• SMOC2 is a known person in the BM-40/osteonectin category of calcium-binding secreted matricellular proteins. (neighborhoodhousecharterschool.org)
  • SMOC2 (SPARC-related modular calcium-binding protein 2) can be a secreted calcium-binding protein through the BM-40/SPARC/osteonectin category of secreted matricellular proteins. (neighborhoodhousecharterschool.org)
  • Binding of CAPN3 to titin, a huge myofibrillar proteins that acts as a scaffold for sarcomeric. (exposed-skin-care.net)
  • It has also been discovered that magnesium binds to over 3,751 binding sites on human proteins. (my-sunshine.ca)
  • The toxic effects of lead result from its interference with the physiologic actions of calcium, zinc, and iron, through the inhibition of certain enzymes, and through binding to ion channels and regulatory proteins. (cdc.gov)
  • Ionized calcium binds to negatively charged sites on protein molecules, competing with hydrogen ions for the same binding sites on albumin and other calcium-binding proteins. (medscape.com)
  • Membrane-bound and secreted proteins are made on ribosomes found in the rough endoplasmic reticulum . (wikipedia.org)
  • As you can see from the makeup of the actual bone structure, in order for that remodeling to happen, you need to intake minerals, proteins, and fats to make up the structure of the bones. (weightandwellness.com)
  • The 1,25-dihydroxyvitamin D3-VDR complex binds to these genes, resulting in the recruitment of hnRNP C1/C2 and other proteins that can compete with the repressors and initiate transcription. (liquorrs.com)
  • The spermidine transporter of consists of five proteins, i.e., the periplasmic substrate-binding protein SpuD and SpuE, the ATPase SpuF, as well as the internal membrane permeases SpuG and SpuH that constitute the cross-membrane route18. (phytid.org)
  • Our newer research demonstrated that SpuD is certainly a putrescine-preferential binding proteins, andSpuE is certainly a spermidine-specific binding proteins19. (phytid.org)
  • After an intracellular calcium mineral influx CaM and CaNB P529 bind with their focus on sites on CaNA triggering a conformational transformation resulting in the displacement from the autoinhibitory domains in the enzymatic site (Wang et al. (conferencedequebec.org)
  • A significant pathway triggered from the TCR may be the creation of intracellular calcium mineral as CORM-3 well as the activation from the transcription element nuclear element of triggered T-cells (NFAT). (researchassistantresume.com)
  • Around the newly formed matrix de-novo enamel-crystals are formed from calcium phosphate present in saliva. (wikipedia.org)
  • Medical device for caries treatment and enamel regeneration: CURODONT REPAIR (EU) REGENAMEL (CH) Cosmetic products for acid protection and dentin desensitization: CURODONT PROTECT (EU) EMOFLUOR PROTECT GEL PROFESSIONAL (CH) CURODONT D'SENZ (EU & CH) EMOFLUOR DESENS GEL PROFESSIONAL (CH) Candida Protect Professional (CH) Medicine portal Amorphous calcium phosphate (Recaldent) Remineralisation of teeth Oligopeptide Biomimetic materials Fluoride Brunton, P.A. (wikipedia.org)
  • interaction with PTH (parathyroid hormone) to absorb calcium from the intestine and reabsorption of calcium into the kidneys, as well as to regulate the excretion of inorganic phosphate. (wakeup-world.com)
  • Rocaltrol is indicated for the correction of the abnormalities of calcium and phosphate metabolism in patients with renal osteodystrophy. (medicines.org.uk)
  • By binding to calcium salts, alendronate blocks the transformation of calcium phosphate into hydroxyapatite and inhibits the formation, aggregation, and dissolution of hydroxyapatite crystals in bone. (medscape.com)
  • When the food has a lot of calcium phosphate and zinc deficiency can be difficult. (dkart.in)
  • To achieve this, calcium and phosphate-based amendments will be injected into the ground allowing for the opportunity to bind with the dissolved uranium to produce more stable uranium-based minerals. (nmisite.org)
  • Together with vitamin D and calcitonin, PTH brings about the mobilization of calcium and phosphate from the skeletal system and increases the uptake of calcium in the intestine and the excretion of phosphate via the kidneys. (cdc.gov)
  • The main effects of parathyroid hormone are to increase the concentration of plasma calcium by increasing the release of calcium and phosphate from bone matrix, increasing calcium reabsorption by the kidney, and increasing renal production of 1,25-dihydroxyvitamin D-3 (calcitriol), which increases intestinal absorption of calcium. (medscape.com)
