Caspase 1 and caspase. Medical search. Frequent questions

Owing to the importance of CARD-CARD interactions in inflammasome formation, many COPs are known inhibitors of Caspase activation. (wikipedia.org)
Inhibitors Belnacasan (VX-765) Pralnacasan (VX-740) Activated Caspase 1 proteolytically cleaves pro IL-1β and pro IL-18 into their active forms, IL-1β and IL-18. (wikipedia.org)
Useful in studying enzyme regulation and inhibitors, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. (enzolifesciences.com)
Active recombinant caspases are useful in screening caspase inhibitors, studying enzyme kinetics and regulation, determining substrate specificity, and as a positive control in caspase activity assays. (enzolifesciences.com)
2018) Rational Drug Design of Topically Administered Caspase 1 Inhibitors for the Treatment of Inflammatory Acne. (guidetoimmunopharmacology.org)
2005) Novel pyrazinone mono-amides as potent and reversible caspase-3 inhibitors. (guidetoimmunopharmacology.org)
2005) Caspase inhibitors: a pharmaceutical industry perspective. (guidetoimmunopharmacology.org)
The three main classes of caspase inhibitors frequently found among viruses include serpins (CrmA/SPI-2), viral IAPs (vIAPs) and p35. (edu.pl)
2001) Potent and selective nonpeptide inhibitors of caspases 3 and 7. (guidetopharmacology.org)

Description: A polyclonal antibody for detection of Caspase-1 from Human, Mouse, Rat. (bd-ibr.org)
This Caspase-1 antibody is for WB, IHC-P, ELISA. (bd-ibr.org)
Human IgG antibody Laboratories manufactures the signalway antibody caspase-1 reagents distributed by Genprice. (bionotatki.com)
The Signalway Antibody Caspase-1 reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (bionotatki.com)
Specificity : The mouse monoclonal antibody 15-414 recognizes en extracellular epitope of CD172a (SIRP alpha), an approximately 90 kDa transmembrane glycoprotein expressed on cells of myeloid origin and neurons. (abeomics.com)
Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 19677-1-AP (Caspase 3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. (ptgcn.com)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 10x lens. (ptgcn.com)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 40x lens. (ptgcn.com)
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Caspase 3/p17/p19 (19677-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. (ptgcn.com)

Once activated through formation of an inflammasome complex, it initiates a proinflammatory response through the cleavage and thus activation of the two inflammatory cytokines, interleukin 1β (IL-1β) and interleukin 18 (IL-18) as well as pyroptosis, a programmed lytic cell death pathway, through cleavage of Gasdermin D. The two inflammatory cytokines activated by Caspase-1 are excreted from the cell to further induce the inflammatory response in neighboring cells. (wikipedia.org)
Cleavage of pNA peptides by caspases generates pNA that is monitored colorimetrically at ~405 nm. (eurogentec.com)
Injury-induced cytochrome c -specific cleavage of caspase-9 followed by activation of caspase-3 in mature brain correlated with marked increases in Apaf-1 and caspase-3 mRNA and protein expression. (jneurosci.org)
CAD release from ICAD inhibition is achieved by cleavage of ICAD at these Asp residues by the caspase-3. (wikipedia.org)
Furthermore, TNF α and Fas induced the cleavage of mitogen-activated protein kinase/ERK kinase kinase (MEKK)1, resulting in generation of a constitutive active form of MEKK1 leading to JNK activation in c-FLIP knockdown cells. (nature.com)
Caspase-1 cleavage of the TLR adaptor TRIF inhibits autophagy and β-interferon production during Pseudomonas aeruginosa infection. (ox.ac.uk)
Preventing TRIF cleavage by caspase-1 in an in vivo model of P. aeruginosa infection resulted in enhanced bacterial autophagy, attenuated IL-1β production, and increased bacterial clearance. (ox.ac.uk)
Additionally, TRIF cleavage by caspase-1 diminished NLRP3 inflammasome activation. (ox.ac.uk)
Thus, caspase-1 mediated TRIF cleavage is a key event in controlling autophagy, type I interferon production, and inflammasome activation with important functional consequences. (ox.ac.uk)
The canonical sequence involves pathogen-associated molecular patterns (PAMPs)- and damage-associated molecular patterns (DAMPs)-mediated inflammasome formation leading to caspase-1 activation, GSDMD cleavage, and IL-1 β and IL-18 maturation. (genetex.com)

