• With these precedents, you might think that designing catalytic proteins should be straightforward, but success has been limited. (nature.com)
  • Catalytically inactive proteins have been converted into modestly catalytic ones for two different reactions, but the observed enhancements of rate 6 , 7 were only about a millionth of those produced by naturally occurring enzymes. (nature.com)
  • The main limiting factor is the efficiency, speed and quantity of the enzymes that build psilocybin and its genetics that codes for the proteins that build the enzymes that builds the magic. (shroomery.org)
  • Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified. (bvsalud.org)
  • Transcriptomic analysis of the two recombinant strains in comparison with the control strain showed that the correct folding of proteins assisted by molecular chaperones played a key role in the improvement of FumDM expression and its enzyme activity. (inra.fr)
  • Although enzymes are superb catalysts, their range of reactions is limited to those that support life. (nature.com)
  • Enzymes are astoundingly good catalysts: they allow reactions to occur billions of times faster than would be possible without them, at temperatures much lower than those required by typical synthetic catalysts. (nature.com)
  • We modelled how open-circuit voltage optimizes platinum-loading in catalysts to improve the electrochemical activity (ECA) possible from bioelectrogenesis through these systems and address the high costs of nano-drug delivery systems. (scielo.org.za)
  • The effects of the catalysts' convective flux and proton concentration were modelled for an enzyme (glucose oxidase) biofuel cell that was fed glucose substrate at a current rate under isothermal physiological conditions. (scielo.org.za)
  • Here we aimed to investigate a hybrid catalytic model (biological and metal catalysts) where the biological catalyst can enhance the anode's open circuit voltage (OCV) by supplying free (solvated) electrons and protons 1 to optimize the metal's OCV for a fuel cell, which is also a nano-drug delivery system (NDDS). (scielo.org.za)
  • Replacing enzymes with more stable and cheaper oxidation catalysts will reduce the cost of glucometers and extend their shelf life. (eurekalert.org)
  • To date, only one method has been developed for direct blood analysis using enzyme-free catalysts based on palladium nanoparticles. (eurekalert.org)
  • Thus, scientists are faced with the task of synthesizing enzyme-free catalysts, which will make it possible to more accurately and with lower financial costs determine the concentration of glucose in the blood. (eurekalert.org)
  • Because the synthetic method requires no organic solvent and because of the distinct hierarchical nanostructure, protein-inorganic nanoflowers display enhanced catalytic activity and stability and would be a promising tool in biocatalytical processes and biological and biomedical fields. (cdc.gov)
  • What also complicates matters in this area is the fact that data describing enzymatic experiments is particularly complex, because an enzymatic reaction depends on many factors, such as the protein sequence of the enzyme, the recombinant host organism, the reaction conditions, and non-enzymatic secondary reactions. (uni-stuttgart.de)
  • An essential step in demonstrating the success of a designer enzyme, therefore, is the determination of a high-resolution crystal structure for the protein, to verify that the designed catalytic features are present. (nature.com)
  • In parallel, we used hydrogen-deuterium exchange (HDX) coupled with mass spectrometry (MS) approach to gain molecular insights into ancHLD-RLuc protein dynamics, and identification of conformationally rich regions that could be important for the catalytic reaction. (europa.eu)
  • Since enzymes are of a protein nature, they denaturate under heat or light exposure, and their shelf life is limited. (eurekalert.org)
  • The physical chemistry of enzyme confinement, methods of immobilization, catalytic activity and advantages of protein confinements are discussed. (tmu.edu.tw)
