Cardiomyocytes cultures embryonic cell differentiation. Medical search. Frequent questions

At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)
Stem cell-derived cardiac myocytes are potential sources for testing cardiocytoprotective molecules against ischemia/reperfusion injury in vitro. (nih.gov)
Mouse ES cells have been widely utilized as an in vitro model to study cardiogenesis, as cardiomyocytes were found to spontaneously differentiate from ES cells after withdrawal of LIF (leukemia inhibitory factor), which functions to maintain the pluripotency of undifferentiated mouse ES cells [ 2 - 4 ]. (biomedcentral.com)
Here, we investigated the role of circ-JA760602 in modulating the apoptosis of hypoxia-induced AMI cells using the AC16 cardiomyocyte in vitro cell model. (ehu.eus)
Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for prolonged periods of time [ 2 ] supporting the notion that entire organs could be cultured in vitro. (intechopen.com)
A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
This protocol introduces how to generate heart organoids from mouse embryonic stem cell-derived embryoid bodies (EB) in the presence of the laminin-entactin (LN/ET) complex and fibroblast growth factor 4 (FGF4) by three consecutive steps, 1) the culture of ESCs, 2) in vitro differentiation of ESCs into EBs and 3) in vitro culture of EBs for the generation of heart organoids. (researchsquare.com)
For 3D culture methods using embryonic stem cells (ESCs) or adult stem cells, ECMs including Matrigel and laminin were widely used for in vitro organogenesis,including the development of the brain 1 , stomach 2 , kidney 3 , and intestinal tissues 4 . (researchsquare.com)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
EpiMOGRIFY-predicted factors are able to maintain astrocytes and cardiomyocytes in vitro in chemically defined media and promote the generation of astrocytes and cardiomyocytes from neural progenitors and embryonic stem cells, respectively. (technologynetworks.com)
Therefore, the implantation of human cells reprogrammed in vitro represents an alternative strategy [ 16 - 19 ]. (thno.org)
In vitro toxicity data of these metabolites derived in the development of validated and accepted in vitro and in silico embryonic stem cell test were used as input in the PBK model to extrapolate in vitro concentration-response curves to predicted approaches is urgently needed. (cdc.gov)
We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)

The surgical procedure for delivery of embryonic stem cell-derived endothelial cells to the ischemic hindlimb is demonstrated, with non-invasive tracking by bioluminescence imaging. (jove.com)
Nowadays, more and more studies have revealed that cells in the cardiovascular system (such as cardiomyocytes, vascular smooth muscle cells, endothelial cells, et al. (frontiersin.org)
First, we cocultured dermal fibroblasts with dermal microvascular endothelial cells at a ratio of 2 : 1. (hindawi.com)
A fibrous sheet was formed by the interactions between the fibroblasts and the endothelial cells, and capillary-like structures were observed after 20 days of coculture. (hindawi.com)
ELISA demonstrated that vascularization was promoted by significant upregulation of vascularization associated growth factors due to interactions among the 3 types of cells in the bilayer, as compared to cocultures of fibroblast and endothelial cells and monocultures. (hindawi.com)
Most of these methods involve culturing endothelial cells on or within ECM substrates (e.g., collagen, fibrin, fibronectin, laminin, etc.) or other types of biomaterials to form capillary-like structures [ 15 ]. (hindawi.com)
Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
Mammalian heart development involves differentiation of diverse cell types, including atrial and ventricular muscle cells known as cardiomyocytes (CMs), specific myocytes constituting conducting tissue such as Purkinje fibers, smooth muscle (SM) cells, and non-muscle cells including endothelial cells (ECs) and neuronal cells. (researchsquare.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)

A past research shows that overexpression of TBX5 GATA4 and MEF2C transcription elements in cardiac fibroblasts could create cardiomyocytes [17]. (bibf1120.com)
Herein, we introduce a cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming. (thno.org)
The human neonatal dermal fibroblasts containing cardiac transcription factors were plated on the membrane and cultured with the murine cardiomyocyte in the presence of the electric stimulus. (thno.org)
Nano-thin and nano-porous membranes in the culture system facilitated interactions between fibroblasts and cardiomyocytes in coculture. (thno.org)
The cellular interactions and electric stimulation supplied by the culture system dramatically enhanced the cardiac reprogramming efficiency of cardiac-specific transcriptional factor-transfected fibroblasts. (thno.org)
The cardiac-mimetic culture system may serve as an effective tool for producing a feasible number of reprogrammed cardiomyocytes from fibroblasts. (thno.org)
iPSC, derived from human fibroblasts and osteoblasts, have been successfully differentiated into hepatocytes, cardiomyocytes, and neural crest cells. (lnhlifesciences.org)
One way we did this was by converting adult fibroblasts, or skin cells, into interneurons. (lu.se)