The WEHD sequence is thought to be a "better" caspase-1 target, as the kcat/kM rate is higher for WEHD vs YVAD (meaning faster conversion of substrate product by the enzyme). (immunochemistry.com)
Routinely tested for its ability to cleave the caspase-1 colorimetric substrate Ac-YVAD- p NA (Prod. (enzolifesciences.com)
Ac-LEHD-AFC is a fluorogenic substrate for caspase-4, caspase-5, and caspase-9. (adooq.com)
DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. (wikipedia.org)
CD172a, the signal-regulatory protein alpha (SIRP alpha), also known as SH2 domain-containing phosphatase substrate-1 (SHPS1), is a 75-110 kDa transmembrane glycoprotein expressed mainly on granulocytes, monocytes, macrophages, dendritic cells and neurons. (abeomics.com)
Crystal structures of a ligand-free and malonate-bound human caspase-1: implications for the mechanism of substrate binding. (expasy.org)

We have recently shown that tumor necrosis factor (TNF) α induces caspase-dependent and -independent JNK activation and ROS accumulation in cellular FLICE-inhibitory protein (c-Flip) −/− murine embryonic fibroblasts (MEFs). (nature.com)
Recently, it was demonstrated that activation of PARP-1 induces translocation of apoptosis-inducing factor from the mitochondria to the nucleus, causing DNA condensation and fragmentation, and subsequent cell death . (bvsalud.org)

This in vitro assay employs the fluorescent inhibitor probe FAM-WEHD-FMK to label active caspase-1 enzyme in living cells. (immunochemistry.com)
We'll examine how ImmunoChemistry Technologies' FLICA (Fluorescent-Labeled Inhibitor of Caspases) assays can be used to assess cell death, including caspase-1 activation and pyroptosis. (immunochemistry.com)
combined BI2536 (an inhibitor of PLK1) with cisplatin (DDP) to treat ESCC cells and found the combination of two drugs had a synergistic effect on tumor inhibition and resulted in pyroptotic death of cancer cells by Caspase-3/GSDME pathway. (researchsquare.com)
Z-YVAD-FMK is a potent cell-permeable and irreversible inhibitor of caspase-1. (adooq.com)
Z-VDVAD-FMK is a cell-permeable, irreversible inhibitor of caspase-2. (adooq.com)
VX-765 is a novel Caspase-1 inhibitor,which is an enzyme that controls the generation of two cytokines, IL-1b and IL-18. (adooq.com)
Z-DEVD-FMK is a specific, irreversible Caspase-3 inhibitor. (adooq.com)
2007) (S)-1-((S)-2-{[1-(4-amino-3-chloro-phenyl)-methanoyl]-amino}-3,3-dimethyl-butanoyl)-pyrrolidine-2-carboxylic acid ((2R,3S)-2-ethoxy-5-oxo-tetrahydro-furan-3-yl)-amide (VX-765), an orally available selective interleukin (IL)-converting enzyme/caspase-1 inhibitor, exhibits potent anti-inflammatory activities by inhibiting the release of IL-1beta and IL-18. (guidetoimmunopharmacology.org)
Per usual in non-apoptotic growing cells caspase activated dnase is held in check inactivated in the cytoplasm thanks to the association with its inhibitor, inhibitor of caspase-activated DNase (ICAD) also known as DNA fragmentation factor 45 kDa (DFF45). (wikipedia.org)

