AMPKSerinePhosphataseRegulatory subunitGamma subunitsGeneSubfamilySuperfamilyPhosphorylationHuman recombinantInhibitorPhosphoglycerate kinaseAmino acidsGENESEnzymeReceptorPRKACAPolypeptideIsoformsPhosphorylatesELISA KitPyruvate dehydrogenaseHoloenzymePathwayAntibodyVitroMeSHNuclearRegulationFamilyMutationsDomainCytoskeletalActivityLigandsInteractions
AMPK21
- 3. Littler DR, Walker JR, Davis T, Wybenga-Groot LE, Finerty PJ, Newman E, Mackenzie F, Dhe-Paganon S. (2010) A conserved mechanism of autoinhibition for the AMPK kinase domain: ATP-binding site and catalytic loop refolding as a means of regulation. (guidetopharmacology.org)
- AMPK subfamily: protein kinase AMP-activated catalytic subunit alpha 2. (guidetopharmacology.org)
- PRKAA1 (Protein kinase AMP-activated catalytic subunit alpha 1), also known as AMPK α1, is an energy sensor that plays a key role in regulating cell energy metabolism. (bocsci.com)
- AMPK α1 is a catalytic subunit of heterotrimeric AMPK protein with a length of 548 amino acids. (bocsci.com)
- AMPK proteins are dysfunctional in several human diseases, including diabetes and metabolic syndrome, cardiovascular disease, neurodegenerative diseases and many types of cancer. (bocsci.com)
- It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). (wikipedia.org)
- The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. (wikipedia.org)
- 5'-AMP-activated protein kinase subunit beta-2, AMPK subunit beta-2. (prospecbio.com)
- PRKAB2 is a regulatory subunit of the AMP-activated protein kinase (AMPK). (prospecbio.com)
- AMPK is a heterotrimer contains an alpha catalytic subunit and non-catalytic beta and gamma subunits. (prospecbio.com)
- AMP-activated protein kinase (AMPK) is involved in the regulation of cellular energy homeostasis and, on the whole animal level, in regulating energy balance and food intake. (usda.gov)
- The AMPK enzyme complex consists of one catalytic (alpha) and two regulatory (beta and gamma) subunits. (usda.gov)
- AMP-activated protein kinase (AMPK) is a master metabolic regulator for controlling cellular energy homeostasis. (rcsb.org)
- cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. (thermofisher.com)
- The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. (thermofisher.com)
- Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. (thermofisher.com)
- PRKACB is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK. (thermofisher.com)
- Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. (nih.gov)
- In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. (nih.gov)
- The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). (ageing-map.org)
- This subunit is one of the gamma regulatory subunits of AMPK. (ageing-map.org)
Serine6
- The protein encoded by this gene belongs to the serine/threonine protein kinase family. (wikipedia.org)
- Here, we report that during intestinal inflammation, multiple inflammatory cytokines promote the activity of a master regulator of cell proliferation and apoptosis, serine/threonine kinase CK2. (diva-portal.org)
- A member of the family of PI3Ks/mTOR is a physiologically stable serine/threonine protein kinase [ 11 ]. (biomedcentral.com)
- Casein Kinase 2 Alpha 1 (CKII) is a serine/threonine protein kinase with structural activity. (biomedcentral.com)
- A protein serine-threonine kinase that catalyzes the PHOSPHORYLATION of I KAPPA B PROTEINS. (bvsalud.org)
- All NSP proteins contain an NH 2 -terminal SH2 (Src homology domain 2) domain, a central proline/serine-rich domain, and a COOH-terminal domain with modest homology to Ras subfamily GDP-exchange factors (GEFs). (molvis.org)
Phosphatase3
- Here DARPP-32 phosphorylation by protein kinase A (PKA), DARPP-32 into a powerful protein phosphatase 1 (PP1) inhibitor. (pp1a.com)
- This study found an increase in excess GR transcriptional activity of protein phosphatase 1 alpha (PP1α) in HEK-293 cells and a decrease in the expression levels of GR-responsive gene knockdown following PP1α model A549 cells endogenously . (pp1a.com)
- The BCKD complex, which is associated with the inner mitochondrial membrane, has 3 different catalytic components (ie, E1, E2, E3) and 2 associated regulatory enzymes (ie, BCKD phosphatase, BCKD kinase). (medscape.com)
Regulatory subunit2
- This enzyme also activates the transcription factor NF-KAPPA B and is composed of alpha and beta catalytic subunits, which are protein kinases and gamma, a regulatory subunit. (bvsalud.org)
- Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. (antibodies-online.com)
Gamma subunits2
- 1996). "Characterization of AMP-activated protein kinase beta and gamma subunits. (wikipedia.org)
- Previously, we found that chickens expressed three gamma subunits (gamma 1-3), each encoded by a different gene. (usda.gov)
Gene10
- A single nucleotide polymorphism (SNP) of the protein kinase catalytic subunit alpha-1 gene ( PRKAA1 ) that confers susceptibility to gastric cancer (GC) was identified by genome-wide association in several case-control studies. (oncotarget.com)
