Ca2+/Calmodulin dependent protein kinase (CaMK) are mammalian calmodulin-dependent calcium-dependent protein kinases activated by elevation of Ca+2 and calmodulin concentration to phosphorylate Ser and Thr. (proteopedia.org)
For details see Calcium-dependent protein kinase . (proteopedia.org)
Rellos P, Pike AC, Niesen FH, Salah E, Lee WH, von Delft F, Knapp S. Structure of the CaMKIIdelta/calmodulin complex reveals the molecular mechanism of CaMKII kinase activation. (proteopedia.org)
A multifunctional calcium-calmodulin-dependent protein kinase subtype that occurs as an oligomeric protein comprised of twelve subunits. (curehunter.com)
It differs from other enzyme subtypes in that it lacks a phosphorylatable activation domain that can respond to CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE KINASE. (curehunter.com)
We found that Homer3, the predominant isoform in Purkinje cells, is phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) both in vitro and in vivo . (jneurosci.org)
In this study, we revealed that Homer3 was phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) in Purkinje cells, and the phosphorylation reduces the affinity for Homer target molecules, resulting in change of the solubility. (jneurosci.org)
It is shown that increasing of intracellular calcium concentration in rotavirus infected cells is associated with the activation of some members of protein kinases family such as calcium/calmodulin-dependent kinase II, which plays a crucial role in replication and pathogenesis of the virus. (ac.ir)
The aim of this study was to expression bovine rotavirus NSP4 gene in HEK293 cell and evaluation of its biological effect related to activation of calcium/calmodulin-dependent kinase II in cell culture. (ac.ir)
Western blot analysis was performed as a confirmation test for both expression of NSP4 protein and activation of calcium/calmodulin-dependent kinase II. (ac.ir)
Expression of NSP4 and activated form of calcium/calmodulin-dependent kinase II were demonstrated by western blotting. (ac.ir)
Autophagy hijacked through viroporin-activated calcium/calmodulin-dependent kinase kinase-β signaling is required for rotavirus replication. (ac.ir)
Autophosphorylation at Thr286 of the α calcium-calmodulin kinase II in LTP and learning. (ac.ir)
12. Braun AP, Schulman H. The multifunctional calcium/calmodulin-dependent protein kinase: from form to function. (ac.ir)
13. Meyer T, Hanson PI, Stryer L, Schulman H. Calmodulin trapping by calcium-calmodulin-dependent protein kinase. (ac.ir)
After the influx of calcium ions and the binding to calmodulin, pp60 SRC (a protein kinase)causes a conformational change in MYLK, activating it and resulting in an increase in phosphorylation of myosin light chain at serine residue 19. (wikipedia.org)
The CASK gene provides instructions for making a protein called calcium/calmodulin-dependent serine protein kinase (CASK). (medlineplus.gov)
Calcium mineral/calmodulin-dependent proteins kinase II (CaMKII) can be an autophosphorylating kinase (14,15) that constitutes up to 2% of total proteins in certain parts of the brain and it is extremely enriched at synapses (16). (healthyguide.info)
In this study, we showed that most of the kinase activity phosphorylating FRQ in vitro was calcium/ calmodulin-dependent, and the endogenous FRQ in the Neurospora extracts was phosphorylated by a Ca/ CaM-dependent kinase-like activity. (elsevierpure.com)
Methods: To unravel the mechanism of action of ketamine, we treated wild-type C57BL/6 mice with calcium/calmodulin-dependent protein kinase II (CaMKII) specific inhibitor tatCN21 peptide. (haifa.ac.il)
Calcium/calmodulin-dependent protein kinase kinase (CaMKK) is a major kinase that is activated by elevated intracellular calcium. (biomedcentral.com)
Calcium/calmodulin-dependent protein kinase kinase (CaMKK) is a major kinase that is activated by elevated intracellular calcium and is highly abundant in the brain [ 8 ]. (biomedcentral.com)
In cells lacking calmodulin kinase 2 (Cmk2p), a secondary rise in cytosolic calcium concentration is seen after extracellular calcium increases and the cytosolic calcium is subsequently sequestered. (azregents.edu)
In liver I/R, members of the calcium/calmodulin-dependent protein kinase (CaMK) family are known to be activated, but their individual roles are largely unknown. (usuhs.edu)
Immunohistochemical and biochemical investigations of Ca2+/calmodulin-dependent protein kinase II(CaM kinase II) and protein phosphatase (calcineurin) after transient forebrain ischemia demonstrated that the activity of CaM kinase II was decreased in the CA1 region of the hippocampus early (6-12 hours) after ischemia. (nih.gov)
Ca2+/Calmodulin-dependent protein kinase 2 (CAMK2) family proteins are involved in regulation of cellular processes in a variety of tissues including brain, heart, liver and kidney. (bvsalud.org)
Proteins10
Calmodulin is an important multifunctional molecule that regulates the activities of a large number of proteins in the cell. (biomedcentral.com)
