Glutathione ReductaseEnzymes1.11.1.21H2O2AssayMetabolismActivitiesSignificantlyOxygenActivityLiverDismutaseLevelsResultMycobacteriumStimulated catalase activityGlutathione reductaseKatGAscorbate peroxidaseEnzymeHemeDismutasesIntracellularCytochromeProteinEscherichiaHydrogenSODsMitochondrialOxidative stressAntioxidantsNanoparticlesGeneTemperatureReactionFoundStudyPresent
Glutathione Reductase2
- Changes in the activities of glutathione peroxidase (GSH-Px): glutathione reductase (GSH-R), and catalase, and in the content of reduced glutathione (GSH) in blood samples were determined by means of biochemical methods. (nih.gov)
- To this end, many assay methods have been developed for the detection of ROSrelated or ROS-derived substances such as superoxide anion, superoxide dismutase, glutathione, glutathione reductase, glutathione peroxidase, DNA lesions, 8-oxoguanine, 8-nitroguanosine, and protein carbonyl. (dojindo.com)
Enzymes8
- This study was conducted to evaluate the effect of radiofrequency wave (RFW)-induced oxidative stress in the eye and the prophylactic effect of vitamin C on this organ by measuring the antioxidant enzymes activity including: glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), and malondialdehyde (MDA). (nih.gov)
- Epididymal (visceral) and subcutaneous adipose tissue, gastrocnemius muscle, and plasma samples were collected to measure oxidative stress markers (advanced oxidation protein products (AOPP), oxidized low-density lipoprotein (oxLDL)), antioxidant system markers (ferric-reducing ability of plasma (FRAP), superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx) activities), and prooxidant enzymes (NADPH oxidase and xanthine oxidase (XO) activities, myeloperoxidase content). (hindawi.com)
- The aim of this study was to determine the effect of acute intoxication with chlorfenvinphos on the liver reduced glutathione (GSH) level and the activities of two enzymes involved in glutathione metabolism, as well as the activity of catalase (CAT), glucose-6-phosphate dehydrogenase (G6PDH) and the level of liver hydrogen peroxide. (pjoes.com)
- In order to understand the role of various enzymes in the protection of red cells against oxidative damage, SOD, GSH-Px, GSH reductase, and both the catalatic and peroxidatic activities of catalase were determined in acatalasemic, GSH-Px-deficient mice. (utmb.edu)
- Activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) were measured in ellagic acid-treated V79-4 cells. (nutrimedical.com)
- Among antioxidant enzymes, catalase, superoxide dismutase, and glutathione peroxidase were decreased and lipid peroxidation was increased with regional alterations. (fluoridealert.org)
- There were no changes in protein content for GPx-1 and catalase or messenger RNA for any of the enzymes studied. (cdc.gov)
- In order to prevent oxidative damage, the antioxidant system presents a group of cellular enzymes (SOD, catalase and glutathione system) responsible for the control of free radicals. (bvsalud.org)
1.11.1.211
- Catalase-peroxidase (EC 1.11.1.21, katG (gene)) is an enzyme with systematic name donor:hydrogen-peroxide oxidoreductase. (wikipedia.org)
H2O24
- This enzyme catalyses the following chemical reaction donor + H2O2 ⇌ oxidized donor + 2 H2O 2 H2O2 ⇌ O2 + 2 H2O This enzyme is a strong catalase with H2O2 as donor which releases O2. (wikipedia.org)
- The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. (nih.gov)
- therefore, by limiting H2O2 accumulation, glutathione peroxidases are also involved in modulating these processes. (nih.gov)
- While SOD catalyzes the dismutation of superoxide anion (O2') to H2O2, catalase and glutathione (peroxidase, reductase) system reduce cellular toxicity degrading peroxides into oxygen and water 9 . (bvsalud.org)
Assay3
- The liver glutathione peroxidase (GPx) and reductase (GR) activities as well as GSH and hydrogen peroxide levels were determined using Bioxytech Assay kits. (pjoes.com)
- Chance B, Maehly A (1995) Assay of catalase and peroxidase. (springer.com)
- Assay of glutathione peroxidase in the heart after 6 wk of selenium depletion with both hydrogen peroxide and cumene hydroperoxide as substrates revealed a >80% drop in enzyme activity and gave no indication that murine cardiac tissue contains nonselenium-dependent glutathione peroxidase. (jci.org)
Metabolism1
