• PROSTVAC-F-TRICOM is a cancer vaccine consisting of a recombinant fowlpox virus encoding PSA and TRICOM (a combination of B7.1, ICAM-1, and LFA-3), which induces antitumor immune response against PSA-positive tumors ( PMID: 16390546 ). (jax.org)
  • Vaccinia virus, a member of the Poxviridae family, has been extensively used as an oncolytic agent and has entered late stage clinical development. (oncotarget.com)
  • Vaccinia virus (VACV) is one of the best studied representative of the Orthopoxvirus genus. (oncotarget.com)
  • The TK-disrupted control vaccinia virus designated V69 was constructed by generating the recombinant plasmid pGS69, which contains the influenza A/PR/8/34 nucleoprotein in flanking TK gene segments and lacks the gene (24). (tech-strategy.org)
  • Q.D.), or recombinant murine IL-10 administration alone (PeproTech, Inc.). Pooled sera from two immunized mice were obtained 2, 4, 6, 14, and 21 days after treatment and were analyzed by enzyme-linked immunosorbent assay for the presence of antibodies (Abs) against -gal protein or wild-type vaccinia virus. (tech-strategy.org)
  • Briefly, microtiter plates were dried down overnight at 37C in a nonhumidified incubator with 200 ng/well/50 l of purified -gal protein (Sigma Chemical Co., St. Louis, MO, U.S.A.). Alternatively, microtiter plates were coated with wild-type vaccinia virus (WT-VV) (5 105/well/50l) at 4C, overnight. (tech-strategy.org)
  • Using rFWPV containing lacZ under the control of a vaccinia virus (VV) early/late promoter (p7.5K) and a 10 plaque-forming units per cell multiplicity of infection, >80% of cells expressed the lacZ marker gene. (lookformedical.com)
  • Negri bodies are found in cells infected with________: A. Fowlpox virus B. Vaccinia virus C. Rabies virus D. Paramyxo viruses 18. (pdfcoffee.com)
  • This polyprotein was expressed by a plasmid DNA vaccine vector (DNA) and by two attenuated poxvirus vectors, modified vaccinia virus Ankara (MVA) and fowlpox virus of the FP9 strain. (ox.ac.uk)
  • Siccardi's areas of research comprise HIV, influenza, cancer (especially mesothelioma), immunoglobulin E, and vaccine design of recombinant Modified Vaccinia Ankara virus (MVA) or Fowlpoxvirus. (wikipedia.org)
  • Thus, IL-10 enhanced the function of a recombinant poxvirus-based anti-cancer vaccine and may represent a potential NVP-QAV-572 adjuvant in the vaccination against human cancers using recombinant poxvirus-based vaccines. (tech-strategy.org)
  • Determine the maximum tolerated dose and dose-limiting toxic effects of recombinant fowlpox-TRICOM vaccine in patients with advanced squamous cell carcinoma of the oral cavity or oropharynx or nodal or dermal metastases. (knowcancer.com)
  • A canarypox vaccine expressing multiple human immunodeficiency virus type 1 genes given alone or with rgp120 elicits broad and durable CD8+ cytotoxic T lymphocyte responses in seronegative volunteers. (lookformedical.com)
  • Dr. Evans works in collaboration with colleagues and post-doctoral fellows to understand complex mechanisms in virus-host interactions with goals toward vaccine development. (scitechnol.com)
  • Dr. Evans has also expanded his vaccine research efforts to include the development of virus-like particle formulations derived from a novel yeast platform. (scitechnol.com)
  • Poxvirus-based strategies for combined vaccine and tumor microenvironment manipulation In: Gene Therapy of Cancer: Translational Approaches from Preclinical Studies to Clinical Implementation. (cinj.org)
  • A new possible way of treating prostate cancer is using a vaccine that may help stimulate the immune system. (survivornet.com)
  • Researchers want to see whether this vaccine, given with enzalutamide, is more effective at treating advanced prostate cancer than enzalutamide alone. (survivornet.com)
  • To compare the safety and effectiveness of enzalutamide with and without vaccine therapy for advanced prostate cancer. (survivornet.com)
  • PROSTVAC is a therapeutic cancer vaccine which is designed to induce an anti-tumor immune response. (survivornet.com)
  • Specifically, our group has shown that enzalutamide can increase thymic production of na(SqrRoot) ve T-cells, which could be activated by a cancer vaccine. (survivornet.com)
  • B16F10-bearing mice were preconditioned with 5Gy TBI and given a tripartite ACT therapy (consisting of transferred pmel-1 CD8 + T cells, vaccination with fowlpox encoding gp100, and IL-2) along with TLR4 agonist LPS. (biomedcentral.com)
  • Oncolytic virotherapy for cancer treatment utilizes naturally occurring or engineered viruses for selective infection and cancer cell death without any adverse effect on normal cells. (oncotarget.com)