Under these conditions, iPS cells begin to differentiate into a hepatocyte lineage. (spandidos-publications.com)
Laminin-221 (LN221) was identified as the most likely expressed cardiac laminin and the authors show that this protein promotes differentiation of pluripotent human embryonic stem cells (hESCs) toward cardiomyocyte lineage and downregulates pluripotency and teratoma-associated genes. (biolamina.com)
This work reveals the importance of BRACHYURY and CDX2 genes as key mediators of embryonic and extraembryonic lineage differentiation in hESCs and EpiSCs. (qscience.com)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)

Reprogramming triggers a cascade of evident changes in the host cells that are recognizable morphologically and through a combination of markers and pluripotency assays. (cellapplications.com)
In recent studies we have examined the role of transforming growth factor family members in both pluripotency and differentiation. (qscience.com)
EpiSCs share many features with hESCs, and subsequent work supports our hypothesis that hESCs are the human counterparts of EpiSCs, with similar responses to growth factors and mechanisms of pluripotency and differentiation. (qscience.com)
Promotes self-renewal and maintains pluripotency of human embryonic stem cells and mouse Epi-stem cells when used in combination with CHIR99021 (Kim et al. (stemcell.com)
Embryonic stem cells (ESCs) possess the capacity to create any kind of cell in the torso because of its pluripotency in character [4]. (bibf1120.com)

Embryoid-body-like structures were formed after 6 days with 1 μM of 5-aza in a P19 cell monolayer culture, beating cell clusters first observed on day 12, and, the production of beating cell clusters increased by 80.1% (29 of 36-wells) after 18 days. (korea.ac.kr)
This study has demonstrated that 5-aza induces differentiation of P19 cells into cardiomyocytes in a confluent monolayer culture in the absence of prior embryoid formation and dimethyl sulfoxide exposure, depending in part on alteration of BMP signaling molecules. (korea.ac.kr)
After analysis of full embryoid bodies (EBs) and cardiac marker (VCAM and cardiac troponin I) positive cells of three lines at 6 conditions (32 different conditions altogether), we found significant SI/R injury-induced cell death in both full EBs and VCAM+ cardiac cells at later stage of their differentiation. (nih.gov)
ES cells were aggregated into three-dimensional structures, termed embryoid bodies (EBs), and suspended in media containing fetal calf serum. (biomedcentral.com)
These human iPSC derivatives were differentiated through embryoid body formation and then adherent culture on LifeSciences' proprietary human-derived extracellular matrix (ECM), Hu Gentra TM . (lnhlifesciences.org)

During gastrulation, as the epiblast cells migrate through the primitive streak, they undergo epithelial-mesenchymal transition and form the mesoderm. (mechanobio.info)
Our analysis showed that Sox4 , Sox5 , Sox9 , Sox11 , and Sox12 are mainly expressed in mesenchymal stem/stromal cells (MSCs) representing osteogenic cells at different stages of differentiation. (ehu.eus)
These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)

Promotes differentiation of cardiomyocytes from human pluripotent stem cells (PSCs) that have been induced to mesoderm by addition of Activin A and/or BMP4 (Ren et al. (stemcell.com)
Time-lapse videography of living cells captures spectacular intracellular mitochondrial movement, and this movement promotes mitochondrial connections to form a dynamic mitochondrial network [ 3 ]. (biomedcentral.com)

In addition, we summarize the recent direct reprogramming of cardiomyocytes from fibroblast cells, which provides another method for potential heart disease therapy. (biomedcentral.com)
On the mobile level, cardiac redecorating is seen as a cardiomyocyte hypertrophy, fibroblast hyperplasia associated with a rise in collagen deposition inside the interstitial matrix (fibrosis) and cell loss of life. (researchassistantresume.com)

