Casein kinase 2 alpha. Medical search. Frequent questions

Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. (wikipedia.org)
CK2 (casein kinase 2) is a serine/threonine-selective protein kinase that has been involved in a variety of cellular processes such as DNA repair, cell cycle control and circadian rhythm regulation. (biomedcentral.com)
CK2 (casein kinase 2) is an ubiquitously expressed and highly conserved serine/threonine protein kinase that forms a tetramer containing two catalytic (α and/or α´) subunits and two regulatory β subunits [ 1 ]. (biomedcentral.com)
Casein Kinase 2 Alpha 1 (CKII) is a serine/threonine protein kinase with structural activity. (biomedcentral.com)
Protein kinase CK2 (Casein kinase II) is an ubiquitous and highly conserved protein serine/threonine kinase that is typically found in tetrameric complexes consisting of two catalytic (alpha and/or alpha\') subunits and two regulatory beta subunits . (proteinkinase.biz)
Furthermore, wt-ORF6 leads to phosphorylation of a stress-activated serine/threonine protein kinase, p38 MAPK, suggesting a role of the viral protein in regulating p38 activation. (bvsalud.org)
Here, we report that during intestinal inflammation, multiple inflammatory cytokines promote the activity of a master regulator of cell proliferation and apoptosis, serine/threonine kinase CK2. (diva-portal.org)
AURK significantly promotes activation of IκBα at serine 32, but not serine 36 residues, unlike IκB kinase (IKK) family proteins activate both IκBα serine residues. (researchsquare.com)

[1] [2] It is synthesized primarily as a 155 amino acid polypeptide, resulting in an 18 kDa protein. (wikidoc.org)

Selective inhibitors of protein kinase CK2 with significant cytotoxicity on tumor cells based on a 2-aminothiazole scaffold were described recently. (rcsb.org)
Battistutta R, Sarno S, De Moliner E, Marin O, Issinger OG, Zanotti G, Pinna LA (2000) "The crystal structure of the complex of Zea mays alpha subunit with a fragment of human beta subunit provides the clue to the architecture of protein kinase CK2 holoenzyme" Eur. (proteinkinase.biz)
Benitez MJ, Cochet C, Jimenez JS (2001) "A surface plasmon resonance study of the interactions between the component subunits of protein kinase CK2 and two protein substrates, casein and calmodulin" Mol. (proteinkinase.biz)
Inhibition of protein kinase CK2 by anthraquinone-related compounds. (cathdb.info)

Bidwai AP, Reed JC, Glover CV (1993) "Phosphorylation of calmodulin by the catalytic subunit of casein kinase II is inhibited by the regulatory subunit" Arch. (proteinkinase.biz)

Casein kinase II subunit alpha is an enzyme that in humans is encoded by the CSNK2A1 gene. (wikipedia.org)
The protein encoded by this gene represents the alpha subunit. (wikipedia.org)
The subunit alpha protein activates transcription of the subunit beta gene" J. Biol. (proteinkinase.biz)

Chester N, Yu IJ, Marshak DR (1995) "Identification and characterization of protein kinase CKII isoforms in HeLa cells. (proteinkinase.biz)

The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. (wikipedia.org)
The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. (wikipedia.org)
To increase stability of the holo complex hCK2 holo consist of shortened alpha subunits (aa1-335) and beta subunits. (proteinkinase.biz)
Human recombinant casein kinase 2 holo enzyme (hCK2 holo) containing alpha (aa1-335) and beta subunits expressed separately in E. coli as non-fusion proteins, purified by several chromatography steps (HiTrapQ, Heparin agarose, Superdex200) and reconstituted to the CK2 alpha2beta2 holoenzyme in the course of purification. (proteinkinase.biz)
Since CK2 alpha is unstable and prone to degradation, CK2 subunits alpha was shortened at the C-terminus to obtain a stable, crystallizable alpha subunit. (proteinkinase.biz)
A study with the complete set of subunits (alpha, alpha\' and beta), site-directed autophosphorylation mutants and a bicistronically expressed holoenzyme" Eur. (proteinkinase.biz)

