• We analyzed standard reference RNA samples and RNA samples obtained from human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs). (biomedcentral.com)
  • Current methods for the maturation of induced pluripotent stem cell-derived cardiomyocytes. (wjgnet.com)
  • Suppression of the pathway promoted emergence of reparative macrophages, thereby mitigating pathological ventricular remodeling. (libsyn.com)
  • Through in-depth exploration of macrophages, recent research has shown that the phenotype of macrophages exhibits greater plasticity than previously assumed [ 3 ]. (thno.org)
  • The relationship between monocyte subset and macrophage phenotype is still to be resolved, however tissue macrophages clearly have diverse functions and are key inflammatory mediators [ 23 ]. (biomedcentral.com)
  • Rationale: Pathological hypertrophy commonly leads to heart failure (HF) and loss of cardiomyocytes, while physiological hypertrophy protects the heart and enhances cardiomyogenesis. (researchgate.net)
  • Hypertrophic cardiomyopathy (HCM) is defined by ventricular hypertrophy. (cdc.gov)
  • However, the broader phenotype includes abnormal cardiomyocyte orientation (disarray), myocardial ischaemia and electrical abnormalities, which seem to manifest before overt hypertrophy. (cdc.gov)
  • higher sleep blood pressure levels are associated with target organ damage (including higher left ventricular mass and left ventricular hypertrophy) and that masked nocturnal hypertension is associated with incident hypertension among African Americans. (the-asci.org)
  • Hypertrophic cardiomyopathy (HCM) is the most common heritable cardiovascular disorder and is characterized by left ventricular hypertrophy (LVH), which is unexplained by abnormal loading conditions. (mdpi.com)
  • The pathological consequences associated with normal cardiovascular aging include hypertrophy, altered left ventricular (LV) diastolic and systolic function, heart failure, enhanced arterial stiffness, and endothelial dysfunction, all of which can alter the structure and function of the heart and arterial system [ 5 , 6 ] . (oaepublish.com)
  • They found that current cigarette smoking status, smoking intensity in terms of cigarettes per day, and smoking burden in pack-years, were independently associated with higher left ventricular mass, lower left ventricular strain, higher brain natriuretic peptides, higher BNP levels and higher risk of incident heart failure hospitalization in blacks. (libsyn.com)
  • Mice with altered expression of N-cadherin and/or E-cadherin showed a dilated cardiomyopathy phenotype, likely due to malfunction of intercalated discs. (wikipedia.org)
  • In agreement with this, mice with ablation of N-cadherin in adult hearts via a cardiac-specific tamoxifen-inducible Cre N-cadherin transgene showed disrupted assembly of intercalated discs, dilated cardiomyopathy, impaired cardiac function, decreased sarcomere length, increased Z-line thickness, decreases in connexin 43, and a loss in muscular tension. (wikipedia.org)
  • Dilated cardiomyopathy (DCM) is a highly morbid condition affecting relatively young and active adults. (escardio.org)
  • We demonstrated that fully functional EHTs could be generated from physiologically relevant combinations of hiPSC-derived cardiomyocytes (70%), cardiac fibroblasts (15%) and cardiac endothelial cells (15%), using as few as 1.6 × 104 cells. (bvsalud.org)
  • The heart is a complex organ made of various cell types including cardiomyocytes, endothelial and smooth muscle cells, but also fibroblasts and inflammatory cells, which could all be implicated in the disease process. (escardio.org)
  • Surprisingly, despite the presence of increased fibrosis, fibroblasts are not over-represented in neither right- nor left-ventricular samples of DCM patients, supporting a phenotypic shift of DCM fibroblasts towards a secretory rather than a proliferative phenotype. (escardio.org)
  • Furthermore, cell clustering analysis based on expression mapping provided a molecular signature of all the cell types present in the diseased hearts including cardiomyocytes, fibroblasts, smooth muscle cells, pericytes, inflammatory (myeloid and lymphoid) as well as neuronal and adipose cells, providing an incredible resource for future research in the field. (escardio.org)
  • We quantified CXCL10 in human CKD plasma and tested the response of human iPSC-derived cardiomyocytes and primary cardiac fibroblasts to serum from CKD donors. (biomedcentral.com)
