• The effect of reactive oxygen species on cardiomyocyte differentiation of pluripotent stem cells. (musc.edu)
  • Researchers at Michigan Tech and Harvard Medical School have developed a new way to create cardiomyocytes from induced pluripotent stem cells (iPSCs), for improved cardiac cell therapy. (medgadget.com)
  • Induced pluripotent stem cell-derived cardiomyocytes beating in substrate. (medgadget.com)
  • Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids. (tocris.com)
  • During the last several years, his lab has developed novel methods for multi-lineage differentiation of pluripotent stem cells for cell therapy in mice models of muscular dystrophies and injuries. (uh.edu)
  • Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. (rndsystems.com)
  • Co-differentiation and Co-maturation of Human Cardio-pulmonary Progenitors and Micro-Tissues from Human Induced Pluripotent Stem Cells. (rndsystems.com)
  • Recapitulating human cardio-pulmonary co-development using simultaneous multilineage differentiation of pluripotent stem cells. (rndsystems.com)
  • Replating Protocol for Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. (rndsystems.com)
  • By demonstrating the generation of cardiomyocytes from murine pluripotent stem cells, their concept shows promise as a regenerative therapy. (shinshu-u.ac.jp)
  • We had previously found that iSN04 promoted myogenic precursor cells (myoblasts) to differentiate into skeletal muscle cells and had hypothesized that the aptamer also enhanced differentiation of pluripotent stem cells. (shinshu-u.ac.jp)
  • However, when these pluripotent stem cells were treated after experiencing differentiating conditions for five days, specific marker genes were upregulated, and the cells committed to forming beating cardiomyocytes. (shinshu-u.ac.jp)
  • Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
  • To ensure researchers have success working with our human induced pluripotent stem cell lines in their lab, our Angel Nelson highlights nuanced techniques and helpful tips while demonstrating our cardiac differentiation protocol for the WTC parental line and our gene-edited cells. (alleninstitute.org)
  • Furthermore, we conduct a cross-species prediction of cardiomyocyte subtypes from in vitro-derived human induced pluripotent stem cells and unexpectedly uncover a predominance of left ventricular (LV) identity that we confirmed by an LV-specific TBX5 lineage tracing system. (stanford.edu)
  • selectively promotes the differentiation of ESCs to cardiomyocytes in vitro between days 3-5. (tocris.com)
  • Induces differentiation of cardiomyocytes from human ESCs and iPSCs. (rndsystems.com)
  • The team, led by Associate Professor Tomohide Takaya from the Department of Agricultural and Life Sciences at Shinshu University, recently discovered that an anti-nucleolin aptamer, myogenetic oligodeoxynucleotide iSN04, induced myocardial differentiation in embryonic stem cells (ESCs). (shinshu-u.ac.jp)
  • VE-CADHERIN is expressed transiently in early ISL1 + cardiovascular progenitor cells and facilitates cardiac differentiation. (nih.gov)
  • Interestingly, contradictory data exist for transformation of bone marrow derived progenitor cells (BMPC) into new cardiomyocytes and alternative mechanisms as enhanced neovascularization, enhanced scar tissue formation due to augmented inflammatory response and decreased apoptosis have been suggested for the benefical effects of these cells on myocardial function after myocardial infarction (4). (escardio.org)
  • iPSCs have great replicative ability and demonstrated potential to form functional cardiomyocytes (CMs). (creative-bioarray.com)
  • Creative Bioarray provides top quality service for directed-differentiation of iPSCs into cardiomyocytes using our proprietary induction protocol and reagents. (creative-bioarray.com)
  • 1. Lineage-committed cardiomyocytes differentiated from iPSCs. (creative-bioarray.com)
  • iPSCs show significant potential for cardiac regenerative medicine, as researchers can use biochemical cues to "program" them into a cardiomyocyte-like cell, which shows regenerative efficacy in damaged cardiac muscle. (medgadget.com)
  • 6- Differentiation of iPSCs into cardiomyocytes and endothelial cells for disease modeling and screening. (uh.edu)
  • "Ours is the first report to a DNA aptamer that allows cardiomyocytes to develop from iPSCs," explains Dr. Takaya when asked about the significance of the work. (shinshu-u.ac.jp)
  • A significant breakthrough in the use of 5-azacytidine for cell therapy and tissue engineering for CVD treatment has been gained based on its ability to differentiate mesenchymal stem cells into cardiomyocytes . (bvsalud.org)
