AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, Agriculture
CartilageCartilage, ArticularHyaline CartilageTarsal JointsFibrocartilageMandibular CondyleChondrocytesHydroxylysineTemporomandibular JointCartilage DiseasesFibrillar CollagensGlycosaminoglycansTendonsProteoglycansGrowth PlateCollagenChick EmbryoNasal CartilagesBone and BonesGlucosamineOsteoarthritisEar CartilageLaryngeal CartilagesTissue EngineeringTissue ScaffoldsKnee JointCells, CulturedCartilage Oligomeric Matrix ProteinOsteoarthritis, KneeAggrecansCell DifferentiationCollagen Type IIMatrilin ProteinsChondrogenesisExtracellular Matrix ProteinsFractures, CartilageStiflePatellaTibiaFemurMenisci, TibialNasal SeptumEpiphysesArytenoid CartilageCricoid CartilageCattleThyroid CartilageOsteochondritisMatrix Metalloproteinase 13Weight-BearingJointsCompressive StrengthHyaluronic AcidFemur HeadSynovial MembraneExtracellular MatrixMagnetic Resonance ImagingSynovial FluidStress, MechanicalChondroitin SulfatesBiomechanical PhenomenaKnee InjuriesArthritis, ExperimentalLubricationSOX9 Transcription FactorProcollagen N-EndopeptidaseUronic AcidsAnterior Cruciate LigamentArthroplasty, SubchondralMatrix Metalloproteinase 3Microscopy, PolarizationTissue Culture TechniquesInjections, Intra-ArticularCollagen Type IXRibsChondroitinCollagenasesLectins, C-TypeBone DevelopmentFrictionRabbitsCollagen Type XHumerusArthroscopyOsteochondrodysplasiasRhinoplastyOsteogenesisCalcification, PhysiologicCollagen Type XIJoint DiseasesIoxaglic AcidPeriosteumHindlimbArthritisElastic CartilageChondroitin Sulfate ProteoglycansChondrosarcomaMatrix MetalloproteinasesInterleukin-1Glycoproteins
RegenerationRegenerative MedicineArticular cartilage repairChondrocytesTissuesStem cellKneeBone marrowDefectsHealthy cartilageRegenerate cartilageLesionsPlatelet-rich plasmaScaffoldsMSCsRepair cartilageNative cartilageArthritisConnective tissueConstructsPhenotypeHypothesisTransplantationHyaline-like cartilageStimulateDifferentiationInvolving cartilage developmentExplantsExtracellularInjuries and cartilageTissue engineered cartilageClinicalTherapiesJointsProgenitor cellsOsteoarthritic cartilageLoss of proteoglycanProduce new cartilageFunctionalCollagenHypoplasiaType of cartilageResponse of cartilageAdipose tissue-derived stem cellsProgressionInjuryDegenerationDamageMechanicalAvascular tissueMicrofractureNormal cartilageBonesVitro cartilageLost cartilage
Regeneration28
Regenerative Medicine12
  • Our group of scientists and clinicians has been continuously funded by the California Institute of Regenerative Medicine (CIRM) to develop a first-in class pluripotent stem cell-based therapy for focal articular cartilage lesions. (ca.gov)
  • 1. Cell Therapy and Regenerative Medicine Unit, Rheumatology Group, Institute of Biomedical Research of A Coruña (INIBIC), University Hospital Complex A Coruña (CHUAC), Galician Health Service (SERGAS), Department of Medicine, Faculty of Health Sciences, University of A Coruña, A Coruña, Spain. (medsci.org)
  • Combining biomimetic regenerative medicine strategies will allow us to improve tissue-engineered auricular cartilage with respect to biochemical composition and functionality, as well as microstructural organization and overall shape. (biomedcentral.com)
  • We will use the lessons learnt from auricular cartilage tissue engineering to illustrate how combining additive manufacturing and regenerative medicine for tissue-engineering purposes can be used to create functional and durable tissue with potential to shift the paradigm in reconstructive surgery. (biomedcentral.com)
  • at the Wake Forest Institute of Regenerative Medicine examined cartilage deterioration in patients with osteoarthritis. (forbes.com)
  • It is a type of regenerative medicine used to repair damaged cells in the body. (specialtycareclinics.com)
  • This study demonstrates the superiority compared to conventional bulk hydrogel with encouraging potential of this system to be applied in the further cartilage tissue clinical translate studies, with additional potential for a broad range of regenerative medicine. (monash.edu)
  • Therefore, increasing epidemiology of such diseases require effective treatment options, generating significant demand for regenerative medicine used to restore damaged cartilage. (radiantinsights.com)
  • Although SYNAPSE VINCENT can only analyze the thickness of cartilage, and the reproducibility of the error is debatable, SYNAPSE VINCENT would be useful as a clinical tool for regenerative medicine. (biomedcentral.com)
  • However, regenerative medicine cell therapy, including platelet-rich plasma (PRP) therapy, has recently become widespread and gathered attention as the third type of treatment. (biomedcentral.com)
  • Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs). (swan.ac.uk)
  • The study, led by faculty at The Forsyth Institute, suggests a new approach for making cartilage cells with huge implications in regenerative medicine for future cartilage injuries and degeneration treatments. (drkayvanhaddadan.com)
Articular cartilage repair3
  • Articular cartilage repair:​ basic science and clinical progress. (lievers.net)
  • This article reviews biological treatment options for articular cartilage repair, including platelet-rich plasma (PRP), bone marrow aspirate concentrate (BMAC) and cell-based therapies. (jorgechahlamd.com)
  • Final considerations: Current treatments for articular cartilage repair have major limitations. (bvsalud.org)
Chondrocytes21
  • Surgical techniques currently being studied include: bone marrow stimulation osteochondral autograft transplantation osteochondral allograft transplantation autologous chondrocyte implantation cell-based (using chondrocytes or stem-cells) or acellular scaffolding Articular cartilage stem cell paste grafting is another technique in use and is undergoing continued investigation. (wikipedia.org)
  • Using this marker, the researchers found that OCR cells self-renew and generate key bone and cartilage cells, including osteoblasts and chondrocytes. (sciencedaily.com)
  • CaMKII inhibition in human primary and pluripotent stem cell-derived chondrocytes modulates effects of TGFbeta and BMP through SMAD signaling. (ca.gov)
  • Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes. (ca.gov)
  • Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes. (ca.gov)
  • The response of these cells to both mechanical and inflammatory stimuli was found to be similar to that of native chondrocytes, displaying the usability of this cell line as a model for cartilage tissue engineering. (wustl.edu)
