• 2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
  • Equine mesenchymal stem cells from bonemarrow, adipose tissue and umbilical cord:immunophenotypic characterization anddifferentiation potential. (sciendo.com)
  • Bovine fetal mesenchymal stem cells exert antiproliferative efect against mastitis causing pathogen Staphylococcus aureus. (sciendo.com)
  • Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stem Cells from Sheep: Culture Characteristics. (sciendo.com)
  • Cryopreserved allogeneic mesenchymal stem cells enhance wound repair in full thickness skin wound model and cattle clinical teat injuries. (sciendo.com)
  • Poly(ADP-ribose): a signalling molecule in mesenchymal stem cells? (remedicon.hu)
  • These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)
  • Of particular interest to Dr. Ardehali is the intrinsic signaling that trigger cardiac regeneration early in life, molecular events that regulate developmental decisions instructing cardiac progenitor cells to adopt a specific cell fate, and delivery approaches of cardiovascular progenitors into an injured heart. (ucla.edu)
  • Chemical compounds that modulate atrial and ventricular cell fate could be used to improve subtype-specific differentiation of endogenous or exogenously delivered progenitor cells in order to promote cardiac regeneration. (biomedcentral.com)
  • Collectively, these results indicate the potential for therapeutic alteration of cell fate decisions and pathological gene regulatory networks by GATA4-targeted compounds modulating chamber-specific transcriptional programs in multipotent cardiac progenitor cells and cardiomyocytes. (biomedcentral.com)
  • Thus, small molecules capable of influencing the fate decisions and differentiation programs of multipotent progenitor cells could facilitate therapeutic regeneration of lost myocardium. (biomedcentral.com)
  • Recent Accomplishments - Starting in 2013, we incorporated Fluidigm-based multiplex single cell PCR equipment HD Biomark to analyze the differentiation of single cardiac progenitor cells in vitro and found that Nkx2.5 expression marks a subpopulation of committed endocardial precursor cells in the mouse heart (Li et al, Development 2015). (stanford.edu)
  • R4 RGS proteins suppress engraftment of human hematopoietic stem/progenitor cells by modulating SDF-1/CXCR4 signaling. (edu.hk)
  • This protocol introduces how to generate heart organoids from mouse embryonic stem cell-derived embryoid bodies (EB) in the presence of the laminin-entactin (LN/ET) complex and fibroblast growth factor 4 (FGF4) by three consecutive steps, 1) the culture of ESCs, 2) in vitro differentiation of ESCs into EBs and 3) in vitro culture of EBs for the generation of heart organoids. (researchsquare.com)
  • selectively promotes the differentiation of ESCs to cardiomyocytes in vitro between days 3-5. (tocris.com)
  • Background Embryonic stem cells (ESCs) can proliferate endlessly and are capable to differentiate into every cell lineages that make up the mature organism. (bioinbrief.com)
  • Extremely, nevertheless, our outcomes confirmed that the bioreactor differentiated ESCs maintained their capability to CL 316243 disodium salt manufacture exhibit pluripotency indicators, to type ESC-like colonies, and to generate teratomas upon transplantation, whereas the cells differentiated in adherent lifestyle dropped these features. (bioinbrief.com)
  • History Embryonic control cells (ESCs) are made from the internal cell mass (ICM) of pre-implantation embryos [1]. (bioinbrief.com)
  • These ESCs have the ability to remain undifferentiated and proliferate indefinitely in vitro , while maintaining the potential to differentiate into all three embryonic germ layers [1,2]. (bioinbrief.com)
  • This differentiation capacity makes ESCs an attractive cell source for cell/tissue alternative therapies for the treatment of human degenerative diseases. (bioinbrief.com)
  • The in vitro differentiation of ESCs into cardiomyocytes provides an opportunity to study the developmental aspects of cardiomyogenesis. (bioinbrief.com)
  • Presently, most protocols make use of stationary lifestyle to differentiate ESCs into cardiomyocytes [5-12]. (bioinbrief.com)
  • For 3D culture methods using embryonic stem cells (ESCs) or adult stem cells, ECMs including Matrigel and laminin were widely used for in vitro organogenesis,including the development of the brain 1 , stomach 2 , kidney 3 , and intestinal tissues 4 . (researchsquare.com)
  • Prof. Poon's research focuses on the application of human pluripotent stem cell-derived cardiomyocytes for disease modelling and drug screening. (edu.hk)
  • Immaturity of human pluripotent stem cell-derived cardiomyocytes has long been a barrier to the use of these cells for research and therapy. (edu.hk)
  • 2021). Remdesivir induces persistent mitochondrial and structural damage in human induced pluripotent stem cell derived cardiomyocytes. (edu.hk)
  • Importance of evaluating protein glycosylation in pluripotent stem cell-derived cardiomyocytes for research and clinical applications. (edu.hk)
  • Cell surface markers for immunophenotyping human pluripotent stem cell-derived cardiomyocytes. (edu.hk)
  • Human-induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have shed great light on cardiac regenerative medicine and specifically myocardial repair in heart failure patients. (biomedcentral.com)
  • Given the tumorgenic nature of SiPS, their predifferentiation into cardiomyocytes would be important for tumor-free cardiogenesis in the heart. (elsevierpure.com)
