Studies of viral attachment to cells on porous collagen-I coated microcarrier beads under cultured gastrointestinal epithelial cells (Caco-2) using conditions of physiological fluid shear in rotating wall ves- recombinant virus-like particles or infectious noroviruses sel bioreactors. (cdc.gov)
Inhibitory effects of Galega officinalis on glucose transport across monolayers of human intestinal epithelial cells (Caco-2). (naturalnews.com)
Here we investigated whether polarized monolayers of intestinal epithelial cells might regulate inflammatory responses by secreting IL-8 in a vectorial fashion (i.e. apical versus basolateral) depending on the location of the TLR stimulus. (biomedcentral.com)
The CXCR1 receptor for IL-8 was expressed only on the apical membrane of Caco-2 BBE cells and differentiated epithelial cells in the human small intestine and colon. (biomedcentral.com)
These results show that IL-8 induces autocrine signalling via an apical CXCR1 in Caco-2 BBE intestinal epithelial cells and that this receptor is also expressed on the apical surface of differentiated human intestinal epithelial cells in vivo , suggesting an autocrine function for IL-8 secreted in the lumen. (biomedcentral.com)
Cells20
Caco-2 cells and Biopharmaceutics Classification System (BCS) for prediction of transepithelial transport of xenobiotics (model drug: caffeine). (nel.edu)
Smetanova L, Stetinova V, Kholova D, Kvetina J, Smetana J, Svoboda Z. Caco-2 cells and Biopharmaceutics Classification System (BCS) for prediction of transepithelial transport of xenobiotics (model drug: caffeine). (nel.edu)
Both TC7 and Caco-2p cells expressed mRNA for Pgp and several important CYPs. (nih.gov)
Ablation of DNMT3A in Caco-2 cells results in global DNA hypomethylation, which is linked to impaired regenerative capacity, transepithelial resistance and intercellular junction formation. (nature.com)
We have begun to establish 3D in vitro human infection models based on Caco-2 and primary intestinal cells mimicking the gastrointestinal barrier. (uni-wuerzburg.de)
None of the tested forms induced a pro-inflammatory response in Caco-2 cells or in macrophages either. (springer.com)
Transcriptome analyses revealed that Caco-2 BBE cells respond to stimulation with IL-8 supporting the hypothesis that IL-8 induces G protein-coupled receptor signalling. (biomedcentral.com)
However, it is not known if CXCR1 is expressed on the apical or basolateral poles in polarized Caco-2 BBE cells or human intestinal tissue or whether the direction of TLR signalling influences the direction of IL-8 secretion. (biomedcentral.com)
Here we investigated IL-8 secretion in response to TLR signalling using intestinal Caco-2 BBE cells grown in the Transwell system. (biomedcentral.com)
Additionally, we determined the localization of the CXCR1 receptor in Caco-2 BBE cells and human intestinal tissue samples and investigated the role of IL-8 autocrine signalling using transcriptomics. (biomedcentral.com)
Stimulation of polarized Caco-2 BBE cells with Pam2CSK4 and Pam3CSK4, agonists for TLR2/6 and TLR2/1 from the apical or basolateral sides induced secretion of IL-8 predominantly into the apical compartment regardless of the location of the stimulus, although basolateral stimulation induced smaller amounts of IL-8 compared to apical stimulation. (biomedcentral.com)
Evaluation of the interactions between multiwalled carbon nanotube s and Caco-2 cells. (cdc.gov)
Caco-2 cells were exposed to 50 µg/ml MWCNT (oxidized or pristine) for 24 h, and experiments were repeated in the presence of 2.5 mg/L natural organic matter. (cdc.gov)
1 Monolayers of Caco‐2 cells, a transformed cell line derived from human colorectal carcinoma, have been widely used by pharmaceutical companies as a standard intestinal barrier model for the prediction of intestinal permeability for drug candidates. (sigmaaldrich.com)
2 Despite their convenience and accessibility, Caco-2 cells have a single homogeneous phenotype, and therefore fail to recapitulate lineage development into the diverse cell phenotypes that constitute the physiology of the native intestinal epithelium, leading to inconclusive experimental results. (sigmaaldrich.com)
Furthermore, we have demonstrated that our scaffolds support the growth and differentiation of intestinal epithelial Caco-2 cells up to 21 days, obtaining a matured epithelial monolayer with effective tissue barrier properties. (ibecbarcelona.eu)
Then, we cultured Caco-2 cells on top of the 3T3-NIH fibroblast-laden hydrogels up to 21 days. (ibecbarcelona.eu)