  • In humans, we found increased plasma CXCL10 concentrations in advanced CKD, and identified the production of CXCL10 in cardiomyocytes and cardiac fibroblasts. (biomedcentral.com)
  • Despite substantial declines in mortality following myocardial infarction (MI), subsequent left ventricular remodeling (LVRm) remains a significant long-term complication. (researchgate.net)
  • Myocardial infarction results in the loss of ventricular heart tissue which is not efficiently replaced [ 1 ]. (biomedcentral.com)
  • Left ventricular wall segment motion after ultra-endurance exercise in humans assessed by myocardial speckle tracking. (ljmu.ac.uk)
  • Transplantation of embryonic cardiomyocytes after extensive MI in a rat model attenuate LV dilatation, infarct thinning, and myocardial dysfunction. (nih.gov)
  • Recently, EndMT has been increasingly recognized as a vital process that contributes to various cardiovascular pathologies in adults, which include atherosclerosis, valvular heart disease, cardiac fibrosis, and myocardial infarction. (dovepress.com)
  • Ventricular remodeling (VR) is essential for the progression from acute myocardial infarction (AMI) to heart failure [ 1 ]. (thno.org)
  • Preload represents the degree of end-diastolic fiber stretch and end-diastolic volume, which is influenced by ventricular diastolic pressure and the composition of the myocardial wall. (msdmanuals.com)
  • Decreased Expression of Plakophilin-2 and αT-Catenin in Arrhythmogenic Right Ventricular Cardiomyopathy: Potential Markers for Diagnosis. (nih.gov)
  • Population Prevalence of Premature Truncating Variants in Plakophilin-2 and Association With Arrhythmogenic Right Ventricular Cardiomyopathy: A UK Biobank Analysis. (nih.gov)
  • The syndrome is caused by changes in the structure and function of certain cardiac ion channels and reduced expression of Connexin 43 (Cx43) in the Right Ventricle (RV), predominantly in the Right Ventricular Outflow Tract (VSVD), causing electromechanical abnormalities. (bvsalud.org)
  • Our global hypothesis is that by manipulating the cardiac gene program of the failing heart with soluble factors (drugs), we may augment endogenous and/or transplanted cardiac myocyte regeneration to ameliorate the progression of left and/or right ventricular failure. (ucdavis.edu)
  • The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
  • Her research focuses on hypertension diagnosis, treatment, out-of-office blood pressure monitoring, and the characterization of several blood pressure phenotypic measures including awake and sleep blood pressure, and abnormal ambulatory blood pressure phenotypes including white coat hypertension and masked hypertension. (the-asci.org)
  • enhanced secretion of adiponectin by cardiomyocytes that is stimulated by BNP (brain natriuretic peptide), disturbed metabolism of adiponectin in older patients with CVD as well as changes in multimerization of adiponectin. (czytelniamedyczna.pl)
  • Transcription factor GATA4-targeted compounds that have previously shown in vivo efficacy in cardiac injury models were tested for stage-specific activation of atrial and ventricular reporter genes in differentiating pluripotent stem cells using a dual reporter assay. (biomedcentral.com)
  • Failure to induce critical genes involved in contractile function, calcium handling and energy metabolism underpins this common phenotype. (ox.ac.uk)
  • RBM20 binds directly to the primary RNA (pre-mRNAs) of many cardiomyopathy-associated genes, where it ensures the proper production of adult protein isoforms via an exon exclusion process (i.e., splice isoforms associated with cardiac maturation). (hilarispublisher.com)
  • In this review, we focus on the role of cardiomyocyte-derived and cardiac fibroblast-derived microRNAs that are involved in the regulation of genes associated with cardiomyocyte and fibroblast function and in atherosclerosis-related cardiac ischemia. (archivesofmedicalscience.com)
  • We then validated the model in both primary mouse- and human pluripotent (embryonic) stem cell-derived cardiomyocytes showing that field potentials measured in MEAs could be converted to action potentials that were essentially identical to those determined directly by electrophysiological patch clamp. (bvsalud.org)
  • Many of them, however, kept their embryonic phenotype and were isolated from the host myocardium by scar tissue. (nih.gov)
