Second, it has fairly high capture capability and catalytic activity because it is presented as aggregates of abundant antibodies and enzymes. (cdc.gov)
Enzymes of various catalytic functions in Streptomyces are useful as members of an artificial gene cluster constructed in Escherichia coli for fermentative production of plant-specific flavonoids, including isoflavones and unnatural compounds. (go.jp)
The naturally designed architecture, semi-permeability, and catalytic improvement of carboxysomes have inspired rational design and engineering of new nanomaterials to incorporate desired enzymes into the protein shell for enhanced catalytic performance. (nature.com)
The study provides insights into the assembly and formation of carboxysomes and paves the way for engineering carboxysome shell-based nanoreactors to recruit specific enzymes for diverse catalytic reactions. (nature.com)
Among these hydrogenases, [FeFe]-hydrogenases are the most efficient enzymes for catalytic hydrogen turnover, and arguably the promising biocatalysts for hydrogen production. (nature.com)
This represents a step towards synthetically engineering large and stable nanoreactors that are capable of sequestering high copy numbers of cargo enzymes and providing selective permeability to substrates to boost enzyme activities. (nature.com)
7: Zanaroli G, Negroni A, Calisti C, Ruzzi M, Fava F. Selection of commercial hydrolytic enzymes with potential antifouling activity in marine environments Enzyme Microb Technol. (moleculardepot.com)
In the human cell, antioxidant molecules include both nonenzymatic compounds such as glutathione (GSH), thioredoxin (Trx), ascorbate, α -tocopherol, β -carotene, and coenzyme Q10 and enzymes such as catalase, glutathione peroxidase, and superoxide dismutase (SOD) [ 8 ]. (hindawi.com)
Proteins2
(1) The tyramide functionality enables high density biotin labeling of nucleic acid sequences or proteins through the catalytic activity of horseradish peroxidase. (biosearchtech.com)
Complex patterns of posttranslational modifications of chromatin including acetylation of histones establish combinatorial signals that are apparently interpreted by chromatin-binding proteins and the gene expression machinery to control transcriptional activity of individual genes. (aacrjournals.org)
Protein4
Because the synthetic method requires no organic solvent and because of the distinct hierarchical nanostructure, protein-inorganic nanoflowers display enhanced catalytic activity and stability and would be a promising tool in biocatalytical processes and biological and biomedical fields. (cdc.gov)
It comprises the chitin-binding protein ChbB in fusion with a His-tag as well as with a Strep-tag, the latter of which was chemically coupled to horseradish peroxidase. (mdpi.com)
Like tyramide signal amplification (TSA), PSA™ also uses the catalytic activity of horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid sequence in situ. (aatbio.com)
It contains one GTPase binding domain (aa 75-105) plus a protein kinase catalytic region (aa 250-521). (rndsystems.com)
Inhibition4
Inhibition from the catalytic activity of purified PTK6 by chosen kinase inhibitors IC50 (nM)akinase assay. (scienceexhibitions.org)
MMP catalytic activity is regulated at several levels, including transcription, as well as activation and inhibition. (molcells.org)
In this article, we describe the development and validation of a novel method to identify hBChE variants with OP-resistant functional activity (decreased rate of OP inhibition). (aspetjournals.org)
SB216763-treated HLE-B3 cells caused marked inhibition of GSK-3β activity prompting a significant increase in the translocation of cytoplasmic β-catenin to the nucleus. (molvis.org)
Tumor2
Increased GCN2 activity in solid tumors enhances the production of amino acids and contributes to tumor cell survival in conditions of nutrient deprivation. (rndsystems.com)
Therefore, targeting drugs molecules in the PTEN signaling pathway may be another strategy to suppress tumor cells by inhibiting tumor activity [ 7 , 8 ]. (aging-us.com)
Oxidative1
In a similar manner, EDTA is added to some food as a preservative or stabiliser to prevent catalytic oxidative decolouration, which is catalysed by metal ions. (wikipedia.org)
Assay3
In addition, the assay allows rapid scoring of the activity of chitin-synthases. (mdpi.com)
Steady-state kinetic constants using oxalate as the substrate determined by multiple injection ITC are comparable to those obtained by a continuous spectrophotometric assay in which H2O2 production is coupled to the horseradish peroxidase-catalyzed oxidation of 2,2′-azinobis-(3-ethylbenzthiazoline-6- sulfonic acid) and by membrane inlet mass spectrometry. (kennesaw.edu)
To this end, a horseradish peroxidase-catalyzed spectrophotometric method was used as an assay for H(2)O(2). (unl.pt)
Hydrogen6
In particular, peroxidase, which oxidizes transparent colorimetric substrates to become a colored product in the presence of hydrogen peroxide, is the most common enzyme that is used in colorimetric bioassays. (k-erc.eu)
