Carrier protein conjugate sulfhydryl carrier proteins. Medical search. Frequent questions

Perform hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens with this sulfhydryl-reactive mariculture KLH. (fishersci.com)
Thermo Scientific™ Imject Maleimide-Activated mcKLH is sulfhydryl-reactive immunogenic mariculture keyhole limpet hemocyanin (KLH) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens. (fishersci.com)
Maleimide groups form covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other thiol-containing haptens. (fishersci.com)
Cysteine -- A sulfur-containing nonessential amino acid produced by the enzymatic or acid hydrolysis of proteins. (nih.gov)
Cysteine engineered anti-MUC16 antibodies (Ab) are conjugated with one or more drug moieties (D) through a linker (L) to form cysteine engineered anti-MUC16 antibody-drug conjugates having Formula I: Ab-(L-D)p I where p is 1 to 4. (justia.com)
The cysteine engineered antibodies may be conjugated with chemotherapeutic drugs, toxins, affinity ligands such as biotin, and detection labels such as fluorophores. (justia.com)
We are also focused on the development of chemoselective, convenient and mild synthetic strategy to modify the cysteine sulfhydryl group of peptide sequences in order to introduce different functionalities, such as carbohydrate moieties. (cnr.it)

Examples of biological sources are serum proteins, lectins and antibodies. (wikipedia.org)
The invention also relates to methods of using antibodies and antibody-drug conjugate compounds for in vitro, in situ, and in vivo diagnosis or treatment of mammalian cells, or associated pathological conditions. (justia.com)
Thorpe, (1985) "Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A Review," in Monoclonal Antibodies '84: Biological And Clinical Applications, A. Pinchera et al (ed.s), pp. 475-506). (justia.com)
Some cytotoxic drugs tend to be inactive or less active when conjugated to large antibodies or protein receptor ligands. (justia.com)
Among them AAT Bioquest's Buccutite™ crosslinking technology offer the most efficient method to conjugate antibodies with macromolecules such as enzyme tags. (stratech.co.uk)
The long spacer arm reduces steric hindrance and ensures greater binding of proteins and antibodies during affinity purification. (gbiosciences.com)
G-Biosciences Immobilized Monomeric Avidin Resin is designed for the simple affinity chromatography purifications of proteins, antibodies and other molecules with a biotin tag. (gbiosciences.com)
Biotinylated liposomes have a biotin moiety and are primarily used for conjugation to proteins with (streptavidin) affinity or biotin, such as antibodies. (bocsci.com)
BOC Sciences manufactures biotinylated liposomes that have been widely used in preclinical and laboratory applications, and have demonstrated good efficiency and success in conjugating antibodies especially downstream, assisting customers in advancing the development of immunotherapies. (bocsci.com)

Chymotrypsin -- A protease that catalyses the hydrolysis (the breakdown) of proteins into peptides or amino acids in the small intestine. (nih.gov)
Alignment of the amino acid sequence of aprotinin with the Kunitz domains of human proteins allowed the identification and design of a family of peptides, named Angiopeps. (aspetjournals.org)
The Sulfhydryl Immobilization Kit for Peptides is designed for the simple and efficient coupling of sulfhydryl-containing peptides to a solid agarose support. (gbiosciences.com)
Peptides must have free sulfhydryls for immobilization to the resin. (gbiosciences.com)
It is a linear PEG derivative with 18 linear carbon straight chains and a different reactive group at the other end that can modify peptides, proteins, lipids or small molecules. (bocsci.com)

containing a basic amino group, an acidic carboxyl group, and a side chain attached to an alpha carbon atom, and that link together by peptide bonds to form proteins, or that function as chemical messengers and as intermediates in metabolism. (nih.gov)
Here, we synthesize an electrically charged cationic formulation of IGF-1 by using a short-length arginine-rich, hydrophilic cationic peptide carrier (CPC) with a net charge of +14, designed for rapid and high uptake and retention in both healthy and arthritic cartilage. (biomedcentral.com)
Protein-S-S-Reductant™ offers the advantage that it does not require removal before peptide immobilization. (gbiosciences.com)
Sepahi, Mina et al (2021) Optimization of the Endotoxin Removal Performance of Solid-Phase Conjugated S3E3 Antimicrobial Peptide Using Response Surface Methodology. (gbiosciences.com)
Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)
at the same time developing protein and peptide-based tools that will allow us to manipulate cellular processes on a molecular level. (stanford.edu)
Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the gamma-secretase complex which cleave the amyloid precursor protein (APP) at what are known as the gamma- and epsilon-sites and can lead to the accumulation of the Amyloid beta peptide (Abeta) cleavage product that is associated with Alzheimer's disease. (agtcbioproducts.com)
Here, we report a TMPRSS2-responsive surface-potential-tunable peptide-conjugated probe (EGTP) with aggregation-induced emission (AIE) features for TMPRSS2 selective imaging and accurate inhibitor screening. (bvsalud.org)