Caspase-1 is the prototypical member of the caspase family of cysteine proteases. (enzolifesciences.com)
Description: Caspases, or cysteine-aspartic proteases, are a family of cysteine proteases that play essential roles in inflammation, apoptosis and necrosis. (bionotatki.com)
Description: Caspase-1 is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, interleukin 18, and interleukin 33 precursor proteins. (bionotatki.com)
It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. (bionotatki.com)

Activation of caspase-4 results in pyroptosis, and induction of NLRP3 inflammasome activation which promotes IL-1β and IL-18 maturation and secretion. (guidetopharmacology.org)
These results provide a unifying model for the activation of the NLRP3 inflammasome in which a drop in cytosolic K⁺ is the common step that is necessary and sufficient for caspase-1 activation. (nih.gov)
após a liberação de IL-18 pela via da caspase-1, é o principal mecanismo responsável pelo controle fúngico quando o dano celular desencadeado pelo fungo é reconhecido pelo inflamassoma de NLRP3. (usp.br)
mediated protective immune response following by caspase-1- dependent IL-18 release after is the key mechanism responsible for the fungal control after P. brasiliensis-induced cell damage recognition by NLRP3 canonical inflammasome pathway. (usp.br)
We found that C. burnetii upregulates pro-IL-1' and robustly primes NLRP3 inflammasomes via TLR2 and MyD88 signaling. (usda.gov)
Mice deficient in NLRP3 or caspase-1/11 were similar to wild-type mice in the development and resolution of splenomegaly, histologic lesions and macrophage kinetics, but a suggestion of slightly higher pulmonary bacterial burdens at peak infection. (usda.gov)

Here we demonstrate that, during brain maturation, the potential of the intrinsic apoptotic pathway is progressively reduced and that such repression is associated with downregulation of apoptotic protease-activating factor-1 (Apaf-1) and caspase-3 gene expression. (jneurosci.org)
These results suggest that differential expression of Apaf-1 and caspase-3 genes may underlie regulation of apoptotic susceptibility during brain development, as well as after acute injury to mature brain, through the intrinsic pathway of caspase activation. (jneurosci.org)
Eldadah and Faden, 2000 ), we hypothesized that suppression of apoptotic capability during maturation of mammalian brain results from repression of genes involved in the caspase-3 activation pathway, and that injury-induced neuronal apoptosis in the mature brain results from reactivation of these genes. (jneurosci.org)
Though inducing more significantly pyroptosis by activating Caspase-1/GSDMD pathway, OP-B relieved DDP resistance of A549 cells. (researchsquare.com)
The activation of classical and non-classical pathway is separately dependent on Caspase-1 or Caspase-4/5/11 (6, 7). (researchsquare.com)
Nuclear factor-kappa B (NF- κ B) inhibits cell death through suppression of the caspase cascade, the c-Jun N-terminal kinase (JNK) pathway, and reactive oxygen species (ROS) accumulation. (nature.com)
This entry was posted in Caspase Pathway by casp4137 . (caspasepathway.com)
In this review , an alternative intrinsic cell death pathway, mediated by poly(ADP-ribose) polymerase -1 (PARP-1) and apoptosis-inducing factor (AIF), is described. (bvsalud.org)
This pathway seems to be triggered by depletion of NAD (+) and appears to be caspase independent. (bvsalud.org)
Caspase-1 was significantly elevated in the liver at 6 h, to a greater extent in BALB/c mice, suggesting inflammasome pathway activation. (cdc.gov)
A major inflammatory pathway involved in IL-1? (usp.br)

Similarly, some POPs (Pyrin only proteins) are also known to regulate Caspase-1 activation through inhibition of inflammasome activation by binding to and blocking PYD interactions, which also play a role in the formation of the inflammasomes, though the exact mechanisms are not yet well established. (wikipedia.org)
Caspase-1 directly cleaved TRIF to diminish TRIF-mediated signaling, resulting in inhibition of autophagy and in reduced type I interferon production. (ox.ac.uk)
C. burnetii employs a Type IV secretion system (T4SS) to establish and maintain its intracellular niche and to modulate host immune responses including the inhibition of apoptosis, a form of caspase-mediated cell death. (usda.gov)