  • To elucidate the role of glucocorticoid signalling in late-gestation cardiovascular maturation, we have generated mice with conditional disruption of glucocorticoid receptor (GR) in cardiomyocytes and vascular smooth muscle cells using smooth muscle protein 22-driven Cre recombinase (SMGRKO mice) and compared them with mice with global deficiency in GR (GR(-/-)). Echocardiography shows impaired heart function in both SMGRKO and GR(-/-) mice at embryonic day (E)17.5, associated with generalized oedema. (ox.ac.uk)
  • Embryonic and follicular forms occur in young patients and are collectively referred to as juvenile RMS, while pleomorphic forms occur mainly in adults. (biomedcentral.com)
  • Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)
  • Echocardiography study was performed before and 53+/-3 days after implantation to assess left ventricular (LV) remodeling and function. (nih.gov)
  • Rats injected with ISO exhibited severe interstitial cardiac fibrosis and perivascular fibrosis, decreased left ventricular ejection fraction, and increased NOX activity. (dovepress.com)
  • TMZ treatment mitigated cardiac fibrosis, ameliorated left ventricular dysfunction, and reduced NOX activity. (dovepress.com)
  • Performing single cell sequencing of cardiac right- and left-ventricular samples obtained from DCM and ACM patients, Reichart et al. (escardio.org)
  • provided for the first time a comprehensive cellular atlas of the DCM and ACM hearts, managing to obtain data from 500000 nuclei of patients and 380000 nuclei from controls.5 By clustering the cells based on their expression profile, by stratifying expression analysis by genotype and by comparing right and left ventricular samples, the authors provide an impressive set of data. (escardio.org)
  • They examined the relationship between cigarette smoking and left ventricular strength and function by using cardiac magnetic resonance imaging. (libsyn.com)
  • After heart transplantation, the donor heart preservation with normoxic CM was associated with shorter re-beating time, histopathological scores, and left ventricular systolic diameter, higher ejection fraction, and fractional shortening of transplanted hearts. (researchsquare.com)
  • Zurück zum Zitat Damy T, Costes B, Hagège AA et al (2016) Prevalence and clinical phenotype of hereditary transthyretin amyloid cardiomyopathy in patients with increased left ventricular wall thickness. (springermedizin.de)
  • Non-ischemic cardiomyopathy (NICM) can cause left ventricular dysfunction through interstitial fibrosis, which corresponds to the failure of cardiac tissue remodeling. (nature.com)
  • Typically, left ventricular (LV) end-diastolic pressure, especially if higher than normal, is a reasonable measure of preload. (msdmanuals.com)
  • Heart failure (HF) is a syndrome of ventricular dysfunction. (msdmanuals.com)
  • Chemical compounds that modulate atrial and ventricular cell fate could be used to improve subtype-specific differentiation of endogenous or exogenously delivered progenitor cells in order to promote cardiac regeneration. (biomedcentral.com)
  • Vascular endothelial cell (EC)-derived factors play an important role in endothelial-cardiomyocyte crosstalk and could save cardiomyocytes (CMs) from injury. (mdpi.com)
  • Mice died within two months of transgene expression, mainly due to spontaneous Ventricular tachycardia. (wikipedia.org)
  • Other heart problems that occur in ankyrin-B syndrome include irregular and uncoordinated electrical activity in the heart's upper chambers (atrial fibrillation) or lower chambers (ventricular fibrillation) and an abnormality called catecholaminergic polymorphic ventricular tachycardia (CPVT), in which an increase in the heart rate can trigger an abnormally fast and irregular heartbeat called ventricular tachycardia . (medlineplus.gov)
  • Mutations in this gene are associated with different inherited cardiac conditions including Arrythmogenic Cardiomyopathy, Brugada Syndrome, and Idiopathic Ventricular Fibrillation. (nih.gov)
  • Furthermore, RBM20 DCM is highly penetrant, linked to life-threatening ventricular arrhythmias, and manifests at a younger age than DCM caused by mutations in other proteins (e.g., laminA/C or Titin). (hilarispublisher.com)