  • We describe cardiogenesis in vitro by differentiating EC and ES cells by way of embryo-like aggregates (embryoid bodies) into spontaneously beating cardiomyocytes. (uni-luebeck.de)
  • For the understanding of the spatiotemporal regulation of cardiogenesis, it is important to generate in vitro model of hearts. (researchsquare.com)
  • Many researchers have developed an in vitro system that mimics in vivo cardiogenesis in order to better understand the function of heart, and provide technical advantages for drug testing and model for cardiac pathology. (researchsquare.com)
  • The most widely accepted method for determining whether a population of cells is truly pluripotent is the teratoma assay, that is, verifying in vivo a cell population's ability to differentiate into each of the three germ layers via teratoma formation in mice. (genengnews.com)
  • These antibodies are added to live, unfixed cells in a single-step assay that takes only 30 minutes and has no adverse effects on stemness or proliferation. (genengnews.com)
  • Despite its broad use as the most common assay for assessing the pluripotency of stem cells, the teratoma assay has limitations. (genengnews.com)
  • The teratoma assay is also inherently variable, depending on the site of cell inoculation and the mouse strain used. (genengnews.com)
  • Another assay used to verify functional pluripotency is in vitro embryoid body formation. (genengnews.com)
  • Transcription factor GATA4-targeted compounds that have previously shown in vivo efficacy in cardiac injury models were tested for stage-specific activation of atrial and ventricular reporter genes in differentiating pluripotent stem cells using a dual reporter assay. (biomedcentral.com)
  • showed that the differentiation assay of benchmark dose. (cdc.gov)
  • Does not induce differentiation of vascular smooth muscle cells or endothelial cells. (tocris.com)
  • First, we cocultured dermal fibroblasts with dermal microvascular endothelial cells at a ratio of 2 : 1. (hindawi.com)
  • A fibrous sheet was formed by the interactions between the fibroblasts and the endothelial cells, and capillary-like structures were observed after 20 days of coculture. (hindawi.com)
  • ELISA demonstrated that vascularization was promoted by significant upregulation of vascularization associated growth factors due to interactions among the 3 types of cells in the bilayer, as compared to cocultures of fibroblast and endothelial cells and monocultures. (hindawi.com)
  • Most of these methods involve culturing endothelial cells on or within ECM substrates (e.g., collagen, fibrin, fibronectin, laminin, etc.) or other types of biomaterials to form capillary-like structures [ 15 ]. (hindawi.com)
  • In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
  • Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
  • Mammalian heart development involves differentiation of diverse cell types, including atrial and ventricular muscle cells known as cardiomyocytes (CMs), specific myocytes constituting conducting tissue such as Purkinje fibers, smooth muscle (SM) cells, and non-muscle cells including endothelial cells (ECs) and neuronal cells. (researchsquare.com)
  • Methylation state on the histone tail on the chromatin, histone code, in endothelial cells was read via hi-speed deep sequencer. (medicalxpress.com)
  • Dynamically and epigenetically coordinated gata/ets/sox transcription factor expression is indispensable for endothelial cell differentiation. (medicalxpress.com)
  • During the creation of new blood vessels, the vascular endothelial growth factor (VEGF) protein differentiates stem cells into vascular endothelial cells and stimulates them to create new blood vessels. (medicalxpress.com)
  • The protein GATA2, which binds to ETV2 and supports vascular endothelial differentiation, was induced immediately thereafter. (medicalxpress.com)
  • Transcription factors SOX and FLI1, both important for endothelial differentiation, were induced between 12 and 24 hours. (medicalxpress.com)
  • At 48 hours, after differentiation into vascular endothelium was determined, a system of transcription was established in which genes unique to vascular endothelial differentiation were induced. (medicalxpress.com)
  • The surgical procedure for delivery of embryonic stem cell-derived endothelial cells to the ischemic hindlimb is demonstrated, with non-invasive tracking by bioluminescence imaging. (jove.com)
  • We were interested in the developmental potential of single cardiac progenitor cell into cardiomyocytes, smooth muscle cells, and endothelial cells. (stanford.edu)
  • What makes characterization challenging is that heterogeneity within a given stem cell population can cause variability in the differentiation into important downstream lineages, such as cardiomyocytes and hepatocytes. (genengnews.com)
  • Cell Characterization. (cellapplications.com)
  • To account for this high level of functional variation, verifying the pluripotency of stem cells-from the generation of a new cell line through its expansion for an experiment-is crucial. (genengnews.com)
  • of functional cardiomyocytes fully. (bioinbrief.com)
  • These functional characteristics are in accordance with the expression of α- and β-cardiac myosin heavy chain at early differentiation stages and the additional expression of ventricular-specific MLC-2V and atrial-specific ANF genes at terminal stages demonstrated by reverse transcription polymerase chain reaction (RT-PCR) analysis. (uni-luebeck.de)
