For example, DNA ligase can join two complementary fragments of nucleic acid by forming phosphodiester bonds, and repair single stranded breaks that arise in double stranded DNA during replication. (wikipedia.org)
It is, as defined in biology, a class of enzyme that binds or joins two macromolecules by forming new bonds such as C-O, C-N, and C-S. Ligase enzyme catalyzes the ligase reaction, e.g. (changingyourbusiness.com)
Ligases are classified into six subclasses: (1) EC 6.1 (ligases that form carbon-oxygen bonds), (2) EC 6.2 (ligases that form carbon-sulfur bonds), (3) EC 6.3 (ligases that form carbon-nitrogen bonds), (4) EC 6.4 (ligases that form carbon-carbon bonds), (5) EC 6.5 (ligases that form phosphoric ester bonds), and ( 6) EC 6.6 (ligases that form nitrogen-metal bonds). (changingyourbusiness.com)
Multisite clickable modification of proteins using lipoic acid ligase. (ucdenver.edu)
Bacterial and fungal proteases are particularly important to the global carbon and nitrogen cycles in the recycling of proteins, and such activity tends to be regulated by nutritional signals in these organisms. (stratech.co.uk)
The net impact of nutritional regulation of protease activity among the thousands of species present in soil can be observed at the overall microbial community level as proteins are broken down in response to carbon, nitrogen, or sulfur limitation. (stratech.co.uk)
In general, a ligase catalyzes the following dehydration reaction, thus joining molecules A and B: A-OH + B-H → A-B + H2O The naming of ligases is inconsistent and so these enzymes are commonly known by several different names. (wikipedia.org)
Ligases are classified as EC 6 in the EC number classification of enzymes. (wikipedia.org)
Enzymes can be classified and named according to their reactions: (1) oxidoreductases, (2) transferases, (3) lyases, (4) isomerases, and (6) ligases. (changingyourbusiness.com)
Basically, ligases are a group of enzymes that enable the binding of two molecules. (changingyourbusiness.com)
Although the activities of most antioxidant and sulfur metabolism-related enzymes and glyoxalase I and the level of reduced glutathione in the Mg-deficient leaves and roots and the level of ascorbate in the leaves were kept in higher levels, the levels of malonaldehyde and MG and/or electrolyte leakage were increased in the Mg-deficient lower and upper leaves and roots, especially in the Mg-deficient lower leaves and roots. (biomedcentral.com)
Decreases in the levels of sugars and nitrogen-containing-compounds and in the activities of known 14-3-3-interacting-enzymes were observed in 14-3-3 overexpression plants. (biomedcentral.com)
Classified into six subclasses1
Based on the molecular bond catalyzed by the ligase enzyme, ligase can be classified into six subclasses (see Table 2). (changingyourbusiness.com)
Atoms4
The amino-modifier 5' Amino Modifier C6 with a spacer arm of 7 atoms (6 carbon and 1 nitrogen) is the simplest choice. (idtdna.com)
Stable carbon atoms that have the same atomic number as the element carbon, but differ in atomic weight. (lookformedical.com)
Isothiazolidine is a saturated, five-membered heterocycle containing three carbon atoms and a sulfur and nitrogen atom in the 1,2 positions. (chemenu.com)
Isothiazole or 1,2-thiazole is an organic compound containing a five-membered aromatic ring consisting of three carbon atoms, one nitrogen atom and one sulfur atom. (chemenu.com)
Nucleotide1
In gluconeogenic cells, aspartate is a carbon source for trehalose production, while in glycolytic cells using trehalose for carbon, aspartate is predominantly a nitrogen source for nucleotide synthesis. (elifesciences.org)
Compound2
The two-carbon acetyl group in acetyl-CoA is transferred to the four-carbon compound of oxaloacetate to form the six-carbon compound of citrate. (easyomics.org)
A tRNA modification balances carbon and nitrogen metabolism by regulating phosphate homeostasis. (instem.res.in)
Heterocyclic compounds2
Nitrogen-containing heterocyclic compounds such as 1,4-thiazine and its benzo derivatives are important compounds in many different research fields due to their remarkable biological properties. (chemenu.com)
Isothiazoles are an important class of five-membered sulfur heterocyclic compounds that are widely used in medicinal chemistry and organic synthesis due to the unique properties of two electronegative heteroatoms in the 1,2-relationship. (chemenu.com)
Generally, the common names of ligases include the word "ligase", such as in DNA ligase, an enzyme commonly used in molecular biology laboratories to join together DNA fragments. (wikipedia.org)
