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  • 2018
  • Romano, Diego 2018-01-01 00:00:00 AbbreviationsBCEBacillus coagulans carboxylesterase 1CDcircular dichroismEtOAcethyl acetatehMGLhuman monoacylglycerol lipaseIPG1,2‐O‐isopropylideneglycerolpNPAp‐nitrophenyl acetateIntroductionCarboxylester hydrolases (EC 3.1.1.1) are enzymes that catalyze the cleavage or formation of carboxyl ester bonds, being often classified as esterases and lipases, based on experimental data and theoretical hypotheses. (deepdyve.com)
  • Detection
  • Upon addition of carboxylesterase, the latent probe could be easily hydrolyzed to liberate the fluorophore hydroxy hemicyanine and emit the off-on spectroscopic signal, which is desirable for obtaining sensitive detection with high specificity. (rsc.org)
  • Serum
  • The newly developed probe was successfully used to monitor and image carboxylesterase activity in complex biological samples such as serum, live cells and tissues. (rsc.org)
  • study
  • In this study, a new red lysosome-targeted fluorescence off-on probe has been rationally designed, synthesized and applied for the highly selective and sensitive fluorescence sensing of carboxylesterase in live cells, sera and tissues. (rsc.org)