• We established a novel technique for quantifying [Na + ] i in cultured neonatal rat cardiomyocytes attached to a 96-well plate using a microplate reader in combination with SBFI and probenecid. (biomedcentral.com)
  • NSE and OEA act as modulators of electrical excitability of the plasma membrane of neonatal rat cardiomyocytes by affecting the action potential duration. (org.ua)
  • In one model, we administered clinical doses of the leukemia therapeutic drug imatinib to neonatal ventricular cardiomyocytes. (temple.edu)
  • In ESD3, 6-gingerol severely affected differentiation into cardiomyocytes cell activity and protein content at both low and high concentrations. (nottingham.ac.uk)
  • these are neurons that have reached the extreme stage of differentiation, cardiomyocytes, granular leukocytes and others. (vechnayamolodost.ru)
  • In particular, the cardiac movement of HL-1 was significantly reduced after PM 2.5 treatment. (nih.gov)
  • This is because cardiac remodeling occurs. (com.pk)
  • As a demonstration of the versatility of the Swarm, we also developed an assay measuring cardiac action potential and calcium waveform properties simultaneously under paced conditions using human induced pluripotent stem (iPS) cell-derived cardiomyocytes as an additional counter screen for cardiac toxicity. (frontiersin.org)
  • The researchers discovered that reducing an outer mitochondrial membrane protein, FUN14 domain containing 1 (FUNDC1), in cardiac muscle cells , also known as cardiomyocytes, activates and worsens cardiac dysfunction . (medicalxpress.com)
  • The researchers used mouse neonatal cardiomyocytes, mice with a genetic deletion of the FUNDC1 gene, control mice with no genetic deficiencies and the cardiac tissues of patients with heart failure. (medicalxpress.com)
  • Splenectomy decreased the numbers of circulating inflammatory Ly6C hi monocytes in blood, reduced the numbers of proinflammatory cardiac macrophages and significantly improved the post-MI LV function in Hmox1 −/− mice. (springer.com)
  • The short-term beat-to-beat variability of cardiac action potential duration (SBVR) occurs as a random alteration of the ventricular repolarization duration. (unideb.hu)
  • Any loss of electrical coordination between cardiomyocytes can lead to irregular heartbeats, or worse sudden cardiac death. (vt.edu)
  • The shortening of telomeres in cardiomyocytes appears to be a reliable hallmark of cardiac failures that arise due to genetic defects, and it's very specific to cells that require the missing contractile proteins such as dystrophin, troponin T or myosin heavy chain, among others," said Helen Blau, PhD, professor of microbiology and immunology and member of the Stanford Cardiovascular Institute. (defytime-emea.com)
  • Res effectively suppress the cardiomyocytes hypertrophy and apoptosis induced by ISO, characterized by the reduction of the myocardial cell surface area, the ANP gene expression, the LDH and MDA leakage amount and the rate of cell apoptosis, while decrease of the protein expression of GRP78, GRP94 and CHOP, and reverse the expression of Bcl-2 and Bax. (karger.com)
  • Myocardial hypertrophy occurs in many cardiovascular diseases. (researchsquare.com)
  • To test this, we have used a mouse model possessing an AMPK γ2 mutation (R299Q knock-in) and measured demembranated trabeculae mechanics and cardiomyocyte contractility using heterozygous and homozygous male mice, along with littermate wild type controls, between 6-8 weeks of age (prior to hypertrophy and significant glycogen accumulation). (ox.ac.uk)
  • The reduction in PKA activity in the mutant hearts may be caused by novel crosstalk between the AMPK and PKA pathways, in particular as this occurs prior to hypertrophy. (ox.ac.uk)
  • however, here, we focus on the mechanisms of cardiomyocyte dysfunction and treatments of SIC and hope to provide further insights for the management of SIC . (frontiersin.org)
  • Chronic overexpression of MT or acute in vitro treatment with the flavoprotein inhibitor diphenyleneiodonium or the angiotensin II type I receptor antagonist losartan eliminated excess ROS production in diabetic cardiomyocytes. (diabetesjournals.org)
  • Measurement and analysis of sarcomere length in rat cardiomyocytes in situ and in vitro. (ox.ac.uk)
  • It has been demonstrated that the immobilization of NAE in new oligomeric carriers retains completely the cytoprotective activity of N-acylethanolamines in vitro , but at the same time improves significantly (50%) the antitumor activity and speeds up the delivery of substances (including doxorubicin) immobilized in these carriers to target cells. (org.ua)
  • The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)
  • In conclusion, the present study demonstrated that SA inhibits the apoptosis of H9c2 cardiomyocytes following H/R injury via reduced activation of the p38MAPK and JNK signaling pathways. (spandidos-publications.com)