For immunoprecipitation experiments, HA-tag or Myc-tag antibody-conjugated agarose beads had been purchased from Cellular Signaling Technology. (healthweblognews.info)

Select from among several package sizes of activated KLH protein, or choose the complete conjugation kit that includes optimized buffers and trouble-free spin desalting columns. (fishersci.com)

Affinity chromatography can be used in a number of applications, including nucleic acid purification, protein purification from cell free extracts, and purification from blood. (wikipedia.org)
By using affinity chromatography, one can separate proteins that bind to a certain fragment from proteins that do not bind that specific fragment. (wikipedia.org)
Affinity chromatography refers to the use in chromatography of particular materials as adsorbents of groups which are supposed to be specific ligands of the protein being purified. (nih.gov)

Numerous antigen molecules can be conjugated to each KLH molecule to make them highly immunogenic and increase the likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest. (fishersci.com)

The high-quality mariculture KLH is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer. (fishersci.com)
In the interaction with proteins, lead binds with virtually every available functional group, including sulfhydryl, amine, phosphate, and carboxyl groups, with sulfhydryl having the highest affinity. (cdc.gov)

Glutathione is the most abundant intracellular thiol (i.e., a compound containing a sulfhydryl [SH] group) and low molecular weight tripeptide found in living cells. (betterhealthinternational.org)

An assortment of reagents for the purification of biotin labelled proteins are available from G-Biosciences. (gbiosciences.com)
Accurate estimation of the molar ratio of biotin conjugated to protein/antibody is easy and affordable with HOOK™ BiotinQuant™ Kit . (gbiosciences.com)
HOOK™ Cell Surface Protein Isolation Kit uses G-Biosciences HOOK™ biotin labeling and purification technology in conjunction with our Mammalian Cell PE LB™ lysis buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting. (gbiosciences.com)
HOOK™ BiotinQuant™ uses HABA (2-(4-Hydroxyphenylazo)benzoic acid/ 2-(4′-Hydroxybenzeneazo)benzoic acid/ 4′-Hydroxyazobenzene-2-carboxylic acid) to rapidly estimate the mole-to-mole ratio of biotin to antibody or protein. (gbiosciences.com)
Biotin belongs to the B vitamins, also known as Vitamin H, Vitamin B7 and Coenzyme R. Biotin is a coenzyme for a variety of carboxylases and acts as a CO 2 carrier in carboxylase reactions. (bocsci.com)
In particular, biotin binds strongly and specifically to affinin or streptavidin proteins and can be used in a variety of biological and biochemical applications such as protein purification, cell labeling, and for the preparation of biotinylated molecules. (bocsci.com)
Anti-biotin proteins and streptavidin anti-biotin proteins have multiple biotin binding sites and the interaction between biotin and these proteins is one of the strongest non-covalent interactions in biology. (bocsci.com)
By first attaching biotin to the surface of the liposome and then attaching a drug or therapeutic agent that is conjugated to an anti-biotin protein or streptavidin anti-biotin protein, the liposome can be targeted to specific cells or tissues that express anti-biotin protein/streptavidin anti-biotin protein receptors. (bocsci.com)

There are a variety of methods available for chemically conjugating two different target molecules. (imgenbiosciences.com)
Although lipids are a distinct class of biomolecules, we shall see that they often occur combined, either covalently or through weak bonds, with members of other classes of bio-molecules to yield hybrid molecules such as glycolipids , which contain both carbohydrate and lipid groups, "and lipoproteins , which contain both lipids and proteins. (medmuv.com)
Antibody -- Any of the protein molecules produced by specialized immune system cells (B cells) that can recognize and bind to a particular foreign antigen. (nih.gov)
Drug moieties used in antibody drug conjugates include bacterial protein toxins such as diphtheria toxin, plant protein toxins such as ricin, small molecules such as auristatins, geldanamycin (Mandler et al (2000) J. of the Nat. (justia.com)

antigen and antibody, enzyme and substrate, receptor and ligand, or protein and nucleic acid binding interactions are frequently exploited for isolation of various biomolecules. (wikipedia.org)
iFluor™ superior fluorescent labelling dyes, optimised for labelling proteins and nucleic acids. (stratech.co.uk)
Frequently used methods are based on, for example, antigen-antibody systems, nucleic acid hybridization techniques, and protein-ligand systems. (justia.com)

Because this technique of purification relies on the biological properties of the protein needed, it is a useful technique and proteins can be purified many folds in one step. (wikipedia.org)

Disulfide interchange enzyme -- An enzyme that catalyzes the rearrangement of disulfide bonds within proteins during folding. (nih.gov)
The supplied Protein-S-S-Reductant™ reducing agent efficiently reduces disulfide bonds and does not interfere with the iodoacetyl coupling reaction. (gbiosciences.com)

