Caspase inhibitory protein. Medical search. Frequent questions

Furthermore, TNF α and Fas induced the cleavage of mitogen-activated protein kinase/ERK kinase kinase (MEKK)1, resulting in generation of a constitutive active form of MEKK1 leading to JNK activation in c-FLIP knockdown cells. (nature.com)
Communication between the pathways exists through cleavage of Bcl-2 interacting domain (Bid) by active caspase-8 to form truncated Bid (tBid). (cdc.gov)
This also led to the induction of the cleavage of caspase‑8 and caspase‑9 and resulted in the sensitization of highly resistant established GBM and patient‑derived GBM stem cell (GSC) lines to TRAIL‑mediated apoptosis. (spandidos-publications.com)
In the present study, we reported that celastrol potentiated tumor necrosis factor-α (TNF-α)-induced apoptosis, affected activation of caspase-8, caspase-3 and PARP cleavage, and inhibited the expression of anti-apoptotic proteins such as cellular inhibitor of apoptosis protein 1 and 2 (cIAP1 and cIAP2), cellular FLICE-inhibitory protein (FLIP), and B-cell lymphoma 2 (Bcl-2). (spandidos-publications.com)
We isolated primary tumor cells from 13 astrocytoma and oligoastrocytoma patients of all four WHO grades of malignancy and compared the levels of TRAIL-induced apoptosis induction, long-term tumor cell survival, caspase, and caspase target cleavage. (aacrjournals.org)
The cleavage of eIF4GI, which specifically needs caspase-3 activity, is dispensable for the inhibition of translation in MCF-7 cells. (gla.ac.uk)
In turn, caspase 8 activates effector caspases by catalytic cleavage. (biomedcentral.com)
Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. (appliedbioinfo.com)
Active caspase-8 tetramers are generated by cis- and transcatalytic cleavage from pro-caspase-8 homodimers [ 8 ]. (biomedcentral.com)
These cleavage steps are inhibited by heterodimer formation of caspase-8 with c-FLIP. (biomedcentral.com)

Inhibitors of apoptosis (IAPs) can prevent caspase activation under certain conditions. (cdc.gov)
The results were confirmed using caspase specific inhibitors and caspase activity assays. (cdc.gov)
Cell death is frequently considered to be 'caspase-dependent' when it is suppressed by broad-spectrum caspase inhibitors such as N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (Z-VAD-fmk). (geneontology.org)
In the cytosol, Smac/DIABLO interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. (biomedcentral.com)
Given the role of caspases in apoptosis, caspase inhibitors may provide neuronal protection after cardiac arrest. (asahq.org)
however, the selective inhibitors merely experienced partial inhibitory effects on these biological responses. (cell-signaling-pathways.com)

This protein binds inhibitor of apoptosis proteins (IAPs), thus freeing caspases to activate apoptosis. (wikipedia.org)
Receptor targeted toxins upregulated TRAIL death receptors (DR4 and DR5) and suppressed the expression of anti‑apoptotic FLICE‑inhibitory protein (FLIP) and X‑linked inhibitor of apoptosis protein (XIAP). (spandidos-publications.com)
Second mitochondria-derived activator of caspase/direct inhibitor of apoptosis-binding protein with low pI (Smac/DIABLO) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. (biomedcentral.com)
This protein family is comprised by X-linked inhibitor of apoptosis (XIAP), cellular IAP-1 (c-IAP1), cellular IAP-2 (c-IAP2), Testis specific IAP (Ts-IAP), survivin, livin and BRUCE/Apollon [ 8 ]. (biomedcentral.com)
Smac (Second mitochondria-derived activator of caspase) protein, also known as DIABLO (Direct Inhibitor of Apoptosis-Binding protein with LOw pI), is codified by a nuclear gene. (biomedcentral.com)

Caspase-8 (FLICE/MACH-1) is a 55 kDa cytosolic protein with homology to the CD95/Fas-associated signal transducer, FADD/MORT-1, as well as to other caspase (ICE/Ced-3) cysteine proteases. (bdbiosciences.com)
The process of apoptosis is executed by a family of cysteine proteases called caspases. (duke.edu)
Apoptosis is a programmed form of cell death involving the degradation of cellular constituents by a group of cysteine proteases called caspases. (geneontology.org)