- Metabolism of glutathione peroxidase, can further inflammation, and - along with oth- many exogenous agents through cy- convert these reactive species to er radical species - play key roles tochrome P450-mediated reactions wate r (Benzie, 2000) . (who.int)
Activities12
- Chronic ethanol ingestion elicited a significant increase in GSH-Px activity (by a maximum of approximately 32% on day 14), whereas opposite alterations in GSH-R and catalase activities were recorded (49% of the control value on day 4 and 17% on day 21, respectively). (nih.gov)
- Compared with the control, MICT increased GPx and catalase activities and the FRAP level in epididymal adipose tissue. (hindawi.com)
- Moreover, the application of the investigated 75 μM MJ significantly improved enzyme activities in terms of catalase, peroxidase and ascorbate peroxidase activities. (springer.com)
- Lactate dehydrogenase (LDH) and creatine kinase‑MB (CK‑MB) release, infarct size, cardiac function, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities, malondialdehyde (MDA), intracellular reactive oxygen species (ROS), and DJ‑1 protein expression levels were assessed. (spandidos-publications.com)
- Characterization of Catalase Activities in a Root-Colonizing Isolate of 'Pseudomonas putida' (Revised). (epa.gov)
- Glutathione peroxidase activities were, however, similar to the two tissues. (jci.org)
- The higher protective capacity of BEH on SOD, catalase and peroxidase activities was comparable to that of the vitamin C used as standard. (degruyter.com)
- With increasing salinity levels, leaf superoxide dismutase (SOD) activity and lipid peroxidation generally increased, but catalase (CAT) and peroxidase (POD) activities showed non-linear patterns. (researchsquare.com)
- Malondialdehyde (MDA) level, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were measured. (medscimonit.com)
- 0.0001) glutathione-(GSH), GSH-peroxidase/S-transferase, and catalase erythrocyte activities. (avaate.org)
- However, no changed were observed in in blood carotenoid and tocopherol levels, nor in the activities of glutathione peroxidase and catalase. (nutraingredients-usa.com)
- Malondialdehyde and nitric oxide levels were significantly higher in the lungs and liver, while the activities of superoxide dismutase, glutathione peroxidase-1, and catalase were significantly lower in the waterpipe smoke group when compared to control mice. (who.int)
Significantly1
- The selenium-deficient state, which was characterized by markedly decreased cardiac glutathione peroxidase levels, led to significantly enhanced doxorubicin toxicity at a dose of 15 mg/kg i.p. (jci.org)
Oxygen3
- The whole medium and posterior intestine was collected after 30 or 60 experimental days and the activity of catalase, superoxide dismutase and glutathione peroxidase was determined as one international unit per milligram of protein, and the oxygen radical absorbance capacity as µM of equivalent Trolox per milligram of protein. (lajar.cl)
- Moretti D, Nordi W, Cruz T, Machado-Neto R. Catalase, superoxide dismutase, glutathione peroxidase and oxygen radical absorbance capacity in the gut of juvenile pacu Piaractus mesopotamicus and dourado Salminus brasiliensis fed bovine first milk secretion. (lajar.cl)
- to superoxide or that "autoxidize", and when coupled with catalase Oxygen radicals clearly contribute to probably through reactions catalysed within peroxisomes or with cytosolic genomic instability, are produced by by transition metals. (who.int)
Activity6
- A deficiency in the catalatic activity of catalase, GSH-Px, or both did not affect the red cell survival. (utmb.edu)
- Although the catalatic activity of catalase was deficient in the red cells of the acatalasemic mice (C3H-C Sb -ANL), the peroxidatic activity of catalase was comparable to that of the normals. (utmb.edu)
- It is possible that the catalatic activity of catalase does not play a significant role in the protection of the red cell against oxidative damage. (utmb.edu)
- However, GSH-Px and possibly the peroxidatic activity of catalase are necessary to maintain a reduced atmosphere in the red cell under oxidative stress. (utmb.edu)
- while decline in catalase (CAT) activity was recorded when compared with the untreated control. (scirp.org)
- Glutathione peroxidase (GPx) activity decreased whereas catalase activity increased with RL in muscles from young and aged rats. (cdc.gov)