HiPSC differentiate into cell derivatives from the 3 embryonic layers: Neuronal marker beta III tubulin (TUJ1), Smooth Muscle Actin (SMA) and Hepatocyte Nuclear Factor 3 Beta (HNF3b). (cellapplications.com)

One of promising sources of cardiomyocytes is embryonic stem cells (ESCs) and induced pluripotent stem cells (IPSCs). (edu.ua)
A prior study implies that ESCs could actually generate cardiomyocytes and acquired limited death count within a rat center ischemia model [5]. (bibf1120.com)

The effect of dimethylsulfoxide (DMSO) on the processes of differentiation of pluripotent stem cells into cardiomyocytes was studied both at the stage of mesoderm cells formation and at the stage of the cardiomyocytes progenitors formation. (edu.ua)
During stem cell cardiac differentiation, a distinct swish in the expression profile of laminin subunits has been reported with laminin-411/421 pre-dominantly expressed early in progenitors and laminin-211/221 expressed later in cardiomyocytes (Ja, 2015). (biolamina.com)
A highly reproducible, chemically defined, xeno-free laminin-based differentiation protocol to generate stem cell-derived cardiovascular progenitors (CVPs) was reported in 2019 (Yap, 2019). (biolamina.com)
Laminin-521 also has properties that improve the isolation and expansion of adult stem cells and progenitors. (biolamina.com)

Studies have shown that cell-to-cell mitochondrial transfer plays an essential role in regulating cardiovascular system development and maintaining normal tissue homeostasis under physiological conditions. (frontiersin.org)
Cell transplantation is a modem strategy for injured heart tissue regeneration. (edu.ua)
I returned full time to Imperial College in 1999 to take up a lectureship in cell biology and tissue engineering and was also actively involved in establishing the Tissue Engineering Centre. (nottingham.ac.uk)
I was also affiliated with the Wolfson Centre for Stem Cells, Tissue Engineering and Modelling (STEM), part of the Centre for Biomolecular Sciences, which is now the Biodiscovery Institute. (nottingham.ac.uk)
My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. (nottingham.ac.uk)
Epithelial cells were then seeded on the fibrous sheet to assemble the bilayered tissue. (hindawi.com)
Thus, the long-term survival and function of 3D tissues depend on the rapid development of new blood vessels to provide nutrients and oxygen to cells in the center of the tissue grafts. (hindawi.com)
Adjustments in RA homeostasis (missing or more than RA) bring about serious malformations during cardiogenesis, recommending that a specific tissue focus of RA can be indispensable for the correct induction of signaling pathways very important to regular myocardial cell development and differentiation in early embryonic levels. (researchassistantresume.com)
The general underlying strategy behind the development of tissue engineered organ substitutes is the utilization of a combination of cells, biomaterials, and biochemical cues intended to recreate the natural organ environment. (biomedcentral.com)
The purpose of this review is to highlight current bioengineering approaches for salivary gland tissue engineering and the adult stem cell sources used for this purpose. (biomedcentral.com)
This article is intended to provide a review of the most current approaches to utilizing stem cells and bioengineering principles for the purpose of salivary tissue regeneration. (biomedcentral.com)
Critical to the hypothesis is the experimental fact that human endometrial-derived stem cells (hEnSCs) as multipotent accessible source of cells are known as useful cell candidates in the field of nerve tissue engineering. (scirp.org)
These novel differentiated cells in fibrin gel might open new opportunities for the management of cell survival and neurotrophic potential in tissue engineering approaches for nerve repair. (scirp.org)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
The platform utilizes data from more than 100 human cell/tissue types (available via the ENCODE and Epigenome Roadmap consortia) to accurately define culture conditions that can maintain the cell identity or induce cell conversion. (technologynetworks.com)
An immunologically identical cardiac tissue can be produced by direct reprogramming from the patients' tissue without the potential risk of tumorigenesis that occurs with induced pluripotent stem cells [ 2 ] and the ethical issue using embryonic stem cells. (thno.org)
The relevance of these phenomena in stem cell biology and tissue repair is discussed. (unicyte.ch)
The success of these cell models on human tissue-derived ECM will further advance discovery and development of new therapies. (lnhlifesciences.org)
The key elements of tissue engineering are stem cells, morphogen, and a scaffold of extracellular matrix. (bvsalud.org)
Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)

