Camp protein. Medical search. Frequent questions

Phosphorylation of the cAMP response element binding protein (pCREB) triggered by associative learning was monitored immunohistochemically in different areas of the mouse brain during a 6-h interval, starting immediately after training. (elsevierpure.com)
cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. (nih.gov)
Pharmacological inhibition of p38 mitogen-activated protein kinase (p38-MAPK) inhibited TNF-α-induced CREB phosphorylation. (elsevierpure.com)
In conjunction with information presented in the following paper in this issue of this journal, the results substantiate the hypothesis that PP‐1 activity towards the glycogen‐metabolising enzymes is regulated in vivo by reversible phosphorylation of a targetting subunit (G) that directs the C subunit to glycogen-protein particles. (dundee.ac.uk)
Here DARPP-32 phosphorylation by protein kinase A (PKA), DARPP-32 into a powerful protein phosphatase 1 (PP1) inhibitor. (pp1a.com)
HSL from chicken adipose tissue exhibits remarkable activation upon phosphorylation with cAMP-dependent protein kinase (cAMP-PK) compared to HSL from rat and human adipose tissue. (lu.se)

The use of amino acids, proteins and peptides is not considered to have any environmental impact. (janusinfo.se)
According to the European Medicines Agency guideline on environmental risk assessments for pharmaceuticals (EMA/CMP/SWP/4447/00) vitamins, electrolytes, amino acids, peptides, proteins, carbohydrates, lipids, vaccines and herbal medicinal products are exempted because they are unlikely to result in significant risk to the environment. (janusinfo.se)

cAMP-dependent protein kinase A (PKA) is a ubiquitously expressed serine/threonine kinase that regulates a variety of cellular functions. (mdc-berlin.de)
The glycogen‐associated form of protein phosphatase‐1 (PP‐1 G ) is a heterodimer comprising a 37‐kDa catalytic (C) subunit and a 161‐kDa glycogen‐binding (G) subunit, the latter being phosphorylated by cAMP‐dependent protein kinase at two serine residues (site 1 and site 2). (dundee.ac.uk)

In Escherichia coli, cyclic AMP receptor protein (CRP) can regulate the transcription of more than 100 genes. (wikipedia.org)
Cyclic AMP receptor protein was originally identified as a catabolite gene activator protein. (bvsalud.org)

Regulation of protein phosphatase‐1G from rabbit/skeletal muscle: 1. (dundee.ac.uk)
The efficient dephosphorylation of site 2 by the Ca 2+ /calmodulin‐stimulated protein phosphatase (2B) provides a potential mechanism for regulating PP‐1 activity in response to Ca 2+ , and represents an example of a protein phosphatase cascade. (dundee.ac.uk)
This study found an increase in excess GR transcriptional activity of protein phosphatase 1 alpha (PP1α) in HEK-293 cells and a decrease in the expression levels of GR-responsive gene knockdown following PP1α model A549 cells endogenously . (pp1a.com)

New England Biolabs offers proteases, phosphatases, kinases, chitinases and protein markers and ladders, as well as tools for phage display and protein labeling. (neb.com)
Cell surface cyclic AMP receptors are not included (CYCLIC AMP RECEPTORS), nor are the eukaryotic cytoplasmic cyclic AMP receptor proteins, which are the regulatory subunits of CYCLIC AMP-DEPENDENT PROTEIN KINASES. (bvsalud.org)

Mouse monoclonal to cAMP Protein Kinase Catalytic subunit - Store at -20°C or lower. (extinct-website.com)

cAMP-response element-binding protein (CREB) is the stimulus-induced transcription factor and activates transcription of target genes such as c-fos and interleukin-6. (elsevierpure.com)
the ≃45-kDa protein may represent CREB. (elsevierpure.com)
These results indicate that CREB/ATF-1-related factors act coordinately with the cell-specific factor GHF1 to mediate cAMP-dependent regulation of hGH-1 gene transcription in anterior pituitary somatotrophs. (elsevierpure.com)

also known as catabolite activator protein, CAP) is a regulatory protein in bacteria. (wikipedia.org)