Our preliminary results showed that the co-culture of Caco-2 cells with 3T3-NIH fibroblasts favours the epithelial cell growth and improves their barrier function. (ibecbarcelona.eu)
This activity varied according to concentration (100/500 μg/ml), multiplicity of infection (0.1/0.01), and cell type (Vero E6/Caco-2 cells). (frontiersin.org)
Apical5
However, irrespective of the loaded concentration and exposure time, the permeabilities were very low from apical to basolateral compartments (from 4 to 15 × 10−8 cm·s−1) and from basolateral to apical compartments (from 2 to 37 × 10−8 cm·s−1). (hal.science)
The rate of transport of terfenadine and its metabolites hydroxy-terfenadine (HO-T) and azacyclonol across TC7 monolayers was 7.1-, 3.5- and 2.1-fold higher, respectively, in the basolateral to apical direction than it was in the apical to basolateral (AP-BL) direction. (nih.gov)
Transport of ambroxol (100, 300 and 1000 µmol/l) was studied in a human colon carcinoma cell line Caco-2 in apical to basolateral and basolateral to apical direction, under iso-pH 7.4 and pH-gradient (6 vs. 7.4) conditions. (elis.sk)
In the Caco-2 BBE model of polarized villus-like epithelium, apical stimulation with TLR2 and TLR5 ligands resulted in the apical secretion of IL-8. (biomedcentral.com)
Dissolution is measured in both solutions, and permeation is measured using chambers containing Caco-2 cell monolayers with their apical surface facing the dissolution chamber. (absorption.com)
Tight Junctions5
The aim of this study was thus to determine the effect of nicotine, the major constituent in cigarettes and its metabolites on the integrity of tight junctions in Caco-2 cell monolayers. (ulster.ac.uk)
The integrity of Caco-2 tight junctions was analysed by measuring the transepithelial electrical resistance (TER) and by tracing the flux of the fluorescent marker fluorescein, after treatment with various concentrations of nicotine or nicotine metabolites over 48 h. (ulster.ac.uk)
In this study, our outcomes also identified that escalating temperature (373uC) reduces TEER and increases functional permeability for the substantial molecules of HRP in Caco-2 monolayers, indicating the physical impairment of tight junctions. (trpv1inhibitor.com)
ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. (thermofisher.com)
Barrier3
Several functional components of the epithelium contribute to this unique role, among them the secretion of a mucus layer and life-long regeneration of the different epithelial cell types from a stem cell compartment, allowing for quick repair of barrier defects 2 . (nature.com)
The µD/P model and the µFLUX model, using a Caco‐2 cell monolayer/PAMPA membrane as the permeation barrier, respectively, were compared in terms of robustness and ease of handling, while studying the drug supersaturation-precipitation-permeation interplay. (pion-inc.com)
Our in vitro tool IDAS is a dissolution vessel, which uses the Caco-2 colon carcinoma cell line as the biorelevant barrier to evaluate the dissolution and permeation of new drug compounds simultaneously. (absorption.com)
Permeation1
In vitro permeation assay techniques such as Caco-2, MDCK cell lines, PAMPA, tissue based assays like the intestinal everted sac, intestinal single pass perfusion, Thiry-Vella loops are being used [ 6 - 17 ]. (omicsonline.org)
Enterocytes1
The aim of this study was to determine whether multiwalled carbon nanotube s (MWNCT) are taken up by and are toxic to human intestinal enterocytes using the Caco-2 cell model. (cdc.gov)
Intestine2
The Caco-2 cell line was used as a model to determine if the type of fatty acid, unsaturated versus saturated, either suppresses or enhances the cellular acquisition (uptake) and transport of 59**Fe in the human intestine. (usda.gov)
The major challenge in the oral drug delivery of poorly permeable drug is their transport across intestine [ 1 , 2 ]. (omicsonline.org)
Membrane1
Membrane enriched extracts (40 µg lysate) of Caco-2 (Lane 1), Caco-2 treated with IFN Gamma (50 ng/mL for 24hrs) (Lane 2), A-431 (Lane 3), HEL 92.1.7 (Lane 4) and Jurkat (Lane 5). (thermofisher.com)
Permeability2
As intestinal permeability is a key parameter of oral toxicokinetics, the apparent permeability of MC-RR across a differentiated intestinal Caco-2 cell monolayer was investigated. (hal.science)
Thus, BCS class 4 Cefixime can be treated as bcs class 2 by increasing its solubility instead of permeability as evident by ex-vivo and in vivo study. (omicsonline.org)
Integrity3
Caco-2 cell monolayer integrity and effect of probiotic Escherichia coli Nissle 1917 components. (nel.edu)