  • In order to better understand differences in the outcome of infectious bursal disease virus (IBDV) infection, we inoculated a very virulent (vv) strain into White Leghorn chickens of inbred line W that was previously reported to experience over 24% flock mortality, and three inbred lines (15I, C.B4 and 0) that were previously reported to display no mortality. (imperial.ac.uk)
  • Quantitative proteomics analysis revealed compromised chicken dendritic cells function at early stage of very virulent infectious Bursal disease virus infection. (upm.edu.my)
  • Whooping cough is caused by________: A. Streptococcus Bacillus B. Pertussis Bacillus C. Vericella virus D. Non-specific viral infection 20. (pdfcoffee.com)
  • Vaccines can be prophylactic (to prevent or ameliorate the effects of a future infection by a natural or "wild" pathogen ), or therapeutic (to fight a disease that has already occurred, such as cancer ). (webot.org)
  • RNA viruses may escape acquired humoral and cellular immune responses by mutations in protective antigenic epitopes (e.g., avian influenza viruses), while accessory nonstructural proteins or multifunctional structural proteins interfere with the interferon system (e.g. (imperial.ac.uk)
  • 1 National Cancer Institute (NCI) and 2 National Institutes of Allergy and Infectious Diseases (NIAID), NIH, Bethesda, Maryland, USA. (jci.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Induction of CD8+ cytotoxic T cells is considered one of the important correlates for the protective efficacy of candidate human immunodeficiency virus type 1 (HIV-1) vaccines. (lookformedical.com)
  • By understanding how these viruses resist antiviral environments, more effective rationally designed therapeutics and live-attenuated vaccines can be produced. (scitechnol.com)
  • In human colon cancer xenograft model, a single intratumoral inoculation of the recombinant Raccoonpox virus, in combination with 5-fluorocytosine administration, produced relevant tumor growth control. (oncotarget.com)
  • The virus causes a lymphoid cancer (i.e., like lymphoma in humans) but, because chickens do not have lymph nodes, the cancer cells localise in the nerves (causing weakness and paralysis), the eyes (causing an eye colour change), the skin (causing nodules), and the liver and ovary (causing cancerous growths). (rspca.org.au)
  • 1%) metastatic non-small cell lung cancer without EGFR or ALK alterations, and in combination with Opdivo (nivolumab) and platinum-based chemotherapy as first-line therapy in patients with metastatic or recurrent non-small cell lung cancer without EGFR or ALK alterations (FDA.gov). (jax.org)
  • Our findings have clinical implications for the design of next generation immune-based therapies for patients with cancer. (biomedcentral.com)
  • Phase III trial has demonstrated a 4.8 month improvement in survival and a 37% risk reduction in death in metastatic castration resistant prostate cancer (mCRPC) patients who have had previous docetaxel. (survivornet.com)
  • Determine if prostate-specific antigen (PSA)-TRICOM combined with enzalutamide will increase time to progression (as defined by Prostate Cancer Clinical Trials Working Group 2 criteria, incorporated in section 5.2) in chemotherapy-naive metastatic castration resistant prostate cancer patients compared to enzalutamide alone. (survivornet.com)
  • The recombinant viruses obtained were unstable, although the foreign genes were expressed efficiently in the mammalian cells infected with the viruses. (lookformedical.com)
  • Numerous tumor cell lines were infected with ten non-vaccinia poxviruses to identify which virus displayed the most potential as an oncolytic agent. (oncotarget.com)
  • Raccoonpox virus was the most potent of the tested poxviruses and was highly effective in controlling cell growth in all tumor cell lines. (oncotarget.com)
  • Herein, we used TLR4 agonist LPS as a tool to address how and when to use TLR agonists to effectively improve cancer immunotherapy. (biomedcentral.com)
  • 90% of pancreatic cancers (PC) and may be an appropriate immunotherapy target. (bmj.com)
  • Primary interests include clinical trials design and the design and analysis of biomarker and imaging studies especially in the areas of GI cancer, lymphoma, melanoma, transplant and cancer immunotherapy. (dukecancerinstitute.org)
  • To investigate further the oncolytic capacity of the Raccoonpox virus, we have generated a thymidine kinase (TK)-deleted recombinant Raccoonpox virus expressing the suicide gene FCU1 . (oncotarget.com)
  • The results demonstrated significant antitumoral activity of this new modified Raccoonpox virus armed with FCU1 and this virus could be considered to be included into the growing armamentarium of oncolytic virotherapy for cancer. (oncotarget.com)