Using EPAC1 (exchange protein directly activated by cAMP 1) as bait in two-hybrid screens of foetal and adult human brain libraries, we identified the LC2 (light chain 2) of MAP1A (microtubule-associated protein 1A) as a protein capable of interaction with EPAC1. (hw.ac.uk)

CRP protein binds cAMP, which causes a conformational change that allows CRP to bind tightly to a specific DNA site in the promoters of the genes it controls. (wikipedia.org)
A transcription factor that binds to DNA sequences called cAMP response elements (CRE) and increases the transcription of certain genes . (emf-portal.org)
This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. (nih.gov)
The known genetic causes are mutations of the genes CREBBP (OMIM 600140, cAMP-response-element binding protein-BP ), located at chromosome 16p13.3 and EP300 (OMIM 602700, E1A-binding protein ), a CREBBP homolog, located at chromosome 22q13.2. (biomedcentral.com)
Analysis of the intact hGH gene or hGH 5'- flanking DNA (5'-FR) coupled to the hGH cDNA or chloramphenicol acetyltransferase or luciferase genes, indicated that cAMP primarily stimulated hGH promoter activity. (elsevierpure.com)
False positive non-synonymous polymorphisms of G-protein coupled receptor genes. (nih.gov)

However, it is unknown whether cAMP protects against DILI and, if so, which downstream targets of cAMP are implicated in the protective mechanism including the classical protein kinase A (PKA) pathway or alternative pathways like the exchange protein directly activated by cAMP (EPAC). (rug.nl)

Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
Interestingly, recombinant protein of wild-type PDE10A2, but not PDE10A2 mutant with an Ala replacement at Thr 16 , was distributed to cytosolic fractions by co-transfection with a plasmid encoding the catalytic subunit of PKA. (johnshopkins.edu)

The generally held view of these processes is that neurotransmitter-induced modulation of the most widely expressed Ca2+ channels in these cells (N-, P/Q- and L-type) follows two distinct pathways: a direct membrane-delimited G(i/o)-protein-induced inhibition of N- and P/Q-type and a remote cAMP-mediated facilitation of L-channels. (unito.it)
maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. (rcsb.org)
Akt pathways have effects on apoptosis, protein synthesis, metabolism and cell cycle. (springer.com)
AMPK pathways inhibit biosynthetic pathways with energy consumption, such as protein, fatty acid and glycogen synthesis. (springer.com)

Treatment of the pertussis toxin-treated cells with cholera toxin before CB1 receptor activation amplified the stimulatory pathway, suggesting that this response was mediated through a G s -type G-protein. (jneurosci.org)

Unlike N- and P/Q-channels, however, the inhibition of L-channels is largely independent of voltage and develops in parallel with cAMP-mediated potentiation of channel gating. (unito.it)
In ventricular myocytes, NO can increase cAMP levels through a cGMP-dependent inhibition of PDE3. (ox.ac.uk)
Pertussis toxin treatment of striatal neurons prevented the inhibition of cAMP accumulation by D2 receptors but unmasked a cannabinoid receptor-mediated stimulatory effect on cAMP accumulation. (jneurosci.org)

You lose a lot of carbohydrates, fluid and protein. (stack.com)

Platelet activation allows binding of these proteins, which bridges adjacent platelets. (medscape.com)
The alpha granules contain hemostatic proteins such as fibrinogen, vWf, and growth factors (eg, platelet-derived growth factor and transforming growth factors). (medscape.com)

