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  • HOMEOSTASIS
  • Beyond their potential therapeutic value, these data provide insights into the intersection of calcium signaling with control of ion homeostasis and the ways in which the local CFTR microdomain organizes itself. (pnas.org)
  • Among the functional parameters that can be perturbed by interaction between nanoparticles (NPs) and living structures, calcium homeostasis is one of the key players and has been actively investigated. (mdpi.com)
  • receptor
  • The redistribution of CAPRI to the plasma membrane in transfected cells was stimulated by increases in intracellular Ca 2+ (either by treatment with a calcium ionophore or activation of the purinergic G protein-coupled receptor that activates phospholipase C leading to increased intracellular Ca 2+ owing to production of inositol trisphosphate). (sciencemag.org)
  • This chapter describes a method by which activating receptor-mediated calcium signaling can be measured in individual murine NK cells using a flow cytometer fitted with a UV laser. (nih.gov)
  • In cardiomyocytes and smooth muscle cells, cyclic AMP (cAMP) and subsequent calcium (Ca 2+ ) fluxes are the best characterized receptor-regulated signaling events. (ahajournals.org)
  • These observations led us to the discovery of the dopamine D1-D2 receptor heterooligomer, which is able to mobilize intracellular calcium ( 15 - 18 ). (pnas.org)
  • However, the signaling cascade and the physiological functions of the dopamine D1-D2 receptor heteromer in brain are unknown. (pnas.org)
  • For example, the occupancy of a cell surface receptor by a drug, hormone or a growth/differentiation factor often results, directly or indirectly, in a rise in the intracellular concentration of ionized calcium. (springer.com)
  • The inositol 1,4,5-trisphosphate- or ryanodine receptor-mediated [Ca 2+ ] c signal also induced a decrease in mitochondrial motility. (rupress.org)
  • Analysis of the subcellular distribution of Fyn kinase and the IP3 receptor reveal that these important signaling components are highly enriched in the oocyte cortex, a factor which may facilitate a faster propagation of the calcium transient in this compartment. (ehu.es)
  • Regulation
  • Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology explores the channels and pumps that transport calcium between different compartments and the regulation of calcium fluxes. (cshlpress.com)
  • Visualization of local store Ca 2+ signaling thus broadens our understanding of cardiac store Ca 2+ regulation and function and opens the possibility for local regulation of diverse store-dependent functions. (pnas.org)
  • Our results afforded insights into mechanisms underlying spark termination and refractoriness, intra-SR Ca 2+ communication, and local regulation of store Ca 2+ signaling. (pnas.org)
  • The regulation of intracellular Ca 2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols , and the developments in the field have also, naturally, continued. (springer.com)
  • cells
  • If you want to read about calcium signaling in different mammalian cells, oocytes, Zebrafishes, and even in plants, in one and the same book, then this book will not disappoint you. (waterstones.com)
  • Calcium signaling in lizard red blood cells. (biomedsearch.com)
  • The ion calcium is a ubiquitous second messenger, present in all eukaryotic cells. (biomedsearch.com)
  • To process this variety of information, the cells display a number of calcium pools, which are capable of mobilization for signaling purposes. (biomedsearch.com)
  • Here we review the calcium signaling on lizards red blood cells, an interesting model that has been receiving an increasing notice recently. (biomedsearch.com)
  • These cells possess a complex machinery to regulate calcium, and display calcium responses to extracellular agonists. (biomedsearch.com)
  • Many of these cellular players have been well characterized in terms of calcium signaling but astonishingly little is known about the consequences and functional role of such signaling at the very sites where these cells interact. (frontiersin.org)
  • This Research Topic seeks to bring together contributions that improve our understanding of how calcium signaling impacts on cellular interactions at the brain-vascular interface, how it alters the function of this interface and how the interface, on its turn, influences calcium signaling of its constituent cells. (frontiersin.org)