  • In healthy cartilage, the protein lubricin is produced by the superficial zone chondrocytes and plays a role in both the mechanics of joint motion as well as having an anti-inflammatory effect. (wustl.edu)
  • Cartilage is the slippery "anatomical Teflon" secreted by specialised cells called chondrocytes. (thenakedscientists.com)
  • This was treated with enzymes to release the cartilage-producing chondrocytes which were then incubated in a culture vessel containing a suitable growth medium for fourteen days to increase their numbers. (thenakedscientists.com)
  • Chondrocytes begin to elaborate destructive enzymes causing cracks and fissures in the cartilage. (medicaltourism.video)
  • Autologous chondrocytes implantation and osteochondral autograft transplantation are limited by scarce cartilage production, low proliferative capacity of chondrocytes, chondrocyte de-differentiation and complications due to donor site morbidity 5 . (nature.com)
  • MSCs can be differentiated along different cell lineages of mesodermal origin including osteoblasts, chondrocytes, skeletal myocytes or visceral stromal cells 9 . (nature.com)
  • This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic di erentiation. (swan.ac.uk)
  • Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties. (swan.ac.uk)
  • has shown promising Moxonidine Hydrochloride results for development of designed cartilage using animal chondrocytes and Moxonidine Hydrochloride stem cells (O'Connell using chondrocytes and stem cells derived from numerous animal models (Farrell functional cartilage tissue development of non-human mammalian cells encapsulated in agarose (Sampat (2012). (biongenex.com)
  • Human chondrocytes were cultured in growth medium made up of 1 ng/ml TGFβ1 10 ng/ml PDGFββ and 5 ng/ml FGF2 to primary the cells for 3D cultures. (biongenex.com)
  • To understand the mechanism of cartilage degeneration and remodeling, it is important to know the mechanical stimuli to chondrocytes for physiological loading conditions. (cdc.gov)
  • Background: Chondrocytes are the primary cells responsible for maintaining cartilage integrity and function. (lu.se)
  • Developing an accessible and cost-effective model to generate viable chondrocytes and to assess their response to different bioactive compounds can significantly advance our knowledge of cartilage biology and contribute to the discovery of novel therapeutic approaches. (lu.se)
  • Objective: We developed a novel, streamlined protocol for generating chondrocytes from bone marrow-derived mesenchymal stem cells (BMSCs) in a 3D culture system that offers. (lu.se)
  • Objective: We developed a novel, streamlined protocol for generating chondrocytes from bone marrow-derived mesenchymal stem cells (BMSCs) in a 3D culture system that offers significant implications for the study of cartilage biology and the discovery of potential therapeutic interventions for cartilage-related and associated disorders. (lu.se)
Tissues16
  • Autoimmune disorders, which occur when the immune system malfunctions and attacks the body's tissues and organs, occur in some people with cartilage-hair hypoplasia. (medlineplus.gov)
  • However, aside from muscle development, the roles of ESRRs in other tissues including bone and cartilage have just begun to be investigated 10 . (nature.com)
  • One of the body's tissues that cannot naturally regenerate is cartilage. (specialtycareclinics.com)
  • Although MF-activated SSCs tended to form fibrous tissues, localized co-delivery of BMP2 and soluble VEGFR1 (sVEGFR1), a VEGF receptor antagonist, in a hydrogel skewed differentiation of MF-activated SSCs toward articular cartilage. (howhelp.org)
  • The adult body has a small number of stem cells in many tissues. (medicaltourism.video)
  • This mouse model provides critical answers to important questions concerning the vascularization of engineered tissue, which offers a viable option for repairing defects, especially when the desired amount of autologous cartilage or other tissues is not available and the nutritive situation at the implantation site is poor. (nih.gov)
  • The success of any technology aimed at repairing chondral defects will thus be based on its ability to produce tissues that most closely recapitulate the mechanical and biochemical properties of native cartilage. (nature.com)
  • However, the quality of neo-tissues resulting from these cell types is inadequate due to ine cient chondrogenic di erentiation and endochondral ossi cation, respectively. (swan.ac.uk)
  • Blastemal cells have been shown to originate either from dedifferentiated tissues or from existing progenitor cells in various species, but their origin has not been determined in lizards. (pitt.edu)
  • The contribution of muscle cells to regenerated tail tissues was evaluated using muscle creatine kinase promoter-driven Cre recombinase in conjunction with the Cre-responsive green-to-red fluorescence shift construct CreStoplight. (pitt.edu)
  • 21 days after amputation, tail tissues were analyzed by histology for red fluorescent protein (RFP)-positive cells.ResultsAt 7 days post-amputation, Dil-labeled cartilage cells localized to the subapical space contributing to the blastema. (pitt.edu)
  • Lineage tracing of myocytes showed colocalization of RFP with Col2 and MHC in differentiated tissues at 21 days post-amputation.ConclusionThis study demonstrates that differentiated cartilage cells contribute to both regenerated muscle and cartilage tissues following tail loss, and in turn, differentiated muscle cells contribute to both tissue types as well. (pitt.edu)
  • COMP is not only cartilage-derived but is common in other tissues, such as synovium and tendon. (prospecbio.com)
  • Ultrasound: Using radio waves, this test can be used to show the extent of a cartilage injury as well as other tissues in the knee. (uhhospitals.org)
  • PCB methyl sulphones and related compounds: Identification of target cells and tissues in different species. (cdc.gov)
  • The transplantation of human tissues, organs or cells is an established form of treatment that has been acknowledged as the best and very often only life-saving therapy for several serious and life-threatening congenital, inherited and acquired diseases and injuries. (who.int)
Stem cell33
  • citation needed] Published descriptions of the articular cartilage stem cell paste grafting technique are readily available. (wikipedia.org)
  • A stem cell capable of regenerating both bone and cartilage has been identified in bone marrow of mice. (sciencedaily.com)