To this end, the authors generated a transgenic rat line expressing baculovirus p35, a broad-spectrum caspase inhibitor, in central neurons. (asahq.org)

While cytochrome c directly activates APAF1 and caspase 9, SMAC binds IAPs, such as XIAP and cIAP proteins, to inhibit their caspase-binding activity and allow for caspase activation of apoptosis. (wikipedia.org)
There, Smac/DIABLO has a pro-apoptotic effect that is mediated by its interaction with IAPs and the release of caspases from them. (biomedcentral.com)
Functional autophagy mitigates neuronal apoptosis, which is reflected by the alterations of IAPs, Bcl-2, caspase-3, and so forth. (biomedcentral.com)

Nuclear factor-kappa B (NF- κ B) inhibits cell death through suppression of the caspase cascade, the c-Jun N-terminal kinase (JNK) pathway, and reactive oxygen species (ROS) accumulation. (nature.com)
In contrast, treatment of cells with the DNA-damaging agent etoposide inhibits protein synthesis equally well in the presence of a pan-specific caspase inhibitor and in the presence or absence of PKR. (gla.ac.uk)
The linker region between the BIR1 and BIR2 is implicated in the inhibition of caspase-3 and -7 whereas the BIR2 domain inhibits caspase-7 in a non-competitive manner [ 9 ]. (biomedcentral.com)
Accordingly, the neuroprotective mechanism of Rg1 is related to the activity that inhibits myosin IIA-actin interaction and the caspase-3/ROCK1/MLC signaling pathway. (ijbs.com)

We have recently shown that tumor necrosis factor (TNF) α induces caspase-dependent and -independent JNK activation and ROS accumulation in cellular FLICE-inhibitory protein (c-Flip) −/− murine embryonic fibroblasts (MEFs). (nature.com)
Consistent with the results using c-Flip −/− MEFs, we found that TNF α stimulation induced caspase-dependent prolonged JNK activation and ROS accumulation, followed by apoptotic and necrotic cell death in various tumor cells. (nature.com)
SMAC is a mitochondrial protein that promotes cytochrome c- and TNF receptor-dependent activation of apoptosis by inhibiting the effect of IAP - a group of proteins that negatively regulate apoptosis, or programmed cell death. (wikipedia.org)
FADD therefore acts as an adapter molecule, allowing caspase-8 to become recruited to the cytoplasmic region of Fas following receptor activation. (bdbiosciences.com)
Viral proteins (v-FLIPS) which inhibit recruitment and activation of caspase-8 have been isolated. (bdbiosciences.com)
CONCLUSION: Ellagic acid exhibited both antioxidant activity in V79-4 cells and apoptosis-inducing activity in HOS cells through the up-regulation of Bax and activation of caspase-3. (nutrimedical.com)
This leads to the activation of caspases and apoptosis of the cell. (dadamo.com)
Apoptosis triggered by meriolins is accompanied by rapid Mcl-1 down-regulation, cytochrome c release, and activation of caspases. (rcsb.org)
as a potential target for cancer therapy, because of its importance in suppressing metabolic suppression of caspase-2 activation and apoptosis. (duke.edu)
Apoptotic signaling is initiated either through extrinsic or intrinsic stimulation, resulting in the activation of caspases [ 12 ]. (biomedcentral.com)
BCL2 proteins are described as 'crucial regulators of apoptosis' (PMID:15868100)and the intrinsic apoptosis pathway is initiated by the release of mitochondrial cytochrome c into the cytoplasm, which results in the activation of caspases (15102863) - help in generating a defintion for intrinsic apoptosis? (geneontology.org)
should the term ['GO:0006919] activation of caspase activity' be a child of positive regulation of apoptosis or induction of apoptosis rather than directly to apoptosis? (geneontology.org)
does all activation of caspases require other proteins also involved in apoptosis - therefore shouldn't this term be a part of apoptosis? (geneontology.org)
Cytochrome c then forms a multi-protein complex known as the 'apoptosome' and initiates activation of the caspase cascade through caspase 9. (geneontology.org)
Their function is regulated by the activation of a number of activating and inhibitory receptors that bind to specific ligands expressed on the surface of target cells. (frontiersin.org)
Apoptosis is initiated by binding to receptors DR4/DR5 (TRAIL-R1, 2), which is followed by assembly of the death inducing signaling complex and activation of caspase-8. (biomedcentral.com)
Subsequent activation of caspases-3/7 (in a mitochondria-dependent or independent fashion) leads to execution of apoptosis [ 4 ]. (biomedcentral.com)
These findings suggest that neuronal apoptosis after cardiac arrest is not primarily initiated by activation of caspases. (asahq.org)
Aspp2 induced apoptosis protein expression in Aspp2-ad and combination groups, the Aspp2, Bax and activation of caspase-3 expression increased greatly both in vitro and in vivo . (scintica.com)
These effects were mediated via the down-regulation of caspase-3, ROCK1 (Rho-associated kinase1) activation and myosin light chain (MLC, Ser-19) phosphorylation. (ijbs.com)
2 , 3 A key feature of apoptosis has been defined as the maintenance of an intact cellular membrane (detectable as exclusion of impermeable dyes such as propidium iodide, PI) throughout the apoptotic program to prevent intracellular protein release and subsequent immunological activation. (nature.com)
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1a induced cell death. (lu.se)
Furthermore, in influenza A virus-infected cells, TG6-44 treatment led to suppression of virus-induced cell death as evidenced by decreased caspase-3 activation, decreased proportion of Annexin V+PI+ cells, and increased Bcl-2 phosphorylation. (cdc.gov)