  • It builds a 3-D bio-matrix with binding sites for calcium ions serving as nucleation point for hydroxyapatite (tooth mineral) formation. (wikipedia.org)
  • FOSCAVIR has the potential to chelate divalent metal ions, such as calcium and magnesium, to form stable coordination compounds. (nih.gov)
  • Calcium ions in the blood are so vital that the body cannot permit its level to fluctuate. (vieuxquebec.com)
  • The sarcoplasmic reticulum on the cardiac muscular cell phone stores and produces calcium supplement ions for contraction. (casasantalucia.it)
  • Contraction will involve a healthy connections among calcium supplement ions, cell method of travel systems of calcium supplements, contractile protein as well as significant power phosphates. (casasantalucia.it)
  • An inward influx on the extracellular calcium ions via the ion routes (calcium mineral) in the T tubules offers depolarization of your cardiac muscles tissue for an extended time. (casasantalucia.it)
  • The operation of contraction is not going to come to pass for a bottom reaction to connections involving calcium mineral ions and contractile protein but ATP represents a serious purpose in presenting power which critical for the procedure of contraction and rest. (casasantalucia.it)
  • For example, chondrocalcinosis can be a skeletal disorder where calcium mineral pyrophosphate crystals are transferred in GPDA the tendons and bones, triggering painful and acute inflammation [2]. (neighborhoodhousecharterschool.org)
  • Calcium metabolism modifiers such as bisphosphonates are stable analogues of inorganic pyrophosphate. (medscape.com)
  • This study was performed to test the hypohesis that EHDP would interfere with the process of calcium uptake and deposition in evolving myocardial infarction and thereby influence other parameters, including technetium-99m pyrophosphate (Tc-99m PYP) uptake and scintigraphic visualization of the infarcts. (elsevierpure.com)
  • FOSCAVIR exerts its antiviral activity by a selective inhibition at the pyrophosphate binding site on virus-specific DNA polymerases at concentrations that do not affect cellular DNA polymerases. (nih.gov)
  • Pyrophosphate helps control deposition of calcium (calcification) and other minerals (mineralization) in the body. (medlineplus.gov)
  • As a result, little pyrophosphate is produced and calcium accumulates in the blood vessels and other tissues affected by GACI. (medlineplus.gov)
  • Mineralization in MC3T3-E1 cells overexpressing mutant SMOC2 missing the extracellular calcium-binding site was significantly improved in comparison to cells overexpressing complete size SMOC2. (neighborhoodhousecharterschool.org)
  • When SMOC2 overexpressing cells had been cultured in the current presence of extracellular calcium mineral supplementation, SMOC2s inhibitory influence on calcification was rescued. (neighborhoodhousecharterschool.org)
  • Calcification from the bone tissue extracellular matrix provides body and bone tissue framework, helps to shield the internal organs and it is a storage space site that calcium mineral could be mobilized when needed. (neighborhoodhousecharterschool.org)
  • BM-40/SPARC/osteonectin family all consist of an extracellular calcium-binding (EC) site, a follistatin-like (FS) site and an acidic N-terminal site. (neighborhoodhousecharterschool.org)
  • The motions opportunity initiates the calcium supplement routes inside of the T-tubules triggering increase in cytosolic calcium mineral heights by influx of calcium supplement belonging to the extracellular area and the launch of calcium of the sarcoplasmic reticulum. (fucp.uk)
  • Host get in touch with and low calcium mineral are two powerful inducing indicators, and metabolic tension, DNA harm, high concentrations of extracellular Cu2+, and low osmolarity are also implicated as repressive indicators13. (phytid.org)
  • Through their secretion of parathyroid hormone (PTH), the parathyroid glands are primarily responsible for maintaining extracellular calcium concentrations. (medscape.com)
  • This approach loses sight of the fact that many factors will mediate how 25-hydroxyvitamin D is converted to the active secosteroid hormone calcitriol (1,25-dihydroxyvitamin D) and the most potent metabolite of vitamin D. In addition, many pathogenic influences will alter the course of intracellular transport of calcitriol and nuclear binding to the vitamin D receptor (VDR). (wakeup-world.com)
  • These functions are activated when calcitriol binds to the site of the VDR nuclear receptor. (wakeup-world.com)
