We sequenced (NextSeq, Illumina) and performed an in-depth analysis of the genome sequences of 88 Campylobacter jejuni and 2 Campylobacter coli strains isolated from clinical samples in Chile. (unab.cl)
We identified a high genetic diversity among C. jejuni strains and the emergence of prevalent clonal complexes, which were not identified in our previous reports. (unab.cl)
While ~40% of strains harbored a mutation in the gyrA gene associated with fluoroquinolone resistance, no macrolide-resistance determinants were detected. (unab.cl)
The antibiotic sensitivity and the serotype and molecular type (MT) distribution of 41 Campylobacter jejuni strains isolated from individual patients in Tawam Hospital, Al Ain, United Arab Emirates, were investigated. (uaeu.ac.ae)
All resistant strains carried the Thr-86 to He mutation in the gyrase A (gyrA) gene, as shown by mismatch amplification mutation assay (MAMA) and confirmed by sequencing. (uaeu.ac.ae)
Eight of the 35 ciprofloxacin-resistant strains, isolated over a period of more than 1 year, represented the largest MT, also carrying the same allelic variant of the gyrA gene. (uaeu.ac.ae)
Virulence genotype analysis revealed that strain CR1 lacked hemolytic phospholipase C and D, three genes for LPS biosynthesis and had reduced antibiotic resistance genes when compared with clinical strains. (frontiersin.org)
PCR showed that 57 isolates carried eae and bfp genes (typical EPEC strains), and 14 possessed only the eae gene (atypical EPEC strains). (microbiologyresearch.org)
The authors have identified two novel intimin genes ( μ B and ξ R/ β 2B) in typical EPEC strains of serotypes O55 : H51/H− and O119 : H6/H−. The complete nucleotide sequences of the novel μ B and ξ R/ β 2 variant genes were determined. (microbiologyresearch.org)
Fluoroquinolone resistance1
These results show that the local incidence of fluoroquinolone resistance among C. jejuni is one of the highest reported worldwide. (uaeu.ac.ae)
To understand the prevalence and genetic characteristics of Campylobacter in Chinese local dual-purpose (meat and eggs) chickens, the genomes of 30 Campylobacter isolates, including 13 C. jejuni and 17 C. coli from Jianghan-chickens in central China, were sequenced and tested for antibiotic susceptibility. (figshare.com)
The results showed that CC-354 and CC-828 were the dominant clonal complexes of C. jejuni and C. coli, respectively, and a phylogenetic analysis showed that three unclassified multilocus sequence types of C. coli were more closely genetically related to C. jejuni than to other C. coli in this study. (figshare.com)
The antibiotic resistance rate of C. coli was higher than that of C. jejuni. (figshare.com)
Antibiotic-resistant2
Food-borne antibiotic-resistant Campylobacter poses a serious threat to public health. (figshare.com)
It was also demonstrated that stable MTs could persist for a relatively long time among the clonally unrelated antibiotic-resistant isolates of C. jejuni. (uaeu.ac.ae)
Isolates carried1
Based on the partial sequences of gyrA, resistant isolates carried 10 distinct alleles, eight of them representing new variants. (uaeu.ac.ae)
Sequences1
Using phylogenetic analysis of partial fusion gene sequences, these viruses clustered with recent European PPMV-1 isolates (EU/re) within the genotype VIb/1. (bioone.org)
Antimicrobial1
The aims of this work were to determine genetic diversity, presence of antimicrobial resistance determinants and virulence potential of Campylobacter spp. (unab.cl)
Mutation2
The GyrA T86I mutation and 15 acquired resistance genes were detected with whole-genome sequencing (WGS). (figshare.com)
The ciprofloxacin and tetracycline resistance phenotypes correlated strongly with the GyrA T86I mutation and tet(O)/tet(L), respectively, but for other antibiotics, the correlation between genes and resistance phenotypes were weak, indicating that there may be resistance mechanisms other than the resistance genes detected in this study. (figshare.com)
Resistance genes1
In almost all cases ESBL and quinolone resistance genes were located on the same plasmid. (afpm.org.my)
Whole-genome1
Metagenomic approaches in microbial ecology: an update on whole-genome and marker gene sequencing analyses. (cdc.gov)
Virulence2
Interestingly, gene clusters encoding virulence factors such as the T6SS or genes associated with long-term sequelae such as Guillain-Barré syndrome showed lineage-relatedness. (unab.cl)
Virulence gene analysis showed that several genes related to adhesion, colonization, and invasion (including cadF, porA, ciaB, and jlpA) and cytolethal distending toxin (cdtABC) were only present in C. jejuni. (figshare.com)
Bacterial2
Campylobacter is the leading cause of bacterial gastroenteritis worldwide and an emerging and neglected pathogen in South America. (unab.cl)
Genes belonging to proteases, bacterial exporters and DNA stabilization were found to be under strong positive selection, thus facilitating pathogenicity and survival of the outliers. (frontiersin.org)
Analysis2