Stay connected and learn about new exclusive content, giveaways, and news from Burn Boot Camp. (burnbootcamp.com)

sand flies collected from refugee camps in Greece, indicating increased risk of infection among local populations. (cdc.gov)
A vectorborne pathogen surveillance network targeting refugee camps was deployed in Greece during June-September 2017. (cdc.gov)
We surveyed 4 refugee camps in Greece in 2017: the Lagadikia and Diavata camps in the Thessaloniki Regional Unit, northern Greece, and the Vial and Souda camps on the island of Chios in the Northeastern Aegean Islands complex. (cdc.gov)
There are 27 refugee camps in the occupied Palestinian territory (19 in the West Bank and eight in the Gaza Strip). (who.int)

First, elevated cAMP levels protect against diclofenac-induced apoptosis in primary hepatocytes via maintenance of mitochondrial integrity. (rug.nl)

The ginseng-treated rats exhibited significantly increased sperm count and motility with enhanced levels of CREM messenger RNA and protein. (nih.gov)

A total protein and A/G ratio test is often included as part of a comprehensive metabolic panel , a test that measures proteins and other substances in the blood. (medlineplus.gov)

Cannabinoids exert many of their effects through activation of G i -protein-coupled receptors. (jneurosci.org)
Both D1 and D2 dopamine receptors also couple to adenylate cyclase via G-proteins. (jneurosci.org)
whereas dopamine D2 receptors inhibit adenylate cyclase via G i -proteins ( Sibley and Monsma, 1992 ). (jneurosci.org)
Previously, CB1 receptors have been shown to inhibit D1 receptor-mediated cAMP accumulation ( Bidaut-Russell and Howlett, 1991 ). (jneurosci.org)

Dopamine and cyclic-AMP activated Mr32kDa phosphoprotein (DARPP-32) is a central signaling proteins in neurotransmission. (pp1a.com)

Together with our two-hybrid results, this suggests that the cAMP-binding domain of EPAC1 mediates interaction with LC2. (hw.ac.uk)
Collectively, these data demonstrate that PDE4B mediates ERK-dependent up-regulation of mucin MUC5AC by S. pneumoniae by inhibiting cAMP-PKA-dependent MKP-1 pathway. (ewha.ac.kr)

At "Class I" CRP-dependent promoters, CRP binds to a DNA site located upstream of core promoter elements and activates transcription through protein-protein interactions between "activating region 1" of CRP and the C-terminal domain of RNA polymerase alpha subunit. (wikipedia.org)
At "Class II" CRP-dependent promoters, CRP binds to a DNA site that overlaps the promoter -35 element and activates transcription through two sets of protein-protein interactions: (1) an interaction between "activating region 1" of CRP and the C-terminal domain of RNA polymerase alpha subunit, and (2) an interaction between "activating region 2" of CRP and the N-terminal domain of RNA polymerase alpha subunit. (wikipedia.org)
At "Class III" CRP-dependent promoters, CRP functions together with one or more "co-activator" proteins. (wikipedia.org)
At most CRP-dependent promoters, CRP activates transcription primarily or exclusively through a "recruitment" mechanism, in which protein-protein interactions between CRP and RNA polymerase assist binding of RNA polymerase to the promoter. (wikipedia.org)

Binding of cAMP to CRP leads to a long-distance signal transduction from the N-terminal cAMP-binding domain to the C-terminal domain of the protein, which is responsible for interaction with specific sequences of DNA. (wikipedia.org)
Stanciu, M , Radulovic, J & Spiess, J 2001, ' Phosphorylated cAMP response element binding protein in the mouse brain after fear conditioning: Relationship to Fos production ', Molecular Brain Research , vol. 94, no. 1-2, pp. 15-24. (elsevierpure.com)
Removal of the cAMP-binding domain of EPAC1 (DeltacAMP-EPAC1) appeared to disrupt targeting of EPAC1 in cells resulting in a more dispersed staining pattern. (hw.ac.uk)
We found that deletion of the cAMP-binding domain inhibited interaction between EPAC1 and LC2 in a two-hybrid assay, but removal of the DEP domain had little effect. (hw.ac.uk)
LC2 was found to interact with a glutathione-S-transferase-fusion protein of the cAMP-binding domain of EPAC1 in a pull-down assay, but not the DEP, REM (Ras exchange motif) or CAT (catalytic) domains. (hw.ac.uk)
pCRE) and a pituitary-specific transcription factor (GHF1/Pit1) binding site at nucleotides -123/-112 (dGHF1) which were required for cAMP responsiveness. (elsevierpure.com)
Shepard, AR, Zhang, W & Eberhardt, NL 1994, ' Two CGTCA motifs and a GHF1/Pit1 binding site mediate cAMP-dependent protein kinase A regulation of human growth hormone gene expression in rat anterior pituitary GC cells ', Journal of Biological Chemistry , vol. 269, no. 3, pp. 1804-1814. (elsevierpure.com)