  • The results demonstrated that SA inhibited apoptosis signaling in H9c2 cardiomyocytes via downregulation of p38 mitogen-activated protein kinase (p38MAPK) and c-Jun N-terminal kinase (JNK) signaling pathways following hypoxia/reoxygenation (H/R) injury. (spandidos-publications.com)
  • Scientists hypothesized that the presentation of PEG-MGF resulted in significantly reduced cardiomyocyte damage in mice. (com.pk)
  • Significantly lower incidence of left ventricle (LV) free wall rupture was noted between 3rd and 5th day after MI in Hmox1 −/− mice resulting in their better overall survival. (springer.com)
  • Myocyte-restricted Hmox1 transgenic mice exposed to MI exhibited significantly improved survival and LV function, lower interstitial fibrosis and oxidative stress. (springer.com)
  • Cardiomyocytes from mutant mice exhibited a greater response to isoproterenol. (ox.ac.uk)
  • Calcium activity was enhanced at the cardiomyocyte level in both mouse lines of exercised mice. (temple.edu)
  • In silico prediction analysis with IPA predicted that upregulation of mitochondria depolarization, ROS generation, cardiomyopathy, suppression of Ca 2+ homeostasis, mitochondrial function, and ATP synthesis occurred in PM 2.5 -treated HL-1 cells. (nih.gov)
  • Also, the contact between ER and mitochondria in failed hearts was significantly reduced. (medicalxpress.com)
  • Eugenol (holy basil) at moderate to high concentrations, significantly decreased contractility, cell activity and protein content. (nottingham.ac.uk)
  • To address these hypotheses, we examined contractility, Ca 2+ handling, and ROS levels in individual cardiomyocytes isolated from control hearts, diabetic OVE26 hearts, and diabetic hearts overexpressing antioxidant protein metallothionein (MT). Our data showed that diabetic myocytes exhibited significantly reduced peak shortening, prolonged duration of shortening/relengthening, and decreased maximal velocities of shortening/relengthening as well as slowed intracellular Ca 2+ decay compared with control myocytes. (diabetesjournals.org)
  • This is especially critical when working with rare or hard-to-transfect cells such as iPSCs, neurons or cardiomyocytes, making genome editing tedious. (cytosurge.com)
  • Many types of cardiovascular diseases (CVDs) are associated with complex immune responses that can significantly contribute to their progression and remission. (biomedcentral.com)
  • To illustrate the utility of the new method, we also examined the chronic effects of aldosterone on [Na + ] i in cultured cardiomyocytes. (biomedcentral.com)
  • Our study found the formation of MAMs mediated by the mitochondrial membrane protein FUNDC1 was significantly suppressed in patients with heart failure, which provides evidence that FUNDC1 and MAMs actively participate in the development of heart failure," said Dr. Ming-Hui Zou, director of the Center for Molecular and Translational Medicine at Georgia State and a Georgia Research Alliance Eminent Scholar in Molecular Medicine. (medicalxpress.com)
  • Upon myocardial infarction (MI) immune system becomes activated by extensive necrosis of cardiomyocytes and release of damage-associated molecular patterns [ 16 ]. (springer.com)
  • Mechano growth factor (MGF) is a naturally occurring compound that has been isolated in muscle, bone, heart, and nerve tissue. (com.pk)
  • Syringic acid (SA), a naturally occur-ring O‑methy-lated trihydroxybenzoic acid monomer extracted from Dendrobium nobile Lindl. (spandidos-publications.com)
  • Excess iron in the form of non-transferrin-bound iron (NTBI) causes injury and is taken up by cardiomyocytes, pancreatic islet cells, and hepatocytes. (medscape.com)
  • The mode of the distribution of SL measurements in resting Langendorff-perfused heart is 1.95 mum (n = 167 measurements from N = 11 hearts) after correction for tissue orientation, which was significantly greater than that in isolated cells (1.71 mum, n = 346, N = 9 isolations) or ventricular slice preparations (1.79 mum, n = 79, N = 3 hearts) under our experimental conditions. (ox.ac.uk)
  • Our results highlight that the proposed method can be used to monitor SL in living cells and that different experimental models from the same species may display significantly different SL values under otherwise comparable conditions, which has implications for experiment design, as well as comparison and interpretation of data. (ox.ac.uk)
  • However, surgical detorsion-induced reperfusion injury may occur, and germ cells cannot be regenerated by the surgery [ 1 ]. (biomedcentral.com)
  • Coupling of single cardiomyocytes significantly decreases the beat-to-beat changes in action potential duration (APD) due to the electrotonic current flow between neighboring cells. (unideb.hu)
  • Cell renewal can occur quite intensively: for example, connective tissue cells in the pancreas are replaced every 24 hours, gastric mucosa cells - every three days, leukocytes - every 10 days, skin cells - every six weeks, approximately 70 g of proliferating small intestine cells are removed from the body daily [1]. (vechnayamolodost.ru)