Stetinova V, Smetanova L, Kvetina J, Svoboda Z, Zidek Z, Tlaskalova-Hogenova H. Caco-2 cell monolayer integrity and effect of probiotic Escherichia coli Nissle 1917 components. (nel.edu)
The iron containing medium and/or fatty acids had varied effects on monolayer integrity and paracellular transport. (usda.gov)
MRNA2
mRNA expression of eight CYPs and Pgp was investigated in TC7 and parental Caco-2 (Caco-2p) cell monolayers using RT-PCR. (nih.gov)
CXCR1 is expressed in the human IEC line Caco-2 BBE [ 3 ], which is in agreement with our own microarray data using Caco-2 BBE cell mRNA (see below). (biomedcentral.com)
Assays2
Here we demonstrate the use of highly-characterized human iPS cell-derived colon organoids as an alternative cell model for traditional Caco-2 cell drug toxicity assays for drug screening applications. (sigmaaldrich.com)
For this purpose, we performed in vitro assays, proving that Lf exerts an antiviral activity against SARS-COV-2 through direct attachment to both SARS-CoV-2 and cell surface components. (frontiersin.org)
Model3
The Caco-2 cell monolayer model is widely used as a standard screening tool for studying the mechanisms of cellular drug trans. (nel.edu)
In vitro differentiation of small intestinal epithelium that nausea, vomiting, and watery diarrhea within 12-24 hours approaches physiologic functionality of the in vivo host of exposure and typically remain symptomatic for 1-2 may allow for the development of a pathogenesis model days ( 5 ). (cdc.gov)
To further characterize cytochrome P450 (CYP) and P-glycoprotein (Pgp) expression in monolayers of the Caco-2 cell clone TC7, a cell culture model of the human intestinal epithelium. (nih.gov)
Incubate organoids for 1 day at 37 °C, 5% CO 2 . (sigmaaldrich.com)
Experiments3
TJ proteins have been shown (A, D): occludin (B), ZO-1 (C) and claudin-2 (E). Outcomes had been cIAP-2 custom synthesis reported as signifies six SD from 3 independent experiments. (trpv1inhibitor.com)
In this work, we analyze expression patterns of DNMT3A in patients with IBD, perform loss of function experiments using Caco-2 cell line and generate a conditional Dnmt3a ΔIEC mouse line. (nature.com)
Wild-type and St2 −/− mice were used in wound healing experiments and in two experimental models of IBD triggered by 2,4,6-trinitrobenzene sulphonic acid or dextran sodium sulphate (DSS). (bmj.com)
Protein4
IL-8 can signal through two receptors, CXCR1 and CXCR2 both of which are members of the G-protein-coupled receptor (GPCR) family [ 2 ]. (biomedcentral.com)
In neutrophils, IL-8 binding to CXCR1 triggers G protein-coupled signalling and formation of second messengers that mediate cellular migration, exocytosis of effectors and a respiratory burst to facilitate oxygen-dependent killing of phagocytosed microorganisms [ 2 ]. (biomedcentral.com)
The TJ protein occludin and ZO-1 were disrupted in anoxia-injured monolayer. (ecco-ibd.eu)
Synthetic peptide derived from the central portion of the ZO-2 protein. (thermofisher.com)
Transport3
The transport, metabolism and efflux of terfenadine and its metabolites were investigated in TC7 monolayers. (nih.gov)
The monomers and dimers were able to transport through the 528-48-3 Caco-2 monolayer at a rate of 10.45% and 6.4%, respectively. (columbiagypsy.net)
transepithelial transport in Caco-2 cell monolayers showed intermediate in vivo transport and absorption for three (β-LG f 87-91 , β-LG f 91-99 , and β-LG f 158-164 ). (ku.dk)
Infection2
Moreover, small animal infection models of S . Typhimurium infection do not accurately reflect the innate immune response of the human host due to differences in disease progression and pattern recognition [2, 3]. (uni-wuerzburg.de)
The well-known antiviral activity of Lf has been demonstrated against DNA, RNA, and enveloped and naked viruses and, therefore, Lf could be efficient in counteracting also SARS-CoV-2 infection. (frontiersin.org)
Cell lines1
Caco-2 cell lines in drug discovery- an updated perspective. (sigmaaldrich.com)
Effects1
difficulties during formulation and adverse effects and toxicity [ 2 - 4 ]. (ijpsonline.com)
Drug1
Among the pharmacokinetic parameters, the volume of distribution is a key determinant of the effective half-life ( t 1/2 ) and dosing interval of a prospective drug. (aspetjournals.org)
Exposure1
Caco-2 monolayers had been cultured for 24 h following 1 h of heat exposure without having (37uC group and 43uC group) or with PUFAs pre-incubation for 96 h. (trpv1inhibitor.com)
Forms1
ZO-1 forms complexes with either ZO-2 or ZO-3. (thermofisher.com)