for example, peroxidase-mimicking nanozymes demonstrate oxidase-like activity that oxidizes colorimetric substrates in the absence of hydrogen peroxide, which keeps them away from precisely detecting the target materials, such as hydrogen peroxide. (k-erc.eu)
Professor Jinwoo Lee from the Department of Chemical and Biomolecular Engineering and his team were able to synthesize a peroxidase-mimicking nanozyme with superior catalytic activity and selectivity toward hydrogen peroxide. (k-erc.eu)
A novel and efficient synthesis of highly oxidized lignans by a methyltrioxorhenium/hydrogen peroxide catalytic system. (torvergata.it)
We report here the use of the multiple injection method of ITC to characterize the catalytic properties of oxalate oxidase (OxOx) from Ceriporiopsis subvermispora (CsOxOx), a manganese dependent enzyme that catalyzes the oxygen-dependent oxidation of oxalate to carbon dioxide in a reaction coupled with the formation of hydrogen peroxide. (kennesaw.edu)
the elevated local concentration of hydrogenases in the nanoreactor led to improved hydrogen-producing activities 11 . (nature.com)
Subunits3
PF cells were found to express regulatory (p85) and catalytic (p110α and p110β) subunits of phosphatidylinositol 3′-kinase (PI3′-kinase). (jneurosci.org)
The γ subunit is the site of the enzyme's catalytic activity while the other three subunits serve regulatory functions. (phosphorylasesignals.com)
The catalytic step that initiates formation of the ferric oxy-hydroxide mineral core in the central cavity of H-type ferritin involves rapid oxidation of ferrous ion by molecular oxygen (ferroxidase reaction) at a binuclear site (ferroxidase site) found in each of the 24 subunits. (unl.pt)
Enzymatic5
2) the nanoflower has enhanced enzymatic activity and stability to produce an amplified signal. (cdc.gov)
optimisation of adsorption and determination of enzymatic activity. (cranfield.ac.uk)
Construction and interrogation of enzyme microarrays using scanning electrochemical microscopy - optimisation of adsorption and determination of enzymatic activity. (cranfield.ac.uk)
The enzymatic activity of the immobilised enzyme on the surface was in the presence of its substrate observed to give rise to substantial positive feedback between the slide and the SECM microelectrode tip. (cranfield.ac.uk)
Contribution of the enzymatic activity was discarded by site-directed mutagenesis at the RNase catalytic site. (frontiersin.org)
Antibodies1
Horseradish peroxidase-conjugated goat-anti mouse or rabbit IgG antibodies were purchased from KOMA (Korea). (molcells.org)
Detection3
The nitrogen and boron co-doped graphene was also applied to the colorimetric detection of acetylcholine, which is an important neurotransmitter and successfully detected the acetylcholine even better than the nature peroxidase. (k-erc.eu)
Some cell-permeable fluorogenic probes developed are useful for the detection of ROS while in conjunction with horseradish peroxidase (HRP). (tmu.edu.tw)
The multiple injection ITC method of measuring OxOx activity involves continuous, real-time detection of the amount of heat generated (dQ) during catalysis, which is equal to the number of moles of product produced times the enthalpy of the reaction (ΔHapp). (kennesaw.edu)
Intracellular3
Herein, a new approach for intracellular sensing of ROS by using horseradish peroxidase-encapsulated hollow silica nanospheres (designated HRP@HSNs), with satisfactory catalytic activity, cell membrane permeability, and biocompatibility, was prepared via a microemulsion method.These HRP@HSNs, combined with selective probes or targeting ligands, could be foreseen as ROS-detecting tools in specific organelles or cell types. (tmu.edu.tw)
Despite the antimicrobial activity of macrophages, Mtb has been able to establish a series of strategies to handle the host immune machinery, interfere with, and arrest the phagosome maturation, counteract mycobactericidal molecules and ultimately survive in a hostile intracellular environment ( 8 - 10 ). (frontiersin.org)
Sperm intracellular cAMP levels depend on the activity of adenylyl cyclases, mostly SACY, though transmembrane-containing adenylyl cyclases are also present, and on the activity of cyclic nucleotide phosphodiesterases (PDE) whose role is to degrade cAMP into 5'-AMP. (plos.org)
Efficiency1
In contrast, two glyoxal oxidases form Pycnoporus cinnabarinus demonstrated the highest catalytic efficiency on glyoxylic acid [ 21 ]. (cazypedia.org)
Coli1
The three-in-one antibody (anti-E. coli O157:H7 antibody)-enzyme (horseradish peroxidase)-inorganic (Cu3(PO4)2) nanoflower has some advantages over commercial enzyme-antibody conjugates. (cdc.gov)
Substrates1
The AA5_1 members are generally known as glyoxal oxidases ( EC 1.2.3.15 ), characterized members of which typically accept a range of simple aldehydes, α-hydroxycarbonyl, and α-dicarbonyl compounds as substrates, with the highest activities observed on glyoxal, methylglyoxal and glycolaldehyde [ 1 , 14 , 18 , 19 , 20 ]. (cazypedia.org)
