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  • neurons
  • Upstream of ADF, the Gqα protein EGL-30 facilitates training-dependent induction of tph-1 by functional regulation of olfactory sensory neurons, which underscores the importance of sensory experience. (biomedsearch.com)
  • G-protein-coupled inwardly rectifying potassium (GIRK) channels contribute to the resting membrane potential of many neurons, including dopamine (DA) neurons in the ventral tegmental area (VTA). (nih.gov)
  • In conclusion, activity-dependent GIRK channel plasticity may represent a slow destabilization process favoring the switch between the two firing modes of VTA DA neurons. (nih.gov)
  • Vascular endothelial growth factor (VEGF) signaling regulates hippocampal neurons by elevation of intracellular calcium and activation of calcium/c. (nih.gov)
  • The present study was undertaken to characterize neuronal activity-dependent expression and release of vascular endothelial growth factor (VEGF) from rat hippocampal neurons and its contribution to neuronal functions. (nih.gov)
  • A brief (10 min) application of recombinant VEGF165 to neurons elicited a slow rise in cytosolic Ca2+ in a VEGFR2 dependent manner. (nih.gov)
  • mice
  • To do this a mouse model of type 2 diabetes (db/db) (n=8) alongside nDM control mice (n=11) were injected with KN93 (10 μmol/kg) every other day from the age of 14 to 18 weeks of age. (otago.ac.nz)
  • Here we have utilised an aptamer-based proteomics approach to profile 1,129 proteins in the serum of wild-type and mdx (dystrophin deficient) mice. (ox.ac.uk)
  • Measurements of nuclear and cytosolic Ca 2+ transients in IP 3 sponge mice showed no increase of Ca 2+ levels during diastole as we detected in wild-type mice. (springer.com)
  • Nuclei, isolated from ventricular myocytes of mice after chronic Ang II treatment, showed an elevation of IP 3 R2 expression which was dependent on calcineurin/NFAT signaling and persisted for 3 weeks after removal of the Ang II stimulus. (springer.com)
  • MeSH
  • In addition, the relatively rigid sarcomeric organization in striated muscle with a dense protein mesh at the Z-lines raises the possibility that local SR within a given sarcomere could serve its particular sarcomere but not necessarily communicate with neighboring sarcomeres either longitudinally or transversely. (ahajournals.org)
  • channels
  • We found that Kir6.2/SUR2A (the cardiac-type K(ATP)) channels were activated by cGMP-selective phosphodiesterase inhibitor zaprinast in a concentration-dependent manner in cell-attached patches obtained from HEK293 cells, an effect mimicked by the membrane-permeable cGMP analog 8-bromo-cGMP whereas abolished by selective PKG inhibitors. (nih.gov)
  • 1 Electrical depolarization of the cell membrane opens L-type Ca 2+ channels, causing Ca 2+ influx. (ahajournals.org)
  • Mammalian
  • Wnts are secreted signaling molecules that can transduce their signals through several different pathways.This pathway is Siah2 and APC dependent, but GSK-3 and beta-TrCP independent.Furthermore, we provide evidence that Wnt-5a also acts in vivo to promote beta-catenin degradation in regulating mammalian limb development and possibly in suppressing tumor formation. (nih.gov)
  • ubiquitin
  • Using the epithelial sodium channel (ENaC), essential for kidney control of blood pressure, we have shown that ubiquitin pathway proteins control the number of ENaCs at the cell surface resulting in changes in Na+ absorption. (otago.ac.nz)
  • antagonist
  • Therefore, the organotypic hippocampal slices were incubated with 100 µM luzindole, a MT1/2 competitive antagonist. (nih.gov)
  • Figure 6 shows images of hippocampal slices cultured with the MT1/2 receptor antagonist in presence of either the vehicle or MEL and stained with the MAP2 antibody. (nih.gov)
  • Enzymes
  • Previous work showed that MEL increased the synthesis and translocation of CaM, suggesting that MEL activates CaM-dependent enzymes by this pathway. (nih.gov)
  • These kinases belong to a diverse group of enzymes that participate in many cellular responses and are activated by increasing concentrations of intracellular Ca 2+ . (diabetesjournals.org)
  • cells
  • They observed that targeting the Ca 2+ -binding protein parvalbumin to the nucleus significantly reduced cell proliferation and altered the proportions of cells that were in the S and G 2 -M phases of the cell cycle. (biologists.org)
  • We previously reported that HDL and its major apolipoprotein, apolipoprotein AI (apoAI), activate the key metabolic regulatory enzyme AMP-activated protein kinase (AMPK) in endothelial cells and are critical for the nitric oxide-mediated vasodilatory effects of HDL. (ahajournals.org)
  • RT-PCR, Western blotting, and immunohistochemical staining analysis revealed that CaM-K kinase-α (CaM-KKα) and CaM-KIV were localized in rat pancreatic β-cells and their cell line, INS-1. (diabetesjournals.org)
  • In some cell types (nonmuscle cells), it is generally accepted that the outer nuclear membrane and ER have similar characteristics and outer nuclear membrane is continuous with ER membrane including SERCA, whereas the inner nuclear membrane is quite different from the outer nuclear membrane. (ahajournals.org)
  • Exocytosis elicited by action potentials and voltage-clamp calcium currents in individual mouse pancreatic B-cells. (ox.ac.uk)
  • D , Tonic firing of VTA DA cells depresses the amplitude of GABA B -GIRK slow IPSC ( n = 8, 2 min bin). (nih.gov)
  • exocytosis
  • Since the latter is in better agreement with the maximum rate of insulin secretion from islets (approximately 2 granules/s), we suggest that membrane retrieval may set an upper limit on the rate of exocytosis during extended periods of secretion. (ox.ac.uk)
  • membrane
  • Structural insights into the architecture and membrane interactions of the conserved COMMD proteins. (otago.ac.nz)
  • Following withdrawal of methacholine, the eGFP-PHPLCδ1 probe returned to the plasma membrane within 2 min (Fig. 6A). (nih.gov)
  • novel
  • Identification of novel, therapy-responsive protein biomarkers in a mouse model of Duchenne muscular dystrophy by aptamer-based serum proteomics. (ox.ac.uk)
  • Identification of novel protein biomarkers has been limited due to the massive complexity of the serum proteome and the presence of a small number of very highly abundant proteins. (ox.ac.uk)
  • This study has identified multiple novel, therapy-responsive protein biomarkers in the serum of the mdx mouse with potential utility in DMD patients. (ox.ac.uk)
  • transcription
  • We demonstrate that nuclear calcineurin was able to act as a nuclear Ca 2+ sensor detecting local Ca 2+ release from the nuclear envelope via IP 3 R. Nuclear calcineurin mutants defective for Ca 2+ binding failed to activate NFAT-dependent transcription. (springer.com)