  • iPSCs have great replicative ability and demonstrated potential to form functional cardiomyocytes (CMs). (creative-bioarray.com)
  • We deliver ready-to-use, functional cardiomyocytes that are desirable in vitro models for high content toxicity and drug screening, and cell regeneration research. (creative-bioarray.com)
  • Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)
  • Generation of functional heart organoids from mouse embryonic stem cells. (researchsquare.com)
  • The generated heart organoids possess structural and functional capacity similar to atrial and ventricular parts of in vivo embryonic heart. (researchsquare.com)
  • In the present review we focus on the extracellular vesicle-induced epigenetic changes in recipient cells that may lead to phenotypic and functional modifications. (unicyte.ch)
  • Information exchange between cells coordinates devel- opment and functional interplay in complex organisms. (unicyte.ch)
  • Furthermore, it is unknown whether endogenous progenitors/stromal cells in the adult human heart could be chemically induced to generate functional atrial or ventricular heart muscle to treat adult heart diseases. (biomedcentral.com)
  • Animals injected with cell-free solution (control group) demonstrated significant left ventricular (LV) functional deterioration, whereas grafting of iPSC-CMs attenuated this remodeling process. (biomedcentral.com)
  • Myocardial infarction (MI), the most common heart disease, is characterized by a significant reduction in the number of functional cardiomyocytes and leads to the development of progressive heart failure. (biomedcentral.com)
  • The greatest goal in cardiac regenerative medicine is normally to make in large-scale, filtered cardiomyocytes which are ideal for cell transplantation highly. (bioinbrief.com)
  • Such cell transplantation therapies would need the effective seeding of as many as 1 108 donor cardiomyocytes per individual [4]. (bioinbrief.com)
  • Objective: To reprogram SMs to pluripotency and show that reprogrammed SMs (SiPS) express embryonic gene and microRNA profiles and that transplantation of predifferentiated cardiac progenitors reduce tumor formation. (elsevierpure.com)
  • In vivo studies in an experimental model of acute myocardial infarction showed extensive survival of SiPS and SiPS-derived cardiomyocytes in mouse heart after transplantation. (elsevierpure.com)
  • The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
  • In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
  • Cell labeling, tracking, and molecular biology techniques indicated that the grafted cardiomyocytes survived in the rat heart 1 month after iPSC-CM transplantation. (biomedcentral.com)
  • Heart regenerative therapy, such as transplantation of myocytes [ 4 ] and cardiomyocytes to replace lost cells, has become a new strategy for the treatment of heart failure caused by MI [ 5 ]. (biomedcentral.com)
  • Our data shows that in contrast to static tradition, ESC ethnicities caused to differentiate toward cardiomyocytes in suspension bioreactors retain some capability to exhibit pluripotency indicators, to type. (bioinbrief.com)
  • We explore the recently described metabolic surge event that occurs as pluripotency is lost and stem cells commit to differentiate. (conditionmed.org)
  • Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
  • They found that the histone code, which alters the transcriptional state of the gene, changes over time as stem cells differentiate into blood vessels in response to a stimulus. (medicalxpress.com)
  • HiPSC differentiate into cell derivatives from the 3 embryonic layers: Neuronal marker beta III tubulin (TUJ1), Smooth Muscle Actin (SMA) and Hepatocyte Nuclear Factor 3 Beta (HNF3b). (cellapplications.com)
  • We are engineering embryonic stem-like cells to autonomously differentiate into cardiomyocytes, recognize and bind to damaged heart cells and then activate a defined program. (regensci.org)
  • We will differentiate P19 cells using BMP4 and transcription factor Nkx2.5 and then use G418 resistance ( http://www.invivogen.com/family.php?ID=6 ) to select for those cells that differentiated into cardiomyocytes. (regensci.org)
  • They have the capacity for unlimited proliferation in vitro while maintaining the potential to differentiate into derivatives of the three germ layers. (biomedcentral.com)
  • A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)
  • Induces the differentiation of human PSC-derived alveolar epithelial type II (AETII) to AETI cells (Ghaedi et al. (stemcell.com)
  • The developing embryo, from which pluripotent stem cells originate, undergoes a series of dynamic metabolic transitions synchronized to its molecular development. (conditionmed.org)
  • Molecular endpoints indicate a role for reactive oxygen species and changes in cell membrane proteins. (nottingham.ac.uk)
  • Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
  • However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
  • Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells Biophysical Journal. (nottingham.ac.uk)
  • She then received a prestigious Commonwealth Scholarship for her DPhil research in the University of Oxford to study the molecular mechanisms of Duchenne Muscular Dystrophy, and was also a recipient of the American Heart Association Postdoctoral Fellowship in the University of California, San Francisco where she first started her training in pluripotent stem cell technology. (edu.hk)
  • Molecular Cell , 82 (1), 190-208. (axonmedchem.com)