Background: The phosphorylation of NF-kappaB inhibitor alpha (IκBα) protein is pivotal to the regulation of NF-κB transcription factor activity in the cell. (researchsquare.com)

Synthetic peptides including acidic clusters as substrates of yeast casein kinase-2. (cnr.it)

Casein kinase-2 mediates cell survival through phosphorylation and degradation of inositol hexakisphosphate kinase-2. (nih.gov)

This results in inhibition of NFκB activity and the NFκB-dependent expression of anti-apoptotic genes of the Bcl-2 family [ 15 ]. (biomedcentral.com)

Furthermore, when used as a heterologous third dose booster, the RBD-Fc vaccines combined with MF59® increased titres of nAb against other variants including alpha, delta, delta+, gamma, lambda, mu, and omicron BA.1, BA.2 and BA.5. (bvsalud.org)
We obtained 391 sequences and identified 34 Pango lineages including the Alpha, Gamma, Delta, and Omicron variants. (cdc.gov)

Mutations in bone morphogenetic protein receptor 2 ( BMPR2 ) are the cause of most heritable cases but the vast majority of other cases are genetically undefined. (biomedcentral.com)
For example, bone morphogenetic protein receptor type 2 ( BMPR2 ) mutations are observed in 60-80% of familial (FPAH) cases, but data from population registries indicate that penetrance of the disease phenotype ranges from 14 to 42% [ 6 ]. (biomedcentral.com)
METHODS: We report on a protein subunit vaccine comprising the receptor-binding domain (RBD) of the ancestral SARS-CoV-2 spike protein, dimerised with an immunoglobulin IgG1 Fc domain. (bvsalud.org)
It was also shown to act on preosteoblasts - in the form of an increased proliferation - after binding to fibroblast growth factor receptor 1 and activating phosphoinositide 3-kinase . (wikidoc.org)

Some new promising strategies for biomarker discovery include microarray-based profiling at the DNA and mRNA levels, and mass-spectrometry-based profiling at the protein and peptide levels [ 2 ]. (biomedcentral.com)

Description: A sandwich ELISA kit for detection of Protein Kinase C Alpha from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (scalegen.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Protein Kinase C Alpha (PKCa) in serum, plasma, tissue homogenates and other biological fluids. (scalegen.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Protein Kinase C Alpha (PKCa) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. (scalegen.com)

The in vitro treatment of intestinal epithelial cell lines with cytokines resulted in increased CK2 expression and nuclear translocation of its catalytic alpha subunit. (diva-portal.org)

Dobrowolska G, Lozeman FJ, Li D, Krebs EG (1999) "CK2, a protein kinase of the next millennium" Mol. (proteinkinase.biz)

Transmission of SARS-CoV-2 from humans to free-ranging white-tailed deer (Odocoileus virginianus) poses a unique public health risk due to the potential for reservoir establishment where variants may persist and evolve. (cdc.gov)

Whether Vpu can be a virulence element remains to become established but in comparison to HIV-1 carefully related retroviruses such as for example PHA-848125 HIV-2 and SIV that absence expression of a completely functional Vpu proteins also cause much less severe disease results. (cancer-pictures.org)

Basic fibroblast growth factor has been shown to interact with casein kinase 2, alpha 1 , [13] RPL6 [14] and ribosomal protein S19 . (wikidoc.org)

Although antioxidant modulation by SARS-CoV-2 infection has already been described, the viral factors involved in modulating the NRF2 pathway are still elusive. (bvsalud.org)

and 2) the support vector machine with a set of 17 transcripts, such as CCL16 and CDKN2AIP, can yield a leave-one-out cross-validation accuracy of 93.59%, a test accuracy of 76.92%, a large disease-free survival area of 74.81%, and a mean survival prediction error of 3.99 months. (biomedcentral.com)

Inositol hexakisphosphate kinase 2 promotes cell death of anterior horn cells in the spinal cord of patients with amyotrophic lateral sclerosis. (nih.gov)
Inositol hexakisphosphate kinase 2 sensitizes ovarian carcinoma cells to multiple cancer therapeutics. (nih.gov)
Violin plots show distribution of expression levels for Casein kinase I (SMED30025502) in cells (dots) of each of the 12 neoblast clusters. (stowers.org)
Expression of Casein kinase I (SMED30025502) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells. (stowers.org)
Violin plots show distribution of expression levels for Casein kinase I (SMED30025502) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells. (stowers.org)

