Caspase signaling pathway clone cells. Medical search. Frequent questions

Active caspases can then cleave many proteins, including poly-ADP ribose polymerase (PARP), other caspases and other protein substrates en masse. (bdbiosciences.com)
Reserpine can be an antihypertensive and antipsychotic medical medication that also inhibits multidrug level of resistance proteins 1 (MDR1).15-17 MDR1 or P-glycoprotein, Sesamin (Fagarol) is an associate from the ATP-binding cassette (ABC) transporter family that actively exports structurally unrelated substrates away of cells, to safeguard them from possible toxicities presumably. (kentlandsinitiative.org)
Adhesion complexes (i.e., focal adhesions and focal complexes) and cell-cell contacts are specialized structures that harbor a large number of cytoskeletal proteins and one of the highest concentrations of signaling molecules in cells ( Ruoslahti and Obrink, 1996 ). (rupress.org)
3 Pro-apoptotic proteins, such as cytochrome c and second mitochondria-derived activator of caspase (SMAC), are released from mitochondria into the cytosol where they participate in the activation of execution caspases. (nature.com)
Importantly, the mitochondrial association of n-cofilin and ADF had no impact on apoptosis signaling because ablation of either n-cofilin, ADF, or both proteins together did not change cell death progression. (nature.com)
Protein and gene expression of apoptotic signaling pathway-related proteins such as caspase-3, cytochrome c, and PARP were determined to provide evidence for the mechanisms of action of Tan IIA in the treatment of psoriasis. (hindawi.com)
Autophagy represents a caspase-independent process characterized by accumulation of autophagosomes in the cytoplasm and their subsequent fusion with lysosomes for degradation of unused or misfolded proteins or damaged structures such as ribosomes (ribophagy) and mitochondria (mitophagy). (aging-us.com)
2002). Akt blocks apoptotic stimuli by inactivating pro-apoptotic proteins such as for example Poor, caspase-9, and glycogen synthase kinase-3. (biomedigs.org)
Recent advances in understanding the molecular events underlying hypohidrotic ectodermal dysplasia (HED) caused by mutations of the genes encoding proteins of the tumor necrosis factor α (TNFα)-related signaling pathway have been presented. (springer.com)
These proteins are involved in signal transduction from ectoderm to mesenchyme during development of the fetus and are indispensable for the differentiation of ectoderm-derived structures such as eccrine sweat glands, teeth, hair, skin, and/or nails. (springer.com)
Novel data were reviewed and discussed on the structure and functions of the components of TNFα-related signaling pathway, the consequences of mutations of the genes encoding these proteins, and the prospect for further investigations, which might elucidate the origin of HED. (springer.com)
Within the last 10 years significant progress has been made in understanding the pathogenesis of HED and this is mainly due to the discovery of unknown proteins and the elucidation of their function in signal transduction via the TNFα-related pathway. (springer.com)
Because soybean Kunitz-type trypsin inhibitor has been found to induce apoptotic death of human being leukemia Jurkat cells (17) anti-leukemia activity of B. multicinctus protease inhibitor-like proteins is definitely therefore examined. (ecolowood.com)
The contributors describe strategies for identifying and evaluating the biochemical and morphological changes that occur during apoptosis, autophagy, necrosis, and other forms of cell death, as well as for pinpointing the genes and proteins that may be involved. (cshlpress.com)
In this respect, although obviously lacking a brain with a multicellular neuronal network, simple unicellular yeast are of great value to investigate the effects of overexpression and single point-mutations in genes encoding key proteins on cell physiology and on the function of conserved signaling cascades. (microbialcell.com)
Intracellular psoriasin has been found to interact with various signalling proteins, including the nuclear factor (NF)-kB regulator Jab1, while secreted psoriasin functions as an antimicrobial peptide and has the ability to induce chemotaxis of neutrophils and T cells (6). (medicaljournals.se)
Using a chemo-genetic approach, we identify BET bromodomain containing proteins BRD2 and BRD4 as central regulators of NK cell functions, including direct cytokine secretion, NK cell contact-dependent inflammatory cytokine secretion from monocytes as well as NK cell cytolytic functions. (frontiersin.org)

The robotic group had less intraoperative blood loss (p = 0.04), less postoperative abdominal drainage (p = 0.02), lower postoperative white blood cell counts (p = 0.024) and C-reactive protein levels (p = 0.017), and shorter catheter removal time when compared to the laparoscopic group (p = 0.003). (bvsalud.org)
Receptor-interacting protein kinase 1 (RIPK1) is a key component of the tumor necrosis factor (TNF) receptor signaling complex that regulates both pro- and anti-apoptotic signaling. (mdpi.com)
In this study, we found that RING finger protein 4 (RNF4), a RING finger E3 ubiquitin ligase, is required for the RIPK1 autophosphorylation and subsequent cell death. (mdpi.com)
The cellular FLICE-inhibitory protein (c-FLIP) is a modulator of death receptor-mediated apoptosis and plays a major role in T- and B-cell homeostasis. (ashpublications.org)
Visible Abstract Open up in another window Intro Inhibitor of apoptosis (IAP) protein regulate cell success in response to many stimuli. (kentlandsinitiative.org)
Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. (thermofisher.com)
The coding region of IL-33 mature protein was cloned into the prokaryotic expression vector pET-44. (hindawi.com)
The precursor protein (pre-IL-33) was cleaved by caspase-1 into a mature 18-kDa protein (IL-33) in in vitro experiments using a recombinant protein [ 2 ]. (hindawi.com)
In this study, the cloning and expression of mouse IL-33 gene in Escherichia coli (E. coli ), purification of recombinant protein, and generation of polyclonal antibody against IL-33 are described. (hindawi.com)
Injury-induced cytochrome c -specific cleavage of caspase-9 followed by activation of caspase-3 in mature brain correlated with marked increases in Apaf-1 and caspase-3 mRNA and protein expression. (jneurosci.org)
Full-length recombinant human caspase-3 protein was used as immunogen. (thermofisher.com)
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. (thermofisher.com)
This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. (thermofisher.com)
It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. (thermofisher.com)
Humanin (HN) can be an anti-apoptotic peptide that suppresses neuronal cell death induced by Alzheimer's disease, prion protein fragments, and serum deprivation. (biomedigs.org)
2004) reported that HN BMS-790052 binds to a human being G BMS-790052 protein-coupled formylpeptide receptor-like-1 BMS-790052 (FPRL1), which induces chemotaxis of mononuclear phagocytes and prevents A-induced apoptotic cell loss of life by competitively inhibiting A binding to FPRL1. (biomedigs.org)
The simultaneous expression of the three genes has been demonstrated at RNA level by RT-PCR and at protein level by immunofluorescence, fluorescence activated cell sorting (FACS) and western blotting. (unimib.it)
We observed that the WNV-Cp protein is a pathogenic protein, which drives apoptosis in vitro through the mitochodrial/caspase-9 pathway. (cdc.gov)
We also observed that expression of Cp protein in mouse muscle resulted in apoptosis and inflammation of muscle cells. (cdc.gov)
For a caspase-9-specific test, 5 μg of pcWNV-Cp-DJY or pcWNV-CpWT was cotransfected with a dominant negative caspase-9 (DN caspase-9) construct, and cleavage of procaspase-9 protein was determined by Western blot analysis with antihuman caspase-9 antibody (MBL, Nagoya, Japan). (cdc.gov)
This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. (cancerindex.org)
This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. (cancerindex.org)
TotalSeq™-D antibodies are compatible with Mission Bio's Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein. (biolegend.com)
TotalSeq™-D reagents are designed to profile protein expression at single cell level. (biolegend.com)
Three protease inhibitor-like protein genes have been cloned from Bungarus multicinctus genome in our laboratory (16). (ecolowood.com)
With this study human being leukemia U937 cells were treated with B. multicinctus protease inhibitor like protein-1 (PILP-1). (ecolowood.com)
Collectively our data elucidate a novel ADAM17/Lyn/Akt signaling pathway in keeping the viability of leukemia cells and suggest a strategy in improving leukemia therapy through suppression of ADAM17 protein expression. (ecolowood.com)
2004. Activator protein 2alpha associates with adenomatous polyposis coli/beta-catenin and Inhibits beta-catenin/T-cell factor transcriptional activity in colorectal cancer cells. . (oregonstate.edu)
FTS acts as a functional Ras antagonist in cells, affecting Ras-membrane interactions by dislodging the protein from its anchorage domains, facilitating its degradation and, thus, reducing cellular active Ras content [ 8 , 9 ]. (oncotarget.com)
We show that a member of the FK506-binding protein family, FKBP8, is an essential antagonist of SHH signaling in CNS development. (silverchair.com)
The differentiation-induced psoriasin expression was found to be mediated by the protein kinase C pathway. (medicaljournals.se)

