• This enzyme belongs to the family of ligases, specifically those forming generic carbon-nitrogen bonds. (wikipedia.org)
  • Ligases, Forming carbon-nitrogen bonds, Acid--D-amino-acid ligases (peptide synthases). (uma.es)
  • It was found that this artificial RNA ligase has higher thermostability than natural RNA ligase as well as superior ligation activity for RNA fragments containing xenonucleic acid. (vietreader.com)
  • Arginyl-tRNA synthetase (Arginine--tRNA ligase) (ArgRS) [Ensembl]. (ntu.edu.sg)
  • Our work identified a novel mammalian prenyltransferase which we named GGtase3 that modifies FBXL2, an E3 ligase subunit, with geranylgeranylation (a 20-carbon lipid PTM) for localization to membrane compartments. (kuchaylab.org)
  • Our study provides the first global insight into the molecular physiological response of a multicellular fungus to carbon catabolite regulation and identifies several not yet known targets in a growth-controlled environment. (biomedcentral.com)
  • For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. (lbl.gov)
  • The systematic name of this enzyme class is ribose-5-phosphate:ammonia ligase (ADP-forming). (wikipedia.org)
  • In enzymology, a ribose-5-phosphate-ammonia ligase (EC 6.3.4.7) is an enzyme that catalyzes the chemical reaction ATP + ribose 5-phosphate + NH3 ⇌ {\displaystyle \rightleftharpoons } ADP + phosphate + 5-phosphoribosylamine The 3 substrates of this enzyme are ATP, ribose 5-phosphate, and NH3, whereas its 3 products are ADP, phosphate, and 5-phosphoribosylamine. (wikipedia.org)
  • Carbon dioxide (CO2) is produced during respiration by all animals, fungi and microorganisms. (ymdb.ca)
  • Gluten chains in the bread hold the carbon dioxide in creating the airiness of the bread. (ymdb.ca)
  • Cell surface proteins tagged with a 15-amino acid peptide are biotinylated by Escherichia coli biotin ligase (BirA), whereas endogenous proteins are not modified. (ox.ac.uk)
  • One such wide-domain regulatory circuit, essential to all cells, is carbon catabolite repression (CCR): it allows the cell to prefer some carbon sources, whose assimilation is of high nutritional value, over less profitable ones. (biomedcentral.com)
  • One such wide-domain regulatory circuit is carbon catabolite repression (CCR): it allows the preferred assimilation of carbon sources of high nutritional value over others [ 1 - 4 ]. (biomedcentral.com)
  • Genes required for degradation of and growth on the carbon source pectin were down-regulated, whereas transcripts involved in redox processes were up-regulated. (frontiersin.org)
  • Often substrate degradation via ubiquitin ligases is regulated for precision and fine control. (kuchaylab.org)
  • We seek to elucidate the spatiotemporal mechanistic understating for the specificity of the ubiquitylation reaction by E3 ubiquitin ligases. (kuchaylab.org)
  • When using the natural RNA ligase for RNA fragments (including xenonucleic acid) as reaction substrates to synthesize a marketed siRNA drug substance, the yield was only 20% after 24 hours of reaction. (vietreader.com)
  • In contrast, when using the highly functional artificial RNA ligase, the reaction yield improved to 80% under the same conditions, confirming that it has properties suitable for enzymatic synthesis of nucleic acid medicines. (vietreader.com)
  • Expression of Formate-Tetrahydrofolate Ligase Did Not Improve Growth but Interferes With Nitrogen and Carbon Metabolism of Synechocystissp. (mpg.de)
  • Growth on CO requires the enzyme carbon monoxide dehydrogenase (CODH) that catalyzes the reversible conversion between CO and CO 2 . (hindawi.com)
  • Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin. (ox.ac.uk)
  • In that study, threshold amounts of trehalose - a limiting, produced carbon-resource, controls the emergence and self-organization of cells exhibiting the glycolytic state, serving as a carbon source that fuels glycolysis (Varahan et al. (elifesciences.org)