Newer studies claim that Vpu aswell as the Vpu-like activity PHA-848125 of HIV-2 envelope protein works by overcoming a book species-specific host limitation to HIV launch (51). (cancer-pictures.org)

Casein kinase II subunit alpha is an enzyme that in humans is encoded by the CSNK2A1 gene. (wikipedia.org)
The protein encoded by this gene represents the alpha subunit. (wikipedia.org)
CSNK2A2 is included in Guide to IMMUNOPHARMACOLOGY as it is a subunit of the casein kinase 2 complex. (guidetomalariapharmacology.org)
2. Bischoff N, Olsen B, Raaf J, Bretner M, Issinger OG, Niefind K. (2011) Structure of the human protein kinase CK2 catalytic subunit CK2α' and interaction thermodynamics with the regulatory subunit CK2β. (guidetomalariapharmacology.org)
A ubiquitous casein kinase that is comprised of two distinct catalytic subunits and dimeric regulatory subunit. (nih.gov)
sub:provenance { sub:assertion dcterms:description "[To analyze the prognostic significance of the genes casein kinase 2 alpha subunit (CSNK2A1), anti-apoptosis clone-11 (AAC-11), and tumor metastasis suppressor NME1 in completely resected non-small cell lung cancer (NSCLC) patients. (vu.nl)
At meiosis I, maternal and paternal kinetochores are pulled toward opposite poles, and chiasmata holding bivalent chromosomes together are resolved by cleavage of cohesin's alpha-kleisin subunit (Rec8) along chromosome arms. (nih.gov)
Not only did JQFFP inhibit COX-2 and MMP-9 levels, but it also reduced NF-κB protein expression (to 65% of control) and phosphorylation of its p65 subunit (to 51%) for 100 µM CE. (edu.pl)
Here we show that casein kinase II (CK2) phosphorylates the serine residue (Ser1480) within the C-terminal PDZ ligand (IESDV) of the NR2B subunit of NMDAR in vitro and in vivo . (jneurosci.org)

20. Ricinine: a pyridone alkaloid from Ricinus communis that activates the Wnt signaling pathway through casein kinase 1α. (nih.gov)
1 and 2 (ERK1/2) and AP-1 however not the IκBα-NF-κB signaling pathway. (healthcarecoremeasures.com)
Furthermore Raf and Ras upstream from the ERK1/2 signaling pathway had been mixed up in upregulation of CCL2. (healthcarecoremeasures.com)

A screen profiling 158 kinase inhibitors (Calbiochem Protein Kinase Inhibitor Library I and II, catalogue numbers 539744 and 539745) for their inhibitory activity at 1µM and 10µM against 234 human recombinant kinases using the EMD Millipore KinaseProfiler TM service. (guidetomalariapharmacology.org)
1. Anastassiadis T, Deacon SW, Devarajan K, Ma H, Peterson JR. (2011) Comprehensive assay of kinase catalytic activity reveals features of kinase inhibitor selectivity. (guidetomalariapharmacology.org)
Chen WS, Wei SJ, Liu JM, Hsiao M, Kou-Lin J, Yang WK (2001) Tumor invasiveness and liver metastasis of colon cancer cells correlated with cyclooxygenase-2 (COX-2) expression and inhibited by a COX-2-selective inhibitor, etodolac. (edu.pl)
Figure: Real-time kinetic analysis of kinase inhibitor SB203580 binding to p38α using surface plasmon resonance. (proteinkinase.biz)
2004) "Promyelocytic leukemia is a direct inhibitor of SAPK2/p38 mitogen-activated protein kinase" J Biol Chem. (proteinkinase.biz)
TMCB is a dual-kinase inhibitor of both casein kinase 2 (CK2) and extracellular-signal-regulated kinase 8 (ERK8) with IC50s of 0.50 μM. (csnpharm.com)
PI-828 is a dual PI3K and casein kinase 2 (CK2) inhibitor with IC50s of 173 nM, 149 nM, and 1127 nM for p110α, CK2, and CK2α2 in lipid kinase assay, respectively. (csnpharm.com)
H 89 2HCl is a potent and selective inhibitor of cyclic AMP-dependent protein kinase (protein kinase A) with IC50 of 48 nM. (csnpharm.com)
Ellagic acid is a potent and cell permeable casein kinase 2 (CK2) inhibitor with Ki of 20 nM, acts as a potent antioxidant and anti-mutagenic. (csnpharm.com)
CK1-IN-1 is a casein kinase 1 (CK1) inhibitor extracted from patent WO2015119579A1, compound 1c, has IC50s of 15 nM, 16 nM, 73 nM for CK1δ, and CK1ε, p38σ MAPK, respectively[1]. (csnpharm.com)
BioE-1115 is a highly selective and potent PAS kinase (PASK) inhibitor with an IC50 of ~4 nM. (csnpharm.com)