Phosphorylation at CDK1, CDK4, and CDK9 sites on, respectively, protein phosphatase 1alpha, retinoblastoma protein, and RNA polymerase II is inhibited in neuroblastoma SH-SY5Y cells exposed to meriolins. (rcsb.org)
This metabolic suppression of caspase-2 is exerted via the inhibitory phosphorylation of S135 on the caspase-2 prodomain by activated Ca2+/Calmodulin-dependent protein kinase II (CaMKII). (duke.edu)
This inhibition is associated with the increased phosphorylation of eukaryotic initiation factor (eIF2) α, increased association of eIF4E with the inhibitory eIF4E-binding protein (4E-BP1), and specific cleavages of eIF4B and eIF2α. (gla.ac.uk)
Antibody Microarray Analyses of Signal Transduction Protein Expression and Phosphorylation during Porcine Oocyte Maturation. (appliedbioinfo.com)
Increases in apparent protein levels of protein kinases accounted for most of the detected changes during the GV to MI transition, whereas reduced protein kinase levels and increased protein phosphorylation characterized the MI to MII transition. (appliedbioinfo.com)
The pathological characteristics of AD are exhibited by the extracellular plaques of amyloid beta (Aβ) peptides and the hyper-phosphorylation of tau protein in neurofibrillary tangles [ 6 ]. (biomedcentral.com)

Jeffrey, I. W. , Bushell, M. , Tilleray, V. J. , Morley, S. and Clemens, M. J. (2002) Inhibition of protein synthesis in apoptosis: differential requirements by the tumor necrosis factor alpha family and a DNA-damaging agent for caspases and the double-stranded RNA-dependent protein kinase. (gla.ac.uk)
Similar experiments with embryonic fibroblasts from control mice and animals defective for expression of the double-stranded RNA-regulated protein kinase (PKR) reveal requirements for both caspase activity and PKR for inhibition of protein synthesis in response to TNFα. (gla.ac.uk)
This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. (appliedbioinfo.com)
This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. (cancerindex.org)
This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. (cancerindex.org)