  • In gluconeogenic cells, aspartate is a carbon source for trehalose production, while in glycolytic cells using trehalose for carbon, aspartate is predominantly a nitrogen source for nucleotide synthesis. (elifesciences.org)
  • Although, PTMs on substrates are well studied, PTMs on E3 ligases and how they regulate the UPS are understudied. (kuchaylab.org)
  • This metabolic plasticity of aspartate enables carbon-nitrogen budgeting, thereby driving the biochemical self-organization of distinct cell states. (elifesciences.org)
  • This is usually achieved through inhibition of gene expression of enzymes involved in the catabolism of other carbon sources than the preferred ones. (biomedcentral.com)
  • Here we chose to use chemostat cultures on D-glucose as a carbon source at two different growth rates (one repressing and one derepressing [ 16 ]) to investigate the genome-wide changes in gene expression in relation to CRE1 function, using a Δcre1 recombinant mutant strain of T. reesei and corresponding control strain. (biomedcentral.com)
  • Carboxidotrophs grow chemolitoautotrophically on carbon monoxide (CO) and are considered to hold a vital niche in terrestrial and marine thermophilic ecosystems [ 1 ]. (hindawi.com)
  • We are currently involved in functional characterization of GGtase3 and extending our studies for functional characterization of PTMs involving membrane bound E3 ubiquitin ligases. (kuchaylab.org)
  • In collaboration with researchers from the University of Shizuoka, Aji Bio-Pharma succeeded in developing a highly functional artificial RNA ligase using the ancestral design method. (vietreader.com)
  • Ubiquitin--protein ligase. (uma.es)
  • Enzymes that catalyze the joining of two molecules by the formation of a carbon-oxygen bond. (nih.gov)
  • According to the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology ( NC-IUBMB ) classification, EC 6 ligases are enzymes that catalyse the joining of two molecules with simultaneous hydrolysis of of the diphosphate bond in triphosphate, usually ATP. (me.uk)
  • These enzymes catalyze the breakage of a carbon-oxygen bond in polysaccharides leading to an unsaturated product and the elimination of an alcohol. (lookformedical.com)
  • Enzymes that catalyze the cleavage of a carbon-carbon bond of a 3-hydroxy acid. (lookformedical.com)
  • Calculated flux distributions under different conditions show that a number of metabolic pathways are affected by the activity of some metabolic enzymes at key nodes in metabolism, including the transport mechanism of carbon sources and the ability to fix carbon dioxide. (biomedcentral.com)
  • We discovered that transient depletion of biotin protein ligase (BPL) and thioredoxin reductase (TrxB2) results in latent infections during which Mtb cannot be detected but that relapse in a subset of mice. (nih.gov)
  • Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • This graph shows the total number of publications written about "Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor" by people in Harvard Catalyst Profiles by year, and whether "Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor" was a major or minor topic of these publication. (harvard.edu)
  • Below are the most recent publications written about "Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor" by people in Profiles. (harvard.edu)
  • 2001). The common steps of phenylpropanoid pathway are catalyzed by phenylalanine ammonia- lyase (PAL), cinnamate 4-hydroxylase (C4H) and 4-coumarate:CoA ligase (4CL). (scielo.br)
  • Awakening a latent carbon fixation cycle in Escherichia coli. (mpg.de)
  • This study, titled "Assimilation of Formic Acid and CO 2 by Engineered Escherichia coli Equipped with Reconstructed One-Carbon Assimilation Pathways," has been published online in the Proceedings of the National Academy of Sciences on September 18. (phys.org)
  • Junho Bang et al, Assimilation of formic acid and CO 2 by engineered Escherichia coli equipped with reconstructed one-carbon assimilation pathways, Proceedings of the National Academy of Sciences (2018). (phys.org)