  • Ethane-l-hydroxy-1,1-diphosphonate (EHDP) inhibits bone mineral growth. (elsevierpure.com)
  • Random supplementation with high doses of vitamin D without knowing and understanding the person's entire health profile or the condition of other minerals and vitamins involved in biochemical balance can be even dangerous. (wakeup-world.com)
  • As a source of calcium, MCHC contributes to the production and maintenance of bone structure, a function supported by vitamins D3 and K2. (aliveandwell.health)
  • With the use of synthetic fertilizers, depletion of the soil and modern manufacturing, supplementation with minerals (and vitamins ) supplies needed nutrients that our bodies can no longer obtain from the food supply. (autismsupplementscenter.com)
  • The World Health Organization indicates over 2 billion people suffer from a dietary deficiency of vitamins and minerals. (iherb.com)
  • And in the US, data from the National Health and Nutrition Examination Survey (NHANES) and the United States Department of Agriculture's Food Consumption and Nutrient Intakes for Americans show nutrient deficiencies exist in a substantial portion of the US population, perhaps as high as 80% for some vitamins and minerals. (iherb.com)
  • Vitamins and minerals are essential nutrients, meaning the body cannot function correctly without them. (iherb.com)
  • One of the main functions of vitamins and minerals is that they are found in the active part of the enzymes, and coenzymes work together to build or break down molecules. (iherb.com)
  • Nutrient density reflects the amount of protein, vitamins, and minerals per 100 kcal of food. (medscape.com)
  • Why Vitamins & Minerals? (fitnessrxwomen.com)
  • Vitamins and minerals are micronutrients required by the body to catalyze and facilitate the biochemical reactions that convert the food we eat into useable nutrients that enable muscle growth and an optimal metabolism. (fitnessrxwomen.com)
  • Although food provides us with vitamins and minerals, it can take quite a bit of eating to get an adequate amount that is needed to maximize your efforts in the gym, rev up the metabolism and support the recovery processes. (fitnessrxwomen.com)
  • virtually every energy production process is dependent on the right proportion of vitamins and minerals. (fitnessrxwomen.com)
  • If you are serious about your fitness, you need to make sure you are getting enough of the right vitamins and minerals, especially if you are following a strict diet that might be deficient in the key nutrients needed to keep you functioning optimally. (fitnessrxwomen.com)
  • The best defense is to ensure you are incorporating a few key vitamins and minerals into your nutrition plan. (fitnessrxwomen.com)
  • These vitamins and minerals will make sure you are getting what you need to keep going, ensure proper recovery, avoid injury and keep your energy levels up! (fitnessrxwomen.com)
  • Nevertheless, ectopic or extreme calcification plays a part in diseases such as for example chondrocalcinosis, to calcium mineral deposits in your skin or even to vascular calcification. (neighborhoodhousecharterschool.org)
  • These findings suggests that calcification inhibitors, and possibly other calcium-blocking agents, may alter the sensitivity of Tc-99m PYP myocardial scintigraphy, but the specific effects of each calcium antagonist must be evaluated. (elsevierpure.com)
  • Polymorphisms at the ligand binding site of the vitamin D receptor gene and osteomalacia. (cdc.gov)
  • Little is known about the effect of the recently developed calcimimetic evocalcet (Evo) on parathyroid calcium-sensing receptor (CaSR) and vitamin D receptor (VDR) expression. (bvsalud.org)
  • Calcium mineral into the cytoplasm then binds to cardiac troponin-C, which moves the troponin-tropomyosin tricky away from the actin binding blog. (fucp.uk)
  • This will cause the cardiac troponin intricate to go back to its initial suppressing position over the energetic web site of actin which eventually stops contraction resulting in rest. (casasantalucia.it)
  • Once muscle cells receive a signal from the corresponding nerve, calcium floods into the stimulated muscle cells and binds with a protein called troponin. (fitnessrxwomen.com)
  • It is involved in the functioning of the sweat glands, activating local hormones, creating a protein that binds vitamin A, in control of inflammation and tissue regeneration. (dkart.in)
  • Calcium is essential for bone mineralization, neuromuscular function, and secretion of hormones and enzymes. (medscape.com)