  • Tissue regeneration occurs due to the proliferation of stem cells, which can not only divide, but also differentiate into cells of the tissue whose regeneration is taking place. (vechnayamolodost.ru)
  • During cell division, tissue rejuvenation occurs: new cells take the place of damaged ones, while repair (elimination of DNA damage) occurs more intensively and regeneration is possible in case of tissue damage. (vechnayamolodost.ru)
  • It is not surprising that vertebrates have significantly higher life expectancy than invertebrates - the same insects whose cells do not divide in adulthood. (vechnayamolodost.ru)
  • In addition, extracellular, administered Tβ 4 promoted the cell migration of various types of cells including cardiomyocytes, human umbilical vein endothelial cells and conjunctival epithelial cells ( 4 - 6 ). (spandidos-publications.com)
  • The heart is made of cells called cardiomyocytes that allow for electrical signals to flow through the cells. (vt.edu)
  • The finding dovetails with a previous study showing that people with Duchenne muscular dystrophy, a genetic muscle-wasting disease, also have short telomeres in their heart muscle cells, or cardiomyocytes. (defytime-emea.com)
  • In contrast, the telomere length in nonbeating heart cells of the blood vessels did not vary significantly between the two groups. (defytime-emea.com)
  • Chang saw similar results in cardiomyocytes generated from induced pluripotent stem cells: Those generated from people with cardiomyopathies had significantly shorter telomeres than those generated from unaffected relatives. (defytime-emea.com)
  • In recent years, extensive research has established that some immune cells either reside in the heart or have very complicated interactions with cardiomyocytes through permanent blood circulation. (biomedcentral.com)
  • Arterial thromboses significantly narrow or completely block arterial blood flow and oxygen delivery to tissues. (wikipedia.org)
  • Circulating Pneumolysin, another common PAMP produced by Streptococcus pneumoniae , induced cardiomyocyte injury through triggering profound calcium influx during pneumococcal infection ( 11 ). (frontiersin.org)
  • The shortening of the action potential duration in response to NSE and OEA occurs as the result of both potassium currents potentiation and the inhibition of incoming calcium current through calcium channels of L-type. (org.ua)
  • Through this mechanism, TMZ is thought to correct disturbances in myocardial cellular homeostasis that result from acute ischemic damage and to protect against cardiomyocyte injury [ 14 , 15 ]. (hindawi.com)
  • Although both adapted viruses evoked diffuse ditional signs typical of either secondary bacterial pneumo- alveolar damage and showed a similar 50% mouse lethal dose and the same peak lung concentration, each had a nia or acute respiratory distress syndrome (ARDS) occur. (cdc.gov)
  • This study assessed the deleterious effects of diesel-derived PM 2.5 exposure in HL-1 mouse cardiomyocyte cell lines. (nih.gov)
  • We show that PIONEER-predicted oncoPPIs are significantly associated with patient survival and drug responses from both cancer cell lines and patient-derived xenograft mouse models. (bvsalud.org)
  • H9c2 rat cardiomyocyte cell line was obtained from the American Type Culture Collection (Manassas, VA, USA. (spandidos-publications.com)
  • Mutations occur rarely, and in order for a cell to become cancerous - this is calculated for human fibroblasts - about 100 divisions must occur (this number of divisions usually occurs in a person at about the age of 40) [5]. (vechnayamolodost.ru)
  • Ferroptosis is a new type of programmed cell death, which occurs with iron dependence. (nature.com)
  • But cardiomyocytes divide infrequently, and their telomere lengths remain relatively stable throughout one's life. (defytime-emea.com)
  • The results demonstrated that this 96-well plate based measurement has merits, especially for screening test of compounds regulating [Na + ] i , and is useful to elucidate the mechanisms and consequences of altered [Na + ] i handling in cardiomyocytes. (biomedcentral.com)
  • It is not clearly defined how this process occurs in the cardiomyocyte, or how the pathology can be effectively treated. (temple.edu)
  • In this regard, TMZ may protect cardiomyocytes against excessive oxygen free radical (OFR) production that occurs in ischemia and inflammation and that initiates oxidation of lipids and proteins and damages DNA. (hindawi.com)
  • Acetaminophen toxicity, which can lead to hepatotoxicity, is a burden on our health care system and contributes significantly to intensive care unit admissions and cost of hospitalization. (neurotransmitter.net)
  • In this respect, progranulin (PGRN) serves as one of the main components of the regulation of inflammatory processes, which significantly contributes to the immunopathogenesis of such disorders. (biomedcentral.com)
  • This cardiomyopathy may be due to either organ-based damage, such as fibrosis, or to direct damage to cardiomyocytes. (diabetesjournals.org)