A screen of 72 inhibitors against 456 human kinases. (guidetomalariapharmacology.org)
A screen profiling the inhibitory activity of 178 commercially available kinase inhibitors at 0.5µM against a panel of 300 recombinant protein kinases using the Reaction Biology Corporation Kinase Hotspot SM platform. (guidetomalariapharmacology.org)
Cerella C, Sobolewski C, Dicato M, Diederich M (2010) Targeting COX-2 expression by natural compounds: a promising alternative strategy to synthetic COX-2 inhibitors for cancer chemoprevention and therapy. (edu.pl)
We provide our Kinascreen services to kinetically characterize small molecule kinase inhibitors for all p38 isoforms (p38α, p38β, p38γ and p38δ) in their phosphorylated (activated) and unphosphorylated (non-activated) state. (proteinkinase.biz)

3. Casein kinase 1α regulates an MDMX intramolecular interaction to stimulate p53 binding. (nih.gov)
5. Casein kinase 1α regulates murine spermatogenesis via p53-Sox3 signaling. (nih.gov)
Interestingly, activity of the NMDAR and Ca 2+ /calmodulin-dependent protein kinase II regulates CK2 phosphorylation of Ser1480. (jneurosci.org)

Speculatively,CSNK2A2 ligands such as silmitasertib or TMCB could be utilised to examine the effect of inhibiting the CSNK2A2-N protein-protein interaction on SARS-CoV-2 pathobiology. (guidetomalariapharmacology.org)

We show here that multiple phosphorylation sites within Rec8 as well as two different kinases, casein kinase 1delta/epsilon (CK1delta/epsilon) and Dbf4-dependent Cdc7 kinase (DDK), are required for Rec8 cleavage and meiosis I nuclear division. (nih.gov)
An evaluation of polymorphisms in casein kinase 1 delta and epsilon genes in major psychiatric disorders. (cdc.gov)

12. Casein kinase 1 α phosphorylates the Wnt regulator Jade-1 and modulates its activity. (nih.gov)

Suitable for kinase molecule interaction analyses using SPR , as substrate for kinase activity assays and for western blot analyses. (proteinkinase.biz)

Kyriakis JM, Avruch J. (2001) „Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation. (proteinkinase.biz)

1. MDMX inhibits casein kinase 1α activity and stimulates Wnt signaling. (nih.gov)
7. Casein kinase 1α inhibits p53 downstream of MDM2‑mediated autophagy and apoptosis in acute myeloid leukemia. (nih.gov)
13. Calotropin: a cardenolide from calotropis gigantea that inhibits Wnt signaling by increasing casein kinase 1α in colon cancer cells. (nih.gov)
These results provide the first evidence that JQFFP inhibits COX-2, MMP-9, and NF-κB expression, which may suggest it has cytotoxic, anti-inflammatory, and anti-metastatic activities towards colon cancer SW-480 cells. (edu.pl)

The p38 MAPK (mitogen-activated protein kinase) pathway contains protein kinases which engage in respond to various extracellular stimuli an intracellular signalling cascade, coordinating the activation of gene transcription, protein synthesis, cell cycle machinery, cell death, and differentiation. (proteinkinase.biz)
Roux PP, Blenis J.,(2004) "ERK and p38 MAPK-activated protein kinases: a family of protein kinases with diverse biological functions. (proteinkinase.biz)