  • Previously, Dr. Wang found an analogous stem cell in the intestinal tract and observed that it was also abundant in the bone. (sciencedaily.com)
  • Laughlin, M. and Lazarus, H. (2010) Allogeneic Stem Cell Transplantation. (scirp.org)
  • Sorensen, M. (2008) Stem Cell Applications in Diseases. (scirp.org)
  • Hypothesis: The harvest technique of BM may highly influence stem cell heterogeneity and, thus, cartilage formation because these cells have distinct spatial localization within BM from the same bone. (whiterose.ac.uk)
  • By improving stem cell-based cartilage repair with interleukin-37 (IL37), we might be able to inhibit the worsening progression of focal cartilage defects and prevent further development of joint diseases such as osteoarthritis. (ru.nl)
  • Stem cell therapy is used for lowering inflammation and controlling the immune system. (specialtycareclinics.com)
  • This fact makes stem cell therapy an effective remedy for a number of illnesses. (specialtycareclinics.com)
  • Stem cell therapies have also been used for the treatment of autoimmune, inflammatory, neurological, orthopaedic, and traumatic disorders. (specialtycareclinics.com)
  • While new stem cell therapies don't always cure these conditions, the premise is to allow the body to heal itself well enough to reduce the symptoms of the requirements for a long period of time. (specialtycareclinics.com)
  • Most patients only need a short period of recovery time following their stem cell injection, which may be administered under local anaesthetic. (specialtycareclinics.com)
  • Stem cell injections treatment for joint pain due to cartilage damage can decrease pain and inflammation while improving mobility. (specialtycareclinics.com)
  • If you have joint pain caused by cartilage loss or damage, stem cell treatment may be the right option for you. (specialtycareclinics.com)
  • Our doctors are well-trained and experienced in giving stem cell injection treatment. (specialtycareclinics.com)
  • Dr. Bennett was using these techniques before the wider medical community understood their true usefulness (over 16 years for PRP/stem cell procedures) - and the clinical research has shown that he made the right choice. (bennettorthosportsmed.com)
  • Fortunately, recent research into stem cell therapy has shown it is an effective way to help people suffering from damaged cartilage. (r3stemcell.com)
  • Here we investigate the ability of resident skeletal stem-cell (SSC) populations to regenerate cartilage in relation to age, a possible contributor to the development of osteoarthritis5,6,7. (howhelp.org)
  • These data indicate that following MF, a resident stem-cell population can be induced to generate cartilage for treatment of localized chondral disease in OA. (howhelp.org)
  • Dr. Struhl then suggested another option to help regrow the cartilage … stem cell therapy , and hopefully it could spare her the intensity of the patellofemoral maltracking surgery. (shouldersandknees.com)
  • He explained how stem cell therapy actually helped his situation with his own knee to regrow cartilage. (shouldersandknees.com)
  • Extremely impressed that he practiced what he preaches, Rachel made the decision with her husband to try the post-op stem cell therapy treatment first. (shouldersandknees.com)
  • Rachel is running again 4.5 months after the microfracture knee surgery and 3.5 months after the stem cell therapy. (shouldersandknees.com)
  • In this video you will hear testimonial of patients stem cell therapy for knee carrtilage and the results of ACL repair. (medicaltourism.video)
  • We purified and cultured adipose-derived mesenchymal stem cells and then performed adipose-derived mesenchymal stem cell therapy by directly injecting these cells into the articular cartilage. (biomedcentral.com)
  • We have shown in this case report the promising effects of adipose-derived stem cell intraarticular injections in treating osteoarthritis and the use of new diagnostic instruments. (biomedcentral.com)
  • As a method of using somatic stem cells, we perform adipose-derived mesenchymal stem cell (ASC) therapy in the hospital, where adipose-derived mesenchymal stem cells (MSCs) are purified, cultured, and injected into the joint. (biomedcentral.com)
  • Transplantation of ASCs is one of mesenchymal stromal stem cell-based therapies. (biomedcentral.com)
  • Stem cell-based approaches are also being actively pursued in hopes of improved outcome. (nature.com)
  • Mesenchymal stem cells (MSCs) are one of the most thoroughly studied and understood stem cell types. (lifeboat.com)
  • He is also an affiliate faculty member of the Harvard Stem Cell Institute. (drkayvanhaddadan.com)
  • GATA3 is a game changer because we can use it to potentially change any somatic cell to become a cartilage-forming cell, similar to using four stem cell factors to generate embryonic stem cell-like cells called induced pluripotent stem cells (iPSC). (drkayvanhaddadan.com)
  • Haematopoietic stem cell transplants have been performed in more than 1 500 000 patients (both autologous and allogeneic) to date.1 Although haematological cancers remain the main indication, haematopoietic stem cell transplants are increasingly considered in the treatment of non-malignant disorders and genetic diseases such as haemoglobinopathies (sickle cell anaemia, thalassaemia) that can benefit greatly from this type of transplant. (who.int)
Knee29
  • Articular cartilage helps the knee move freely. (healthline.com)
  • Sports injuries, accidents, and years of normal wear and tear can damage both types of cartilage in the knee, making it necessary to repair or replace the lost cartilage. (healthline.com)
  • Some long-standing approaches include surgery to repair torn cartilage or knee joint replacement. (healthline.com)
  • In this article, we review traditional and newly emerging approaches to knee cartilage replacement and repair. (healthline.com)
  • If the loss of knee cartilage and symptoms are minor, you may be able to put off surgery or other interventions through physical therapy . (healthline.com)
  • In this procedure, tiny holes are drilled in one or more bones in the knee joint to stimulate greater blood flow and the release of cells that form new cartilage. (healthline.com)
  • It starts with an arthroscopic cartilage biopsy of the knee. (healthline.com)
  • Also called a mosaicplasty, this surgery uses healthy cartilage from elsewhere in the knee to replace cartilage that has significantly worn away. (healthline.com)
  • Knee-d new cartilage? (thenakedscientists.com)
