Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro. (fishersci.com)
Chromatin assembly factor 1 subunit A is a protein that in humans is encoded by the CHAF1A gene. (wikipedia.org)
Here, we report on a loss-of-function mutant allele of the FASCIATA1 (FAS1) subunit of the chromatin assembly factor 1 (CAF-1) complex that suppresses the phenotype of WEE1- or ATR-deficient Arabidopsis (Arabidopsis thaliana) plants. (muni.cz)
Mammalian H3.1 and H3.2 are deposited by the heterotrimeric chromatin assembly complex 1 (CAF1), whereas H3.3 is deposited at transcriptionally active regions by the Histone Regulator A (HIRA) complex [ 15 , 16 ]. (biomedcentral.com)
CHAF1B is the p60 subunit of the chromatin assembly factor (CAF1) complex, which is responsible for assembly of histones H3.1/H4 heterodimers at the replication fork during S phase. (telethonkids.org.au)
Although initially discovered as a retinoblastoma binding protein it has an affinity for core HISTONES and is a subunit of chromatin assembly factor-1 and polycomb repressive complex 2. (lookformedical.com)
The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. (nih.gov)
Protein2
protein_coding" "Cz02g32120.t1","No alias","Chromochloris zofingiensis","B-block binding subunit of TFIIIC [Interproscan]. (ntu.edu.sg)
It is found as a subunit of protein complexes that are in involved in the enzymatic modification of histones including the Mi2 and Sin3 histone deacetylase complexes and the polycomb repressive complex 2. (lookformedical.com)
P1501
MIM 601245), and p150 (CHAF1A) subunits that assembles histone tetramers onto replicating DNA in vitro (Kaufman et al. (wikipedia.org)
CHAF1B1
Reducing Chaf1b activity by either heterozygous deletion or overexpression of a CAF1 dominant negative allele is sufficient to suppress leukemogenesis in vivo without impairing normal hematopoiesis. (telethonkids.org.au)
CCR42
This is consistent with the localization of other members of the CAF1/CCR4/NOT complex in Plasmodium, model eukaryotes, and human cells. (pberghei.eu)
Temme C, Zaessinger S, Meyer S, Simonelig M, Wahle E. A complex containing the CCR4 and CAF1 proteins is involved in the posterior cortex by the same direction and stuck to a coverslip using heptane glue and embryos prandin 2 mg price were fixed in a common cytoplasm until the pole cells. (squabash.com)
Transcription1
In the alternative IκB-independent pathway, selleck inhibitor direct phosphorylation of NF-κB subunit p65 by IKK also modulates NF-κB transcription activity [31]. (plasignaling.com)
Putative1
Cac2, a putative subunit of H3.1 deposition complex CAF1, is not required for growth, whereas the expression of the putative ortholog of the H3.3-specific chaperone Hir1 is essential in Tetrahymena . (biomedcentral.com)
HIRA1
We report the crystal structure of an ASF1a-HIRA heterodimer and a biochemical dissection of ASF1a's mutually exclusive interactions with HIRA and the p60 subunit of CAF-1. (omicsdi.org)
Molecular1
It is comprised of three different subunits of 48, 60, and 150 kDa molecular size. (lookformedical.com)
Chromatin2
Chromatin assembly factor 1 subunit A is a protein that in humans is encoded by the CHAF1A gene. (wikipedia.org)
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. (nih.gov)
Ribonuclease activity2
The complex is composed by two subunits with ribonuclease activity (Caf1 and Ccr4) and at least six non-catalytic subunits. (nottingham.ac.uk)
In addition to six non-catalytic components, the complex contains two enzymatic subunits with ribonuclease activity: Ccr4 and Caf1 (Pop2). (bvsalud.org)
CNOT7 and CNOT82
In vertebrate cells, the duplication of the catalytic subunits Caf1 (encoded by CNOT7 and CNOT8) and Ccr4 (encoded by CNOT6 and CNOT6L) lead to the heterogeneity of the complex. (nottingham.ac.uk)
In vertebrates, each deadenylase subunit is encoded by two paralogues: Caf1, which can interact with the anti-proliferative protein BTG2, is encoded by CNOT7 and CNOT8, whereas Ccr4 is encoded by the highly similar genes CNOT6 and CNOT6L. (bvsalud.org)
P1504
MIM 601245), and p150 (CHAF1A) subunits that assembles histone tetramers onto replicating DNA in vitro (Kaufman et al. (wikipedia.org)
The non-catalytic subunits are organised in modules, each with a specific function, allowing the recruitment of the complex on specific mRNAs. (nottingham.ac.uk)
This study discovered and explored the interactome of the catalytic subunits Caf1 and Ccr4 and detected new interacting partners that may recruit the deadenylase complex on specific mRNAs. (nottingham.ac.uk)
Well-characterized enzymes involved in the shortening of the poly(A) tail include the multi-subunit Ccr4-Not deadenylase, which contains the Caf1 (Pop2) and Ccr4 catalytic components, and poly(A)-specific ribonuclease (PARN). (bvsalud.org)
Currently, it is unclear whether the catalytic subunits work co-operatively or whether the nuclease components have unique roles in deadenylation. (bvsalud.org)
We demonstrate the utility of the assay by screening a chemical compound library, resulting in the identification of non-nucleoside inhibitors of the Caf1/CNOT7 enzyme, a catalytic subunit of the Ccr4-Not deadenylase complex. (bvsalud.org)
Deadenylase2
Finally, phylogenetic analysis of 15 mammalian species identified different evolutionary rates acting on the paralogous deadenylase subunits. (nottingham.ac.uk)
These compounds may be useful tools for the biochemical analysis of the Caf1/CNOT7 deadenylase subunit of the Ccr4-Not complex and indicate the feasibility of developing selective inhibitors of deadenylase enzymes using the fluorescence-based assay. (bvsalud.org)
Ccr44
We therefore developed a method to express and purify a minimal human BTG2-Caf1-Ccr4 nuclease sub-complex from bacterial cells. (bvsalud.org)
By using chemical inhibition and well-characterized inactivating amino acid substitutions, we demonstrate that the enzyme activities of Caf1 and Ccr4 are both required for deadenylation in vitro. (bvsalud.org)
These results indicate that Caf1 and Ccr4 cooperate in mRNA deadenylation and suggest that the enzyme activities of Caf1 and Ccr4 are regulated via allosteric interactions within the nuclease module. (bvsalud.org)
This is consistent with the localization of other members of the CAF1/CCR4/NOT complex in Plasmodium, model eukaryotes, and human cells. (pberghei.eu)
Yeast Scd6 interacts with Dcp2 and Dhh1, respectively a subunit and a regulator of the mRNA decapping enzyme, and also associates with translation initiation factor eIF4G to inhibit translation in cell extracts. (nih.gov)
Interactions1
We report the crystal structure of an ASF1a-HIRA heterodimer and a biochemical dissection of ASF1a's mutually exclusive interactions with HIRA and the p60 subunit of CAF-1. (omicsdi.org)
Domain1
Caf1A usher possesses a Caf1 subunit-like domain that is crucial for Caf1 fibre secretion. (expasy.org)