• Phosphorylation of calmodulin. (wikipedia.org)
  • In the present study, we examined the role of protein tyrosine phosphorylation and the mitogen-activated protein kinases, ERK1/2,in mediating BK-induced AP-1 and DNA replication in cultured rat mesangial cells. (nih.gov)
  • BK (10(-9) to 10(-7) M) stimulated a rapid increase in tyrosine phosphorylation of multiple proteins with an estimated molecular mass of 120-130, 90-95, and 44-42 kDa. (nih.gov)
  • 0.05) in phosphorylation of Elk-1, a transcription factor required for growth factor-induced c-fos transcription. (nih.gov)
  • In accord with the stimulation of Elk-1 phosphorylation, BK induced c-fos gene expression and the production of Fos/AP-1 complexes. (nih.gov)
  • AP-1 pathway and that BK mitogenic signaling is critically dependent on protein tyrosine phosphorylation. (nih.gov)
  • Carbon disulfide inhalation increases Ca2+/calmodulin-dependent kinase phosphorylation of cytoskeletal proteins in the rat central nervous system. (cdc.gov)
  • Calmodulin 1 is a protein that in humans is encoded by the CALM1 gene. (wikipedia.org)
  • RESULTS: Sixteen calcium signal pathway-related proteins were identified, however, only Calmodulin-1(CALM1) was continuously increased. (bvsalud.org)
  • A new monoclonal antibody, A3B10, specific for astrocyte-lineage cells recognizes calmodulin-regulated spectrin-associated protein 1 (Camsap1). (cyphergenomics.com)
  • As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. (thermofisher.com)
  • CAMKII contains an N-terminal catalytic domain which binds ATP and substrate protein, regulatory domain (CBD) and association domain (ASD). (proteopedia.org)
  • The two lobes of CaM both compete for binding at the same site on ER-α (residues 292, 296, 299, 302, and 303), which explains why full-length CaM binds two molecules of ER-α in a 1:2 complex and stabilizes ER-α dimerization. (rcsb.org)
  • Group A Streptococcus (GAS), a β-haemolytic human pathogen, expresses a NEAT protein, Shr, which binds several haemoproteins and extracellular matrix (ECM) components. (tcdb.org)
  • Calcium binds to and regulates the small regulatory protein calmodulin that in turn binds to and regulates several hundred calmodulin binding proteins. (biomedcentral.com)
  • The Calcium Hypothesis was thus extended as the Calmodulin Hypothesis since CaM not only binds to and regulates CaMBPs critical to learning and memory but also proteins involved in the formation of amyloid plaques and tangles, hallmarks of AD [ 41 ]. (biomedcentral.com)
  • Calmodulin is an essential Ca2+-binding protein that binds to a variety of targets that carry out critical signaling functions. (lu.se)
  • This gene provides instructions for making a protein called calcium/calmodulin-dependent serine protein kinase (CASK). (medlineplus.gov)
  • Among its diverse biological actions, the vasoactive peptide bradykinin (BK) induces the transcription factor AP-1 and proliferation of mesangial cells (S. S. El-Dahr, S. Dipp, I. V. Yosipiv, and W. H. Baricos. (nih.gov)
  • the addition of 10 µM of CaM-peptide significantly decreases the interaction of htt-exon1-44Q and CaM but not the binding between CaM and calcineurin, another CaM-binding protein. (ku.edu)
  • Description: A competitive ELISA for quantitative measurement of Human Calmodulin like protein 5(CALML5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (glideruniversity.org)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Calmodulin Like Protein 5 (CALML5) in Tissue homogenates, cell lysates and other biological fluids. (glideruniversity.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Calmodulin Like Protein 5 (CALML5) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (glideruniversity.org)
  • Calmodulin variant E140G associated with long QT syndrome impairs CaMKIIδ autophosphorylation and L-type calcium channel inactivation. (nih.gov)
  • Rellos P, Pike AC, Niesen FH, Salah E, Lee WH, von Delft F, Knapp S. Structure of the CaMKIIdelta/calmodulin complex reveals the molecular mechanism of CaMKII kinase activation. (proteopedia.org)
  • For this purpose, WIN 55,212-2 was injected in pregnant wistar rats from gestation day 5 to 20 and a detailed analysis of the levels of the neurotrophin brain-derived neurotrophic factor (BDNF) as well as of the signaling molecules extracellular signal-regulated kinase (ERK)1/2 and alpha-calcium/calmodulin-dependent protein kinase II (alpha-CaMKII) was carried out in adult offspring. (researchgate.net)
  • The main aim is to identify putative protein markers to be used as putative indices of fertility. (rsc.org)