Furthermore Ras Raf MEK extracellular signal-regulated kinase 1 and 2 (ERK1/2) and AP-1 are straight involved with SARS-CoV-induced CCL2 upregulation. (healthcarecoremeasures.com)

18. Small-molecule inhibition of Wnt signaling through activation of casein kinase 1α. (nih.gov)

2. Regulation of p53-MDMX interaction by casein kinase 1 alpha. (nih.gov)

The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. (wikipedia.org)

A6: Cyclin B/Cdk1, a cell cycle-dependent kinase, is capable of phosphorylating SIRT1 at T530 and S540. (nih.gov)

Phosphorylation of Nrf2 in the transcription activation domain by casein kinase 2 (CK2) is critical for the nuclear translocation and transcription activation function of Nrf2. (cdc.gov)
Chao CC;Ma YL;Lee EHY*, 2011, 'Brain-Derived Neurotrophic Factor Enhances Bcl-xL Expression Through Protein Kinase Casein Kinase 2-Activated and Nuclear Factor Kappa B-Mediated Pathway in Rat Hippocampus, ' Brain Pathology, Vol.21, No.2, pp.150-162. (nccu.edu.tw)

TNF-alpha polymorphisms in chronic beryllium disease and beryllium sensitization. (cdc.gov)

In this study, we utilized a novel chimera mouse that lacks the expression of BM beta1/2 adrenergic receptors (b1/2-ARs) to investigate the role of the sympathetic drive to the BM in gut and microbiota homeostasis. (nih.gov)
Charalambous MP, Lightfoot T, Speirs V, Horgan K, Gooderham NJ (2009) Expression of COX-2, NF-kappaB-p65, NF-kappaB-p50 and IKKalpha in malignant and adjacent normal human colorectal tissue. (edu.pl)
Dixon DA, Blanco FF, Bruno A, Patrignani P (2013) Mechanistic Aspects of COX-2 Expression in Colorectal Neoplasia. (edu.pl)
Human casein alpha s1 induces proinflammatory cytokine expression in monocytic cells by TLR4 signaling. (monocyte.eu)
SCOPE: Human casein alpha S1 (CSN1S1) is a milk-derived protein, which gives rise to sustained antibody formation after breast feeding in infancy and induces the expression of proinflammatory cytokines. (monocyte.eu)

6. Suppression of casein kinase 1alpha in melanoma cells induces a switch in beta-catenin signaling to promote metastasis. (nih.gov)

16. Casein Kinase 1α as a Regulator of Wnt-Driven Cancer. (nih.gov)

17. Basic region of residues 228-231 of protein kinase CK1alpha is involved in its interaction with axin: binding to axin does not affect the kinase activity. (nih.gov)

To our knowledge, this study is the first to suggest a key role of BM b1/2-ARs signaling in host-microbiota interactions, and reveals specific molecular mechanisms that may lead to generation of novel anti-inflammatory treatments for many immune and autonomic diseases as well as gut dysbiosis across the board. (nih.gov)

Anatomy19

Organisms11

Chemicals and Drugs161

Analytical, Diagnostic and Therapeutic Techniques and Equipment18

Phenomena and Processes42

Disciplines and Occupations1

Technology, Industry, Agriculture4

Information Science3

Serine8

Polypeptide1

Protein kinase CK24

Catalytic subunit1

Gene3

CKII1

Subunits6

Inhibitor1

Substrates1

Mediates1

Genes1

Gamma2

Receptor4

Peptide1

Human3

Translocation1

19991

Variants1

Proteins1

Interact1

Modulation1

Survival1

Cells5

Species1

Subunit9

Signaling pathway3

Inhibitor11

Inhibitors4

Regulates3

CSNK2A21

Epsilon2

Phosphorylates1

Substrate1

Signal transduc1

Inhibits4

MAPK2

Extracellular1

Small molecule1

Regulation1

Catalytic activity1

Cell Cycle1

Nuclear2

Polymorphisms1

Expression5

Induces1

Regulator1

Axin1

Interactions1