Cell Characterization. (cellapplications.com)
Hence energetic characterization is necessary before presenting any cell for dealing with targeted diseases. (bibf1120.com)

Kraushaar U, Guenther E ( 2019 ) Assay Procedures for Compound Testing of hiPSC-Derived Cardiomyocytes Using Multiwell Microelectrode Arrays. (multichannelsystems.com)
Cell viability was measured by cell counting kit-8 (CCK-8) assay. (ehu.eus)
Apoptosis of cardiomyocytes was evaluated by TUNEL assay and flow cytometry analysis. (ehu.eus)
showed that the differentiation assay of benchmark dose. (cdc.gov)

HiPSC-derived Neural Stem Cells (L) and Neurons (R) . i-HNSC stained w/ Nestin (neural stem cell marker, green), SOX 2 (stem cell marker, red) & DAPI (nuclear stain, blue). (cellapplications.com)
The idea that cellular survival and growth could be maintained outside the body was recognized as possible almost hundred years ago when the German zoologist Wilhelm Roux described a successful experiment where he cultured chick neural crest in warm saline for a few days [ 1 ]. (intechopen.com)

In response to 1 μM of 5-aza, P19 cells expressed bone morphogenetic protein-2 (BMP-2), BMP-4, Bmpr1a and Smad1 at day 6 or 9, and also cardiac markers such as GATA-4, Nkx2.5, cardiac troponin I, and desmin were up-regulated in a time-dependent manner after induction of BMP signaling molecules. (korea.ac.kr)
Moreover, full EBs of the iPS 4.1 cell line after oxidative stress induction by SNAP was protected at day-8 samples. (nih.gov)
In our most recent work we have focused on the role of bone morphogenetic protein (BMP)-4 in the cell fate decision between endoderm and mesoderm, demonstrating the similarity of BMP-induced hESC and EpiSC differentiation to mesoderm induction during mouse gastrulation. (qscience.com)
In this study, we investigate the expression of differentiated Schwann cell markers by exposing of endometrial stem cells with induction media including FGF2/FSK/HRG/RA. (scirp.org)
Further induction into cardiomyocyte uncovered ASC expressing more considerably cardiomyocyte particular markers in comparison to SHED through the differentiation training course evidenced by morphology and gene appearance profile. (bibf1120.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)

In the earliest stages of development, the physical properties of the microenvironment can direct cell differentiation, and initiate the coordinated movement of groups of cells to establish the patterns that will define how the body is arranged. (mechanobio.info)
The ability to systematically identify the optimal culture conditions, closely mimicking the in vivo microenvironment, will enable scientists to convert and maintain any cell type more efficiently and with even greater accuracy. (technologynetworks.com)
The cell phenotype is therefore determined by signals that target the cells received within a defined microenvironment. (unicyte.ch)

We also discussed the advantage and challenges of mitochondrial transfer strategies, including cell-based mitochondrial transplantation, extracellular vesicle-based mitochondrial transplantation, and naked mitochondrial transplantation, for the treatment of cardiovascular disorders. (frontiersin.org)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
Role of extracellular RNA-carrying vesicles in cell differentiation and reprogramming. (unicyte.ch)
In the present review we focus on the extracellular vesicle-induced epigenetic changes in recipient cells that may lead to phenotypic and functional modifications. (unicyte.ch)
In particular, stem cells are highly sensitive to extracellular signals that play a critical role in mainten- ance of stem cell characteristics, differentiation, and interplay with somatic cells. (unicyte.ch)
Similarly, reprogramming of somatic cells involves a complex interaction among intracellular and extracellular signals leading to epigenetic remodeling [6]. (unicyte.ch)

The generated heart organoids possess structural and functional capacity similar to atrial and ventricular parts of in vivo embryonic heart. (researchsquare.com)