  • A total of 140 putative Ca2+-calmodulin-binding proteins were identified of which 87 proteins contained calmodulin-binding motifs. (lu.se)
  • Ca2+/Calmodulin dependent protein kinase (CaMK) are mammalian calmodulin-dependent calcium-dependent protein kinases activated by elevation of Ca+2 and calmodulin concentration to phosphorylate Ser and Thr. (proteopedia.org)
  • Thus our studies support an unusual, extended 1:2 binding mode of CaM to Orai1-CMBDs, and quantify the affinity of Orai1 for CaM. (rcsb.org)
  • 140 mouse brain proteins identified by Ca2+-calmodulin affinity chromatography and tandem mass spectrometry. (lu.se)
  • We describe the proteomic characterization of mouse brain Ca2+-calmodulin-binding proteins that were purified using calmodulin affinity chromatography. (lu.se)
  • Proteins in the eluates from four different affinity chromatography experiments were identified by 1-DE and in-gel digestion followed by LC-MS/MS. Parallel experiments were performed using two related control-proteins belonging to the EF-hand family. (lu.se)
  • The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. (cipsm.de)
  • Calcium-induced activation of calmodulin regulates and modulates the function of cardiac ion channels. (nih.gov)
  • In contrast, protein kinase C inhibition or depletion had no effect on BK-induced c-fos mRNA, AP-1-DNA binding activity, or DNA synthesis. (nih.gov)
  • This is followed by a fast Ca(2+)·calmodulin (CaM)-dependent inhibition, resulting from CaM binding to an Orai1 region called the calmodulin binding domain (CMBD). (rcsb.org)
  • 1983. Correlation of metal toxicity with in vitro calmodulin inhibition. (cdc.gov)
  • Here we demonstrate that PSRP1 is not a bona fide ribosomal protein, but rather a functional homologue of the Escherichia coli cold-shock protein pY. (cipsm.de)
  • This review focuses on emerging topics encompassing the functional involvement of aquaporin channel proteins (AQPs) and membrane transport systems, also allowing permeation of NO and hydrogen peroxide, a major ROS, in oxidative stress physiology and pathophysiology. (hindawi.com)
  • Analysis of calmodulin-kinase cascade by using functional proteomics. (nii.ac.jp)
  • This study provides a functional framework for studies on these previously uncharacterized proteins. (lu.se)
  • This gene encodes one of three calmodulin proteins which are members of the EF-hand calcium-binding protein family. (nih.gov)
  • Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. (thermofisher.com)
  • The small calcium-binding protein calmodulin (CaM) is a primary effector of calcium function and works in turn by binding to and regulating CaM-binding proteins (CaMBPs) [ 53 ]. (biomedcentral.com)
  • Calmodulin Interactions with Voltage-Gated Sodium Channels. (nih.gov)
  • Protein conformation is critically linked to function and often controlled by interactions with regulatory factors. (cipsm.de)
  • Huntington's disease (HD) is a neurodegenerative disease caused by mutant huntingtin protein containing an expanded polyglutamine tract, which may cause abnormal protein-protein interactions such as increased association with calmodulin (CaM). (ku.edu)
  • Western Blotting assays will be performed for the evaluation of the protein expression of eNOS, calmodulin, caveolin-1, Hsp-90, Nrf-2, catalase, superoxide dismutase and glutathione peroxidase, as well as their interactions through co-immunoprecipitation. (fapesp.br)
  • With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. (thermofisher.com)
  • The hemolytic Group A Streptococcus (GAS) possesses the Shr protein which as noted above, participates in iron acquisition by obtaining heme from host hemoglobin and delivering it to the adjacent receptor on the surface, Shp. (tcdb.org)
  • It has recently been demonstrated that prenatal exposure to the cannabinoid receptor 1 agonist (R)-(+)-[2,3-dihydro-5-methyl-3-(4-morpholinyl-methyl)pyrrolo[1,2,3-de]-1,4-benzoxazin-6-yl]-1-naphthalenylmethanone (WIN 55,212-2) produces memory deficit in adulthood, an effect associated with a reduced functionality of the glutamatergic system. (researchgate.net)
  • White blood cells, or leukocytes, are extremely dependent upon integrin receptor recognition of matrix proteins in order to exit the vasculature and resolve inflammatory events within the tissues. (upstate.edu)
  • Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations. (thermofisher.com)
  • The primary mediator of the intracellular effects of calcium is the calcium-binding regulatory protein, calmodulin. (medscape.com)
  • EF-1α is a highly conserved protein and functions in protein translation. (elsevierpure.com)