That way, more protein will go toward building muscle, repairing injured tissues and keeping your immune system strong. (stack.com)

cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. (nih.gov)

FRET is a biophysical method that can assess the presence of two adjacent proteins (usually within 10 nm of each other) by measuring energy transfer between two fluorophores. (aacrjournals.org)

We applied an immunoprecipitation assay to demonstrate protein interaction between EPAC1 and LC2 in co-transfected human embryonic kidney 293 cells. (hw.ac.uk)

CRP then activates transcription through direct protein-protein interactions with RNA polymerase. (wikipedia.org)
These tend to fall roughly into two categories: validation of putative or suspected interactions and identification of unknown associated proteins. (aacrjournals.org)
The classic method for analysis of protein-protein interactions is the co-immunoprecipitation (co-IP), also known as a "pull down" ( 1, 2 ), which constitutes the gold standard in the field. (aacrjournals.org)
Unlike immunofluorescence microscopy approaches, which provide correlative information about proteins sharing cellular space, co-IP can confirm direct often high-affinity physical interactions between two proteins or protein complexes of equal or better affinity. (aacrjournals.org)
The validity of co-IP approaches can quickly deteriorate when forced expression of tagged proteins are used to investigate possible interactions, yet this approach, particularly when endogenous factors are assessed, can provide useful information about potential protein associations. (aacrjournals.org)

The National Communicable Disease Center (NCDC) helped to treat the 500,000 cases of malnutrition, by setting up food distribution centers and clinics in refugee relief camps, surrounding the Nigerian war zone. (cdc.gov)
Protein enriched foods, including dried codfish and powdered milk, were made available to the children suffering with the protein-calorie malnutrition disease called kwashiorkor. (cdc.gov)
4. Protein-energy malnutrition is increasing on the African continent whereas it is decreasing in other regions of the world. (who.int)
Thus, protein-energy malnutrition is increasing in Africa5 whereas it is decreasing in other regions of the world. (who.int)

This stimulation of cAMP was not Ca 2+ -sensitive and was unaffected by a range of protein kinase inhibitors. (jneurosci.org)
Stimulation of cAMP accumulation was not observed after pertussis toxin treatment of CHO cells expressing the human CB2 receptor, suggesting that this novel signaling pathway is unique to the cannabinoid CB1 receptor. (jneurosci.org)

The effect of Korean ginseng (ginseng) on spermatogenesis and cAMP-responsive element modulator (CREM) in rat testes was evaluated using sperm analysis, reverse transcription polymerase chain reaction, and Western blot analysis. (nih.gov)

Cyclic GMP can directly stimulate If but it is ∼10-fold less potent than the 'physiological' If-gating nucleotide, cAMP. (ox.ac.uk)

Whether your goal is fat loss, muscle mass gain or healthier bones, getting enough protein is critical. (gumsaba.com)
If you do not get enough protein, you may not realize the full performance benefits of your training. (stack.com)

This pack is great for adding to eggs or ramen to provide additional calories and protein for your hike or climb. (backcountry.com)

The number of studies that have used protein interaction discovery approaches for identification of associated factors is overwhelming. (aacrjournals.org)