In this webinar, learn about the mechanisms of pyroptosis, including the critical role of caspase-1, a protease involved in activation of pro-inflammatory cytokines, plasma membrane rupture, and other morphological characteristics of pyroptosis. (immunochemistry.com)
The cone-enriched apoptotic protease caspase-7 ( Casp7 ) is thought to be triggered by endoplasmic reticulum (ER) stress and plays a pivotal role in mice deficient in the cone cyclic nucleotide-gated channels, a deficiency that causes achromatopsia in humans and in mice with autosomal dominant rhodopsin mutations, in particular the T17M mutation. (molvis.org)

Caspase-1/Interleukin-1 converting enzyme (ICE) is an evolutionarily conserved enzyme that proteolytically cleaves other proteins, such as the precursors of the inflammatory cytokines interleukin 1β and interleukin 18 as well as the pyroptosis inducer Gasdermin D, into active mature peptides. (wikipedia.org)
Cell morphological feature, mitochondrial membrane potential, LDH release rate, production of IL-1β and expression of Caspase-1/GSDMD all showed that pyroptosis happened more significantly in A549/DDP cells than that in A549 cells after OP-B treatment. (researchsquare.com)
Pyroptosis is a type of programmed cell death that associated with inflammation, which characterized by cell swelling, destruction of membrane structural integrity and cytoplasmic contents release, like interleukin-1β (IL-1β), interleukin-18 (IL-18) and lactate dehydrogenase (LDH) (5). (researchsquare.com)
Activated Caspase-1/4/5/11 catalyze GSDMD to cleave into GSDMD-N and GSDMD-C fragments, and GSDMD-N can bind and form pores at cell membrane, so as to lead pyroptosis and release IL-1β and IL-18 to extracellular environment (8, 9). (researchsquare.com)
Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages. (bionotatki.com)
Pyroptosis is a programmed cell death process executed by inﬂammatory caspases upon initiation of canonical or non-canonical mechanisms. (genetex.com)
C. burnetii is unable to inhibit pyroptosis and IL-1' secretion induced by potent inflammasome stimuli but rather enhanced these caspase-1-mediated effects. (usda.gov)

Previous studies with recombinant protein identified a caspase-1 tetramer composed of two p20 and two p10 subunits (p20/p10) as an active species. (altmetric.com)

The inflammasome complex is a ring complex composed of trimers of a signal specific sensor protein such as those of the NLR family and the AIM-1 (Absent in Melanoma) like receptors, an adaptor protein such as ASC, and a caspase, in this case Caspase-1. (wikipedia.org)
Caspase-activated DNase (CAD) or DNA fragmentation factor subunit beta is a protein that in humans is encoded by the DFFB gene. (wikipedia.org)
Caspase 3 is responsible for cellular differentiation, although it is unclear how this kind of protein can promote the cell apoptosis. (wikipedia.org)
After 24 h, the cells were harvested for Western blot analyses to detect the level of IFI16, caspase-1, pro-caspase-1, GSDMD-N, GSDMD-F, IL-1β, viral N protein and GAPDH. (biomedcentral.com)
and protein carbonyl, IL-1B, and caspase-1 determination. (cdc.gov)
AIM: To explore the effect of human plasma alpha-1 antitrypsin (AAT) protein in the chemically induced mouse model of colorectal cancer. (lu.se)
This complex in turn activates other protein complexes called nuclear factor-kappa-B (NF-κB) and mTOR complex 1 (mTORC1), which are important for cellular signaling. (medlineplus.gov)

Active caspase-9, in turn, activates executioner caspases-3 and -7. (jneurosci.org)
Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-κB. (nature.com)