RA signaling continues to be implicated within the legislation of cell differentiation, proliferation and apoptosis [116,117], and a considerable body of understanding has gathered on its function in the legislation of cardiomyocyte development, apoptosis and mobile function in response to different pathophysiological stimuli. (researchassistantresume.com)
We reported that silence of lncRNA GAS5 attenuated hypoxia-triggered cell death, as cell viability was increased and apoptosis rate was decreased. (molcells.org)
In this study, we aimed to analyze the role of Dnmt1 and Dnmt3a enzymes in ICM (using the Nanog marker) and TE (using the Cdx2 marker) differentiation, autophagy (using p62 marker), reactive oxygen species (ROS) production, and apoptosis (using TUNEL) during mouse preimplantation embryo development. (ehu.eus)
Following knockdown of Dnmt1 and Dnmt3a in zygotes, expression levels of Cdx2 in the trophectoderm and Nanog in the inner cell mass were measured, as well as p62 levels, reactive oxygen species (ROS) production, and apoptosis levels after 96 hours in embryo culture. (ehu.eus)

Our focus on mesoderm leads us on to molecular pathways for early human cardiomyocyte differentiation, with a goal of understanding the transcriptional networks responsible for cardiomyocyte identity and using this to generate more homogeneous cardiomyocyte populations for therapeutic applications and drug discovery. (qscience.com)
Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
Revealing cell populations catching the early stages of the human embryo development in naïve pluripotent stem cells. (axonmedchem.com)
11 , 12 Light-dependent gene silencing can be achieved through whole-organism irradiation or the targeted illumination of specific cell populations. (cdc.gov)
We envisioned that enzyme-activatable cMOs could overcome these limitations, as the triggering enzymes could be expressed in individual cell populations using cis regulatory elements. (cdc.gov)

Here we performed a systematic analysis of two different induced pluripotent stem cell lines (iPSC 3.4 and 4.1) and an embryonic stem cell (ESC) line-derived cardiac myocytes at two different developmental stages. (nih.gov)
The same researchers later published another article with Shinya Yamanaka as co-author, where they again used laminin-211 for cardiac differentiation of human iPSC (Hirata, 2014). (biolamina.com)
Dr. Joseph Wu´s lab also showed that laminin-521 is an optimal matrix for chemically defined differentiation of human iPSC to cardiomyocytes (Burridge, 2014). (biolamina.com)
Biolaminin 521 is an optimal matrix for chemically defined differentiation of human iPSC to cardiomyocytes. (biolamina.com)

Reversible alteration of calcium dynamics in cardiomyocytes during acute hypoxia transient in a microfluidic platform. (unipd.it)
More interestingly, the protective actions of lncRNA GAS5 silence on hypoxia-stimulated cells were attenuated by miR-142-5p suppression. (molcells.org)
Collectively, this study demonstrated that silence of lncRNA GAS5 protected H9c2 cells against hypoxia-induced injury possibly via sponging miR-142-5p , functionally releasing TP53INP1 mRNA transcripts that are normally targeted by miR-142-5p . (molcells.org)
The expression of circ-JA760602 in AC16 cardiomyocytes subjected to hypoxia was measured by quantitative real-time polymerase chain reaction (qRT-PCR). (ehu.eus)

With the use of cardiac muscle-specific laminin-211, human iPSCs were effectively differentiated into cardiomyocytes using small molecules (Minami, 2012). (biolamina.com)
iPSCs were efficiently expanded and differentiated to cardiomyocytes on laminin-521 (Kuroda, 2015). (biolamina.com)

It was established that the addition of DMSO from the 5th to the 9th day of cultivation increased the efficiency of cardiomyocytes differentiation by one and a half times. (edu.ua)
In both cell maintenance and differentiation, EpiMOGRIFY defined conditions performed as well or better in all cases when compared to existing undefined conditions, significantly increasing cell growth and survival, as well as resulting in a higher differentiation efficiency. (technologynetworks.com)

Both ES cells and iPS cells are pluripotent stem cells with capabilities of indefinite self-renewal and can be differentiated into almost all cell types of the body, which make them valuable for studying early developmental biology, for modeling and as therapy for human diseases. (biomedcentral.com)
In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)
The related coactivator complexes SAGA and ATAC control embryonic stem cell self-renewal through acetyltransferase-independent mechanisms. (axonmedchem.com)