  • 2 EndMT is a process by which endothelial cells lose a portion of their cellular features and obtain the mesenchymal phenotype, including the loss of tight junctions and increased production of extracellular matrix. (dovepress.com)
  • Filling an important gap between genomic and expression data, those single cell analyzes show that DCM and ACM are characterized by a depletion in cardiomyocytes (except in LMNA cardiomyopathy), and an enrichment in endothelial and inflammatory cells (myeloid and lymphoid cells). (escardio.org)
  • Amend N, Thiermann H, Worek F, Wille T ( 2019 ) The arrhythmogenic potential of nerve agents and a cardiac safety profile of antidotes - A proof-of-concept study using human induced pluripotent stem cells derived cardiomyocytes (hiPSC-CM). Toxicol Lett 308:1-6. (multichannelsystems.com)
  • Kraushaar U, Guenther E ( 2019 ) Assay Procedures for Compound Testing of hiPSC-Derived Cardiomyocytes Using Multiwell Microelectrode Arrays. (multichannelsystems.com)
  • Tahrir FG, Gordon J, Feldman AM, Cheung J, Khalili K, Mohseni Ahooyi T ( 2019 ) Evidence for the impact of BAG3 on electrophysiological activity of primary culture of neonatal cardiomyocytes. (multichannelsystems.com)
  • There is a growing body of evidence which demonstrates that following ACS, microRNAs might inhibit fibroblast proliferation and scarring, as well as harmful apoptosis of cardiomyocytes, and stimulate fibroblast reprogramming into induced cardiac progenitor cells. (archivesofmedicalscience.com)
  • Due to cellular heterogeneity in the heart, the activation of gene programs representing specific atrial and ventricular cardiomyocyte subtypes would be highly desirable. (biomedcentral.com)
  • GATA4-targeted compounds 3i-1000 and 3i-1103 were identified as differential modulators of atrial and ventricular gene expression. (biomedcentral.com)
  • Furthermore, it is unknown whether endogenous progenitors/stromal cells in the adult human heart could be chemically induced to generate functional atrial or ventricular heart muscle to treat adult heart diseases. (biomedcentral.com)
  • Expression of a mutant form of Cadherin-2 harboring a large deletion in the extracellular domain inhibited the function of endogenous Cadherin-2 in adult ventricular cardiomyocytes, and neighboring cardiomyocytes lost cell-cell contact and gap junction plaques as well. (wikipedia.org)
  • Recently, in combination with cardiomyocytes derived from human (induced) pluripotent stem cells they have started to become a preferred tool to examine newly developed drugs for potential cardiac toxicity in pre-clinical safety pharmacology. (bvsalud.org)
  • Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
  • The most valuable cells for regenerative endodontics are postnatal or adult stem cells. (bvsalud.org)
  • These data demonstrate that structural, functional and biochemical maturation of the foetal heart is dependent on glucocorticoid signalling within cardiomyocytes and vascular smooth muscle, though some aspects of heart maturation (size, ANP expression) are independent of GR at these key sites. (ox.ac.uk)
  • Glucocorticoids are vital for the structural and functional maturation of foetal organs, yet excessive foetal exposure is detrimental to adult cardiovascular health. (ox.ac.uk)
  • Referenced article focusing on the relationship between phenotype and genotype. (cancerindex.org)
  • The enormous variants and mutations found mean that we are still far from being able to concretely clarify a genotype-phenotype relationship. (bvsalud.org)
  • In failing cardiomyocytes, from both rodents and humans, this transverse tubule coupling between LTCC and β2AR was lost. (imperial.ac.uk)
  • Extracellular small non-coding RNAs (exRNAs) have been associated with cardiac inflammation and fibrosis and we hypothesized that they are associated with post-MI LVRm phenotypes. (researchgate.net)
  • Collectively, these results indicate the potential for therapeutic alteration of cell fate decisions and pathological gene regulatory networks by GATA4-targeted compounds modulating chamber-specific transcriptional programs in multipotent cardiac progenitor cells and cardiomyocytes. (biomedcentral.com)
  • Cardiac ultrastructure is markedly disrupted in both SMGRKO and GR(-/-) mice at E17.5, with short, disorganized myofibrils and cardiomyocytes that fail to align in the compact myocardium. (ox.ac.uk)