  • Patients with knee injuries have been treated using cartilage grown from their own noses, Swiss doctors announced this week. (thenakedscientists.com)
  • Under local anaesthetic, and using standard knee operating techniques, the patches were implanted into the damaged knee joint, "butter side down" so that the new cells were in contact with the underlying bone at the injury site. (thenakedscientists.com)
  • Our findings confirm the safety and feasibility of cartilage grafts engineered from nasal cells to repair damaged knee cartilage. (thenakedscientists.com)
  • Knee Cartilage Damage? (bennettorthosportsmed.com)
  • articular cartilage which is the gliding surface on the end of the bones and two pads called the meniscus in each knee. (bennettorthosportsmed.com)
  • It promotes the secretion of synovial fluid from synovial cells, lubricate, absorb shock and nourish the knee joint. (yumtrade.com)
  • 14.Steadman, J. R., Rodkey, W. G., Briggs, K. K. & Rodrigo, J. J. [The microfracture technic in the management of complete cartilage defects in the knee joint]. (howhelp.org)
  • The mixture of stem cells and hyaluronic acid was transplanted into knees of miniature pigs that had sustained knee injuries. (supergene.com)
  • Mesenchymal stem cells from cord blood are readily obtained and can be stored long-term, thus providing an attractive alternative to conventional knee and hip replacement surgeries. (supergene.com)
  • Knee implants that generate a tiny electrical current may be able to stimulate cartilage regrowth as a treatment for arthritis. (newscientist.com)
  • Some research suggests that a mild electric current can encourage cartilage cells in the knee to multiply and repair damage . (newscientist.com)
  • Nguyen's group tested the current idea by creating holes in the knee cartilage of rabbits and patching them up with the material. (newscientist.com)
  • Subfracture insult to a knee joint causes alterations in the bone and in the functional stiffness of overlying cartilage. (lievers.net)
  • Based on this review, few studies have reported outcomes of these treatment options in the management of knee and hip articular cartilage damage, although generally positive outcomes are reported. (jorgechahlamd.com)
  • Our goal is your goal - to minimize your knee cartilage injury symptoms and allow you to return to your active lifestyle. (uhhospitals.org)
  • Having a cartilage tear in the knee causes pain that can impact your day-to-day life. (uhhospitals.org)
  • When surgery is the best treatment path for a cartilage tear knee injury, orthopedic surgeons and sports medicine physicians at University Hospitals collaborate to determine the best option for each patient's individual goals. (uhhospitals.org)
  • Building knee cartilage has been a dream of researchers, and now several methods are under study. (medscape.com)
  • The cartilage becomes thicker, and it will do a better job of shock absorption and it will slow the progression of already established knee OA," he says. (medscape.com)
  • With the treatment, he says, you restore the normal equilibrium in the knee by protecting it from cartilage breakdown. (medscape.com)
Bone marrow10
  • Oct. 8, 2021 Researchers have identified a subpopulation of mesenchymal stem cells in the bone marrow that express the marker CD73. (sciencedaily.com)
  • Jan. 29, 2020 Scientists used molecular motors to manipulate the protein matrix on which bone marrow-derived mesenchymal stem cells are grown. (sciencedaily.com)
  • The purpose of this study was to investigate cartilage repair of in vitro lesion models using human bone marrow mesenchymal stromal cells (hBMSCs) with different collagen (Col) scaffolds. (medsci.org)
  • Background: Connective tissue progenitors (CTPs) from native bone marrow (BM) or their culture-expanded progeny, often referred to as mesenchymal stem/stromal cells, represents a promising strategy for treatment of cartilage injuries. (whiterose.ac.uk)
  • Commonly used cell sources for tissue engineering are adipose-derived and bone marrow-derived mesenchymal stem cells (ASCs and MSCs) because they can be patient matched, expand rapidly in culture, and have multipotent differentiation potential. (wustl.edu)
  • Fatty (adipose) tissue, bone marrow, synovial tissue as well as other parts of the body contains stem cells. (specialtycareclinics.com)
  • The body's damaged or missing cartilage in joints can be repaired using stem cells that have been extracted from a patient's bone marrow or adipose tissue, and then concentrated and sterilised. (specialtycareclinics.com)
  • The cell source was obtained from bone marrow specimens from healthy human subjects undergoing hip replacement surgery. (lievers.net)
  • Stem cells from bone marrow are recruited to the scaffold," says Nguyen. (newscientist.com)
  • In addition to the basic structural role , the larger bones in the body contain bone marrow, where blood cells are formed. (cdc.gov)
Defects13
Healthy cartilage2
  • Healthy cartilage is essential to the proper functioning of joints within the body. (wikipedia.org)
  • The first study found that the use of mesenchymal stem cells (MSCs) were equally as effective as implanting healthy cartilage tissue in affected areas, and the effectiveness persisted for at least two years following treatment. (bennettorthosportsmed.com)
Regenerate cartilage6
Lesions4
  • The work described in this proposal is designed to produce a universal treatment for articular cartilage lesions. (ca.gov)
  • Lesions were made in human cartilage biopsies. (medsci.org)
  • Furthermore, IL1β pre-treated biopsies decreased capacity for repair by hBMSCs and decreased levels of chondrogenic phenotype of human cartilage lesions. (medsci.org)
  • Articular cartilage damage is seen most commonly in focal chondral lesions and early osteoarthritis and is a significant concern in the orthopedic community. (jorgechahlamd.com)
Platelet-rich plasma4
  • At present, treatment options for cartligage damage are very limited, ranging from trying to stimulate injured cartilage to regrow by injecting growth-factor and platelet-rich plasma, to grafting pieces of cartilage from other joints or even completely replacing a joint with a prosthesis. (thenakedscientists.com)
  • Combining MSCs with autologous growth factors found in platelet-rich plasma also has added a boost to the natural abilities of stem cells to multiply and divide. (medicaltourism.video)
  • Once the irritation has been completed, stem cells as well as the platelet rich plasma are injected into the prepared area. (medicaltourism.video)