POLK and POLZ have in the receptor of proteins reused multiple to such anions by POLK. (erik-mill.de)
Nuclear Receptors the nuclear receptor family consists of two types of receptors that have similar protein structure. (spiritsong.org)

IGF-1 was conjugated to CPC by using a site-specific sulfhydryl reaction via a bifunctional linker. (biomedcentral.com)

Use Intercept Protein-Free Blocking Buffer in labs where animal-based product use is restricted. (licor.com)
This diluent contains Intercept (PBS) Protein-Free Blocking Buffer preformulated with Tween 20. (licor.com)

In its binding with sulfhydryl groups, lead may interfere with the activity of zinc metalloenzymes, as zinc binds to a sulfhydryl group at the active site. (cdc.gov)
Lead also binds to metallothionein, a sulfhydryl-rich protein, but does not appear to displace cadmium or zinc. (cdc.gov)

Herein, carbodiimide hydrochloride/N-hydroxysuccinimide was used to mediate the grafting of gallic acid (GA) (0.005, 0.0015, and 0.025 wt%) with soybean protein isolate (SPI) in the preparation of SPI-GA conjugates and hydrogels. (bvsalud.org)
Dextran dialdehyde (ODex) was added to a nanocomplex of soy protein isolate (SPI)-curcumin (Cur) to improve its stability and achieve controlled release of Cur. (bvsalud.org)
Herein, we used pH-shifted and pH-driven methods to assemble kidney-bean protein isolate (KPI) and luteolin (Lut) into a nanocomplex and subsequently investigated their binding mechanism, structure, and functional properties. (bvsalud.org)

In affected indivi- chemicals possessing the ability to react with duals, it has a serious impact on their quality of proteins, and hence these methods do not esti- life. (cdc.gov)
The basis of hapten- molecular mechanisms of the sensitization pro- protein binding work is the hypothesis that upon cess will result in novel opportunities for the skin absorption, only protein-reactive chemicals development of alternative methods for assessing (or those that can be metabolically or chemically skin sensitization hazard and relative potency of converted to protein-reactive species) are able to chemicals. (cdc.gov)
Materials and Methods Cell lines, plasmids, recombinant proteins All cells were maintained in DMEM (4.5?g/L glucose) supplemented with 10% FBS and Penicillin/Streptomycin (Gibco). (healthweblognews.info)
For biomedical applications, we are combining rational design and combinatorial methods to create designer protein therapeutics and diagnostic agents. (stanford.edu)

Twenty alpha-amino acids are the subunits which are polymerized to form proteins. (lookformedical.com)
It is a 153 amino acids protein that in humans is encoded by the EIF5A2 gene. (operatiebrp.nl)

Screen Quest™ assay kits, a set of HTS-ready tools for high throughput screening of biochemical and cellular targets such as protein kinases, proteases, HDAC, cell apoptosis and cytoxicity, GPCR, ion channels, ADME/metabolism and transporters. (stratech.co.uk)

The Sulfhydryl Coupling Resin Columns utilizes iodoacetyl groups that specifically react with free sulfhydryls to form covalent, permanent thioether bonds (see figure). (gbiosciences.com)

It causes the production of abnormal proteins with impaired biological activity. (lookformedical.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Wilms Tumor Protein (WT1) in tissue homogenates, cell lysates and other biological fluids. (kits-elisa.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Wilms Tumor Protein (WT1) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (kits-elisa.com)
Description: A sandwich ELISA kit for detection of Wilms Tumor Protein from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (kits-elisa.com)
Description: Quantitative sandwich ELISA for measuring Human Wilms tumor protein (WT1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. (kits-elisa.com)

One of the key molecular events in skin sensitization is protein haptenation, i.e. the chemical modification of self-skin protein(s) thus forming macromolecular immunogens. (cdc.gov)

Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE. (agtcbioproducts.com)

Colchicine may impair vitamin A absorption and can interfere with retinol homeostasis by blocking release of retinol--retinol binding protein (RBP). (interactionsguide.com)

Transmembrane protease serine 2 (TMPRSS2) is a plasma membrane protease that activates both spike protein of coronaviruses for cell entry and oncogenic signaling pathways for tumor progression. (bvsalud.org)

To be somatic nucleus nucleotide through coupled uracil kDa at such cancers, transporters serve a scaffold, replaced to as activation deficit( TLS), which translocates form protein to Get complex classes. (erik-mill.de)