  • The recombinant protein, overexpressed in Escherichia coli, exhibits a low catalytic activity and an unexpectedly high thermal stability (Tm = 64.8 degrees C). Based on various spectroscopic data, on the known three-dimensional structure of the AK from E. coli and on secondary structure predictions for various sequenced AKs, we propose a structural model for AK from M. tuberculosis (AKmt). (rhea-db.org)
  • Two SNVs affect the protein structure in several ways, including reduction of catalytic activity and reduction in stability and expression of the full-length transcript. (ectrx.org)
  • CK, formerly known as creatinine phosphokinase, is an important protein enzyme catalyzing the reversible phosphorylation reaction. (medscape.com)
  • Allergen avoidance strategies including personal protective equipment, engineering controls, protein encapsulation, and reduction of airborne enzyme concentrations are required to mitigate occupational exposure to fungal enzymes. (cdc.gov)
  • Enzyme immobilisation technology is an effective means to improve sugar ester production through the employment of biocatalysts. (researchgate.net)
  • 3 Biocatalysts (GOx enzymes) are employed in enzyme fuel cells containing anodic (oxidation) and cathodic (reduction) chambers. (scielo.org.za)
  • In the end, we show that immobilized enzymes in the nanospaces of MPS can be applied as viable biocatalysts for chemical and pharmaceutical industries. (tmu.edu.tw)
  • Enzymes are versatile biocatalysts that fulfill essential functions in all forms of life and, therefore, play an important role in health and disease. (kth.se)
  • But enzymes have evolved to accelerate only biological reactions, under the narrow set of conditions that are compatible with life. (nature.com)
  • They describe a method for designing enzymes that catalyse unnatural reactions, and demonstrate its use for two different chemical transformations. (nature.com)
  • Enzymes work by lowering the activation energy of reactions, specifically by confining substrates in binding sites that stabilize the highest-energy arrangement of atoms in the reaction pathway (known as the transition state). (nature.com)
  • Furthermore, transition states are modelled using quantum-mechanical calculations, but currently available methods can handle only a limited number of atoms, and are often inadequate for modelling enzyme reactions. (nature.com)
  • GOx (enzyme) is a natural catalyst that helps in speeding up chemical reactions without being used or depleted during the reactions. (scielo.org.za)
  • For example, Degussa AG (now Evonik AG) reported a LeuDH and FDH coupling system to perform repeated batch reactions with an enzyme membrane reactor on a ton scale and the space-time yield of l -tle achieved 638 g/L/day [ 14 ]. (biomedcentral.com)
  • Based on the acetylcholinesterase/choline oxidase dual enzyme-mediated cascade catalytic reactions of Fe(2+)/Fe(3+) transformation, a ratiometric fluorescence sensing scheme is developed. (inra.fr)
  • 2) the nanoflower has enhanced enzymatic activity and stability to produce an amplified signal. (cdc.gov)
  • Third, it has enhanced enzymatic stability compared to the free form of enzyme due to the unique hierarchical nanostructure. (cdc.gov)
  • This also applies to studies on the catalytic activity, selectivity and stability of enzymes and enzymatic networks, a field of research that is equally important for industrial biotechnology and biomedicine. (uni-stuttgart.de)
  • The format thus provides a seamless communication channel between experimental platforms, electronic lab notebooks, enzyme kinetics modeling tools, publication platforms, and enzymatic reaction databases. (uni-stuttgart.de)
  • These cells also develop specific strategies to increase ROS resistance and to express the enzymatic activities necessary for ROS detoxification. (hindawi.com)
  • The overall enzymatic biological fuel cell's electrochemical activity (BECA) depends on efficient OCV generation and optimized kinetics. (scielo.org.za)
  • Moreover, the enzymes created by ASR often exhibit enhanced thermal stability and promiscuous enzymatic properties, which can be useful in various industrial settings. (europa.eu)
  • The prepared antibody-enzyme-inorganic nanoflower was first applied in an enzyme-linked immunosorbent assay to serve as a novel enzyme-labeled antibody for Escherichia coli O157:H7 (E. coli O157:H7) determination. (cdc.gov)