Biologically, downregulation of SFN suppressed tumor cell proliferation, epithelial-mesenchymal transition (EMT), invasion, and migration in vitro and tumor growth in vivo. (bvsalud.org)
However, overexpression of SFN promoted cell proliferation, EMT, invasion, and migration in vitro and tumor growth in vivo. (bvsalud.org)
DHX15 silencing greatly inhibited leukemia cell proliferation and induced cell apoptosis and G1-phase arrest. (oncotarget.com)
These receptors are responsible for activating key pathways in cancer cells that lead to their proliferation and survival. (wisc.edu)
In 2008 our lab was the first to report that tumor cells that acquired resistance to cetuximab exhibited dramatic up regulation of the EGFR, secondary to loss of ubiquitination, which led to enhanced signals to other EGFR family members (HER and HER3) that promoted proliferation and survival. (wisc.edu)
Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
We found that JOA at lower concentration significantly inhibited the proliferation of CML cells expressing mutant BCR-ABL (T315I mutation included) and wild-type BCR-ABL, which was due to that JOA induced the cell differentiation and the cell cycle arrest at G0/G1 phase. (jcancer.org)
BCR-ABL oncogene encodes a BCR-ABL tyrosine kinase (TK), which triggers different downstream targets including c-Myc, STAT5 and CrkL, which participated in the control of cell differentiation, proliferation, migration and other cellular behaviors [ 3 - 5 ]. (jcancer.org)
Transforming growth factor-β (TGF-β) signaling pathway is a multifunctional cytokine and plays important roles in regulating cell proliferation, cell cycle, differentiation, migration, and apoptosis in a broad spectrum of tissues [ 12 ]. (degruyter.com)
2003. Promotion versus suppression of rat colon carcinogenesis by chlorophyllin and chlorophyll: modulation of apoptosis, cell proliferation, and beta-catenin/Tcf signaling. . (oregonstate.edu)
Epidermal growth factor receptor blockade with C225 modulates proliferation, apoptosis, and radiosensitivity in squamous cell carcinomas of the head and neck. (natprobiotech.com)
We have shown previously that extracellular psoriasin binds to the receptor for advanced glycation end products (RAGE), promoting endothelial cell proliferation (8). (medicaljournals.se)

Caspases-9, -8 and -3 have been implicated in apoptosis-caspase-9 in the mitochondrial pathway, caspase-8 in the Fas/CD95 pathway, and caspase-3 more downstream, activated by multiple pathways. (bdbiosciences.com)

Monoclonal antibody therapy has been developed over the past 30 years to target receptor tyrosine kinases (RTKs), which sit on the outer membrane of cancerous cells. (wisc.edu)
Gene amplification, overexpression and hyperactivity of the epidermal growth factor receptor (EGFR) is frequently observed in non-small cell lung cancer (NSCLC), head and neck cancer (HNSCC), breast and colorectal cancer (CRC). (wisc.edu)
Mouse anti Human CD142 antibody, clone TF9-10H10 recognizes human CD142, also known as Tissue Factor, is the membrane receptor for coagulation factors VII and VIIa and is the cell surface initiator of coagulation. (bio-rad-antibodies.com)
In TNF receptor (TNFR) superfamily signaling, they are essential to activate the canonical NF-B MAPKs and pathway. (kentlandsinitiative.org)
Rational design of chimeric antigen receptor T (CAR-T) cells based on the recognition of antigenic epitopes capable of evoking the most potent CAR activation is an important objective in optimizing immune therapy. (nature.com)
Most of all, the putative HN receptor for the cell membrane is not cloned or characterized (Hashimoto et al. (biomedigs.org)
2005) suggested how the HN receptor might participate in a tyrosine kinase receptor family members since a tyrosine kinase inhibitor (genistein) attenuates the neuroprotective activity of HNG against A toxicity in F11 neuronal crossbreed cells. (biomedigs.org)
Since epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors, including gefitinib (GEF) have been reported to induce the apoptosis of several cancer cell lines, in the present study, we examined whether the cytotoxic effects of GEF are further enhanced under amino acid starvation (AAS) culture conditions. (spandidos-publications.com)
TGF-β signaling is initiated when the ligand binds to type II serine/threonine kinase receptor (TGFBR2), which then phosphorylates and activates type I serine/threonine kinase receptor (TGFBR1) [ 13 ]. (degruyter.com)
It had been recommended that bikunin and tissues aspect pathway inhibitor-2 exerted their biology actions through a cell surface area receptor-mediated procedure (3 4 Furthermore tissue aspect pathway inhibitor-2 elicits pro-apoptotic signaling pathway in the individual fibrosarcoma cell series (8). (ecolowood.com)
The degree of chronic lymphocytic leukemia (CLL) B-cell antigen receptor (BCR) binding to myosin exposed apoptotic cells (MEACs) correlates with worse patient outcomes suggesting a link to disease activity. (ecologicalsgardens.com)
Paclitaxel enhances the effects of the anti-epidermal growth factor receptor monoclonal antibody ImClone C225 in mice with metastatic human bladder transitional cell carcinoma. (natprobiotech.com)