  • However, in immunoblot analysis, EF-1α was mainly extracted in the membrane and matrix fraction from the axonemal MTs by 1% Triton X-100 extraction. (elsevierpure.com)
  • We provide evidence that KRas translocation occurs through sequestration of the polybasic-prenyl motif by Ca 2+ /calmodulin (Ca 2+ /CaM) and subsequent release of KRas from the PM, in a process reminiscent of GDP dissociation inhibitor-mediated membrane recycling of Rab and Rho GTPases. (rupress.org)
  • Such a membrane-anchoring strategy has been adopted by a wide range of signaling proteins ( Fivaz and Meyer, 2003 ) with significant variations in the structure of these motifs (see Table S1). (rupress.org)
  • Shr is a complex, membrane-anchored protein, with a unique N-terminal domain (NTD) and two NEAT domains separated by a central leucine-rich repeat region. (tcdb.org)
  • Heme is then conveyed to the SiaABC proteins (TC# 3.A.1.14.10) for transport across the membrane. (tcdb.org)
  • This notion has been challenged by the discovery of new membrane transport functions, especially those exerted by aquaporins (AQPs), a family of membrane channel proteins widespread in nature [ 10 , 11 ]. (hindawi.com)
  • Orai1 is a plasma membrane protein that in its tetrameric form is responsible for calcium influx from the extracellular environment into the cytosol in response to interaction with the Ca(2+)-depletion sensor STIM1. (rcsb.org)
  • A further chapter discusses how surface membrane G-protein receptors in C. albicans and other fungi transmit external stimuli through 2 major protein kinase cascades. (cdc.gov)
  • In addition, EF-1α has been reported to interact with MTs and F-actin in several organisms. (elsevierpure.com)
  • Research suggests that the CASK protein may also interact with the protein produced from another gene, FRMD7 , to promote development of the nerves that control eye movement (the oculomotor neural network). (medlineplus.gov)
  • Among the 87 proteins that contained calmodulin-binding motifs, 48 proteins have not previously been shown to interact with calmodulin and 39 proteins were known calmodulin-binding proteins. (lu.se)
  • As bioactive compounds tend to interact with multiple proteins with various physiological effect, we aimed to identify other potential shared targets to understand the extent in which these chemicals could lead to GWI. (cdc.gov)
  • Subtype Differences in the Interaction of HIV-1 Matrix with Calmodulin: Implications for Biological Functions. (nih.gov)
  • These results suggest that interaction between EF-1α and axonemal MTs is weak and sensitive to treatment with 1% Triton X-100 and that EF-1α mediates between axonemal MTs and CaM in the presence of Ca 2+ . (elsevierpure.com)
  • Affected individuals with nystagmus may have CASK gene mutations that disrupt the interaction between the CASK protein and the protein produced from the FRMD7 gene, leading to problems with the development of the oculomotor neural network and resulting in abnormal eye movements. (medlineplus.gov)
  • Integrins are the cellular receptors for the proteins which constitute the extracellular matrix of all tissues. (upstate.edu)
  • The binding of integrin receptors to extraceullular proteins permits cell adhesion and migration during development, wound healing, and inflammation. (upstate.edu)
  • Here, we report the crystal structure of a CaM·Orai1-CMBD complex showing one CMBD bound to the C-terminal lobe of CaM, differing from other CaM-target protein complexes, in which both N- and C-terminal lobes of CaM (CaM-N and CaM-C) are involved in target binding. (rcsb.org)
  • Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. (creativebiomart.net)
  • Systemic lupus erythematosus is characterized by the production of plethora of autoantibodies which potentially drive immune-complex related inflammation in various tissues and organs [ 1 ]. (hindawi.com)
  • Early steps in this transition are complex, with at least 2 signaling pathways identified: 1 stops yeast growth and another, with a heat shock protein 70-type profile, initiates the assembly of proteins necessary for mycelial growth. (cdc.gov)
  • The co-chaperone Hep1 is required to prevent the aggregation of mitochondrial Hsp70 proteins. (cipsm.de)
  • Among these proteins , calmodulin, ATP synthase mitochondrial subunits alpha and delta, malate dehydrogenase and sperm equatorial segment protein 1 were shown to be linked with sperm fertility. (rsc.org)
  • Purification of the individual snRNPs U1, U2, U5 and U4/U6 from HeLa cells and characterization of their protein constituents. (cyphergenomics.com)