  • Molecular cell, 82(1), 106-122. (axonmedchem.com)
  • Epithelial cells were then seeded on the fibrous sheet to assemble the bilayered tissue. (hindawi.com)
  • Comparison of NOD-like receptors in limbal stem cell-derived corneal epithelial cells and in immortalized corneal epithelial cell line. (remedicon.hu)
  • This kit, used in combination with the analysis of colony morphology, promotes the selection of high-quality, undifferentiated stem cell colonies, thereby reducing wasted time. (genengnews.com)
  • Previously, in the region that controls the function of the transcription factor that promotes differentiation from ES cells to a specific cell type, bivalent modifications of histones such as the accelerator and brake histone marks for transcription were thought to have coexisted. (medicalxpress.com)
  • Promotes self-renewal and maintains pluripotency of human embryonic stem cells and mouse Epi-stem cells when used in combination with CHIR99021 (Kim et al. (stemcell.com)
  • The platform utilizes data from more than 100 human cell/tissue types (available via the ENCODE and Epigenome Roadmap consortia) to accurately define culture conditions that can maintain the cell identity or induce cell conversion. (technologynetworks.com)
  • International bi-monthly journal of cell signaling, tissue protection, and translational research. (conditionmed.org)
  • The blastocyst comprises the inner cell mass (ICM), which gives rise to the three primary germ layers and consequently the fetus, and the trophectoderm (TE), which gives rise to the extraembryonic and placental tissue. (conditionmed.org)
  • Thus, the long-term survival and function of 3D tissues depend on the rapid development of new blood vessels to provide nutrients and oxygen to cells in the center of the tissue grafts. (hindawi.com)
  • Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
  • I returned full time to Imperial College in 1999 to take up a lectureship in cell biology and tissue engineering and was also actively involved in establishing the Tissue Engineering Centre. (nottingham.ac.uk)
  • I was also affiliated with the Wolfson Centre for Stem Cells, Tissue Engineering and Modelling (STEM), part of the Centre for Biomolecular Sciences, which is now the Biodiscovery Institute. (nottingham.ac.uk)
  • My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. (nottingham.ac.uk)
  • Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
  • For this purpose, we use human stem cells that play a pivotal role in tissue development, maintenance and function. (gsi.de)
  • The relevance of these phenomena in stem cell biology and tissue repair is discussed. (unicyte.ch)
  • First, they used human pluripotent stem cells to develop 3D embryoid bodies to mimic early steps in tissue and organ formation in humans. (allmymedicine.com)
  • Since Alpha-MHC is expressed in differentiated cardiac tissue, we will use antibiotic resistance to select only the differentiated cardiomyocytes. (regensci.org)
  • The key elements of tissue engineering are stem cells, morphogen, and a scaffold of extracellular matrix. (bvsalud.org)
  • Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)
  • For testing the influence of exogenous compounds on cardiac differentiation the teratogenic compound retinoic acid (RA) was applied during distinct stages of embryoid body development. (uni-luebeck.de)
  • A temporally controlled influence of RA on cardiac differentiation and expression of cardiac-specific genes was found. (uni-luebeck.de)
  • Electrical stimulation of human embryonic stem cells: Cardiac differentiation and the generation of reactive oxygen species. (unipd.it)
  • Pluripotent stem cells have great potential for use in regenerative medicine and are an important tool for understanding developmental and disease mechanisms. (genengnews.com)
  • During cardiomyocyte differentiation three distinct developmental stages were defined by expression of specific action potentials and ionic currents measured by the whole-cell patch-clamp technique. (uni-luebeck.de)
  • Here we performed a systematic analysis of two different induced pluripotent stem cell lines (iPSC 3.4 and 4.1) and an embryonic stem cell (ESC) line-derived cardiac myocytes at two different developmental stages. (nih.gov)
  • Pharmacological modulation of cell fate decisions and developmental gene regulatory networks holds promise for the treatment of heart failure. (biomedcentral.com)
  • We subsequently became interested in developing a developing heart expression atlas in order to identify the cell type, developmental stage, and anatomical location of each single cardiac cells during mouse heart development using a random forest-based computational algorithm that we termed ATLAS-seq. (stanford.edu)
  • for developmental toxicity by combining in vitro toxicity data and calculated an even higher number of laboratory animals in silico kinetic modeling. (cdc.gov)
  • response curves for rat were found to be in concordance with the 2004), in vitro and in silico alternatives for developmental embryotoxic dose levels measured in reported in vivo rat studies. (cdc.gov)
  • Whereas early differentiated cardiomyocytes are characterized by action potentials and ionic currents typical for early pacemaker cells, terminally differentiated cardiomyocytes show action potentials and ionic currents inherent to ventricular-, atrial- or sinus nodal-like cells. (uni-luebeck.de)
  • Due to cellular heterogeneity in the heart, the activation of gene programs representing specific atrial and ventricular cardiomyocyte subtypes would be highly desirable. (biomedcentral.com)