BD Biosciences also offers a range of tools for caspase activity assays from individual fluorogenic peptide substrates and inhibitors, to kits, to ready-to-use assay plates. (bdbiosciences.com)
The individual synthetic tetrapeptide substrates, together with the caspase inhibitors and active caspase enzymes, offer flexibility in the experimental design of a caspase activity assay. (bdbiosciences.com)
Despite a large number of promising inhibitors for Ras/Raf/MEK/ERK pathway, recent works show that cancer cells often develop an autophagy-dependent resistance to inhibitors of Ras pathway [ 16 ]. (aging-us.com)
IL10RB Open up in another window Amount 1 The consequences of HNG as well as the PI3K/Akt inhibitors wortmannin and Akti-1/2 on OGD-induced cell loss of life in principal cortical neurons. (biomedigs.org)
JOA displayed the activity of inhibiting the BCR-ABL and promoted differentiation of not only imatinib -sensitive but also imatinib -resistant cells with BCR-ABL mutation, which could become a potent lead compound to overcome the imatinib -resistant induced by inhibitors of BCR-ABL tyrosine kinase in CML therapy. (jcancer.org)
Many Kunitz-type protease inhibitors including bikunin hepatocyte development aspect activator inhibitor-2 and tissues aspect pathway inhibitor-2 are located to suppress tumor invasion and metastasis (4 -7). (ecolowood.com)
Consistent with this result A66 or AG490 inhibitors did not prevent MEACs from increasing CLL cell viability (Figure 6e-f = 0.0006 and = 0.0002 respectively). (ecologicalsgardens.com)
Thus inhibitors of BTK or PI3Kδ but not PI3Kα or JAKs block MEAC-induced increase in CLL cell viability supporting the hypothesis that BCR signaling molecules are involved in Isradipine this effect. (ecologicalsgardens.com)
A role for the BCR in this process is implied by blocking this effect with inhibitors of BTK and PI3Kδ key molecules in the BCR signaling pathway but not with inhibitors of PI3Kα and JAKs (Figure 6 Supplementary Tables S2-S6). (ecologicalsgardens.com)

The overall goal of this research is to identify these mechanisms of resistance and to target specific pathways to increase the efficacy of molecular targeting agents. (wisc.edu)
4 Although the mechanisms of caspase activation in the cytosol are well characterized, the steps that control cytochrome c release from mitochondria and thereby activate the apoptosis program have remained largely elusive. (nature.com)
The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
Cancer cells exploit a variety of protective mechanisms that allow them to acquire selective advantage and proliferate under unfavorable conditions. (aging-us.com)
One of the mechanisms of high basal autophagy activity in cancer Ras-expressing cells might be associated with high PP2A phosphatase activity targeting directly ULK1-Ser757 that could explain apparent contradiction: maintenance of high mTORC1 functions and high autophagic activity simultaneously [ 22 ]. (aging-us.com)
Cells use a number of mechanisms to sense and maintain their homeostatic levels. (spandidos-publications.com)
The mechanisms by which cell death occurs are genetically encoded and carefully controlled. (cshlpress.com)
Chapters are additionally devoted to cell death signaling mechanisms in plants and lower organisms, as well as the evolution of those mechanisms and the influence of pathogens that seek to evade them. (cshlpress.com)
This pattern is largely generated through cell-cell signaling mechanisms involving locally secreted signaling molecules( Jessell, 2000 ). (silverchair.com)

Caspases are activated upon cleavage at aspartate residues during the earliest stages of apoptosis. (bdbiosciences.com)
All are based on the use of synthetic tetrapeptide substrates that are designed such that proteolytic cleavage by active human or mouse caspases results in release of a fluorophore or chromophore. (bdbiosciences.com)
As such it will recognize pro Caspase-3 and the large subunit cleavage fragment. (thermofisher.com)
First because both intrinsic and extrinsic pathways of apoptosis lead to cleavage of intracellular myosin exposure on the cell surface (Figure 2) and production of MEACs (Figure 1) with caspase-3 activation in principle any cell type can form MEACs including CLL cells themselves (Figure 3). (ecologicalsgardens.com)

In contrast, the restoration of DHX15 expression rescued cell viability and reduced cell apoptosis. (oncotarget.com)
Methods for detecting apoptosis or dead cells (viability) by cell preparation type. (bdbiosciences.com)
After 60 min of OGD and 24 h reperfusion, cell viability reduced to 52.9 0.93% from the control. (biomedigs.org)
Cell BMS-790052 viability was evaluated with the MTS assay at 24 h of reperfusion. (biomedigs.org)
The observed difference in cell viability in hypoxia was associated with the increased number of apoptotic cells in dnHIF-1α clones (Hoechst 33258 staining). (elsevierpure.com)
Functionally co-culture of MEACs with CLL cells regardless of immunoglobulin heavy chain variable region gene mutation status improved leukemic cell viability. (ecologicalsgardens.com)
Based on inhibitor studies this improved viability involved BCR signaling molecules. (ecologicalsgardens.com)
These results support the hypothesis that stimulation of CLL cells with antigen such as those on MEACs promotes CLL cell viability Isradipine which in turn could lead to progression to worse disease. (ecologicalsgardens.com)
Figure 5 MEACs associate with CLL cells and enhance CLL cell viability. (ecologicalsgardens.com)
Indeed MEAC co-culture with CLL cells consistently improved CLL cell viability (Figures 5-6 Supplementary Table S1). (ecologicalsgardens.com)
This indispensable laboratory resource will enable all cell and developmental biologists, from the graduate level upward, to confidently carry out and comprehend a wide array of cell viability assays. (cshlpress.com)

In this study, we found that SFN was upregulated in HCC cell lines and tissues and was positively associated with tumor size, poor differentiation, Tumor Node Metastasis (TNM) stage, and vascular invasion. (bvsalud.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Although the LAT family plays important roles in the development and function of normal tissues, they are frequently increased in cancer cells ( 3 ). (spandidos-publications.com)
In this study, we investigated the role of autophagy and apoptosis in Newcastle disease virus (NDV)-infected chicken cells and tissues. (immune-source.com)
In bottom line, we confirmed that autophagy brought about by genotype VII NDV infections was important for virus-like replication, NDV-induced apoptosis, and cell survival in buy 480-11-5 chicken cells and tissues. (immune-source.com)
Although it is expressed broadly, FKBP8 is required to antagonize SHH signaling primarily in neural tissues, suggesting that hedgehog signal transduction is subject to cell-type specific modulation during mammalian development. (silverchair.com)
DAMPs, damage-associated tissues and the molecular target structures and pathways. (cdc.gov)

This can provide Ras-expressing tumor cells additional selective advantages under stress or damage. (aging-us.com)
Objectives Mesenchymal stem cells (MSCs) play an important role in the development and growth of tumor cells. (immune-source.com)
Cell cycle analysis showed an accumulation of tumor cells predominantly in 511296-88-1 manufacture G0/G1 phase with an increase in concentration of TD-MSCs, which was confirmed by increased mRNA expression of cell cycle negative regulator p21. (immune-source.com)
Apoptosis of tumor cells increased significantly as concentration of cocultured TD-MSCs increased. (immune-source.com)
Cancer gene therapy approaches include the direct killing of tumor cells by injecting a therapeutic gene into the tumor cell or employing vaccine strategies to deliver an immunomodulatory gene that stimulates the immune system to recognize tumor antigens [ 1 ]. (biomedcentral.com)
When expressed in tumors, TK converts the non-toxic precursor GCV into GCV- 3-phosphate, a toxic substance that kills tumor cells. (biomedcentral.com)