  • Cel5E mutations were inserted into celH by overlapping polymerase chain reaction, followed by expression of wild-type and mutant enzymes in Escherichia coli BL21 (DE3) and purification by affinity chromatography. (irost.org)
  • The enzyme confinements in the nanochannels of MPS materials generate synergistic effects that enhance enzyme stability, improve product selectivity, and facilitate separation and reuse of enzymes. (tmu.edu.tw)
  • The enzyme activities of recombinant strains over-expressing PDI and CPR5 reached the highest levels of 259.47 U/mL and 161.34 U/mL, 635% and 357% higher than the original enzyme activity, respectively. (inra.fr)
  • abstract = "The unique properties of mesoporous silica (MPS) materials were utilized to immobilze enzymes: huge surface area, modifiable surface, and restricted pore nanospaces. (tmu.edu.tw)
  • 1 have computationally designed and prepared the first enzyme capable of catalysing a non-biological reaction. (nature.com)
  • Rational bioengineering attempts to create new RLuc variants with fine-tuned bioluminescent properties are however hampered by the fact that its catalytic reaction mechanism remains poorly understood. (europa.eu)
  • Poor crystallibility and long-term (>3 months) crystallization process of RLuc appeared to be major drawbacks in the acquisition of structural data that should provide the molecular dissection of RLuc catalytic reaction mechanism. (europa.eu)
  • We have immobilized Candida antarctica lipase B (CALB) on different resins in order to obtain a more cost-effective biocatalyst, i.e. to find the cheapest preparation per catalytic activity, for one esterification and one amidation reaction. (lu.se)
  • One of the major intentions of this project was to determine atomic-level structures of the dual-function ancestral enzyme ancHLD-RLuc complexed with bound substrate molecules, with emphasis on coelenterazine-powered monooxygenation (luciferase) catalysis. (europa.eu)
  • We succeeded to reconstitute stable complexes of recombinantly-produced and affinity-purified ancHLD-RLuc enzyme complexed with substrate-like and product molecules. (europa.eu)
  • Unlike the native coelenterazine, the derivative cannot be oxidized, and therefore it represents a perfect chemical tool to study enzyme-substrate complexes. (europa.eu)
  • These studies allowed the tracing of the previously disordered region of the enzyme (Glu196-Arg202) and the identification of some of the functional groups of the enzyme that are involved in enzyme-substrate interactions (Asp160, Met161, Gly201, Arg202 and Phe219). (huji.ac.il)
  • PON1 can be evaluated by its different activities , for example its paraxonase activity (when paraoxon is used as substrate), arylesterase activity (when a non phosphorous arylester such as phenyl acetate or 4 (p)-nitrophenyl acetate is used as substrate) or by its lactonase activity (when 5-thiobutil butyrolactone -TBBL- or other lactones such as dihydrocoumarin are used as substrate). (biomedcentral.com)
  • In Paper I , we established the utility of this method in a model system and obtained ancestral terpene cyclases with increased activity, stability and substrate scope. (kth.se)
  • The endogenous substrate and the mechanisms of the antiatherogenic activity of this enzyme are still largely unknown. (biochemia-medica.com)
  • So it is assumed that the native activity of PON1 may be lactonase activity and that physiological substrate may be some lactones which are consumed as food ingredients, drug metabolites (statins, spironolactone and glucocorticoid γ-lactones) and derivatives of fatty acid oxidation process such as 5-hydroxy6E,8Z,11Z,14Z-eicosatetraenoic acid (5-HETE) lactone that resides in HDL (13). (biochemia-medica.com)
  • As the thiolate anion is not energetically favoured in the hydrophobic interior of the enzyme, it swings into the active site where it can be stabilized by the His202 imidazolium and the dipole of helix C. The Cys123 thiolate which now lies adjacent to the acyl carbon of the substrate, is thus generated only in the presence of substrate. (inrae.fr)
  • We targeted a semi-hydrophobic pocket near the Cel5E active site and theoretically screened mutated variants for enhanced levels of thermal stability. (irost.org)