  • More detailed structure-function analysis revealed a distinct subclass of GATA4/NKX2-5 inhibitory compounds with an acetyl lysine-like domain that contributed to ventricular cells (%Myl2-eGFP+). (biomedcentral.com)
  • Cell replacement therapy also reversed ventricular remodeling, indicating the potential of iPSC-CMs for cardiac repair strategies. (biomedcentral.com)
  • Although a small percentage of the cells may be capable of proliferation [3], this CL 316243 disodium salt manufacture is usually not sufficient for regeneration after myocardial injury. (bioinbrief.com)
  • The practice of dentistry is likely to be revolutionized by biological therapies based on growth and differentiation factors that accelerate and/or induce a natural biological regeneration. (bvsalud.org)
  • There are limited numbers of publications about ES cells in pulp regeneration, due to the restricted policies regarding ES cell research over the past few years. (bvsalud.org)
  • EpiMOGRIFY-predicted factors are able to maintain astrocytes and cardiomyocytes in vitro in chemically defined media and promote the generation of astrocytes and cardiomyocytes from neural progenitors and embryonic stem cells, respectively. (technologynetworks.com)
  • and, using novel in vitro and in vivo models to identify pure populations of cardiovascular progenitors capable of engraftment into the host myocardium. (ucla.edu)
  • SSCs divide asymmetrically to either replenish their numbers (self-renewal) or produce undifferentiated progenitors that proliferate before committing to differentiation. (bvsalud.org)
  • Cardiomyocytes are terminally differentiated muscle mass cells in the adult mammalian heart, which do not divide. (bioinbrief.com)
  • Combining transcriptomic, proteomic and microRNA profiling, she revealed novel regulatory mechanisms that facilitate the generation of cardiomyocytes with adult-like mitochondrial and metabolic properties. (edu.hk)
  • Endogenous adult atem cells do not repair the heart, and the heart does not regenerate. (regensci.org)
  • There are a number of California institutions that have strong programs in adult and other stem cells, for example, that are just beginning to build embryonic stem cell capabilities. (ca.gov)
  • One way we did this was by converting adult fibroblasts, or skin cells, into interneurons. (lu.se)
  • Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
  • The most valuable cells for regenerative endodontics are postnatal or adult stem cells. (bvsalud.org)
  • Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)
  • However, the engineering of more complex tissues consisting of large 3D structures remains a critical challenge because the penetration of oxygen, which is required for cell survival, is limited by diffusion to a distance of approximately 150 to 200 mm from the nearest blood vessel. (hindawi.com)
  • Comparison of human mesenchymal stromal cells from four neonatal tissues: Amniotic membrane, chorionic membrane, placental decidua and umbilical cord. (sciendo.com)
  • Particularly, human embryonic stem cells (hES cells), which can give rise to all tissues of an organism (Figure A), are used to generate mature cells of the heart (Figure B) allowing us to study radiation/microgravity-induced cardiovascular alterations, which may occur during long-term space missions. (gsi.de)
  • Unguided differentiation confirms HiPSC ability to generate cell derivatives of tissues arising from the three embryonic layers. (cellapplications.com)
  • We also use human stem cells as a test-tube model to study the process of heart formation and to create new human heart tissues and organs from these stem cells. (stanford.edu)
  • Using models derived from human cells and tissues, called in vitro models, researchers designed a 3D organoid model that mimics how the human heart develops. (allmymedicine.com)
  • All tissues originate from stem cells. (bvsalud.org)
  • In both in vivo and in vitro experiments increased levels of glucose in myocardiomyctes led to significant upregulation of miR-1 and miR-206 with resulting modulation of HSP60 leading to accelerated glucose-mediated apoptosis in cardiomyocytes. (wikipedia.org)
  • Additionally, PSCs - both embryonic stem cells and induced pluripotent stem cells - provide insights into the mechanisms involved in the differentiation and specification of heart cells. (ucla.edu)
  • A well-established method of cardiomyocyte differentiation from PSCs provides an ideal source of cardiomyocytes and makes regenerative therapy for myocardial muscle damage a promising strategy. (biomedcentral.com)
  • 2018). Development of bovine embryos in vitro in coculture with murine mesenchymal stem clls and embryonic fibroblasts. (sciendo.com)
  • The GloLIVE™ Human Pluripotent Stem Cell Live Cell Imaging Kit and individual GloLIVE antibodies available from R&D Systems allow you to verify the pluripotency status of your stem cells by monitoring the expression of both positive and negative markers ( Figure 1 ). (genengnews.com)
  • The kit contains a set of azide-free, fluorochrome-conjugated GloLIVE antibodies that recognize two positive markers and one negative marker of human pluripotent stem cells. (genengnews.com)
  • Pluripotency of live human stem cell colonies was verified using a NorthernLights™ (NL) 493-conjugated Mouse Anti-Human SSEA-4 Monoclonal Antibody (green) and a NL557-conjugated Mouse Anti-Human TRA-1-60(R) Monoclonal Antibody (red). (genengnews.com)