3. In Africa, nutritional problems in infants and young children occur in a context characterized by factors such as the prevalent low birth weight, household food insecurity, inappropriate infant and young child feeding practices, including the absence of food safety and hygiene, and inadequate intake of proteins and energy foods. (who.int)

We established the cis-acting elements which mediate cAMP responsiveness of the human growth hormone (hGH) gene in transiently transfected rat anterior pituitary tumor GC cells. (elsevierpure.com)

The cAMP-elevating agent forskolin, the pan-phosphodiesterase inhibitor IBMX and EPAC inhibitors CE3F4 and ESI-O5 were used to assess the role of cAMP and its effectors, PKA or EPAC. (rug.nl)

Gel shift analyses indicated that similar, ubiquitous, thermostable protein(s) specifically bound the pCRE and dCRE motifs. (elsevierpure.com)
We do not aim to provide an exhaustive analysis of all available techniques, but instead will highlight some of the most widely used methods for protein-protein assessment and will describe some recent advances in global protein-protein analyses that are enabling non-experts to identify interactomes of their favorite factor. (aacrjournals.org)

Cannabinoids act at the CB1 receptor to inhibit adenylate cyclase activity via a pertussis toxin-sensitive G-protein. (jneurosci.org)

There are numerous methods for the discovery of unknown associated protein networks, with purification of the bait (the protein of interest) followed by mass spectrometry as a common theme. (aacrjournals.org)

It encodes a membrane-associated protein called the cystic fibrosis transmembrane conductance regulator (CFTR). (msdmanuals.com)

cAMP is a signaling molecule important for a variety of cellular functions. (nih.gov)
This involves purification of a specific protein from the pool of cellular proteins, followed by Western blotting of a suspected interacting protein ( 1 ). (aacrjournals.org)

Conclusion: cAMP elevation protects hepatocytes against diclofenac-induced cell death, a process primarily involving EPACs. (rug.nl)

EcN was armed with HlyE, a small cytotoxic protein, under the control of the araBAD promoter (P BAD ). (nature.com)
G-protein-coupled receptor GPR161 is overexpressed in breast cancer and is a promoter of cell proliferation and invasion. (nih.gov)

Overall, this study proposes a new strategy for the bacteria-mediated delivery of therapeutic proteins to tumors. (nature.com)

Aim: Investigate whether cAMP and/or its downstream targets protect against diclofenac-induced injury in hepatocytes. (rug.nl)

It is hypothesized that cAMP signaling in the Golgi area and the cytosol in neurons is controlled through alteration of subcellular localization of PDE10A brought by activation of PKA in response to intracellular elevations of cAMP. (johnshopkins.edu)
cAMP-dependent protein kinase activation decreases cytokine release in bronchial epithelial cells. (cdc.gov)

Anatomy2

Organisms2

Chemicals and Drugs31

Analytical, Diagnostic and Therapeutic Techniques and Equipment3

Phenomena and Processes7

Anthropology, Education, Sociology and Social Phenomena2

Information Science1

Phosphorylation6

Peptides2

Serine2

Receptor protein2

Phosphatase3

Kinases2

Kinase catalytic1

CREB3

Catabolite activat1

Microtubule-Associa1

Genes6

Exchange protein directly activated by cAMP1

Recombinant protein3

Pathways4

Response was mediated1

Inhibition3

Carbohydrates1

Platelet2

Boot Camp1

Refugee camps4

Apoptosis1

Rats1

Substances1

Receptors4

Dopamine1

Mediates2

Promoters4

Binding7

Tissues1

Regulatory1

Adjacent1

EPAC11

Interactions5

Malnutrition4

Stimulation2

Transcription1

Nucleotide1

Enough protein2

Calories1

Interaction1

Intake1

Tumor1

Inhibitors1

Analyses2

Inhibit1

Bait1

Encodes1

Cellular2

Primarily1

Promoter2

Bacteria1

Investigate1

Activation2