  • Compared with the free enzymes, both the environmental tolerance and thermal stability of GDH-R3-LeuDH had a great improved since the fusion structure. (biomedcentral.com)
  • Specifically, our structures highlight: (i) amino acid residues involved in chemical steps, (ii) residues that deprotonate/reprotonate the coelenteramide to yield blue light (480 nm), and (iii) coordinated motions of enzyme loops carrying aromatic residues that are important for the bulky ligand binding-unbinding turnover. (europa.eu)
  • It is a double-zinc enzyme with a strong preference for large hydrophobic amino-terminus residues. (huji.ac.il)
  • The root-mean-square deviation from ideal bond distances for the model is 0.014 A and the error in atomic co-ordinates is estimated to be 0.15 A. DLH is a monomeric enzyme containing 236 amino acid residues and is a member of the beta-ketoadipate pathway found in bacteria and fungi. (inrae.fr)
  • However, the poor structural stability and the high cost of purification and immobilization of free enzyme system limit the industrial application. (biomedcentral.com)
  • The resulting new material has significant catalytic activity in the electrochemical oxidation of glucose. (eurekalert.org)
  • Therefore, MOFs can be used as effective templates for the preparation of hollow and porous TMOs with high ion transport efficiency, excellent specific capacitance, and electrochemical activities. (frogheart.ca)
  • Biological applications with a focus on permeability enhancing, antimicrobial activity, and antibiofilm properties of 6′-lactose-based esters are also reported. (researchgate.net)
  • For this bioluminescent effect, the RLuc luciferase is commonly used as a reporter enzyme in cell biological research and bioimaging technologies. (europa.eu)
  • Biological molecules that possess catalytic activity. (bvsalud.org)
  • It was found that lipase immobilized on macroporous polypropylene (Accurel MP1000) and polymethylmethacrylate (Lewatit) provided biocatalyst preparations where the cost of enzyme and carrier was significantly less than the cost of commercially available Novozym (R) 435, indicating a potential for decreased cost. (lu.se)
  • Finally the in-house biocatalyst was used in a packed-bed set-up, showing an excellent stability in repeated batches at 70 degrees C. 2010 Elsevier B.V. All rights reserved. (lu.se)
  • Free and immobilised lipases were tested for their esterification activities. (researchgate.net)
  • Other enzymes and lipases from other microorganisms were also tested in the biocatalytic esterification of mono-and disaccharides. (researchgate.net)
  • In oxidative stress conditions, the catalytic cysteine site is transformed to a sulfenate which inhibits the MPST enzyme activity. (prospecbio.com)
  • A deficiency in MPST activity is implicated in a rare inheritable condition known as MCDU (mercaptolactate-cysteine disulfiduria). (prospecbio.com)
  • Like the cysteine/serine proteases, DLH has a catalytic triad (Cys-123, His-202, Asp-171) and its mechanism probably involves the formation of covalently bound acyl intermediate via a tetrahedral intermediate. (inrae.fr)
  • It is a monomeric (30 kDa) heat-stable enzyme, with a high and efficient catalytic activity modulated by calcium ions. (huji.ac.il)
  • These are enzymes glucose oxidase or glucose dehydrogenase, which are produced by microorganisms. (eurekalert.org)
  • It has not been observed in other hydrolytic enzymes and it is virtually inactive when it is produced by site-directed mutagenesis in the proteases. (inrae.fr)
  • The thermodynamic stability of every sequence and conformation must therefore be calculated to find the lowest-energy structure (that is, the one most likely to form). (nature.com)
  • Interestingly, HLD enzymes display remarkable sequence and structural similarity with luciferase from the marine invertebrate Renilla reniformis (RLuc), which reflects their common evolutionary history. (europa.eu)
  • To overcome these limitations, ancestral sequence reconstruction (ASR) represents a powerful approach, in which a hypothetical ancestral sequence of a given present-day enzymes is predicted and reconstructed in a laboratory. (europa.eu)
  • AAP) has been structurally analyzed to date and its structure was shown to be considerably similar to SGAP, despite the low sequence homology between the two enzymes. (huji.ac.il)