Bax expression was induced and caspase-3 was activated by ellagic acid treatment. (nutrimedical.com)
Inhibitory peptides and fragments of TSP1 bind to CD36, leading to the expression of FAS ligand (FasL), which activates the expression of Fas. (dadamo.com)
Western blot analysis and real-time PCR showed that celastrol dose-dependently suppressed TNF-α-induced MMP-9 gene expression at both the mRNA and protein levels in MDA-MB-231 cells. (spandidos-publications.com)
The expression of caspase-3 and caspase-9 genes increased in HCT-116 cell lines after 48 h of treatment, suggesting cell death by the apoptotic pathways. (mendeley.com)
Bufalin was found to induce the apoptosis of GSCs by upregulating the expression of the apoptotic proteins cleaved caspase 3 and poly(ADP-ribose) polymerase (PARP) and by downregulating the expression of human telomerase reverse transcriptase, which is a marker of telomerase activity. (techscience.com)
C1QBP promoted the mRNA and protein expression of hypoxanthine catabolic enzyme XDH. (medsci.org)
C1QBP significantly increased ROS level, apoptosis, and the expression of apoptotic proteins such as cleaved caspase-3 and bax/bcl2 via regulating XDH. (medsci.org)
Caspase-3 expression increased significantly in all media containing F and R fractions, and also in the presence of BCH or WPI. (greenmedinfo.com)
MicroRNAs (miRNAs) are a kind of small noncoding RNAs that regulate the expression of protein through targeting the 3′UTR of messenger RNA (mRNA), resulting in mRNA degradation or suppression of translation [ 5 ]. (degruyter.com)
It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. (cancerindex.org)
Scope includes mutations and abnormal protein expression. (cancerindex.org)
Additionally, BRDs contain several catalytic domains that enable them to act as methyltransferases, ATP-dependent re-modellers or histone acetyltransferases and helicases ( 8 ) Bromodomain and extra-terminal domain (BET) proteins are a family of transcriptional mediators that regulate gene expression ( 8 , 9 ). (frontiersin.org)
Protein expression of ER stress and apoptosis factors was analyzed using Western Blot analysis. (karger.com)
Res effectively suppress the cardiomyocytes hypertrophy and apoptosis induced by ISO, characterized by the reduction of the myocardial cell surface area, the ANP gene expression, the LDH and MDA leakage amount and the rate of cell apoptosis, while decrease of the protein expression of GRP78, GRP94 and CHOP, and reverse the expression of Bcl-2 and Bax. (karger.com)
The results of western blotting showed the expression levels of caspase-9 and caspase-3 proteins increased following aloe-emodin treatment. (biomedcentral.com)
Our results revealed that aloe-emodin treatment could inhibit cell viability of SCC15 cells and the potential mechanism of inhibition might be through the induction of apoptosis by regulation of the expression levels of caspase-9 and caspase-3. (biomedcentral.com)
Our results showed that aloe-emodin could induce SCC15 cells apoptosis, moreover, the expression levels of caspase-9 and caspase-3 increased suggesting that the potential mechanism of aloe-emodin induces apoptosis might by regulating the caspases in SCC15 cells. (biomedcentral.com)
In conclusion, Aspp2-ad, in P53-independent manner, regulated ERK and STAT3 signal moleculars to inhibit hepatocarcinoma in coordination with oxaliplatin by influencing the protein expression of proliferation, apoptosis, autophagy and vascular growth. (scintica.com)

PMID:14996496, table 1 provides links to papers that describe subcellular location of many pro- and anti-apoptotic proteins. (geneontology.org)
In vitro , TRAIL-mediated apoptosis of prostate cancer cell lines can be enhanced by doxorubicin and correlates with the downregulation of the anti-apoptotic protein c-FLIP. (biomedcentral.com)
d This anti-apoptotic effect could be reversed by co-incubation with the CXCR7-selective antagonist CCX733 (0.1?M) but not significantly by CXCR4-selective antagonist AMD3100 (5?M). Both antagonists experienced no inhibitory effects on their own (not shown). (cell-signaling-pathways.com)

MCF-7) and this phenotype is blocked by overexpression of the caspase-3 protease inhibitor, CrmA. (bdbiosciences.com)
In the intrinsic pathway, several adverse factors act upon mitochondria to cause loss of the mitochondrial membrane potential, resulting in leakage into the cytosol of cytochrome C (Cyto C), which together with apoptotic protease activating factor 1 forms the apoptosome that activates caspase-9. (cdc.gov)
Conclusion: Human AAT is an acute phase protein with a broad-protease inhibitory and immunomodulatory activities used as a therapeutic for emphysema patients with inherited AAT deficiency. (lu.se)

The inhibitor of death receptor signaling, FLICE-inhibitory protein defines a new class of tumor progression factors. (nature.com)
Carbon nanotube s induce apoptosis resistance through FLICE-inhibitory protein. (cdc.gov)
DNA microarray and Western blot analyses of key apoptosis-regulatory proteins in the transformed cells revealed FLICE-inhibitory protein (FLIP) as an important target of regulation by SWCNT. (cdc.gov)