The use of antibody-drug conjugates (ADC), i.e. immunoconjugates, for the local delivery of cytotoxic or cytostatic agents, i.e. drugs to kill or inhibit tumor cells in the treatment of cancer (Lambert, J. (2005) Curr. (justia.com)
The enterohepatic cycling of some drugs is dependent on intestinal bacteria that hydrolyze drug conjugates excreted by the bile and thereby enable the more lipid-soluble parent compound to be reabsorbed into the circulation. (spiritsong.org)
These techniques are particularly suitable for developing environmentally sustainable and energy-efficient synthetic process to prepare biopolymer-based nano-sized material to be employed as drug delivery carrier systems. (cnr.it)
Dr. Cochran's translational interests span protein-based drug discovery and development for applications in oncology and regenerative medicine, and the development of new technologies for high-throughput protein analysis and engineering. (stanford.edu)
2. Huang L, Song J, Chen B. A novel targeting drug carrier to deliver chemical bonded and physical entrapped anti-tumor drugs. (bocsci.com)

Revert 520 Total Protein Stain is used to stain all sample protein in Western blot lanes so that the total amount of protein in each lane can be used as a basis for accurate and reliable normalization. (licor.com)

Clear indices of cell death-myocyte disruption, coagulative necrosis, elevation of cardiac proteins in serum-are present by 24 hours. (spiritsong.org)

a sensitive and robust tool for detection of proteins in solutions and solid surfaces (such as gels and membranes). (stratech.co.uk)
Black blot incubation boxes are great for incubating near-infrared fluorescent Western blots, chemiluminescent Western blots, and protein gels. (licor.com)

These include our costumers' specific requirements for the end products, the availability of functional groups in the targets (e.g., amines, carboxyls and sulfhydryls), and the lengh and type of linkers preferred. (imgenbiosciences.com)

In conclusion, these capabilities provide numerous possibilities for the development of multifunctional and active substance delivery carriers. (bvsalud.org)

These conjugates find use in single molecule DNA sequencing and other applications. (justia.com)

By controlling access to the brain, the blood-brain barrier (BBB) restricts the entry of proteins and potential drugs to cerebral tissues. (aspetjournals.org)
Exogenous steroid administration may cause transient increases in levels vitamin A and retinol-binding protein (RBP), whereas extended use may reduce levels of vitamin A and metabolites in plasma and tissues. (interactionsguide.com)

The sialic examples of L1CAM proteins have most covalently predicted. (erik-mill.de)

It is always present during the acute phase of the infection and in the chronic carrier phase. (spiritsong.org)
Long-term activation of protein kinase c causes chronic Na/H antiporter stimulation in cultured proximal tubule cells. (jci.org)
To examine the role of protein kinase C as a chronic regulator of proximal tubule Na/H antiporter activity, the effect of phorbol 12-myristate 13-acetate (PMA) on the Na/H antiporter was studied in cultured proximal tubule cells. (jci.org)

Heme proteins like hemoglobin (Hb) and myoglobin (Mb) contain redox-active transition metal iron that makes them susceptible to causing oxidative damage. (scialert.net)

Intercept T20 Protein-Free Antibody Diluents improve antibody specificity and can be used where animal-based products are prohibited. (licor.com)

Short-term activation of protein kinase C by 5 min exposure to PMA caused an acute increase in Na/H antiporter activity that was not prevented by cycloheximide or actinomycin D and did not persist 24 h later. (jci.org)
Long-term activation of protein kinase C by 2 h exposure to PMA caused a dose-dependent increase in Na/H antiporter activity 24 h later. (jci.org)
This latter effect was due to protein kinase C activation in that it was inhibited by sphingosine and was not seen with 4 alpha-PMA, an inactive analogue. (jci.org)
In conclusion, short-term activation of protein kinase C leads to a transient increase in Na/H antiporter activity that is independent of transcription and translation, whereas long-term activation of protein kinase C causes a persistent increase in antiporter activity that is dependent on transcription and translation and is associated with increased mRNANa/H abundance. (jci.org)

However, it is unclear how the Schulze-Hardy rule works when the simple electrolytes are replaced with more sophisticated charge carriers. (bvsalud.org)

Anatomy8

Organisms20

Diseases1

Chemicals and Drugs144

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes51

Disciplines and Occupations1

Technology, Industry, Agriculture3

Information Science4

Health Care4

Cysteine7

Antibodies9

Peptides5

Peptide8

Antibody Conjugated1

Conjugation1

Affinity chromatography3

Highly immunogenic1

Phosphate2

Thiol1

Biotin8

Molecules4

Nucleic3

Purification1

Disulfide bonds2

Receptor2

Linker1

Buffer2

Binds2

Isolate3

Methods4

Amino acids2

Metabolism1

Resin1

Biological5

Immunogens1

Molecular weight1

Interfere1

Protease1

Form1

Drug5

Stain1

Cell1

Gels2

Groups1

Numerous1

Applications1

Tissues2

Examples1

Chronic3

Active1

Free1

Activation4

Simple1