CatalysisEnzymesBiocatalysisCatalytic DomainKineticsModels, MolecularProtein ConformationModels, ChemicalBinding SitesHydrogen-Ion ConcentrationSubstrate SpecificityMolecular Sequence DataCrystallography, X-RayMutagenesis, Site-DirectedAmino Acid SequenceEscherichia coliRaynaud DiseaseRNA, CatalyticHydrolysisProtein BindingBase SequenceMutationMolecular StructureProtein Structure, TertiaryHistidineRecombinant ProteinsStructure-Activity RelationshipOxidation-ReductionStereoisomerismSequence Homology, Amino AcidAntibodies, CatalyticHydrogen BondingAmino Acid SubstitutionThermodynamicsEnzyme StabilityAspartic AcidProtein Structure, SecondaryMetalsSequence AlignmentMagnesiumBacterial ProteinsModels, BiologicalTemperatureMolecular ConformationAdenosine TriphosphateProtonsNo-Reflow PhenomenonCysteineCloning, MolecularTime FactorsConserved SequenceSpectrophotometry