Calcium binding induces conformational transitions in calmodulin that make it specifically active to particular target proteins. (biomedcentral.com)
Increased phosphorylation of brain, but not spinal cord, axolemmal proteins in the presence of added Ca2+ and calmodulin was observed in TOCP treated hens. (cdc.gov)
140 mouse brain proteins identified by Ca2+-calmodulin affinity chromatography and tandem mass spectrometry. (lu.se)
We describe the proteomic characterization of mouse brain Ca2+-calmodulin-binding proteins that were purified using calmodulin affinity chromatography. (lu.se)
A total of 140 putative Ca2+-calmodulin-binding proteins were identified of which 87 proteins contained calmodulin-binding motifs. (lu.se)
Among the 87 proteins that contained calmodulin-binding motifs, 48 proteins have not previously been shown to interact with calmodulin and 39 proteins were known calmodulin-binding proteins. (lu.se)
One potential candidate is calbindin D 9k, a monomeric calcium-binding protein of the S100 family of the calmodulin-related proteins, involved in Ca 2+ buffering and in trans-cellular transport of Ca 2+ ions. (lu.se)
CaMKII1
CaMKII is certainly turned on by calcium-saturated calmodulin (CaM) (17), as soon as activated, it could autophosphorylate at T286 (18). (healthyguide.info)
Dependent10
The results of our analysis reduce the gap between current understanding of intracellular calmodulin function at the structural level and physiological calcium-dependent calmodulin target activation experiments. (biomedcentral.com)
The rotavirus nonstructural protein 4 (NSP4) is responsible for the increase in cytoplasmic calcium concentration through a phospholipase C-dependent and phospholipase C-independent pathways in infected cells. (ac.ir)
Voltage-dependent calcium channels (Ca(V)) open in response to changes in membrane potential, but their activity is modulated by Ca(2+) binding to calmodulin (CaM). (rcsb.org)
Calcium-dependent regulation of ER-α is critical for activating gene expression and is controlled by calmodulin (CaM). (rcsb.org)
The interaction is calcium-dependent and is mediated by a calmodulin-binding domain between Leu-296 and Leu-314 close to the C-proximal end of the pea Tic32. (ox.ac.uk)
These results support the hypothesis that NMDA modulation of voltage-gated sodium channels in horizontal cells requires CICR and activation of a calmodulin-dependent signaling pathway. (nih.gov)
We hypothesized that an imbalance of Ca2+/calmodulin dependent protein phosphorylation-dephosphorylation may be involved in delayed neuronal death after ischemia. (nih.gov)
In cultured keratinocytes, CWO stimulated calcium signaling, resulting in calcineurin-dependent activation of nuclear factor of activated T cells (NFAT). (biorxiv.org)
Calcium-dependent signaling pathways and heat shock protein expression regulate dimorphism in Paracoccidioides brasiliensis and have broad implications for other pathogens. (cdc.gov)
Using affinity-chromatography on calmodulin-agarose, we could identify the inner envelope translocon component Tic32 as the predominant calmodulin-binding protein of this membrane. (ox.ac.uk)
A Ca2+-ATPase was purified from plasma membranes (PM) isolated from Arabidopsis cultured cells by calmodulin (CaM)-affinity chromatography. (unimi.it)
Ions2
these Calcium ions, in turn stimulate a MYLK, forcing a contraction. (wikipedia.org)
Utilizing double deletions, we demonstrate that Cmk2p is modulating the activity of Rch1p, a known inhibitor of Channel X which is a yeast plasma membrane channel through which calcium ions are transported after an extracellular calcium pulse. (azregents.edu)
Phosphorylation1
Reducing intracellular calcium concentration inactivates MLCK but does not stop smooth muscle contraction since the myosin light chain has been physically modified through phosphorylation(and not via ATPase activity). (wikipedia.org)
Metabolism1
Yeast-form-specific genes identified in Histoplasma capsulatum function in calcium/calmodulin signaling pathways and sulphur metabolism. (cdc.gov)
Regulation5
Calcium (Ca 2+ ) signaling plays an important role in the regulation of many cellular functions. (frontiersin.org)
Calcium regulation of chloroplast protein translocation is mediated by calmodulin binding to Tic32. (ox.ac.uk)
Regulatory circuits include redox-control as well as calcium-regulation, with calmodulin being the likely mediator of the latter. (ox.ac.uk)
These results suggest that redox modulation and calcium regulation of chloroplast protein import convene at the Tic translocon and that both could be mediated by Tic32. (ox.ac.uk)
Binds1
Calmodulin is an essential Ca2+-binding protein that binds to a variety of targets that carry out critical signaling functions. (lu.se)
Mechanism1
Calmodulin (CaM)-mediated Ca2+-induced inactivation is an ion channel regulatory mechanism that protects cells against the toxic effects of Ca2+ overload. (columbia.edu)
Cells9
Expression of type I adenylyl cyclase activity in animal cells has established that this enzyme is stimulated by calmodulin and Ca2+. (nih.gov)