DNA methylation is considered among the most prominent epigenetic mechanisms in controlling the functions of genes related to cell differentiation, cell cycle, cell survival, autophagy, and embryo development. (ehu.eus)
Using single-cell RNA-sequencing data, we examined the expression profiles of Sox genes in the mouse incisor dental pulp. (ehu.eus)
In addition, Sox family genes colocalized with Runx2 and Lef1 , which are highly enriched in MSCs undergoing osteoblast differentiation. (ehu.eus)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)

We investigated the effect of 5-azacytidine (5-aza) on P19 cells to differentiate into a high-efficacy cardiomyocytes. (korea.ac.kr)
Human induced pluripotent stem (iPS) cells can differentiate into somatic cells ( 1 ). (spandidos-publications.com)
A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)

15 d) during cardiac differentiation in a chemically defined medium. (biolamina.com)
We started by developing chemically defined culture conditions in which the activities of specific growth factors could be identified and studied in a controlled manner. (qscience.com)
EpiMOGRIFY is an extension of the Company's proprietary direct cellular conversion technology, MOGRIFY®, and enables the identification of the optimal culture conditions required to maintain cells in chemically defined media. (technologynetworks.com)

The company has secured about $60M to date and developed a proprietary pro-regenerative protein library supported by a computationally driven drug discovery and development platform that enables them to characterize human stem cell-secreted proteins for therapeutic potential. (juvenatherapeutics.com)
Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells Biophysical Journal. (nottingham.ac.uk)
Specifically, human iPS cells could be utilized to generate patient-specific lineages for a variety of translational research. (biomedcentral.com)
Human embryonic stem cells also exhibit the Warburg effect ( 15 ). (spandidos-publications.com)
Electrical stimulation of human embryonic stem cells: Cardiac differentiation and the generation of reactive oxygen species. (unipd.it)
Laminin-211 has also been used for the culture of adult human cardiomyocytes (Kuroda, 2015). (biolamina.com)
This in accordance with recent data presented by researchers at Icagen Inc., demonstrating that the laminin-521 cell culture matrix maintains the differentiation potential of mouse and human satellite cell-derived myoblasts, even during long-term culture expansion (Penton, 2016). (biolamina.com)
Laminin-521 supports increased differentiation potential without altering the traditional Pax7/MyoD paradigm and the results are translational across several mouse backgrounds, human cells, and disease states. (biolamina.com)
Amend N, Thiermann H, Worek F, Wille T ( 2019 ) The arrhythmogenic potential of nerve agents and a cardiac safety profile of antidotes - A proof-of-concept study using human induced pluripotent stem cells derived cardiomyocytes (hiPSC-CM). Toxicol Lett 308:1-6. (multichannelsystems.com)
Induces the differentiation of human PSC-derived alveolar epithelial type II (AETII) to AETI cells (Ghaedi et al. (stemcell.com)
Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
These results show that for the first time, human endometrial stem cells can be differentiated into Schwann cells in 2D and 3D culture. (scirp.org)
Chemical conversion of human conventional Pluripotent Stem Cells to Trophoblast Stem Cells. (axonmedchem.com)
Stability of Imprinting and Differentiation Capacity in Naïve Human Cells Induced by Chemical Inhibition of CDK8 and CDK19. (axonmedchem.com)
Upon return in The Netherlands he worked with Christine Mummery and Hans Clevers on both human Embryonic as well as human Cardiac stem cells. (umcutrecht.nl)
PBK model allows the prediction of dose-response curves for implantation rat whole-embryo culture test, the rat limb bud human developmental toxicity. (cdc.gov)
This approach could therefore micromass test, and the embryonic stem cell test (EST) provide a means to reduce the need for animal testing in human risk assessment practices. (cdc.gov)
The possible donor-host rejection of human ES cells is another concern 3 . (bvsalud.org)
To date, four types of human dental stem cells have been isolated and characterized: (i) dental pulp stem cells (DPSCs) 12 (ii) stem cells from exfoliated deciduous teeth (SHED) 21 (iii) stem cells from apical papilla (SCAP) 31 (iv) periodontal ligament stem cells (PDLSCs) 28 . (bvsalud.org)

Laminin-521 supports increased proliferation during expansion and superior differentiation with myotube hypertrophy, larger myotubes and higher amounts of nuclei per myotube. (biolamina.com)

Unguided differentiation confirms HiPSC ability to generate cell derivatives of tissues arising from the three embryonic layers. (cellapplications.com)

Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
Membrane vesicles, actively released by cells, represent a mechanism of intercellular communication that is conserved evolutionarily and involves the transfer of molecules able to induce epigenetic changes in recipient cells. (unicyte.ch)
The practice of dentistry is likely to be revolutionized by biological therapies based on growth and differentiation factors that accelerate and/or induce a natural biological regeneration. (bvsalud.org)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)

For instance, in mouse embryos this occurs as early as day 6.5 post fertilization and involves the development of a population of cardiac precursor cells in the lateral posterior epiblast just prior to formation of the primitive streak - a structure which marks the beginning of gastrulation. (mechanobio.info)
Efficient generation of embryonic stem cells from single blastomeres of cryopreserved mouse embryos in the presence of signalling modulators. (axonmedchem.com)

Mitochondria not only serve as power plants in cells but also act as crucial regulators in many biological processes, including reactive oxygen species (ROS) signaling, redox balance, calcium homeostasis, protein quality control, and programmed cell death ( 3 , 4 ). (frontiersin.org)

In this review, we describe the cardiac differentiation from ES cells, iPS cells, and the current progress of using iPS cell-derived cardiomyocytes for heart disease modeling and for the development of therapeutic strategies. (biomedcentral.com)
Even so a electric battery of pitfalls restricts using this cell series in therapeutic program namely ethical problems involving devastation of embryo challenging isolation methods as well as the tendency to create tumours [6]. (bibf1120.com)
The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)

Estrogens are female sex hormones that have genotoxic and proliferation-enhancing effects in cells. (helsinki.fi)

Our research focuses on understanding how pluripotent mammalian stem cells maintain their undifferentiated state and undergo differentiation in culture - this reflecting my enduring interest in the emergence of diversity during mouse gastrulation. (qscience.com)
In addition to intracellular mitochondrial movement, intercellular mitochondrial transfer between mammalian cells has recently been discovered. (biomedcentral.com)

Optimal term of DMSO application into the culture was determined. (edu.ua)

Cardiac specification occurs very early on during embryonic development. (mechanobio.info)
During development, the nuclear translocation of β-catenin, a transcriptional cofactor in the Wnt signaling pathway, is controlled by the extent of cell-cell contacts formed between cells. (mechanobio.info)
DNA methyl transferases (Dnmts) control DNA methylation, the levels of which are differentially altered during embryonic development, and may determine cell differentiation fate as in the case of pluripotent inner cell mass (ICM) or trophectoderm (TE). (ehu.eus)
SOX transcription factors play key roles in cell differentiation and cell fate determination during development. (ehu.eus)
Schwann cells (SCs) have important roles in development, myelination and regeneration in the peripheral nervous system. (scirp.org)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
This can be applied in cGMP manufacture and enhances directed differentiation or cell conversion to support the development of scalable off-the-shelf therapies for diseases with a high unmet clinical need. (technologynetworks.com)
Owen Rackham, PhD, Assistant Professor at Duke-NUS Medical School, Singapore, and Co-founder and CTO, Mogrify, said: "Building on our development of the Mogrify technology to identify transcriptomic switches for cell conversion, this new technology published in Cell Systems adds an important layer of epigenetic specificity in our continuous search to drive cell identity with an increasing level of control. (technologynetworks.com)
This represents a unique opportunity to transform the development of existing cell therapies and will become instrumental in our bid to engineer scalable off-the-shelf therapies for diseases with a high unmet clinical need. (technologynetworks.com)
A tight spatial and timing regulation of growth factor action during embryonic development has been suggested [4]. (unicyte.ch)
This review considers the genetic and epigenetic control of nephrogenesis, together with the epigenetic mechanisms that accompany kidney development and recent advances in induced reprogramming and kidney cell regeneration in the context of DN. (emjreviews.com)
Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
The major areas of research that might have application in the development of regenerative endodontic techniques are (a) postnatal stem cells, (b) scaffold materials, (c) morphogen/growth factors, (d) implantation. (bvsalud.org)

Cardiovascular diseases often cause substantial heart damage and even heart failure due to the limited regenerative capacity of adult cardiomyocytes. (thno.org)