  • Catalytic asymmetric addition of carbon nucleophiles (donor) to ketones (acceptor) is a fundamental approach to construct new tetrasubstituted stereogenic carbon centers. (biomedcentral.com)
  • Formic acid and CO 2 conversion to pyruvate was increased via metabolic engineering of the E. coli strain equipped with the one-carbon assimilation pathway. (phys.org)
  • In this report, a one-carbon pathway that can efficiently convert formic acid and CO 2 to pyruvate was developed and a cellular energy producing pathway from formic acid was introduced. (phys.org)
  • For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. (lbl.gov)
  • Therefore, biosynthesis of branched-chain sugar from low-cost and abundant carbon sources is essential. (biomedcentral.com)
  • For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code . (lbl.gov)
  • The reconstructed one-carbon pathways can supply cellular molecules and cellular energies from the formic acid and CO 2 . (phys.org)
  • 11. RNF4, a SUMO-targeted ubiquitin E3 ligase, promotes DNA double-strand break repair. (nih.gov)
  • 15. Ataxin-3 consolidates the MDC1-dependent DNA double-strand break response by counteracting the SUMO-targeted ubiquitin ligase RNF4. (nih.gov)
  • The enzymatic synthesis of 5-amino-1-ribosyl-4-imidazolecarboxylic acid 5′-phosphate from 5-amino-1-ribosylimidazole 5′-phosphate and carbon dioxide. (opensourcebiology.eu)
  • Moreover, the cost-effective synthesis of dendroketose starting from one-, three-, and six-carbon resources was achieved through in vivo and in vitro metabolic engineering strategies. (biomedcentral.com)
  • Additionally, the dendroketose production reached 36.3 g/L with a yield of 0.46 mol/mol glucose when glucose served as the sole carbon resource. (biomedcentral.com)
  • This resulted in an engineered E. coli strain that can efficiently utilize formic acid as a carbon source while glucose consumption was reduced. (phys.org)
  • It differs from CHONDROITIN SULFATE A (see CHONDROITIN SULFATES) by containing IDURONIC ACID in place of glucuronic acid, its epimer, at carbon atom 5. (lookformedical.com)
  • A research group at KAIST has developed an engineered E. coli strain that converts formic acid and CO 2 to pyruvate and produces cellular energy from formic acid through reconstructed one-carbon pathways. (phys.org)
  • Formic acid is a carboxylic acid composed of one carbon. (phys.org)
  • Recently, the C1 Gas Refinery R&D Center has successfully developed a biological process that produces formic acid from carbon monoxide for the first time. (phys.org)
  • There has been increasing interest in utilizing formic acid as an alternative carbon source for the production of value-added chemicals. (phys.org)
  • This research reports the development of an engineered E. coli strain that can convert formic acid and CO 2 to pyruvate and produce cellular energy from formic acid through the reconstructed one-carbon pathways. (phys.org)
  • This sub-class of ligases usually named as carboxylases. (me.uk)
  • The 3 and 5 designations refer to the position of carbon atoms on the ring structure of the deoxyribose molecule. (nih.gov)
  • Utilization of Flue Gas as a Carbon Dioxide Source from a Coal-Burning" by Swaminathan Palanisami, Thiruvenkadam Viswanathan et al. (mst.edu)
  • Flue gas originating from a coal-fired power plant was used as a carbon dioxide source for microalgae cultivation. (mst.edu)
  • Utilization of Flue Gas as a Carbon Dioxide Source from a Coal-Burning Power Plant for the Cultivation of Microalgae in Outdoor Circular Ponds," International Journal of Pharma and Bio Sciences , vol. 4, no. 4, pp. (mst.edu)
  • Therefore, it can be used as an alternative carbon source in the microbial fermentation process. (phys.org)
  • The tetrahydrofolate cycle was reconstructed by utilizing Methylobacterium extorquens formate-THF ligase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase. (phys.org)
  • The strategy described in this study provides a new platform for producing value-added chemicals from one-carbon sources. (phys.org)