A similar decline in apoptotic susceptibility associated with downregulation of Apaf-1 expression as a function of developmental age was also found in cultured primary rat cortical neurons. (jneurosci.org)
Caspase-3 is activated in the apoptotic cell. (wikipedia.org)
Consistent with the results using c-Flip −/− MEFs, we found that TNF α stimulation induced caspase-dependent prolonged JNK activation and ROS accumulation, followed by apoptotic and necrotic cell death in various tumor cells. (nature.com)
Caspases are key effectors of the apoptotic process. (edu.pl)

Caspase-1 mediates maturation of pro-forms of cytokines IL-1β and IL-18 into active forms. (ntnu.edu)
maturation, the lack of caspase-1 only partially reduced IL-1? (usp.br)

Amongst its substrates are the precursors of the inflammatory cytokines IL-1β and IL-18, which it proteolytically cleaves into active mature peptides. (guidetoimmunopharmacology.org)

NLRC4 and caspase-1 activation following infection attenuated autophagy. (ox.ac.uk)

Caspase-1 is produced as a zymogen that can then be cleaved into 20 kDa (p20) and 10 kDa (p10) subunits that become part of the active enzyme. (wikipedia.org)
Caspase-1, normally in its physiologically inactive zymogen form, autoactivates when it is assembled into the filamentous inflammasome complex by autoproteolysis into the p10 and p20 subunits. (wikipedia.org)
Active caspase-1 and -3 are heterodimers, containing two small and two large subunits. (bionotatki.com)
Active Caspase-3 subunits are 17kD (p17) and 12kD (p12). (bionotatki.com)

For example, ICEBERG nucleates the formation of Caspase-1 filaments and is thus incorporated into the filaments, but lacks the ability to inhibit the activation of inflammasomes. (wikipedia.org)
Instead, it is thought to inhibit Caspase-1 activation by interfering with the interaction of Caspase-1 with other important CARD containing proteins. (wikipedia.org)
Among pathogens, particularly viruses express various modulators of caspases that inhibit their activity by direct binding. (edu.pl)
TSN enhances the production of IL-1β to inhibit the replication of PRRSV. (biomedcentral.com)

This enhances the production of IL-1, tumor necrosis factor (TNF), caspase-1, and fibroblast growth factor (FGF). (medscape.com)

For Caspase-1, genes for specific COPs-ICEBERG, COP1 (ICE/Pseudo-ICE), and INCA (Inhibitory Card)-are all found near its locus, and are thus thought to have emerged from gene duplication events and subsequent deletions of the catalytic domains. (wikipedia.org)
The Caspase-1 gene consists of 10 exons spanning at least 10.6 kb and is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas. (bionotatki.com)
The Caspase-1 gene consists of 10 exons spanning at least 10.6 kb and is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal human metaphase chromosomes. (bionotatki.com)

It is activated by Caspases -8, -9 and -10 ('initiator caspases') and then effects apoptosis by cleaving targeted cellular proteins. (bionotatki.com)

Caspase 1 is also known as interleukin-1beta (IL-1α) converting enzyme (ICE). (guidetoimmunopharmacology.org)
Interleukin (IL)-1 is stimulated directly by macrophages and indirectly by toll-like receptors. (medscape.com)

Following infection, the inflammatory response increases expression of Caspase-1, by a positive feedback mechanism that amplifies the response. (wikipedia.org)
To date the mechanism of regulation of caspase-1 activation is complex and poorly understood. (enzolifesciences.com)
The inflammasome-caspase-1 complex thus functions as a holoenzyme that directs the location of caspase-1 activity but also incorporates an intrinsic self-limiting mechanism that ensures timely caspase-1 deactivation. (altmetric.com)
Thus, identifying a specific caspase as part of a cell death mechanism can give insights into the underlying cause for cell death. (molvis.org)

Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide (prodomain) together with one large and one small subunit. (bionotatki.com)

pNA (4-nitroaniline)-derived caspase substrates are widely used for the colorimetric detection of various caspase activities. (eurogentec.com)