  • Dr. Ardehali uses novel transgenic mouse models, as well as mostly human pluripotent stem cells (PSC) to address his research questions. (ucla.edu)
  • At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)
  • The company has secured about $60M to date and developed a proprietary pro-regenerative protein library supported by a computationally driven drug discovery and development platform that enables them to characterize human stem cell-secreted proteins for therapeutic potential. (juvenatherapeutics.com)
  • A) Colonies of human embryonic stem cells, bright field microscopy, scale bar: 100µm. (gsi.de)
  • B) Human cardiomyocytes immunostained for α-Actinin (red). (gsi.de)
  • D) Karyotype of a human cell after heavy ion exposure revealed by mFISH technique. (gsi.de)
  • We found that early exposure to human-relevant levels of cadmium lead to a dramatic inhibitory effect on cardiomyocyte differentiation, whereas later stage exposures did not have this effect," said Xian Wu, Ph.D., who conducted these studies. (allmymedicine.com)
  • Xian Wu et al, Cardiac Development in the Presence of Cadmium: An in Vitro Study Using Human Embryonic Stem Cells and Cardiac Organoids, Environmental Health Perspectives (2022). (allmymedicine.com)
  • Her current focus is to utilise her cardiomyocyte platform for the modelling of human cardiac diseases, investigations of cardiotoxicity and discovery of cardioprotective agents. (edu.hk)
  • Generation of genomic-integration-free human induced pluripotent stem cells and the derived cardiomyocytes of X-linked dilated cardiomyopathy from DMD gene mutation. (edu.hk)
  • Integrated transcriptomic and regulatory network analyses identify microRNA-200c as a novel repressor of human pluripotent stem cell-derived cardiomyocyte differentiation and maturation. (edu.hk)
  • The survival of grafted cardiomyocytes was confirmed by observing the fluorescent cell tracer Vybrant™ CM-DiI or expression of the enhanced green fluorescent protein (eGFP) in transplanted cells, or survival was demonstrated by polymerase chain reaction (PCR)-based detection of human mitochondrial DNA. (biomedcentral.com)
  • This approach could therefore micromass test, and the embryonic stem cell test (EST) provide a means to reduce the need for animal testing in human risk assessment practices. (cdc.gov)
  • As of today, California is the largest and most stable source of funding for human embryonic stem cell research in the world," Klein said. (ca.gov)
  • The Comprehensive Grants approved today will support mature, ongoing studies on human embryonic stem cells (hESCs) by scientists with a record of accomplishment in the field. (ca.gov)
  • These grants provide substantial support to a pool of very distinguished researchers in human embryonic stem cell research," declared Zach W. Hall, Ph.D., CIRM's President and Chief Scientific Officer. (ca.gov)
  • We focused our initial grants on human embryonic stem cells specifically," Klein said, "because human embryonic stem cell research receives minimal funding from the federal government, and even those funds are restricted to lines of questionable value. (ca.gov)
  • Development of new ways of deriving hESCs and investigating the special capabilities of newly-derived human cell lines. (ca.gov)
  • Chemical conversion of human conventional Pluripotent Stem Cells to Trophoblast Stem Cells. (axonmedchem.com)
  • Revealing cell populations catching the early stages of the human embryo development in naïve pluripotent stem cells. (axonmedchem.com)
  • Stability of Imprinting and Differentiation Capacity in Naïve Human Cells Induced by Chemical Inhibition of CDK8 and CDK19. (axonmedchem.com)
  • The possible donor-host rejection of human ES cells is another concern 3 . (bvsalud.org)
  • To date, four types of human dental stem cells have been isolated and characterized: (i) dental pulp stem cells (DPSCs) 12 (ii) stem cells from exfoliated deciduous teeth (SHED) 21 (iii) stem cells from apical papilla (SCAP) 31 (iv) periodontal ligament stem cells (PDLSCs) 28 . (bvsalud.org)
  • In addition, after transcription, a wide array of RNA-binding proteins interacts with cis -acting elements located mainly in the 3' untranslated region, determining the fate of mRNAs in eukaryotic cells. (frontiersin.org)
  • These include contractile activity (morphological scoring system), cell activity, total protein content, ROS production, DNA damage, transmembrane proteins expression (connexin43, integrin β1) and stem cell migration. (nottingham.ac.uk)
  • The cytoskeleton is a highly dynamic network of filamentous proteins that enables the active transport of cellular cargo, transduces force, and when assembled into higher-order structures, forms the basis for motile cellular structures that promote cell movement. (mechanobio.info)
  • Bound cells will express anti-apoptosis and anti-necrosis proteins to prevent cell death, express increased amounts of a cell-cell adhesion molecule to stimulate the formation of a sheet of cells and to cause some of the cells to penetrate into the inner layers of the heart. (regensci.org)
  • culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
  • An important aspect of ESC research focuses on elucidating the mechanisms of differentiation from the pluripotent ESC to numerous terminally differentiated cell types. (bioinbrief.com)
  • Moreover, full EBs of the iPS 4.1 cell line after oxidative stress induction by SNAP was protected at day-8 samples. (nih.gov)