Apoptosis is induced through the mitochondrial pathway resulting in caspase-9 activation and downstream caspase-3 activation. (cdc.gov)

Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
Transgenic (TG) and wild type (WT) NIH3T3 were tested in a TNF- mediated cytotoxicity assay and caspase activation assay. (unimib.it)
The localization pattern of capsid expression was analyzed by immunofluorescent assay in HeLa, 293-T, RD, or SH-SY5Y cells by using anti-His tag antibody as described ( 6 ). (cdc.gov)
In vitro apoptosis in individual cells was determined by terminal desoxyriboxyl-desoxyriboxyl transferase-mediated DVTP nick-end labeling (TUNEL) assay with the In Situ Cell Death Assay Kit (Roche Diagnostic Corp., Indianapolis, IN) and visualized by fluorescent microscopy. (cdc.gov)

Chronic myeloid leukemia (CML) results from BCR-ABL oncogene, which blocks CML cells differentiation and protects these cells from apoptosis. (jcancer.org)
Mechanistically, the cell differentiation mediated by JOA may be originated from the inhibition of BCR-ABL/c-MYC signaling in CML cells expressing wild-type BCR-ABL and BCR-ABL-T315I. (jcancer.org)
With the exception of EDA1 and NEMO , both localized on the X chromosome, all other genes encoding components of the TNFα-related signaling pathway involved in differentiation of skin appendages, are localized on the autosomes (Table 1 ). (springer.com)
The purpose of this report is to review current literature on the structure and function of components of the TNFα-related signaling pathway, to present a novel approach to their contribution in the differentiation of skin appendages and to discuss the role of mutations of genes encoding components of this pathway in the origin of HED. (springer.com)
The signaling via the TNFα-related pathway, which is critical for differentiation of skin appendages, has been elucidated mainly due to the investigations performed in mice (Mikkola 2009 ). (springer.com)
The Ras family of small GTPases transmits extracellular signals that regulate cell growth, differentiation, motility and death. (oncotarget.com)
The Ras family of small GTPases transmits signals initiated by cell surface receptors, to regulate several cellular processes such as cell growth, differentiation, motility and death [ 1 ]. (oncotarget.com)
Role of p27(Kip1) in human intestinal cell differentiation. (natprobiotech.com)
In psoriasis, the differentiation process of the epidermal cells is disturbed. (medicaljournals.se)
In a spectrum of conditions of dysregulated keratinocyte differentiation, we have previously demonstrated strong psoriasin expression in differentiated squamous cell carcinoma and a lack of expression in undifferentiated basalioma (9). (medicaljournals.se)

Several downstream targets of the NF-kB pathway were also down-regulated, and apoptosis-associated genes CASP3 and PARP were activated. (oncotarget.com)
Mitochondrial regulation of apoptosis further downstream was investigated, showing changes in the mitochondrial membrane potential, cytochrome c release into the cytoplasm, and enhanced activation of cleaved caspase-3 and Poly ADP-ribose polymerase (PARP). (hindawi.com)
The downstream signal transduction toward Eda-A1 involves the canonical Wnt/β-catenin pathway (Clevers 2006 ) and requires lymphoid enhancer-binding factor-1 (Lef-1) for activation of the Ed1 ( Ta ) expression (Durmowicz et al. (springer.com)

Mouse anti Human CD142 antibody, clone TF9-10H10 recognizes an epitope within the extracellular domain, epitope locus I. It recognizes both the reduced and native non-reduced human and primate tissue factors. (bio-rad-antibodies.com)
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
The following product was used in this experiment: Active/Pro-Caspase 3 Monoclonal Antibody (31A1067) from Thermo Fisher Scientific, catalog # MA1-91637, RRID AB_1954999. (thermofisher.com)
Western Blot analysis of (A) RAW264.7 and (B) NIH-3T3 cell lysate using Active/Pro-Caspase 3 Monoclonal Antibody (31A1067) (Product # MA1-91637). (thermofisher.com)
The antibody recognizes an epitope in the large domain subunit of Caspase-3. (thermofisher.com)
Since its discovery in 2000, initial in vitro studies have found that the engagement of Siglec-8 with a monoclonal antibody or with selective polyvalent sialoglycan ligands induced the cell death of eosinophils and inhibited mast cell degranulation. (mdpi.com)
Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse eosinophils and mast cells confirmed the in vitro findings, and identified additional anti-inflammatory effects. (mdpi.com)
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. (biolegend.com)
The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. (biolegend.com)
Anti-caspase-3 antibody anti-caspase-8 antibody Ac-DEVD-p-nitroanilide Ac-LEHD-p-nitroanilide Z-IETD-fmk (caspase-8 inhibitor) Z-DEVD-fmk (caspase-3 inhibitor) and PP2 (Lyn inhibitor) were purchased from Calbiochem. (ecolowood.com)
Colorectal cancer cells are resistant to anti-EGFR monoclonal antibody through adapted autophagy. (natprobiotech.com)
Anti-EGFR antibody sensitizes colorectal cancer stem-like cells to Fluorouracil-induced apoptosis by affecting autophagy. (natprobiotech.com)

BD Biosciences tools use multiple methodologies including flow cytometry , bioimaging and microscopy (for live and fixed cell analysis) as well as ELISA , IHC , western blot and spectrofluorometry. (bdbiosciences.com)
During apoptosis, PS translocates to the outer leaflet of the plasma membrane and can be detected by flow cytometry and cell imaging through binding to fluorochrome-labeled annexin V when calcium is present. (bdbiosciences.com)
Functional characterization of high and low avidity GSW11-specific CD8 + T cells was conducted using flow cytometry and bulk RNA-seq. (bmj.com)
Cell apoptosis was analyzed by flow cytometry. (degruyter.com)

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. (origene.com)
The differences in the activation of TNF-a signaling pathway were analysed at molecular level by using an RT2 Profiler TM PCR array system in which the expression of 84 TNF-related pathway genes were evaluated by real-time PCR. (unimib.it)
Correspondence with low molecular weight chemicals that results in the priming of chemical-specific, Prof. Dr. Stefan F. Martin, Allergy Research skin-homing CD8+ Tc1/Tc17 and CD4+ Th1/Th17 cells. (cdc.gov)

Since natural IgM Abs can recognize MEACs (Figure 4) this is consistent with the rationale of apoptotic cell removal.39 It is also consistent with at least some CLL clones deriving from B cells that produce natural autoreactive Abs that are used as their surface receptors and Isradipine potentially as their secreted effector molecules. (ecologicalsgardens.com)
MW) chemicals interact with host cells and molecules and In our daily life, we are confronted with a plethora of natural influence biologic processes such as signal transduction. (cdc.gov)

