Loading...
  • viruses
  • Self-assembly of viruses seems to obey the principles of thermodynamically reversible self-assembly but assembled shells ('capsids') strongly resist disassembly. (asknature.org)
  • Capsid proteins from other DNA viruses were also shown to activate the Ad E1A promoter independent of Ad gene expression and support virus replication. (nih.gov)
  • Viruses with mutated PPxY motif in protein VI (M1) have altered gene expression. (nih.gov)
  • Post-translational modifications in viral capsids are known to fine-tune and regulate several aspects of the infective life cycle of several viruses in the host. (nih.gov)
  • By using sequence alignment and modeling techniques, this lineage was recently extended to include additional large-faceted viruses containing a double-barrel trimeric major coat protein ( 4 ). (pnas.org)
  • major
  • VP4 lies on the inner surface of the protein shell formed by the major capsid proteins, VP1, VP2 and VP3. (ebi.ac.uk)
  • The three major capsid proteins have a conserved beta-barrel fold, while VP4 has little regular secondary structure. (ebi.ac.uk)
  • We report here the crystal structure of the major capsid protein (MCP) of the Sulfolobus turreted icosahedral virus, an archaeal virus isolated from an acidic hot spring (pH 2-4, 72-92°C) in Yellowstone National Park. (pnas.org)
  • Although some observed bound structures here closely resemble the major binding interfaces in the capsid assembly, they are statistically insignificant in our simulation trajectories. (iupui.edu)
  • Identification of the major capsid protein gene of human cytomegalovirus. (asm.org)
  • The coding region for the major capsid protein (MCP) of human cytomegalovirus (HCMV) was identified by comparing the protein sequence with the respective sequences of herpes simplex virus (HSV), Epstein-Barr virus, and varicella-zoster virus. (asm.org)
  • Polyclonal
  • A) U2OS cells were infected with HH-Ad5-VI-wt at a MOI of 1000 FFU/cell and fractionated at 20 min intervals and subjected to IB using serum against protein VI, polyclonal Ab (pAb) against the splicing factor SAF-A (nuclear fraction) and monoclonal Ab (MAb) against β-actin (cytoplasmic fraction) as indicated to the right. (nih.gov)
  • A portion of the open reading frame was expressed in Escherichia coli plasmid pBD2 IC2OH as a beta-galactosidase fusion protein and was used to generate polyclonal antibodies in New Zealand White rabbits. (asm.org)
  • UniProt
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)
  • strain
  • In the strain UCD 67-385, these both proteins were identified as viral capsid protein (CP), allow to confirm the gag predicted ORFs in XdV-L1A, XdV-L1B, and XdV-L2, with CPs of 76.6, 76.2, and 38.8 kDa, respectively. (springer.com)
  • Selenomethionine (SeMet)-labeled protein was expressed in the Escherichia coli BL21 Star (DE3) One Shot (Invitrogen) strain carrying a Rosetta vector (Novagen) by using the Met biosynthesis inhibition protocol ( 5 ). (pnas.org)
  • A monoclonal antibody against the human MCP and a monospecific rabbit antiserum against strain Colburn of simian cytomegalovirus detected the fusion protein as well as the MCP of purified virions in immunoblots. (asm.org)
  • assembly
  • Experiments using a series of mutants defective for proper processing and assembly of capsid yielded evidence suggesting that the restriction factor binding site is in fact formed only when capsid is in its polymeric state in a mature virus, thus explaining why conventional approaches had failed to detect any interaction. (bl.uk)
  • affinity
  • Protein purification was carried out at room temperature by using affinity and ion-exchange chromatography. (pnas.org)
  • To help understand how the HIV CA proteins self-organize into large assemblies of various shapes, we aim to computationally evaluate the binding affinity and interfaces in a CA homodimer. (iupui.edu)
  • Virus
  • Human immunodeficiency virus (HIV) capsid proteins, computer artwork. (sciencephoto.com)
  • Viral determinants of this restriction map to the virus capsid protein, however despite strong genetic ev idence, no direct interaction has been shown between capsid and restriction factor using a variety of biochemical approaches. (bl.uk)
  • Green: Capsid Protein (Dengue virus) protein stained by Capsid Protein (Dengue virus) antibody (GTX124247) diluted at 1:2000. (genetex.com)
  • Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis. (genetex.com)
  • Endogenous PML (first column) and virus, derived protein VI (second column), were detected with specific Ab. (nih.gov)
  • The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. (uniprot.org)
  • The capsid architecture of STIV is reminiscent of the mammalian adenovirus, bacteriophage PRD1, and Paramecium bursaria Chlorella virus (PBCV-1). (pnas.org)
  • Capsid protein (CA) is the building block of virus coats. (iupui.edu)
  • The Zika Virus Capsid Protein can be used for a variety of research applications. (genwaybio.com)
  • synthesis
  • We use the natural the protein synthesis 'machinery' in micro-organisms to create novel protein-like polymers that consist of a string of polymer segments (also called 'modules' or blocks), each with a different function, that are often (but not always) inspired by (or copied from) natural proteins. (wur.nl)
  • structure
  • Several antigenic sites were identified and mapped onto the three-dimensional protein structure. (dovepress.com)
  • The MCP structure also provides insights into the stabilizing forces required for extracellular hyperthermophilic proteins to tolerate high-temperature hot springs. (pnas.org)
  • We further analyze the capsid architecture of STIV by docking the MCP crystal structure into the cryo-EM reconstruction and calculating a difference map. (pnas.org)
  • The MCP (an extracellular protein) structure also suggests how proteins may be able to tolerate the extreme physicochemical habitat of high-temperature hot spring environments. (pnas.org)
  • Literature
  • p>An evidence describes the source of an annotation, e.g. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc. (uniprot.org)
  • machinery
  • Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. (uniprot.org)
  • fold
  • To further validate this, we disrupted a glycosylation site identified in AAV2 capsid (AAV2-N253Q), which severely compromised its packaging efficiency (~ 100-fold vs. AAV2 wild-type vectors). (nih.gov)