  • Calcium transient was found to elicit a plethora of biochemical and cellular events in these cells. (springer.com)
  • Cells use this external source of signal Ca2+ by activating various entry channels with widely different properties. (genome.jp)
  • However, calcium signaling is not so simple, there is a second, slower, more complex component that can be observed in cultured cells stimulated with high concentrations of K + . Confocal analysis of the calcium transients induced by high K + showed the rapid calcium transient in the cytosol and a second delayed calcium transient occurring in the nucleus. (sciencemag.org)
  • Treatment of cells with either a phospholipase C inhibitor or an IP3R inhibitor blocked the slow calcium signal induced by high K + . Furthermore, stimulated myotubes exhibit an activation of the mitogen-activated protein kinases, ERK1 and ERK2, and increased phosphorylation of the transcription factor CREB. (sciencemag.org)
  • In isolated nuclei from mammalian MC3T3.E1 cells treated to allow storage of calcium in the nuclear envelope, NAD+ or cADPr caused increases in the concentration of nucleoplasmic calcium. (sciencemag.org)
  • This review will discuss the machinery responsible for Ca 2+ signals in these cells, as well as experimental models used to investigate cholangiocyte Ca 2+ signaling. (mdpi.com)
  • Mean calcium flux data from NK cells stimulated with biotinylated anti-NK1.1 mAb (added at 60 sec) streptavidin (at 120 sec) are visualized by several smoothing methods. (nih.gov)
  • Plotting the ratio of mean indo violet/indo blue emission values at individual time points provides values corresponding to relative calcium concentration of cells within the population over the time course of the experiment. (nih.gov)
  • E. Calcium flux measurements of the subsets defined in D. above with the thin line representing immature NK cells and the thicker line representing mature NK cells. (nih.gov)
  • This experiment demonstrates that expression of PMCA2w/b dramatically shortens the duration of the Ca2+ signal compared to control cells that express mainly PMCA4b. (mayo.edu)
  • Here, we patch clamp single motile cilia of mammalian ependymal cells and examine their potential function as a calcium signaling compartment. (elifesciences.org)
  • We measured Ca V 1 voltage-gated calcium channels in ependymal cells, but these channels are not specifically enriched in motile cilia. (elifesciences.org)
  • Calcium is one of several intracellular signal transducers utilized by eukaryotic cells as part of their integrated responses to external stimuli. (springer.com)
  • It has been well established that Ca 2+ signals occurring inside cells affect activities within the nucleus. (biologists.org)
  • concentration
  • The influx of Ca2+ from the environment or release from internal stores causes a very rapid and dramatic increase in cytoplasmic calcium concentration, which has been widely exploited for signal transduction. (genome.jp)
  • In this exercise, you will study concentration and binding dynamics of calcium in a sim-plified model of a cell. (openwetware.org)
  • Calcium enters the cell in pulses called "transients," each of which corresponds to injection of a fixed concentration of calcium (delta[Ca2+]T) at an instant in time. (openwetware.org)
  • Resting motile cilia calcium concentration ([Ca 2+ ] ~170 nM) is only slightly elevated over cytoplasmic [Ca 2+ ] (~100 nM) at steady state. (elifesciences.org)
  • Although an increase in the concentration of nuclear Ca 2+ might not be the initiating signal for such events, it can be necessary for gene transcription to occur or to be sustained. (biologists.org)
  • Channels
  • These stimuli induce the formation of Ca 2+ signals within a cell, which are generated through the action of Ca 2+ release and uptake from and into internal cellular stores or the apoplast by the activity of Ca 2+ channels, pumps and exchangers. (els.net)
  • Calcium signals are generated through the coordinated action of calcium influx channels and calcium efflux transporters. (els.net)
  • This means researchers now have the tools to answer questions about how fast signals initiate, what terminates them, how quickly they terminate, how far they spread, how many channels it takes to kick off a signaling event, and many other questions that classical techniques simply couldn't answer. (brightsurf.com)