Active caspase-9, in turn, activates executioner caspases-3 and -7. (jneurosci.org)
Oncogenic Ras induces the constitutive activation of Ras/Raf/MEK/ERK signaling pathway, which, in turn, activates other effector pathways, in particular, PI3K-mTOR signaling [ 17 ]. (aging-us.com)

2005). The PI3K/Akt pathway can be a central mediator in sign transduction pathways involved with cell development, cell success, and rate of metabolism (Brazil, Yang and Hemmings, 2004). (biomedigs.org)

Since intracellular annexin V is also exposed if the plasma membrane is compromised, a membrane-impermeant dye such as 7-AAD is commonly used to distinguish between apoptotic and dead cells to exclude the dead cells. (bdbiosciences.com)
These multiprotein complexes are capable of capturing and integrating many signals from the extracellular as well as intracellular environments ( Rosales and Juliano, 1995 ). (rupress.org)
Studies on intracellular signaling revealed that the degree of cytochrome c release, dissipation of mitochondrial membrane potentials, and caspase-9 activity were all more prominent in dnHIF-1α clones than in control IRPTC, pointing to the accelerated signaling of mitochondrial pathways. (elsevierpure.com)

It has been reported that RNF4 negatively regulates TNF-α-induced activation of the nuclear factor-κB (NF-κB) through downregulation of transforming growth factor β-activated kinase 1 (TAK1) activity, indicating the possibility that RNF4-mediated TAK1 suppression results in enhanced sensitivity to cell death. (mdpi.com)
Knockdown of DHX15 inhibited the nuclear translocation and activation of the NF-kB subunit P65 in leukemia cells. (oncotarget.com)
One of the most consistently observed characteristics of apoptosis is the activation of a series of cytosolic proteases called caspases. (bdbiosciences.com)
Transfection with paxillin Y31FY118F dominant-negative mutant in these cells inhibits ERK activation and restores apoptosis. (rupress.org)
Cytochrome c release, caspase-3 activation, and apoptotic chromatin condensation were unchanged in all mutant fibroblasts. (nature.com)
These results suggest that differential expression of Apaf-1 and caspase-3 genes may underlie regulation of apoptotic susceptibility during brain development, as well as after acute injury to mature brain, through the intrinsic pathway of caspase activation. (jneurosci.org)
Eldadah and Faden, 2000 ), we hypothesized that suppression of apoptotic capability during maturation of mammalian brain results from repression of genes involved in the caspase-3 activation pathway, and that injury-induced neuronal apoptosis in the mature brain results from reactivation of these genes. (jneurosci.org)
Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. (thermofisher.com)
The successful strategy based on the activation of cell death in Ras-expressing cells is associated with the suppression of kinases involved in Ras pathway. (aging-us.com)
Our earlier research proven that HNG exerts its neuroprotective results on cerebral ischemia damage by inhibiting the activation from the extracellular sign controlled kinase (ERK), indicating that HNG's protecting impact reaches least partly mediated from the tyrosine kinase Ras/MEK/ERK pathway (Xu et al. (biomedigs.org)
Akt also exerts anti-apoptotic results by activating endothelial nitric oxide synthase (eNOS) (Brazil, Yang and Hemmings, 2004) Provided the neuroprotective part from the PI3K/Akt pathway in cerebral ischemia, we hypothesized that HN's neuroprotective impact requires the activation from the PI3K/Akt pathway after cerebral I/R damage. (biomedigs.org)
Involved in type-I interferon-induced apoptosis characterized by a rapid and robust release of cytochrome C from the mitochondria and activation of BAX and caspases 2, 3, 6, 8 and 9 (PubMed:18330707, PubMed:27673746). (origene.com)
Both test showed a significant reduction in cytotoxicity (-26% at 24h, -44% at 48h) and caspase activation (-27% at 16h) in TG cells in comparison with WT cells. (unimib.it)
Besides, overexpression of miR-128-3p downregulated TGFBR2 to suppress the activation of the Smad signaling pathway. (degruyter.com)
Subsequently, caspase 8 is released from DISC and induces activation of caspase 3 and 7, proteolysis of cellular components, and apoptosis. (biolegend.com)
Interestingly, the combined treatment enhanced apoptotic cell death as indicated by increased sub-G1 cell population, increased Hoechst staining, activation of caspase 3, decrease in survivin expression and release of cytochrome c. (oncotarget.com)
Apoptotic signaling is initiated either through extrinsic or intrinsic stimulation, resulting in the activation of caspases [ 12 ]. (biomedcentral.com)
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1a induced cell death. (lu.se)
The resulting aggregate called death-inducing signaling complex (disc) performs CASP8 proteolytic activation. (lu.se)
Loss of FKBP8 causes ectopic and ligand-independent activation of the Shh pathway, leading to expansion of ventral cell fates in the posterior neural tube and suppression of eye development. (silverchair.com)
Their function is regulated by the activation of a number of activating and inhibitory receptors that bind to specific ligands expressed on the surface of target cells. (frontiersin.org)
Following activation, NK cells mediate killing of target cells through two major pathways that require direct contact between NK cells and their target cells ( 6 ). (frontiersin.org)

IL-33 can promote survival, adhesion, and cytokine production in human mast cells [ 11 ], but the mechanism behind its involvement in inflammatory conditions remains unclear. (hindawi.com)
We show that both BRD2 and BRD4 control inflammatory cytokine production in NK cells isolated from healthy volunteers and from rheumatoid arthritis patients. (frontiersin.org)
Given the important role of both cytokine-mediated inflammatory microenvironment and cytolytic NK cell activities in immune-oncology therapies, our findings present a compelling argument for further clinical investigation. (frontiersin.org)
In particular, NK cells mediate their cytolytic function through the engagement of activating receptors, such as NKG2D, DNAM-1, NKp30, NKp46, and NKp44 ( 3 , 4 ), or following pro-inflammatory cytokine stimulation ( 5 ). (frontiersin.org)

Single-cell transcriptome RNA sequencing coupled with functional T-cell proteomics analysis uncovers the top-upregulated genes that might be critical for the persistence of polyfunctional CAR-T cells in mice. (nature.com)
METHODS AND RESULTS: A triple cistronic vector, named pCX TRI-2A, harbouring hHO-1, hCD73 and hCD39 coding sequences, has been produced by cloning in frame the sequences of the three genes by using the F2A-based technology. (unimib.it)
This effect is more evident and further increased in TG cells over-expressing the three genes. (unimib.it)
The activity of the hCD39/hCD73 genes in TNF--treated TG cells maintained up-regulated the anti-inflammatory Tnfaip3 gene and the activity of hHO1 in TNF--treated TG cells up-regulated the anti-apoptotic Traf3 gene as compared to WT cells. (unimib.it)
The protective effect given by the simultaneous expression of hHO1, hCD73 and hCD39 genes against TNF-alpha toxicity is characterized by the up-regulation of specific genes inhibiting the NfkB pathway. (unimib.it)
Transcriptomics analyses showed pathways related to TCR signaling, cytotoxicity and oxidative phosphorylation were significantly enriched in Tet lo found in both regressing and progressing tumors compared with Tet hi , whereas genes related to DNA damage, apoptosis and autophagy were downregulated. (bmj.com)
HED embraces a genetically heterogeneous group of diseases and is due to mutations of several genes that encode components of the tumor necrosis factor α (TNFα)-related signaling pathway (Cluzeau et al. (springer.com)
We may conclude that CTX induces apoptosis and autophagy in HT-29 KRK cells through raising the expression of p21, p27 , and p57 genes, as well as ATG4A, LC3A , and BECN1 genes. (natprobiotech.com)
SHH signaling regulates dorsoventral patterning of the neural tube by repressing genes that are associated with dorsal and lateral fates and by activating genes associated with ventral fates (reviewed by Briscoe and Ericson, 2001 ). (silverchair.com)