Caspase-1 is activated on inflammasomes, signaling platforms that facilitate caspase-1 dimerization and autoprocessing. (altmetric.com)
In this study, we report that in the cell, the dominant species of active caspase-1 dimers elicited by inflammasomes are in fact full-length p46 and a transient species, p33/p10. (altmetric.com)
Inflammasomes are multi-molecular complexes that process pro-caspase-1 into the active enzyme. (ntnu.edu)
secretion is the activation of inflammasomes, large multimolecular complexes best known for their ability to control activation of the proteolytic enzyme caspase-1. (usp.br)
Interactions between C. burnetii and caspase-1-mediated inflammasomes are not fully elucidated. (usda.gov)

The caspase activation and recruitment domain (CARD) of caspase-4 recognises the lipid A moiety of LPS. (guidetopharmacology.org)

The remaining green fluorescent signal is a direct measure of the active caspase-1 enzyme activity present in the cell at the time the reagent was added. (immunochemistry.com)

ICT's FLICA assay kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues. (immunochemistry.com)
Add diluted FLICA to each sample at 1:30 (e.g., add 10 μL to 290 μL of cultured cells). (immunochemistry.com)
Caspase-1 exists in cells as an inactive 45kDa pro-enzyme. (enzolifesciences.com)
In THP-1 cells, a large proportion of the caspase-1 is present in the inactive pro-enzyme form. (enzolifesciences.com)
Given that ROS accumulation and necrotic cell death enhance inflammation followed by compensatory proliferation of tumor cells, selective suppression of caspase-dependent ROS accumulation will be an alternative strategy to protect cells from ROS-dependent DNA damage and compensatory tumor progression. (nature.com)
Figure 1 : Flow cytometry analysis (surface staining) of human peripheral blood cells using anti-CD172a (15-414) purified, GAM-APC. (abeomics.com)
IEC expulsion was accompanied by a major actin rearrangement in neighboring cells that maintained epithelium integrity but did not absolutely require Caspase-1 or Gasdermin D. Analysis of Casp1 -/- Casp8 -/- mice revealed a functional Caspase-8 inflammasome in vivo. (elsevierpure.com)
incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3 transfected Jurkat cells. (ptgcn.com)
Detection:19677-1-AP 1:300) with NIH/3T3 cells lysate 3440 ug. (ptgcn.com)
These cells display the characteristic markers of AAM including upregulated arginase-1 (arg-1), Ym1, FIZZ and also expressed IL-10. (biomedcentral.com)
C - F ) Marc-145 cells grown in 24-well plates were infected with PRRSV NADC30-like (0.05 MOI) for 2 h at 37 ℃ and then cultured in fresh media containing various concentrations of IL-1β. (biomedcentral.com)

Active Caspase 1 contains two heterodimers of p20 and p10. (wikipedia.org)
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. (kamiyabio.com)
The caspase-3 His-237 stabilizes the target Aspartate causing the break of the association of ICAD and CAD leaving the endonuclease CAD active allowing it to degrade chromosomal DNA. (wikipedia.org)

The FLICA reagent FAM-WEHD-FMK enters each cell and irreversibly binds to activated caspase-1. (immunochemistry.com)

Expression of a caspase-1 resistant TRIF mutant enhanced autophagy and type I interferon production following infection. (ox.ac.uk)
Infection with the protozoan parasite T. gondii can occur via the oral route, the foetal-maternal interface, or by consumption of undercooked meat containing parasitic cysts [ 1 ]. (biomedcentral.com)
Completed and published a study on the roles of caspase-8 and RIP kinases inflammasome activation, NF-κB activation and cell death in response to bacterial infection. (ntnu.edu)
In this study we addressed the mechanisms that underlie canonical and non-canonical inflammasome pathways, assessing the functional importance of caspase-1, caspase-11 and caspase-8 in the regulation of inflammasome-mediated host resistance during P. brasiliensis infection. (usp.br)
We show that C. burnetii does not activate caspase-1 during infection of mouse macrophages in vitro. (usda.gov)