  • (M-P) Ventral view with optical sections taken at the same Z-position from SPIM imaging show in tbx5a frameshift mutants the inflated pericardial space surrounding the heart and thinner myocardium, floating ventricles, and looping defects (N-P) compared to wildtype (M). (Q-T) Corresponding lateral view of maximum intensity projections from panels (M-P) shown for blue channel (myl7:AmCyan, marking cardiomyocytes). (sciencematters.io)
  • The most important risk parameter is proarrhythmic activity in cardiomyocytes which can cause sudden cardiac death. (bvsalud.org)
  • In cardiac muscle, Cadherin-2 is an integral component in adherens junctions residing at intercalated discs, which function to mechanically and electrically couple adjacent cardiomyocytes. (wikipedia.org)
  • With an estimated prevalence of about 1/250, DCM is one of the leading causes of heart transplantation in the world.1,2 At the morphological level, DCM is characterized by ventricular dilatation, cardiomyocyte loss and fibrotic replacement, leading to impaired contractility, heart failure, arrhythmia and conduction disorders. (escardio.org)
  • In patients under optimal medical therapy with moderate to severe HF and cardiac dyssynchrony, cardiac resynchronization therapy (CRT) with a defibrillator improves contractile function and reverse ventricular remodeling, ameliorating symptoms, quality of life (QoL), and clinical outcomes [ 7 , 8 ]. (hindawi.com)
  • Background:Beta-2 adrenergic receptors (β2ARs) but not beta-2 adrenergic receptors (β1ARs) form a functional complex with L-type Ca2+ channels (LTCCs) on the cardiomyocyte membrane. (imperial.ac.uk)
  • We aim to study the coupling between LTCC and β adrenergic receptors in different cardiomyocyte microdomains, the distinct involvement of PKA and CAMKII (Ca2+/calmodulin-dependent protein kinase II) and explore how this functional complex is disrupted in heart failure.Methods:Global signaling between LTCCs and β adrenergic receptors was assessed with whole-cell current recordings and western blot analysis. (imperial.ac.uk)
  • More detailed structure-function analysis revealed a distinct subclass of GATA4/NKX2-5 inhibitory compounds with an acetyl lysine-like domain that contributed to ventricular cells (%Myl2-eGFP+). (biomedcentral.com)
  • Data are limited on neurocognitive function in adults with CHD (ACHD). (stanford.edu)
  • The first original paper this week provide experimental data showing that the endothelium controls cardiomyocyte metabolism and function via notch signaling. (libsyn.com)
  • Interestingly, those expression data reinforce the role of common variants in modulating DCM phenotype. (escardio.org)
  • Importance Mortality is high among patients heart failure (HF) who are receiving treatment, and therefore identifying new pathways rooted in preclinical cardiac remodeling phenotypes may afford novel biomarkers and therapeutic avenues. (researchgate.net)
  • Loss of cardiomyocytes is a major cause of heart failure, and while the adult heart has a limited capacity for cardiomyogenesis, little is known about what regulates this ability or whether it can be effectively harnessed. (researchgate.net)
  • Ventricular larger signs in the lower advances of organic women Additionally have pacemakers that, under Trends of great failure, delete variety of loss specifically not toward the selection. (augenta.net)
  • Nonetheless, distinct from hst mutants, homozygous and trans-heterozygous combinations of these tbx5a frameshift mutants do not readily manifest the stretched hst heart phenotype. (sciencematters.io)
  • Writzl et al reported a family with molecularly proven (p.Thr468Met in PTPN11) LEOPARD syndrome in a father and his adult son. (medscape.com)
  • Long QT syndrome (LQTS) is a congenital disorder characterized by a prolongation of the QT interval on electrocardiograms (ECGs) and a propensity to ventricular tachyarrhythmias, which may lead to syncope, cardiac arrest, or sudden death. (medscape.com)
  • To define this communication, a uncontrolled degree phosphorylation of devoted stomach which improves double current myocytes filed in Intracellular goals and new experience database with a recombinant synchrony with acromegalic patients organized with sub-cellular microorganisms of summary will determine been by induction duration adult. (augenta.net)
  • While some people with HCM are very sick, many people, especially children, teens, and young adults, have no or few symptoms and may not know they have a heart problem. (cdc.gov)