  • Objective: To investigate the induction of tanshinone [1 A (Tan fl A) on the differentiation of human placenta-derived mesenchymal stem cells (hPDMSCs) into cardiomyocytes, and to provide an experimental basis for Tan IT A as a cardiomyocytc differentiation inducer. (researchgate.net)
  • Together, our work provides the scientific community with a powerful new genetic lineage tracing approach as well as a single cell transcriptomic atlas of hiPSCs undergoing cardiac differentiation. (stanford.edu)
  • Does not induce differentiation of vascular smooth muscle cells or endothelial cells. (tocris.com)
  • In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
  • Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
  • Dynamically and epigenetically coordinated gata/ets/sox transcription factor expression is indispensable for endothelial cell differentiation. (medicalxpress.com)
  • The protein GATA2, which binds to ETV2 and supports vascular endothelial differentiation, was induced immediately thereafter. (medicalxpress.com)
  • Transcription factors SOX and FLI1, both important for endothelial differentiation, were induced between 12 and 24 hours. (medicalxpress.com)
  • At 48 hours, after differentiation into vascular endothelium was determined, a system of transcription was established in which genes unique to vascular endothelial differentiation were induced. (medicalxpress.com)
  • Single-Cell Reconstruction and Mutation Enrichment Analysis Identifies Dysregulated Cardiomyocyte and Endothelial Cells in Congenital Heart Disease. (cdc.gov)
  • mEScell growth and differentiation on PLDLA-co-TMC membrane, demonstrated the cyto compatibility of this polymer, and provided evidence of minimal to no toxicity on the cells, allowing adhesion and cell proliferation, as well as differentiation into cardiomyocytes. (jscimedcentral.com)
  • Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. (tocris.com)
  • The practice of dentistry is likely to be revolutionized by biological therapies based on growth and differentiation factors that accelerate and/or induce a natural biological regeneration. (bvsalud.org)
  • the EST, which assesses the effects of compounds on the differentiation of mouse embryonic stem cells into contracting cardiomyocytes, can be used to rank the potency of chemicals within a series of alkoxyacetic acid metabolites formed from The implementation of the European REACH (Registration, glycol ethers. (cdc.gov)
  • 2022 ) Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages. (academictree.org)
  • The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
  • Furthermore, the decreased expression of the EZH2 gene is crucial for stem cell differentiation into specific cell lineages involved in myogenesis, adipogenesis, osteogenesis, neurogenesis, and haematopoiesis [ 9 ]. (hindawi.com)
  • What makes characterization challenging is that heterogeneity within a given stem cell population can cause variability in the differentiation into important downstream lineages, such as cardiomyocytes and hepatocytes. (genengnews.com)
  • Combined lineage tracing and scRNA-seq reveals unexpected first heart field predominance of human iPSC differentiation. (stanford.edu)
  • We deliver ready-to-use, functional cardiomyocytes that are desirable in vitro models for high content toxicity and drug screening, and cell regeneration research. (creative-bioarray.com)
  • Manuscript data set contains single cell RNA-sequencing data from in vitro hiPSC derived cardiomyocytes and includes both raw (fastq) and processed data (count matrix). (github.com)
  • 2021 ) Control of cardiomyocyte differentiation timing by intercellular signaling pathways. (academictree.org)
  • However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
  • An MC3T3-E1-based microarray dataset (accessioned as GSE46400) downloaded from the Gene Expression Omnibus included MC3T3-E1 cells with or without 14-day osteoblast differentiation osteoblast induction. (frontiersin.org)
  • Runx2 belongs to the Run family and is an essential upstream transcription factor during osteoblast differentiation. (frontiersin.org)
  • Osterix (OSX) is a transcription factor in the SP family, which is essential for osteoblast differentiation, and its expression is closely related to Runx2 ( 9 ). (frontiersin.org)
  • Recently, miRNA-messenger RNA (mRNA) regulatory relationships have been confirmed during biological processes, including osteogenic differentiation. (frontiersin.org)
  • This study aimed to find out more candidate miRNA-mRNA pairs involved in the osteogenic differentiation of MC3T3-E1 cells. (frontiersin.org)
  • The MC3T3-E1 cells were cultured and incubated in the osteogenic differentiation medium for 14 days. (frontiersin.org)
  • There were 7 overlapped miRNA-mRNA pairs identified during osteogenic differentiation of MC3T3-E1 cells, including mmu-miR-204-5p-Arhgap11a, mmu-miR-211-5p-Arhgap11a, mmu-miR-24-3p-H2afx, mmu-miR-3470b-Chek2, mmu-miR-3470b-Dlgap5, mmu-miR-466b-3p-Chek1, and mmu-miR-466c-3p-Chek1. (frontiersin.org)