Thus, a coordinated IEC-intrinsic, Caspase-1 and -8 inflammasome response plays a key role in intestinal immune defense and pathology. (elsevierpure.com)

Inflammasome is a multiproteic complex that assists the activation of various inflammatory caspases in response to environmental signals or the entry of pathogens into a cell. (fapesp.br)
1. Avivi-Green C, Polak-Charcon S, Madar Z, Schwartz B. (2002) Different molecular events account for butyrate-induced apoptosis in two human colon cancer cell lines. (guidetoimmunopharmacology.org)
Caspase-3 activation is a cell requirement during early stages of the skeletal myoblast differentiation. (wikipedia.org)
Caspase signals resulting from the activation of nuclease CAD indicate that the cell differentiation is due to a CAD modification in chromatin structure. (wikipedia.org)
Poly(ADP-ribose) polymerase-1 mediated caspase-independent cell death after ischemia/reperfusion. (bvsalud.org)
At present, the exact mechanisms linking PARP-1 and AIF in the induction of the ROS-induced cell death are still unclear. (bvsalud.org)
Therefore, it appears that further investigations will yield valuable information on underlying mechanisms and potential interventions to reduce caspase -independent cell death during ischemia - reperfusion . (bvsalud.org)
Conclusions: BALB/c mice were more responsive to nanoceria-induced effects, e.g. liver caspase-1 activation, reduced liver vacuolation, and increased spleen cell density. (cdc.gov)

We'll also introduce our new FAM-FLICA WEHD Assay, which allows researchers another target sequence option for detecting caspase-1 in vitro . (immunochemistry.com)

Pro Caspase-3 is inactive and has virtually no activity until it is cleaved by an initiator caspase. (bionotatki.com)

Host-protective caspase-1 activity must be tightly regulated to prevent pathology, but mechanisms controlling the duration of cellular caspase-1 activity are unknown. (altmetric.com)

Detect caspase-1 activity with the FAM FLICA Caspase-1 Assay Kit. (immunochemistry.com)
We recommend using 1 unit per assay for analyzing caspase activity. (enzolifesciences.com)
Additionally rTgPrx treatment reduced caspase-1 activity and IL-1β secretion, while simultaneously increasing IL-10 release. (biomedcentral.com)

The non-canonical string of events is characterized by direct interaction of the other three caspases with Gram-negative bacterial lipopolysaccharide (LPS) with subsequent GSDMD activation. (genetex.com)

Here our experiments show that rTgPrx promotes AAM as indicated by high arginase-1 (arg-1), YM1 and FIZZ expression via both signal transducer and activator of transcription (STAT)6-dependent and -independent mechanisms. (biomedcentral.com)
Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-κB. (nature.com)

Bulk Order Inquiry for FAM FLICA® Caspase-1 (WEHD) Assay Kit ------- (please add any order requirements, including desired quantity, timing, etc. (immunochemistry.com)
ICT is excited to present a webinar on our new product FAM-FLICA Caspase-1 Assay (WEHD)! (immunochemistry.com)

Anatomy24

Organisms5

Diseases3

Chemicals and Drugs119

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes46

Disciplines and Occupations1

Information Science2

Health Care1

Inhibitors9

Antibody9

Cleavage10

Substrate6

Induces2

Inhibitor9

Cysteine4

NLRP36

Pathway11

Inhibition3

Protease2

Pyroptosis7

Recombinant1

Protein7

Activates2

Apoptotic4

Maturation2

Cleaves1

NLRC41

Subunits4

Inhibit4

Tumor necros1

Gene3

Proteins1

Interleukin2

Mechanism4

Subunit1

Substrates1

Inflammasomes5

Activation and Recruitment Domain1

Enzyme activity1

Cells11

Active3

Reagent1

Infection5

Intrinsic1

Cell8

Vitro1

Initiator1

Cellular1

Activity3

Bacterial1

Activator2

WEHD2