Liver1
- cardiac muscle contains 150 times less catalase and nearly four times less superoxide dismutase than liver. (jci.org)
Dismutase2
- Catalase and Superoxide Dismutase of Root-Colonizing Saprophytic Fluorescent Pseudomonads. (epa.gov)
- The protective potential was carried on superoxide dismutase (SOD), catalase and peroxidases. (degruyter.com)
Levels1
Result1
- The temperature in our experiment was not very high which didn't result in denaturation of peroxidase. (ipl.org)
Mycobacterium3
- The Mycobacterium tuberculosis catalase-peroxidase is a multifunctional heme-dependent enzyme that activates the core anti-tuberculosis drug isoniazid. (nih.gov)
- Catalase-peroxidases, also called KatGs, have raised considerable interest due to their role in the activation of isoniazid, an anti-tubercular pro-drug for Mycobacterium tuberculosis. (auburn.edu)
- Strikingly, in this dissertation, we report the dramatic stimulation of the catalase activity of KatG from Mycobacterium tuberculosis (MtKatG) by up to 14-fold, in the presence of several common peroxidatic electron donors. (auburn.edu)
Stimulated catalase activity2
- During stimulated catalase activity, very little of the donor (0.008 eq. (auburn.edu)
- intermediate dominated during electron donor-stimulated catalase activity of MtKatG, and this intermediate converted directly to the ferric state upon depletion of H2O2. (auburn.edu)
Glutathione reductase3
- To this end, many assay methods have been developed for the detection of ROSrelated or ROS-derived substances such as superoxide anion, superoxide dismutase, glutathione, glutathione reductase, glutathione peroxidase, DNA lesions, 8-oxoguanine, 8-nitroguanosine, and protein carbonyl. (dojindo.com)
- Thiobarbituric acid reactive substances, reduced glutathione, Glutathione peroxidase Glutathione reductase, Superoxide dismutase and Catalase were also estimated to evaluate anti-oxidant status. (ijpsonline.com)
- Administration of a high-fat diet for 40 successive d significantly increased bad cholesterol and glucose levels, decreased good cholesterol levels and also thiobarbituric acid reactive substances level was significantly higher and whereas, reduced Glutathione, Glutathione peroxidise, Glutathione reductase, Superoxide dismutase and Catalase were significantly lower in disease control animals. (ijpsonline.com)
KatG3
- Catalase-peroxidase (EC 1.11.1.21, katG (gene)) is an enzyme with systematic name donor:hydrogen-peroxide oxidoreductase. (wikipedia.org)
- In fact, it has been established that the common peroxidatic electron donor o-dianisidine does inhibit the catalase activity of KatG from Escherichia coli at pH 7.5. (auburn.edu)
- Clearly, the catalase mechanism and the interrelationship between the catalase and peroxidase functions of KatG are much more complex than has been previously appreciated, and pH plays an important factor in both activities. (auburn.edu)
Ascorbate peroxidase2
- ascorbate peroxidase) and as well as regulating the key stress-responsive genes (OsSOD1, OsPOD1, OsCAT2, OsAPX1), thereby reinstate cellular membrane integrity and confer tolerance to ultrastructural damages under Cd-induced phytotoxicity. (bvsalud.org)
- Hydrogen peroxide (H2O2), membrane damage, ascorbate peroxidase (AP), catalase (CAT), proline and calcium increased in plants under drought conditions. (deepdyve.com)
Enzyme13
- This enzyme catalyses the following chemical reaction donor + H2O2 ⇌ oxidized donor + 2 H2O 2 H2O2 ⇌ O2 + 2 H2O This enzyme is a strong catalase with H2O2 as donor which releases O2. (wikipedia.org)
- Numerous studies have been undertaken to elucidate the enzyme-dependent mechanism of isoniazid activation, and it is well documented that mutations that reduce activity or inactivate the catalase-peroxidase lead to increased levels of isoniazid resistance in M. tuberculosis. (nih.gov)
- In order to provide a more accurate model of the three-dimensional structure of the M. tuberculosis catalase-peroxidase, we have crystallized the enzyme and now report its crystal structure refined to 2.4-A resolution. (nih.gov)
- Conversely, catalase activity can sustain high concentrations of H2O2 without the enzyme inactivation. (auburn.edu)
- Bifunctional enzyme with both catalase and broad- spectrum peroxidase activity. (ecmdb.ca)