  • The interaction is controlled by the reciprocal and temporal pattern of on- and off occupancy of the VDRE by the two components, and is modulated through chromosomal sites and binding preferences for certain hormones. (liquorrs.com)
  • The regulation of mineral homeostasis involves the three mineralotropic hormones PTH, FGF23 and 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). (bvsalud.org)
  • Bisphosphonates have a high affinity for hydroxyapatite crystals, and by binding at sites of active bone resorption, these agents can inhibit osteoclastic resorption. (medscape.com)
  • These crystals of calcium oxalate and calcium carbonates deter herbivores and also store minerals for the cell. (sciencenotes.org)
  • These results offer proof indicating that cell routine change from G0/G1 to G2/M and S stages is certainly accountable, at least partly, for the elevated adhesion of COM crystals on restoring renal tubular cells on the wounded site. (biopaqc.com)
  • Introduction Advancement of kidney rock disease requires extreme binding of causative crystalline contaminants to renal tubular epithelium, resulting in invasion and retention of the crystals into renal interstitium1C3. (biopaqc.com)
  • Effects of polyelectrolytic peptides on the quality of mineral crystals grown in vitro. (heightquest.com)
  • Morphology of resulting mineral crystals was investigated. (heightquest.com)
  • Most enzymes and coenzymes are composed of a protein and a co-factor, typically an essential mineral and/or a vitamin. (iherb.com)
  • Magnesium also directly competes with calcium for some of its binding sites, allowing greater binding of calcium to enzymes in hypomagnesaemia. (dlequine.co.nz)
  • The effectiveness of treatment depends in part on an adequate daily intake of calcium, which should be augmented by dietary changes or supplements if necessary. (medicines.org.uk)
  • An abrupt increase in calcium intake as a result of changes in diet (e.g. increased consumption of dairy products) or uncontrolled intake of calcium preparations may trigger hypercalcaemia. (medicines.org.uk)
  • The level of an individual vitamin and mineral intake can range from severe deficiency to toxicity. (iherb.com)
  • Use of calcitonin-salmon injection is recommended in conjunction with adequate calcium and vitamin D intake to prevent the progressive loss of bone mass. (medscape.com)
  • the recommended intake ranges from 240 mg per day for infants.It is not recommended to exceed 1,500 mg daily calcium intake. (vieuxquebec.com)
  • The recommended daily intake of calcium for a healthy adult is 1,000 milligrams. (fitnessrxwomen.com)
  • Magnesium is essential for calcium absorption, as well for nearly every biological function, including glucose metabolism. (boydsalternativehealth.com)
  • Journal of bone and mineral metabolism 2004 22 (1): 39-43. (cdc.gov)
  • Bone mineral density and calcium metabolism in adolescents with beta-thalassemia major. (cdc.gov)
  • Journal of bone and mineral metabolism 2017 12 36 (6): 723-733. (cdc.gov)
  • Pregnancy is characterized by physiologic changes in mineral metabolism, to allow calcium accretion in the fetal skeleton [ 1-3] . (who.int)
  • All therapies should be given with adequate calcium and vitamin D intakes. (medscape.com)
  • An ionized calcium value within the reference range implies adequate calcium homeostasis, as this is a direct measure of calcium in its active form. (medscape.com)
  • This product is ideally suited to young and old alike, for those that need an extra source of calcium and may have difficulty swallowing enough capsules to obtain adequate calcium, and for those who have sensitive stomachs as they may experience constipation or upset stomach with calcium capsule compounds. (vieuxquebec.com)
  • If adequate calcium is not available in the muscle, full hard contractions during weight training cannot be sustained. (fitnessrxwomen.com)
  • As a result of contraction the intracellular calcium is going to be cleaned up and removed in the sarcoplasmic reticulum, losing cytosolic calcium supplement attention. (fucp.uk)
  • From the coronary heart lean muscle, excitation contraction coupling depends upon the calcium mineral-stimulated calcium launch sensation where calcium supplements causes launch of more calcium supplements coming from the lean muscle sarcoplasmic reticulum. (casasantalucia.it)
  • The sarcoplasmic reticulum removes intracellular calcium supplements through the cellular shedding its intracellular attentiveness. (casasantalucia.it)
  • Choline-stabilized orthosilicic acid supplementation as an adjunct to calcium/vitamin D3 stimulates markers of bone formation in osteopenic females: a randomized, placebo-controlled trial. (healthmasters.com)