  • The growth factors within the platelet rich plasma act on receptors found on the surface of stem cells and cause the stem cells to differentiate and multiply. (medicaltourism.video)
Scaffolds12
  • In the near future, surgeons will be able to impregnate custom-designed scaffolds with cartilage-forming stem cells and chemicals that stimulate their growth and then implant them into patients during a single procedure, the researchers said. (neatorama.com)
  • Data showed that hBMSCs cultured only on Col I +Col II +HS scaffolds displayed a chondrocyte-like morphology and cartilage-like matrix close to native cartilage. (medsci.org)
  • Díaz-Prado, S.M. Human Cartilage Engineering in an In Vitro Repair Model Using Collagen Scaffolds and Mesenchymal Stromal Cells. (medsci.org)
  • We hope to achieve this by creating a tissue replacement product through tissue engineering using an abundant cell source of mesenchymal stems cells seeded in Collagen Type I scaffolds, and treated not only with chondrogenic growth media in culture, but three different stimulation algorithms to improve structural and functional properties. (lievers.net)
  • Cells were expanded, seeded on collagen type I scaffolds and grown statically for 1 week. (lievers.net)
  • In this study, polycaprolactone (PCL)-based polyurethane scaffolds are seeded with 1 × 10(6) human cartilage cells and implanted in the right hind leg of a nude mouse using an arteriovenous flow-through vessel loop for angiogenesis for the first 3 weeks. (nih.gov)
  • Presented by Laila Montaser, Medicine Menoufia University Our objective is to construct engineered biomaterial scaffolds to advocate cell culture and create novel cartilage tissue. (thermofisher.com)
  • This manuscript displays existent notions and schemas in cartilage engineering with a confirmation on the employment of stem cells and nanotechnology in the output of biomimetic cartilage reconditioned scaffolds. (thermofisher.com)
  • The same donor cells were expanded for 3D cultures in pellets and agarose scaffolds. (biongenex.com)
  • Objective: To review the literature regarding sources of cells, scaffolds, and morphogenic agents currently used to produce articular cartilage. (bvsalud.org)
  • In this context, tissue engineering is an emerging and multidisciplinary field, which three main components are responsive cells, scaffolds, and morphogenic agents. (bvsalud.org)
  • However, the triad components - stem cells, morphogenic agents, and scaffolds - need further improvement before clinical application. (bvsalud.org)
MSCs10
  • The study also showed that the adult OCRs are distinct from mesenchymal stem cells (MSCs), which play a role in bone generation during development and adulthood. (sciencedaily.com)
  • Researchers presumed that MSCs were the origin of all bone, cartilage, and fat, but recent studies have shown that these cells do not generate young bone and cartilage. (sciencedaily.com)
  • The CUMC study suggests that OCR stem cells actually fill this function and that both OCR stems cells and MSCs contribute to bone maintenance and repair in adults. (sciencedaily.com)
  • In addition to creating a model cell line, we also wanted to understand how MSCs change with passage and why that limits their differentiation at later passages. (wustl.edu)
  • For this purpose, we used single cell RNA sequencing to investigate the transcriptome of MSCs from three donors at a single cell resolution over the course of eight passages. (wustl.edu)
  • As these MSCs were not sorted at harvest, we expected there to be a heterogenous population of cells at the earlier passages, which was not found. (wustl.edu)
  • He is constantly researching and developing new ways to help his patients and has been on the forefront of using MSCs to treat damaged cartilage and tissue. (bennettorthosportsmed.com)
  • Mesenchymal stem cells (MSCs) support chondrogenic differentiation and are an attractive cell source for cartilage tissue engineering. (nature.com)
  • Today, we will be taking a look at MSCs and a new human clinical trial focused on treating osteoarthritis, an age-related inflammatory condition that leads to the breakdown of bone and cartilage. (lifeboat.com)
  • Recently, a subpopulation of stem/progenitor cells has been identi ed within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored. (swan.ac.uk)
Repair cartilage1
  • I have nightmares that six months after I finally break down and get all my joints replaced, they'll announce that they can repair cartilage with a pill or something. (neatorama.com)
Native cartilage1
  • In laboratory tests, the fabric scaffold that the researchers have created had the same mechanical properties as native cartilage. (neatorama.com)
Arthritis5
Connective tissue5
  • Articular cartilage is a connective tissue overlying the ends of bones that provides smooth joint surfaces. (wikipedia.org)
  • Cartilage is considered a connective tissue and is at its most abundant during infancy. (r3stemcell.com)
  • Osteoarthritis is a bone disease caused by the breakdown of cartilage, the connective tissue found between bones. (supergene.com)
  • They then supplemented the stem cells with a gel containing hyaluronic acid, a type of molecule normally found in cartilage and connective tissue. (supergene.com)
  • however, significant vacuolization is present in the heart's connective tissue cells of the heart valves. (medscape.com)
Constructs5
  • However, a lack of sufficient nutrient supply to cartilage constructs limits this procedure. (nih.gov)
  • The amount of cells increased in vascularized constructs compared to the controls but at the same time noticeably less extracellular matrix was produced. (nih.gov)
  • These results underscored the AuCPCs' tissue-speci c di erentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs. (swan.ac.uk)
  • These results suggest that combined use of micro-space culture and mechanical stimuli facilitates hybrid bone/cartilage tissue formation from iPSCs, and that the bone/cartilage tissue ratio in iPSC constructs could be manipulated through the induction protocol. (elsevierpure.com)
  • However engineered cartilage development within agarose was better for constructs cultured with TGFβ3. (biongenex.com)
Phenotype4
  • [ 15 ] The second consists of single nucleotide substitutions and other changes that involve at most 2 nucleotides in highly conserved regions of the gene.These are considered leaky mutations and result in variable expression of the gene, which may explain the variable phenotype seen in cartilage-hair hypoplasia. (medscape.com)