- Turnip Peroxidase is the enzyme used in this lab and is derived from the vegetable. (ipl.org)
- The enzyme of turnip peroxidase is added in the equation to catalyze the oxidation. (ipl.org)
- Throughout this experiment, we conducted a study on peroxidase, which is an enzyme. (ipl.org)
- Temperature can have a big impact on the effectiveness of the peroxidase enzyme, which helps convert hydrogen peroxide into oxygen and water. (all-science-fair-projects.com)
- The hypothesis is that the peroxidase enzyme will become ineffective as a catalyst at high temperatures. (all-science-fair-projects.com)
- This science project is interesting because it explores how temperature affects the peroxidase enzyme, which is an important enzyme found in almost all living organisms. (all-science-fair-projects.com)
- Discover how temperature affects the enzyme Catalase and how it helps convert hydrogen peroxide into oxygen and water. (all-science-fair-projects.com)
- Let's explore how the enzyme catalase found in liver and potato affects the reaction between it and hydrogen peroxide! (all-science-fair-projects.com)
Heme1
- Zymomonas mobilis ZmCytC as a peroxidase bearing three heme c -binding motifs was investigated with Δ ZmcytC constructed. (karger.com)
Dismutases2
- In order to protect against the deleterious effects of ROS, a well-developed antioxidant system exists in the lung, which includes superoxide dismutases (SODs), catalase, and glutathione-dependent enzymes like glutathione peroxidase (GPx) 3 . (ersjournals.com)
- ROS scavenging enzymes include superoxide dismutases (SODs) and peroxidases such as catalases. (umd.edu)
Intracellular1
- 3HK-induced cell lysis was significantly attenuated by administration of catalase after termination of 3HK exposure and was abolished when intracellular peroxidase activity was elevated by pretreatment of cultures with horseradish peroxidase. (nih.gov)
Cytochrome1
- Peroxidase activity, which was detected in membrane fractions with ubiquinol-1 as a substrate but not with reduced horse heart cytochrome c , was almost abolished in Δ ZmcytC . (karger.com)
Protein1
Escherichia1
- 12. Cytotoxicity of lawsone and cytoprotective activity of antioxidants in catalase mutant Escherichia coli. (nih.gov)
Hydrogen2
- SOD converts superoxide anions to hydrogen peroxide and catalase converts hydrogen peroxide to water and oxygen 5 . (ersjournals.com)
- Hydrogen peroxide is reduced to water by catalase or glutathione peroxidase. (dojindo.com)
SODs1
- SODs have been characterized in P. marinus, but peroxidases have not been detected. (umd.edu)
Mitochondrial1
- Changes of antioxidant enzymes were examined using immunohistochemistry for cytoplasmic superoxide dismutase 1 (SOD1), mitochondrial (SOD2), catalase (CAT), and glutathione peroxidase (GPX). (spandidos-publications.com)
Oxidative stress2
- TiO2 NPs induced a strong oxidative stress in both glial cell lines by mediating changes in the cellular redox state and lipid peroxidation associated with a rise in the expression of glutathione peroxidase, catalase, and superoxide dismutase 2. (nih.gov)
- Catalase gene rs1001179 polymorphism and oxidative stress in patients with chronic hepatitis C and ulcerative colitis]. (cdc.gov)
Antioxidants1
- The antioxidants catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPx) were measured using chemical kinetic reactions under spectrophotometry. (ersjournals.com)
Nanoparticles2
- 7. Direct evidence for catalase and peroxidase activities of ferritin-platinum nanoparticles. (nih.gov)
- 9. SOD/catalase mimetic platinum nanoparticles inhibit heat-induced apoptosis in human lymphoma U937 and HH cells. (nih.gov)
Gene1
Temperature1
- The temperature in our experiment was not very high which didn't result in denaturation of peroxidase. (ipl.org)
Reaction1
- However, the peroxidase reaction is completed by the subsequent reduction of compound I to compound II (i.e. (auburn.edu)
Found1
- Results from the present study suggest that, while lacking catalase, P. marinus has ascorbate dependent peroxidases usually found in plants. (umd.edu)
Study1
- A recent study has identified decreased catalase and increased Mn SOD expressions in resected nonsmall cell lung carcinoma (NSCLC) 9 . (ersjournals.com)
Present1
- The various enzymes present in honey are glucose oxidase, sucrose diastase, catalase, and acid phosphatase [ 14 - 16 ]. (hindawi.com)