- 5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene. (wikipedia.org)
- The presence or absence of one exon by alternative splicing of the gamma-1 gene resulted in two transcript variants which code for predicted proteins containing 298 amino acids (4 cystathionine beta-synthase domains, CBSD) or 276 amino acids (3 CBSD). (usda.gov)
- For the gamma-2 gene, use of an alternate initiation site and promoter and/or alternative splicing resulted in four different transcript variants which code for predicted proteins containing 567 (4 CBSD), 452 (4 CBSD), 328 (3 CBSD) and 158 (2 CBSD) amino acids. (usda.gov)
- Alternative splicing of exon 3 in the gamma-3 gene resulted in a shift of the open reading frame and the production of 'long' and 'short' transcript variants that code for predicted proteins of 383 and 363 amino acids, both with 4 CBSD. (usda.gov)
- What does this gene/protein do? (cancerindex.org)
- Description of the protein which includes the UniProt Function and the NCBI Gene Summary. (nih.gov)
- Introduction: Alterations of the cAMP signaling pathway are described in adrenal tumors causing Cushing syndrome, specifically mutations in the gene coding for the protein kinase A (PKA) catalytic subunit alpha (PRAKCA) in cortisol producing adenomas (CPA) with overt Cushing syndrome.Materiel and Methods: Eight CPAs without PRKACA mutations were analyzed by whole exome sequencing. (endocrine-abstracts.org)
- This gene encodes one of the regulatory subunits. (nih.gov)
- The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. (antibodies-online.com)
Subfamily1
- also alpha -PAK and p65-PAK) is both a cytoplasmic and nuclear 58-60 kDa member of PAK group I, STE20 subfamily, STE Ser/Thr protein kinase family of molecules. (rndsystems.com)
Superfamily2
- The MDR superfamily with ~350-residue subunits contains the classical liver alcohol dehydrogenase (ADH), quinone reductase, leukotriene B4 dehydrogenase and many more forms. (researchgate.net)
- Belongs to the protein kinase superfamily. (lu.se)
Phosphorylation6
- Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. (rcsb.org)
- Intersecting analysis, molecular docking, and pathway validation analysis showed that risperidone influences the adipocytokine signaling pathway by targeting MAPK14 (mitogen-activated protein kinase 14), MAPK8 (mitogen-activated protein kinase 8), and RXRA (retinoic acid receptor RXR-alpha), thereby inhibiting long-chain fatty acid β-oxidation by decreasing STAT3 (signal transducer and activator of transcription 3) expression and phosphorylation. (frontiersin.org)
- DARPP-32 can itself inhibit the following PKA DARPP-32 phosphorylation by cyclin-dependent kinase 5 (Cdk5). (pp1a.com)
- Mechanistic investigations revealed reduce GR-Ser211 phosphorylation following PP1α silencing and provide the first indication of the involvement of glycogen synthase kinase 3 (GSK-3). (pp1a.com)
- EC 1.1.1.34 is inactivated by the phosphorylation of the enzyme protein. (expasy.org)
- This phosphorylation is regulated by the ataxia telangiectasia mutated (ATM) and the Rad3-related (ATR) kinases in response to various genotoxic stresses, making it a critical regulatory element of DNA-PKcs ( 6 , 10 ). (amegroups.org)
Human recombinant1
- A screen profiling 158 kinase inhibitors (Calbiochem Protein Kinase Inhibitor Library I and II, catalogue numbers 539744 and 539745) for their inhibitory activity at 1µM and 10µM against 234 human recombinant kinases using the EMD Millipore KinaseProfiler TM service. (guidetopharmacology.org)
Inhibitor1
- Inhibition of DNA-PKcs activity via a small interfering RNA or a kinase inhibitor results in mitosis delay, abnormal spindle formation, and chromosome misalignment. (amegroups.org)
Phosphoglycerate kinase1
- Disruption and effective anti-GBM agents were triggered in the hypoxic region, leading to efficient tumor suppression by using phosphoglycerate kinase 1 (PGK1) silencing to enhance paclitaxel-induced chemotherapy and sensitize hypoxic GBM cells to ionizing radiation. (bvsalud.org)
Amino acids2
- A synthesized peptide derived from human PKA alpha/beta CAT, corresponding to a region within N-terminal amino acids. (thermofisher.com)
- In the following years, Dancis et al identified the pathogenetic compounds as branched-chain amino acids and their corresponding alpha-keto acids. (medscape.com)
GENES4
- Genes that suppress carcinogenesis have been classified as gatekeepers that regulate cellular proliferation and cell death and as caretakers that are primarily encode DNA repair proteins required for the maintenance of genome integrity ( 15 ). (amegroups.org)
- This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. (nih.gov)
- Transcription factor that binds to the interleukin-6 (il-6)-responsive elements identified in the promoters of various acute-phase protein genes. (lu.se)
- Whole exome sequencing of patients who experienced severe influenza reveals several genes, including scaffold protein AHNAK, with predicted loss-of-function variants that are also identified in our proteomic analyses. (cdc.gov)