Interestingly, mitochondrial association of ADF and n-cofilin during apoptosis was preceded by, and dependent on, actin that translocated by a yet unknown mechanism to mitochondria during cell death. (nature.com)
There was also no translocation of apoptosis-inducing factor (AIF) from mitochondria to the nucleus in apoptotic keratinocytes, indicating Tan IIA-induced apoptosis occurs mainly through the caspase pathway. (hindawi.com)
Treatment of control ERas cells with PD0325901 for 24 h results in mitochondria damage and apoptotic death of a part of cellular population. (aging-us.com)
Senescent cells are unable to form the autophagolysosomes and to remove the damaged mitochondria resulting in apoptotic death. (aging-us.com)

BD Biosciences carries a variety of reagents to measure caspases, particularly caspase-3. (bdbiosciences.com)
Cell lifestyle items were purchased from Invitrogen Unless buy 435-97-2 specific all the reagents were of analytical quality in any other case. (ecolowood.com)

Here we demonstrate that, during brain maturation, the potential of the intrinsic apoptotic pathway is progressively reduced and that such repression is associated with downregulation of apoptotic protease-activating factor-1 (Apaf-1) and caspase-3 gene expression. (jneurosci.org)
Furthermore, it is possible to say that reducing EGF and KRAS gene expression inhibits cancer cell growth. (natprobiotech.com)

The reciprocal functions of RIPK1 in TNF signaling are determined by the state of the posttranslational modifications (PTMs) of RIPK1. (mdpi.com)

Inhibition of cytotoxic effect of FASL on Jurkat cells in the presence of recombinant human FAS. (biolegend.com)
The binding of FASL to FAS induces conformational changes in FAS, leading to the recruitment of FADD and procaspase-8 and the assembly of the death-inducing signaling complex (DISC). (biolegend.com)
The interaction of FAS/FASL in immature dendritic cells (DCs) induces functional maturation. (biolegend.com)
In addition, FAS/FASL interaction increases the production of chemokines in NK-T cells, and FASL acts as a survival factor for human CD34 + cells and increases their colony formation. (biolegend.com)
ED 50 = 10 - 50 ng/ml, corresponding to a specific activity of 0.02 - 0.1 x 10 6 units/mg, as determined by inhibition of apoptotic cell death induced by FASL on Jurkat cells. (biolegend.com)
The apoptosis was transduced by multiple signaling pathways mediated by FasL and TNFR2 and mainly activated the mitochondrial control of apoptosis via Bid and Bim, which was rescued by silencing Bid or/and Bim . (biomedcentral.com)
However, BF + GCV only induced apoptosis via Fas/FasL signal pathway accompanied with increased P53 expression. (biomedcentral.com)
Fas/FasL) that results in caspase-dependent apoptosis. (frontiersin.org)

Additionally, mRNA expression of caspase 3 was upregulated with increased concentration of TD-MSCs. (immune-source.com)

Here, we generate dromedary camel nanobodies targeting B7-H3 and demonstrate that CAR-T cells, based on the nanobodies recognizing the IgC but not IgV domain, had potent antitumour activity against large tumors in female mice. (nature.com)
However, the successful application of these emerging cell-based therapies in solid tumors remains limited. (nature.com)
High avidity T cells (Tet hi ), if present, were only found in progressing PD-1 refractory tumors. (bmj.com)

The most potent nanobody-based CAR-T cells show inhibition of large tumor xenografts in mice with rigorous T-cell signaling and significant T-cell infiltration into the tumor. (nature.com)
We studied whether cellular senescence induced by HDAC inhibitor sodium butyrate in E1a+cHa-Ras -transformed rat embryo fibroblasts (ERas) and A549 human Ki-Ras mutated lung adenocarcinoma cells would enhance the tumor suppressor effect of MEK/ERK inhibition. (aging-us.com)
Senescent ERas cells do not develop cytoprotective autophagy upon inhibition of MEK/ERK pathway due to spatial dissociation of lysosomes and autophagosomes in the senescent cells. (aging-us.com)
Autophagic cell death was excluded as the inhibition of autophagy did not attenuate the cytotoxicity. (spandidos-publications.com)
Previously, we have shown that Ras inhibition by FTS induces autophagy, which partially protects cancer cells and may limit the use of FTS as an anti-cancer drug. (oncotarget.com)
Since FTS is a non toxic drug we hypothesized that FTS and chloroquine (an autophagy inhibitor) will synergize in cell growth inhibition and cell death. (oncotarget.com)
Thus, chloroquine treatment may promote FTS-mediated inhibition of tumor cell growth and may stimulate apoptotic cell death. (oncotarget.com)
BDNF/TrkB signaling protects HT-29 human colon cancer cells from EGFR inhibition. (natprobiotech.com)

miR-128-3p could inhibit apoptosis and inflammation by targeting TGFBR2 in LPS-induced HK2 cells, which might provide therapeutic strategy for the treatment of sepsis. (degruyter.com)
DN caspase-9 (provided courtesy of Emad S. Alnmeri, Thomas Jefferson University, Philadelphia, PA) has been reported to inhibit the caspase cascade ( 5 ). (cdc.gov)
This is supported by pharmacological targeting where the first-generation pan-BET bromodomain inhibitor JQ1(+) displays anti-inflammatory effects and inhibit tumor cell eradication, while the novel bivalent BET bromodomain inhibitor AZD5153, which shows differential activity towards BET family members, does not. (frontiersin.org)

2004). We lately demonstrated that HNG protects against cerebral ischemia/reperfusion (I/R) damage by inhibiting apoptotic cell loss of life, reducing infarct quantity, and reducing neurological deficits in mice (Xu et al. (biomedigs.org)

Here, we show that vinculin null (vin −/− ) cells and cells expressing a vinculin Y822F mutant have increased survival due to up-regulated activity of extracellular signal-regulated kinase (ERK). (rupress.org)
Macroautophagy (hereafter referred to as autophagy) plays an important role in cancer cells survival upon various conditions of intra- and extracellular stress. (aging-us.com)

This gene thus plays a role in both cell-cycle and epigenetic regulation. (cancerindex.org)
It was found that PILP-1-induced down-regulation of a disintegrin and metalloprotease 17 (ADAM17) led to inactivation of Lyn/Akt pathways. (ecolowood.com)
2003. Suppression of tumorigenesis in the Apc(min) mouse: down-regulation of beta-catenin signaling by a combination of tea plus sulindac. . (oregonstate.edu)