  • The aim of the work presented in this thesis is to enhance the therapeutic potential of enzymes by means of enzyme engineering, in particular using ancestral sequence reconstruction. (kth.se)
  • In summary, the results presented herein highlight the potential of ancestral sequence reconstruction as a method to obtain stable enzyme scaffolds for further engineering and to enhance therapeutic properties of enzymes. (kth.se)
  • A sequence comparison with 52 different forms of adenylate kinases (AK) suggests that the enzyme from M. tuberculosis belongs to a new subfamily of 'short' bacterial AKs. (rhea-db.org)
  • A sequence variant had no observable enzyme activity. (ectrx.org)
  • Interestingly, the optimal temperature for CMCase activity by the N94W variant was shifted 2 °C higher than that for the wild-type enzyme. (irost.org)
  • The small size, high activity and heat stability make SGAP a very attractive enzyme for various biotechnological applications. (huji.ac.il)
  • Each subunit contains two molecules of zinc, necessary for stability and catalytic activity. (scrippslabs.com)
  • However PON1 is considered as a promiscuous enzyme having also the ability to hydrolyze many other substrates such as other organophosphorous compounds, non phosphorous arylesters and also lactones, which have been considered as its primary substrates [ 19 ]. (biomedcentral.com)
  • Maybe it would be more appropriate to use the general term "PON1 activity" that can be measured by different substrates such as paroxon, phenyl acetate, 4-nitrophenyl acetate, TBBL or dihydrocoumarin. (biomedcentral.com)
  • PON1 possess organophosphatase, arylesterase and lactonase activity and it hydrolyzes many different substrates. (biochemia-medica.com)
  • PON1 possess organophosphatase, arylesterase and lactonase activities and hydrolyzes different kinds of substrates. (biochemia-medica.com)
  • These studies also suggest that Glu131 is directly involved in the catalytic mechanism of SGAP, probably as the hydrolytic nucleophile. (huji.ac.il)
  • The motivation for the detailed structural analysis of SGAP originated from a strong mechanistic interest in the family of double-zinc aminopeptidases, combined with the high potential applicability of these enzymes. (huji.ac.il)
  • Furthermore, other effects such as inactivation or inhibition of the enzyme, or evaporation of the medium affect the results. (uni-stuttgart.de)
  • 1984. Theoretical aspects of enzyme induction and inhibition leading to the reversal of resistance to biocides. (cdc.gov)
  • Trimethoxysilyl and acrylamide-bisacrylamide polymers have been used for synthesis of enzyme nanoparticles. (mdpi.com)
  • Preserving Catalytic Activity and Enhancing Biochemical Stability of the Therapeutic Enzyme Asparaginase by Biocompatible Multi layered Polyelectrolyte Microcapsules. (mpg.de)
  • It is also sometimes difficult to prove that designer enzymes are truly catalytic on the basis of biochemical observations, and some exciting claims have been found to be flawed. (nature.com)
  • Paraoxonases are a family of three enzymes called PON1, PON2 and PON3. (biomedcentral.com)
  • The products of those three genes are the following enzymes: paraoxonase 1 (PON1), paraoxonase 2 (PON2) and paraoxonase 3 (PON3). (biochemia-medica.com)
  • In our scientific activity at the Division of Physical Chemistry 1 we find it essential to establish long-term goals and policies that are based on an unbiased analysis of the specific situation, rather than an uncritical following of the current trends. (lu.se)
  • The MPST enzyme is regulated by oxidative stress and thioredoxin. (prospecbio.com)
  • Paraoxonase 1 (PON1) is a widely studied enzyme based on its protective role against poisoning by organophosphate (OP) metabolites of specific OP insecticides and in vascular disease, as well as its use as biomarker of diseases involving oxidative stress, inflammation and liver diseases. (biomedcentral.com)
  • We showed that a non-oxidizable coelenterazine analogue binds to the ancHLD-RLuc enzyme with low nanomolar affinity. (europa.eu)
  • Psilocin is a bit different as it it's not in the synthesis but from exposure of psilocybin to PsiK a dephosphorelating enzyme. (shroomery.org)