This in turn induces the recruitment of adapter proteins (FADD, TRADD o RAIDD) to form the so-called death-inducing signal complex (DISC), which activates caspase-8. (biomedcentral.com)

BD Biosciences Pharmingen offers several caspase-8 antibodies. (bdbiosciences.com)
In separate work, my team identified a set of secreted proteins overexpressed in the tumor microenvironment, and developed antibodies to block their cognate receptors for therapeutic gain. (gene.com)

The molecular docking studies showed favorable binding affinity against different pro- and antiapoptotic proteins by these compounds. (mendeley.com)

Full-length recombinant human caspase-8 protein was used as immunogen. (bdbiosciences.com)

The viability of the HEK-293 normal cells was found to be significantly higher than the viability of the cancerous cells at all of the tested concentrations, thus suggesting that all the compounds have better inhibitory activity on the cancer cell line. (mendeley.com)
OBJECTIVE: Investigation of the inhibitory capacity on melanoma cells (B16F10) proliferation and the influence on % distribution of cell cycle phases, in the presence of various concentrations of whey protein isolate (WPI), bovine collagen hydrolysate (BCH) or its fractions. (greenmedinfo.com)
RESULTS: The inhibitory concentrations for 50% of B16F10 cells (IC(50)) ranged from 0.19 to 156.9 mg/mL for all these proteins evaluated. (greenmedinfo.com)
The most inhibitory fractions of the BCH hydrolysate were BCH-P1 and F1-F4 with IC(50) concentrations below 1mg/mL. (greenmedinfo.com)
Physcion, the half maximal inhibitory concentrations (IC 50 ) value of aloe-emodin was 60.90 μM at 48 h of treatment. (biomedcentral.com)
The key regulator of this process, hypoxia inducible factor 1 (HIF-1), can initiate apoptosis by inducing high concentrations of proapoptotic proteins, such as BNIP3, and can cause stabilisation of p53. (bmj.com)

microRNAs (miRNAs) are important regulators of target messenger RNA translation by binding mainly to complementary sequences of the 3′ untranslated region of target messenger RNA transcripts thereby leading to RNA degradation and/or inhibition of protein synthesis [ 4 ]. (hindawi.com)
Exposure of mammalian cells to agents that induce apoptosis results in a rapid and substantial inhibition of protein synthesis. (gla.ac.uk)

This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. (cancerindex.org)
The effector caspases cleave their cellular specific substrates and generate the typical morphology of apoptosis. (biomedcentral.com)
Dying cells have been defined as apoptotic by distinguishing features, including cell contraction, nuclear fragmentation, blebbing, apoptotic body formation and maintenance of intact cellular membranes to prevent massive protein release and consequent inflammation. (nature.com)

For apoptotic inducing activity, human osteogenic sarcoma (HOS) cell proliferation, chromosomal DNA degradation and changes in apoptosis-related protein levels were measured. (nutrimedical.com)

The apoptosis inhibitor c-FLIP is structurally similar to caspase-8 but lacks the cysteine residue essential for catalytic activity. (biomedcentral.com)

Overexpression of caspase-8 is sufficient to induce apoptosis in certain cell lines (e.g. (bdbiosciences.com)
Considering the ability of HP1286 to induce macrophage apoptosis, the protein could probably help in the bacterial escape from the triggered macrophages and perseverance in the belly. (thetechnoant.info)
TRAIL is a type II membrane protein that can induce apoptosis by binding to death domain containing receptors DR4 and DR5 [ 4 ]. (biomedcentral.com)

CONCLUSION: It is suggested that a mechanism for tumorigenesis inhibition may involve the caspases cascade and apoptosis. (greenmedinfo.com)

Moreover, TGFBR2 was a direct target of miR-128-3p, and its overexpression reversed the inhibitory effects of miR-128-3p overexpression on inflammation and apoptosis in LPS-induced HK2 cells. (degruyter.com)

