The method exhibited superior separation resolution for protein complexes than conventional native capillary zone electrophoresis (nCZE-MS). In our study, ncIEF-MS achieved liquid-phase separations and MS characterization of seven different forms of a streptavidin homotetramer with variations of N-terminal methionine removal, acetylation, and formylation and four forms of the carbonic anhydrase-zinc complex arising from variations of PTMs (succinimide, deamidation, etc. (bvsalud.org)
Title: Stability-indicating capillary zone electrophoresis method for the assessment of recombinant human granulocyte-macrophage colony-stimulating factor and its correlation with reversed-phase liquid chromatography method and bioassay. (aablocks.com)
Separation6
The thesis focuses on the development of new homogeneous gels for the separation of drug enantiomers, peptides, DNA and virus by electrophoresis and electrochromatography in capillaries and microchips. (dissertations.se)
Title: Separation of fluorescently labeled phosphoinositides and sphingolipids by capillary electrophoresis. (aablocks.com)
Title: Separation and determination of chiral composition in penicillamine tablets by capillary electrophoresis in a broad pH range. (aablocks.com)
Baczek T., Walijewski L., Kaliszan R.: pH gradient reversed-phase liquid chromatography as a fractionation tool for the separation of peptides. (edu.pl)
Michel M., Baczek T., Studzinska S., Bodzioch K., Jonsson T., Kaliszan R., Buszewski B.: Comparative evaluation of high-performance liquid chromatography stationary phases used for the separation of peptides in terms of quantitative structure-retention relationships. (edu.pl)
A chiral separation mannequin of gel electrochromatography in a polydimethylsiloxane (PDMS) microfluidic system for amino acids (AAs) is introduced. (teitell-lab.com)
Enantiomers1
An EKC methodology for the willpower of ornithine (Orn) enantiomers has been developed after a quick pre-capillary derivatization with FITC. (teitell-lab.com)
Chromatography1
Baczek T., Kaliszan R.: Predictions of peptides' retention times in reversed-phase liquid chromatography as a new supportive tool to improve protein identification in proteomics. (edu.pl)
Proteins2
Here we discuss two approaches currently used to investigate the global dynamics of phosphorylation based on the isolation of phosphorylated proteins or peptides. (acs.org)
Mass spectrometry is an indispensable field for analyzing biomolecules like Analysis of Glycans, Analysis of Lipids, Analysis of Proteins and Peptides, Analysis of Oligonucleotides. (longdom.org)
Quantitative1
Recent advances in peptide-based (bottom-up) quantitative proteomics and bioinformatics have opened unprecedented opportunities for extensive investigation of cellular proteomes and their dynamics. (acs.org)
Analysis1
For this reason, we investigated the potential of image capillary isoelectric focusing (icIEF) combined with Principal Component Analysis (PCA) and Partial least squares-discriminant analysis (PLS-DA). (bvsalud.org)
Method1
To characterize the charge heterogeneity of a protein, imaged capillary isoelectric focusing (icIEF) has become the gold standard method. (bvsalud.org)
Potential1
Macur K., Baczek T., Kaliszan R., Temporini C., Corana F., Massolini G., Grzenkowicz-Wydra J., Obuchowski M.: Correctness of protein identifications of bacillus subtilis proteome with the indication on potential false positive peptides supported by predictions of their retention times. (edu.pl)