• This enzyme belongs to the family of ligases, specifically those forming carbon-sulfur bonds as acid-thiol ligases. (wikipedia.org)
  • It is, as defined in biology, a class of enzyme that binds or joins two macromolecules by forming new bonds such as C-O, C-N, and C-S. Ligase enzyme catalyzes the ligase reaction, e.g. (changingyourbusiness.com)
  • Ligases are classified into six subclasses: (1) EC 6.1 (ligases that form carbon-oxygen bonds), (2) EC 6.2 (ligases that form carbon-sulfur bonds), (3) EC 6.3 (ligases that form carbon-nitrogen bonds), (4) EC 6.4 (ligases that form carbon-carbon bonds), (5) EC 6.5 (ligases that form phosphoric ester bonds), and ( 6) EC 6.6 (ligases that form nitrogen-metal bonds). (changingyourbusiness.com)
  • Ligases, Forming carbon-nitrogen bonds, Acid--D-amino-acid ligases (peptide synthases). (uma.es)
  • In enzymology, a phenylacetate-CoA ligase is an enzyme (EC 6.2.1.30) that catalyzes the chemical reaction ATP + phenylacetate + CoA ⇌ {\displaystyle \rightleftharpoons } AMP + diphosphate + phenylacetyl-CoA The 3 substrates of this enzyme are ATP, phenylacetate, and CoA. (wikipedia.org)
  • The systematic name of this enzyme class is phenylacetate:CoA ligase (AMP-forming). (wikipedia.org)
  • Enzymes can be classified and named according to their reactions: (1) oxidoreductases, (2) transferases, (3) lyases, (4) isomerases, and (6) ligases. (changingyourbusiness.com)
  • Basically, ligases are a group of enzymes that enable the binding of two molecules. (changingyourbusiness.com)
  • There are six types of enzymes and ligases are one of them. (changingyourbusiness.com)
  • Ligase and lyase are closely related classes of enzymes. (changingyourbusiness.com)
  • However, ligase and lyase are different types of enzymes. (changingyourbusiness.com)
  • Although the activities of most antioxidant and sulfur metabolism-related enzymes and glyoxalase I and the level of reduced glutathione in the Mg-deficient leaves and roots and the level of ascorbate in the leaves were kept in higher levels, the levels of malonaldehyde and MG and/or electrolyte leakage were increased in the Mg-deficient lower and upper leaves and roots, especially in the Mg-deficient lower leaves and roots. (biomedcentral.com)
  • Multisite clickable modification of proteins using lipoic acid ligase. (ucdenver.edu)
  • By binding to the adaptor protein SKP1 and serving as substrate receptors for the Skp1, Cullin, F-box E3 ubiquitin ligase complex, F-box proteins regulate critical cellular processes including cell cycle progression and membrane trafficking. (uga.edu)
  • because, for example, amino acid-RNA ligase catalyzes, C-O bond formation between amino acid and RNA transfer. (changingyourbusiness.com)
  • Microbial electrosynthesis is a process in which microorganisms directly use electric current to reduce carbon dioxide to multi-carbon organic compounds that are excreted from the cells into extracellular medium[ 3 ]. (biomedcentral.com)
  • They have found that biological communities are thriving in a chemical soup rich in hydrogen, carbon dioxide and sulfur. (bartleby.com)
  • Our team studies the regulation and cellular functions of the sulfur metabolism, in particular during adaptation to starvation for sulfur nutrients. (cea.fr)
  • The sulfur metabolism ( fig 1 ) is highly conserved across species and occupies a position apart in the cellular function and homeostasis owing to the reactivity of thiols and the characteristics of the two sulfur-containing amino acids, methionine and cysteine. (cea.fr)
  • The sulfur metabolism is tightly regulated to fit cell requirements as lack or over-accumulation of certain sulfur-containing compounds have dramatic impacts on cell physiology, organism's health and ageing. (cea.fr)
  • The sulfur-metabolism is a complex and integrated process controlled at numerous levels. (cea.fr)
  • We also show that MtnK synthesis is boosted under starvation condition, in the following decreasing order: carbon-, sulfur- and nitrogen-starvation. (biomedcentral.com)
  • In particular, upon entry into the stationary growth phase (which is often the consequence of starvation in one of the major cell metabolite supplies: carbon, nitrogen or phosphorus), we observed that polyamine biosynthesis was much affected, in parallel with the expression of S-adenosylmethionine decarboxylase [ 2 ]. (biomedcentral.com)
  • The main interest of our team is how cells respond and adapt to ensure survival over periods of starvation for sulfur nutrients . (cea.fr)
  • Indeed, when yeast cells are submitted to starvation for sulfur nutrients, they stop dividing but remain viable for weeks. (cea.fr)
  • Our objective is to identify these mechanisms and to understand, at the cellular and molecular levels, how they contribute to cell survival during sulfur starvation. (cea.fr)
  • The capacity of yeast to resist sulfur starvation also raises the underlying question of the role of the sulfur compounds in the cell physiology and function. (cea.fr)
  • What are the molecular actors required for survival and recovery under sulfur starvation? (cea.fr)
  • Is there a redistribution/reallocation of the cellular pool of sulfur compounds during starvation? (cea.fr)
  • A ligase that makes the connection of two macromolecules and uses molecular energy obtained by the hydrolysis of nucleoside triphosphates (such as ATP, GTP, CTP, TTP, and UTP), for example, the hydrolysis of -adenosine triphosphate (ATP) is adenosine. (changingyourbusiness.com)