  • These latter mutations result in variable expression of the gene, which may explain the variable phenotype seen in cartilage-hair hypoplasia. (medscape.com)
  • Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle. (swan.ac.uk)
  • To correlate chondrogenic phenotype with EF-induced migration direction cells from passages that were directed to the cathode (P2) and anode (P3) were placed in pellet culture and aggrecan expression was measured after 7 days (observe section on 3D cultures below for culture details). (biongenex.com)
Hypothesis3
  • The hypothesis was that harvesting a mixture of articular cartilage and cancellous bone would combine pluripotent cells of the cancellous bone with cartilage extracellular matrix and growth factors and when exposed to continuous motion, hyaline-like cartilage would be stimulated to form. (wikipedia.org)
  • Our results support the hypothesis that articular cartilage GAGs are antiadhesive to endothelial cells and suggest that chondroitin sulfate and/or hyaluronan are responsible. (cardiff.ac.uk)
  • This hypothesis was tested by impacting bovine cartilage explants with nominal stresses ranging from 0.5 to 65 MPa and measuring proteoglycan biosynthesis, cell viability, and water content immediately after impaction and 24 hours later. (lievers.net)
Transplantation8
  • another is a method of treating adipose tissue with an enzyme to separate a stromal vascular cell group from other fat cells, which is then used for transplantation, called stromal vascular fraction (SVF). (biomedcentral.com)
  • PRP and cartilage cell transplantation for osteoarthritis are still considered controversial treatments. (lu.se)
  • PRP and cartilage cell transplantation as treatments for osteoarthritis (OA) remain controversial. (lu.se)
  • Cartilage cell transplantation is not a very common procedure and is mainly offered to young and middle-aged individuals with loss of cartilage after suffering a traumatic joint injury. (lu.se)
  • There are various ways of performing cartilage cell transplantation, but the principle is the same for all procedures: to stimulate the growth of new cartilage and repair damaged cartilage. (lu.se)
  • However, there is not enough scientific evidence that cartilage cell transplantation actually works and there is a lack of data comparing the treatment method to placebo. (lu.se)
  • In May 2010, the Sixty-third World Health Assembly adopted resolution WHA63.22,1 in which it endorsed the updated WHO Guiding Principles on Human Cell, Tissue and Organ Transplantation and provided strategic directions to support progress in human organ, tissue and cell donation with the aim of maximizing the benefits of transplantation, meeting the needs of recipients, protecting donors and ensuring the dignity of all involved. (who.int)
  • Outcomes of corneal transplantation in Europe: report by the European Cornea and Cell Transplantation Registry. (who.int)
Hyaline-like cartilage2
Stimulate4
  • Our findings raise the possibility that drugs or other therapies can be developed to stimulate the production of OCR stem cells and improve the body's ability to repair bone injury--a process that declines significantly in old age," says Timothy C. Wang, MD, the Dorothy L. and Daniel H. Silberberg Professor of Medicine at CUMC, who initiated this research. (sciencedaily.com)
  • One way to stimulate the growth of new cartilage is through microfracture . (healthline.com)
  • Experimental studies have indicated that cyclic compressive loads stimulate the biosynthetic activity of cells differently than static compressive loads. (cdc.gov)
  • The treatment aims to release growth factors to stimulate stem cells and produce new cartilage cells. (lu.se)
Differentiation4
  • New perspectives in chondrogenic differentiation of stem cells for cartilage repair. (nus.edu.sg)
  • This overexpression greatly increased the production of cartilage extracellular matrix protein type II collagen and glycosaminoglycans during chondrogenic differentiation. (wustl.edu)
  • IMPACT STATEMENT: Catabolic factors present in a damaged joint inhibit chondrogenic differentiation of mesenchymal stem cells, thereby reducing the chance for successful cartilage formation. (ru.nl)
  • Here we document the effects of specified PEMF parameters over mesenchymal stem cells (MSC) chondrogenic differentiation. (nature.com)
Involving cartilage development1
  • We further characterized the response of these cells to mechanical and inflammatory stimuli such that they could be used for in vitro experiments involving cartilage development, homeostasis and inflammatory disease. (wustl.edu)
Explants5
  • This was investigated using an in vitro angiogenesis model assessing endothelial cell adhesion to GAG-depleted cartilage explants. (cardiff.ac.uk)
  • Bovine cartilage explants were treated with hyaluronidase to deplete GAG content and then seeded with fluorescently tagged human endothelial cells (HMEC-1). (cardiff.ac.uk)
  • Biosynthetic response of cartilage explants to dynamic compression. (lievers.net)
  • Parkkinen JJ, Lammi MJ, Helminen HJ, Tammi M. Local stimulation of proteoglycan synthesis in articular cartilage explants by dynamic compression in vitro. (lievers.net)
  • Burton-Wurster N, Vernier-Singer M, Farquhar T, Lust G. Effect of compressive loading and unloading on the synthesis of total protein, proteoglycan, and fibronectin by canine cartilage explants. (lievers.net)
Extracellular4
Injuries and cartilage2
  • Being able to control the cell fate in this way makes it possible to direct a cell to become bone, cartilage, or fat, which has tremendous implications for creating new treatments for the 1 in 4 people living with cartilage injuries and cartilage degeneration. (drkayvanhaddadan.com)
  • Computed tomography arthrography: Specifically for cartilage injuries and cartilage tears, this image test provides accurate three-dimensional reconstructions of cartilage. (uhhospitals.org)
Tissue engineered cartilage4
  • For the purposes of understanding inflammatory joint disease and the stability of tissue engineered cartilage for clinical repair, we were interested in the regulation of the protein lubricin, and its gene PRG4. (wustl.edu)
  • By using MSC-derived tissue engineered cartilage as a model, we determined that when challenged with IL-1β, PRG4 expression increases, but lubricin content is unchanged. (wustl.edu)
  • These intriguing responses help us better understand progression of inflammatory joint disease as well as informing us as to the potential therapeutic effect, or lack thereof, of lubricin in tissue engineered cartilage when implanted into an inflammatory environment. (wustl.edu)