Through the intrinsic pathway of apoptosis, BCL-2 proteins like BAK and BAX form a pore in the outer mitochondrial membrane, leading to mitochondrial membrane permeabilization and the release of both cytochrome c and SMAC. (wikipedia.org)
Caspase-2, an apoptotic initiator, can be suppressed by high levels of nutrient flux through the pentose phosphate pathway (PPP). (duke.edu)
Bufalin also improved the inhibitory effect of TMZ on GSCs by activating the mitochondrial apoptotic pathway. (techscience.com)

In the presence of divalent cations, the secreted protein binds to the surface of the activated platelets and is responsible for the endogenous lectin-like activity associated with activated platelets. (dadamo.com)
Meriolins thus constitute a new CDK inhibitory scaffold, with promising antitumor activity, derived from molecules initially isolated from marine organisms. (rcsb.org)
The caspase-3 and caspase-9 expressions and DAPI (4′, 6-diamidino-2-phenylindole) staining assays were used to evaluate the apoptotic activity. (mendeley.com)
In MCF-7 breast cancer cells, tumor necrosis factor α (TNFα) and TNF-related apoptosis-inducing ligand inhibit overall translation by a mechanism that requires caspase (but not necessarily caspase-3) activity. (gla.ac.uk)
All of these changes require caspase activity. (gla.ac.uk)
Lacks enzymatic (caspase) activity. (ningbobio.com)
Although the presence of active caspases and DNA fragmentation is helpful in identifying possible apoptosis, they should not be employed as an exclusive means to demonstrate this process as apototic cell death can occur without DNA fragmentation or caspase activity. (geneontology.org)
Blebbing, apoptotic body formation and protein release during early apoptosis are dependent on ROCK and myosin ATPase activity to drive actomyosin contraction. (nature.com)

The homodimer structure also facilitates SMAC-XIAP binding via the BIR2 domain, though it does not form until the protein is released into the cytoplasm as a result of outer mitochondrial membrane permeabilization. (wikipedia.org)

To demonstrate the inhibitory effect of bufalin on glioma cells and glioma stem-like cells (GSCs) and discuss the underlying mechanism, the proliferation of glioma cells was detected by MTT and colony formation assays following treatment with bufalin. (techscience.com)

N-acetyl amino acids can be produced either via direct synthesis of specific N-acetyltransferases or via the proteolytic degradation of N-acetylated proteins by specific hydrolases. (hmdb.ca)

Likely target for the cowpox virus CRMA death inhibitory protein. (lu.se)

These results indicate that limited membrane permeabilization occurs in blebs and apoptotic bodies before secondary necrosis, leading to acute and localized release of immunomodulatory proteins during the early phase of active apoptotic membrane blebbing. (nature.com)

We now show that during early apoptosis limited membrane permeabilization occurs in blebs and apoptotic bodies, which allows release of proteins that may affect the proximal microenvironment before the catastrophic loss of membrane integrity during secondary necrosis. (nature.com)

Thrombospondins (TSP) are secreted proteins with the ability to inhibit angiogenesis. (dadamo.com)

FLIP, Fas-associated death domain-like interleukin-1β converting enzyme-like inhibitory protein. (cdc.gov)

Kinex antibody microarray analyses was used to investigate the regulation of 188 protein kinases, 24 protein phosphatases, and 170 other regulatory proteins during meiotic maturation of immature germinal vesicle (GV+) pig oocytes to maturing oocytes that had completed meiosis I (MI), and fully mature oocytes arrested at metaphase of meiosis II (MII). (appliedbioinfo.com)

SMAC is normally a mitochondrial protein localized to the mitochondrial intermembrane space, but it enters the cytosol when cells undergo apoptosis. (wikipedia.org)

The N-terminal region of caspase-8 contains an amino acid sequence, termed the death domain, that facilitates caspase-8-FADD direct interaction. (bdbiosciences.com)

Thus, monomeric SMAC mutants can still bind the BIR3 domain but not the BIR2 domain, which compromises the protein's inhibitory function. (wikipedia.org)

I joined the department of Molecular Biology as Scientist in 1989, and my lab helped establish a technology to fuse biologically important proteins to antibody molecules. (gene.com)

A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. (ningbobio.com)
BRDs can act as scaffolds that enable the recruitment of large protein complexes or they can act as transcription factors themselves. (frontiersin.org)