Tet lo demonstrated precursor exhausted or progenitor T cell phenotypes marked by higher expression of Tcf-1 and T-bet, and lower expression of the exhaustion markers CD39, PD-1 and Eomes compared with Tet hi , whereas Tet hi cells were terminally exhausted. (bmj.com)

The catabolic processes regulated by mTORC1 control autophagy via a number of effector regulatory pathways [ 18 , 19 ]. (aging-us.com)

In fact, 24 hours of TNF- treatment induced a cytotoxicity of about 27% and 20% in WT and TG cells respectively, while the addition of hemin and ATP decreased the cytotoxicity up to 16% in WT cells and markedly more in TG cell reaching only 5% of cytotoxicity. (unimib.it)
CONCLUSIONS: The simultaneous over-expression of hHO-1, hCD73 and hCD39 in murine cells is protective against human TNF-alpha mediated cytotoxicity and apoptosis. (unimib.it)
The addition of essential amino acids, but not non-essential amino acids to the cell culture medium resulted in the cancellation of this pronounced cytotoxicity. (spandidos-publications.com)

In the present study, we evaluated the role of the intrinsic pathway in neuronal apoptosis at different stages of rat brain development and during maturation of primary cortical neurons in vivo as well as in response to brain injury. (jneurosci.org)
The first-in-human testing of scFv-based B7-H3 CAR-T cells has shown antitumor response against anaplastic meningioma and childhood diffuse intrinsic pontine glioma without severe side effects 16 , 17 . (nature.com)

Mechanistically, overexpression of SFN activated the Wnt/ß-catenin pathway by promoting Glycogen synthase kinase-3 beta (GSK-3ß) phosphorylation, decreasing ß-catenin phosphorylation, promoting ß-catenin transport into the nucleus, and enhancing the expression of c-Myc, whereas depletion of SFN inhibited the Wnt/ß-catenin pathway. (bvsalud.org)
This clone was engineered to express the complete ORF with an expression tag. (origene.com)
The level of miR-128-3p was decreased, and TGFBR2 expression was increased in serum samples of sepsis patients and LPS-induced HK2 cells. (degruyter.com)
2004. The dietary phytochemical chlorophyllin alters E-cadherin and beta-catenin expression in human colon cancer cells. . (oregonstate.edu)

We report that the WNV-capsid (Cp) by itself induces rapid nuclear condensation and cell death in tissue culture. (cdc.gov)
Our results demonstrate that in HCT-116 and in Panc-1 cells, FTS induces autophagy, which can be inhibited by chloroquine. (oncotarget.com)

These proteases are responsible for the dismantling of cells during apoptosis. (nature.com)

In addition, TOPFlash/FOPFlash reporter assays showed that overexpression or downregulation of SFN obviously increased or decreased, respectively, the activity of the Wnt/ß-catenin pathway. (bvsalud.org)
With the addition of these new formats, more complex assays can be developed to look at apoptosis within heterogeneous cell subsets. (bdbiosciences.com)
Tetramer staining was performed to determine the frequency and avidity of CD8 + T cells targeting the tumor-specific epitope GSW11 and confirmed with tetramer competition assays. (bmj.com)

Our data show that suppression of MEK/ERK pathway in senescent cells provides a new strategy for elimination of Ras-expressing cells. (aging-us.com)
We propose that HIF-1 mediates cytoprotection against cisplatin injury in hypoxic renal tubular cells, by reducing the number of apoptotic cells through stabilization of mitochondrial membrane integrity and suppression of apoptosis signaling. (elsevierpure.com)

DISCUSSION MEAC binding to recombinant CLL mAbs in vitro correlated with shorter patient survival consistent with autoantigen stimulation being involved in the growth and evolution of the leukemic clone.14 MEACs may provide an abundant source of such antigens which are not likely limiting in vivo for several reasons. (ecologicalsgardens.com)

The neuroprotective part from the PI3K/Akt pathway in cerebral ischemia continues to be widely researched (Janelidze et. (biomedigs.org)
Activated Ras transmits signals through its interaction with multiple effectors, including mitogen activated kinase (MAPK), phosphoinositide-3-kinase (PI3K) and Ral-GEF [ 1 , 2 ]. (oncotarget.com)

Mutation of cell-cycle regulators biological and clinical implications for human neoplasia. (natprobiotech.com)

This leads to the loss of cellular structure and function, and ultimately results in cell death. (bdbiosciences.com)

Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. (thermofisher.com)

As cells become damaged or are no longer needed, they undergo apoptosis or programmed cell death, a normal physiological process that occurs during embryonic development and tissue homeostasis. (bdbiosciences.com)
Apoptosis controls the orderly death of damaged cells, whereas necrosis occurs as a result of tissue damage, causing the loss of both damaged and surrounding cells. (bdbiosciences.com)
In those cases, multiple aspects of apoptosis might need to be analyzed to confirm the mechanism of cell death. (bdbiosciences.com)
Radio-frequency dose-dependent apoptosis, necrosis and cell death monitored by annexin V-BD Horizon™ V450. (bdbiosciences.com)
Radio-frequency dose-dependent apoptosis, necrosis and cell death monitored by annexin V-BD Horizon™ V450 in pancreatic carcinoma cell lines treated with a low dose of cetuximab targeted gold nanoparticles. (bdbiosciences.com)
Consequently, several human diseases, including cancer and neurodegenerative disorders, are caused by defects in this highly regulated form of cell death. (nature.com)
Apoptosis is a genetically controlled cell death that was initially recognized for its role in development. (jneurosci.org)
Capsid gene delivery into the striatum of mouse brain or interskeletal muscle resulted in cell death and inflammation, likely through capsid-induced apoptosis in vivo. (cdc.gov)
The signaling pathways further induced apoptosis of U937 cells through the mitochondrion-mediated death pathway. (ecolowood.com)
Other biological effects not leading to apoptotic cell death have also been described. (biolegend.com)
In multicellular organisms, cell death is required for normal development, homeostasis, and the elimination of infected or injured cells. (cshlpress.com)
Perturbations that enhance or suppress cell death may lead to cancer, neurodegeneration, and inflammatory diseases. (cshlpress.com)
Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology provides a comprehensive update on the cell signaling that underlies the main cell death programs (apoptosis, pyroptosis, and necroptosis) and how this knowledge is driving the development of therapeutic drugs to treat some human diseases. (cshlpress.com)
They also examine the involvement of cell death programs in various pathologies and the therapeutic potential of inhibiting key pathway components. (cshlpress.com)
This volume is therefore an essential reference for cell and developmental biologists, cancer biologists, and all who wish to explore recent progress in our understanding of cell death programs. (cshlpress.com)
Regulated cell death, which is involved in nearly every aspect of animal development and physiology, can be challenging to study in the laboratory. (cshlpress.com)
There are multiple types of cell death, several interconnected signaling pathways can be activated, and the postmortem consequences may vary. (cshlpress.com)
Therefore, a combination of specialized techniques is often required to characterize and confirm cell death in the system of interest. (cshlpress.com)
This laboratory manual provides a comprehensive suite of step-by-step protocols for inducing, detecting, visualizing, characterizing, and quantifying cell death in a variety of systems. (cshlpress.com)
The authors also provide guidance on interpreting and presenting the results of cell death experiments, as well as advice on complementary procedures that may be required to confirm the results of a given experiment. (cshlpress.com)
Generation of the subunits requires association with the death-inducing signaling complex (disc), whereas additional processing is likely due to the autocatalytic activity of the activated protease. (lu.se)