  • Creative Bioarray provides top quality service for directed-differentiation of iPSCs into cardiomyocytes using our proprietary induction protocol and reagents. (creative-bioarray.com)
  • The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
  • Regenerative medicine has made remarkable progress due to research with embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. (medicalxpress.com)
  • The 29-member Independent Citizens Oversight Committee (ICOC), governing board of the California Institute for Regenerative Medicine (CIRM), today approved 29 Comprehensive Research Grants for approximately $74.6 million over four years, to accomplished stem cell investigators at academic and non-profit research centers throughout the state. (ca.gov)
  • Regenerative endodontic procedures can be defined as biologically based procedures designed to replace damaged structures, including dentin and root structures, as well as cells of the pulp-dentin complex. (bvsalud.org)
  • The major areas of research that might have application in the development of regenerative endodontic techniques are (a) postnatal stem cells, (b) scaffold materials, (c) morphogen/growth factors, (d) implantation. (bvsalud.org)
  • The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. (sciendo.com)
  • However, only miR-1-2 was involved in abnormal proliferation in EVI1 expressing cell lines. (wikipedia.org)
  • The ability to systematically identify the optimal culture conditions, closely mimicking the in vivo microenvironment, will enable scientists to convert and maintain any cell type more efficiently and with even greater accuracy. (technologynetworks.com)
  • The cell phenotype is therefore determined by signals that target the cells received within a defined microenvironment. (unicyte.ch)
  • In the earliest stages of development, the physical properties of the microenvironment can direct cell differentiation, and initiate the coordinated movement of groups of cells to establish the patterns that will define how the body is arranged. (mechanobio.info)
  • The expression of cardiac markers in iPSC-derived cardiomyocytes and the electrical properties of the cells. (creative-bioarray.com)
  • The platform models the epigenetic state of the cell to predict the switches important for cell identity, cell maintenance, directed differentiation and cell conversion. (technologynetworks.com)
  • Owen Rackham, PhD, Assistant Professor at Duke-NUS Medical School, Singapore, and Co-founder and CTO, Mogrify, said: "Building on our development of the Mogrify technology to identify transcriptomic switches for cell conversion, this new technology published in Cell Systems adds an important layer of epigenetic specificity in our continuous search to drive cell identity with an increasing level of control. (technologynetworks.com)
  • Membrane vesicles, actively released by cells, represent a mechanism of intercellular communication that is conserved evolutionarily and involves the transfer of molecules able to induce epigenetic changes in recipient cells. (unicyte.ch)
  • Similarly, reprogramming of somatic cells involves a complex interaction among intracellular and extracellular signals leading to epigenetic remodeling [6]. (unicyte.ch)
  • Using this type of direct conversion approach rather than making them from induced pluripotent stem cells, where they revert into a naïve state, helps to retain the epigenetic age of the patient in the generated interneurons. (lu.se)
  • ES cells are pluripotent cells, which mean that they can give rise to all differentiated cell types derived from all three germ layers. (bvsalud.org)
  • After analysis of full embryoid bodies (EBs) and cardiac marker (VCAM and cardiac troponin I) positive cells of three lines at 6 conditions (32 different conditions altogether), we found significant SI/R injury-induced cell death in both full EBs and VCAM+ cardiac cells at later stage of their differentiation. (nih.gov)
  • All of these observations suggest that mis-regulation of miR-1 and miR-133a can result in tumorogenesis via abolition of the suppressive effect they have on certain gene targets and of the removal of the promotion of differentiation of the cells exerted my miR-1. (wikipedia.org)
  • Together, our study reveals that CNOT3 - likely via the CCR4-NOT complex - actively degrades transcripts encoding differentiation factors to sustain the spermatogonial pool and ensure the progression of spermatogenesis, highlighting the importance of CCR4-NOT-mediated post-transcriptional gene regulation during male germ cell development. (bvsalud.org)
  • During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
  • Also, these heart organoids exhibited the ultrastructural, histochemical and gene expression profile similarities to embryonic hearts via consecutive morphological changes like self-organization and, therefore, without the requirement of complex differentiation protocols. (researchsquare.com)
  • A collaborative research group from Kumamoto University, Kyoto University, and the University of Tokyo in Japan investigated the changes in gene functions that occur when stem cells become vascular cells. (medicalxpress.com)
  • Hydrogel microspheres increase cell survival and increase new bone volume in a gene therapy bone formation model. (biomaterials.org)
  • In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)
  • The related coactivator complexes SAGA and ATAC control embryonic stem cell self-renewal through acetyltransferase-independent mechanisms. (axonmedchem.com)