Analysis included identification by MALDI-TOF mass spectrometry, susceptibility testing, XbaI-PFGE, microarray, PCR and sequencing of specific ESBL genes, PCR based replicon typing (PBRT) of plasmids and Southern blot hybridization. (afpm.org.my)
Array analysis revealed 18 isolates with ESBL genes and one isolate with solely non-ESBL beta-lactamase genes. (afpm.org.my)
Overall, this study extends our knowledge of the epidemiology and antibiotic resistance of Campylobacter in local Chinese dual-purpose chickens. (figshare.com)
Region1
In addition, the quinolone resistance genotype was characterized by screening for plasmid mediated quinolone resistance (PMQR) genes and mutations in the quinolone resistance determining region (QRDR) of gyrA and parC. (afpm.org.my)
Presence1
1). EPEC isolates were examined for the presence of eaeA , bfpA and stx genes by PCR. (microbiologyresearch.org)
Isolates6
Six nalidixic acid-resistant isolates of C. jejuni were shown to carry mutations in gyrA. (nih.gov)
Gibreel A., Skold O., High-level resistance to trimethoprim in clinical isolates of Campylobacter jejuni by acquisition of foreign genes (dfr1 and dfr9) expressing drug-insensitive dihydrofolate reductases, Antimicrob. (vetres.org)
Gibreel A., Skold O., An integron cassette carrying dfr1 with 90-bp repeat sequences located on the chromosome of trimethoprim-resistant isolates of Campylobacter jejuni , Microb. (vetres.org)
To understand S. typhimurium's epidemiological characteristics in China, multi-locus sequence typing, biofilm-forming ability, antimicrobial susceptibility testing, and resistant genes of isolates from different regions and sources (human, food) were investigated. (bvsalud.org)
Dendrogram based on the hierarchic numerical analysis of the RAPD-PCR profiles obtained for 20 Campylobacter isolates, employing the Pearson correlation coefficient and UPGMA for clustering. (biomedcentral.com)
We found that Escherichia coli isolates were resistant against tilmicosin, tetracycline, ampicillin, amoxicillin with clavulanic acid, ticarcillin, and cephalothin, and contained genes considered responsible for conferring tetracycline resistance. (bioone.org)
Gibreel A., Sjogren E., Kaijser B., Wretlind B., Skold O., Rapid emergence of high-level resistance to quinolones in Campylobacter jejuni associated with mutational changes in gyrA and parC , Antimicrob. (vetres.org)
Fluoroquinolone1
Human Health Impact of Fluoroquinolone Resistant Campylobacter Attributed to the Consumption of Chicken. (nih.gov)
Salmonella2
Results of this study suggest that the BolA gene may serve as a potential target of therapeutic or preventative strategies to control Salmonella Typhimurium infections. (bvsalud.org)
It has been associated with gastrointestinal (GI) infections with Shigella, Salmonella, Campylobacter, and other organisms, as well as with genitourinary (GU) infections (especially with Chlamydia trachomatis). (medscape.com)
Aarestrup F.M., Agerso Y., Gerner-Smidt P., Madsen M., Jensen L.B., Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers, and pigs in Denmark, Diagn. (vetres.org)
Bottcher I., Jacob J., The occurrence of high-level streptothricin resistance in thermotolerant campylobacters isolated from the slurry of swine and the environment, Zentralbl. (vetres.org)
Derbise A., Aubert S., El Solh N., Mapping the regions carrying the three contiguous antibiotic resistance genes aadE , sat4 , and aphA-3 in the genomes of staphylococci, Antimicrob. (vetres.org)
Endtz H.P., Ruijs G.J., van Klingeren B., Jansen W.H., van der Reyden T., Mouton R.P., Quinolone resistance in Campylobacter isolated from man and poultry following the introduction of fluoroquinolones in veterinary medicine, J. Antimicrob. (vetres.org)
Aarestrup F.M., Nielsen E.M., Madsen M., Engberg J., Antimicrobial susceptibility patterns of thermophilic Campylobacter spp. (vetres.org)
Engberg J., Andersen S., Skov R., Aarestrup F.M., Gerner-Smidt P., Comparison of two agar dilution methods and three agar diffusion methods including the Etest for antibiotic susceptibility testing of thermophilic Campylobacter species, Clin. (vetres.org)
Encodes1
The gyrA gene of Campylobacter jejuni UA580, which encodes the A subunit of DNA gyrase, was cloned and its nucleotide sequence was determined. (nih.gov)
Sequence2
Both the nucleotide sequence and the putative amino acid sequence show ca. 50% identity with those of other gyrA genes from gram-positive and gram-negative bacteria. (nih.gov)
Furthermore, the sequence analysis of the gag gene region detected the existence of the A genotype of small ruminant lentiviruses in sheep. (researcher.life)
Update1
Metagenomic approaches in microbial ecology: an update on whole-genome and marker gene sequencing analyses. (cdc.gov)
Ability2
269BolA+ showed 3.6-fold and 5.2-fold higher biofilm formation ability than WT269 and 269ΔBolA, respectively, by upregulation biofilm formation-related genes. (bvsalud.org)
In Caco-2 and HeLa cells, 269â ³BolA showed 2.8- and 3-fold lower cell adhesion ability, respectively, and 4- and 2-fold lower cell invasion ability, respectively, than WT269, through downregulation of the virulence genes. (bvsalud.org)