  • New Beginnings Nutritionals Chelate-Mate™ is a premier mineral replacement formula ideal for chelation support or for every day mineral supplementation. (autismsupplementscenter.com)
  • Magnesium is an essential mineral to human health and is involved in nearly every bodily function, from the beating of the heart to the creation of bones and the regulation of blood sugar. (my-sunshine.ca)
  • Zinc plays an important role in the absorption of calcium in the bones and affects the action of growth hormone. (dkart.in)
  • Since we've all heard that calcium is important for healthy bones, it is often the first thing people think of when wanting to create strong bones. (weightandwellness.com)
  • Calcium is vital for the formation of your strong bones, teeth, and gums. (vieuxquebec.com)
  • Calcium is also needed for the integrity of bones, which help support increased muscle tissue and provide an anchor during muscular contraction. (fitnessrxwomen.com)
  • Calcium is essential for muscular contraction and is needed to keep bones strong to support increases in muscle tissue, providing a solid anchor for muscle contraction. (fitnessrxwomen.com)
  • The resulting higher molecular structure has a high affinity to tooth mineral. (wikipedia.org)
  • The high affinity to tooth mineral is based on matching distances of Ca-ion binding sites on P11-4 and Ca spacing in the crystal lattice of hydroxyapatite. (wikipedia.org)
  • Renal tubular cell injury can boost calcium oxalate monohydrate (COM) crystal adhesion on the wounded site and therefore may raise the natural stone risk. (biopaqc.com)
  • The most frequent causative crystal type within 70C80% of rock formers (sufferers with kidney rock(s)) is calcium mineral oxalate monohydrate (COM)4. (biopaqc.com)
  • Association between serum calcium level and in-hospital mortality in patients with acute myocardial infarction: a retrospective cohort study. (vieuxquebec.com)
  • It is crucial in the younger years to lay a good foundation by avoiding sugar and intaking high-quality nutrients and minerals. (weightandwellness.com)
  • If we only focus on calcium, we are only adding the nutrients needed for the outside structure of the bone and we need that meshy inside layer to be both dense and supple to prevent fractures. (weightandwellness.com)
  • We focus here mostly on the mineral nutrients that are critical for healthy plants, but some trace elements are also important for animal and human health, including zinc, iron, iodine, calcium, magnesium, selenium and fluorine, which need to be supplied through the food chain (soil-plant-animal/human) or added as nutritional supplements. (sare.org)
  • The contraction and peace is finished all through the calcium-stimulated calcium introduce method therefore it follows actin myosin sliding filament product (Melts 2013). (fucp.uk)
  • This causes another protein, tropomyosin, to go away from the binding site of myosin, which is a thicker protein that bends and elongates to create muscle contraction. (fitnessrxwomen.com)
  • These elements interfere with vital minerals such as calcium, magnesium, zinc and others, replacing them in enzyme binding sites. (arltma.com)
  • Magnesium, also known as the "antistress" mineral, is a vital catalyst in enzyme activity. (boydsalternativehealth.com)
  • If an enzyme lacks the essential mineral or vitamin, it becomes inactive. (iherb.com)
  • If an enzyme lacks the essential mineral or vitamin, it cannot function properly. (iherb.com)
  • The enzyme can perform its vital function by providing the necessary mineral/vitamin through diet or a nutritional formula. (iherb.com)
  • Magnesium is a major mineral involved in over 300 enzyme activities in the body. (dlequine.co.nz)
  • Vanadium is a critical trace mineral that enhances insulin signaling and blood sugar balance by inhibiting the tyrosine phosphatase enzyme. (naturalnews.com)
  • We speculate that the relationship between Cu and fatty acids could be related to a requirement for Cu by an enzyme required for C10-C14 fatty acid biosynthesis (e.g. decanoyl deacylase) in mammary tissue, or to some unique Cu binding properties of the intermediate chain length fatty acids. (cdc.gov)
  • 2004). Effects of aging and postmenopausal hypoestrogenism on skin elasticity and bone mineral density in Japanese women. (healthmasters.com)
  • A variant of the gene, the rs2228570 (FokI polymorphism) is associated with decreased bone mineral density in white prepubescent girls. (liquorrs.com)