There are also some ligases that use the name "carboxylase" to indicate that the enzyme specifically catalyzes a carboxylation reaction. (wikipedia.org)
What Is The Role Of Dna Ligase In Dna Replication - An enzyme is a catalyst or chemical produced by cells to speed up a biochemical reaction. (changingyourbusiness.com)
Toxin Mutants Are Defective in Biofilm Ligase and Sphingomyelinase Activity, and Causation of Infective Endocarditis and Sepsis. (ucdenver.edu)
Bacteria1
A conserved insert in F. succinogenes Hsp60 that is commonly present in all bacterial species, except various Gram-positive bacteria, Deinococcus-Thermus groups and green non-sulphur bacteria, provides evidence that F. succinogenes does not belong to these taxa. (mcmaster.ca)
Organic1
Microbial electrosynthesis is a process in which microorganisms directly use electric current to reduce carbon dioxide to multi-carbon organic compounds that are excreted from the cells into extracellular medium[ 3 ]. (biomedcentral.com)
DNA Ligase is widely used in laboratories to perform DNA replication experiments. (changingyourbusiness.com)
Metabolites2
2017. Thiol trapping and metabolic redistribution of sulfur metabolites enable cells to overcome cysteine overload. . (instem.res.in)
This genome-scale model (iHN637) consisting of 637 genes, 785 reactions, and 698 metabolites captures all the major central metabolic and biosynthetic pathways, in particular pathways involved in carbon fixation and energy conservation. (biomedcentral.com)
Substrate2
Are you using the oligonucleotide as a substrate for DNA ligase? (idtdna.com)
5' phosphorylation is needed if an oligonucleotide will be used as a substrate for DNA ligase. (idtdna.com)
Dioxide1
The English "yeast" and the Dutch "gist" are derived from the Greek term zestos which means boiled, a reference to the bubbling foam caused by the evolution of carbon dioxide. (biologydiscussion.com)
Acid3
because, for example, amino acid-RNA ligase catalyzes, C-O bond formation between amino acid and RNA transfer. (changingyourbusiness.com)
Lipoic Acid Ligase-Promoted Bioorthogonal Protein Modification and Immobilization. (ucdenver.edu)
A ligase that makes the connection of two macromolecules and uses molecular energy obtained by the hydrolysis of nucleoside triphosphates (such as ATP, GTP, CTP, TTP, and UTP), for example, the hydrolysis of -adenosine triphosphate (ATP) is adenosine. (changingyourbusiness.com)
MeSH1
Ligases" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ucdenver.edu)
Commonly2
Commonly, the two terms are used interchangeably and are both used to describe ligases. (wikipedia.org)
The most commonly used ligase in the scientific laboratory is DNA ligase. (changingyourbusiness.com)
Biosynthesis1
In particular, upon entry into the stationary growth phase (which is often the consequence of starvation in one of the major cell metabolite supplies: carbon, nitrogen or phosphorus), we observed that polyamine biosynthesis was much affected, in parallel with the expression of S-adenosylmethionine decarboxylase [ 2 ]. (biomedcentral.com)
The Wood-Ljungdahl pathway of carbon fixation employed by acetogens is believed to be one of the most ancient metabolic pathways[ 5 ]. (biomedcentral.com)
Cycles1
Cycles, sources, and sinks: Conceptualizing how phosphate balance modulates carbon flux using yeast metabolic networks. (instem.res.in)
Our previous study suggests that three 14-3-3 isoforms (kappa, chi and psi) also play important roles in nitrogen and sulfur metabolic processes by regulating the activities of phosphoenolpyruvate carboxylase and O-acetylserine lyase [ 13 ]. (biomedcentral.com)
Source4
In that study, threshold amounts of trehalose - a limiting, produced carbon-resource, controls the emergence and self-organization of cells exhibiting the glycolytic state, serving as a carbon source that fuels glycolysis (Varahan et al. (elifesciences.org)
We have established that MTR is an excellent sulfur source in Bacillus subtilis [ 3 ]. (biomedcentral.com)
Methylthioribose (MTR) can be used by B. subtilis as the sole sulfur source. (biomedcentral.com)
This graph shows the total number of publications written about "Ligases" by people in this website by year, and whether "Ligases" was a major or minor topic of these publications. (ucdenver.edu)
Group1
5-Octadinynyl dU is a modified base with an 8-carbon linker terminating in an alkyne group and is the preferred way to insert alkynes at internal positions within a sequence. (idtdna.com)