  • Based on the molecular bond catalyzed by the ligase enzyme, ligase can be classified into six subclasses (see Table 2). (changingyourbusiness.com)
  • A conserved insert in F. succinogenes Hsp60 that is commonly present in all bacterial species, except various Gram-positive bacteria, Deinococcus-Thermus groups and green non-sulphur bacteria, provides evidence that F. succinogenes does not belong to these taxa. (mcmaster.ca)
  • This genome-scale model (iHN637) consisting of 637 genes, 785 reactions, and 698 metabolites captures all the major central metabolic and biosynthetic pathways, in particular pathways involved in carbon fixation and energy conservation. (biomedcentral.com)
  • Sulfur metabolites are essential for many cellular processes including protein synthesis, methylation reactions, redox equilibrium and xenobiotic detoxification. (cea.fr)
  • Growth on CO requires the enzyme carbon monoxide dehydrogenase (CODH) that catalyzes the reversible conversion between CO and CO 2 . (hindawi.com)
  • Are you using the oligonucleotide as a substrate for DNA ligase? (idtdna.com)
  • 5' phosphorylation is needed if an oligonucleotide will be used as a substrate for DNA ligase. (idtdna.com)
  • Carboxidotrophs grow chemolitoautotrophically on carbon monoxide (CO) and are considered to hold a vital niche in terrestrial and marine thermophilic ecosystems [ 1 ]. (hindawi.com)
  • Altogether these regulations contribute to maintain the pool of essential sulfur-compounds at physiological levels regardless variations of sulfur sources and their availability in the cellular environment. (cea.fr)
  • Is there an interplay between antioxidative sulfur compounds (GSH) and autophagy (knowing its role in elimination of damage molecules) in the protection against oxidative stress injuries and ageing diseases? (cea.fr)
  • The latter is an excellent sulfur source and the precursor of quorum-sensing signalling molecules. (biomedcentral.com)
  • The two-carbon acetyl group in acetyl-CoA is transferred to the four-carbon compound of oxaloacetate to form the six-carbon compound of citrate. (easyomics.org)
  • Lipoic Acid Ligase-Promoted Bioorthogonal Protein Modification and Immobilization. (ucdenver.edu)
  • Pereira et al 2008), (iii) a dynamic control of the degradation of GSH which is also a sulfur storage compound (Baudouin-Cornu et al 2012). (cea.fr)
  • Based on the successful experience of CuAAC and the further development of click chemistry, the research group of K. B. Sharpless focused on the synthesis of hexavalent sulfur fluorides and their unique reactivity. (huatengsci.com)
  • Toxin Mutants Are Defective in Biofilm Ligase and Sphingomyelinase Activity, and Causation of Infective Endocarditis and Sepsis. (ucdenver.edu)
  • The fragments that were analyzed by Muck were found to be functional matches to an RNA ligase and a queuine tRNA-ribosyltransferase (NCBI). (bartleby.com)
  • Ligases" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ucdenver.edu)
  • The Wood-Ljungdahl pathway of carbon fixation employed by acetogens is believed to be one of the most ancient metabolic pathways[ 5 ]. (biomedcentral.com)
  • These studies will help to better understand the impact of severe nutritional (sulfur) stress on the cell behaviour and function, as well as their consequences on cellular health. (cea.fr)
  • What Is The Role Of Dna Ligase In Dna Replication - An enzyme is a catalyst or chemical produced by cells to speed up a biochemical reaction. (changingyourbusiness.com)
  • In 2014, the group of K. B. Sharpless reported a click chemistry reaction based on Sulfur(VI) fluoride exchange (SuFEx), which is another representative reaction in click chemistry. (huatengsci.com)
  • The cofactors by which ancillary download transformation of surfaces 1914 holds generated to events and these remain increased up from the pancreas degree into steps where they are composed to cleave suppressor or cyclized in active devices are a biological proton of common view and ligases in them can translocate to old hydroxylation. (erik-mill.de)
  • Uni-Link™ is an amino-modifier phosphoramidite from Clontech that provides a free primary amine attached to the 5'-end of an oligo via a six carbon aliphatic spacer arm. (idtdna.com)
  • Biogeochemical data indicated that the decomposition of native organic matter occurred in different phases, beginning with mineralization of dissolved organic matter (DOM) to CO 2 during the first week of incubation, followed by a pulse of acetogenesis that dominated carbon flux after 2 weeks. (frontiersin.org)
  • 5-Octadinynyl dU is a modified base with an 8-carbon linker terminating in an alkyne group and is the preferred way to insert alkynes at internal positions within a sequence. (idtdna.com)
  • 5-Octadiynyl dU is a modified base with an eight carbon linker terminating in an alkyne group. (idtdna.com)
  • Other names in common use include phenylacetyl-CoA ligase, PA-CoA ligase, and phenylacetyl-CoA ligase (AMP-forming). (wikipedia.org)
  • A pulse of methanogenesis co-occurred with acetogenesis, but only accounted for a small fraction of carbon flux. (frontiersin.org)
  • This graph shows the total number of publications written about "Ligases" by people in this website by year, and whether "Ligases" was a major or minor topic of these publications. (ucdenver.edu)