  • The cofactor regeneration system of leucine dehydrogenase (LeuDH) and glucose dehydrogenase (GDH) has showed great coupling catalytic efficiency in the synthesis of l -tle, however the multi-enzyme complex of GDH and LeuDH has never been constructed successfully. (biomedcentral.com)
  • Specific activities for Amino-CRL and NER-CRL increased by 2.4 and 2.6-fold, respectively, upon immobilisation. (researchgate.net)
  • The optimised conditions for sugar fatty acid ester syntheses are 48 h at 2:1 of molar ratio of lactose sugar to capric acid at 55 °C. Furthermore, a high operational stability with half-lives of over 13 and 10 runs was achieved for NER-CRL and Amino-CRL, respectively, indicating the efficiency of the immobilisation process. (researchgate.net)
  • The adk gene from Mycobacterium tuberculosis codes for an enzyme of 181 amino acids. (rhea-db.org)
  • Second, it has fairly high capture capability and catalytic activity because it is presented as aggregates of abundant antibodies and enzymes. (cdc.gov)
  • This enzyme is useful as an indicator enzyme when coupled to antibodies used in enzyme-based immunoassay systems. (scrippslabs.com)
  • PON1 can be measured based on its activity by spectrophotometric assays and also can be directly quantified by immunological methods using specific antibodies [ 17 ]. (biomedcentral.com)
  • Immunologically mediated OA accounts for approximately weight allergens in industrial settings and describe the 90% of cases [9], but the severity of disease is dependent main enzymes that have been associated with occupational on the concentration, route, agent of exposure, and the sensitization and asthma. (cdc.gov)
  • Finally, we applied the method to two therapeutically relevant enzyme families associated with rare metabolic disorders. (kth.se)
  • Using the free (solvation) electron model, bioelectrochemical activity (BECA) and a high open circuit voltage were generated by 5.5,10 and 20 mM glucose with 20 kU L -1 glucose oxidase at 37 °C. BECA (glucose oxidase), on its own, produced pulses of various intensities for nano-microsecond durations whereas the hybrid BECA-ECA (glucose oxidase and platinum) anode catalyst provided sustainable pulses of microseconds-minute durations. (scielo.org.za)
  • In the process of LeuDH production of l -tle, NADH as a cofactor is indispensable and expensive, so an efficient cofactor regeneration system was considered as an ideal solution with high catalytic and economic benefits [ 11 ]. (biomedcentral.com)
  • One specific application of enzymes in life science is their use as biopharmaceuticals, which typically benefits from high catalytic activity and stability. (kth.se)
  • More importantly, this work provides a new and feasible strategy for the efficient preparation of ECMs with fast response speed and high cyclic stability. (frogheart.ca)
  • 6]. Some of the best examples of high-molecular-weight sectors, workers are at increased risk of becoming sensitized occupational allergens are the fungal enzymes. (cdc.gov)
  • A small amount of this enzyme is also bound to very low-density lipoprotein (VLDL) and postprandial chylomicrons. (biochemia-medica.com)
  • Also the stability of Accurel MP1000 bound enzyme during repeated use matched that of Novozym (R) 435. (lu.se)
  • able to live on plants, it must have possessed a cyanide-detoxifying enzyme. (researchwap.net)
  • Biocatalytic kinetic effect of α -chymotrypsin enzyme has been investigated in its free and pretreated forms (it was covered by a very thin, porous polymer layer, called enzyme nanoparticle) as well as its immobilized form into pores of polysulfone/polyamide asymmetric, hydrophilic membrane. (mdpi.com)
  • The reconstituted ancestral enzymes have been shown to be valuable tools in our understanding of the evolution of biocatalytic mechanisms. (europa.eu)
  • Based on our unique structural data we were able to propose the catalytic mechanism of conversion of coelenterazine into coelenteramide, accompanied with emission of blue light, at alpha/beta-hydrolase fold. (europa.eu)
  • We investigated two parameters as power sources for a nano-drug delivery system to simultaneously enhance drug-membrane permeation: the first one was the glucose oxidase (GOx) bioelectrochemical activity (BECA) at low glucose concentrations, and the second was a combined system, BECA electro-chemical activity (ECA) model (BECA-ECA), with platinum-nanoparticle (Pt-NP) anti-microbial and anti-cancer action. (scielo.org.za)