  • The authors propose that miR-1 and miR-133a act to repress isoforms of genes that are not normally expressed in muscle cells. (wikipedia.org)
  • the EST, which assesses the effects of compounds on the differentiation of mouse embryonic stem cells into contracting cardiomyocytes, can be used to rank the potency of chemicals within a series of alkoxyacetic acid metabolites formed from The implementation of the European REACH (Registration, glycol ethers. (cdc.gov)
  • The miR-1 microRNA precursor is a small micro RNA that regulates its target protein's expression in the cell. (wikipedia.org)
  • Expression of miR-1 but not miR-133a gave transcriptional profiles that were consistent with a strong promyogenic influence on the cells, again demonstrating the role of miR-1 in muscle differentiation from precursor stem cells. (wikipedia.org)
  • Immunoselected STRO-3+ mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis. (sciendo.com)
  • For instance, in mouse embryos this occurs as early as day 6.5 post fertilization and involves the development of a population of cardiac precursor cells in the lateral posterior epiblast just prior to formation of the primitive streak - a structure which marks the beginning of gastrulation. (mechanobio.info)
  • Manually picking colonies that contain pluripotent stem cells is possible through an analysis of colony morphology. (genengnews.com)
  • Three weeks later, GFP colonies of SiPS were isolated and propagated in vitro. (elsevierpure.com)
  • Our HiPSCs display classic pluripotent stem cell morphology, with a high nucleus to cytoplasm size ratio, as well as they are amenable to be cultivated in serum-free media, independent of feeder cells and of feeder-conditioned media as colonies or high density monolayers. (cellapplications.com)
  • The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
  • MiR-1 has key roles in the development and differentiation of smooth and skeletal muscles. (wikipedia.org)
  • This can be applied in cGMP manufacture and enhances directed differentiation or cell conversion to support the development of scalable off-the-shelf therapies for diseases with a high unmet clinical need. (technologynetworks.com)
  • This represents a unique opportunity to transform the development of existing cell therapies and will become instrumental in our bid to engineer scalable off-the-shelf therapies for diseases with a high unmet clinical need. (technologynetworks.com)
  • Here, we consider the metabolism of the early embryo through development, and look at the nutrient milieu within the developing stem cell niche. (conditionmed.org)
  • 2020). This places metabolism at the forefront of development and cell state decisions. (conditionmed.org)
  • It is only through understanding embryonic metabolism and development that we can derive and maintain different in vitro stem cell states for disease modeling and therapies. (conditionmed.org)
  • We conclude that ES cell-derived cardiomyocytes provide an excellent cellular model to study early cardiac development and to perform pharmacological and embryotoxicological investigations. (uni-luebeck.de)
  • A tight spatial and timing regulation of growth factor action during embryonic development has been suggested [4]. (unicyte.ch)
  • Cardiac specification occurs very early on during embryonic development. (mechanobio.info)
  • During development, the nuclear translocation of β-catenin, a transcriptional cofactor in the Wnt signaling pathway, is controlled by the extent of cell-cell contacts formed between cells. (mechanobio.info)
  • Li et al, Development 2019) as well as the developing cardiac conduction system at embryonic day 16.5 of mouse development (Goodyer et al, Circulation Research 2019). (stanford.edu)
  • They then used a 2D in vitro model that included a fluorescent regulatory protein system (NKX2-5) known to be involved in heart development, which allowed them to look at cadmium toxicity after exposure. (allmymedicine.com)
  • Protocol Development Reveals Impact of Sample Preparation on the Accuracy of Identifying Cardiomyocytes by Flow Cytometry. (edu.hk)
  • In vitro toxicity data of these metabolites derived in the development of validated and accepted in vitro and in silico embryonic stem cell test were used as input in the PBK model to extrapolate in vitro concentration-response curves to predicted approaches is urgently needed. (cdc.gov)
  • Tumor initiation, development, and relapse may be closely associated with cancer stem cells (CSCs). (nature.com)
  • hES cells also serve as a basis for cerebral organoid models (Figure C) to better understand e.g. the mechanisms of cognitive impairment and radiation necrosis as severe side effects of radiation therapies. (gsi.de)
  • In vitro prevascularization can result in faster integration with the host vascular network [ 14 ]. (hindawi.com)
  • In the process of blood vessel differentiation, the function of the protein ETS variant 2 (ETV2), which determines the differentiation into vascular endothelium, was first induced within 6 hours of differentiation stimulation. (medicalxpress.com)
  • 2019). Akin to the dynamic nutrient requirements of the developing embryo, discrete in vitro cell states have distinct metabolic profiles (Zhou et al. (conditionmed.org)
  • In any case, the respective cell types may then be selectively cultured to enrich their percentage population to eventually obtain a single cell type and culture. (justia.com)
  • The Cell Surface Marker CD36 Selectively identifies Matured, Mitochondrial-rich hPSC-Cardiomyocytes. (edu.hk)