  • The heterochromatin-enriched HP1 proteins play a critical role in regulation of transcription. (cipsm.de)
  • The CASK protein is primarily found in nerve cells (neurons) in the brain, where it helps control the activity (expression) of other genes that are involved in brain development. (medlineplus.gov)
  • Mutations in the CASK gene affect the role of the CASK protein in brain development and function, resulting in the signs and symptoms of CASK -related intellectual disability. (medlineplus.gov)
  • The severe form of this disorder, MICPCH, is caused by mutations that eliminate CASK function, while mutations that impair the function of this protein cause the milder form, XL-ID with or without nystagmus. (medlineplus.gov)
  • However, their calcium-binding domain is restricted by calmodulin from metazoans. (mdpi.com)
  • In this study, we developed red GECI, called FRCaMP, using calmodulin (CaM) from Schizosaccharomyces pombe fungus as a calcium binding domain. (mdpi.com)
  • RBD, Ras-binding domain of Raf-1. (rupress.org)
  • To gain insight into the general applicability of this hypothesis, the involvement of calmodulin in neuroinflammation in Alzheimer's, amyotrophic lateral sclerosis, Huntington's disease, Parkinson's disease, frontotemporal dementia, and other dementias was explored. (biomedcentral.com)
  • The intimate role of CaMBPs in AD was recently reviewed reinforcing the role of CaM in binding to and regulating multiple key proteins adding further support for the Calmodulin Hypothesis of O'Day and Myre [ 41 , 47 ]. (biomedcentral.com)
  • A multifunctional calcium-calmodulin-dependent protein kinase subtype that occurs as an oligomeric protein comprised of twelve subunits. (curehunter.com)
  • Calmodulin Mutations in Human Disease. (nih.gov)
  • The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. (creativebiomart.net)
  • The laboratory utilizes techniques ranging from molecular biology to whole animal inflammation models, with an emphasis on cell biology and protein biochemistry. (upstate.edu)
  • abstract = "Calmodulin (CaM) is known to be a ciliary component. (elsevierpure.com)
  • Calcium-dependent signaling pathways and heat shock protein expression regulate dimorphism in Paracoccidioides brasiliensis and have broad implications for other pathogens. (cdc.gov)
  • Time-resolved DEER EPR and solid-state NMR afford kinetic and structural elucidation of substrate binding to Ca(2+)-ligated calmodulin. (nih.gov)
  • Note that the protein charges come from Amber 1994 /99 force field. (lu.se)
  • Description: A sandwich ELISA kit for detection of Calmodulin Like Protein 5 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (glideruniversity.org)
  • Description: A competitive ELISA for quantitative measurement of Rat Calmodulin like protein 5(CALML5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (glideruniversity.org)
  • Quantification of Glial Fibrillary Acidic Protein: Comparison of Slot-Immunobinding Assays with a Novel Sandwich ELISA. (epa.gov)
  • Western blot and ELISA were used to validate the selected protein (Validation phase: n = 60). (bvsalud.org)
  • What is the function of a protein with SRC homology-2 domains? (flashcardmachine.com)
  • We demonstrate for the first time that 4H-1,2,6-thiadiazin-4-one (TDZ) can function as a chemotype for the design of ATP-competitive kinase inhibitors. (diamond.ac.uk)
  • Yeast-form-specific genes identified in Histoplasma capsulatum function in calcium/calmodulin signaling pathways and sulphur metabolism. (cdc.gov)
  • Recombinant human PTPN2 protein (1-314) with a N-teminus His/GST-tag was expressed in insect cells. (creativebiomart.net)
  • Type 1 DM is caused by the autoimmune destruction of the pancreatic β-cells resulting in reduced insulin production ( 4 ). (spandidos-publications.com)
  • Stem Cells 13(Suppl 1):58-68. (cdc.gov)
  • The possibility of determining protein biomarkers for fertility is more useful and less expensive than ERCR for acquiring rapid estimation of fertility because it does not require the use of field insemination trials. (rsc.org)
  • This study aims to explore the changes in urinary exosomal proteins, which may provide novel biomarkers for diagnosing DKD. (bvsalud.org)
  • Calcium-dependent regulation of ER-α is critical for activating gene expression and is controlled by calmodulin (CaM). (rcsb.org)
  • Plastid-specific ribosomal proteins (PSRPs) have been proposed to play roles in the light-dependent regulation of chloroplast translation. (cipsm.de)
  • Results 1-1 of 1 (Search time: 0.002 seconds). (ub.edu)