Given that ROS accumulation and necrotic cell death enhance inflammation followed by compensatory proliferation of tumor cells, selective suppression of caspase-dependent ROS accumulation will be an alternative strategy to protect cells from ROS-dependent DNA damage and compensatory tumor progression. (nature.com)
In vitro impact of a whey protein isolate (WPI) and collagen hydrolysates (CHs) on B16F10 melanoma cells proliferation. (greenmedinfo.com)

Protein kinases represent promising anticancer drug targets. (rcsb.org)
The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. (cancerindex.org)

Incomplete conversion of the sulfhydryl -SH group onto S-S disulfide bonds in the protein of the cortical fibers leads to cortical softness and subsequent invagination of the fully keratinized distal hair shaft into the softer, abnormally keratinized proximal hair shaft. (medscape.com)

Diablo homolog (DIABLO) is a mitochondrial protein that in humans is encoded by the DIABLO (direct IAP binding protein with low pI) gene on chromosome 12. (wikipedia.org)

Bromodomains are proteins that contain modules of ~110 amino acids that recognize and bind acetylated lysine residues in histones and other proteins. (frontiersin.org)
The most enriched proteins released were the nucleosomal histones, which have previously been identified as damage-associated molecular pattern proteins (DAMPs) that can initiate sterile inflammatory responses. (nature.com)

DIABLO is also referred to as second mitochondria-derived activator of caspases or SMAC. (wikipedia.org)

These mimetics are also designed to target tumor cells directly through interacting with inflammatory proteins, such as IL-1β, which are commonly produced by solid tumor lesions. (wikipedia.org)
Lysates from control (lanes 1-3) and camptothecin treated Jurkat cells (lanes 4-6) were probed with anti-human caspase-8 (clone 4-1-20, Cat. (bdbiosciences.com)
Caspase-8 is identified as 55/50 kDa (proform) and 40/36 kDa (cleaved) bands in treated cells and the 55 kDa in control cells. (bdbiosciences.com)
The UPR normally helps secretory cells to resolve issues with 3D shaping of newly synthesized proteins. (gene.com)
Previous studies revealed that bufalin exerted inhibitory effects on a variety of tumor cells. (techscience.com)
Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. (ningbobio.com)
Combination group showed a synergetic effect, the inhibitory rates were all above 80% at 48 h point in HepG2P53-/- and Hep3B cells. (scintica.com)
But interestingly, the autophagy proteins showed different responses not only in HepG2P53-/- and Hep3B cells but also in vitro and in vivo . (scintica.com)
[ 15 ] The surface of the APC contains 2 peptide-binding proteins (ie, major histocompatability complex [MHC] classes I and II), which can stimulate cytotoxic T (T C ) cells, regulatory T (Treg) cells, and helper T (T H ) cells. (medscape.com)

The full-length protein product spans 239 residues, 55 of which comprise the mitochondrial-targeting sequence (MTS) at its N-terminal. (wikipedia.org)
The substrate specificities of different NAT enzymes are mainly determined by the identities of the first two N-terminal residues of the target protein. (hmdb.ca)

Anatomy23

Organisms11

Diseases7

Chemicals and Drugs146

Analytical, Diagnostic and Therapeutic Techniques and Equipment16

Phenomena and Processes50

Disciplines and Occupations1

Information Science3

Cleavage10

Inhibitors6

Inhibitor of apopto5

Cysteine proteases3

Broad-spectrum caspase1

IAPs3

Inhibits4

Activation23

Phosphorylation6

Kinase5

Expression18

Anti-apoptotic3

Protease3

FLICE3

Induces1

Antibodies2

Antiapoptotic1

Recombinant human1

Concentrations6

Inhibition of protein synthesis2

Cellular3

Apoptosis-related1

Catalytic1

Induce3

Cascade1

Overexpression1

Pathway3

Activity8

XIAP1

Assays1

Degradation1

CrmA1

Immunomodulatory1

Release of proteins1

Angiogenesis1

FLIP1

Phosphatases1

Intermembrane space1

FADD1

BIR21

Molecules1

Recruitment2

Proliferation2

Kinases2

Subsequent1

Mitochondrial protein1

Histones2

SMAC1

Cells9

Residues2