  • Davisson T, Kunig S, Chen A, Sah R, Ratcliffe A. Static and dynamic compression modulate matrix metabolism in tissue engineered cartilage. (lievers.net)
Clinical7
  • Clinical Relevance: The harvest technique of BM may be of major importance in determining the clinical success of BM mesenchymal stem/stromal cells in cartilage repair. (whiterose.ac.uk)
  • Moreover, in order to extend the potential use of this technique to older people or those with degenerative cartilage pathologies like osteoarthritis, a lot more fundamental and pre-clinical research work needs to be done. (thenakedscientists.com)
  • If you or someone you know has been diagnosed with damaged cartilage or other tissue due to injury or a chronic condition, contact Dr. Bennett's office to learn how his clinical applications of research can help. (bennettorthosportsmed.com)
  • Chondroinduction is the main cartilage repair response to microfracture and microfracture with BST-CarGel: results as shown by ICRS-II histological scoring and a novel zonal collagen type scoring method of human clinical biopsy specimens. (howhelp.org)
  • You can find a list of clinical trials from around the world looking at using stem cells to treat osteoporosis and a whole slew of other diseases here . (supergene.com)
  • The demonstrated efficacy of our optimized PEMF regimens has clear clinical implications for future regenerative strategies for cartilage. (nature.com)
  • It stimulates the cells in the cartilage to make more cartilage," says Marc Hochberg, MD, primary investigator of the study and head of rheumatology and clinical immunology at the University of Maryland School of Medicine. (medscape.com)
Therapies1
  • These results with agarose and pellet culture studies set the stage for the development of conditions appropriate for culturing 3D functional designed cartilage for eventual use in human therapies. (biongenex.com)
Joints11
Progenitor cells1
  • this released a flood of progenitor cells, or cells which eventually create new cartilage. (forbes.com)
Osteoarthritic cartilage2
  • We hypothesize that the low GAG content of osteoarthritic cartilage renders the tissue susceptible to pathological vascularization. (cardiff.ac.uk)
  • Characterisation of a divergent progenitor cell sub-populations in human osteoarthritic cartilage: the role of telomere erosion and replicative senescence by: Christopher Fellows, et al. (swan.ac.uk)
Loss of proteoglycan2
  • Articular cartilage undergoes severe loss of proteoglycan and its constituent glycosaminoglycans (GAGs) in osteoarthritis. (cardiff.ac.uk)
  • Significant evidence exists that trauma to a joint produced by a single impact load below that which causes subchondral bone fracture can result in permanent damage to the cartilage matrix, including surface fissures, loss of proteoglycan, and cell death. (lievers.net)
Produce new cartilage1
Functional3
  • Researchers at Duke University Medical Center have created a three dimensional woven scaffold that could be impregnated with a recipients own stem cells to create functional cartilage. (neatorama.com)
  • In addition, to address the main challenges for the production of functional cartilage: mechanical and lubricant properties. (bvsalud.org)
  • As in many of the lysosomal storage diseases, the functional deficiency of lysosomal enzymes results in abnormal cell architecture. (medscape.com)
Collagen7
  • At 14 and 21 days post-amputation, Dil-labeled cells remained in the subapical space and colocalized with Collagen type II (Col2) staining in the cartilage tube and myosin heavy chain (MHC) staining in regenerated muscle. (pitt.edu)
  • However, there appeared to be a critical threshold stress (15-20 MPa) that caused cell death and apparent rupture of the collagen fiber matrix at the time of impaction. (lievers.net)
  • We concluded that the cell death and collagen rupture are responsible for the observed alterations in the tissue's metabolism and water content, respectively, although the exact mechanism causing this damage could not be determined. (lievers.net)
  • Maroudas A. Balance between swelling pressure and collagen tension in normal and degenerate cartilage. (lievers.net)
  • The biomechanical function of the collagen fibril ultrastructure of articular cartilage. (lievers.net)
  • In contrast, Os-Chon induction resulted in partial mineralization and a large area of cartilage tissue, with greatly increased expression of chondrogenic marker genes along with osterix and collagen 1a1. (elsevierpure.com)
  • Recently, in vitro cartilage studies have shown that impact loading can produce structural damage and osteoarthritis-like changes, including tissue swelling, collagen denaturation, and cell death. (johnshopkins.edu)
Hypoplasia15
  • People with cartilage-hair hypoplasia have unusually short limbs and short stature from birth. (medlineplus.gov)
  • The extent of the immune deficiency in cartilage-hair hypoplasia varies from mild to severe. (medlineplus.gov)
  • Some people with cartilage-hair hypoplasia experience gastrointestinal problems. (medlineplus.gov)
  • Cartilage-hair hypoplasia occurs most often in the Old Order Amish population, where it affects about 1 in 1,300 newborns. (medlineplus.gov)
  • Cartilage-hair hypoplasia is caused by mutations in the RMRP gene. (medlineplus.gov)
  • Cartilage-hair hypoplasia (CHH), which is Online Mendelian Inheritance in Man (OMIM) disease number 250250, is an autosomal recessive inherited disorder that results in short-limb dwarfism associated with T-cell and B-cell immunodeficiency. (medscape.com)
  • [ 1 ] Cartilage-hair hypoplasia and other short-limb dwarfism phenotypes are associated with metaphyseal or spondyloepiphyseal dysplasia. (medscape.com)
  • Cartilage-hair hypoplasia is a variant of short-limb dwarfism in which fine sparse hair is also present. (medscape.com)
  • The immunodeficiency in cartilage-hair hypoplasia may be an isolated T-cell immunodeficiency, isolated B-cell immunodeficiency, or combined T-cell and B-cell immunodeficiency. (medscape.com)
  • Although originally described by McKusik et al in 1964 in Amish children and known as metaphyseal chondrodysplasia McKusick type, cartilage-hair hypoplasia has been described in non-Amish persons throughout the United States, Europe, and Mexico. (medscape.com)
  • [ 2 ] The genetic defect in cartilage-hair hypoplasia has been confirmed to be mutations in the RMRP gene. (medscape.com)
  • The genetic defect in cartilage-hair hypoplasia has been identified as a mutation in the gene for RNAase RMRP, mapped to 9p12. (medscape.com)