In this study, type III adenylyl cyclase was expressed in human kidney 293 cells to determine if the enzyme is stimulated by Ca2+ and calmodulin. (nih.gov)
5. Michelangeli F, Ruiz M-C, Del Castillo JR, Ludert JE, Liprandi F. Effect of rotavirus infection on intracellular calciumhomeostasis in cultured cells. (ac.ir)
The rotavirus enterotoxin NSP4 mobilizes intracellular calcium in human intestinal cells by stimulating phospholipase C-mediated inositol 1, 4, 5-trisphosphate production. (ac.ir)
NMDA channels were activated in voltage-clamped cells incubated in low-calcium saline or dialyzed with the calcium chelator BAPTA to determine that calcium influx through NMDA channels is required for sodium channel modulation. (nih.gov)
To determine whether calcium influx through NMDA channels triggers calcium-induced calcium release (CICR), cells were loaded with the calcium-sensitive dye calcium green 2 and changes in relative fluorescence were measured in response to NMDA. (nih.gov)
Blocking NMDA receptors with AP-7, blocking CICR with ruthenium red, depleting stores with caffeine, or dialyzing cells with calmodulin antagonists W-5 or peptide 290-309 all prevented sodium channel modulation. (nih.gov)
The concentration drops rapidly in these cells as the calcium is sequestered away in the endoplasmic reticulum, Golgi, and vacuoles leaving resting cytosolic levels higher than their original state, followed by changes in gene expression. (azregents.edu)
Calmodulin is a protein found in all living organisms, or at least those whose cells have a nucleus. (pasteur.fr)
Structural1
In this study, we adopt a structural systems biology approach and develop a mathematical model to investigate various types of cooperative calcium-calmodulin interactions. (biomedcentral.com)
Influx1
Once there is an influx of calcium cations (Ca2+) into the muscle, either from the sarcoplasmic reticulum or from the extracellular space, contraction of smooth muscle fibres may begin. (wikipedia.org)
Domain5
However, their calcium-binding domain is restricted by calmodulin from metazoans. (mdpi.com)
In this study, we developed red GECI, called FRCaMP, using calmodulin (CaM) from Schizosaccharomyces pombe fungus as a calcium binding domain. (mdpi.com)
Calmodulin - A new domain in osteoporosis treatment. (lcngrain.com)
Binding of calcium ion to this domain increases the affinity of MYLK binding to myosin light chain. (wikipedia.org)
At-ACA8 encodes a plasma membrane-localized calcium-ATPAse of arabidopsis with a calmodulin-binding domain at the N terminus / M.C. Bonza, P. Morandini, L. Luoni, M. Geisler, M.G. Palmgren, M.I. De Michelis. (unimi.it)
Physiological2
Calcium (Ca2+) plays a major role in numerous physiological processes. (columbia.edu)
We compare the predictions of our analysis with physiological dose-response curves taken from the literature, in order to provide a quantitative comparison of the effects of different mechanisms of cooperativity on calcium-calmodulin interactions. (biomedcentral.com)
Concentration1
After a single extracellular 100 mM calcium pulse (final concentration), wild type S. cerevisiae exhibits a sharp peak in cytosolic calcium. (azregents.edu)
Genetically1
Red fluorescent genetically encoded calcium indicators (GECIs) have expanded the available pallet of colors used for the visualization of neuronal calcium activity in vivo. (mdpi.com)
Induces1
8. Berkova Z, Crawford S, Trugnan G, Yoshimori T, Morris A, Estes M. Rotavirus NSP4 induces a novel vesicular compartment regulated by calcium and associated with viroplasms. (ac.ir)
Enzyme2
The type III enzyme was not stimulated by Ca2+ and calmodulin in the absence of other effectors. (nih.gov)
It was, however, stimulated by Ca2+ through calmodulin when the enzyme was concomitantly activated by either GppNHp or forskolin. (nih.gov)
Calbindin D 9k1
We investigate the solution structure of calbindin D 9k using SAXS and quantify the protein-protein interactions through second virial coefficients as a function of the calcium binding state (holo and apo) and the screening conditions, both experimentally and via Monte Carlo computer simulations. (lu.se)
Activation of mAChRs is relatively slow (milliseconds to seconds) and, depending on the subtypes present (M1-M5), they directly alter cellular homeostasis of phospholipase C, inositol trisphosphate, cAMP, and free calcium. (genome.jp)
The small and highly expressed Ca 2+ -binding protein calmodulin (CaM) acts as a primary effector of calcium function ( Soderling and Stull, 2001 ). (frontiersin.org)
Channel1
We used cryo-electron microscopy to capture the epithelial calcium channel TRPV6 (transient receptor potential vanilloid subfamily member 6) inactivated by CaM. (columbia.edu)
Activity1
At the same time, dehydrogenase activity of Tic32 is affected by calmodulin. (ox.ac.uk)
Type III1
The sensitivity of the type III adenylyl cyclase to Ca2+ and calmodulin has not been reported. (nih.gov)
Studies1
According to the most current studies, the most important CBP (Calcium Binding Protein) in the human body is Calmodulin. (lcngrain.com)