Embryonic stem (ES) cells are naturally derived from early stage embryos and induced pluripotent stem (iPS) cells are reprogrammed from somatic cells with overexpression of four reprogramming factors, Oct4, Sox2, Klf4 and c-Myc. (biomedcentral.com)

Because of this stem cell therapy provides emerged alternatively choice with potential benefits for sufferers with end-stage cardiovascular disease. (bibf1120.com)

Tahrir FG, Gordon J, Feldman AM, Cheung J, Khalili K, Mohseni Ahooyi T ( 2019 ) Evidence for the impact of BAG3 on electrophysiological activity of primary culture of neonatal cardiomyocytes. (multichannelsystems.com)

It is also extensively used as an extractant in biochemistry and cell biology. (wikipedia.org)
I cover mainly cell biology and physiology. (nottingham.ac.uk)
4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
Combining cell biology and electrophysiology, his work has the potential to create personalized disease models for future research. (lu.se)

This starts up an avenue for using adult stem cells in dealing with cardiovascular diseases. (bibf1120.com)
The most valuable cells for regenerative endodontics are postnatal or adult stem cells. (bvsalud.org)
Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)

After two days of culture in HSM, the expression levels of α-fetoprotein (AFP) and albumin, which are hepatocyte markers, were upregulated, suggesting that hepatocyte differentiation was initiated ( 19 ). (spandidos-publications.com)
Using immunocytochemistry, we show that differentiated cells express S100 and P75 markers. (scirp.org)
To determine which cells are expressing specific cellular markers and how these cells are organized relative to each other requires volumetric imaging of the pre-implantation embryos by confocal microscopy. (zeiss.com)

However, the engineering of more complex tissues consisting of large 3D structures remains a critical challenge because the penetration of oxygen, which is required for cell survival, is limited by diffusion to a distance of approximately 150 to 200 mm from the nearest blood vessel. (hindawi.com)
It was suggested that lactate promoted the survival of iPS cells cultured in a medium without glucose and supplemented with galactose. (spandidos-publications.com)
Glucose is essential for cell survival and is metabolized to pyruvate ( 13 ). (spandidos-publications.com)
Intercellular mitochondrial transfer can be regarded as an extension of intracellular mitochondrial movement or intercellular communication, which undoubtedly increases the mtDNA content of the recipient cells and restores the respiration and survival of the recipient cells. (biomedcentral.com)

Molecular Cell , 82 (1), 190-208. (axonmedchem.com)
Molecular cell, 82(1), 106-122. (axonmedchem.com)

This process involves the ability of cells to change pheno- type depending upon specific signals. (unicyte.ch)

These studies led to our discovery of a novel type of pluripotent epiblast stem cell (EpiSC) from the late epiblast layer of mouse and rat embryos. (qscience.com)

Individual exfoliated deciduous teeth (SHED) and adipose stem cells (ASC) were suggested seeing that substitute cell choice for cardiac regeneration. (bibf1120.com)
Center transplant and operative involvement can prolong the life span of an individual but they usually do not address the essential issue which may be the substitute or regeneration of cardiomyocytes [1-3]. (bibf1120.com)
There are limited numbers of publications about ES cells in pulp regeneration, due to the restricted policies regarding ES cell research over the past few years. (bvsalud.org)

Anatomy63

Organisms19

Diseases10

Chemicals and Drugs74

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes62

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science3

Vitro13

Endothelial cells10

Fibroblasts8

Lineage4

Pluripotency5

Embryoid5

Mesenchymal3

Promotes2

Fibroblast2

Neuronal1

ESCs2

Progenitors4

Tissue21

Characterization2

Assay4

Neural2

Induction7

Microenvironment3

Extracellular6

Vivo1

Apoptosis4

Populations5

IPSC4

Hypoxia4

IPSCs2

Efficiency2

Self-renewal3

Genes5

Differentiate3

Chemically defined3

Human19

Hypertrophy1

Generate1

Induce3

Pathways2

Mouse embryos2

Reactive oxygen1

Therapeutic3

Proliferation1

Mammalian2

Optimal1

Development13

Regenerative capacity1

Somatic cells1

Cardiovascular disease1

Neonatal1

Biology4

Adult stem3

Markers3

Survival4

Molecular2

Involves1

Embryos1

Regeneration3