Enzyme3
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Protein Kinase C Alpha (PKCa) in serum, plasma, tissue homogenates and other biological fluids. (scalegen.com)
- Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Protein Kinase C Alpha (PKCa) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. (scalegen.com)
- Description: Enzyme-linked immunosorbent assay kit for quantification of Human 5'-AMP-activated protein kinase catalytic subunit alpha-2 in samples from serum, plasma, tissue homogenates and other biological fluids. (bionotatki.com)
Receptor3
- DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a key component of the non-homologous end-joining (NHEJ) pathway, is involved in DNA double-strand break repair, immunocompetence, genomic integrity, and epidermal growth factor receptor signaling. (amegroups.org)
- Sorafenib (Sor) can block receptor tyrosine kinases, which are involved in the development of new blood vessels, as well as cell proliferation as a result [ 22 ]. (biomedcentral.com)
- 1984) 'Protein Kinase Activity in the Rat Mammary Gland during Pregnancy, Lactation, and Weaning: A Correlation with Growth but not with Progesterone Receptor Levels *', Endocrinology, 115(5), pp. 1918-1924. (bomiprot.org)
PRKACA1
- maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. (rcsb.org)
Polypeptide1
- Microtubules are composed of heterodimeric subunits containing an alpha - and beta -tubulin polypeptide. (rndsystems.com)
Isoforms1
- 1. Gamm, D. M., Baude, E. J. and Uhler, M. D. (1996) 'The Major Catalytic Subunit Isoforms of cAMP-dependent Protein Kinase Have Distinct Biochemical Properties in Vitro and in Vivo', Journal of Biological Chemistry, 271(26), pp. 15736-15742. (bomiprot.org)
Phosphorylates1
- In response to amino acid deprivation or UV irradiation, GCN2 phosphorylates the alpha subunit of eukaryotic initiation factor 2 (eIF2a) at Ser51 and induces a delay in entry to S phase of the cell cycle. (rndsystems.com)
ELISA Kit1
- Description: A sandwich ELISA kit for detection of Protein Kinase C Alpha from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (scalegen.com)
Pyruvate dehydrogenase1
- The E3 component is associated with 2 additional alpha-ketoacid dehydrogenase complexes, namely pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase. (medscape.com)
Holoenzyme1
- cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. (thermofisher.com)
Pathway1
- The catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is the key component of the non-homologous end-joining (NHEJ) pathway for DNA double-strand break (DSB) repair and is required for cellular resistance to ionizing radiation (IR) ( 1 , 2 ). (amegroups.org)
Antibody1
- The following product was used in this experiment: PKA C alpha/beta Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-115763, RRID AB_2900398. (thermofisher.com)
Vitro1
- The in vitro treatment of intestinal epithelial cell lines with cytokines resulted in increased CK2 expression and nuclear translocation of its catalytic alpha subunit. (diva-portal.org)
MeSH1
- I-kappa B Kinase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (uchicago.edu)
Nuclear1
Regulation1
- Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle. (ageing-map.org)
Family1
- Tyr protein kinase family. (lu.se)
Mutations2
- Scope includes mutations and abnormal protein expression. (cancerindex.org)
- Mutations in E3 cause additional deficiencies of pyruvate and alpha-ketoglutarate dehydrogenases. (medscape.com)
Domain3
- It contains one GTPase binding domain (aa 75-105) plus a protein kinase catalytic region (aa 250-521). (rndsystems.com)
- GCN2 contains an RWD domain (aa 21-134), a pseudo-kinase domain (aa 296-539), a catalytic domain (aa 590-1001), and a histidyl tRNA synthase-like domain (aa 1022-1493) followed by a C-terminal ribosome interacting region. (rndsystems.com)
- Alternate splicing generates an isoform with a short deletion in the kinase domain and an isoform that is truncated at the N-terminal end of the kinase domain. (rndsystems.com)
Cytoskeletal1
Activity3
- A screen profiling the inhibitory activity of 178 commercially available kinase inhibitors at 0.5µM against a panel of 300 recombinant protein kinases using the Reaction Biology Corporation Kinase Hotspot SM platform. (guidetopharmacology.org)
- 2013) A broad activity screen in support of a chemogenomic map for kinase signalling research and drug discovery. (guidetopharmacology.org)
- Enhanced mucosal CK2 protein expression and activity were observed in animal models of chronic colitis, particularly within intestinal epithelial cells (IECs). (diva-portal.org)
Ligands1
- Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. (cancerindex.org)
Interactions1
- Using affinity purification-mass spectrometry and global phosphoproteomic and protein abundance analyses using three IAV strains (pH1N1, H3N2, H5N1) in three human cell types (A549, NHBE, THP-1), we map 332 IAV-human protein-protein interactions and identify 13 IAV-modulated kinases. (cdc.gov)