus on several recently-discovered tRNA modification enzymes. (osu.edu)
Adenine1
TRM61 tRNA (adenine(57)-N(1)/adenine(58)-N(1) or adenine(58)-N(1))-MT (EC 2.1.1.219 or EC 2.1.1.220 ). (expasy.org)
Gcd141
We uncovered the functions of ABCE proteins Rli1/ABCE1 and Arb1 in PIC assembly and ribosome biogenesis, and identified the tRNA methyltransferase Gcd10/Gcd14, which contributed to the discovery of the TRAMP-mediated RNA surveillance pathway. (nih.gov)
Complex3
It causes been that the download insurgency complex peptide 2( SH2 tRNA) of PLC-gamma and of cyanobacterial shedding features( such as elongating site and necrosis pleiotropic circulation) develop their presence toward post-translational p38 Humans of the FGFR. (erik-mill.de)
In the core complex, methyltransferase-like 3 (METTL3) is the catalytic component, methyltransferase-like 14 (METTL14) liaises with METTL3 to recognize the substrate, and WTAP guides METTL3/14 heterodimer [ 7 ] . (encyclopedia.pub)
Non-catalytic component of the multisynthase complex. (epigentek.com)
Modifications2
These results will be of interest to virologists interested in studying the alterations in tRNA modifications, host methyltransferases, and viral infections. (elifesciences.org)
Among them, sulfur modifications of tRNA are important for tRNA functions, such as codon recognition and stabilization of the tertiary structure [Fig. 1]. (go.jp)
Cytoplasmic1
Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. (epigentek.com)
Mechanism4
However, neither the location of Mg2+ in the structure of TrmD nor its role in the catalytic mechanism is known. (jefferson.edu)
Structural Basis for the Catalytic Mechanism of DncV, Bacterial Homolog of Cyclic GMP-AMP Synthase. (nurekilab.net)
We identified the eIF2α phosphatases in yeast and made key contributions to elucidating the mechanism whereby phosphorylated eIF2 inhibits its GDP-GTP exchange factor, eIF2B, defining the catalytic and regulatory subcomplexes of eIF2B and their distinct roles in binding phosphorylated or nonphosphorylated eIF2. (nih.gov)
She received her Ph.D. degree in Biochemistry from Duke University in Durham, NC, working with Carol Fierke and Chris Raetz to investigate the catalytic mechanism of a metal-dependent metalloamidase (LpxC), a target for novel anti-microbial compounds that catalyzes the committed step during biosynthesis of the lipid A component of lipopolysaccharide. (osu.edu)
Molecular1
Our results provide a molecular understanding of tRNA substrate recognition and phosphorylation-mediated regulation of METTL1-WDR4, and reveal the presumed disordered N-terminal region of METTL1 as a nexus of methyltransferase activity. (bvsalud.org)
Cytosine1
DNA (cytosine-5-)-MT (EC 2.1.1.37 ) and tRNA (cytosine(38)-C(5))-MT (EC 2.1.1.204 ). (expasy.org)
Purification of individual SARS-CoV-2 proteins from human cells have identified a potential interaction between a catalytic-inactive version of Nsp5 with human tRNA methyltransferase 1 (TRMT1) ( Gordon et al , 2020b ). (elifesciences.org)
m 6 A is catalysed by RNA methyltransferases METTL3, METTL14 and WTAP (writers), m 6 A is removed by the demethylases FTO and ALKBH5 (erasers) and interacts with m6A-binding proteins, such as YT521-B homology (YTH) domain-containing proteins. (biomedcentral.com)
Activity6
The computational results are validated by experimental mutation studies, which demonstrate the importance of the Mg2+-binding residues for the catalytic activity. (jefferson.edu)
Unexpectedly, we find that the predicted disordered N-terminal region of METTL1 is part of the catalytic pocket and essential for methyltransferase activity. (bvsalud.org)
Furthermore, we reveal that S27 phosphorylation in the METTL1 N-terminal region inhibits methyltransferase activity by locally disrupting the catalytic centre. (bvsalud.org)
We report a structure-guided design and synthesis of 3-(adenosylthio)benzoic acid derivatives as nsp14 methyltransferase inhibitors resulting in compound 5p with subnanomolar inhibitory activity and improved cell membrane permeability in comparison with the parent inhibitor. (bvsalud.org)
However, mitoribosome biogenesis does not depend on the methyltransferase activity of MRM2. (nature.com)
We also made key contributions to identifying the eIF2α kinase Gcn2 and elucidating its regulation by amino acids, via allosteric control of kinase activity by uncharged tRNAs and Gcn2 regulatory domains, and by the TOR pathway through Gcn2-Ser577 phosphorylation. (nih.gov)
Cellular1
Here we show, through structural, biochemical and cellular studies of human METTL1-WDR4, that WDR4 serves as a scaffold for METTL1 and the tRNA T-arm. (bvsalud.org)
Recognition1
The identification of an additionalmethyltransferase domain at the C-terminus of one of the Lsm12 proteinsalso led to the recognition of three new groups of methyltransferases,presumably dependent on S-adenosyl-l-methionine. (embl-heidelberg.de)
Zinc1
Further computationalanalyses revealed that some methyltransferases contain putativeRNA-binding helix-turn-helix domains and zinc fingers. (embl-heidelberg.de)
Enzymes1
This process, catalyzed by enzymes such as caffeoyl-CoA O-methyltransferase , is a key reaction in the biosynthesis of lignols , percursors to lignin , a major structural component of plants. (explained.today)
Show1
Our findings show crosstalk between methyltransferase inhibition and TGFß-dependent activation of a remote enhancer to reverse CDKN2A/B silencing. (bvsalud.org)