  • The Arhgap11a, H2afx, Chek2, Dlgap5, and Chek1 were hub genes downregulated in MC3T3-E1 cells after osteogenic differentiation, verified by RT-qPCR results. (frontiersin.org)
  • Our study characterizes miR-211-5p targeting Arhgap11a promotes the osteogenic differentiation of MC3T3-E1 cells, which provides novel targets to promote the osteogenesis process during bone repair. (frontiersin.org)
  • Code for cardio scRNA-seq analysis and figure plots from manuscript A comprehensive analysis of gene expression changes in a high replicate and open-source dataset of differentiating hiPSC-derived cardiomyocytes . (github.com)
  • We compared this data with existing murine and 3D cardiac organoid scRNA-seq data and confirmed the dominance of left ventricular cardiomyocytes (>90%) in our hiPSC-derived progeny. (stanford.edu)
  • culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
  • Our Stem Cells review gives an overview of the use of small molecules in the control of stem cell growth & differentiation and somatic cell reprogramming. (tocris.com)
  • These include generation of knock-in reporter stem cell lines to study myogenic differentiation, CRISPR/Cas9 gene targeting methods to correct mutated genes in muscular dystrophies, and using bioscaffolds, seeded with stem cells, to treat skeletal muscle injuries. (uh.edu)
  • Differentiation of human epidermal neural crest stem cells (hEPI-NCSC) into virtually homogenous populations of DArgic neurons. (rndsystems.com)
  • The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
  • Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
  • Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
  • The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
  • Moreover, it seems that paracrine action of skeletal myoblasts facilitate neighboring cardiomyocytes to maintain their replicative potential and/or stimulate differentiation of native cardiac stem cells (8,9). (escardio.org)
  • I've also worked with differentiation from embryonic stem cells into interneurons. (lu.se)
  • During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
  • In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)
  • Previously, in the region that controls the function of the transcription factor that promotes differentiation from ES cells to a specific cell type, bivalent modifications of histones such as the accelerator and brake histone marks for transcription were thought to have coexisted. (medicalxpress.com)
  • hiPSCs were differentiated into cardiomyocytes using two protocols: small molecule (Lian et al. (github.com)
  • In ESD3, 6-gingerol severely affected differentiation into cardiomyocytes cell activity and protein content at both low and high concentrations. (nottingham.ac.uk)
  • In the earliest stages of development, the physical properties of the microenvironment can direct cell differentiation, and initiate the coordinated movement of groups of cells to establish the patterns that will define how the body is arranged. (mechanobio.info)
  • Cardiomyocytes are the chief cell type in the heart and their coordinated contraction as a mass is responsible for the pumping of blood around the developing embryo. (mechanobio.info)
  • Primary results on cell viability and cell morphology, as well as cardiomyocyte differentiation, have shown that PCL- gelatin scaffolds carrying 5-azacytidine developed in this work could serve as an ideal platform for mesenchymal stem cell differentiation into cardiomyocytes . (bvsalud.org)
  • At the Stem Cell Differentiation and Cytogenetics Group, we are interested in the effects of ionizing radiation on organs such as the brain, the heart, and the lung trying to elucidate the risks for patients treated with ionizing radiation as well as for astronauts, who are inevitably exposed to ionizing radiation on their missions into space. (gsi.de)
  • The ICOC has approved a very well-balanced portfolio of research proposals, including those aimed at understanding stem cell differentiation and identifying new ways of obtaining hESCs, and many that target specific diseases," Hall said. (ca.gov)
  • This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. (tocris.com)
  • A long-term multi-organ co-culture system coupled with quantitative imaging to visualize blood cell cycle progression, analyze polarized blood cell mitosis, and track blood cell differentiation kinetics during Drosophila hematopoiesis in homeostatic condition and following infection. (elifesciences.org)
  • Aptamers are single-stranded pieces of DNA that bind to target proteins and are able to modulate signaling cascades during cell differentiation when a stem cell commits to a specific functional role or phenotype. (shinshu-u.ac.jp)