  • Numerous preclinical and clinical trials have been performed in a variety of cancer indications using several strains of oncolytic VACV including Wyeth, Western Reserve, Lister, and Copenhagen strains [ 2 - 6 ]. (oncotarget.com)
  • DNA viruses such as herpesvirus and poxvirus have multiple genes, some of them host-derived, which interfere with effective innate or acquired immune responses. (imperial.ac.uk)
  • Immunoevasion is a common ploy by which viruses neutralize or evade immune responses. (imperial.ac.uk)
  • As a post-doctoral fellow, he developed a program to study medically importantflaviviruses (West Nile and dengue virus) and their interaction with the innate immune signaling pathway. (scitechnol.com)
  • His main focus is investigating the role of West Nile and dengue virus proteins in abrogating the response to the antiviral innate immune molecule Interferon. (scitechnol.com)
  • Identification of the canarypox virus thymidine kinase gene and insertion of foreign genes. (lookformedical.com)
  • We mapped the canarypox virus (CaPV) thymidine kinase (TK) gene within a 5.8-kbp XbaI fragment of the genome by Southern blotting using the fowlpox virus (FPV) TK gene as a probe. (lookformedical.com)
  • Pancreatic cancer (PC) is a devastating disease. (bmj.com)
  • This TK-deleted Raccoonpox virus was notably attenuated in normal primary cells but replicated efficiently in numerous tumor cell lines. (oncotarget.com)
  • Its potency to preferentially infect and kill cancer cells has been proven for many years and advanced clinical trials are ongoing to evaluate this virus in human [ 1 ]. (oncotarget.com)
  • Virus concentration was determined by the plaque titration method using BSC-1 cells. (tech-strategy.org)
  • It is given after chemotherapy and surgery to help the body destroy the cancer cells. (survivornet.com)
  • It will help white blood cells recognize and kill the cancer cells in and around the prostate. (survivornet.com)
  • We then inserted the genes encoding lacZ, luciferase (luci), and envelope of human T-lymphotropic virus type 1 (HTLV-1 env) into the TK gene of CaPV to evaluate its suitability as an expression vector. (lookformedical.com)
  • Cancer Gene Ther. (cinj.org)
  • In a randomized controlled Phase 2 trial, PROSTVAC (rilimogene galvacirepvec/rilimogene glafolivec) therapy was associated with a prolongation of survival by 8.5 months in men with metastatic castrate-resistant prostate cancer. (survivornet.com)
  • Quantitation of Vaccinia Viral Titers Two groups of mice were injected with 1 107 PFU of the VJS6 recombinant virus by tail vein injection. (tech-strategy.org)
  • One group also received murine IL-10 (1 g) by i.p. injection starting 12 hours after virus administration and then daily for 5 days. (tech-strategy.org)
  • Cell viability indicated that tumor cell lines were differentially susceptible to each virus. (oncotarget.com)
  • To induce CD8+ cytotoxic T lymphocytes (CTLs) along with neutralizing antibody and CD4+ T cell help, a live canarypox virus construct expressing gp120, transmembrane gp41, the gag and protease genes, and sequences containing CTL epitopes in nef and pol was given simultaneously with, or followed by, rgp120 SF2. (lookformedical.com)
  • In this study, we examined the feasibility of Ag transfer to cultured human DCs using the host range-restricted avipoxvirus, fowlpoxvirus (FWPV). (lookformedical.com)
  • Imaging studies will be used to monitor the cancer before treatment. (survivornet.com)
  • Evaluating cytotoxicity potency of glucomoringin from the seeds of Moringa oleifera lam against prostate cancer cell line, PC-3. (upm.edu.my)
  • Enzalutamide is a hormone therapy that is used to treat advanced prostate cancer . (survivornet.com)
  • Men at least 18 years of age who have advanced castration-resistant prostate cancer. (survivornet.com)
  • Within each experimental group, some individuals experienced more severe disease than others but line 15I birds experienced milder disease based on average clinical scores, percentage of birds with gross pathology, average bursal lesion scores and average peak bursal virus titre. (imperial.ac.uk)
  • Microbial components, such as LPS, are key mediators of total body irradiation (TBI) enhancement, but our ability to strategically use these toll-like receptor (TLR) agonists to bolster the potency of T cell-based therapies for cancer remains elusive. (biomedcentral.com)
  • Participants will continue to take the study drug for as long as the cancer does not grow and the side effects are not severe. (survivornet.com)
  • Harold L. Moses is the Ingram Professor of Cancer Research, Professor of Cancer Biology, Medicine and Pathology, and director emeritus at the Vanderbilt-Ingram Cancer Center. (academicinfluence.com)