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  • Proteins
  • View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate , the pathway glutathione biosynthesis and in Sulfur metabolism . (uniprot.org)
  • To explore the properties of multi-domain BjCHI1, we have expressed recombinant BjCHI1 and two derivatives, which lack one (BjCHI2) or both (BjCHI3) chitin-binding domains, as secreted proteins in Pichia pastoris. (deepdyve.com)
  • Unfortunately due to the conformation-dependent nature of ligand binding, the act of discovering a new small molecule that will bind these particular proteins is analogous to aiming at a moving target. (illinois.edu)
  • Within the nucleus, Nrf2 is believed to exert its transcriptional function by forming heterodimers with small Maf (v-maf musculoaponeurotic fibrosarcoma oncogene family) proteins, binding to ARE-containing promoters, and recruiting transcription coactivators to help remodel chromatin structures and facilitate the formation of basal transcription machinery ( 22 , 29 , 30 , 41 , 50 , 74 ). (pubmedcentralcanada.ca)
  • enzymes
  • MMPs are secreted or membrane bound enzymes that degrade numerous substrates. (bachem.com)
  • Glutamate dehydrogenases ( EC:1.4.1.2 , EC:1.4.1.3 , and EC:1.4.1.4 ) (GluDH) are enzymes that catalyse the NAD- and/or NADP-dependent reversible deamination of L-glutamate into alpha-ketoglutarate [ PMID: 1358610 , PMID: 8315654 ]. (ebi.ac.uk)
  • Whalen, K.L., Spies, M.A. Flooding enzymes: quantifying the contributions of interstitial water and cavity shape to ligand binding using extended linear response free energy calculations. (uiowa.edu)
  • Many of these flexible enzymes are of biological interest, and hindering their function via binding of competitive inhibitors would open up valuable therapeutic avenues. (illinois.edu)
  • ligand
  • First and foremost, the Spies lab is interested in structure-based computer-aided drug discovery, and the discovery of glutamate racemase inhibitors is a project located at the intersection of ligand binding and catalysis, where small molecules inhibit the catalytic process. (illinois.edu)
  • Finally, my third project involves more fundamental biochemistry in that we closely examine facets of molecular recognition such as conformational changes induced by ligand binding, and the role of interstitial water. (illinois.edu)
  • The R324C mutation also leads to a loss of the direct interaction with ADP, resulting in a higher mobility of the ligand in the binding pocket. (biomedcentral.com)
  • carboxamide ligand substitutions in the zinc binding site of carbonic anhydrase II alter metal coordination geometry but retain catalytic activity. (proteopedia.org)
  • cDNA
  • Mowbray, Sherry 2004-12-30 00:00:00 We previously isolated a Brassica juncea cDNA encoding BjCHI1, a novel chitinase with two chitin-binding domains. (deepdyve.com)
  • activity
  • a href='/help/catalytic_activity' target='_top'>More. (uniprot.org)
  • Relative to CHN A, a variant with two spacers in tandem, which binds chitin, showed very low hydrolytic activity towards chitin and Nod factors, but comparable lysozyme activity and enhanced antifungal activity. (deepdyve.com)
  • They contain a Gly-rich region containing a conserved Lys residue, which has been implicated in the catalytic activity, in each case a reversible oxidative deamination reaction. (ebi.ac.uk)
  • Using our structure and assay, we identify an LPLAT thioesterase activity, and we provide experimental evidence to support an ordered-binding and "reset" mechanistic model for LpxM function. (pnas.org)
  • CATALYTIC ACTIVITY: L-glutamate = 4-aminobutanoate + CO(2). (genome.jp)
  • ACTIVITY REGULATION: Up-regulated by calmodulin binding at CC physiological pH. (genome.jp)
  • This is demonstrated by catalytic activity measurements for ester hydrolysis and CO2 hydration, as well as sulfonamide inhibitor affinity assays. (proteopedia.org)
  • Nrf2
  • These findings demonstrated that acetylation of Nrf2 by p300/CBP augments promoter-specific DNA binding of Nrf2 and established acetylation as a novel regulatory mechanism that functions in concert with Keap1-mediated ubiquitination in modulating the Nrf2-dependent antioxidant response. (pubmedcentralcanada.ca)
  • amino
  • Here, we constructed a panel of APOBEC3G amino acid substitution mutants and performed a series of biochemical, genetic, and structural assays to distinguish between "Brim" and "Kink" models for single-strand DNA binding. (mdpi.com)
  • substitutions
  • In support of the Brim model, arginine to glutamate substitutions at positions 213, 215, and 320 also compromised these APOBEC3G activities. (mdpi.com)
  • membrane
  • Pyroglutamyl-peptidase II and aminopeptidase N are membrane-bound with N-terminal cytoplasmic and transmembrane domains. (ebi.ac.uk)
  • The insect aminopeptidase N ( M01.013 ) is membrane-bound by a glycosylphosphatidinylinositide anchor. (ebi.ac.uk)
  • While most [NiFe] hydrogenases are inactivated by O 2 , a small subgroup, including the membrane-bound [NiFe] hydrogenase (MBH) of Ralstonia eutropha , is able to overcome aerobic inactivation by catalytic reduction of O 2 to water. (pnas.org)
  • Biosynthesis
  • The calmodulin-binding CC is calcium-dependent and it is proposed that this may, directly or CC indirectly, form a calcium regulated control of GABA biosynthesis. (genome.jp)
  • Structures
  • These structures are the first structures for any eukaryotic GGT that include a molecule in the active site covalently bound to the catalytic Thr-381. (osti.gov)
  • In contrast, the atomic structures and catalytic mechanisms of the fungal ( 6 ) and mammalian (A. Changela, C.K.H., A. Martins, S.S., and A. Mondragon, unpublished observations) RNA triphosphatases are completely different. (pnas.org)
  • molecule
  • Metal geometry changes to trigonal bipyramidal in H119N CAII due to the addition of a second water molecule to the zinc coordination polyhedron and also in H94N CAII due to the displacement of zinc-bound hydroxide by the bidentate coordination of a Tris molecule. (proteopedia.org)