  • G, which occurs in 30-50% of patients with cartilage-hair hypoplasia and causes an alteration in ribosomal processing. (medscape.com)
  • This suggests a common cell proliferation defect in cartilage-hair hypoplasia. (medscape.com)
  • Cartilage-hair hypoplasia is a rare defect. (medscape.com)
Type of cartilage2
Response of cartilage1
  • However, only the overall response of cartilage to dynamic loading has been observed to date. (cdc.gov)
Adipose tissue-derived stem cells1
  • At our hospital, our application protocol, titled "Joint treatment by administration of adipose tissue-derived stem cells", has received approval since April 2018. (biomedcentral.com)
Progression2
  • Due to the high incidence of injury and osteoarthritis and the limited nature of articular cartilage self-repair, alternate means are necessary to replace the damaged tissue and regain an articulating surface that not only stops the progression of disease but functions the same as a healthy articular surface. (lievers.net)
  • Their role in cartilage homeostasis and response to inflammation is crucial for understanding the progression and potential therapeutic interventions for various cartilage-related disorders. (lu.se)
Injury10
  • We are now trying to figure out whether we can persuade these cells to specifically regenerate after injury. (sciencedaily.com)
  • These cells are particularly active during development, but they also increase in number in adulthood after bone injury," says Gerard Karsenty, MD, PhD, the Paul A. Marks Professor of Genetics and Development, chair of the Department of Genetics & Development, and a member of the research team. (sciencedaily.com)
  • The proposed treatment may be of major public benefit, as it would represent the first curative strategy for cartilage injury and subsequent degeneration, likely decreasing economic burden on the state and its people. (ca.gov)
  • But more than 2 million people, usually through injury, are diagnosed with cartilage-related joint conditions every year, and the problem is often long-lived. (thenakedscientists.com)
  • In osteoarthritis, usually the cartilage thins in response to aging or joint injury (Figure 1). (forbes.com)
  • In the knees receiving the stem cells, the surface of the injury sites were relatively smooth as in a healthy joint, and cells in the treated area appeared similar to cells in the surrounding normal cartilage. (supergene.com)
  • Board-certified sports medicine doctors at University Hospitals are highly skilled in the diagnostics and treatment of any sports injury, including torn cartilage. (uhhospitals.org)
  • Magnetic resonance imaging ( MRI ) scans: Can show small tears and areas of tendon, ligament, cartilage or other muscle injury. (uhhospitals.org)
  • Release of damaged cells and tissue debris occurs upon injury. (medscape.com)
  • because it doesn't heal well after injury, researchers are not allowed to take samples of cartilage from living patients. (lu.se)
Degeneration3
Damage10
  • The value of autologous osteochondral paste for in-vitro treatment of damage to articular cartilage. (wikipedia.org)
  • Damage to the articular cartilage can be even more problematic than Meniscus tears. (bennettorthosportsmed.com)
  • However, these methods are not able to reverse the damage to cartilage, hence will not 'cure' the underlying cause of osteoarthritis. (supergene.com)
  • The large share and high growth of this segment can be attributed to the high prevalence of cartilage damage in hyaline cartilage. (radiantinsights.com)
  • Rabbits given the implants, which generate electricity from mechanical forces as the animals move around, experienced more healing after cartilage damage than those given a placebo device. (newscientist.com)
  • Matrix damage and chondrocyte viability following a single impact load on articular-cartilage. (lievers.net)
  • Our experts in minimally invasive surgical interventions can repair the cartilage damage and offer you faster healing, less bleeding, and a speedier recovery. (uhhospitals.org)
  • A number of direct and indirect radiation interaction pathways can produce damage to the DNA of irradiated cells. (cdc.gov)
  • Cartilage is unable to repair itself once the damage is extensive enough to be visible to the naked eye. (lu.se)
  • Damage at molecular level - within the molecules of the cartilage - can be repaired through the ongoing renewal of the components of the cartilage. (lu.se)
Mechanical4
  • Although the mechanical properties were inferior to native articular cartilage, they were within range of repair tissue. (lievers.net)
  • Torzilli PA, Grigiene R, Huang C, Friedman SM, Doty SB, Boskey AL, Lust G. Characterization of cartilage metabolic response to static and dynamic stress using a mechanical explant test system. (lievers.net)
  • Literature review: The articular cartilage is a highly specialized tissue that reduces joint friction and distributes forces related to high mechanical loads between bone ends. (bvsalud.org)
  • Moreover, one should consider the application of mechanical stimuli and surface properties to produce an articular cartilage with satisfactory characteristics for in vivo application. (bvsalud.org)
Avascular tissue2
  • Articular cartilage is an avascular tissue with low potential for self-repair. (nature.com)
  • IntroductionHuman cartilage is an avascular tissue with limited capacity for repair. (pitt.edu)
Microfracture1
  • In terms of procedure type, microfracture and ACI are the most common procedure observed amongst all the types of cartilage repair procedure. (radiantinsights.com)
Normal cartilage1
Bones6
  • They typically have malformations in the cartilage near the ends of the long bones in the arms and legs (metaphyseal chondrodysplasia), which then affects development of the bone itself. (medlineplus.gov)
  • Meniscus cartilage serves as a cushion or shock absorber between the bones. (healthline.com)
  • This is because,, except for some small bones at the bridge, the bulk of the nose is built from nasal cartilage. (thenakedscientists.com)
  • It involves the wear and tear of cartilage , a rubbery layer capping the ends of bones that normally stops them rubbing together. (newscientist.com)
  • The skeleton (which includes bones, ligaments, tendons, and cartilage) and muscles that are attached to it make up the musculoskeletal system . (cdc.gov)
  • Overview of Bone Tumors Bone tumors are growths of abnormal cells in bones. (msdmanuals.com)
Vitro cartilage1
Lost cartilage1