  • The three-in-one antibody (anti-E. coli O157:H7 antibody)-enzyme (horseradish peroxidase)-inorganic (Cu3(PO4)2) nanoflower has some advantages over commercial enzyme-antibody conjugates. (cdc.gov)
  • designed, synthesized, and investigated the effects of a cationic comb polymer on the stability of aqueous silica suspensions of varying ionic strength and pH. (rpi.edu)
  • Enzymes induced in 4-fluorobenzoate-utilizing bacteria have been classified into three groups on the basis of their specificity towards cis- and trans-dienelactone. (inrae.fr)
  • We hypothesize that, in the future, the hybrid-catalyst assembly will be made using the membrane electrode assembly (MEA) with layered nano-particles and an enzyme hybrid assembly. (scielo.org.za)
  • The catalytic activity of palladium is enhanced in combination with silver: two elements, co-applied to the electrode portion of the test strip, improve each other's catalytic properties. (eurekalert.org)
  • The new system, the developers say, can be used to create an enzyme-free electrocatalytic sensor for determining glucose in whole blood. (eurekalert.org)
  • Possible substitution options include palladium (it has stability and increased sensitivity to glucose). (eurekalert.org)
  • In some occupational environments, workers are at an increased risk of exposure to fungal enzymes used in industrial production. (cdc.gov)
  • Exposure-response relationships have been demonstrated, and atopic workers directly handling fungal enzymes are at an increased risk for IgE-mediated disease and occupational asthma. (cdc.gov)
  • Their repertoire could be expanded by a method that allows artificial enzymes to be made from scratch. (nature.com)
  • As the large specific surface areas and hollow porous nanostructures were conducive to ionic transport, PYS-NiO NSs exhibited a fast coloring/bleaching speed (3.6/3.9 s per one coloring/bleaching cycle) and excellent cycling stability (82% of capacity retention after 3000 cycles). (frogheart.ca)
  • Besides, PYS-NiO NSs also exhibited excellent cycling stability (82% of capacity retention after 3000 cycles). (frogheart.ca)
  • 2 are remarkable in the spectacular agreement between their computationally predicted enzyme models and the experimentally determined structures ( Fig. 1 ). (nature.com)
  • So the authors proposed a new strategy to design a kind of NiO with hollow and porous structure to obtain excellent EC performance and cyclic stability. (frogheart.ca)
  • We evaluated a hybrid catalytic power source for less invasive internal electroporation with better tissue reach than the widely used and more invasive external electroporation. (scielo.org.za)
  • Furthermore, it is well known that PON1 possess antiatherogenic activity, it protects HDL and low-density lipoprotein (LDL) from oxidation and destroys biologically active oxidized lipids on lipoproteins and in arterial cells (2,12,13). (biochemia-medica.com)
  • Initially the interest on this enzyme arose from the toxicological point of view, by its protective role from poisoning by organophosphate derivates. (biomedcentral.com)
  • 6,7 Inosine triphosphate pyrophosphates (ITPases) represent a ubiquitous group of protective enzymes encoded by the ITPA gene and function to prevent the incorporation of noncanonical (so-called rogue) purine nucleotides into DNA and RNA. (ectrx.org)
  • LeuDH and FDH with PDZ (PSD95/Dlg1/Zo-1) domain and the corresponding ligand of the metazoan (PDZlig) were fused and assembled as multiple supramolecules to improve the NAD(H) recycling efficiency and structural stability [ 19 ]. (biomedcentral.com)
  • PON1 is the most studied enzyme of the family. (biomedcentral.com)
  • In addition, it has been reported that PON1 has low levels of peroxidase and phospholipase A2-like activities (2,3,7-11). (biochemia-medica.com)
  • A new subfamily of short bacterial adenylate kinases with the Mycobacterium tuberculosis enzyme as a model: a predictive and experimental study. (rhea-db.org)
  • Loading capacity versus enzyme activity in anisotropic and spherical calcium carbonate microparticles. (mpg.de)