  • 25-hydroxyvitamin D [25(OH)D] level, and to positively impact infant bone mineral content (BMC). (who.int)
  • Calcium is a mineral not only essential for bone health, but it's also required for muscular contraction. (fitnessrxwomen.com)
  • Magnesium permits nerve chemical messengers to stimulate the muscle cell so that calcium can enter muscle cells, resulting in muscular contraction. (fitnessrxwomen.com)
  • Absorption of ingested lead can be as much as five times greater in children than adults and even greater when intakes of dietary minerals are deficient. (cdc.gov)
  • What is much more common in reality is competitive mineral interactions, which result in magnesium intakes or availability below optimal levels. (dlequine.co.nz)
  • It binds directly as matrix to the tooth mineral and forms a stable layer on the teeth. (wikipedia.org)
  • Total calcium level decreases during pregnancy, due to hemodilution, while the ionized calcium level remains stable [2 ]. (who.int)
  • Furthermore, the calcium focus needed for activation is normally in the nanomolar range (weighed against micro and millimolar concentrations for calpain 1 and 2, correspondingly) (19). (exposed-skin-care.net)
  • Secretion of PTH is inhibited by high calcium concentrations and is promoted by low calcium concentrations. (cdc.gov)
  • So there is a very strong connection between calcium and vitamin K, and if we are deficient in vitamin K, calcium can accumulate and deposit in the soft tissues of the body. (wakeup-world.com)
  • Without MRP6 function, calcium and other minerals accumulate in elastic fibers of the skin, eyes, blood vessels and other tissues affected by PXE. (medlineplus.gov)
  • Bone is composed of a calcium-based mineral complex and an organic material known as collagen. (aliveandwell.health)
  • Approximately 15% is complexed calcium bound to organic and inorganic anions, 40% is bound to albumin, and the remaining 45% circulates as free ionized calcium. (medscape.com)
  • The most organic and natural way to help your gecko is to give her a nesting site or box to help her lay her eggs. (reptilescout.com)
  • Many lines of latest proof from Rabbit Polyclonal to Keratin 20 both in vitro and in vivo research show that renal tubular cell damage can boost crystal binding on the wounded site and therefore may raise the rock risk9C13. (biopaqc.com)
  • Calcium tells our junctions to release transmitters that bind to our muscles and tell them to contract. (breetheclub.com)
  • Subunit Structures of Calcium Stations However the purified calcium mineral route protein was well behaved in lots of respects the behavior from the α subunit music group in SDS-PAGE evaluation under different experimental circumstances was unstable. (researchensemble.com)
  • Subunit framework of voltage-gated calcium mineral stations. (researchensemble.com)
  • May phosphatase activity is normally physiologically governed by calcium May B subunit (CaNB) and calmodulin (CaM) (Klee et al. (conferencedequebec.org)
  • This formula was specially created to meet the nutritional requirements of children with special needs, who may be dealing with restrictive diets, poor eating habits, or chelation therapies, all of which can result in mineral deficiencies. (autismsupplementscenter.com)
  • In patients with normal or only slightly reduced calcium levels, doses of 0.25 mcg every other day are sufficient. (medicines.org.uk)
  • If the body cannot regulate calcium levels, it can be deposited on the body's soft tissues, including the arteries. (wakeup-world.com)
  • however, it produced a greater reduction in Tc-99m PYP uptake in the infarcts, probably by complexing with Tc-99m PYP binding sites and by dilution of Tc-99m PYP in circulating diphosphonate pool. (elsevierpure.com)
  • The parent mineral rock derives inorganic materials and is found in the form of sand, silt and clay. (intechopen.com)
  • In the blood, absorbed lead is bound to erythrocytes and then is distributed initially to multiple soft tissues and eventually into bone. (cdc.gov)
  • Outcomes Enhanced crystal-cell adhesion in the Primarily restoring cell monolayers, the perfect post-scratch time-point for crystal-cell adhesion assay was described because of this present research addressing ramifications of tissues fix on crystal adhesion on the wounded site. (biopaqc.com)
  • PXE is a condition characterized by abnormal accumulation of calcium and other minerals in elastic fibers, a component of connective tissues that provide strength and flexibility to structures throughout the body. (medlineplus.gov)