Enzyme activity2
Through this study, we have secured core technologies to synthesize nanozymes that have high enzyme activity along with selectivity. (k-erc.eu)
Glutamate-287 (E287) of murine mature ferrochelatase is a conserved residue in all known sequences of ferrochelatase, is present at the active site of the enzyme, as inferred from the Bacillus subtilis ferrochelatase three-dimensional structure, and is critical for enzyme activity. (unl.pt)
Approach1
The screening approach not only identified the previously reported hBChE G117H variant, but also identified a series of additional hBChE variants, including hBChE G117N, G117R, E197C, and L125V, that exhibited OP-resistant functional activities not reported previously. (aspetjournals.org)
Hydrolytic1
Previously, rationally designed hBChE mutants (G117H and E197Q) were reported in the literature and showed the feasibility of engineering OP hydrolytic functional activity into hBChE. (aspetjournals.org)
Electrochemical2
Scanning electrochemical microscopy (SECM) has been used to image and study the catalytic activity of horseradish peroxidase (HRP) immobilised in a patterned fashion onto glass slides. (cranfield.ac.uk)
6] M. Březina, Estimation of electrochemical activity of carbon using a paste electrode. (edu.pl)
Vivo2
An AA5 enzyme from Arabidopsis thaliana has been demonstrated in vivo to have galactose 6-oxidase activity and promote cell-to-cell adhesion in the seed coat epidermis [ 15 ] (see also [ 16 ]. (cazypedia.org)
The geometry of the active site (three classes have been described), the possible electron donors in vivo and the current hypothesis for the catalytic mechanism will be discussed. (unl.pt)
Ascenzi P, Tundo GR, Coletta M. (2018) The nitrite reductase activity of ferrous human hemoglobin:haptoglobin 1-1 and 2-2 complexes . (uniroma2.it)
Human butyrylcholinesterase (hBChE) is currently being developed as a detoxication enzyme for the catalytic hydrolysis or stoichiometric binding of organophosphates (OPs). (aspetjournals.org)
Carbon1
In this review (with 500 refs), both electrochemistry and electroanalysis with carbon paste-based electrodes, sensors, and detectors are of interest, when attention is focused on the research activities in the years of new millennium. (edu.pl)
Mechanism1
Taken together, these findings suggest that the structural strain imposed by ferrochelatase on the porphyrin substrate as a critical step in the enzyme catalytic mechanism is also accomplished by the E287A and E287Q variants, but without the release of the product. (unl.pt)
Domain1
GCN2 contains an RWD domain (aa 21-134), a pseudo-kinase domain (aa 296-539), a catalytic domain (aa 590-1001), and a histidyl tRNA synthase-like domain (aa 1022-1493) followed by a C-terminal ribosome interacting region. (rndsystems.com)
Variants3
As described herein, a directed molecular evolution process was used to identify amino acid residues that contribute to OP-resistant functional activity of hBChE variants. (aspetjournals.org)
Immediate kinetic analysis making use of floor plasmon resonance (SPR) as well as glycoproteins holding high-mannose carb organizations (RNase N, horseradish peroxidase and also mannan-BSA) revealed the existence of high- as well as low-adhesive allelic variants, not only in Azines. (phosphorylasesignals.com)
Substitution of E287 with either glutamine (Q) or alanine (A) yielded variants with lower enzymic activity than that of the wild-type ferrochelatase and with different absorption spectra from the wild-type enzyme. (unl.pt)
Nature1
Co-doping of nitrogen and boron into graphene, which has negligible peroxidase-like activity, selectively increased the peroxidase-like activity without oxidase-like activity to accurately mimic the nature peroxidase and has become a powerful candidate to replace the peroxidase. (k-erc.eu)
Increases1
Combining in vitro studies with studies in cell models, we found that this mutation reduces aSyn aggregation and increases proteasome activity, altering normal proteostasis. (biomedcentral.com)
Cell1
Recently, we reported that the ubiquitin E3 ligase ITCH negatively regulates LATS1, thereby increasing YAP activity, which leads to increased cell proliferation and decreased apoptosis. (oncotarget.com)
Center1
Its catalytic phosphatase active center is structurally similar to phosphatase, so it is named PTEN . (aging-us.com)
Specific1
HLE-B3 cells, maintained in a continuous hypoxic environment (1% oxygen), were treated with SB216763, a specific inhibitor of glycogen synthase kinase-3β (GSK-3β) catalytic activity. (molvis.org)
Cells1
Stimulation of PF cells with ↑[Ca 2+ ] e increased the activity of immunoprecipitated PKCζ (but not PKCι/λ), and the activity of this PKCζ was inhibited by pseudoZ. (jneurosci.org)
Research1
A KAIST research team doped nitrogen and boron into graphene to selectively increase peroxidase-like activity and succeeded in synthesizing a peroxidase-mimicking nanozyme with a low cost and superior catalytic activity. (k-erc.eu)