Changes in the plasma membrane are one of the characteristics of the apoptotic process detected in living cells. (bdbiosciences.com)
The transport of serum amino acids into cells is an active process that is facilitated by plasma membrane-localized amino acid transporters. (spandidos-publications.com)
Rather they supply a large number Fam162a of other autoantigens that the apoptotic process makes available to immune receptors.4 6 42 Surface membrane exposed myosin is primarily serving as an indicator of the type of cell involved in the process. (ecologicalsgardens.com)

Electron microscopy demonstrated that the CAL 27 cells treated with GEF under AAS culture conditions exhibited swelling of the cytosol and organelles with an increased number of autophagosomes and autolysosomes, but without chromatin condensation and nuclear fragmentation. (spandidos-publications.com)

CD33 antigen can function as a sialic acid-dependent cell adhesion molecule and involved in negative selection of human self-regenerating hemetopoietic stem cells. (kentlandsinitiative.org)
This clone is cross reactive with non-human primate * Diagnosis of acute myelogenousnleukemia. (kentlandsinitiative.org)
Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)
Among these, Siglec-8 is a CD33-related family member selectively expressed on human mast cells and eosinophils, and at low levels on basophils. (mdpi.com)
FAS was initially purified and cloned from SKW6.4 cells and a cDNA library of human T cell lymphoma KT-3 cells. (biolegend.com)
Human FAS, amino acids (Arg17-Asn173) (Accession# NM_000043), was expressed in 293E cells. (biolegend.com)
Possible sources of these Abs could be B-1 cells which express CD5 in mice and in ~75% of human B-1 cells (CD20+ CD27+ CD43+) marginal zone B cells transitional B cells or antigen-experienced mature CD5+ B cells as have been proposed.30 43 Just as apoptotic cell infusions into mice stimulate natural Ab producing B cells 46 MEACs Isradipine may stimulate CLL cells in a similar manner. (ecologicalsgardens.com)
2003. Caspase-8 and apoptosis-inducing factor mediate a cytochrome c-independent pathway of apoptosis in human colon cancer cells induced by the dietary phytochemical chlorophyllin. . (oregonstate.edu)
Ras signaling is constitutively active in a large number of human cancers. (oncotarget.com)

The enhanced motility of vin −/− and vinY822F cells is also shown to be due to a similar mechanism. (rupress.org)

DHX15 was highly expressed in hematopoietic stem cells and leukemia cells but was lowly expressed in mature blood cells. (oncotarget.com)
CD33 is absent on lymphocytes, platelets, erythrocytes, hematopoietic stem cells and non-hematopoietic cystem. (kentlandsinitiative.org)
Second-line chemotherapy regimens vary, depending on whether hematopoietic stem cell transplantation (HSCT) is being considered. (medscape.com)
Chronic myeloid leukemia (CML) is a clonal expansion of the progenitor hematopoietic stem cells arising from the existence of the fusion BCR-ABL oncogene, that represents 15%-20% of the newly diagnosed cases of leukemia patients [ 1 - 2 ]. (jcancer.org)

Siglecs (sialic acid-binding immunoglobulin-like lectins) are single-pass cell surface receptors that have inhibitory activities on immune cells. (mdpi.com)

They include antibodies directed exclusively against the active form of the caspase. (bdbiosciences.com)

Significantly, birinapant coupled with third-generation MDR1i efficiently wiped out murine leukemic stem cells (LSCs) and long term success of AML-burdened mice, recommending a therapeutic chance for AML. (kentlandsinitiative.org)
It is also a key mediator of immune inflammatory hypernociception normally associated with a Th1 type of response, revealing a hitherto unrecognized function of IL-33 in a key immune pharmacological pathway that may be amenable to therapeutic intervention [ 13 ]. (hindawi.com)

According to recent data, Ras-transformed cells require autophagy to survive and maintain malignant phenotype [ 5 - 10 ]. (aging-us.com)
Given that autophagy is initiated upon suppressed mTORC1 activity, a protective autophagy in Ras-transformed cells with a high level of mTORC1 activity appears to be mTORC1-independent [ 20 , 21 ]. (aging-us.com)
Macroautophagy (thereafter designated as autophagy) is a self-digestive system conserved in all eukaryotic cells. (spandidos-publications.com)
The process of autophagy in mammalian cells involves six principal actions: initiation, nucleation, elongation, closure, maturation, buy 480-11-5 and degradation [27]. (immune-source.com)
Ras can also regulate autophagy by affecting several signaling pathways including the mTOR pathway. (oncotarget.com)
Autophagy and necroptosis were also detected in colo320 cell. (biomedcentral.com)

Thus, we conclude that ADF/cofilin activity is not generally required for induction or progression of apoptosis in mammalian cells. (nature.com)
In summary, our work demonstrates that in mammalian cells ADF/cofilin activity is not generally required for efficient induction of apoptosis. (nature.com)
We will also discuss potential approaches to humanize yeast in order to create modification patterns resembling the situation in mammalian cells, which could enhance the value of Saccharomyces cerevisiae and Kluyveromyces lactis as disease models. (microbialcell.com)

The diagnosis of diffuse large cell lymphoma is usually confirmed after positive findings are obtained from a lymph node biopsy specimen. (medscape.com)

Anatomy30

Organisms12

Diseases3

Chemicals and Drugs113

Analytical, Diagnostic and Therapeutic Techniques and Equipment27

Phenomena and Processes72

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Health Care1

Proteins17

Protein31

Proliferation12

Activated by multiple pathways1

Receptor12

Inhibitors9

Mechanisms9

Cleavage4

Viability11

Tissues7

Tumor cells6

Mitochondrial pathway1

Assay4

Differentiation10

Downstream3

Antibody12

Flow Cytometry4

Molecular3

Molecules2

Activates2

Transduction pathways1

Intracellular3

Activation22

Cytokine4

Genes9

Mitochondria4

Reagents2

Gene expression2

Posttranslational modifications1

FASL8

MRNA1

Tumors3

Inhibition8

Inhibit3

Apoptotic cell loss1

Extracellular2

Regulation3

Precursor1

Effector1

Cytotoxicity3

Intrinsic2

Expression4

Induces2

Proteases1

Assays3

Suppression2

Recombinant1

PI3K2

Mutation1

Cellular1

Proteolytic1

Death21

Membrane3

Chromatin condensation1

Human10

Motility1

Hematopoietic4

Receptors1

Antibodies1

Therapeutic2

Autophagy6

Mammalian cells3

Findings1