  • Thus, we provide scientists with a state-of-the-art tool allowing them to significantly enhance gene editing rate, optimize on/off-target effects and even enable easy multiplexing of CRISPR mutations. (cytosurge.com)
  • Chang compared the telomere length in cardiomyocytes from 11 patients with dilated or hypertrophic cardiomyopathy due to genetic mutations with nine people who had died from causes unrelated to heart disease. (defytime-emea.com)
  • Because we found in a previous study that cardiomyocytes from boys who had died of Duchenne muscular dystrophy had telomeres that were about 50 percent shorter than those from individuals without the disease," Blau said, "we wondered whether people with other genetic heart conditions, such as cardiomyopathies, might also have cardiomyocytes with abnormally shortened telomeres. (defytime-emea.com)
  • Rapidly absorbed, but bioavailability significantly reduced with food intake. (medscape.com)
  • In general, approximately 50% of patients with sepsis and septic shock underwent septic cardiomyopathy and once septic cardiomyopathy occurred, the mortality rate of those patients reached from 27% to as high as 70% (3,4). (balkanmedicaljournal.org)
  • Dilated cardiomyopathy occurs when the left ventricle is enlarged, while hypertrophic cardiomyopathy is caused by a thickening of the heart muscle. (defytime-emea.com)
  • Results: Among 12 cytokines, IL-6, IL-8, IL-10, and TNF-alpha were significantly elevated in patients with resistance against lamivudine compared with patients maintaining response. (jakenzyme.com)
  • It is common for stress hyperglycemia to occur followed by AMI for nondiabetic patients. (hindawi.com)
  • To determine if FUNDC1 reduction occurred in human hearts and contributed to heart failure in patients, the researchers examined four heart specimens from heart failure patients and four heart specimens from control donors. (medicalxpress.com)
  • They found the levels of FUNDC1 were significantly reduced in patients with heart failure compared to control donors. (medicalxpress.com)
  • Although it's not yet known whether the stunted telomeres directly affect the function of the cardiomyocytes or arise as a result of heart failure, the finding opens the door to an intriguing line of research and drug discovery. (defytime-emea.com)
  • Heart weight to body weight ratio was significantly higher after exercise. (temple.edu)
  • Патофізіологія Congenital heart disease is the most common congenital anomaly, occurring in almost 1% of live births ( 1). (msdmanuals.com)
  • this occurs as a function of time ex vivo and correlates with tissue volume changes determined by magnetic resonance imaging. (ox.ac.uk)
  • Generally, wound healing in the oral cavity is known to occur more quickly and scar less than dermal tissue, which may be due to the elements in saliva and unique phenotype of oral fibroblasts ( 14 - 16 ). (spandidos-publications.com)
  • As a result, cardiomyocytes were unable to efficiently pump blood throughout the body. (defytime-emea.com)
  • On the left are processes that occur as a result of being ill and hospitalized or receiving treatment. (cdc.gov)
  • Nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1 expression significantly increased in IMI rats and IMI rats that received PN. (hindawi.com)
  • In the animal model, palatal wound closure was significantly enhanced in rats treated with Tβ4. (spandidos-publications.com)
  • MSCs significantly reversed the imbalance of glycolysis in sperm and testis induced by testicular torsion-detorsion, as evidenced by increasing the expression of phosphoglycerate kinase 2 and glyceraldehyde-3-phosphate dehydrogenase-spermatogenic, activating Akt, and increasing glycogen synthase kinase 3 (GSK3), which led to the increase in glycolysis cascades and ATP production. (biomedcentral.com)
  • Furthermore, it was shown that a reduction in heme oxygenase (HO)-1 levels and an induction of endogenous antioxidants occurred via the activity of nuclear factor erythroid 2-related factor 2 (Nrf2) [ 5 ]. (hindawi.com)
  • After cardiomyocytes were treated with imatinib, they expressed enhanced Ca2+ activity. (temple.edu)
  • If caught and treated early, disease progression in HH can be altered significantly. (medscape.com)
  • Intact cardiomyocytes isolated from mutant hearts exhibited little difference in shortening or Ca2+ transient magnitudes but significantly slowed kinetics of both contraction and relaxation. (ox.ac.uk)
  • Within the last 20 years, our understanding of how conduction works has significantly changed, but our understanding of excitability has not. (vt.edu)
  • Cardiomyocytes were randomly assigned to the control group, ISO group (100 nM ISO for 48 h), Res + ISO group (50 μM Res and 100 nM ISO for 48 h) and Res group (50 μM Res for 48h only). (karger.com)
  • Depending on response, administer at increments of 20-40 mg, no sooner than 6-8 h after previous dose, until desired diuresis occurs. (medscape.com)
  • Thrombosis occurs when thrombi occlude vessels in the body. (wikipedia.org)