  • Nifedipine exerted maximum inhibition by blocking 30% to 35% of the peak AVP response, indicating the involvement of dihydropyridine-sensitive voltage-dependent calcium channels. (ahajournals.org)
  • These observations indicate that about one third of the AVP-induced constriction of renal resistance vessels is mediated by voltage-dependent L-type calcium channels responsive to the dihydropyridine nifedipine. (ahajournals.org)
  • We conclude that beating of ependymal motile cilia is not tightly regulated by voltage-gated calcium channels, unlike that of well-studied motile cilia and flagella in protists, such as Paramecia and Chlamydomonas . (elifesciences.org)
  • demonstrate that the activity of voltage-gated calcium channels does not control the beating rhythm of the motile cilia in the mouse brain or how quickly the fluid above the cell surface moves. (elifesciences.org)
  • Gold particles signaling BK channels were localized on NE (arrows) and mitochondria (M) (arrowhead) in wild-type mice. (nih.gov)
  • Redox-insensitive ORAI calcium channels enable monocytes to sustain calcium signaling while still producing bactericidal reactive oxygen species. (sciencemag.org)
  • regulate
  • These observations implicate epinephrine as a possible paracrine signal that could help regulate the hydration of the subretinal space. (jneurosci.org)
  • vivo
  • In a recently published article in The Journal of Neuroscience , Bonder and McCarthy (2014) extended these findings, demonstrating that Gq-GPCR-IP 3 R2-dependent calcium signaling in cortical astrocytes is neither necessary nor sufficient to generate functional hyperemia responses in the visual cortex of in vivo lightly sedated mice. (jneurosci.org)
  • modulate
  • In particular, we focus on the idea that the nucleus has an autonomous Ca 2+ signalling system that can generate its own Ca 2+ transients that modulate processes such as gene transcription. (biologists.org)
  • Nuclear
  • Blocking nuclear calcium signaling reversibly inhibits long-term memory formation in flies. (sciencemag.org)
  • These results suggest that the nBK channel functions as a molecular link between neuronal activity and nuclear Ca(2+) to convey signals from synapse to nucleus and is a new modulator, operating at the NE, of synaptic activity-dependent neuronal functions. (nih.gov)
  • Over the past 15 years or so, numerous studies have sought to characterise how nuclear calcium (Ca 2+ ) signals are generated and reversed, and to understand how events that occur in the nucleoplasm influence cellular Ca 2+ activity, and vice versa. (biologists.org)
  • In this Commentary, we describe mechanisms of nuclear Ca 2+ signalling and discuss what is known about the origin and physiological significance of nuclear Ca 2+ transients. (biologists.org)
  • This might also be the case for nuclear Ca 2+ signalling, which is essentially a complex form of local Ca 2+ signalling. (biologists.org)
  • As described below, the mechanisms of nuclear Ca 2+ signalling are extensive, and it is likely that there is considerable flexibility in how Ca 2+ signals are triggered in the nucleus and in the downstream targets that are affected. (biologists.org)
  • Collectively, these data suggest that nuclear Ca 2+ signals are important for regulating cell proliferation in some contexts. (biologists.org)
  • Methods
  • This new edition deals with methods for studying calcium from a variety of perspectives. (ebooks.com)
  • In this chapter, we describe the methods that allow reconstitution, detection, and quantification of the calcium signal in cell-free extracts of Xenopus oocytes and eggs. (springer.com)
  • Abstract
  • ABSTRACT Fertilization involves an initial, highly localized signal delivered by the sperm, which becomes amplified by a signal transduction cascade to impact the entire oocyte cytoplasm. (ehu.es)
  • biology
  • Including discussions of calcium signaling during development and normal physiology, as well as perturbed signaling in diseases such as diabetes, neurodegeneration, and atherosclerosis, the volume is an indispensable reference for biochemists, cell and developmental biologists, and physiologists seeking a comprehensive treatment of calcium biology. (cshlpress.com)