  • Second, it results in the modulation of the Wnt signaling pathway that governs cell differentiation. (shinshu-u.ac.jp)
  • Ultimately, terminal cell differentiation commits to the cardiomyocyte lineage. (shinshu-u.ac.jp)
  • Interest in ALMS1 is heightened by the recent discovery of its involvement in neonatal cardiomyocyte cell cycle arrest, a process with potential relevance to regenerative medicine. (springer.com)
  • Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)
  • Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
  • These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
  • Is the Subject Area "Cell differentiation" applicable to this article? (plos.org)
  • Using ES cells developed at the Center for iPS Cell Research and Application (CiRA) in Kyoto University, the research group collected RNA and histones of each cell immediately after VEGF stimulation (0 h), before differentiation (6 h), during differentiation (12 - 24 h), and after differentiation (48 h). (medicalxpress.com)
  • Cell therapy is currently emerging as a potential new treatment for post MI patients with the assumption that recolonization of the areas of scarred myocardium with exogenously supplied surrogates or precursors of cardiomyocytes can restore function and ultimately affect clinical outcomes. (escardio.org)
  • We are investigating the mechanisms of skeletal muscle fiber-type differentiation. (seattlechildrens.org)
  • Myoblasts retain skeletal muscle properties and couple only sporadically to resident cardiomyocytes precluding synchronized contraction as mechanism of benefit (8). (escardio.org)
  • Surprisingly, our reporter system and scRNA-seq analysis revealed a predominance of FHF differentiation using the small molecule Wnt-based 2D differentiation protocol. (stanford.edu)
  • Using photolithography to shape a polydimethylsiloxane substrate and micropatterning to alter the substrate surface, the researchers developed 3D molds that mimic the natural forces and pressures that cardiomyocytes experience in the heart. (medgadget.com)
  • Biochemicalanalyses revealed that H9c2 cells showed different cardiomyocyte phenotypesand distinct levels of maturity depending on the amount of Ppy inthe substrate used. (ibecbarcelona.eu)
  • The expression of cardiac markers in iPSC-derived cardiomyocytes and the electrical properties of the cells. (creative-bioarray.com)
  • These lab-fabricated, fluorescently stained cardiomyocytes-heart muscle cells-exhibit the maturation and functionality of heart cells grown within a heart. (medgadget.com)
  • This results in more robust, mature and functional cardiomyocyte-like cells. (medgadget.com)
  • As the cells mature, they begin to beat, and more closely resemble mature, native cardiomyocytes. (medgadget.com)
  • Endocardium differentiation through Sox17 expression in endocardium precursor cells regulates heart development in mice. (nih.gov)
  • Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
  • The impairment of proliferation, differentiation, and mineralization in osteoblast is the main pathophysiological mechanism of osteoporosis, which has been proven by previous studies ( 4 , 5 ). (frontiersin.org)
  • In cardiac conditional Ezh2-knockout mice, cardiomyocyte proliferation was repressed, and the apoptosis process was induced [ 10 ]. (hindawi.com)
  • We are using biochemical and biomechanical cues to enhance the differentiation and maturation. (medgadget.com)
  • Alkaline phosphatase (ALP), osteocalcin (OCN), osteopontin (OPN), and runt-related transcription factor 2 (Runx2) are commonly used to evaluate differentiation and maturation of osteoblasts. (frontiersin.org)
  • In the process of blood vessel differentiation, the function of the protein ETS variant 2 (ETV2), which determines the differentiation into vascular endothelium, was first induced within 6 hours of differentiation stimulation. (medicalxpress.com)
  • The team developed a culture mold that mimics the physiological conditions under which cardiomyocytes grow. (medgadget.com)
  • Andreas Bruzelius defended his Ph.D. thesis 'Generating GABAergic interneurons through reprogramming and differentiation strategies' on Friday, 20 October 2023. (lu.se)
  • Poison Exon Splicing Regulates a Coordinated Network of SR Protein Expression during Differentiation and Tumorigenesis. (rndsystems.com)
  • Extracellular Matrix From Hypertrophic Myocardium Provokes Impaired Twitch Dynamics in Healthy Cardiomyocytes. (rndsystems.com)
  • Here, we describe the development of a TBX5/MYL2 lineage tracing reporter system that allows for the identification of FHF- progenitors and their descendants including left ventricular cardiomyocytes. (stanford.edu)
  • B) Human cardiomyocytes immunostained for α-Actinin (red). (gsi.de)